RLG00000010479

Vacuolar-processing enzyme-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0009785
Physical Location & Seq
Forward (+)
1987896 .. 1991532
3637 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010479

Sequence Viewer

Length: 1491 bp
ATGACTCGTTTGGCCACTGTCGTCGCCGTCCTTCTTTTCTTCCTAGTCTCCCTCTCTTCTTCGGCCACCAGTAGCCGCGACCTGATCGGAGACGTCTTGCGCTTGCCGTCCGAAGCTTCGAGGTTCTTCCGCCCCGGCGATGGTGGCGCCGATCATCAAGACGACGGCTCCGTCGGCACGCGGTGGGCCGTTTTGATCGCCGGCTCCAATGGATACTGGAATTACAGGCATCAGGCTGATGTATGCCATGCCTATCAATTATTGAAGAAGGGAGGGCTGAAAGATGAAAATATTGTTGTATTTATGTATGATGATATTGCTTACAATGAAGAGAACCCTAGGCAAGGAGTCATAATCAACAGTCCTCACGGTGATGATGTTTATAAAGGAGTTCCTAAGGATTACACTGGGGGTGATGTTACTGTTAATAATTTCTTTGCTGCTATTCTTGGAAACAAAACTGCTATTACCGGGGGTAGTGGGAAAGTTGTGGACAGTGGTCCGAATGATCACATCTTCATATACTACTCCGATCATGGTGGTCCTGGAGTACTTGGGATGCCTACCAGTCCCTATATTTATGCAGACAGGCTCATAGAAGTTCTAAAGAAGAAGCATGCTGCAGGGACATATGAAAGTTTGGTATTTTATCTTGAAGCTTGTGAATCCGGAAGTATCTTTGAGGGTCTTCTTCCTGAAGGTTTGAATATCTTTGCAACTACAGCATCAAATGCAGAAGAGAGCAGTTGGGGGACTTACTGCCCGGGAGAGTATCCTAGTCCTCCCCCAGAATATGAAACCTGTTTGGGTGACTTGTATAGTGTTGCTTGGATGGAAGATAGTGACGTACACAACTTGAAGTCAGAAACTTTGCACCAGCAGTATGAACTGGTCAAATCAAGGACAGCGAATGATAATTCTGCTTTTGGTTCTCATGTCATGCAATATGGCGACATAGGGCTCAGCAAGAACAACCTCTTCATGTATATGGGGACAAATCCTGCAAATGATAACTACACTTTCATGCCTCAGAACTTCTTCAGGCCATCTTCAAAAGCTGTGAACCAGAGGGATGCTGATCTGGTCCATTTCTGGGATAAGTTCCGCAAGGCTCCTGAAGGCTCACCAAGGAAAGCTCAAGCCCAAAAAGAATTTCTTGAAGCAATGTCTCACAGAATGCATATAGATGAAAGCATGAAACTTATTGGGAAACTCCTGTTTGGAATCAAAAAAGGTCCAGAGGTCCTCAGCGCTGTTCGACCTGCTGGGCAGCCACTTGTTGATGACTGGGACTGTCTTAAGACAATGGTGAGGAGTTTTGAGACGTATTGCGGATCTCTATCTCAGTATGGGATGAAACACATGCGTTCCCTGGCAAACATCTGCAACGCTGGAATGACGAAGGAGCAGATGGCCGAGGCTTCAGCCCAAGCCTGCGTCAATGTTCCTTCTGGCCGATGGAGCTCTCTGCACAGGGGATTCAGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04612 map04142 map04612
Pfam Domains
Protein Families
Peptidase_C13

Protein Analysis

497

Amino Acids

54.68

Weight (kDa)

5.69

Isoelectric Point (pI)

45.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C13 PF01650 61 - 332 9.2e-118 Peptidase C13 family
