RLG00000010455

Protein of unknown function (DUF1230)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0008080
Physical Location & Seq
Forward (+)
1746209 .. 1750000
3792 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010455

Sequence Viewer

Length: 1077 bp
ATGGCCACCGCACTTCTCCTACACCCAACCCCAAATTTCCTCTCACCAATCCCTCCAACCCCAAATCCCAGACTGCACCACCACCGCTACCGCACCAAAATACCCAAAATACCCTTCTCCTCGTCGTCGTTCCGAAACGGCGGCCCAGCAGAAACAGAGTGCCCTGTTCCGCTGGAGCAGCAGCCCATAAACGAGTACCAGAACCTCTCCACCTCCTTCCCCTTCTCCTGGGCCTCCGGCGACCTTGTCGAGTACTGCTCTCGCCTGATCGCCACCGGCGCCTCCTTCGCGCTCTTCGTGGGCCTCCCCGTCGCCTCCTTCGGCGTCTTCAGGGCCCAATCGGAGCCGCTCAAGCAGTCCCTCTACGCAGTTTCCAGTGGGTTTTTGGTGGTCACTCTCGCCGTGGTGAGGATGTACCTCGGTTGGGCTTACGTCGGAAACCGCTTGCTTAGTGCCACTGTTGAATACGAAGAGACTGGGTGGTATGATGGTCAGATATGGGTTAAGACTGCTGAAGTTTTGGCGCGTGATCGGCTTCTTGGTTCATTTTCTGTGAAGCCTGTGCTGAGCAGATTGAAGTTAACGCTGGTGACTCTTGCAGCATCGATACTTGCATGTGTTCTCGTCCTCCTTTACACTGATGGAGGCCAAATCCAAGAAGCCGAGAATCGCGTTATACGTGGAGTTTACAACGATGATTCTGCAAGATCGTTTGAGCCCGATGCGTTTTGTGGTGGTGATCCTGGATCAATTCGATGTTCATGGTTGGTGGTGTTGGAACCACAAGGAAGAGAGAAATGCAATCTAAGTGGTGGTGGAGCTCGAGGTGGTTGGAGGAGAAAGAATCCGGTTGCAACCCGTTTTGATTCTCCTATTCAATACATCGCTGTGGGTGGTTATGGAGGATGGTGGAGTTTAGGCAGCAGCTACACGGGAGGAGGCGGTCATTTTGAGACCGGTTCCATGGCTGGAGGGGTTGTTCTTCTCATATTCTGCCCAGCTATCACCAATCCTTGCAGTGTGAGTCACGTTGTGATGTTTTTCCAGAAAAGATTAGAGAAAGCAAATGCATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1230

Protein Analysis

359

Amino Acids

38.92

Weight (kDa)

8.74

Isoelectric Point (pI)

