Rroxscaffold_7G00158490

Vacuolar-processing enzyme-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0009785
Physical Location & Seq
Forward (+)
1910312 .. 1920858
10547 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00158490.1

Sequence Viewer

Length: 2541 bp
ATGACTCGGTTGGCCACCGTCGTCGCCGTCCTTCTTTTCTTCCTAGTCGCCCTCTCTTCTTCCGCCACCAGTAGCCGCGACCTGATCGGAGACGTCTTGCGCTTGCCGTCGGAAGCTTCGAGGTTCTTCCGCGCCGGCGATGGTGGCGCCGATCAGCAAGACGACGGCTCCGTCGGCACGCGGTGGGCCGTTTTGATCGCCGGCTCCAATGGATACTGGAATTACAGGCATCAGGCTGATATATGCCATGCCTATCAATTATTGAAGAAAGGCGGGCTGAAAGATGAAAATATTGTTGTATTTATGTATGATGATATTGCTTACAATAAAGAGAACCCAAGGCAAGGAGTCATAATCAACAGTCCTCACGGTGATGATGTTTATAAAGGAGTTCCGAAGGATTACACTGGGGATGATGTTACTGTTAATAATTTCTTTGCTGCTATTCTATTGAGTAGCAACTTAGATGAAACGATTTGTGTTGTTGTAGGGATGCCTACCAGTCCCTATATTTATGCAGACAGGCTCATAGAAGTTCTAAAGAAGAAGCATGCTGCAGGGACATATGAAAGTTTGGTATTTTATCTTGAAGCTTGTGAATCCGGAAGTATCTTTGAGGGTCTTCTTCCTGAAGGTTTGAATATCTTTGCAACTACAGCATCAAATGCAGAAGAGAGCAGTTGGGGGACTTACTGCCCGGGAGAGTATCCTAGTCCTCCCCCAGAATATGAAACCTGTTTGGGTGACTTGTATAGTGTTGCTTGGATGGAAGATAGTGACATGCACAACTTGAAGTCAGAAACTTTGCACCAGCAGTATGAACTGGTCAAATCAAGGACAGCGAGTGATAATTCTGCATATGGTTCTCATGTCATGCAATATGGTGACATAGGGCTCAGCAAGAACAACCTCTTCATGTATATGGGGACAAATCCTGCAAATGATAACTACACTTTCATGCCTCAGAACTTCTTCAGGCCATCTTCAAAAGCTGTCAACCAGAGGGATGCTGATCTGGTCCATTTCTGGGATAAGTTCCGCAAGGCTCCTGAAGGCTCACCAAGGAAAGCTCAAGCCCAAAAAGAATTTCTTGAAGCAATGTCTCACAGAATGCATATAGATGAAAGCATGAAACTTATCGGGAAACTCCTGTTTGGAATCAAAAAAGGTCCAGAGGTCCTCAGCGCTGTTCGACCTGCTGGGCAGCCACTTGTTGATGACTGGGACTGTCTCAAGACAATGGTGAGGAGTTTTGAGACATATTGCGGATCTCTATCTCAGTATGGGATGAAACACATGCGTTCCCTGGCAAACATCTGCAACGCTGGAATGACGAAGGAGCAGATGGCTGAGGCTTCAGCCCAAGCCTGCGTCAATGTTCCTTCTGGCCGATGGAGCTCTCTGCACAGGGGATTCAGATCTAATTCGAAGCTCCGTCTCTGTGTTCGTGTTCAGAGAGAAAACCAGAAGCTCGGAAAGATACATTCGGGGGGTTTCGGAACGTATTCCAAGTGGAATCGAGAGGTGATCGAGAATTTCAAGCTTAGAGGCACATTTTCAAGAGATTTGAGCGGTTTCGTGAAGAATCCTTCTGGTGCGCCAGCGGAGCAAGATTTCCGAAACGACGAAGTTTCTTCTGCGAGTTTAATCTTAGTAATACAGACAGGTGTACAAAAGGAGATGGAAAGAGAGTATGTAAGGCACGCCAATATTAGCGACACCAAGAATTCTAGAGACAGAACCGGAGGGGATAGCTCACTGCTGTTGAGATCGAGTCCTGATAAAACGACGACGTCCGAGTTCCTTTTGCAGTGGGGGAATCGCAAGCGCCTCCGGTGCATGAAGATCCAGGTCAAGGACGACTCCAGTGGTGTCCCGGTTACCAGAACCACGGTTCGGGTAGACCGCCGGGTCATTCGACCGGACAAGGACTCGTCCAATCAGCCGTCTACCACCAATAATAGTAACGGGTATTTGAACCTCCGTCAACGACCGTTATCGCCGCAACAACCACCACCTCAGCGTGTTCTCAGAAACTCGGAGACTTCAAGTGCAGGCATGAGAGGCCAGAGCAACGGAGCTATGAGGGGAATTATTTCGCCTGACAGGGGCGGCGTTGCGCACGATAAGAAAGGTACAACTAACAACCACAATCATCACCATCACCATAATGACAATCATCACAACCACAAGTCCGCTGCGTCATCGGAGACGGCGCACGATAGCAAGAAGGGCGGCTCCTCATCGGGGAGCGGCGACGCGGCAGCAGCACCACCGATGGTGTGGCCGCCCAAGTTCGTGATTGCCTTGACCAACAAAGAGAAGGAAGAGGATTTCATGGCCATTAAGGGATCCAAGCTCCCTCAGAGACCCAAAAAGAGAGCAAAGATCATCCAACGCACCCTTAATCTGGTAAGCCCGGGTGCATGGCTCTGTGATCTGACGCTGGAACGGTACGAGGTCAGAGAGAAGAAGATCTCCAAGAAGAGACCGAGAGGATTGAAGGCGATGGGCACCATGGTGGAGTCGGACTCGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04612 map04142 map04612
Pfam Domains
Protein Families
Peptidase_C13

Protein Analysis

846

Amino Acids

94.16

Weight (kDa)

9.03

Isoelectric Point (pI)

46.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C13 PF01650 61 - 310 5.8e-91 Peptidase C13 family
Legum_prodom PF20985 362 - 457 7.6e-43 Legumain, prodomain
DUF1639 PF07797 770 - 819 2.4e-22 Protein of unknown function (DUF1639)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009785)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G25940 AT4G32940
fragaria_vesca FvH4_2g32190 FvH4_2g32190 FvH4_2g32190 FvH4_2g32190
malus_domestica MD08G1073500.v1.1
prunus_persica Prupe.1G415000_v2.0.a1 Prupe.1G415000_v2.0.a1
pyrus_communis pycom08g06090 pycom15g05790
rosa_chinensis RchiOBHm_Chr6g0310481
rosa_laevigata RLG00000010479
rosa_multiflora Rmu_sc0035020.1_g000006
rosa_roxburghii Rroxscaffold_7G00158490
rosa_rugosa Rorug06G0380700
rosa_samantha Rh6AG494700 Rh6BG504400 Rh6CG509600 Rh6DG495600
rosa_wichuraiana Rw6G043030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 384
AasI GACNNNNNNGTC 2 cut(s) 170, 1910
AatII GACGTC 2 cut(s) 96, 1796
Acc16I TGCGCA 1 cut(s) 2121
Acc36I ACCTGC 1 cut(s) 1204
AccB1I GGYRCC 2 cut(s) 146, 2513
AccBSI CCGCTC 2 cut(s) 1572, 2253
AccI GTMKAC 2 cut(s) 1902, 1949
AccII CGCG 4 cut(s) 78, 132, 181, 2261
AccIII TCCGGA 1 cut(s) 602
AclWI GGATC 4 cut(s) 1276, 1840, 2346, 2359
AcoI YGGCCR 4 cut(s) 12, 1387, 2285, 2340
AcsI RAATTY 3 cut(s) 1085, 1534, 1726
AcuI CTGAAG 4 cut(s) 651, 958, 1071, 1341
AcyI GRCGYC 3 cut(s) 93, 147, 1793
AdeI CACNNNGTG 1 cut(s) 183
AfaI GTAC 3 cut(s) 1671, 2137, 2456
AfeI AGCGCT 1 cut(s) 1186
AfiI CCNNNNNNNGG 7 cut(s) 344, 1027, 1855, 1896, 2108, 2247, 2410
