Prupe.1G415000_v2.0.a1

Vacuolar-processing enzyme-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0009785
Physical Location & Seq
Forward (+)
36092586 .. 36096869
4284 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G415000.2

Sequence Viewer

Length: 1485 bp
ATGACTCGGTTGGCTAGCGCCGTCGTCCTGTTGTTCCTTGTCTCCCTCTCCAGTTTCGCTGCCGGTAGCCGGGACCTGATCGGAGACGTTTTACGGTTACCTTCTGAAGCTTCTAGATTCTTCGGCAGAGGCGATGACGGCCCTGATGAGCAAGACGACGGCACCGTGGGGACCAGATGGGCTGTTTTGATCGCTGGCTCAAATGGATACTGGAATTACAGGCATCAGGCTGATATTTGCCATGCTTATCAACTACTGAAGAAAGGTGGGCTGAAAGATGAAAACATTGTTGTATTCATGTATGATGATATCGCTTATAACGAAGAGAACCCTAGGCCCGGAGTCATCATTAACAGCCCACATGGTGATGACGTTTACAAAGGAGTCCCAAAGGATTACACCGGCGATGATGTTACTGTTAACAATTTTTTTGCTGCTATCCTTGGAAATAAAACTGCTCTTACAGGGGGTACTGGGAAGGTTGTGGACAGCGGTCCAAACGATCATATTTTCATTTACTATTCTGATCATGGTGGTCCTGGAGTTCTCGGGATGCCTACCAGTCCATATATTTATGCTAACGATCTAATAGAAGTCCTGAAGAAGAAGCACGCAGCAGGAACCTATAAAAGCTTGGTATTTTATCTTGAAGCTTGTGAATCCGGAAGTATCTTTGAGGGACTTCTTCCTGAAGGTTTGAATATCTTTGCAACTACGGCATCAAATGCAGAAGAGAGCAGTTGGGGGACTTACTGTCCAGGAGAGTATCCTAGTCCTCCCCCGGAGTATGAAACCTGCTTGGGTGACCTGTATAGCGTTGCTTGGATGGAAGACAGTGACATACACAACTTGAGGTCGGAAACTTTGCACCAACAGTATGAGCTGGTTAAAACGAGGACAGCAAATGACAATTCTGGCTTTGGTTCTCATGTCATGCAATATGGCGATGTAGGGCTCAGCAAGGAAAACCTCTTTGTGTATATGGGGACAAATCCTGCAAATGATAACTACACTTTTCTGGGCGAGAACTCCTTGAGGCCATCTACAAAAGCTGTCAACCAGCGGGATGCTGATCTTGTCCATTTCTGGCATAAGTACCGCAAAGCTCCTGAAGGCTCACCAAGGAAAATTCAAGCTCAAAAGGATTTTGTTGAAGCAATGTCTCATAGAATGCATATGGATCAAACCATGAAACTTATCGGGAAACTCCTGTTTGGAATCAAGAAAGGTCCAGAAGTGCTCAACACTGTTCGACCTGCTGGGCAGCCACTTGTTGATGACTGGGACTGCCTTAAGACAATGGTGAGGAGTTTTGAGACATATTGTGGATCCCTATCTCAATACGGGATGAAACACATGCGGTCCCTGGCAAACATCTGCAACGCCGGAATGACAAAGGAGCAGATGACCGAGGCTTCAGCACAAGCCTGCACGAGCGTTCCTTCTAGCCGTTGGAGCTCCCTGCACCGGGGGTTCAGCGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04612 map04142 map04612
Pfam Domains
Protein Families
Peptidase_C13

Protein Analysis

495

Amino Acids

54.53

Weight (kDa)

5.54

Isoelectric Point (pI)

43.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009785)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G25940 AT4G32940
fragaria_vesca FvH4_2g32190 FvH4_2g32190 FvH4_2g32190 FvH4_2g32190
malus_domestica MD08G1073500.v1.1
