RchiOBHm_Chr6g0310481

Vacuolar-processing enzyme-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0009785
Physical Location & Seq
Reverse (-)
67816187 .. 67820252
4066 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27921

Sequence Viewer

Length: 1635 bp
ATGTATCCCATATGGGACCCACGTATAGAAAAAGAAATCCCACGTGATTCACCACCAATAAATAAACAACAAACACTGCGACCTTCACCGAAACACAGCTCCACCAATCAAAAATATCCTATCCCACTTTCGCCGGAGACCGCCATGACTCGGTTGGCCACCGCCGTCGCCGTCCTTCTTTTCTTCCTAGTCTCCCTCTCTTCTTCCGCCACCAGTAGCCGCGACCTGATCGGAGACGTCTTGCGCTTGCCGTCGGAAGCTTCGAGGTTCTTCCGCGCCGGCGATGGTGGCGCCGATCAGCAAGACGACGGCTCCGTCGGCACGCGGTGGGCCGTTTTGATCGCCGGCTCCAATGGATACTGGAATTACAGGCATCAGGCTGATATATGTCATGCCTATCAATTATTGAAGAAAGGCGGGCTGAAAGATGAAAATATTGTTGTATTTATGTATGATGATATTGCTTACAATGAAGAGAACCCTAGGCAAGGAGTCATCATCAACAGTCCTCACGGTGATGATGTTTATAAAGGAGTTCCGAAGGATTACACTGGGGATGATGTTACTGTTAATAATTTCTTTGCTGCTATTCTTGGAAACAAAACTGCTATTACCGGGGGTAGTGGGAAAGTTGTGGACAGTGGTCCGAATGATCACATCTTCATATACTACTCCGATCATGGTGGTCCTGGAGTACTTGGGATGCCTACCAGTCCCTATATTTATGCAGACAGGCTCATAGAAGTTCTAAAGAAGAAGCATGCTGCAGGGACATATGAAAGTTTGGTATTTTATCTTGAAGCTTGTGAATCCGGAAGTATCTTTGAAGGTCTTCTTCCTGAAGGTTTGAATATCTTTGCAACTACAGCATCAAATGCAGAAGAGAGCAGTTGGGGGACTTACTGCCCGGGAGAGTATCCTAGTCCTCCCCCAGAATATGAAACCTGTTTGGGTGACTTGTATAGTGTTGCTTGGATGGAAGATAGTGACGTACACAACTTGAAGTCAGAAACTTTGCACCAGCAGTATGAACTGGTCAAATCAAGGACAGCGAGTGATAATTCTGCTTATGGTTCTCATGTCATGCAATATGGCGACATAGGGCTCAGCAAGAACAACCTCTTCATGTATATGGGGACGAATCCTGCAAATGATAACTACACTTTCATGCCTCAGAACTTCTTCAGGCCATCTTCAAAAGCTGTCAACCAGAGGGATGCTGATCTGGTCCATTTCTGGGATAAGTTCCGCAAGGCTCCTGAAGGCTCACCAAGGAAGGCTCAAGCCCAAAAAGAATTTCTTGAAGCAATGTCTCACAGAATGCATATAGATGAAAGCATGAAACTTATTGGGAAACTCCTGTTTGGAATCAAAAAAGGTCCAGAGATCCTCAGCGCTGTTCGACCTGCTGGGCAGCCACTTGTTGATGACTGGGACTGTCTTAAGACAATGGTGAGGAGTTTTGAGACGTATTGCGGATCTCTATCTCAGTATGGGATGAAACACATGCGTTCCCTGGCAAACATCTGCAACGCTGGAATGACGAAGGAGCAGATGGCTGAGGCTTCAGCCCAAGCCTGCGTCAATGTTCCTTCTGGCCGATGGAGCTCTCTGCACAGGGGATTCAGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04612 map04142 map04612
Pfam Domains
Protein Families
Peptidase_C13

Protein Analysis

544

Amino Acids

60.26

Weight (kDa)

5.78

Isoelectric Point (pI)

