FvH4_2g32190

Vacuolar-processing enzyme-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0009785
Physical Location & Seq
Forward (+)
24489839 .. 24493656
3818 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g32190.t4

Sequence Viewer

Length: 1539 bp
ATGACTCGCTTGGCCGCCGTCGTCGCCGTCGTCCTTTTCTTCCTTGTCTCCCTTTTTTCTTCCTCCACCAGCGCCCGCGACCTCCCCGGAGACGTCTTACGCTTGCCGTCGGAGACTTCGAGATTCTTCCGCGCCGGCGACGATCAGCAAGACGACGGCACCGTCGGCACGCGGTGGGCCGTTTTGATCGCCGGCTCCAATGGATACTGGAATTACAGGCATCAGGCGGATATATGCCATGCGTATCAGTTATTGAAGAAAGGAGGACTGAAAGATGAGAATATCGTTGTGTTTATGTATGATGATATTGCTTACAATGAAGAGAACCCTAGGCAAGGAGTCATCATCAACAGTCCTCATGGTGATGATGTTTATAAAGGAGTCCCCAAGGATTACACTGGGGAAGATGTTACTGTTGGTAACTTCTTTGCCGCCATCCTTGGAAACAAAACTGCTATCTCCGGGGGTAGTGGGAAAGTTGTGGACAGCGGTCCGAATGATCATATCTTCATATACTATTCCGATCATGGCGGTCCTGGAGTACTCGGGGAAAAACATAATATTGTAGTGACTGTAGCCTTCTATGGAATTCGAGAAATAGGGATGCCTACCAGTCCTTATATTTATGCCGACCGCCTCATAGAAGTCCTAAAGAAGAAGCATGCAGCAGGGACATATGAAAGCTTGGTATTTTATCTTGAAGCTTGTGAATCCGGAAGTATCTTTGAGGGTCTTCTTCCTGAAGGTTTGAATATCTTTGCAACTACAGCATCAAATGCTGAAGAGAGCAGTTGGGGGACTTACTGCCCGGGAGAGTATCCTAGTCCTCCCCCAGAATATGAAACCTGTTTGGGTGACTTGTATAGTGTTGCTTGGATGGAAGATAGTGACATTCACAACTTGAGGTCAGAAACTTTGCACCAGCAGTATGAACTGGTCAAATCAAGGACTGCAAGTGATAATTCTCCTTATGGTTCTCATGTTATGCAATATGGTGATATACCTCTTAGCAAGAACAACCTATTCATGTATATGGGTACAAATCCTGCAAATGATAACTACACCTTCATGCCTCAGAACTTCTTGAGGCCATCTTCTTCAAAAGCTGTCAACCAGAGGGATGCTGATCTTGTCCATTTTTGGCATAAGTACCGCAAGGCCCCTGAAGGCTCAGCAAGGAAAGCTCAAGCTCAAAAAGAATTTCTTGAAGCAATGTCTCACAGAATGCATATAGATGAAAGCGTGAAACTTATCGGTAAACTCTTGTTTGGAATCAAAAAAGGTCCAGAAGTCCTCAGCGCTGTACGACCTGCCGGGCAGCCACTTGTTGATGACTGGGACTGCCTTAAGACAATGGTGAGGAGTTTTGAGACATATTGCGGATCACTTTCTCAGTATGGGATGAAACACATGCGGTCCCTAGCAAACATCTGCAATGCTGGAATGACAAAGGAGCAGATGGCCGAGGCTTCAGCACAAGCCTGTGTCAATGTTCCTTCTGGCCGTTGGAGCTCTCTGCACAGGGGATTCAGTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04612 map04142 map04612
Pfam Domains
Protein Families
Peptidase_C13

Protein Analysis

513

Amino Acids

56.68

Weight (kDa)

5.75

Isoelectric Point (pI)

47.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C13 PF01650 58 - 329 9.9e-118 Peptidase C13 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009785)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G25940 AT4G32940