prunus_persica Prupe.1G415000_v2.0.a1 Prupe.1G415000_v2.0.a1
pyrus_communis pycom08g06090 pycom15g05790
rosa_chinensis RchiOBHm_Chr6g0310481
rosa_laevigata RLG00000010479
rosa_multiflora Rmu_sc0035020.1_g000006
rosa_roxburghii Rroxscaffold_7G00158490
rosa_rugosa Rorug06G0380700
rosa_samantha Rh6AG494700 Rh6BG504400 Rh6CG509600 Rh6DG495600
rosa_wichuraiana Rw6G043030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 318
Acc36I ACCTGC 2 cut(s) 803, 1264
AccB1I GGYRCC 1 cut(s) 161
AccIII TCCGGA 1 cut(s) 662
AciI CCGC 4 cut(s) 492, 1063, 1099, 1360
AclWI GGATC 3 cut(s) 1188, 1323, 1336
AcsI RAATTY 1 cut(s) 1128
AcuI CTGAAG 6 cut(s) 126, 278, 620, 711, 1131, 1401
AdeI CACNNNGTG 1 cut(s) 365
AfaI GTAC 2 cut(s) 472, 1097
AfiI CCNNNNNNNGG 4 cut(s) 69, 338, 1467, 1468
AflII CTTAAG 1 cut(s) 1292
AgsI TTSAA 4 cut(s) 650, 700, 1133, 1154
AhdI GACNNNNNGTC 1 cut(s) 753
AjnI CCWGG 3 cut(s) 538, 757, 1365
AluBI AGCT 8 cut(s) 110, 633, 653, 883, 1052, 1106, 1136, 1458
AluI AGCT 8 cut(s) 110, 633, 653, 883, 1052, 1106, 1136, 1458
Alw21I GWGCWC 2 cut(s) 1242, 1460
Alw26I GTCTC 4 cut(s) 46, 78, 1167, 1310
AlwI GGATC 3 cut(s) 1188, 1323, 1336
Ama87I CYCGRG 1 cut(s) 548
Aor13HI TCCGGA 1 cut(s) 662
AoxI GGCC 3 cut(s) 139, 335, 1037
ApeKI GCWGC 4 cut(s) 59, 434, 614, 1264
ApoI RAATTY 1 cut(s) 1128
AspA2I CCTAGG 1 cut(s) 332
AspLEI GCGC 2 cut(s) 20, 1481
AspS9I GGNCC 8 cut(s) 73, 140, 171, 336, 494, 536, 1229, 1362
AsuC2I CCSGG 4 cut(s) 71, 339, 782, 1469
AsuHPI GGTGA 4 cut(s) 377, 815, 1110, 1315
AsuNHI GCTAGC 1 cut(s) 14
AvaI CYCGRG 1 cut(s) 548
AvaII GGWCC 6 cut(s) 73, 171, 494, 536, 1229, 1362
AvrII CCTAGG 1 cut(s) 332
BamHI GGATCC 1 cut(s) 1328
BanI GGYRCC 1 cut(s) 161
BanII GRGCYC 2 cut(s) 957, 1460
BauI CACGAG 1 cut(s) 1432
BbsI GAAGAC 1 cut(s) 837
Bbv12I GWGCWC 2 cut(s) 1242, 1460
BbvI GCAGC 4 cut(s) 46, 421, 626, 1276
BccI CCATC 3 cut(s) 171, 820, 1048
BceAI ACGGC 5 cut(s) 5, 154, 175, 732, 1434
BciT130I CCWGG 3 cut(s) 540, 759, 1367
BciVI GTATCC 2 cut(s) 200, 777
BclI TGATCA 1 cut(s) 526
BcnI CCSGG 4 cut(s) 71, 339, 782, 1469
BcoDI GTCTC 4 cut(s) 46, 78, 1167, 1310
BfaI CTAG 5 cut(s) 15, 114, 333, 771, 1446
BfoI RGCGCY 1 cut(s) 21
BfrI CTTAAG 1 cut(s) 1292