49.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C13 PF01650 109 - 380 3e-118 Peptidase C13 family
Legum_prodom PF20985 432 - 527 4.8e-43 Legumain, prodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009785)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G25940 AT4G32940
fragaria_vesca FvH4_2g32190 FvH4_2g32190 FvH4_2g32190 FvH4_2g32190
malus_domestica MD08G1073500.v1.1
prunus_persica Prupe.1G415000_v2.0.a1 Prupe.1G415000_v2.0.a1
pyrus_communis pycom08g06090 pycom15g05790
rosa_chinensis RchiOBHm_Chr6g0310481
rosa_laevigata RLG00000010479
rosa_multiflora Rmu_sc0035020.1_g000006
rosa_roxburghii Rroxscaffold_7G00158490
rosa_rugosa Rorug06G0380700
rosa_samantha Rh6AG494700 Rh6BG504400 Rh6CG509600 Rh6DG495600
rosa_wichuraiana Rw6G043030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 528
AasI GACNNNNNNGTC 1 cut(s) 314
AatII GACGTC 1 cut(s) 240
Acc36I ACCTGC 1 cut(s) 1414
AccB1I GGYRCC 1 cut(s) 290
AccII CGCG 3 cut(s) 222, 276, 325
AccIII TCCGGA 1 cut(s) 812
AciI CCGC 9 cut(s) 141, 162, 207, 220, 274, 325, 417, 1249, 1476
AclWI GGATC 2 cut(s) 1381, 1486
AcoI YGGCCR 2 cut(s) 156, 1597
AcsI RAATTY 1 cut(s) 1295
AcuI CTGAAG 4 cut(s) 861, 1168, 1281, 1551
AcvI CACGTG 1 cut(s) 44
AcyI GRCGYC 2 cut(s) 237, 291
AdeI CACNNNGTG 1 cut(s) 327
AfaI GTAC 2 cut(s) 696, 993
AfeI AGCGCT 1 cut(s) 1396
AfiI CCNNNNNNNGG 3 cut(s) 150, 488, 1237
AflII CTTAAG 1 cut(s) 1442
AgsI TTSAA 7 cut(s) 409, 800, 827, 850, 1003, 1197, 1304
AjnI CCWGG 2 cut(s) 688, 1515
AjuI GAANNNNNNNTTGG 2 cut(s) 1281, 1313
AluBI AGCT 5 cut(s) 99, 260, 803, 1202, 1608
AluI AGCT 5 cut(s) 99, 260, 803, 1202, 1608
Alw21I GWGCWC 1 cut(s) 1610
Alw26I GTCTC 5 cut(s) 131, 196, 228, 1317, 1460
AlwI GGATC 2 cut(s) 1381, 1486
Ama87I CYCGRG 1 cut(s) 907
Aor13HI TCCGGA 1 cut(s) 812
Aor51HI AGCGCT 1 cut(s) 1396
AoxI GGCC 4 cut(s) 156, 330, 1187, 1597
ApeKI GCWGC 3 cut(s) 584, 764, 1414
ApoI RAATTY 1 cut(s) 1295
Asp700I GAANNNNTTC 2 cut(s) 831, 1181
AspA2I CCTAGG 1 cut(s) 482
AspLEI GCGC 4 cut(s) 246, 278, 293, 1397
AspS9I GGNCC 6 cut(s) 16, 330, 644, 686, 1228, 1379
AsuC2I CCSGG 3 cut(s) 616, 908, 909
AsuHPI GGTGA 6 cut(s) 42, 78, 527, 965, 1260, 1465
AvaI CYCGRG 1 cut(s) 907
AvaII GGWCC 5 cut(s) 16, 644, 686, 1228, 1379