fragaria_vesca FvH4_2g32190 FvH4_2g32190 FvH4_2g32190 FvH4_2g32190
malus_domestica MD08G1073500.v1.1
prunus_persica Prupe.1G415000_v2.0.a1 Prupe.1G415000_v2.0.a1
pyrus_communis pycom08g06090 pycom15g05790
rosa_chinensis RchiOBHm_Chr6g0310481
rosa_laevigata RLG00000010479
rosa_multiflora Rmu_sc0035020.1_g000006
rosa_roxburghii Rroxscaffold_7G00158490
rosa_rugosa Rorug06G0380700
rosa_samantha Rh6AG494700 Rh6BG504400 Rh6CG509600 Rh6DG495600
rosa_wichuraiana Rw6G043030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 375
AasI GACNNNNNNGTC 1 cut(s) 161
AatII GACGTC 1 cut(s) 96
Acc36I ACCTGC 1 cut(s) 1318
AccB1I GGYRCC 1 cut(s) 158
AccII CGCG 3 cut(s) 78, 132, 172
AccIII TCCGGA 1 cut(s) 713
AclWI GGATC 1 cut(s) 1390
AcoI YGGCCR 3 cut(s) 12, 1461, 1501
AcsI RAATTY 2 cut(s) 588, 1199
AcuI CTGAAG 4 cut(s) 762, 801, 1185, 1455
AcyI GRCGYC 1 cut(s) 93
AdeI CACNNNGTG 1 cut(s) 174
AfaI GTAC 4 cut(s) 543, 1039, 1151, 1305
AfeI AGCGCT 1 cut(s) 1300
AfiI CCNNNNNNNGG 1 cut(s) 335
AflII CTTAAG 1 cut(s) 1346
AgsI TTSAA 5 cut(s) 256, 701, 751, 1101, 1208
AjnI CCWGG 1 cut(s) 535
AluBI AGCT 6 cut(s) 684, 704, 1106, 1184, 1190, 1512
AluI AGCT 6 cut(s) 684, 704, 1106, 1184, 1190, 1512
Alw21I GWGCWC 1 cut(s) 1514
Alw26I GTCTC 5 cut(s) 52, 84, 107, 1221, 1364
AlwI GGATC 1 cut(s) 1390
Ama87I CYCGRG 2 cut(s) 545, 808
Aor13HI TCCGGA 1 cut(s) 713
Aor51HI AGCGCT 1 cut(s) 1300
AoxI GGCC 6 cut(s) 12, 177, 1088, 1158, 1461, 1501
ApeKI GCWGC 2 cut(s) 665, 1318
ApoI RAATTY 2 cut(s) 588, 1199
AspA2I CCTAGG 1 cut(s) 329
AspLEI GCGC 3 cut(s) 74, 134, 1301
AspS9I GGNCC 6 cut(s) 177, 491, 533, 1159, 1283, 1416
AsuC2I CCSGG 5 cut(s) 87, 463, 809, 810, 1315
AsuHPI GGTGA 4 cut(s) 374, 866, 1007, 1369
AvaI CYCGRG 2 cut(s) 545, 808
AvaII GGWCC 4 cut(s) 491, 533, 1283, 1416
AvrII CCTAGG 1 cut(s) 329
BanI GGYRCC 1 cut(s) 158
BanII GRGCYC 1 cut(s) 1514
BbsI GAAGAC 1 cut(s) 725
Bbv12I GWGCWC 1 cut(s) 1514
BbvCI CCTCAGC 1 cut(s) 1295
BbvI GCAGC 2 cut(s) 677, 1330
BccI CCATC 4 cut(s) 443, 871, 1099, 1453
BceAI ACGGC 6 cut(s) 2, 11, 91, 164, 172, 1488
BciT130I CCWGG 1 cut(s) 537
BciVI GTATCC 2 cut(s) 197, 828
BclI TGATCA 1 cut(s) 499
BcnI CCSGG 5 cut(s) 87, 463, 809, 810, 1315
BcoDI GTCTC 5 cut(s) 52, 84, 107, 1221, 1364
BfaI CTAG 3 cut(s) 330, 822, 1421
BfmI CTRYAG 2 cut(s) 573, 765
