Rh6DG500100

Protein of unknown function (DUF1230)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0008080
Physical Location & Seq
Reverse (-)
65647023 .. 65648733
1711 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG500100.1

Sequence Viewer

Length: 552 bp
ATGGCCACCGCACTTCTTCTACACCCAACCCCAAATTTCCTCTCACCAATCCCTCCAACCCCAAATCCCAGACTTCACCACTACCGCACCAAAATACCCTTCTCGTCGTCGTCGTCGTTCCGAAACGGCGGCCCAGCAGAAACAGAGTGCCCTGTTCCGCTGGAGCAGCAGCCCATAAACGAGTACCAGAACCTCTCCACCTCCTTCCCCTTCTCGTGGGCCTCCGGCGACCTCGTCGAGTACTGCTCTCGCCTGATCGCCACCGGCGCCTCCTTCGCGCTCTTCGTGGGCCTCCCCGTCGCCTCCTTCGGCGTCTTCGGGGCCCAATCGGAGCCGCTCAAGCAGTCCCTCTACGCAGTTTCCAGTGGGTTTTTGGTGGTCACTCTCGCCGTGGTGAGGATGTACCTCGGTTGGGCTTACGTCGGCAACCGCTTGCTTAGTGCCACTGTTGAATACGAAGAGACTGGGTGGTATGATGGTCAGATATGGGTCAAGACTGCTGAAGTTTTGGCGCGTGATCGGCTTCTCGGTTCATTTTCTGTGAGTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1230

Protein Analysis

183

Amino Acids

20.04

Weight (kDa)

5.58

Isoelectric Point (pI)