AvrII CCTAGG 1 cut(s) 482
BalI TGGCCA 1 cut(s) 158
BanI GGYRCC 1 cut(s) 290
BanII GRGCYC 2 cut(s) 1107, 1610
BbrPI CACGTG 1 cut(s) 44
BbsI GAAGAC 1 cut(s) 824
Bbv12I GWGCWC 1 cut(s) 1610
BbvCI CCTCAGC 2 cut(s) 1391, 1560
BbvI GCAGC 3 cut(s) 571, 751, 1426
BccI CCATC 5 cut(s) 278, 970, 1198, 1549, 1596
BceAI ACGGC 5 cut(s) 149, 155, 235, 317, 325
BciT130I CCWGG 2 cut(s) 690, 1517
BciVI GTATCC 3 cut(s) 15, 350, 927
BclI TGATCA 1 cut(s) 652
BcnI CCSGG 3 cut(s) 616, 908, 909
BcoDI GTCTC 5 cut(s) 131, 196, 228, 1317, 1460
BfaI CTAG 3 cut(s) 188, 483, 921
BfmI CTRYAG 2 cut(s) 765, 864
BfoI RGCGCY 2 cut(s) 294, 1398
BfrI CTTAAG 1 cut(s) 1442
BfuAI ACCTGC 1 cut(s) 1414
BfuI GTATCC 3 cut(s) 15, 350, 927
BisI GCNGC 4 cut(s) 220, 585, 765, 1415
BlnI CCTAGG 1 cut(s) 482
BlpI GCTNAGC 1 cut(s) 1106
BlsI GCNGC 4 cut(s) 221, 586, 766, 1416
BmcAI AGTACT 1 cut(s) 696
Bme1390I CCNGG 5 cut(s) 616, 690, 908, 909, 1517
Bme18I GGWCC 5 cut(s) 16, 644, 686, 1228, 1379
BmeT110I CYCGRG 1 cut(s) 907
BmgT120I GGNCC 6 cut(s) 16, 330, 644, 686, 1228, 1379
BmiI GGNNCC 6 cut(s) 17, 18, 292, 313, 349, 1257
BmrFI CCNGG 5 cut(s) 616, 690, 908, 909, 1517
BmrI ACTGGG 2 cut(s) 561, 1441
BmsI GCATC 4 cut(s) 382, 693, 878, 1207
BmuI ACTGGG 2 cut(s) 561, 1441
BoxI GACNNNNGTC 1 cut(s) 642
BpiI GAAGAC 1 cut(s) 824
BpmI CTGGAG 1 cut(s) 711
Bpu10I CCTNAGC 2 cut(s) 1391, 1560
Bpu1102I GCTNAGC 1 cut(s) 1106
BpuEI CTTGAG 1 cut(s) 1266
BpuMI CCSGG 3 cut(s) 616, 908, 909
BsaAI YACGTR 2 cut(s) 23, 44
BsaBI GATNNNNATC 2 cut(s) 1221, 1483
BsaHI GRCGYC 2 cut(s) 237, 291
BsaI GGTCTC 1 cut(s) 131
BsaJI CCNNGG 5 cut(s) 482, 615, 907, 1271, 1515
BsaWI WCCGGW 1 cut(s) 812
BsaXI ACNNNNNCTCC 2 cut(s) 296, 326
Bsc4I CCNNNNNNNGG 3 cut(s) 150, 488, 1237
Bse118I RCCGGY 2 cut(s) 278, 344
Bse1I ACTGG 6 cut(s) 213, 365, 556, 711, 1038, 1436
Bse3DI GCAATG 1 cut(s) 1314
Bse8I GATNNNNATC 2 cut(s) 1221, 1483
BseAI TCCGGA 1 cut(s) 812
BseBI CCWGG 2 cut(s) 690, 1517
BseDI CCNNGG 5 cut(s) 482, 615, 907, 1271, 1515
BseGI GGATG 5 cut(s) 562, 708, 981, 1222, 1503
BseJI GATNNNNATC 2 cut(s) 1221, 1483
BseLI CCNNNNNNNGG 3 cut(s) 150, 488, 1237
BseMI GCAATG 1 cut(s) 1314