Legum_prodom PF20985 384 - 479 3.4e-43 Legumain, prodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009785)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G25940 AT4G32940
fragaria_vesca FvH4_2g32190 FvH4_2g32190 FvH4_2g32190 FvH4_2g32190
malus_domestica MD08G1073500.v1.1
prunus_persica Prupe.1G415000_v2.0.a1 Prupe.1G415000_v2.0.a1
pyrus_communis pycom08g06090 pycom15g05790
rosa_chinensis RchiOBHm_Chr6g0310481
rosa_laevigata RLG00000010479
rosa_multiflora Rmu_sc0035020.1_g000006
rosa_roxburghii Rroxscaffold_7G00158490
rosa_rugosa Rorug06G0380700
rosa_samantha Rh6AG494700 Rh6BG504400 Rh6CG509600 Rh6DG495600
rosa_wichuraiana Rw6G043030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 384
AasI GACNNNNNNGTC 1 cut(s) 170
AatII GACGTC 1 cut(s) 96
Acc36I ACCTGC 1 cut(s) 1270
AccB1I GGYRCC 1 cut(s) 146
AccII CGCG 2 cut(s) 78, 181
AccIII TCCGGA 1 cut(s) 668
AciI CCGC 5 cut(s) 76, 130, 181, 1105, 1332
AclWI GGATC 1 cut(s) 1342
AcoI YGGCCR 4 cut(s) 12, 63, 1413, 1453
AcsI RAATTY 1 cut(s) 1151
AcuI CTGAAG 4 cut(s) 717, 1024, 1137, 1407
AcyI GRCGYC 2 cut(s) 93, 147
AdeI CACNNNGTG 1 cut(s) 183
AfaI GTAC 2 cut(s) 552, 849
AfeI AGCGCT 1 cut(s) 1252
AfiI CCNNNNNNNGG 3 cut(s) 140, 344, 1093
AflII CTTAAG 1 cut(s) 1298
AgsI TTSAA 6 cut(s) 265, 656, 706, 859, 1053, 1160
AjnI CCWGG 2 cut(s) 544, 1371
AjuI GAANNNNNNNTTGG 2 cut(s) 1137, 1169
AluBI AGCT 5 cut(s) 116, 659, 1058, 1136, 1464
AluI AGCT 5 cut(s) 116, 659, 1058, 1136, 1464
Alw21I GWGCWC 1 cut(s) 1466
Alw26I GTCTC 4 cut(s) 52, 84, 1173, 1316
AlwI GGATC 1 cut(s) 1342
Ama87I CYCGRG 1 cut(s) 763
Aor13HI TCCGGA 1 cut(s) 668
Aor51HI AGCGCT 1 cut(s) 1252
AoxI GGCC 6 cut(s) 12, 63, 186, 1043, 1413, 1453
ApeKI GCWGC 3 cut(s) 440, 620, 1270
ApoI RAATTY 1 cut(s) 1151
Asp700I GAANNNNTTC 1 cut(s) 1037
AspA2I CCTAGG 1 cut(s) 338
AspLEI GCGC 3 cut(s) 102, 149, 1253
AspS9I GGNCC 6 cut(s) 186, 500, 542, 1084, 1235, 1243
AsuC2I CCSGG 4 cut(s) 135, 472, 764, 765
AsuHPI GGTGA 5 cut(s) 383, 425, 821, 1116, 1321
AvaI CYCGRG 1 cut(s) 763
AvaII GGWCC 5 cut(s) 500, 542, 1084, 1235, 1243
AvrII CCTAGG 1 cut(s) 338