BfuAI ACCTGC 2 cut(s) 803, 1264
BfuI GTATCC 2 cut(s) 200, 777
BisI GCNGC 4 cut(s) 60, 435, 615, 1265
BlnI CCTAGG 1 cut(s) 332
BlpI GCTNAGC 1 cut(s) 956
BlsI GCNGC 4 cut(s) 61, 436, 616, 1266
Bme1390I CCNGG 7 cut(s) 71, 339, 540, 759, 782, 1367, 1469
Bme18I GGWCC 6 cut(s) 73, 171, 494, 536, 1229, 1362
BmeRI GACNNNNNGTC 1 cut(s) 753
BmeT110I CYCGRG 1 cut(s) 548
BmgT120I GGNCC 8 cut(s) 73, 140, 171, 336, 494, 536, 1229, 1362
BmiI GGNNCC 6 cut(s) 74, 163, 172, 622, 1330, 1364
BmrFI CCNGG 7 cut(s) 71, 339, 540, 759, 782, 1367, 1469
BmrI ACTGGG 2 cut(s) 483, 1291
BmsI GCATC 4 cut(s) 232, 543, 728, 1057
BmtI GCTAGC 1 cut(s) 18
BmuI ACTGGG 2 cut(s) 483, 1291
BoxI GACNNNNGTC 1 cut(s) 492
BpiI GAAGAC 1 cut(s) 837
BpmI CTGGAG 2 cut(s) 34, 561
Bpu1102I GCTNAGC 1 cut(s) 956
BpuEI CTTGAG 2 cut(s) 871, 1054
BpuMI CCSGG 4 cut(s) 71, 339, 782, 1469
BsaBI GATNNNNATC 2 cut(s) 1071, 1333
BsaJI CCNNGG 8 cut(s) 165, 332, 442, 780, 1121, 1365, 1410, 1468
BsaWI WCCGGW 1 cut(s) 662
Bsc4I CCNNNNNNNGG 4 cut(s) 69, 338, 1467, 1468
Bse118I RCCGGY 2 cut(s) 62, 401
Bse1I ACTGG 5 cut(s) 51, 215, 478, 561, 1286
Bse3DI GCAATG 1 cut(s) 1164
Bse8I GATNNNNATC 2 cut(s) 1071, 1333
BseAI TCCGGA 1 cut(s) 662
BseBI CCWGG 3 cut(s) 540, 759, 1367
BseDI CCNNGG 8 cut(s) 165, 332, 442, 780, 1121, 1365, 1410, 1468
BseGI GGATG 4 cut(s) 558, 831, 1072, 1353
BseJI GATNNNNATC 2 cut(s) 1071, 1333
BseLI CCNNNNNNNGG 4 cut(s) 69, 338, 1467, 1468
BseMI GCAATG 1 cut(s) 1164
BseMII CTCAG 1 cut(s) 970
BseNI ACTGG 5 cut(s) 51, 215, 478, 561, 1286
BseRI GAGGAG 1 cut(s) 1321
BseXI GCAGC 4 cut(s) 46, 421, 626, 1276
BseYI CCCAGC 1 cut(s) 1259
BsgI GTGCAG 2 cut(s) 1414, 1448
BshFI GGCC 3 cut(s) 141, 337, 1039
BshNI GGYRCC 1 cut(s) 161
BsiHKAI GWGCWC 2 cut(s) 1242, 1460
BsiHKCI CYCGRG 1 cut(s) 548
BsiSI CCGG 8 cut(s) 63, 70, 339, 402, 663, 782, 1386, 1468
BslFI GGGAC 8 cut(s) 86, 184, 371, 693, 760, 1000, 1298, 1348
BslI CCNNNNNNNGG 4 cut(s) 69, 338, 1467, 1468
BsmAI GTCTC 4 cut(s) 46, 78, 1167, 1310
BsmBI CGTCTC 1 cut(s) 78
BsmFI GGGAC 8 cut(s) 86, 184, 371, 693, 760, 1000, 1298, 1348
BsmI GAATGC 1 cut(s) 1176
BsnI GGCC 3 cut(s) 141, 337, 1039
BsoBI CYCGRG 1 cut(s) 548
Bsp1286I GDGCHC 3 cut(s) 957, 1242, 1460
Bsp13I TCCGGA 1 cut(s) 662
