RchiOBHm_Chr6g0310871

Protein of unknown function (DUF1230)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0008080
Physical Location & Seq
Reverse (-)
68067582 .. 68069151
1570 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27954

Sequence Viewer

Length: 765 bp
ATGGCCACCGCACTTGTGCTACACCCAACCCCAAATTTCCTCTCACCAATCCCTCCAACCCCAAATCCCAGACTTCACCGCCACCGCTACCGCACCGAAATACCCAAAATACCCTTCTCCTCGTCGTCGTTCCGAAACGGCGGCCCAGCAGAAACAGAGTGCCCTGTTCCGCTGGAGCAGCAGCCCATAAACGAGTACCAGAACCTCTCCACCTCCTTCCCCTTCTCGTGGGCCTCCGGCGACCTCGTCGAGTACTGCTCTCGCCTGATCGCCACCGGCGCCTCCTTCGCGCTCTTCGTGGGCCTCCCCGTCGCCTCCTTCGGCGTCTTCGGGGCCCAATCGGAGCCGCTCAAGCAGTCCCTCTACGCAGTTTCCGGTGGGTTTTTGGTGGTCACTCTCGCCGTGGTGAGGATGTACCTCGGTTGGGCTTACGTCGGCAACCGCTTGCTTAGTGCCACTGTTGAATACGAAGAGACTGGGTGGTATGATGGTCAGATATGGGTTAAGACTGCTGAAGTTTTGGCGCGTGATCGGCTTCTTGGTTCATTTTCTGTGAAGCCTGTGCTGAGCAGATTGAAGTTAACGCTGGTGACTCTTGCAGCATCAATACTTGCATGTGTTCTCGTCCTCCTTTACATTGATGGAGGCCAAATCCAAGAAGCCGAGAATCGCGTTATACGTGGAGTTTACAACGATGATTCTGCAAGATCGTTTGAGCCCGATGCGTTTTGTGGTGGTGATCCTGGTCCTGGTCCTCCCCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1230

Protein Analysis

254

Amino Acids

27.67

Weight (kDa)

5.66

Isoelectric Point (pI)