BseMII CTCAG 5 cut(s) 1120, 1187, 1405, 1502, 1551
BseNI ACTGG 6 cut(s) 213, 365, 556, 711, 1038, 1436
BseRI GAGGAG 1 cut(s) 1471
BseXI GCAGC 3 cut(s) 571, 751, 1426
BseYI CCCAGC 1 cut(s) 1409
BsgI GTGCAG 1 cut(s) 1598
Bsh1236I CGCG 3 cut(s) 222, 276, 325
BshFI GGCC 4 cut(s) 158, 332, 1189, 1599
BshNI GGYRCC 1 cut(s) 290
BsiHKAI GWGCWC 1 cut(s) 1610
BsiHKCI CYCGRG 1 cut(s) 907
BsiSI CCGG 6 cut(s) 134, 279, 345, 615, 813, 908
BslFI GGGAC 6 cut(s) 29, 699, 784, 910, 1150, 1448
BslI CCNNNNNNNGG 3 cut(s) 150, 488, 1237
BsmAI GTCTC 5 cut(s) 131, 196, 228, 1317, 1460
BsmBI CGTCTC 2 cut(s) 228, 1460
BsmFI GGGAC 6 cut(s) 29, 699, 784, 910, 1150, 1448
BsmI GAATGC 1 cut(s) 1326
BsnI GGCC 4 cut(s) 158, 332, 1189, 1599
Bso31I GGTCTC 1 cut(s) 131
BsoBI CYCGRG 1 cut(s) 907
Bsp1286I GDGCHC 2 cut(s) 1107, 1610
Bsp13I TCCGGA 1 cut(s) 812
Bsp143I GATC 8 cut(s) 228, 295, 339, 652, 676, 1222, 1386, 1478
Bsp1720I GCTNAGC 1 cut(s) 1106
BspACI CCGC 9 cut(s) 141, 162, 207, 220, 274, 325, 417, 1249, 1476
BspANI GGCC 4 cut(s) 158, 332, 1189, 1599
BspCNI CTCAG 5 cut(s) 1119, 1186, 1404, 1501, 1552
BspEI TCCGGA 1 cut(s) 812
BspFNI CGCG 3 cut(s) 222, 276, 325
BspLI GGNNCC 6 cut(s) 17, 18, 292, 313, 349, 1257
BspMAI CTGCAG 1 cut(s) 769
BspMI ACCTGC 1 cut(s) 1414
BspPI GGATC 2 cut(s) 1381, 1486
BspT107I GGYRCC 1 cut(s) 290
BspTI CTTAAG 1 cut(s) 1442
BspTNI GGTCTC 1 cut(s) 131
BsrDI GCAATG 1 cut(s) 1314
BsrFI RCCGGY 2 cut(s) 278, 344
BsrI ACTGG 6 cut(s) 213, 365, 556, 711, 1038, 1436
BssAI RCCGGY 2 cut(s) 278, 344
BssECI CCNNGG 5 cut(s) 482, 615, 907, 1271, 1515
BssMI GATC 8 cut(s) 228, 295, 339, 652, 676, 1222, 1386, 1478
BssNI GRCGYC 2 cut(s) 237, 291
BssT1I CCWWGG 2 cut(s) 482, 1271
Bst2UI CCWGG 2 cut(s) 690, 1517
Bst4CI ACNGT 5 cut(s) 506, 515, 568, 641, 1439
Bst6I CTCTTC 4 cut(s) 205, 468, 876, 1127
BstACI GRCGYC 2 cut(s) 237, 291
BstAFI CTTAAG 1 cut(s) 1442
BstAPI GCANNNNNTGC 1 cut(s) 875
BstBAI YACGTR 2 cut(s) 23, 44
BstC8I GCNNGC 7 cut(s) 248, 280, 323, 346, 419, 762, 1579
BstDEI CTNAG 5 cut(s) 1106, 1173, 1391, 1488, 1560
BstENI CCTNNNNNAGG 1 cut(s) 486
BstF5I GGATG 5 cut(s) 562, 708, 981, 1222, 1503
BstFNI CGCG 3 cut(s) 222, 276, 325