Bsp143I GATC 8 cut(s) 78, 189, 502, 526, 583, 1072, 1180, 1328
Bsp1720I GCTNAGC 1 cut(s) 956
BspACI CCGC 4 cut(s) 492, 1063, 1099, 1360
BspANI GGCC 3 cut(s) 141, 337, 1039
BspCNI CTCAG 1 cut(s) 969
BspEI TCCGGA 1 cut(s) 662
BspLI GGNNCC 6 cut(s) 74, 163, 172, 622, 1330, 1364
BspMI ACCTGC 2 cut(s) 803, 1264
BspOI GCTAGC 1 cut(s) 18
BspPI GGATC 3 cut(s) 1188, 1323, 1336
BspT107I GGYRCC 1 cut(s) 161
BspTI CTTAAG 1 cut(s) 1292
BsrDI GCAATG 1 cut(s) 1164
BsrFI RCCGGY 2 cut(s) 62, 401
BsrI ACTGG 5 cut(s) 51, 215, 478, 561, 1286
BssAI RCCGGY 2 cut(s) 62, 401
BssECI CCNNGG 8 cut(s) 165, 332, 442, 780, 1121, 1365, 1410, 1468
BssMI GATC 8 cut(s) 78, 189, 502, 526, 583, 1072, 1180, 1328
BssSI CACGAG 1 cut(s) 1432
BssT1I CCWWGG 3 cut(s) 332, 442, 1121
Bst2BI CACGAG 1 cut(s) 1432
Bst2UI CCWGG 3 cut(s) 540, 759, 1367
Bst4CI ACNGT 7 cut(s) 96, 166, 418, 755, 836, 876, 1249
Bst6I CTCTTC 2 cut(s) 318, 726
BstAFI CTTAAG 1 cut(s) 1292
BstAPI GCANNNNNTGC 1 cut(s) 725
BstC8I GCNNGC 4 cut(s) 16, 196, 612, 1429
BstDEI CTNAG 1 cut(s) 956
BstDSI CCRYGG 1 cut(s) 165
BstEII GGTNACC 2 cut(s) 96, 803
BstF5I GGATG 4 cut(s) 558, 831, 1072, 1353
BstH2I RGCGCY 1 cut(s) 21
BstHHI GCGC 2 cut(s) 20, 1481
BstKTI GATC 8 cut(s) 81, 192, 505, 529, 586, 1075, 1183, 1331
BstMAI GTCTC 4 cut(s) 46, 78, 1167, 1310
BstMBI GATC 8 cut(s) 78, 189, 502, 526, 583, 1072, 1180, 1328
BstMWI GCNNNNNNNGC 4 cut(s) 138, 716, 725, 1455
BstNI CCWGG 3 cut(s) 540, 759, 1367
BstNSI RCATGY 1 cut(s) 1360
BstPAI GACNNNNGTC 1 cut(s) 492
BstPI GGTNACC 2 cut(s) 96, 803
BstSCI CCNGG 7 cut(s) 69, 337, 538, 757, 780, 1365, 1467
BstV1I GCAGC 4 cut(s) 46, 421, 626, 1276
BstV2I GAAGAC 1 cut(s) 837
BstX2I RGATCY 1 cut(s) 1328
BstYI RGATCY 1 cut(s) 1328
BsuI GTATCC 2 cut(s) 200, 777
BsuRI GGCC 3 cut(s) 141, 337, 1039
BtgI CCRYGG 1 cut(s) 165
BtgZI GCGATG 3 cut(s) 147, 420, 960
BtsCI GGATG 4 cut(s) 558, 831, 1072, 1353
BtsIMutI CAGTG 2 cut(s) 841, 1245
BveI ACCTGC 2 cut(s) 803, 1264
Cac8I GCNNGC 4 cut(s) 16, 196, 612, 1429
CfoI GCGC 2 cut(s) 20, 1481
Cfr10I RCCGGY 2 cut(s) 62, 401
Cfr13I GGNCC 8 cut(s) 73, 140, 171, 336, 494, 536, 1229, 1362
Csp6I GTAC 2 cut(s) 471, 1096
CviAII CATG 9 cut(s) 242, 298, 362, 530, 929, 934, 1189, 1357, 1482