41.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CGLD27-like PF06799 54 - 197 3.1e-54 Conserved in the green lineage and diatoms 27
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 278
AccBSI CCGCTC 1 cut(s) 349
AccII CGCG 3 cut(s) 290, 526, 672
AciI CCGC 8 cut(s) 9, 79, 85, 91, 141, 170, 347, 442
AclWI GGATC 1 cut(s) 734
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 34
AcuI CTGAAG 1 cut(s) 534
AcyI GRCGYC 2 cut(s) 279, 324
AfaI GTAC 3 cut(s) 197, 254, 416
AfiI CCNNNNNNNGG 4 cut(s) 228, 408, 424, 749
AgsI TTSAA 2 cut(s) 464, 577
AjnI CCWGG 2 cut(s) 742, 748
AjuI GAANNNNNNNTTGG 2 cut(s) 98, 130
Alw26I GTCTC 1 cut(s) 467
AlwI GGATC 1 cut(s) 734
AoxI GGCC 6 cut(s) 3, 142, 231, 301, 333, 646
ApaI GGGCCC 1 cut(s) 337
ApeKI GCWGC 3 cut(s) 178, 181, 599
ApoI RAATTY 1 cut(s) 34
AspLEI GCGC 3 cut(s) 281, 292, 526
AspS9I GGNCC 7 cut(s) 143, 231, 301, 333, 334, 746, 752
AsuHPI GGTGA 5 cut(s) 36, 68, 418, 601, 749
AvaII GGWCC 2 cut(s) 746, 752
BaeGI GKGCMC 2 cut(s) 164, 337
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 278
BanII GRGCYC 2 cut(s) 337, 720
BauI CACGAG 1 cut(s) 226
BbsI GAAGAC 1 cut(s) 319
BbvI GCAGC 3 cut(s) 190, 193, 611
BccI CCATC 2 cut(s) 482, 635
BceAI ACGGC 2 cut(s) 154, 386
BcgI CGANNNNNNTGC 2 cut(s) 683, 717
BciT130I CCWGG 2 cut(s) 744, 750
BcoDI GTCTC 1 cut(s) 467
BfoI RGCGCY 1 cut(s) 282
BisI GCNGC 5 cut(s) 142, 179, 182, 347, 600
BlpI GCTNAGC 1 cut(s) 566
BlsI GCNGC 5 cut(s) 143, 180, 183, 348, 601
BmcAI AGTACT 1 cut(s) 254
Bme1390I CCNGG 2 cut(s) 744, 750
Bme18I GGWCC 2 cut(s) 746, 752
BmgT120I GGNCC 7 cut(s) 143, 231, 301, 333, 334, 746, 752
BmiI GGNNCC 4 cut(s) 280, 334, 335, 345
BmrFI CCNGG 2 cut(s) 744, 750
BmrI ACTGGG 1 cut(s) 486
BmsI GCATC 2 cut(s) 611, 712
BmuI ACTGGG 1 cut(s) 486
BpiI GAAGAC 1 cut(s) 319
BplI GAGNNNNNCTC 2 cut(s) 242, 274
BpmI CTGGAG 1 cut(s) 194
Bpu1102I GCTNAGC 1 cut(s) 566
BpuEI CTTGAG 1 cut(s) 335
BsaAI YACGTR 1 cut(s) 680
BsaHI GRCGYC 2 cut(s) 279, 324
BsaJI CCNNGG 2 cut(s) 402, 418
BsaWI WCCGGW 1 cut(s) 374
Bsc4I CCNNNNNNNGG 4 cut(s) 228, 408, 424, 749
Bse118I RCCGGY 1 cut(s) 275
Bse1I ACTGG 1 cut(s) 481
BseBI CCWGG 2 cut(s) 744, 750
BseDI CCNNGG 2 cut(s) 402, 418
BseGI GGATG 1 cut(s) 417
BseLI CCNNNNNNNGG 4 cut(s) 228, 408, 424, 749
BseMII CTCAG 1 cut(s) 557
BseNI ACTGG 1 cut(s) 481
BseRI GAGGAG 1 cut(s) 109
BseSI GKGCMC 2 cut(s) 164, 337
BseXI GCAGC 3 cut(s) 190, 193, 611
BseYI CCCAGC 1 cut(s) 145
Bsh1236I CGCG 3 cut(s) 290, 526, 672
BshFI GGCC 6 cut(s) 5, 144, 233, 303, 335, 648
BshNI GGYRCC 1 cut(s) 278
BsiSI CCGG 3 cut(s) 237, 276, 375
BslFI GGGAC 1 cut(s) 343
BslI CCNNNNNNNGG 4 cut(s) 228, 408, 424, 749
BsmAI GTCTC 1 cut(s) 467
BsmFI GGGAC 1 cut(s) 343
BsnI GGCC 6 cut(s) 5, 144, 233, 303, 335, 648
Bsp120I GGGCCC 1 cut(s) 333
Bsp1286I GDGCHC 3 cut(s) 164, 337, 720
Bsp143I GATC 4 cut(s) 267, 529, 707, 739
Bsp1720I GCTNAGC 1 cut(s) 566
BspACI CCGC 8 cut(s) 9, 79, 85, 91, 141, 170, 347, 442
BspANI GGCC 6 cut(s) 5, 144, 233, 303, 335, 648
BspCNI CTCAG 1 cut(s) 558
BspFNI CGCG 3 cut(s) 290, 526, 672
BspLI GGNNCC 4 cut(s) 280, 334, 335, 345
BspPI GGATC 1 cut(s) 734