BfoI RGCGCY 2 cut(s) 75, 1302
BfrI CTTAAG 1 cut(s) 1346
BfuAI ACCTGC 1 cut(s) 1318
BfuI GTATCC 2 cut(s) 197, 828
BisI GCNGC 4 cut(s) 15, 432, 666, 1319
BlnI CCTAGG 1 cut(s) 329
BlpI GCTNAGC 1 cut(s) 1171
BlsI GCNGC 4 cut(s) 16, 433, 667, 1320
BmcAI AGTACT 1 cut(s) 543
Bme1390I CCNGG 6 cut(s) 87, 463, 537, 809, 810, 1315
Bme18I GGWCC 4 cut(s) 491, 533, 1283, 1416
BmeT110I CYCGRG 2 cut(s) 545, 808
BmgT120I GGNCC 6 cut(s) 177, 491, 533, 1159, 1283, 1416
BmiI GGNNCC 4 cut(s) 160, 196, 1161, 1418
BmrFI CCNGG 6 cut(s) 87, 463, 537, 809, 810, 1315
BmrI ACTGGG 2 cut(s) 408, 1345
BmsI GCATC 4 cut(s) 229, 594, 779, 1111
BmuI ACTGGG 2 cut(s) 408, 1345
BoxI GACNNNNGTC 1 cut(s) 489
BpiI GAAGAC 1 cut(s) 725
BpmI CTGGAG 1 cut(s) 558
Bpu10I CCTNAGC 1 cut(s) 1295
Bpu1102I GCTNAGC 1 cut(s) 1171
BpuEI CTTGAG 3 cut(s) 922, 1105, 1170
BpuMI CCSGG 5 cut(s) 87, 463, 809, 810, 1315
BsaBI GATNNNNATC 1 cut(s) 1125
BsaHI GRCGYC 1 cut(s) 93
BsaJI CCNNGG 7 cut(s) 85, 329, 387, 439, 462, 808, 1464
BsaWI WCCGGW 1 cut(s) 713
Bsc4I CCNNNNNNNGG 1 cut(s) 335
Bse118I RCCGGY 2 cut(s) 134, 191
Bse1I ACTGG 5 cut(s) 212, 403, 612, 939, 1340
Bse3DI GCAATG 2 cut(s) 1218, 1441
Bse8I GATNNNNATC 1 cut(s) 1125
BseAI TCCGGA 1 cut(s) 713
BseBI CCWGG 1 cut(s) 537
BseDI CCNNGG 7 cut(s) 85, 329, 387, 439, 462, 808, 1464
BseGI GGATG 5 cut(s) 435, 609, 882, 1126, 1407
BseJI GATNNNNATC 1 cut(s) 1125
BseLI CCNNNNNNNGG 1 cut(s) 335
BseMI GCAATG 2 cut(s) 1218, 1441
BseMII CTCAG 4 cut(s) 1088, 1185, 1309, 1406
BseNI ACTGG 5 cut(s) 212, 403, 612, 939, 1340
BseRI GAGGAG 1 cut(s) 1375
BseXI GCAGC 2 cut(s) 677, 1330
BsgI GTGCAG 1 cut(s) 1502
Bsh1236I CGCG 3 cut(s) 78, 132, 172
Bsh1285I CGRYCG 1 cut(s) 634
BshFI GGCC 6 cut(s) 14, 179, 1090, 1160, 1463, 1503
BshNI GGYRCC 1 cut(s) 158
BsiEI CGRYCG 1 cut(s) 634
BsiHKAI GWGCWC 1 cut(s) 1514
BsiHKCI CYCGRG 2 cut(s) 545, 808
BsiSI CCGG 7 cut(s) 87, 135, 192, 462, 714, 809, 1314
BslFI GGGAC 5 cut(s) 368, 685, 811, 1352, 1402
BslI CCNNNNNNNGG 1 cut(s) 335
BsmAI GTCTC 5 cut(s) 52, 84, 107, 1221, 1364
BsmBI CGTCTC 1 cut(s) 84
BsmFI GGGAC 5 cut(s) 368, 685, 811, 1352, 1402
BsmI GAATGC 1 cut(s) 1230
BsnI GGCC 6 cut(s) 14, 179, 1090, 1160, 1463, 1503
BsoBI CYCGRG 2 cut(s) 545, 808
Bsp1286I GDGCHC 1 cut(s) 1514
Bsp13I TCCGGA 1 cut(s) 713