38.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CGLD27-like PF06799 54 - 197 9.1e-54 Conserved in the green lineage and diatoms 27
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 278
AccBSI CCGCTC 1 cut(s) 349
AccII CGCG 3 cut(s) 290, 526, 672
AciI CCGC 8 cut(s) 9, 85, 91, 141, 170, 347, 442, 942
AclWI GGATC 2 cut(s) 734, 754
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 34
AcuI CTGAAG 2 cut(s) 313, 534
AcyI GRCGYC 2 cut(s) 279, 324
AdeI CACNNNGTG 1 cut(s) 1033
AfaI GTAC 3 cut(s) 197, 254, 416
AfiI CCNNNNNNNGG 3 cut(s) 228, 408, 424
AgeI ACCGGT 1 cut(s) 956
AgsI TTSAA 3 cut(s) 464, 577, 878
AjnI CCWGG 2 cut(s) 227, 742
AjuI GAANNNNNNNTTGG 2 cut(s) 98, 130
AloI GAACNNNNNNTCC 2 cut(s) 963, 995
AluBI AGCT 3 cut(s) 821, 927, 1001
AluI AGCT 3 cut(s) 821, 927, 1001
Alw21I GWGCWC 1 cut(s) 823
Alw26I GTCTC 2 cut(s) 467, 947
AlwI GGATC 2 cut(s) 734, 754
Ama87I CYCGRG 1 cut(s) 822
AoxI GGCC 6 cut(s) 3, 142, 231, 301, 333, 646
ApaI GGGCCC 1 cut(s) 337
ApeKI GCWGC 5 cut(s) 178, 181, 599, 921, 924
ApoI RAATTY 1 cut(s) 34
AsiGI ACCGGT 1 cut(s) 956
AspLEI GCGC 3 cut(s) 281, 292, 526
AspS9I GGNCC 5 cut(s) 143, 231, 301, 333, 334
AsuHPI GGTGA 5 cut(s) 36, 418, 601, 749, 997
AvaI CYCGRG 1 cut(s) 822
BaeGI GKGCMC 2 cut(s) 164, 337
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 278
BanII GRGCYC 3 cut(s) 337, 720, 823
BbsI GAAGAC 1 cut(s) 319
Bbv12I GWGCWC 1 cut(s) 823
BbvI GCAGC 5 cut(s) 190, 193, 611, 933, 936
BccI CCATC 3 cut(s) 482, 635, 900
BceAI ACGGC 2 cut(s) 154, 386
BcgI CGANNNNNNTGC 2 cut(s) 683, 717
BciT130I CCWGG 2 cut(s) 229, 744
BcoDI GTCTC 2 cut(s) 467, 947
BfoI RGCGCY 1 cut(s) 282
BisI GCNGC 7 cut(s) 142, 179, 182, 347, 600, 922, 925
BlpI GCTNAGC 1 cut(s) 566
BlsI GCNGC 7 cut(s) 143, 180, 183, 348, 601, 923, 926
BmcAI AGTACT 1 cut(s) 254
Bme1390I CCNGG 2 cut(s) 229, 744
BmeT110I CYCGRG 1 cut(s) 822
BmgT120I GGNCC 5 cut(s) 143, 231, 301, 333, 334
BmiI GGNNCC 5 cut(s) 280, 335, 345, 780, 961
BmrFI CCNGG 2 cut(s) 229, 744
BmrI ACTGGG 1 cut(s) 486
BmsI GCATC 2 cut(s) 611, 712
BmuI ACTGGG 1 cut(s) 486
BpiI GAAGAC 1 cut(s) 319
BplI GAGNNNNNCTC 2 cut(s) 242, 274
BpmI CTGGAG 2 cut(s) 194, 990
Bpu1102I GCTNAGC 1 cut(s) 566
BpuEI CTTGAG 1 cut(s) 335
Bsa29I ATCGAT 1 cut(s) 605
BsaAI YACGTR 1 cut(s) 680
BsaHI GRCGYC 2 cut(s) 279, 324