BstH2I RGCGCY 2 cut(s) 294, 1398
BstHHI GCGC 4 cut(s) 246, 278, 293, 1397
BstKTI GATC 8 cut(s) 231, 298, 342, 655, 679, 1225, 1389, 1481
BstMAI GTCTC 5 cut(s) 131, 196, 228, 1317, 1460
BstMBI GATC 8 cut(s) 228, 295, 339, 652, 676, 1222, 1386, 1478
BstMWI GCNNNNNNNGC 5 cut(s) 288, 318, 866, 875, 1605
BstNI CCWGG 2 cut(s) 690, 1517
BstNSI RCATGY 2 cut(s) 764, 1510
BstPAI GACNNNNGTC 1 cut(s) 642
BstSCI CCNGG 5 cut(s) 614, 688, 906, 907, 1515
BstSFI CTRYAG 2 cut(s) 765, 864
BstUI CGCG 3 cut(s) 222, 276, 325
BstV1I GCAGC 3 cut(s) 571, 751, 1426
BstV2I GAAGAC 1 cut(s) 824
BstX2I RGATCY 2 cut(s) 1386, 1478
BstYI RGATCY 2 cut(s) 1386, 1478
BsuI GTATCC 3 cut(s) 15, 350, 927
BsuRI GGCC 4 cut(s) 158, 332, 1189, 1599
BtgZI GCGATG 1 cut(s) 297
BtsCI GGATG 5 cut(s) 562, 708, 981, 1222, 1503
BtsI GCAGTG 1 cut(s) 74
BtsIMutI CAGTG 4 cut(s) 74, 549, 646, 1633
BveI ACCTGC 1 cut(s) 1414
Cac8I GCNNGC 7 cut(s) 248, 280, 323, 346, 419, 762, 1579
CfoI GCGC 4 cut(s) 246, 278, 293, 1397
Cfr10I RCCGGY 2 cut(s) 278, 344
Cfr13I GGNCC 6 cut(s) 16, 330, 644, 686, 1228, 1379
Cfr9I CCCGGG 1 cut(s) 907
CseI GACGC 1 cut(s) 1570
Csp6I GTAC 2 cut(s) 695, 992
CviQI GTAC 2 cut(s) 695, 992
DdeI CTNAG 5 cut(s) 1106, 1173, 1391, 1488, 1560
DinI GGCGCC 1 cut(s) 292
DpnI GATC 8 cut(s) 230, 297, 341, 654, 678, 1224, 1388, 1480
DpnII GATC 8 cut(s) 228, 295, 339, 652, 676, 1222, 1386, 1478
DraIII CACNNNGTG 1 cut(s) 327
DrdI GACNNNNNNGTC 1 cut(s) 314
DseDI GACNNNNNNGTC 1 cut(s) 314
EaeI YGGCCR 2 cut(s) 156, 1597
Eam1104I CTCTTC 4 cut(s) 205, 468, 876, 1127
EarI CTCTTC 4 cut(s) 205, 468, 876, 1127
EciI GGCGGA 1 cut(s) 196
Ecl136II GAGCTC 1 cut(s) 1608
Eco130I CCWWGG 2 cut(s) 482, 1271
Eco24I GRGCYC 2 cut(s) 1107, 1610
Eco31I GGTCTC 1 cut(s) 131
Eco47I GGWCC 5 cut(s) 16, 644, 686, 1228, 1379
Eco47III AGCGCT 1 cut(s) 1396
Eco53kI GAGCTC 1 cut(s) 1608
Eco57I CTGAAG 4 cut(s) 861, 1168, 1281, 1551
Eco72I CACGTG 1 cut(s) 44
Eco88I CYCGRG 1 cut(s) 907
EcoICRI GAGCTC 1 cut(s) 1608
EcoNI CCTNNNNNAGG 1 cut(s) 486
EcoO109I RGGNCCY 1 cut(s) 16
EcoRII CCWGG 2 cut(s) 688, 1515
EcoT14I CCWWGG 2 cut(s) 482, 1271
EcoT22I ATGCAT 1 cut(s) 1326
EcoT38I GRGCYC 2 cut(s) 1107, 1610
EgeI GGCGCC 1 cut(s) 292