AxyI CCTNAGG 1 cut(s) 396
BalI TGGCCA 1 cut(s) 14
BanI GGYRCC 1 cut(s) 146
BanII GRGCYC 2 cut(s) 963, 1466
BbsI GAAGAC 1 cut(s) 680
Bbv12I GWGCWC 1 cut(s) 1466
BbvCI CCTCAGC 1 cut(s) 1247
BbvI GCAGC 3 cut(s) 427, 607, 1282
BccI CCATC 5 cut(s) 134, 826, 1054, 1405, 1452
BceAI ACGGC 4 cut(s) 11, 91, 173, 181
BciT130I CCWGG 2 cut(s) 546, 1373
BciVI GTATCC 2 cut(s) 206, 783
BclI TGATCA 1 cut(s) 508
BcnI CCSGG 4 cut(s) 135, 472, 764, 765
BcoDI GTCTC 4 cut(s) 52, 84, 1173, 1316
BfaI CTAG 3 cut(s) 44, 339, 777
BfmI CTRYAG 2 cut(s) 621, 720
BfoI RGCGCY 2 cut(s) 150, 1254
BfrI CTTAAG 1 cut(s) 1298
BfuAI ACCTGC 1 cut(s) 1270
BfuI GTATCC 2 cut(s) 206, 783
BisI GCNGC 4 cut(s) 76, 441, 621, 1271
BlnI CCTAGG 1 cut(s) 338
BlpI GCTNAGC 1 cut(s) 962
BlsI GCNGC 4 cut(s) 77, 442, 622, 1272
BmcAI AGTACT 1 cut(s) 552
Bme1390I CCNGG 6 cut(s) 135, 472, 546, 764, 765, 1373
Bme18I GGWCC 5 cut(s) 500, 542, 1084, 1235, 1243
BmeT110I CYCGRG 1 cut(s) 763
BmgT120I GGNCC 6 cut(s) 186, 500, 542, 1084, 1235, 1243
BmiI GGNNCC 4 cut(s) 148, 169, 205, 1113
BmrFI CCNGG 6 cut(s) 135, 472, 546, 764, 765, 1373
BmrI ACTGGG 2 cut(s) 417, 1297
BmsI GCATC 4 cut(s) 238, 549, 734, 1063
BmuI ACTGGG 2 cut(s) 417, 1297
BoxI GACNNNNGTC 1 cut(s) 498
BpiI GAAGAC 1 cut(s) 680
BpmI CTGGAG 1 cut(s) 567
Bpu10I CCTNAGC 1 cut(s) 1247
Bpu1102I GCTNAGC 1 cut(s) 962
BpuEI CTTGAG 1 cut(s) 1122
BpuMI CCSGG 4 cut(s) 135, 472, 764, 765
BsaBI GATNNNNATC 2 cut(s) 1077, 1339
BsaHI GRCGYC 2 cut(s) 93, 147
BsaJI CCNNGG 7 cut(s) 133, 338, 471, 763, 1127, 1371, 1416
BsaWI WCCGGW 1 cut(s) 668
BsaXI ACNNNNNCTCC 2 cut(s) 152, 182
Bsc4I CCNNNNNNNGG 3 cut(s) 140, 344, 1093
Bse118I RCCGGY 1 cut(s) 200
Bse1I ACTGG 6 cut(s) 69, 221, 412, 567, 894, 1292
Bse21I CCTNAGG 1 cut(s) 396
Bse3DI GCAATG 1 cut(s) 1170
Bse8I GATNNNNATC 2 cut(s) 1077, 1339
BseAI TCCGGA 1 cut(s) 668
BseBI CCWGG 2 cut(s) 546, 1373
BseDI CCNNGG 7 cut(s) 133, 338, 471, 763, 1127, 1371, 1416
BseGI GGATG 4 cut(s) 564, 837, 1078, 1359
BseJI GATNNNNATC 2 cut(s) 1077, 1339
BseLI CCNNNNNNNGG 3 cut(s) 140, 344, 1093
BseMI GCAATG 1 cut(s) 1170