Bsp143I GATC 6 cut(s) 142, 186, 499, 523, 1126, 1382
Bsp1720I GCTNAGC 1 cut(s) 1171
BspANI GGCC 6 cut(s) 14, 179, 1090, 1160, 1463, 1503
BspCNI CTCAG 4 cut(s) 1087, 1184, 1308, 1405
BspEI TCCGGA 1 cut(s) 713
BspFNI CGCG 3 cut(s) 78, 132, 172
BspLI GGNNCC 4 cut(s) 160, 196, 1161, 1418
BspMI ACCTGC 1 cut(s) 1318
BspPI GGATC 1 cut(s) 1390
BspT107I GGYRCC 1 cut(s) 158
BspTI CTTAAG 1 cut(s) 1346
BsrDI GCAATG 2 cut(s) 1218, 1441
BsrFI RCCGGY 2 cut(s) 134, 191
BsrI ACTGG 5 cut(s) 212, 403, 612, 939, 1340
BssAI RCCGGY 2 cut(s) 134, 191
BssECI CCNNGG 7 cut(s) 85, 329, 387, 439, 462, 808, 1464
BssMI GATC 6 cut(s) 142, 186, 499, 523, 1126, 1382
BssNI GRCGYC 1 cut(s) 93
BssT1I CCWWGG 3 cut(s) 329, 387, 439
Bst2UI CCWGG 1 cut(s) 537
Bst4CI ACNGT 4 cut(s) 163, 353, 415, 574
Bst6I CTCTTC 2 cut(s) 315, 777
BstACI GRCGYC 1 cut(s) 93
BstAFI CTTAAG 1 cut(s) 1346
BstAPI GCANNNNNTGC 1 cut(s) 776
BstC8I GCNNGC 6 cut(s) 76, 104, 136, 170, 193, 663
BstDEI CTNAG 6 cut(s) 1007, 1074, 1171, 1295, 1392, 1536
BstENI CCTNNNNNAGG 1 cut(s) 333
BstF5I GGATG 5 cut(s) 435, 609, 882, 1126, 1407
BstFNI CGCG 3 cut(s) 78, 132, 172
BstH2I RGCGCY 2 cut(s) 75, 1302
BstHHI GCGC 3 cut(s) 74, 134, 1301
BstKTI GATC 6 cut(s) 145, 189, 502, 526, 1129, 1385
BstMAI GTCTC 5 cut(s) 52, 84, 107, 1221, 1364
BstMBI GATC 6 cut(s) 142, 186, 499, 523, 1126, 1382
BstMCI CGRYCG 1 cut(s) 634
BstMWI GCNNNNNNNGC 6 cut(s) 23, 165, 767, 776, 1181, 1509
BstNI CCWGG 1 cut(s) 537
BstNSI RCATGY 2 cut(s) 665, 1414
BstPAI GACNNNNGTC 1 cut(s) 489
BstSCI CCNGG 6 cut(s) 85, 461, 535, 807, 808, 1313
BstSFI CTRYAG 2 cut(s) 573, 765
BstUI CGCG 3 cut(s) 78, 132, 172
BstV1I GCAGC 2 cut(s) 677, 1330
BstV2I GAAGAC 1 cut(s) 725
BsuI GTATCC 2 cut(s) 197, 828
BsuRI GGCC 6 cut(s) 14, 179, 1090, 1160, 1463, 1503
BtsCI GGATG 5 cut(s) 435, 609, 882, 1126, 1407
BtsIMutI CAGTG 2 cut(s) 396, 1537
BveI ACCTGC 1 cut(s) 1318
Cac8I GCNNGC 6 cut(s) 76, 104, 136, 170, 193, 663
CfoI GCGC 3 cut(s) 74, 134, 1301
Cfr10I RCCGGY 2 cut(s) 134, 191
Cfr13I GGNCC 6 cut(s) 177, 491, 533, 1159, 1283, 1416
Cfr9I CCCGGG 1 cut(s) 808
CpoI CGGWCCG 1 cut(s) 491
Csp6I GTAC 4 cut(s) 542, 1038, 1150, 1304
CspI CGGWCCG 1 cut(s) 491
CviAII CATG 8 cut(s) 239, 359, 527, 662, 980, 1027, 1069, 1411
CviQI GTAC 4 cut(s) 542, 1038, 1150, 1304