BsaI GGTCTC 1 cut(s) 947
BsaJI CCNNGG 4 cut(s) 228, 402, 418, 963
BsaWI WCCGGW 2 cut(s) 847, 956
BsaXI ACNNNNNCTCC 2 cut(s) 963, 993
Bsc4I CCNNNNNNNGG 3 cut(s) 228, 408, 424
Bse118I RCCGGY 2 cut(s) 275, 956
Bse1I ACTGG 2 cut(s) 375, 481
BseBI CCWGG 2 cut(s) 229, 744
BseCI ATCGAT 1 cut(s) 605
BseDI CCNNGG 4 cut(s) 228, 402, 418, 963
BseGI GGATG 2 cut(s) 417, 911
BseLI CCNNNNNNNGG 3 cut(s) 228, 408, 424
BseMII CTCAG 1 cut(s) 557
BseNI ACTGG 2 cut(s) 375, 481
BseRI GAGGAG 3 cut(s) 109, 850, 951
BseSI GKGCMC 2 cut(s) 164, 337
BseXI GCAGC 5 cut(s) 190, 193, 611, 933, 936
BseYI CCCAGC 2 cut(s) 145, 997
BsgI GTGCAG 1 cut(s) 59
Bsh1236I CGCG 3 cut(s) 290, 526, 672
BshFI GGCC 6 cut(s) 5, 144, 233, 303, 335, 648
BshNI GGYRCC 1 cut(s) 278
BshTI ACCGGT 1 cut(s) 956
BshVI ATCGAT 1 cut(s) 605
BsiHKAI GWGCWC 1 cut(s) 823
BsiHKCI CYCGRG 1 cut(s) 822
BsiSI CCGG 4 cut(s) 237, 276, 848, 957
BslFI GGGAC 1 cut(s) 343
BslI CCNNNNNNNGG 3 cut(s) 228, 408, 424
BsmAI GTCTC 2 cut(s) 467, 947
BsmFI GGGAC 1 cut(s) 343
BsnI GGCC 6 cut(s) 5, 144, 233, 303, 335, 648
Bso31I GGTCTC 1 cut(s) 947
BsoBI CYCGRG 1 cut(s) 822
Bsp120I GGGCCC 1 cut(s) 333
Bsp1286I GDGCHC 4 cut(s) 164, 337, 720, 823
Bsp143I GATC 5 cut(s) 267, 529, 707, 739, 746
Bsp1720I GCTNAGC 1 cut(s) 566
Bsp19I CCATGG 1 cut(s) 963
BspACI CCGC 8 cut(s) 9, 85, 91, 141, 170, 347, 442, 942
BspANI GGCC 6 cut(s) 5, 144, 233, 303, 335, 648
BspCNI CTCAG 1 cut(s) 558
BspDI ATCGAT 1 cut(s) 605
BspFNI CGCG 3 cut(s) 290, 526, 672
BspLI GGNNCC 5 cut(s) 280, 335, 345, 780, 961
BspPI GGATC 2 cut(s) 734, 754
BspQI GCTCTTC 1 cut(s) 299
BspT107I GGYRCC 1 cut(s) 278
BspTNI GGTCTC 1 cut(s) 947
BsrBI CCGCTC 1 cut(s) 349
BsrFI RCCGGY 2 cut(s) 275, 956
BsrI ACTGG 2 cut(s) 375, 481
BssAI RCCGGY 2 cut(s) 275, 956
BssECI CCNNGG 4 cut(s) 228, 402, 418, 963
BssMI GATC 5 cut(s) 267, 529, 707, 739, 746
BssNI GRCGYC 2 cut(s) 279, 324
BssT1I CCWWGG 1 cut(s) 963
Bst2UI CCWGG 2 cut(s) 229, 744
Bst4CI ACNGT 1 cut(s) 460
Bst6I CTCTTC 3 cut(s) 299, 465, 784
BstACI GRCGYC 2 cut(s) 279, 324
BstBAI YACGTR 1 cut(s) 680
BstC8I GCNNGC 1 cut(s) 446
BstDEI CTNAG 3 cut(s) 449, 566, 806
BstDSI CCRYGG 2 cut(s) 402, 963
BstF5I GGATG 2 cut(s) 417, 911
BstFNI CGCG 3 cut(s) 290, 526, 672
BstH2I RGCGCY 1 cut(s) 282
BstHHI GCGC 3 cut(s) 281, 292, 526
BstKTI GATC 5 cut(s) 270, 532, 710, 742, 749