EheI GGCGCC 1 cut(s) 292
ErhI CCWWGG 2 cut(s) 482, 1271
Esp3I CGTCTC 2 cut(s) 228, 1460
FalI AAGNNNNNCTT 4 cut(s) 819, 851, 1284, 1316
FaqI GGGAC 6 cut(s) 29, 699, 784, 910, 1150, 1448
FauI CCCGC 1 cut(s) 410
FauNDI CATATG 2 cut(s) 11, 775
FbaI TGATCA 1 cut(s) 652
Fnu4HI GCNGC 4 cut(s) 220, 585, 765, 1415
FokI GGATG 5 cut(s) 569, 715, 988, 1229, 1510
FriOI GRGCYC 2 cut(s) 1107, 1610
Fsp4HI GCNGC 4 cut(s) 220, 585, 765, 1415
FspBI CTAG 3 cut(s) 188, 483, 921
GlaI GCGC 4 cut(s) 245, 277, 292, 1396
GluI GCNGC 4 cut(s) 220, 585, 765, 1415
GsaI CCCAGC 1 cut(s) 1413
GsuI CTGGAG 1 cut(s) 711
HaeII RGCGCY 2 cut(s) 294, 1398
HaeIII GGCC 4 cut(s) 158, 332, 1189, 1599
HapII CCGG 6 cut(s) 134, 279, 345, 615, 813, 908
HgaI GACGC 1 cut(s) 1570
HhaI GCGC 4 cut(s) 246, 278, 293, 1397
Hin1I GRCGYC 2 cut(s) 237, 291
Hin6I GCGC 4 cut(s) 244, 276, 291, 1395
HinP1I GCGC 4 cut(s) 244, 276, 291, 1395
HincII GTYRAC 1 cut(s) 1207
HindII GTYRAC 1 cut(s) 1207
HindIII AAGCTT 2 cut(s) 258, 801
HinfI GANTC 7 cut(s) 47, 148, 492, 809, 1141, 1368, 1623
HpaII CCGG 6 cut(s) 134, 279, 345, 615, 813, 908
HphI GGTGA 6 cut(s) 42, 78, 527, 965, 1260, 1465
Hpy166II GTNNAC 3 cut(s) 637, 994, 1207
Hpy188I TCNGA 7 cut(s) 233, 256, 540, 648, 676, 1009, 1176
Hpy188III TCNNGA 6 cut(s) 797, 813, 839, 1259, 1301, 1382
Hpy8I GTNNAC 3 cut(s) 637, 994, 1207
Hpy99I CGWCG 4 cut(s) 170, 256, 311, 320
HpyAV CCTTC 9 cut(s) 93, 185, 535, 821, 836, 1256, 1270, 1540, 1602
HpyCH4III ACNGT 5 cut(s) 506, 515, 568, 641, 1439
HpyCH4IV ACGT 5 cut(s) 22, 43, 237, 990, 1469
HpyF10VI GCNNNNNNNGC 5 cut(s) 288, 318, 866, 875, 1605
HpyF3I CTNAG 5 cut(s) 1106, 1173, 1391, 1488, 1560
HpySE526I ACGT 5 cut(s) 22, 43, 237, 990, 1469
Hsp92I GRCGYC 2 cut(s) 237, 291
HspAI GCGC 4 cut(s) 244, 276, 291, 1395
KasI GGCGCC 1 cut(s) 290
KflI GGGWCCC 1 cut(s) 16
Kpn2I TCCGGA 1 cut(s) 812
KroI GCCGGC 2 cut(s) 278, 344
KroNI GCCGGC 2 cut(s) 280, 346
Ksp22I TGATCA 1 cut(s) 652
Kzo9I GATC 8 cut(s) 228, 295, 339, 652, 676, 1222, 1386, 1478
LmnI GCTCC 6 cut(s) 104, 317, 353, 1261, 1549, 1605
Lsp1109I GCAGC 3 cut(s) 571, 751, 1426
LweI GCATC 4 cut(s) 382, 693, 878, 1207
MaeI CTAG 3 cut(s) 188, 483, 921