BseMII CTCAG 4 cut(s) 976, 1043, 1261, 1358
BseNI ACTGG 6 cut(s) 69, 221, 412, 567, 894, 1292
BseRI GAGGAG 1 cut(s) 1327
BseXI GCAGC 3 cut(s) 427, 607, 1282
BseYI CCCAGC 1 cut(s) 1265
BsgI GTGCAG 1 cut(s) 1454
Bsh1236I CGCG 2 cut(s) 78, 181
BshFI GGCC 6 cut(s) 14, 65, 188, 1045, 1415, 1455
BshNI GGYRCC 1 cut(s) 146
BsiHKAI GWGCWC 1 cut(s) 1466
BsiHKCI CYCGRG 1 cut(s) 763
BsiSI CCGG 5 cut(s) 135, 201, 471, 669, 764
BslFI GGGAC 5 cut(s) 555, 640, 766, 1006, 1304
BslI CCNNNNNNNGG 3 cut(s) 140, 344, 1093
BsmAI GTCTC 4 cut(s) 52, 84, 1173, 1316
BsmBI CGTCTC 2 cut(s) 84, 1316
BsmFI GGGAC 5 cut(s) 555, 640, 766, 1006, 1304
BsmI GAATGC 1 cut(s) 1182
BsnI GGCC 6 cut(s) 14, 65, 188, 1045, 1415, 1455
BsoBI CYCGRG 1 cut(s) 763
Bsp1286I GDGCHC 2 cut(s) 963, 1466
Bsp13I TCCGGA 1 cut(s) 668
Bsp143I GATC 7 cut(s) 84, 151, 195, 508, 532, 1078, 1334
Bsp1720I GCTNAGC 1 cut(s) 962
BspACI CCGC 5 cut(s) 76, 130, 181, 1105, 1332
BspANI GGCC 6 cut(s) 14, 65, 188, 1045, 1415, 1455
BspCNI CTCAG 4 cut(s) 975, 1042, 1260, 1357
BspEI TCCGGA 1 cut(s) 668
BspFNI CGCG 2 cut(s) 78, 181
BspLI GGNNCC 4 cut(s) 148, 169, 205, 1113
BspMAI CTGCAG 1 cut(s) 625
BspMI ACCTGC 1 cut(s) 1270
BspPI GGATC 1 cut(s) 1342
BspT107I GGYRCC 1 cut(s) 146
BspTI CTTAAG 1 cut(s) 1298
BsrDI GCAATG 1 cut(s) 1170
BsrFI RCCGGY 1 cut(s) 200
BsrI ACTGG 6 cut(s) 69, 221, 412, 567, 894, 1292
BssAI RCCGGY 1 cut(s) 200
BssECI CCNNGG 7 cut(s) 133, 338, 471, 763, 1127, 1371, 1416
BssMI GATC 7 cut(s) 84, 151, 195, 508, 532, 1078, 1334
BssNI GRCGYC 2 cut(s) 93, 147
BssT1I CCWWGG 2 cut(s) 338, 1127
Bst2UI CCWGG 2 cut(s) 546, 1373
Bst4CI ACNGT 6 cut(s) 19, 362, 371, 424, 497, 1295
Bst6I CTCTTC 4 cut(s) 61, 324, 732, 983
BstACI GRCGYC 2 cut(s) 93, 147
BstAFI CTTAAG 1 cut(s) 1298
BstAPI GCANNNNNTGC 1 cut(s) 731
BstC8I GCNNGC 5 cut(s) 104, 179, 202, 618, 1435
BstDEI CTNAG 5 cut(s) 396, 962, 1029, 1247, 1344
BstENI CCTNNNNNAGG 1 cut(s) 342
BstF5I GGATG 4 cut(s) 564, 837, 1078, 1359
BstFNI CGCG 2 cut(s) 78, 181
BstH2I RGCGCY 2 cut(s) 150, 1254
BstHHI GCGC 3 cut(s) 102, 149, 1253