DdeI CTNAG 6 cut(s) 1007, 1074, 1171, 1295, 1392, 1536
DpnI GATC 6 cut(s) 144, 188, 501, 525, 1128, 1384
DpnII GATC 6 cut(s) 142, 186, 499, 523, 1126, 1382
DraIII CACNNNGTG 1 cut(s) 174
DrdI GACNNNNNNGTC 1 cut(s) 161
DseDI GACNNNNNNGTC 1 cut(s) 161
EaeI YGGCCR 3 cut(s) 12, 1461, 1501
Eam1104I CTCTTC 2 cut(s) 315, 777
EarI CTCTTC 2 cut(s) 315, 777
EciI GGCGGA 1 cut(s) 242
Ecl136II GAGCTC 1 cut(s) 1512
Eco130I CCWWGG 3 cut(s) 329, 387, 439
Eco24I GRGCYC 1 cut(s) 1514
Eco47I GGWCC 4 cut(s) 491, 533, 1283, 1416
Eco47III AGCGCT 1 cut(s) 1300
Eco53kI GAGCTC 1 cut(s) 1512
Eco57I CTGAAG 4 cut(s) 762, 801, 1185, 1455
Eco88I CYCGRG 2 cut(s) 545, 808
EcoICRI GAGCTC 1 cut(s) 1512
EcoNI CCTNNNNNAGG 1 cut(s) 333
EcoO109I RGGNCCY 1 cut(s) 1159
EcoRI GAATTC 1 cut(s) 588
EcoRII CCWGG 1 cut(s) 535
EcoT14I CCWWGG 3 cut(s) 329, 387, 439
EcoT22I ATGCAT 1 cut(s) 1230
EcoT38I GRGCYC 1 cut(s) 1514
ErhI CCWWGG 3 cut(s) 329, 387, 439
Esp3I CGTCTC 1 cut(s) 84
FaeI CATG 8 cut(s) 242, 362, 530, 665, 983, 1030, 1072, 1414
FalI AAGNNNNNCTT 2 cut(s) 1188, 1220
FaqI GGGAC 5 cut(s) 368, 685, 811, 1352, 1402
FatI CATG 8 cut(s) 238, 358, 526, 661, 979, 1026, 1068, 1410
FauI CCCGC 1 cut(s) 83
FauNDI CATATG 1 cut(s) 676
FbaI TGATCA 1 cut(s) 499
Fnu4HI GCNGC 4 cut(s) 15, 432, 666, 1319
FokI GGATG 5 cut(s) 422, 616, 889, 1133, 1414
FriOI GRGCYC 1 cut(s) 1514
Fsp4HI GCNGC 4 cut(s) 15, 432, 666, 1319
FspBI CTAG 3 cut(s) 330, 822, 1421
GlaI GCGC 3 cut(s) 73, 133, 1300
GluI GCNGC 4 cut(s) 15, 432, 666, 1319
GsuI CTGGAG 1 cut(s) 558
HaeII RGCGCY 2 cut(s) 75, 1302
HaeIII GGCC 6 cut(s) 14, 179, 1090, 1160, 1463, 1503
HapII CCGG 7 cut(s) 87, 135, 192, 462, 714, 809, 1314
HhaI GCGC 3 cut(s) 74, 134, 1301
Hin1I GRCGYC 1 cut(s) 93
Hin1II CATG 8 cut(s) 242, 362, 530, 665, 983, 1030, 1072, 1414
Hin6I GCGC 3 cut(s) 72, 132, 1299
HinP1I GCGC 3 cut(s) 72, 132, 1299
HincII GTYRAC 1 cut(s) 1111
HindII GTYRAC 1 cut(s) 1111
HindIII AAGCTT 2 cut(s) 682, 702
HinfI GANTC 7 cut(s) 4, 123, 339, 381, 710, 1272, 1527
HpaII CCGG 7 cut(s) 87, 135, 192, 462, 714, 809, 1314
HphI GGTGA 4 cut(s) 374, 866, 1007, 1369
Hpy166II GTNNAC 3 cut(s) 484, 1111, 1259
Hpy188I TCNGA 5 cut(s) 112, 495, 523, 910, 1077
Hpy188III TCNNGA 8 cut(s) 120, 593, 698, 714, 740, 1084, 1205, 1286