44.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CGLD27-like PF06799 50 - 181 1.6e-48 Conserved in the green lineage and diatoms 27
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 266
AccBSI CCGCTC 1 cut(s) 337
AccII CGCG 2 cut(s) 278, 514
AciI CCGC 6 cut(s) 9, 85, 129, 158, 335, 430
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 34
AcuI CTGAAG 1 cut(s) 522
AcyI GRCGYC 2 cut(s) 267, 312
AfaI GTAC 3 cut(s) 185, 242, 404
AfiI CCNNNNNNNGG 3 cut(s) 216, 396, 412
AgsI TTSAA 1 cut(s) 452
AjuI GAANNNNNNNTTGG 2 cut(s) 83, 115
Alw26I GTCTC 1 cut(s) 455
AoxI GGCC 5 cut(s) 3, 130, 219, 289, 321
ApaI GGGCCC 1 cut(s) 325
ApeKI GCWGC 2 cut(s) 166, 169
ApoI RAATTY 1 cut(s) 34
AspLEI GCGC 3 cut(s) 269, 280, 514
AspS9I GGNCC 5 cut(s) 131, 219, 289, 321, 322
AsuHPI GGTGA 3 cut(s) 36, 68, 406
BaeGI GKGCMC 2 cut(s) 152, 325
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 266
BanII GRGCYC 1 cut(s) 325
BauI CACGAG 1 cut(s) 214
BbsI GAAGAC 1 cut(s) 307
BbvI GCAGC 2 cut(s) 178, 181
BccI CCATC 1 cut(s) 470
BceAI ACGGC 2 cut(s) 142, 374
BcoDI GTCTC 1 cut(s) 455
BfoI RGCGCY 1 cut(s) 270
BisI GCNGC 4 cut(s) 130, 167, 170, 335
BlsI GCNGC 4 cut(s) 131, 168, 171, 336
BmcAI AGTACT 1 cut(s) 242
BmgT120I GGNCC 5 cut(s) 131, 219, 289, 321, 322
BmiI GGNNCC 4 cut(s) 268, 322, 323, 333
BmrI ACTGGG 1 cut(s) 474
BmuI ACTGGG 1 cut(s) 474
BpiI GAAGAC 1 cut(s) 307
BplI GAGNNNNNCTC 2 cut(s) 230, 262
BpmI CTGGAG 1 cut(s) 182
BpuEI CTTGAG 1 cut(s) 323
BsaHI GRCGYC 2 cut(s) 267, 312
BsaJI CCNNGG 2 cut(s) 390, 406
Bsc4I CCNNNNNNNGG 3 cut(s) 216, 396, 412
Bse118I RCCGGY 1 cut(s) 263
Bse1I ACTGG 2 cut(s) 363, 469
BseDI CCNNGG 2 cut(s) 390, 406
BseGI GGATG 1 cut(s) 405
BseLI CCNNNNNNNGG 3 cut(s) 216, 396, 412
BseNI ACTGG 2 cut(s) 363, 469
BseSI GKGCMC 2 cut(s) 152, 325
BseXI GCAGC 2 cut(s) 178, 181
BseYI CCCAGC 1 cut(s) 133
Bsh1236I CGCG 2 cut(s) 278, 514
BshFI GGCC 5 cut(s) 5, 132, 221, 291, 323
BshNI GGYRCC 1 cut(s) 266
BsiSI CCGG 2 cut(s) 225, 264
BslFI GGGAC 1 cut(s) 331
BslI CCNNNNNNNGG 3 cut(s) 216, 396, 412
BsmAI GTCTC 1 cut(s) 455
BsmFI GGGAC 1 cut(s) 331
BsnI GGCC 5 cut(s) 5, 132, 221, 291, 323
Bsp120I GGGCCC 1 cut(s) 321
Bsp1286I GDGCHC 2 cut(s) 152, 325
Bsp143I GATC 2 cut(s) 255, 517
BspACI CCGC 6 cut(s) 9, 85, 129, 158, 335, 430
BspANI GGCC 5 cut(s) 5, 132, 221, 291, 323
BspFNI CGCG 2 cut(s) 278, 514
BspLI GGNNCC 4 cut(s) 268, 322, 323, 333
BspQI GCTCTTC 1 cut(s) 287
BspT107I GGYRCC 1 cut(s) 266
BsrBI CCGCTC 1 cut(s) 337
BsrFI RCCGGY 1 cut(s) 263
BsrI ACTGG 2 cut(s) 363, 469
BssAI RCCGGY 1 cut(s) 263
BssECI CCNNGG 2 cut(s) 390, 406
BssMI GATC 2 cut(s) 255, 517
BssNI GRCGYC 2 cut(s) 267, 312
BssSI CACGAG 1 cut(s) 214
Bst2BI CACGAG 1 cut(s) 214
Bst4CI ACNGT 1 cut(s) 448
Bst6I CTCTTC 2 cut(s) 287, 453
BstACI GRCGYC 2 cut(s) 267, 312
BstC8I GCNNGC 1 cut(s) 434
BstDEI CTNAG 1 cut(s) 437
BstDSI CCRYGG 1 cut(s) 390
BstF5I GGATG 1 cut(s) 405
BstFNI CGCG 2 cut(s) 278, 514
BstH2I RGCGCY 1 cut(s) 270
BstHHI GCGC 3 cut(s) 269, 280, 514
BstKTI GATC 2 cut(s) 258, 520
BstMAI GTCTC 1 cut(s) 455
BstMBI GATC 2 cut(s) 255, 517
BstMWI GCNNNNNNNGC 5 cut(s) 166, 266, 275, 340, 520
BstSLI GKGCMC 2 cut(s) 152, 325
BstUI CGCG 2 cut(s) 278, 514
BstV1I GCAGC 2 cut(s) 178, 181
BstV2I GAAGAC 1 cut(s) 307
BsuRI GGCC 5 cut(s) 5, 132, 221, 291, 323
BtgI CCRYGG 1 cut(s) 390
BtsCI GGATG 1 cut(s) 405
BtsIMutI CAGTG 2 cut(s) 370, 444
Cac8I GCNNGC 1 cut(s) 434
CfoI GCGC 3 cut(s) 269, 280, 514
Cfr10I RCCGGY 1 cut(s) 263
Cfr13I GGNCC 5 cut(s) 131, 219, 289, 321, 322
CseI GACGC 1 cut(s) 301
Csp6I GTAC 3 cut(s) 184, 241, 403
CviJI RGCY 9 cut(s) 5, 132, 172, 221, 291, 323, 334, 416, 523