BstKTI GATC 7 cut(s) 87, 154, 198, 511, 535, 1081, 1337
BstMAI GTCTC 4 cut(s) 52, 84, 1173, 1316
BstMBI GATC 7 cut(s) 84, 151, 195, 508, 532, 1078, 1334
BstMWI GCNNNNNNNGC 5 cut(s) 144, 174, 722, 731, 1461
BstNI CCWGG 2 cut(s) 546, 1373
BstNSI RCATGY 2 cut(s) 620, 1366
BstPAI GACNNNNGTC 1 cut(s) 498
BstSCI CCNGG 6 cut(s) 133, 470, 544, 762, 763, 1371
BstSFI CTRYAG 2 cut(s) 621, 720
BstUI CGCG 2 cut(s) 78, 181
BstV1I GCAGC 3 cut(s) 427, 607, 1282
BstV2I GAAGAC 1 cut(s) 680
BstX2I RGATCY 1 cut(s) 1334
BstYI RGATCY 1 cut(s) 1334
Bsu36I CCTNAGG 1 cut(s) 396
BsuI GTATCC 2 cut(s) 206, 783
BsuRI GGCC 6 cut(s) 14, 65, 188, 1045, 1415, 1455
BtgZI GCGATG 1 cut(s) 153
BtsCI GGATG 4 cut(s) 564, 837, 1078, 1359
BtsIMutI CAGTG 4 cut(s) 15, 405, 502, 1489
BveI ACCTGC 1 cut(s) 1270
Cac8I GCNNGC 5 cut(s) 104, 179, 202, 618, 1435
CfoI GCGC 3 cut(s) 102, 149, 1253
Cfr10I RCCGGY 1 cut(s) 200
Cfr13I GGNCC 6 cut(s) 186, 500, 542, 1084, 1235, 1243
Cfr9I CCCGGG 1 cut(s) 763
CseI GACGC 1 cut(s) 1426
Csp6I GTAC 2 cut(s) 551, 848
CviAII CATG 9 cut(s) 248, 536, 617, 935, 940, 982, 1024, 1195, 1363
CviQI GTAC 2 cut(s) 551, 848
DdeI CTNAG 5 cut(s) 396, 962, 1029, 1247, 1344
DinI GGCGCC 1 cut(s) 148
DpnI GATC 7 cut(s) 86, 153, 197, 510, 534, 1080, 1336
DpnII GATC 7 cut(s) 84, 151, 195, 508, 532, 1078, 1334
DraIII CACNNNGTG 1 cut(s) 183
DrdI GACNNNNNNGTC 1 cut(s) 170
DseDI GACNNNNNNGTC 1 cut(s) 170
EaeI YGGCCR 4 cut(s) 12, 63, 1413, 1453
Eam1104I CTCTTC 4 cut(s) 61, 324, 732, 983
EarI CTCTTC 4 cut(s) 61, 324, 732, 983
EciI GGCGGA 1 cut(s) 119
Ecl136II GAGCTC 1 cut(s) 1464
Eco130I CCWWGG 2 cut(s) 338, 1127
Eco24I GRGCYC 2 cut(s) 963, 1466
Eco47I GGWCC 5 cut(s) 500, 542, 1084, 1235, 1243
Eco47III AGCGCT 1 cut(s) 1252
Eco53kI GAGCTC 1 cut(s) 1464
Eco57I CTGAAG 4 cut(s) 717, 1024, 1137, 1407
Eco81I CCTNAGG 1 cut(s) 396
Eco88I CYCGRG 1 cut(s) 763
EcoICRI GAGCTC 1 cut(s) 1464
EcoNI CCTNNNNNAGG 1 cut(s) 342
EcoO109I RGGNCCY 1 cut(s) 1243
EcoRII CCWGG 2 cut(s) 544, 1371
EcoT14I CCWWGG 2 cut(s) 338, 1127
EcoT22I ATGCAT 1 cut(s) 1182
EcoT38I GRGCYC 2 cut(s) 963, 1466
EgeI GGCGCC 1 cut(s) 148