CviQI GTAC 2 cut(s) 471, 1096
DdeI CTNAG 1 cut(s) 956
DpnI GATC 8 cut(s) 80, 191, 504, 528, 585, 1074, 1182, 1330
DpnII GATC 8 cut(s) 78, 189, 502, 526, 583, 1072, 1180, 1328
DraIII CACNNNGTG 1 cut(s) 365
DriI GACNNNNNGTC 1 cut(s) 753
Eam1104I CTCTTC 2 cut(s) 318, 726
Eam1105I GACNNNNNGTC 1 cut(s) 753
EarI CTCTTC 2 cut(s) 318, 726
Ecl136II GAGCTC 1 cut(s) 1458
Eco130I CCWWGG 3 cut(s) 332, 442, 1121
Eco24I GRGCYC 2 cut(s) 957, 1460
Eco32I GATATC 1 cut(s) 310
Eco47I GGWCC 6 cut(s) 73, 171, 494, 536, 1229, 1362
Eco53kI GAGCTC 1 cut(s) 1458
Eco57I CTGAAG 6 cut(s) 126, 278, 620, 711, 1131, 1401
Eco88I CYCGRG 1 cut(s) 548
Eco91I GGTNACC 2 cut(s) 96, 803
EcoICRI GAGCTC 1 cut(s) 1458
EcoO109I RGGNCCY 1 cut(s) 73
EcoO65I GGTNACC 2 cut(s) 96, 803
EcoRII CCWGG 3 cut(s) 538, 757, 1365
EcoRV GATATC 1 cut(s) 310
EcoT14I CCWWGG 3 cut(s) 332, 442, 1121
EcoT22I ATGCAT 1 cut(s) 1176
EcoT38I GRGCYC 2 cut(s) 957, 1460
ErhI CCWWGG 3 cut(s) 332, 442, 1121
Esp3I CGTCTC 1 cut(s) 78
FaeI CATG 9 cut(s) 245, 301, 365, 533, 932, 937, 1192, 1360, 1485
FaqI GGGAC 8 cut(s) 86, 184, 371, 693, 760, 1000, 1298, 1348
FatI CATG 9 cut(s) 241, 297, 361, 529, 928, 933, 1188, 1356, 1481
FauI CCCGC 1 cut(s) 1056
FauNDI CATATG 1 cut(s) 1176
FbaI TGATCA 1 cut(s) 526
Fnu4HI GCNGC 4 cut(s) 60, 435, 615, 1265
FokI GGATG 4 cut(s) 565, 838, 1079, 1360
FriOI GRGCYC 2 cut(s) 957, 1460
Fsp4HI GCNGC 4 cut(s) 60, 435, 615, 1265
FspBI CTAG 5 cut(s) 15, 114, 333, 771, 1446
GlaI GCGC 2 cut(s) 19, 1480
GluI GCNGC 4 cut(s) 60, 435, 615, 1265
GsaI CCCAGC 1 cut(s) 1263
GsuI CTGGAG 2 cut(s) 34, 561
HaeII RGCGCY 1 cut(s) 21
HaeIII GGCC 3 cut(s) 141, 337, 1039
HapII CCGG 8 cut(s) 63, 70, 339, 402, 663, 782, 1386, 1468
HhaI GCGC 2 cut(s) 20, 1481
Hin1II CATG 9 cut(s) 245, 301, 365, 533, 932, 937, 1192, 1360, 1485
Hin6I GCGC 2 cut(s) 18, 1479
HinP1I GCGC 2 cut(s) 18, 1479
HincII GTYRAC 2 cut(s) 421, 1057
HindII GTYRAC 2 cut(s) 421, 1057
HindIII AAGCTT 3 cut(s) 108, 631, 651
HinfI GANTC 6 cut(s) 4, 117, 342, 384, 659, 1218
HpaI GTTAAC 1 cut(s) 421
HpaII CCGG 8 cut(s) 63, 70, 339, 402, 663, 782, 1386, 1468
HphI GGTGA 4 cut(s) 377, 815, 1110, 1315
Hpy166II GTNNAC 4 cut(s) 376, 421, 487, 1057