BspQI GCTCTTC 1 cut(s) 299
BspT107I GGYRCC 1 cut(s) 278
BsrBI CCGCTC 1 cut(s) 349
BsrFI RCCGGY 1 cut(s) 275
BsrI ACTGG 1 cut(s) 481
BssAI RCCGGY 1 cut(s) 275
BssECI CCNNGG 2 cut(s) 402, 418
BssMI GATC 4 cut(s) 267, 529, 707, 739
BssNI GRCGYC 2 cut(s) 279, 324
BssSI CACGAG 1 cut(s) 226
Bst2BI CACGAG 1 cut(s) 226
Bst2UI CCWGG 2 cut(s) 744, 750
Bst4CI ACNGT 1 cut(s) 460
Bst6I CTCTTC 2 cut(s) 299, 465
BstACI GRCGYC 2 cut(s) 279, 324
BstBAI YACGTR 1 cut(s) 680
BstC8I GCNNGC 1 cut(s) 446
BstDEI CTNAG 2 cut(s) 449, 566
BstDSI CCRYGG 1 cut(s) 402
BstF5I GGATG 1 cut(s) 417
BstFNI CGCG 3 cut(s) 290, 526, 672
BstH2I RGCGCY 1 cut(s) 282
BstHHI GCGC 3 cut(s) 281, 292, 526
BstKTI GATC 4 cut(s) 270, 532, 710, 742
BstMAI GTCTC 1 cut(s) 467
BstMBI GATC 4 cut(s) 267, 529, 707, 739
BstMWI GCNNNNNNNGC 5 cut(s) 178, 278, 287, 352, 532
BstNI CCWGG 2 cut(s) 744, 750
BstNSI RCATGY 1 cut(s) 618
BstSCI CCNGG 2 cut(s) 742, 748
BstSLI GKGCMC 2 cut(s) 164, 337
BstUI CGCG 3 cut(s) 290, 526, 672
BstV1I GCAGC 3 cut(s) 190, 193, 611
BstV2I GAAGAC 1 cut(s) 319
BsuRI GGCC 6 cut(s) 5, 144, 233, 303, 335, 648
BtgI CCRYGG 1 cut(s) 402
BtsCI GGATG 1 cut(s) 417
BtsIMutI CAGTG 1 cut(s) 456
Cac8I GCNNGC 1 cut(s) 446
CfoI GCGC 3 cut(s) 281, 292, 526
Cfr10I RCCGGY 1 cut(s) 275
Cfr13I GGNCC 7 cut(s) 143, 231, 301, 333, 334, 746, 752
CseI GACGC 1 cut(s) 313
Csp6I GTAC 3 cut(s) 196, 253, 415
CspCI CAANNNNNGTGG 1 cut(s) 30
CviAII CATG 1 cut(s) 615
CviQI GTAC 3 cut(s) 196, 253, 415
DdeI CTNAG 2 cut(s) 449, 566
DinI GGCGCC 1 cut(s) 280
DpnI GATC 4 cut(s) 269, 531, 709, 741
DpnII GATC 4 cut(s) 267, 529, 707, 739
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 299, 465
EarI CTCTTC 2 cut(s) 299, 465
Eco24I GRGCYC 2 cut(s) 337, 720
Eco47I GGWCC 2 cut(s) 746, 752
Eco57I CTGAAG 1 cut(s) 534
EcoO109I RGGNCCY 1 cut(s) 333
EcoRII CCWGG 2 cut(s) 742, 748
EcoT38I GRGCYC 2 cut(s) 337, 720
EgeI GGCGCC 1 cut(s) 280
EheI GGCGCC 1 cut(s) 280
FaeI CATG 1 cut(s) 618
FaiI YATR 5 cut(s) 188, 486, 499, 616, 677
FaqI GGGAC 1 cut(s) 343
FatI CATG 1 cut(s) 614
Fnu4HI GCNGC 5 cut(s) 142, 179, 182, 347, 600
FokI GGATG 1 cut(s) 424
FriOI GRGCYC 2 cut(s) 337, 720
Fsp4HI GCNGC 5 cut(s) 142, 179, 182, 347, 600
GlaI GCGC 3 cut(s) 280, 291, 525
GluI GCNGC 5 cut(s) 142, 179, 182, 347, 600
GsaI CCCAGC 1 cut(s) 149
GsuI CTGGAG 1 cut(s) 194
HaeII RGCGCY 1 cut(s) 282
HaeIII GGCC 6 cut(s) 5, 144, 233, 303, 335, 648
HapII CCGG 3 cut(s) 237, 276, 375
HgaI GACGC 1 cut(s) 313
HhaI GCGC 3 cut(s) 281, 292, 526
Hin1I GRCGYC 2 cut(s) 279, 324
Hin1II CATG 1 cut(s) 618
Hin6I GCGC 3 cut(s) 279, 290, 524
HinP1I GCGC 3 cut(s) 279, 290, 524
HincII GTYRAC 1 cut(s) 582
HindII GTYRAC 1 cut(s) 582
HinfI GANTC 3 cut(s) 592, 667, 698
HpaI GTTAAC 1 cut(s) 582
HpaII CCGG 3 cut(s) 237, 276, 375
HphI GGTGA 5 cut(s) 36, 68, 418, 601, 749
Hpy166II GTNNAC 2 cut(s) 582, 688
Hpy188I TCNGA 3 cut(s) 134, 343, 495
Hpy8I GTNNAC 2 cut(s) 582, 688
Hpy99I CGWCG 5 cut(s) 127, 130, 251, 314, 437
HpyAV CCTTC 5 cut(s) 124, 226, 232, 295, 328
HpyCH4III ACNGT 1 cut(s) 460
HpyCH4IV ACGT 2 cut(s) 432, 679
HpyCH4V TGCA 3 cut(s) 599, 614, 704
HpyF10VI GCNNNNNNNGC 5 cut(s) 178, 278, 287, 352, 532