Hpy8I GTNNAC 3 cut(s) 484, 1111, 1259
Hpy99I CGWCG 7 cut(s) 23, 26, 32, 112, 143, 158, 167
HpyAV CCTTC 5 cut(s) 589, 737, 1075, 1160, 1506
HpyCH4III ACNGT 4 cut(s) 163, 353, 415, 574
HpyCH4IV ACGT 1 cut(s) 93
HpyCH4V TGCA 9 cut(s) 665, 761, 919, 953, 988, 1049, 1228, 1434, 1519
HpyF10VI GCNNNNNNNGC 6 cut(s) 23, 165, 767, 776, 1181, 1509
HpyF3I CTNAG 6 cut(s) 1007, 1074, 1171, 1295, 1392, 1536
HpySE526I ACGT 1 cut(s) 93
Hsp92I GRCGYC 1 cut(s) 93
Hsp92II CATG 8 cut(s) 242, 362, 530, 665, 983, 1030, 1072, 1414
HspAI GCGC 3 cut(s) 72, 132, 1299
Kpn2I TCCGGA 1 cut(s) 713
KroI GCCGGC 2 cut(s) 134, 191
KroNI GCCGGC 2 cut(s) 136, 193
Ksp22I TGATCA 1 cut(s) 499
Kzo9I GATC 6 cut(s) 142, 186, 499, 523, 1126, 1382
LmnI GCTCC 3 cut(s) 200, 1453, 1509
Lsp1109I GCAGC 2 cut(s) 677, 1330
LweI GCATC 4 cut(s) 229, 594, 779, 1111
MaeI CTAG 3 cut(s) 330, 822, 1421
MaeII ACGT 1 cut(s) 93
MaeIII GTNAC 5 cut(s) 409, 419, 568, 854, 887
MalI GATC 6 cut(s) 144, 188, 501, 525, 1128, 1384
MboI GATC 6 cut(s) 142, 186, 499, 523, 1126, 1382
MhlI GDGCHC 1 cut(s) 1514
MluCI AATT 4 cut(s) 211, 588, 961, 1199
MlyI GAGTC 2 cut(s) 348, 390
MmeI TCCRAC 2 cut(s) 90, 1487
Mph1103I ATGCAT 1 cut(s) 1230
MreI CGCCGGCG 1 cut(s) 134
MroI TCCGGA 1 cut(s) 713
MroNI GCCGGC 2 cut(s) 134, 191
MseI TTAA 1 cut(s) 1347
MslI CAYNNNNRTG 4 cut(s) 363, 1031, 1067, 1233
MspA1I CMGCKG 1 cut(s) 489
MspCI CTTAAG 1 cut(s) 1346
MspI CCGG 7 cut(s) 87, 135, 192, 462, 714, 809, 1314
MspR9I CCNGG 6 cut(s) 87, 463, 537, 809, 810, 1315
Mva1269I GAATGC 1 cut(s) 1230
MvaI CCWGG 1 cut(s) 537
MvnI CGCG 3 cut(s) 78, 132, 172
MwoI GCNNNNNNNGC 6 cut(s) 23, 165, 767, 776, 1181, 1509
NaeI GCCGGC 2 cut(s) 136, 193
NciI CCSGG 5 cut(s) 87, 463, 809, 810, 1315
NdeI CATATG 1 cut(s) 676
NdeII GATC 6 cut(s) 142, 186, 499, 523, 1126, 1382
NgoMIV GCCGGC 2 cut(s) 134, 191
NlaIII CATG 8 cut(s) 242, 362, 530, 665, 983, 1030, 1072, 1414
NlaIV GGNNCC 4 cut(s) 160, 196, 1161, 1418
NmeAIII GCCGAG 1 cut(s) 1489
NmuCI GTSAC 3 cut(s) 568, 854, 887
NsiI ATGCAT 1 cut(s) 1230
NspI RCATGY 2 cut(s) 665, 1414
PaeI GCATGC 1 cut(s) 665
PcsI WCGNNNNNNNCGW 3 cut(s) 27, 116, 159
PctI GAATGC 1 cut(s) 1230
PdiI GCCGGC 2 cut(s) 136, 193
PfeI GAWTC 4 cut(s) 123, 710, 1272, 1527
PfoI TCCNGGA 1 cut(s) 535
PkrI GCNGC 4 cut(s) 16, 433, 667, 1320