AjnI CCWGG 2 cut(s) 1305, 1848
AjuI GAANNNNNNNTTGG 2 cut(s) 1071, 1103
AleI CACNNNNGTG 1 cut(s) 2519
Alw21I GWGCWC 1 cut(s) 1400
AlwI GGATC 4 cut(s) 1276, 1840, 2346, 2359
Ama87I CYCGRG 2 cut(s) 697, 2419
Aor13HI TCCGGA 1 cut(s) 602
Aor51HI AGCGCT 1 cut(s) 1186
AoxI GGCC 7 cut(s) 12, 186, 977, 1387, 2065, 2285, 2340
ApeKI GCWGC 6 cut(s) 440, 554, 1204, 2198, 2264, 2267
ApoI RAATTY 3 cut(s) 1085, 1534, 1726
Asp700I GAANNNNTTC 3 cut(s) 971, 1504, 2095
AspLEI GCGC 8 cut(s) 102, 134, 149, 1187, 1600, 1830, 2122, 2218
AspS9I GGNCC 4 cut(s) 186, 1018, 1169, 1177
AsuC2I CCSGG 6 cut(s) 698, 699, 1877, 1910, 2420, 2421
AsuHPI GGTGA 8 cut(s) 383, 755, 896, 1050, 1255, 1537, 2150, 2156
AsuII TTCGAA 1 cut(s) 1427
AvaI CYCGRG 2 cut(s) 697, 2419
AvaII GGWCC 3 cut(s) 1018, 1169, 1177
BaeGI GKGCMC 1 cut(s) 2516
BalI TGGCCA 2 cut(s) 14, 2342
BamHI GGATCC 1 cut(s) 2351
BanI GGYRCC 2 cut(s) 146, 2513
BanII GRGCYC 2 cut(s) 897, 1400
BbsI GAAGAC 1 cut(s) 614
Bbv12I GWGCWC 1 cut(s) 1400
BbvCI CCTCAGC 3 cut(s) 1181, 1350, 2019
BbvI GCAGC 6 cut(s) 427, 541, 1216, 2185, 2276, 2279
BccI CCATC 9 cut(s) 134, 760, 988, 1339, 1386, 1675, 2169, 2272, 2503
BceAI ACGGC 6 cut(s) 11, 91, 173, 181, 1930, 2229
BciT130I CCWGG 2 cut(s) 1307, 1850
BciVI GTATCC 2 cut(s) 206, 717
BcnI CCSGG 6 cut(s) 698, 699, 1877, 1910, 2420, 2421
BfaI CTAG 3 cut(s) 44, 711, 1731
BfmI CTRYAG 2 cut(s) 555, 654
BfoI RGCGCY 3 cut(s) 150, 1188, 1831
BfuAI ACCTGC 1 cut(s) 1204
BfuI GTATCC 2 cut(s) 206, 717
BglII AGATCT 2 cut(s) 1418, 2475
BlpI GCTNAGC 1 cut(s) 896
Bme1390I CCNGG 8 cut(s) 698, 699, 1307, 1850, 1877, 1910, 2420, 2421
Bme18I GGWCC 3 cut(s) 1018, 1169, 1177
BmeT110I CYCGRG 2 cut(s) 697, 2419
BmgT120I GGNCC 4 cut(s) 186, 1018, 1169, 1177
BmiI GGNNCC 7 cut(s) 148, 169, 205, 1047, 2239, 2353, 2515
BmrFI CCNGG 8 cut(s) 698, 699, 1307, 1850, 1877, 1910, 2420, 2421
BmrI ACTGGG 2 cut(s) 417, 1231
BmsI GCATC 4 cut(s) 238, 483, 668, 997
BmuI ACTGGG 2 cut(s) 417, 1231
BpiI GAAGAC 1 cut(s) 614
BplI GAGNNNNNCTC 1 cut(s) 2516
BpmI CTGGAG 1 cut(s) 1849
Bpu10I CCTNAGC 3 cut(s) 1181, 1350, 2019
Bpu1102I GCTNAGC 1 cut(s) 896
Bpu14I TTCGAA 1 cut(s) 1427
BpuEI CTTGAG 2 cut(s) 1056, 1217
BpuMI CCSGG 6 cut(s) 698, 699, 1877, 1910, 2420, 2421
BsaBI GATNNNNATC 3 cut(s) 1011, 1273, 1417
BsaHI GRCGYC 3 cut(s) 93, 147, 1793