HpyF3I CTNAG 2 cut(s) 449, 566
HpySE526I ACGT 2 cut(s) 432, 679
Hsp92I GRCGYC 2 cut(s) 279, 324
Hsp92II CATG 1 cut(s) 618
HspAI GCGC 3 cut(s) 279, 290, 524
KasI GGCGCC 1 cut(s) 278
KspAI GTTAAC 1 cut(s) 582
Kzo9I GATC 4 cut(s) 267, 529, 707, 739
LguI GCTCTTC 1 cut(s) 299
LmnI GCTCC 2 cut(s) 175, 343
Lsp1109I GCAGC 3 cut(s) 190, 193, 611
LweI GCATC 2 cut(s) 611, 712
MaeII ACGT 2 cut(s) 432, 679
MaeIII GTNAC 2 cut(s) 391, 589
MalI GATC 4 cut(s) 269, 531, 709, 741
MbiI CCGCTC 1 cut(s) 349
MboI GATC 4 cut(s) 267, 529, 707, 739
MboII GAAGA 3 cut(s) 286, 319, 482
MhlI GDGCHC 3 cut(s) 164, 337, 720
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 34
MluNI TGGCCA 1 cut(s) 5
Mly113I GGCGCC 1 cut(s) 279
MlyI GAGTC 1 cut(s) 586
MmeI TCCRAC 1 cut(s) 80
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 504, 581
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 172
MspI CCGG 3 cut(s) 237, 276, 375
MspR9I CCNGG 2 cut(s) 744, 750
MvaI CCWGG 2 cut(s) 744, 750
MvnI CGCG 3 cut(s) 290, 526, 672
MwoI GCNNNNNNNGC 5 cut(s) 178, 278, 287, 352, 532
NarI GGCGCC 1 cut(s) 279
NdeII GATC 4 cut(s) 267, 529, 707, 739
NlaIII CATG 1 cut(s) 618
NlaIV GGNNCC 4 cut(s) 280, 334, 335, 345
NmeAIII GCCGAG 1 cut(s) 688
NmuCI GTSAC 2 cut(s) 391, 589
NspI RCATGY 1 cut(s) 618
PciSI GCTCTTC 1 cut(s) 299
PcsI WCGNNNNNNNCGW 2 cut(s) 294, 676
PfeI GAWTC 2 cut(s) 667, 698
PflFI GACNNNGTC 1 cut(s) 245
PkrI GCNGC 5 cut(s) 143, 180, 183, 348, 601
PleI GAGTC 1 cut(s) 586
PluTI GGCGCC 1 cut(s) 282
PpsI GAGTC 1 cut(s) 586
Ppu21I YACGTR 1 cut(s) 680
Psp6I CCWGG 2 cut(s) 742, 748
PspFI CCCAGC 1 cut(s) 145
PspGI CCWGG 2 cut(s) 742, 748
PspN4I GGNNCC 4 cut(s) 280, 334, 335, 345
PspOMI GGGCCC 1 cut(s) 333
PspPI GGNCC 7 cut(s) 143, 231, 301, 333, 334, 746, 752
PsyI GACNNNGTC 1 cut(s) 245
RsaI GTAC 3 cut(s) 197, 254, 416
RsaNI GTAC 3 cut(s) 196, 253, 415
SapI GCTCTTC 1 cut(s) 299
SaqAI TTAA 2 cut(s) 504, 581
SatI GCNGC 5 cut(s) 142, 179, 182, 347, 600
Sau3AI GATC 4 cut(s) 267, 529, 707, 739
Sau96I GGNCC 7 cut(s) 143, 231, 301, 333, 334, 746, 752
ScaI AGTACT 1 cut(s) 254
SchI GAGTC 1 cut(s) 586
ScrFI CCNGG 2 cut(s) 744, 750
SduI GDGCHC 3 cut(s) 164, 337, 720
SetI ASST 6 cut(s) 207, 215, 246, 420, 435, 682
SfaNI GCATC 2 cut(s) 611, 712
SfoI GGCGCC 1 cut(s) 280
SgrAI CRCCGGYG 1 cut(s) 275
SinI GGWCC 2 cut(s) 746, 752
SmlI CTYRAG 1 cut(s) 350
SmoI CTYRAG 1 cut(s) 350
Sse9I AATT 1 cut(s) 34
SsiI CCGC 8 cut(s) 9, 79, 85, 91, 141, 170, 347, 442
SspDI GGCGCC 1 cut(s) 278
StyD4I CCNGG 2 cut(s) 742, 748
TaaI ACNGT 1 cut(s) 460
TaiI ACGT 2 cut(s) 435, 682
TaqI TCGA 1 cut(s) 249
TasI AATT 1 cut(s) 34
TatI WGTACW 1 cut(s) 252
TauI GCSGC 2 cut(s) 144, 349
TfiI GAWTC 2 cut(s) 667, 698
Tru1I TTAA 2 cut(s) 504, 581
Tru9I TTAA 2 cut(s) 504, 581
TscAI CASTG 1 cut(s) 463
TseFI GTSAC 2 cut(s) 391, 589
TseI GCWGC 3 cut(s) 178, 181, 599
Tsp45I GTSAC 2 cut(s) 391, 589
TspDTI ATGAA 1 cut(s) 534
TspRI CASTG 1 cut(s) 463
Tth111I GACNNNGTC 1 cut(s) 245
VpaK11BI GGWCC 2 cut(s) 746, 752
XapI RAATTY 1 cut(s) 34
XceI RCATGY 1 cut(s) 618
ZrmI AGTACT 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.