CviKI_1 RGCY 9 cut(s) 5, 132, 172, 221, 291, 323, 334, 416, 523
CviQI GTAC 3 cut(s) 184, 241, 403
DdeI CTNAG 1 cut(s) 437
DinI GGCGCC 1 cut(s) 268
DpnI GATC 2 cut(s) 257, 519
DpnII GATC 2 cut(s) 255, 517
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 287, 453
EarI CTCTTC 2 cut(s) 287, 453
Eco24I GRGCYC 1 cut(s) 325
Eco57I CTGAAG 1 cut(s) 522
EcoO109I RGGNCCY 1 cut(s) 321
EcoT38I GRGCYC 1 cut(s) 325
EgeI GGCGCC 1 cut(s) 268
EheI GGCGCC 1 cut(s) 268
FaiI YATR 3 cut(s) 176, 474, 487
FaqI GGGAC 1 cut(s) 331
Fnu4HI GCNGC 4 cut(s) 130, 167, 170, 335
FokI GGATG 1 cut(s) 412
FriOI GRGCYC 1 cut(s) 325
Fsp4HI GCNGC 4 cut(s) 130, 167, 170, 335
GlaI GCGC 3 cut(s) 268, 279, 513
GluI GCNGC 4 cut(s) 130, 167, 170, 335
GsaI CCCAGC 1 cut(s) 137
GsuI CTGGAG 1 cut(s) 182
HaeII RGCGCY 1 cut(s) 270
HaeIII GGCC 5 cut(s) 5, 132, 221, 291, 323
HapII CCGG 2 cut(s) 225, 264
HgaI GACGC 1 cut(s) 301
HhaI GCGC 3 cut(s) 269, 280, 514
Hin1I GRCGYC 2 cut(s) 267, 312
Hin6I GCGC 3 cut(s) 267, 278, 512
HinP1I GCGC 3 cut(s) 267, 278, 512
HpaII CCGG 2 cut(s) 225, 264
HphI GGTGA 3 cut(s) 36, 68, 406
Hpy188I TCNGA 3 cut(s) 122, 331, 483
Hpy188III TCNNGA 1 cut(s) 493
Hpy99I CGWCG 7 cut(s) 109, 112, 115, 118, 239, 302, 425
HpyAV CCTTC 5 cut(s) 109, 214, 220, 283, 316
HpyCH4III ACNGT 1 cut(s) 448
HpyCH4IV ACGT 1 cut(s) 420
HpyF10VI GCNNNNNNNGC 5 cut(s) 166, 266, 275, 340, 520
HpyF3I CTNAG 1 cut(s) 437
HpySE526I ACGT 1 cut(s) 420
Hsp92I GRCGYC 2 cut(s) 267, 312
HspAI GCGC 3 cut(s) 267, 278, 512
KasI GGCGCC 1 cut(s) 266
Kzo9I GATC 2 cut(s) 255, 517
LguI GCTCTTC 1 cut(s) 287
LmnI GCTCC 2 cut(s) 163, 331
Lsp1109I GCAGC 2 cut(s) 178, 181
MaeII ACGT 1 cut(s) 420
MaeIII GTNAC 1 cut(s) 379
MalI GATC 2 cut(s) 257, 519
MbiI CCGCTC 1 cut(s) 337
MboI GATC 2 cut(s) 255, 517
MboII GAAGA 4 cut(s) 8, 274, 307, 470
MhlI GDGCHC 2 cut(s) 152, 325
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 34
MluNI TGGCCA 1 cut(s) 5
Mly113I GGCGCC 1 cut(s) 267
MmeI TCCRAC 1 cut(s) 80
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 160
MspI CCGG 2 cut(s) 225, 264
MvnI CGCG 2 cut(s) 278, 514
MwoI GCNNNNNNNGC 5 cut(s) 166, 266, 275, 340, 520
NarI GGCGCC 1 cut(s) 267
NdeII GATC 2 cut(s) 255, 517
NlaIV GGNNCC 4 cut(s) 268, 322, 323, 333
NmuCI GTSAC 1 cut(s) 379
PciSI GCTCTTC 1 cut(s) 287
PcsI WCGNNNNNNNCGW 3 cut(s) 110, 113, 282
PflFI GACNNNGTC 1 cut(s) 233
PkrI GCNGC 4 cut(s) 131, 168, 171, 336
PluTI GGCGCC 1 cut(s) 270
PspFI CCCAGC 1 cut(s) 133
PspN4I GGNNCC 4 cut(s) 268, 322, 323, 333
PspOMI GGGCCC 1 cut(s) 321
PspPI GGNCC 5 cut(s) 131, 219, 289, 321, 322
PsyI GACNNNGTC 1 cut(s) 233
RsaI GTAC 3 cut(s) 185, 242, 404
RsaNI GTAC 3 cut(s) 184, 241, 403
SapI GCTCTTC 1 cut(s) 287
SatI GCNGC 4 cut(s) 130, 167, 170, 335
Sau3AI GATC 2 cut(s) 255, 517
Sau96I GGNCC 5 cut(s) 131, 219, 289, 321, 322
ScaI AGTACT 1 cut(s) 242
SduI GDGCHC 2 cut(s) 152, 325
SetI ASST 5 cut(s) 195, 203, 234, 408, 423
SfoI GGCGCC 1 cut(s) 268
SgrAI CRCCGGYG 1 cut(s) 263
SmlI CTYRAG 1 cut(s) 338
SmoI CTYRAG 1 cut(s) 338
Sse9I AATT 1 cut(s) 34
SsiI CCGC 6 cut(s) 9, 85, 129, 158, 335, 430
SspDI GGCGCC 1 cut(s) 266
TaaI ACNGT 1 cut(s) 448
TaiI ACGT 1 cut(s) 423
TaqI TCGA 1 cut(s) 237
TasI AATT 1 cut(s) 34
TatI WGTACW 1 cut(s) 240
TauI GCSGC 2 cut(s) 132, 337
TscAI CASTG 2 cut(s) 370, 451
TseFI GTSAC 1 cut(s) 379
TseI GCWGC 2 cut(s) 166, 169
Tsp45I GTSAC 1 cut(s) 379
TspDTI ATGAA 1 cut(s) 522
TspRI CASTG 2 cut(s) 370, 451
Tth111I GACNNNGTC 1 cut(s) 233
XapI RAATTY 1 cut(s) 34
XcmI CCANNNNNNNNNTGG 1 cut(s) 370
ZrmI AGTACT 1 cut(s) 242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.