BstMAI GTCTC 2 cut(s) 467, 947
BstMBI GATC 5 cut(s) 267, 529, 707, 739, 746
BstMWI GCNNNNNNNGC 5 cut(s) 178, 278, 287, 352, 532
BstNI CCWGG 2 cut(s) 229, 744
BstNSI RCATGY 1 cut(s) 618
BstSCI CCNGG 2 cut(s) 227, 742
BstSLI GKGCMC 2 cut(s) 164, 337
BstUI CGCG 3 cut(s) 290, 526, 672
BstV1I GCAGC 5 cut(s) 190, 193, 611, 933, 936
BstV2I GAAGAC 1 cut(s) 319
Bsu15I ATCGAT 1 cut(s) 605
BsuRI GGCC 6 cut(s) 5, 144, 233, 303, 335, 648
BsuTUI ATCGAT 1 cut(s) 605
BtgI CCRYGG 2 cut(s) 402, 963
BtgZI GCGATG 1 cut(s) 868
BtsCI GGATG 2 cut(s) 417, 911
BtsI GCAGTG 1 cut(s) 1024
BtsIMutI CAGTG 4 cut(s) 382, 456, 636, 1024
Cac8I GCNNGC 1 cut(s) 446
CfoI GCGC 3 cut(s) 281, 292, 526
Cfr10I RCCGGY 2 cut(s) 275, 956
Cfr13I GGNCC 5 cut(s) 143, 231, 301, 333, 334
ClaI ATCGAT 1 cut(s) 605
CseI GACGC 1 cut(s) 313
Csp6I GTAC 3 cut(s) 196, 253, 415
CspAI ACCGGT 1 cut(s) 956
CspCI CAANNNNNGTGG 2 cut(s) 790, 825
CviAII CATG 3 cut(s) 615, 762, 964
CviQI GTAC 3 cut(s) 196, 253, 415
DdeI CTNAG 3 cut(s) 449, 566, 806
DinI GGCGCC 1 cut(s) 280
DpnI GATC 5 cut(s) 269, 531, 709, 741, 748
DpnII GATC 5 cut(s) 267, 529, 707, 739, 746
DraIII CACNNNGTG 1 cut(s) 1033
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 299, 465, 784
EarI CTCTTC 3 cut(s) 299, 465, 784
Ecl136II GAGCTC 1 cut(s) 821
Eco130I CCWWGG 1 cut(s) 963
Eco24I GRGCYC 3 cut(s) 337, 720, 823
Eco31I GGTCTC 1 cut(s) 947
Eco53kI GAGCTC 1 cut(s) 821
Eco57I CTGAAG 2 cut(s) 313, 534
Eco88I CYCGRG 1 cut(s) 822
EcoICRI GAGCTC 1 cut(s) 821
EcoO109I RGGNCCY 1 cut(s) 333
EcoRII CCWGG 2 cut(s) 227, 742
EcoT14I CCWWGG 1 cut(s) 963
EcoT22I ATGCAT 1 cut(s) 1072
EcoT38I GRGCYC 3 cut(s) 337, 720, 823
EgeI GGCGCC 1 cut(s) 280
EheI GGCGCC 1 cut(s) 280
ErhI CCWWGG 1 cut(s) 963
FaeI CATG 3 cut(s) 618, 765, 967
FaqI GGGAC 1 cut(s) 343
FatI CATG 3 cut(s) 614, 761, 963
Fnu4HI GCNGC 7 cut(s) 142, 179, 182, 347, 600, 922, 925
FokI GGATG 2 cut(s) 424, 918
FriOI GRGCYC 3 cut(s) 337, 720, 823
Fsp4HI GCNGC 7 cut(s) 142, 179, 182, 347, 600, 922, 925
GlaI GCGC 3 cut(s) 280, 291, 525
GluI GCNGC 7 cut(s) 142, 179, 182, 347, 600, 922, 925
GsaI CCCAGC 2 cut(s) 149, 1001
GsuI CTGGAG 2 cut(s) 194, 990
HaeII RGCGCY 1 cut(s) 282
HaeIII GGCC 6 cut(s) 5, 144, 233, 303, 335, 648
HapII CCGG 4 cut(s) 237, 276, 848, 957
HgaI GACGC 1 cut(s) 313
HhaI GCGC 3 cut(s) 281, 292, 526