Hpy188I TCNGA 4 cut(s) 83, 106, 526, 859
Hpy8I GTNNAC 4 cut(s) 376, 421, 487, 1057
Hpy99I CGWCG 2 cut(s) 26, 161
HpyAV CCTTC 5 cut(s) 111, 472, 686, 1106, 1452
HpyCH4III ACNGT 7 cut(s) 96, 166, 418, 755, 836, 876, 1249
HpyCH4IV ACGT 2 cut(s) 87, 372
HpyCH4V TGCA 9 cut(s) 710, 728, 868, 937, 998, 1174, 1380, 1431, 1465
HpyF10VI GCNNNNNNNGC 4 cut(s) 138, 716, 725, 1455
HpyF3I CTNAG 1 cut(s) 956
HpySE526I ACGT 2 cut(s) 87, 372
Hsp92II CATG 9 cut(s) 245, 301, 365, 533, 932, 937, 1192, 1360, 1485
HspAI GCGC 2 cut(s) 18, 1479
Kpn2I TCCGGA 1 cut(s) 662
Ksp22I TGATCA 1 cut(s) 526
KspAI GTTAAC 1 cut(s) 421
Kzo9I GATC 8 cut(s) 78, 189, 502, 526, 583, 1072, 1180, 1328
LmnI GCTCC 4 cut(s) 1111, 1399, 1455, 1463
Lsp1109I GCAGC 4 cut(s) 46, 421, 626, 1276
LweI GCATC 4 cut(s) 232, 543, 728, 1057
MaeI CTAG 5 cut(s) 15, 114, 333, 771, 1446
MaeII ACGT 2 cut(s) 87, 372
MaeIII GTNAC 4 cut(s) 96, 412, 803, 836
MalI GATC 8 cut(s) 80, 191, 504, 528, 585, 1074, 1182, 1330
MboI GATC 8 cut(s) 78, 189, 502, 526, 583, 1072, 1180, 1328
MboII GAAGA 8 cut(s) 112, 271, 335, 613, 616, 677, 743, 842
MflI RGATCY 1 cut(s) 1328
MhlI GDGCHC 3 cut(s) 957, 1242, 1460
MluCI AATT 4 cut(s) 214, 424, 910, 1128
MlyI GAGTC 2 cut(s) 351, 393
MmeI TCCRAC 2 cut(s) 837, 1433
Mph1103I ATGCAT 1 cut(s) 1176
MroI TCCGGA 1 cut(s) 662
MseI TTAA 4 cut(s) 351, 420, 888, 1293
MslI CAYNNNNRTG 1 cut(s) 366
MspA1I CMGCKG 2 cut(s) 492, 1063
MspCI CTTAAG 1 cut(s) 1292
MspI CCGG 8 cut(s) 63, 70, 339, 402, 663, 782, 1386, 1468
MspR9I CCNGG 7 cut(s) 71, 339, 540, 759, 782, 1367, 1469
Mva1269I GAATGC 1 cut(s) 1176
MvaI CCWGG 3 cut(s) 540, 759, 1367
MwoI GCNNNNNNNGC 4 cut(s) 138, 716, 725, 1455
NciI CCSGG 4 cut(s) 71, 339, 782, 1469
NdeI CATATG 1 cut(s) 1176
NdeII GATC 8 cut(s) 78, 189, 502, 526, 583, 1072, 1180, 1328
NheI GCTAGC 1 cut(s) 14
NlaIII CATG 9 cut(s) 245, 301, 365, 533, 932, 937, 1192, 1360, 1485
NlaIV GGNNCC 6 cut(s) 74, 163, 172, 622, 1330, 1364
NmuCI GTSAC 2 cut(s) 803, 836
NsiI ATGCAT 1 cut(s) 1176
NspI RCATGY 1 cut(s) 1360
PcsI WCGNNNNNNNCGW 3 cut(s) 129, 162, 318
PctI GAATGC 1 cut(s) 1176
PfeI GAWTC 3 cut(s) 117, 659, 1218
PfoI TCCNGGA 2 cut(s) 538, 757
PkrI GCNGC 4 cut(s) 61, 436, 616, 1266
PleI GAGTC 2 cut(s) 350, 392