BsaI GGTCTC 2 cut(s) 2362, 2482
BsaJI CCNNGG 7 cut(s) 338, 697, 1061, 1305, 1890, 2419, 2517
BsaWI WCCGGW 4 cut(s) 602, 1742, 1833, 1921
BsaXI ACNNNNNCTCC 2 cut(s) 152, 182
Bsc4I CCNNNNNNNGG 7 cut(s) 344, 1027, 1855, 1896, 2108, 2247, 2410
Bse118I RCCGGY 2 cut(s) 134, 200
Bse1I ACTGG 7 cut(s) 69, 221, 412, 501, 828, 1226, 1866
Bse3DI GCAATG 1 cut(s) 1104
Bse8I GATNNNNATC 3 cut(s) 1011, 1273, 1417
BseAI TCCGGA 1 cut(s) 602
BseBI CCWGG 2 cut(s) 1307, 1850
BseDI CCNNGG 7 cut(s) 338, 697, 1061, 1305, 1890, 2419, 2517
BseGI GGATG 6 cut(s) 418, 498, 771, 1012, 1293, 2391
BseJI GATNNNNATC 3 cut(s) 1011, 1273, 1417
BseLI CCNNNNNNNGG 7 cut(s) 344, 1027, 1855, 1896, 2108, 2247, 2410
BseMI GCAATG 1 cut(s) 1104
BseMII CTCAG 8 cut(s) 910, 977, 1195, 1292, 1341, 2033, 2044, 2378
BseNI ACTGG 7 cut(s) 69, 221, 412, 501, 828, 1226, 1866
BseRI GAGGAG 2 cut(s) 1261, 2230
BseSI GKGCMC 1 cut(s) 2516
BseXI GCAGC 6 cut(s) 427, 541, 1216, 2185, 2276, 2279
BseYI CCCAGC 1 cut(s) 1199
BsgI GTGCAG 2 cut(s) 1388, 2073
Bsh1236I CGCG 4 cut(s) 78, 132, 181, 2261
Bsh1285I CGRYCG 2 cut(s) 1922, 1994
BshFI GGCC 7 cut(s) 14, 188, 979, 1389, 2067, 2287, 2342
BshNI GGYRCC 2 cut(s) 146, 2513
BsiEI CGRYCG 2 cut(s) 1922, 1994
BsiHKAI GWGCWC 1 cut(s) 1400
BsiHKCI CYCGRG 2 cut(s) 697, 2419
BslFI GGGAC 6 cut(s) 489, 574, 700, 940, 1238, 1859
BslI CCNNNNNNNGG 7 cut(s) 344, 1027, 1855, 1896, 2108, 2247, 2410
BsmBI CGTCTC 3 cut(s) 84, 1442, 2204
BsmFI GGGAC 6 cut(s) 489, 574, 700, 940, 1238, 1859
BsmI GAATGC 1 cut(s) 1116
BsnI GGCC 7 cut(s) 14, 188, 979, 1389, 2067, 2287, 2342
Bso31I GGTCTC 2 cut(s) 2362, 2482
BsoBI CYCGRG 2 cut(s) 697, 2419
Bsp119I TTCGAA 1 cut(s) 1427
Bsp1286I GDGCHC 3 cut(s) 897, 1400, 2516
Bsp13I TCCGGA 1 cut(s) 602
Bsp1407I TGTACA 1 cut(s) 1669
Bsp1720I GCTNAGC 1 cut(s) 896
Bsp19I CCATGG 1 cut(s) 2517
BspANI GGCC 7 cut(s) 14, 188, 979, 1389, 2067, 2287, 2342
BspCNI CTCAG 8 cut(s) 909, 976, 1194, 1291, 1342, 2032, 2043, 2377
BspEI TCCGGA 1 cut(s) 602
BspFNI CGCG 4 cut(s) 78, 132, 181, 2261
BspLI GGNNCC 7 cut(s) 148, 169, 205, 1047, 2239, 2353, 2515
BspMAI CTGCAG 1 cut(s) 559
BspMI ACCTGC 1 cut(s) 1204
BspPI GGATC 4 cut(s) 1276, 1840, 2346, 2359
BspT104I TTCGAA 1 cut(s) 1427
BspT107I GGYRCC 2 cut(s) 146, 2513
BspTNI GGTCTC 2 cut(s) 2362, 2482
BsrBI CCGCTC 2 cut(s) 1572, 2253