Hin1I GRCGYC 2 cut(s) 279, 324
Hin1II CATG 3 cut(s) 618, 765, 967
Hin6I GCGC 3 cut(s) 279, 290, 524
HinP1I GCGC 3 cut(s) 279, 290, 524
HincII GTYRAC 1 cut(s) 582
HindII GTYRAC 1 cut(s) 582
HinfI GANTC 6 cut(s) 592, 667, 698, 844, 866, 1024
HpaI GTTAAC 1 cut(s) 582
HpaII CCGG 4 cut(s) 237, 276, 848, 957
HphI GGTGA 5 cut(s) 36, 418, 601, 749, 997
Hpy166II GTNNAC 2 cut(s) 582, 688
Hpy188I TCNGA 4 cut(s) 134, 343, 437, 495
Hpy188III TCNNGA 1 cut(s) 1045
Hpy8I GTNNAC 2 cut(s) 582, 688
Hpy99I CGWCG 4 cut(s) 127, 130, 314, 437
HpyAV CCTTC 5 cut(s) 124, 226, 232, 295, 328
HpyCH4III ACNGT 1 cut(s) 460
HpyCH4IV ACGT 3 cut(s) 432, 679, 1029
HpyCH4V TGCA 8 cut(s) 76, 599, 614, 704, 801, 854, 1017, 1070
HpyF10VI GCNNNNNNNGC 5 cut(s) 178, 278, 287, 352, 532
HpyF3I CTNAG 3 cut(s) 449, 566, 806
HpySE526I ACGT 3 cut(s) 432, 679, 1029
Hsp92I GRCGYC 2 cut(s) 279, 324
Hsp92II CATG 3 cut(s) 618, 765, 967
HspAI GCGC 3 cut(s) 279, 290, 524
KasI GGCGCC 1 cut(s) 278
KspAI GTTAAC 1 cut(s) 582
Kzo9I GATC 5 cut(s) 267, 529, 707, 739, 746
LguI GCTCTTC 1 cut(s) 299
LmnI GCTCC 3 cut(s) 175, 343, 818
Lsp1109I GCAGC 5 cut(s) 190, 193, 611, 933, 936
LweI GCATC 2 cut(s) 611, 712
MaeII ACGT 3 cut(s) 432, 679, 1029
MaeIII GTNAC 3 cut(s) 391, 589, 1025
MalI GATC 5 cut(s) 269, 531, 709, 741, 748
MbiI CCGCTC 1 cut(s) 349
MboI GATC 5 cut(s) 267, 529, 707, 739, 746
MboII GAAGA 5 cut(s) 286, 319, 482, 801, 974
MhlI GDGCHC 4 cut(s) 164, 337, 720, 823
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 34, 750
MluNI TGGCCA 1 cut(s) 5
Mly113I GGCGCC 1 cut(s) 279
MlyI GAGTC 2 cut(s) 586, 1033
MmeI TCCRAC 4 cut(s) 80, 415, 756, 812
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 1072
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 504, 581
MslI CAYNNNNRTG 1 cut(s) 887
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 172
MspI CCGG 4 cut(s) 237, 276, 848, 957
MspR9I CCNGG 2 cut(s) 229, 744
MvaI CCWGG 2 cut(s) 229, 744
MvnI CGCG 3 cut(s) 290, 526, 672
MwoI GCNNNNNNNGC 5 cut(s) 178, 278, 287, 352, 532
NarI GGCGCC 1 cut(s) 279
NcoI CCATGG 1 cut(s) 963
NdeII GATC 5 cut(s) 267, 529, 707, 739, 746
NlaIII CATG 3 cut(s) 618, 765, 967
NlaIV GGNNCC 5 cut(s) 280, 335, 345, 780, 961
NmeAIII GCCGAG 1 cut(s) 688
NmuCI GTSAC 3 cut(s) 391, 589, 1025
NsiI ATGCAT 1 cut(s) 1072
NspI RCATGY 1 cut(s) 618