PpsI GAGTC 2 cut(s) 350, 392
PpuMI RGGWCCY 1 cut(s) 73
PshAI GACNNNNGTC 1 cut(s) 492
PsiI TTATAA 1 cut(s) 318
Psp124BI GAGCTC 1 cut(s) 1460
Psp5II RGGWCCY 1 cut(s) 73
Psp6I CCWGG 3 cut(s) 538, 757, 1365
PspEI GGTNACC 2 cut(s) 96, 803
PspFI CCCAGC 1 cut(s) 1259
PspGI CCWGG 3 cut(s) 538, 757, 1365
PspN4I GGNNCC 6 cut(s) 74, 163, 172, 622, 1330, 1364
PspPI GGNCC 8 cut(s) 73, 140, 171, 336, 494, 536, 1229, 1362
PspPPI RGGWCCY 1 cut(s) 73
PsuI RGATCY 1 cut(s) 1328
RsaI GTAC 2 cut(s) 472, 1097
RsaNI GTAC 2 cut(s) 471, 1096
RseI CAYNNNNRTG 1 cut(s) 366
SacI GAGCTC 1 cut(s) 1460
SaqAI TTAA 4 cut(s) 351, 420, 888, 1293
SatI GCNGC 4 cut(s) 60, 435, 615, 1265
Sau3AI GATC 8 cut(s) 78, 189, 502, 526, 583, 1072, 1180, 1328
Sau96I GGNCC 8 cut(s) 73, 140, 171, 336, 494, 536, 1229, 1362
SchI GAGTC 2 cut(s) 351, 393
ScrFI CCNGG 7 cut(s) 71, 339, 540, 759, 782, 1367, 1469
SduI GDGCHC 3 cut(s) 957, 1242, 1460
SfaNI GCATC 4 cut(s) 232, 543, 728, 1057
SgrAI CRCCGGYG 1 cut(s) 401
SinI GGWCC 6 cut(s) 73, 171, 494, 536, 1229, 1362
SmiMI CAYNNNNRTG 1 cut(s) 366
SmlI CTYRAG 3 cut(s) 850, 1033, 1292
SmoI CTYRAG 3 cut(s) 850, 1033, 1292
Sse9I AATT 4 cut(s) 214, 424, 910, 1128
SsiI CCGC 4 cut(s) 492, 1063, 1099, 1360
SspMI CTAG 5 cut(s) 15, 114, 333, 771, 1446
SstI GAGCTC 1 cut(s) 1460
StyD4I CCNGG 7 cut(s) 69, 337, 538, 757, 780, 1365, 1467
StyI CCWWGG 3 cut(s) 332, 442, 1121
TaaI ACNGT 7 cut(s) 96, 166, 418, 755, 836, 876, 1249
TaiI ACGT 2 cut(s) 90, 375
TaqI TCGA 1 cut(s) 1252
TaqII GACCGA 1 cut(s) 1424
TasI AATT 4 cut(s) 214, 424, 910, 1128
TfiI GAWTC 3 cut(s) 117, 659, 1218
Tru1I TTAA 4 cut(s) 351, 420, 888, 1293
Tru9I TTAA 4 cut(s) 351, 420, 888, 1293
TscAI CASTG 2 cut(s) 841, 1252
TseFI GTSAC 2 cut(s) 803, 836
TseI GCWGC 4 cut(s) 59, 434, 614, 1264
Tsp45I GTSAC 2 cut(s) 803, 836
TspDTI ATGAA 6 cut(s) 286, 294, 502, 804, 1205, 1364
TspRI CASTG 2 cut(s) 841, 1252
Vha464I CTTAAG 1 cut(s) 1292
VpaK11BI GGWCC 6 cut(s) 73, 171, 494, 536, 1229, 1362
XapI RAATTY 1 cut(s) 1128
XbaI TCTAGA 1 cut(s) 113
XceI RCATGY 1 cut(s) 1360
XmaJI CCTAGG 1 cut(s) 332
XspI CTAG 5 cut(s) 15, 114, 333, 771, 1446
Zsp2I ATGCAT 1 cut(s) 1176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.