BsrDI GCAATG 1 cut(s) 1104
BsrFI RCCGGY 2 cut(s) 134, 200
BsrGI TGTACA 1 cut(s) 1669
BsrI ACTGG 7 cut(s) 69, 221, 412, 501, 828, 1226, 1866
BssAI RCCGGY 2 cut(s) 134, 200
BssECI CCNNGG 7 cut(s) 338, 697, 1061, 1305, 1890, 2419, 2517
BssNI GRCGYC 3 cut(s) 93, 147, 1793
BssT1I CCWWGG 3 cut(s) 338, 1061, 2517
Bst2UI CCWGG 2 cut(s) 1307, 1850
Bst4CI ACNGT 8 cut(s) 19, 362, 371, 424, 1229, 1894, 1995, 2454
Bst6I CTCTTC 5 cut(s) 61, 666, 917, 2322, 2480
BstACI GRCGYC 3 cut(s) 93, 147, 1793
BstAPI GCANNNNNTGC 1 cut(s) 665
BstAUI TGTACA 1 cut(s) 1669
BstBI TTCGAA 1 cut(s) 1427
BstDSI CCRYGG 2 cut(s) 1890, 2517
BstEII GGTNACC 1 cut(s) 1879
BstF5I GGATG 6 cut(s) 418, 498, 771, 1012, 1293, 2391
BstFNI CGCG 4 cut(s) 78, 132, 181, 2261
BstH2I RGCGCY 3 cut(s) 150, 1188, 1831
BstHHI GCGC 8 cut(s) 102, 134, 149, 1187, 1600, 1830, 2122, 2218
BstMCI CGRYCG 2 cut(s) 1922, 1994
BstNI CCWGG 2 cut(s) 1307, 1850
BstNSI RCATGY 3 cut(s) 554, 784, 1300
BstPI GGTNACC 1 cut(s) 1879
BstSCI CCNGG 8 cut(s) 696, 697, 1305, 1848, 1875, 1908, 2418, 2419
BstSFI CTRYAG 2 cut(s) 555, 654
BstSLI GKGCMC 1 cut(s) 2516
BstUI CGCG 4 cut(s) 78, 132, 181, 2261
BstV1I GCAGC 6 cut(s) 427, 541, 1216, 2185, 2276, 2279
BstV2I GAAGAC 1 cut(s) 614
BstX2I RGATCY 5 cut(s) 1268, 1418, 1845, 2351, 2475
BstYI RGATCY 5 cut(s) 1268, 1418, 1845, 2351, 2475
BsuI GTATCC 2 cut(s) 206, 717
BsuRI GGCC 7 cut(s) 14, 188, 979, 1389, 2067, 2287, 2342
BtgI CCRYGG 2 cut(s) 1890, 2517
BtgZI GCGATG 2 cut(s) 153, 2522
BtsCI GGATG 6 cut(s) 418, 498, 771, 1012, 1293, 2391
BtsI GCAGTG 2 cut(s) 1757, 1817
BtsIMutI CAGTG 4 cut(s) 405, 1757, 1817, 1873
BveI ACCTGC 1 cut(s) 1204
CfoI GCGC 8 cut(s) 102, 134, 149, 1187, 1600, 1830, 2122, 2218
Cfr10I RCCGGY 2 cut(s) 134, 200
Cfr13I GGNCC 4 cut(s) 186, 1018, 1169, 1177
Cfr9I CCCGGG 2 cut(s) 697, 2419
CseI GACGC 4 cut(s) 1360, 2190, 2267, 2452
Csp6I GTAC 3 cut(s) 1670, 2136, 2455
CviQI GTAC 3 cut(s) 1670, 2136, 2455
DinI GGCGCC 1 cut(s) 148
DraIII CACNNNGTG 1 cut(s) 183
DrdI GACNNNNNNGTC 2 cut(s) 170, 1910
DseDI GACNNNNNNGTC 2 cut(s) 170, 1910
EaeI YGGCCR 4 cut(s) 12, 1387, 2285, 2340
Eam1104I CTCTTC 5 cut(s) 61, 666, 917, 2322, 2480
EarI CTCTTC 5 cut(s) 61, 666, 917, 2322, 2480
EciI GGCGGA 1 cut(s) 52
Ecl136II GAGCTC 1 cut(s) 1398
Eco130I CCWWGG 3 cut(s) 338, 1061, 2517