PaeR7I CTCGAG 1 cut(s) 822
PciSI GCTCTTC 1 cut(s) 299
PcsI WCGNNNNNNNCGW 2 cut(s) 294, 676
PfeI GAWTC 4 cut(s) 667, 698, 844, 866
PflFI GACNNNGTC 1 cut(s) 245
PfoI TCCNGGA 1 cut(s) 742
PinAI ACCGGT 1 cut(s) 956
PkrI GCNGC 7 cut(s) 143, 180, 183, 348, 601, 923, 926
PleI GAGTC 2 cut(s) 586, 1032
PluTI GGCGCC 1 cut(s) 282
PpsI GAGTC 2 cut(s) 586, 1032
Ppu21I YACGTR 1 cut(s) 680
Psp124BI GAGCTC 1 cut(s) 823
Psp6I CCWGG 2 cut(s) 227, 742
PspFI CCCAGC 2 cut(s) 145, 997
PspGI CCWGG 2 cut(s) 227, 742
PspN4I GGNNCC 5 cut(s) 280, 335, 345, 780, 961
PspOMI GGGCCC 1 cut(s) 333
PspPI GGNCC 5 cut(s) 143, 231, 301, 333, 334
PspXI VCTCGAGB 1 cut(s) 822
PsyI GACNNNGTC 1 cut(s) 245
RsaI GTAC 3 cut(s) 197, 254, 416
RsaNI GTAC 3 cut(s) 196, 253, 415
RseI CAYNNNNRTG 1 cut(s) 887
SacI GAGCTC 1 cut(s) 823
SapI GCTCTTC 1 cut(s) 299
SaqAI TTAA 2 cut(s) 504, 581
SatI GCNGC 7 cut(s) 142, 179, 182, 347, 600, 922, 925
Sau3AI GATC 5 cut(s) 267, 529, 707, 739, 746
Sau96I GGNCC 5 cut(s) 143, 231, 301, 333, 334
ScaI AGTACT 1 cut(s) 254
SchI GAGTC 2 cut(s) 586, 1033
ScrFI CCNGG 2 cut(s) 229, 744
SduI GDGCHC 4 cut(s) 164, 337, 720, 823
SfaNI GCATC 2 cut(s) 611, 712
SfoI GGCGCC 1 cut(s) 280
Sfr274I CTCGAG 1 cut(s) 822
SgrAI CRCCGGYG 1 cut(s) 275
SlaI CTCGAG 1 cut(s) 822
SmiMI CAYNNNNRTG 1 cut(s) 887
SmlI CTYRAG 2 cut(s) 350, 822
SmoI CTYRAG 2 cut(s) 350, 822
Sse9I AATT 2 cut(s) 34, 750
SsiI CCGC 8 cut(s) 9, 85, 91, 141, 170, 347, 442, 942
SspDI GGCGCC 1 cut(s) 278
SstI GAGCTC 1 cut(s) 823
StyD4I CCNGG 2 cut(s) 227, 742
StyI CCWWGG 1 cut(s) 963
TaaI ACNGT 1 cut(s) 460
TaiI ACGT 3 cut(s) 435, 682, 1032
TaqI TCGA 4 cut(s) 249, 605, 754, 823
TasI AATT 2 cut(s) 34, 750
TatI WGTACW 1 cut(s) 252
TauI GCSGC 2 cut(s) 144, 349
TfiI GAWTC 4 cut(s) 667, 698, 844, 866
Tru1I TTAA 2 cut(s) 504, 581
Tru9I TTAA 2 cut(s) 504, 581
TscAI CASTG 4 cut(s) 382, 463, 643, 1024
TseFI GTSAC 3 cut(s) 391, 589, 1025
TseI GCWGC 5 cut(s) 178, 181, 599, 921, 924
Tsp45I GTSAC 3 cut(s) 391, 589, 1025
TspDTI ATGAA 2 cut(s) 534, 750
TspRI CASTG 4 cut(s) 382, 463, 643, 1024
Tth111I GACNNNGTC 1 cut(s) 245
XapI RAATTY 1 cut(s) 34
XceI RCATGY 1 cut(s) 618
XcmI CCANNNNNNNNNTGG 1 cut(s) 382
XhoI CTCGAG 1 cut(s) 822
ZrmI AGTACT 1 cut(s) 254
Zsp2I ATGCAT 1 cut(s) 1072
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.