EheI GGCGCC 1 cut(s) 148
ErhI CCWWGG 2 cut(s) 338, 1127
Esp3I CGTCTC 2 cut(s) 84, 1316
FaeI CATG 9 cut(s) 251, 539, 620, 938, 943, 985, 1027, 1198, 1366
FalI AAGNNNNNCTT 2 cut(s) 1140, 1172
FaqI GGGAC 5 cut(s) 555, 640, 766, 1006, 1304
FatI CATG 9 cut(s) 247, 535, 616, 934, 939, 981, 1023, 1194, 1362
FauNDI CATATG 1 cut(s) 631
FbaI TGATCA 1 cut(s) 508
Fnu4HI GCNGC 4 cut(s) 76, 441, 621, 1271
FokI GGATG 4 cut(s) 571, 844, 1085, 1366
FriOI GRGCYC 2 cut(s) 963, 1466
Fsp4HI GCNGC 4 cut(s) 76, 441, 621, 1271
FspBI CTAG 3 cut(s) 44, 339, 777
GlaI GCGC 3 cut(s) 101, 148, 1252
GluI GCNGC 4 cut(s) 76, 441, 621, 1271
GsaI CCCAGC 1 cut(s) 1269
GsuI CTGGAG 1 cut(s) 567
HaeII RGCGCY 2 cut(s) 150, 1254
HaeIII GGCC 6 cut(s) 14, 65, 188, 1045, 1415, 1455
HapII CCGG 5 cut(s) 135, 201, 471, 669, 764
HgaI GACGC 1 cut(s) 1426
HhaI GCGC 3 cut(s) 102, 149, 1253
Hin1I GRCGYC 2 cut(s) 93, 147
Hin1II CATG 9 cut(s) 251, 539, 620, 938, 943, 985, 1027, 1198, 1366
Hin6I GCGC 3 cut(s) 100, 147, 1251
HinP1I GCGC 3 cut(s) 100, 147, 1251
HindIII AAGCTT 2 cut(s) 114, 657
HinfI GANTC 5 cut(s) 4, 348, 665, 1224, 1479
HpaII CCGG 5 cut(s) 135, 201, 471, 669, 764
HphI GGTGA 5 cut(s) 383, 425, 821, 1116, 1321
Hpy166II GTNNAC 3 cut(s) 493, 850, 1063
Hpy188I TCNGA 6 cut(s) 89, 112, 504, 532, 865, 1032
Hpy188III TCNNGA 7 cut(s) 158, 653, 669, 695, 1115, 1157, 1238
Hpy8I GTNNAC 3 cut(s) 493, 850, 1063
Hpy99I CGWCG 3 cut(s) 26, 167, 176
HpyAV CCTTC 6 cut(s) 41, 262, 692, 1112, 1396, 1458
HpyCH4III ACNGT 6 cut(s) 19, 362, 371, 424, 497, 1295
HpyCH4IV ACGT 3 cut(s) 93, 846, 1325
HpyF10VI GCNNNNNNNGC 5 cut(s) 144, 174, 722, 731, 1461
HpyF3I CTNAG 5 cut(s) 396, 962, 1029, 1247, 1344
HpySE526I ACGT 3 cut(s) 93, 846, 1325
Hsp92I GRCGYC 2 cut(s) 93, 147
Hsp92II CATG 9 cut(s) 251, 539, 620, 938, 943, 985, 1027, 1198, 1366
HspAI GCGC 3 cut(s) 100, 147, 1251
KasI GGCGCC 1 cut(s) 146
Kpn2I TCCGGA 1 cut(s) 668
KroI GCCGGC 1 cut(s) 200
KroNI GCCGGC 1 cut(s) 202
Ksp22I TGATCA 1 cut(s) 508
Kzo9I GATC 7 cut(s) 84, 151, 195, 508, 532, 1078, 1334
LmnI GCTCC 5 cut(s) 173, 209, 1117, 1405, 1461