Eco24I GRGCYC 2 cut(s) 897, 1400
Eco31I GGTCTC 2 cut(s) 2362, 2482
Eco47I GGWCC 3 cut(s) 1018, 1169, 1177
Eco47III AGCGCT 1 cut(s) 1186
Eco53kI GAGCTC 1 cut(s) 1398
Eco57I CTGAAG 4 cut(s) 651, 958, 1071, 1341
Eco88I CYCGRG 2 cut(s) 697, 2419
Eco91I GGTNACC 1 cut(s) 1879
EcoICRI GAGCTC 1 cut(s) 1398
EcoO109I RGGNCCY 1 cut(s) 1177
EcoO65I GGTNACC 1 cut(s) 1879
EcoRI GAATTC 1 cut(s) 1726
EcoRII CCWGG 2 cut(s) 1305, 1848
EcoT14I CCWWGG 3 cut(s) 338, 1061, 2517
EcoT22I ATGCAT 1 cut(s) 1116
EcoT38I GRGCYC 2 cut(s) 897, 1400
EgeI GGCGCC 1 cut(s) 148
EheI GGCGCC 1 cut(s) 148
ErhI CCWWGG 3 cut(s) 338, 1061, 2517
Esp3I CGTCTC 3 cut(s) 84, 1442, 2204
FalI AAGNNNNNCTT 2 cut(s) 1074, 1106
FaqI GGGAC 6 cut(s) 489, 574, 700, 940, 1238, 1859
FauI CCCGC 1 cut(s) 266
FauNDI CATATG 2 cut(s) 565, 859
FblI GTMKAC 2 cut(s) 1902, 1949
FokI GGATG 6 cut(s) 425, 505, 778, 1019, 1300, 2378
FriOI GRGCYC 2 cut(s) 897, 1400
FspBI CTAG 3 cut(s) 44, 711, 1731
FspI TGCGCA 1 cut(s) 2121
GlaI GCGC 8 cut(s) 101, 133, 148, 1186, 1599, 1829, 2121, 2217
GsaI CCCAGC 1 cut(s) 1203
GsuI CTGGAG 1 cut(s) 1849
HaeII RGCGCY 3 cut(s) 150, 1188, 1831
HaeIII GGCC 7 cut(s) 14, 188, 979, 1389, 2067, 2287, 2342
HgaI GACGC 4 cut(s) 1360, 2190, 2267, 2452
HhaI GCGC 8 cut(s) 102, 134, 149, 1187, 1600, 1830, 2122, 2218
Hin1I GRCGYC 3 cut(s) 93, 147, 1793
Hin6I GCGC 8 cut(s) 100, 132, 147, 1185, 1598, 1828, 2120, 2216
HinP1I GCGC 8 cut(s) 100, 132, 147, 1185, 1598, 1828, 2120, 2216
HincII GTYRAC 2 cut(s) 997, 1988
HindII GTYRAC 2 cut(s) 997, 1988
HindIII AAGCTT 3 cut(s) 114, 591, 1541
HphI GGTGA 8 cut(s) 383, 755, 896, 1050, 1255, 1537, 2150, 2156
Hpy166II GTNNAC 5 cut(s) 997, 1670, 1903, 1950, 1988
Hpy8I GTNNAC 5 cut(s) 997, 1670, 1903, 1950, 1988
Hpy99I CGWCG 9 cut(s) 23, 26, 112, 167, 176, 1628, 1792, 1795, 2261
HpyCH4III ACNGT 8 cut(s) 19, 362, 371, 424, 1229, 1894, 1995, 2454
HpyCH4IV ACGT 3 cut(s) 93, 1502, 1793
HpySE526I ACGT 3 cut(s) 93, 1502, 1793
Hsp92I GRCGYC 3 cut(s) 93, 147, 1793
HspAI GCGC 8 cut(s) 100, 132, 147, 1185, 1598, 1828, 2120, 2216
KasI GGCGCC 1 cut(s) 146
Kpn2I TCCGGA 1 cut(s) 602
KroI GCCGGC 2 cut(s) 134, 200
KroNI GCCGGC 2 cut(s) 136, 202
Lsp1109I GCAGC 6 cut(s) 427, 541, 1216, 2185, 2276, 2279
LweI GCATC 4 cut(s) 238, 483, 668, 997
MaeI CTAG 3 cut(s) 44, 711, 1731