MaeII ACGT 5 cut(s) 22, 43, 237, 990, 1469
MaeIII GTNAC 3 cut(s) 562, 953, 986
MalI GATC 8 cut(s) 230, 297, 341, 654, 678, 1224, 1388, 1480
MboI GATC 8 cut(s) 228, 295, 339, 652, 676, 1222, 1386, 1478
MflI RGATCY 2 cut(s) 1386, 1478
MhlI GDGCHC 2 cut(s) 1107, 1610
MlsI TGGCCA 1 cut(s) 158
MluCI AATT 5 cut(s) 364, 401, 574, 1060, 1295
MluNI TGGCCA 1 cut(s) 158
Mly113I GGCGCC 1 cut(s) 291
MlyI GAGTC 2 cut(s) 142, 501
MmeI TCCRAC 1 cut(s) 234
Mox20I TGGCCA 1 cut(s) 158
Mph1103I ATGCAT 1 cut(s) 1326
MreI CGCCGGCG 1 cut(s) 278
MroI TCCGGA 1 cut(s) 812
MroNI GCCGGC 2 cut(s) 278, 344
MroXI GAANNNNTTC 2 cut(s) 831, 1181
MscI TGGCCA 1 cut(s) 158
MseI TTAA 2 cut(s) 570, 1443
MslI CAYNNNNRTG 4 cut(s) 516, 1130, 1166, 1329
Msp20I TGGCCA 1 cut(s) 158
MspCI CTTAAG 1 cut(s) 1442
MspI CCGG 6 cut(s) 134, 279, 345, 615, 813, 908
MspR9I CCNGG 5 cut(s) 616, 690, 908, 909, 1517
Mva1269I GAATGC 1 cut(s) 1326
MvaI CCWGG 2 cut(s) 690, 1517
MvnI CGCG 3 cut(s) 222, 276, 325
MwoI GCNNNNNNNGC 5 cut(s) 288, 318, 866, 875, 1605
NaeI GCCGGC 2 cut(s) 280, 346
NarI GGCGCC 1 cut(s) 291
NciI CCSGG 3 cut(s) 616, 908, 909
NdeI CATATG 2 cut(s) 11, 775
NdeII GATC 8 cut(s) 228, 295, 339, 652, 676, 1222, 1386, 1478
NgoMIV GCCGGC 2 cut(s) 278, 344
NlaIV GGNNCC 6 cut(s) 17, 18, 292, 313, 349, 1257
NmuCI GTSAC 2 cut(s) 953, 986
NsiI ATGCAT 1 cut(s) 1326
NspI RCATGY 2 cut(s) 764, 1510
PaeI GCATGC 1 cut(s) 764
PcsI WCGNNNNNNNCGW 2 cut(s) 260, 312
PctI GAATGC 1 cut(s) 1326
PdiI GCCGGC 2 cut(s) 280, 346
PdmI GAANNNNTTC 2 cut(s) 831, 1181
PfeI GAWTC 5 cut(s) 47, 809, 1141, 1368, 1623
PfoI TCCNGGA 1 cut(s) 688
PkrI GCNGC 4 cut(s) 221, 586, 766, 1416
PleI GAGTC 2 cut(s) 142, 500
PluTI GGCGCC 1 cut(s) 294
PmaCI CACGTG 1 cut(s) 44
PmlI CACGTG 1 cut(s) 44
PpsI GAGTC 2 cut(s) 142, 500
Ppu21I YACGTR 2 cut(s) 23, 44
PpuMI RGGWCCY 1 cut(s) 16
PshAI GACNNNNGTC 1 cut(s) 642
PsiI TTATAA 1 cut(s) 528
Psp124BI GAGCTC 1 cut(s) 1610
Psp5II RGGWCCY 1 cut(s) 16
Psp6I CCWGG 2 cut(s) 688, 1515
PspCI CACGTG 1 cut(s) 44
PspFI CCCAGC 1 cut(s) 1409
PspGI CCWGG 2 cut(s) 688, 1515
PspN4I GGNNCC 6 cut(s) 17, 18, 292, 313, 349, 1257
PspPI GGNCC 6 cut(s) 16, 330, 644, 686, 1228, 1379