Lsp1109I GCAGC 3 cut(s) 427, 607, 1282
LweI GCATC 4 cut(s) 238, 549, 734, 1063
MaeI CTAG 3 cut(s) 44, 339, 777
MaeII ACGT 3 cut(s) 93, 846, 1325
MaeIII GTNAC 3 cut(s) 418, 809, 842
MalI GATC 7 cut(s) 86, 153, 197, 510, 534, 1080, 1336
MboI GATC 7 cut(s) 84, 151, 195, 508, 532, 1078, 1334
MflI RGATCY 1 cut(s) 1334
MhlI GDGCHC 2 cut(s) 963, 1466
MlsI TGGCCA 1 cut(s) 14
MluCI AATT 5 cut(s) 220, 257, 430, 916, 1151
MluNI TGGCCA 1 cut(s) 14
Mly113I GGCGCC 1 cut(s) 147
MlyI GAGTC 1 cut(s) 357
Mox20I TGGCCA 1 cut(s) 14
Mph1103I ATGCAT 1 cut(s) 1182
MroI TCCGGA 1 cut(s) 668
MroNI GCCGGC 1 cut(s) 200
MroXI GAANNNNTTC 1 cut(s) 1037
MscI TGGCCA 1 cut(s) 14
MseI TTAA 2 cut(s) 426, 1299
MslI CAYNNNNRTG 4 cut(s) 372, 986, 1022, 1185
Msp20I TGGCCA 1 cut(s) 14
MspCI CTTAAG 1 cut(s) 1298
MspI CCGG 5 cut(s) 135, 201, 471, 669, 764
MspR9I CCNGG 6 cut(s) 135, 472, 546, 764, 765, 1373
Mva1269I GAATGC 1 cut(s) 1182
MvaI CCWGG 2 cut(s) 546, 1373
MvnI CGCG 2 cut(s) 78, 181
MwoI GCNNNNNNNGC 5 cut(s) 144, 174, 722, 731, 1461
NaeI GCCGGC 1 cut(s) 202
NarI GGCGCC 1 cut(s) 147
NciI CCSGG 4 cut(s) 135, 472, 764, 765
NdeI CATATG 1 cut(s) 631
NdeII GATC 7 cut(s) 84, 151, 195, 508, 532, 1078, 1334
NgoMIV GCCGGC 1 cut(s) 200
NlaIII CATG 9 cut(s) 251, 539, 620, 938, 943, 985, 1027, 1198, 1366
NlaIV GGNNCC 4 cut(s) 148, 169, 205, 1113
NmeAIII GCCGAG 1 cut(s) 1441
NmuCI GTSAC 2 cut(s) 809, 842
NsiI ATGCAT 1 cut(s) 1182
NspI RCATGY 2 cut(s) 620, 1366
PaeI GCATGC 1 cut(s) 620
PcsI WCGNNNNNNNCGW 1 cut(s) 168
PctI GAATGC 1 cut(s) 1182
PdiI GCCGGC 1 cut(s) 202
PdmI GAANNNNTTC 1 cut(s) 1037
PfeI GAWTC 3 cut(s) 665, 1224, 1479
PfoI TCCNGGA 1 cut(s) 544
PkrI GCNGC 4 cut(s) 77, 442, 622, 1272
PleI GAGTC 1 cut(s) 356
PluTI GGCGCC 1 cut(s) 150
PpsI GAGTC 1 cut(s) 356
PpuMI RGGWCCY 1 cut(s) 1243
PshAI GACNNNNGTC 1 cut(s) 498
PsiI TTATAA 1 cut(s) 384
Psp124BI GAGCTC 1 cut(s) 1466
Psp5II RGGWCCY 1 cut(s) 1243
Psp6I CCWGG 2 cut(s) 544, 1371
PspFI CCCAGC 1 cut(s) 1265
PspGI CCWGG 2 cut(s) 544, 1371
PspN4I GGNNCC 4 cut(s) 148, 169, 205, 1113
PspPI GGNCC 6 cut(s) 186, 500, 542, 1084, 1235, 1243