MaeII ACGT 3 cut(s) 93, 1502, 1793
MaeIII GTNAC 6 cut(s) 418, 743, 776, 884, 1879, 1964
MbiI CCGCTC 2 cut(s) 1572, 2253
MflI RGATCY 5 cut(s) 1268, 1418, 1845, 2351, 2475
MhlI GDGCHC 3 cut(s) 897, 1400, 2516
MlsI TGGCCA 2 cut(s) 14, 2342
MluCI AATT 9 cut(s) 220, 257, 430, 850, 1085, 1423, 1534, 1726, 2091
MluNI TGGCCA 2 cut(s) 14, 2342
Mly113I GGCGCC 1 cut(s) 147
MlyI GAGTC 6 cut(s) 357, 1783, 1856, 1925, 2525, 2534
MmeI TCCRAC 3 cut(s) 90, 2419, 2508
Mox20I TGGCCA 2 cut(s) 14, 2342
Mph1103I ATGCAT 1 cut(s) 1116
MreI CGCCGGCG 1 cut(s) 134
MroI TCCGGA 1 cut(s) 602
MroNI GCCGGC 2 cut(s) 134, 200
MroXI GAANNNNTTC 3 cut(s) 971, 1504, 2095
MscI TGGCCA 2 cut(s) 14, 2342
MseI TTAA 4 cut(s) 426, 1646, 2346, 2406
MslI CAYNNNNRTG 6 cut(s) 372, 920, 956, 1119, 2169, 2519
Msp20I TGGCCA 2 cut(s) 14, 2342
MspA1I CMGCKG 2 cut(s) 1604, 2198
MspR9I CCNGG 8 cut(s) 698, 699, 1307, 1850, 1877, 1910, 2420, 2421
Mva1269I GAATGC 1 cut(s) 1116
MvaI CCWGG 2 cut(s) 1307, 1850
MvnI CGCG 4 cut(s) 78, 132, 181, 2261
NaeI GCCGGC 2 cut(s) 136, 202
NarI GGCGCC 1 cut(s) 147
NciI CCSGG 6 cut(s) 698, 699, 1877, 1910, 2420, 2421
NcoI CCATGG 1 cut(s) 2517
NdeI CATATG 2 cut(s) 565, 859
NgoMIV GCCGGC 2 cut(s) 134, 200
NlaIV GGNNCC 7 cut(s) 148, 169, 205, 1047, 2239, 2353, 2515
NmuCI GTSAC 3 cut(s) 743, 776, 884
NsbI TGCGCA 1 cut(s) 2121
NsiI ATGCAT 1 cut(s) 1116
NspI RCATGY 3 cut(s) 554, 784, 1300
NspV TTCGAA 1 cut(s) 1427
OliI CACNNNNGTG 1 cut(s) 2519
PaeI GCATGC 1 cut(s) 554
PcsI WCGNNNNNNNCGW 2 cut(s) 116, 168
PctI GAATGC 1 cut(s) 1116
PdiI GCCGGC 2 cut(s) 136, 202
PdmI GAANNNNTTC 3 cut(s) 971, 1504, 2095
PfeI GAWTC 6 cut(s) 599, 1158, 1413, 1516, 1585, 1819
PflFI GACNNNGTC 1 cut(s) 1792
PleI GAGTC 6 cut(s) 356, 1782, 1856, 1925, 2525, 2533
PluTI GGCGCC 1 cut(s) 150
PpsI GAGTC 6 cut(s) 356, 1782, 1856, 1925, 2525, 2533
PpuMI RGGWCCY 1 cut(s) 1177
PsiI TTATAA 1 cut(s) 384
Psp124BI GAGCTC 1 cut(s) 1400
Psp5II RGGWCCY 1 cut(s) 1177
Psp6I CCWGG 2 cut(s) 1305, 1848
PspEI GGTNACC 1 cut(s) 1879
PspFI CCCAGC 1 cut(s) 1199
PspGI CCWGG 2 cut(s) 1305, 1848
PspN4I GGNNCC 7 cut(s) 148, 169, 205, 1047, 2239, 2353, 2515
PspPI GGNCC 4 cut(s) 186, 1018, 1169, 1177
PspPPI RGGWCCY 1 cut(s) 1177
PstI CTGCAG 1 cut(s) 559
PsuI RGATCY 5 cut(s) 1268, 1418, 1845, 2351, 2475
PsyI GACNNNGTC 1 cut(s) 1792