PspPPI RGGWCCY 1 cut(s) 16
PstI CTGCAG 1 cut(s) 769
PsuI RGATCY 2 cut(s) 1386, 1478
RsaI GTAC 2 cut(s) 696, 993
RsaNI GTAC 2 cut(s) 695, 992
RseI CAYNNNNRTG 4 cut(s) 516, 1130, 1166, 1329
SacI GAGCTC 1 cut(s) 1610
SaqAI TTAA 2 cut(s) 570, 1443
SatI GCNGC 4 cut(s) 220, 585, 765, 1415
Sau3AI GATC 8 cut(s) 228, 295, 339, 652, 676, 1222, 1386, 1478
Sau96I GGNCC 6 cut(s) 16, 330, 644, 686, 1228, 1379
ScaI AGTACT 1 cut(s) 696
SchI GAGTC 2 cut(s) 142, 501
ScrFI CCNGG 5 cut(s) 616, 690, 908, 909, 1517
SduI GDGCHC 2 cut(s) 1107, 1610
SfaNI GCATC 4 cut(s) 382, 693, 878, 1207
SfcI CTRYAG 2 cut(s) 765, 864
SfoI GGCGCC 1 cut(s) 292
SgrAI CRCCGGYG 1 cut(s) 278
SinI GGWCC 5 cut(s) 16, 644, 686, 1228, 1379
SmaI CCCGGG 1 cut(s) 909
SmiMI CAYNNNNRTG 4 cut(s) 516, 1130, 1166, 1329
SmlI CTYRAG 2 cut(s) 1281, 1442
SmoI CTYRAG 2 cut(s) 1281, 1442
SphI GCATGC 1 cut(s) 764
Sse9I AATT 5 cut(s) 364, 401, 574, 1060, 1295
SsiI CCGC 9 cut(s) 141, 162, 207, 220, 274, 325, 417, 1249, 1476
SspDI GGCGCC 1 cut(s) 290
SspI AATATT 1 cut(s) 436
SspMI CTAG 3 cut(s) 188, 483, 921
SstI GAGCTC 1 cut(s) 1610
StyD4I CCNGG 5 cut(s) 614, 688, 906, 907, 1515
StyI CCWWGG 2 cut(s) 482, 1271
TaaI ACNGT 5 cut(s) 506, 515, 568, 641, 1439
TaiI ACGT 5 cut(s) 25, 46, 240, 993, 1472
TaqI TCGA 2 cut(s) 263, 1402
TasI AATT 5 cut(s) 364, 401, 574, 1060, 1295
TatI WGTACW 1 cut(s) 694
TauI GCSGC 1 cut(s) 222
TfiI GAWTC 5 cut(s) 47, 809, 1141, 1368, 1623
Tru1I TTAA 2 cut(s) 570, 1443
Tru9I TTAA 2 cut(s) 570, 1443
TscAI CASTG 4 cut(s) 81, 556, 646, 1633
TseFI GTSAC 2 cut(s) 953, 986
TseI GCWGC 3 cut(s) 584, 764, 1414
Tsp45I GTSAC 2 cut(s) 953, 986
TspGWI ACGGA 1 cut(s) 304
TspMI CCCGGG 1 cut(s) 907
TspRI CASTG 4 cut(s) 81, 556, 646, 1633
Vha464I CTTAAG 1 cut(s) 1442
VpaK11BI GGWCC 5 cut(s) 16, 644, 686, 1228, 1379
XagI CCTNNNNNAGG 1 cut(s) 486
XapI RAATTY 1 cut(s) 1295
XceI RCATGY 2 cut(s) 764, 1510
XcmI CCANNNNNNNNNTGG 1 cut(s) 151
XmaI CCCGGG 1 cut(s) 907
XmaJI CCTAGG 1 cut(s) 482
XmnI GAANNNNTTC 2 cut(s) 831, 1181
XspI CTAG 3 cut(s) 188, 483, 921
ZraI GACGTC 1 cut(s) 238
ZrmI AGTACT 1 cut(s) 696
Zsp2I ATGCAT 1 cut(s) 1326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.