PspPPI RGGWCCY 1 cut(s) 1243
PstI CTGCAG 1 cut(s) 625
PsuI RGATCY 1 cut(s) 1334
RsaI GTAC 2 cut(s) 552, 849
RsaNI GTAC 2 cut(s) 551, 848
RseI CAYNNNNRTG 4 cut(s) 372, 986, 1022, 1185
SacI GAGCTC 1 cut(s) 1466
SaqAI TTAA 2 cut(s) 426, 1299
SatI GCNGC 4 cut(s) 76, 441, 621, 1271
Sau3AI GATC 7 cut(s) 84, 151, 195, 508, 532, 1078, 1334
Sau96I GGNCC 6 cut(s) 186, 500, 542, 1084, 1235, 1243
ScaI AGTACT 1 cut(s) 552
SchI GAGTC 1 cut(s) 357
ScrFI CCNGG 6 cut(s) 135, 472, 546, 764, 765, 1373
SduI GDGCHC 2 cut(s) 963, 1466
SfaNI GCATC 4 cut(s) 238, 549, 734, 1063
SfcI CTRYAG 2 cut(s) 621, 720
SfoI GGCGCC 1 cut(s) 148
SinI GGWCC 5 cut(s) 500, 542, 1084, 1235, 1243
SmaI CCCGGG 1 cut(s) 765
SmiMI CAYNNNNRTG 4 cut(s) 372, 986, 1022, 1185
SmlI CTYRAG 2 cut(s) 1137, 1298
SmoI CTYRAG 2 cut(s) 1137, 1298
SphI GCATGC 1 cut(s) 620
Sse9I AATT 5 cut(s) 220, 257, 430, 916, 1151
SsiI CCGC 5 cut(s) 76, 130, 181, 1105, 1332
SspDI GGCGCC 1 cut(s) 146
SspI AATATT 1 cut(s) 292
SspMI CTAG 3 cut(s) 44, 339, 777
SstI GAGCTC 1 cut(s) 1466
StyD4I CCNGG 6 cut(s) 133, 470, 544, 762, 763, 1371
StyI CCWWGG 2 cut(s) 338, 1127
TaaI ACNGT 6 cut(s) 19, 362, 371, 424, 497, 1295
TaiI ACGT 3 cut(s) 96, 849, 1328
TaqI TCGA 2 cut(s) 119, 1258
TasI AATT 5 cut(s) 220, 257, 430, 916, 1151
TatI WGTACW 1 cut(s) 550
TauI GCSGC 1 cut(s) 78
TfiI GAWTC 3 cut(s) 665, 1224, 1479
Tru1I TTAA 2 cut(s) 426, 1299
Tru9I TTAA 2 cut(s) 426, 1299
TscAI CASTG 4 cut(s) 22, 412, 502, 1489
TseFI GTSAC 2 cut(s) 809, 842
TseI GCWGC 3 cut(s) 440, 620, 1270
Tsp45I GTSAC 2 cut(s) 809, 842
TspGWI ACGGA 1 cut(s) 160
TspMI CCCGGG 1 cut(s) 763
TspRI CASTG 4 cut(s) 22, 412, 502, 1489
Vha464I CTTAAG 1 cut(s) 1298
VpaK11BI GGWCC 5 cut(s) 500, 542, 1084, 1235, 1243
XagI CCTNNNNNAGG 1 cut(s) 342
XapI RAATTY 1 cut(s) 1151
XceI RCATGY 2 cut(s) 620, 1366
XmaI CCCGGG 1 cut(s) 763
XmaJI CCTAGG 1 cut(s) 338
XmnI GAANNNNTTC 1 cut(s) 1037
XspI CTAG 3 cut(s) 44, 339, 777
ZraI GACGTC 1 cut(s) 94
ZrmI AGTACT 1 cut(s) 552
Zsp2I ATGCAT 1 cut(s) 1182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.