RsaI GTAC 3 cut(s) 1671, 2137, 2456
RsaNI GTAC 3 cut(s) 1670, 2136, 2455
RseI CAYNNNNRTG 6 cut(s) 372, 920, 956, 1119, 2169, 2519
SacI GAGCTC 1 cut(s) 1400
SaqAI TTAA 4 cut(s) 426, 1646, 2346, 2406
Sau96I GGNCC 4 cut(s) 186, 1018, 1169, 1177
SchI GAGTC 6 cut(s) 357, 1783, 1856, 1925, 2525, 2534
ScrFI CCNGG 8 cut(s) 698, 699, 1307, 1850, 1877, 1910, 2420, 2421
SduI GDGCHC 3 cut(s) 897, 1400, 2516
SfaNI GCATC 4 cut(s) 238, 483, 668, 997
SfcI CTRYAG 2 cut(s) 555, 654
SfoI GGCGCC 1 cut(s) 148
SfuI TTCGAA 1 cut(s) 1427
SgrAI CRCCGGYG 1 cut(s) 134
SinI GGWCC 3 cut(s) 1018, 1169, 1177
SmaI CCCGGG 2 cut(s) 699, 2421
SmiMI CAYNNNNRTG 6 cut(s) 372, 920, 956, 1119, 2169, 2519
SmlI CTYRAG 2 cut(s) 1071, 1232
SmoI CTYRAG 2 cut(s) 1071, 1232
SphI GCATGC 1 cut(s) 554
Sse9I AATT 9 cut(s) 220, 257, 430, 850, 1085, 1423, 1534, 1726, 2091
SspDI GGCGCC 1 cut(s) 146
SspI AATATT 2 cut(s) 292, 1711
SspMI CTAG 3 cut(s) 44, 711, 1731
SstI GAGCTC 1 cut(s) 1400
StyD4I CCNGG 8 cut(s) 696, 697, 1305, 1848, 1875, 1908, 2418, 2419
StyI CCWWGG 3 cut(s) 338, 1061, 2517
TaaI ACNGT 8 cut(s) 19, 362, 371, 424, 1229, 1894, 1995, 2454
TaiI ACGT 3 cut(s) 96, 1505, 1796
TaqI TCGA 7 cut(s) 119, 1192, 1427, 1519, 1530, 1772, 1918
TaqII GACCGA 1 cut(s) 2506
TasI AATT 9 cut(s) 220, 257, 430, 850, 1085, 1423, 1534, 1726, 2091
TatI WGTACW 1 cut(s) 1669
TauI GCSGC 7 cut(s) 78, 2005, 2115, 2238, 2256, 2264, 2290
TfiI GAWTC 6 cut(s) 599, 1158, 1413, 1516, 1585, 1819
Tru1I TTAA 4 cut(s) 426, 1646, 2346, 2406
Tru9I TTAA 4 cut(s) 426, 1646, 2346, 2406
TscAI CASTG 4 cut(s) 412, 1764, 1817, 1873
TseFI GTSAC 3 cut(s) 743, 776, 884
TseI GCWGC 6 cut(s) 440, 554, 1204, 2198, 2264, 2267
Tsp45I GTSAC 3 cut(s) 743, 776, 884
TspGWI ACGGA 4 cut(s) 160, 1424, 1973, 2091
TspMI CCCGGG 2 cut(s) 697, 2419
TspRI CASTG 4 cut(s) 412, 1764, 1817, 1873
Tth111I GACNNNGTC 1 cut(s) 1792
VpaK11BI GGWCC 3 cut(s) 1018, 1169, 1177
XapI RAATTY 3 cut(s) 1085, 1534, 1726
XbaI TCTAGA 1 cut(s) 1730
XceI RCATGY 3 cut(s) 554, 784, 1300
XcmI CCANNNNNNNNNTGG 1 cut(s) 2280
XmaI CCCGGG 2 cut(s) 697, 2419
XmiI GTMKAC 2 cut(s) 1902, 1949
XmnI GAANNNNTTC 3 cut(s) 971, 1504, 2095
XspI CTAG 3 cut(s) 44, 711, 1731
ZraI GACGTC 2 cut(s) 94, 1794
Zsp2I ATGCAT 1 cut(s) 1116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.