PleI GAGTC 2 cut(s) 347, 389
PpsI GAGTC 2 cut(s) 347, 389
PshAI GACNNNNGTC 1 cut(s) 489
PsiI TTATAA 1 cut(s) 375
Psp124BI GAGCTC 1 cut(s) 1514
Psp6I CCWGG 1 cut(s) 535
PspGI CCWGG 1 cut(s) 535
PspN4I GGNNCC 4 cut(s) 160, 196, 1161, 1418
PspPI GGNCC 6 cut(s) 177, 491, 533, 1159, 1283, 1416
RsaI GTAC 4 cut(s) 543, 1039, 1151, 1305
RsaNI GTAC 4 cut(s) 542, 1038, 1150, 1304
RseI CAYNNNNRTG 4 cut(s) 363, 1031, 1067, 1233
Rsr2I CGGWCCG 1 cut(s) 491
RsrII CGGWCCG 1 cut(s) 491
SacI GAGCTC 1 cut(s) 1514
SaqAI TTAA 1 cut(s) 1347
SatI GCNGC 4 cut(s) 15, 432, 666, 1319
Sau3AI GATC 6 cut(s) 142, 186, 499, 523, 1126, 1382
Sau96I GGNCC 6 cut(s) 177, 491, 533, 1159, 1283, 1416
ScaI AGTACT 1 cut(s) 543
SchI GAGTC 2 cut(s) 348, 390
ScrFI CCNGG 6 cut(s) 87, 463, 537, 809, 810, 1315
SduI GDGCHC 1 cut(s) 1514
SfaNI GCATC 4 cut(s) 229, 594, 779, 1111
SfcI CTRYAG 2 cut(s) 573, 765
SgrAI CRCCGGYG 1 cut(s) 134
SinI GGWCC 4 cut(s) 491, 533, 1283, 1416
SmaI CCCGGG 1 cut(s) 810
SmiMI CAYNNNNRTG 4 cut(s) 363, 1031, 1067, 1233
SmlI CTYRAG 4 cut(s) 901, 1084, 1185, 1346
SmoI CTYRAG 4 cut(s) 901, 1084, 1185, 1346
SphI GCATGC 1 cut(s) 665
Sse9I AATT 4 cut(s) 211, 588, 961, 1199
SspI AATATT 1 cut(s) 562
SspMI CTAG 3 cut(s) 330, 822, 1421
SstI GAGCTC 1 cut(s) 1514
StyD4I CCNGG 6 cut(s) 85, 461, 535, 807, 808, 1313
StyI CCWWGG 3 cut(s) 329, 387, 439
TaaI ACNGT 4 cut(s) 163, 353, 415, 574
TaiI ACGT 1 cut(s) 96
TaqI TCGA 2 cut(s) 119, 592
TasI AATT 4 cut(s) 211, 588, 961, 1199
TatI WGTACW 1 cut(s) 541
TauI GCSGC 2 cut(s) 17, 434
TfiI GAWTC 4 cut(s) 123, 710, 1272, 1527
Tru1I TTAA 1 cut(s) 1347
Tru9I TTAA 1 cut(s) 1347
TscAI CASTG 2 cut(s) 403, 1537
TseFI GTSAC 3 cut(s) 568, 854, 887
TseI GCWGC 2 cut(s) 665, 1318
Tsp45I GTSAC 3 cut(s) 568, 854, 887
TspDTI ATGAA 9 cut(s) 333, 499, 693, 855, 945, 1015, 1057, 1251, 1418
TspMI CCCGGG 1 cut(s) 808
TspRI CASTG 2 cut(s) 403, 1537
Vha464I CTTAAG 1 cut(s) 1346
VpaK11BI GGWCC 4 cut(s) 491, 533, 1283, 1416
XagI CCTNNNNNAGG 1 cut(s) 333
XapI RAATTY 2 cut(s) 588, 1199
XceI RCATGY 2 cut(s) 665, 1414
XmaI CCCGGG 1 cut(s) 808
XmaJI CCTAGG 1 cut(s) 329
XspI CTAG 3 cut(s) 330, 822, 1421
ZraI GACGTC 1 cut(s) 94
ZrmI AGTACT 1 cut(s) 543
Zsp2I ATGCAT 1 cut(s) 1230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.