MD08G1073500.v1.1

Vacuolar-processing enzyme-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0009785
Physical Location & Seq
Forward (+)
5935066 .. 5939080
4015 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1073500.v1.1.491

Sequence Viewer

Length: 1485 bp
ATGACTCGGTTGGCCAGCGCCCTCGTCCTCCTGTTCCTCGCCTCCGTCTCGGCTTCTGCAGCCGGTAGCCGGGACCTTAACGGAAACGTTTTGCGATTACCTTCGGAAGCTTCCAGATTCTTTGGGAGAGGCGATAACGCTCCCGATCAGGACGACGGCACCGTCGGGACCAGATGGGCTGTTCTGATCGCCGGCTCCAATGGATACTGGAATTACAGACATCAGGCTGATATTTGTCATGCCTATCAACTACTGAAGAAAGGAGGGTTGAAAGATGAAAACATCGTTGTATTCATGTACGATGATATTGCTTACAATGAGGAGAACCCTAGGCAAGGAGTCATCATTAACAGCCCGCATGGGAGTGACGTTTACAAAGGAGTTCCAAAGGATTACACTGGCCAAGATGTTACTGTGAACAACTTTTTTGCTGCTATCCTTGGGAACAAATCTGCTCTTACAGGGGGTAGTGGGAAGGTTGTGGATAGCGGTCCAAATGATCATATTTTCATATACTACACCGATCATGGTGGTCCTGGAATTCTTGGGATGCCGGCAACCAGTCCTTATATTTATGCCAACGATCTGATAGAAGTCCTGAAGAAGAAGCATGCTGCAGGAACCTATAAAAGCATGGTATTTTATCTCGAAGCTTGTGAATCTGGAAGCATCTTCGAGGGCCTTCTTCCTGAAGGTTTGAATATCTTTGCAACCACTGCATCAAATGCAGAAGAGAGCAGTTGGGGGACGTACTGCCCTGGAGAGTATCCCAGTCCTCCTCCAGAATATGAAACCTGCTTGGGTGACTTGTATAGTGTTGCGTGGATGGAAGACAGTGACATACACAACTTGAAGTCAGAAACCTTGCACCAGCAGTATGAACTGGTTAAAACAAGGACAGCAAATGACAATTCTGGCTTTGGTTCTCATGTCATGCAATACGGCGATGTAGGGCTCAGCAAGAACAACCTCTTTGTGTACATGGGGACAAATCCTGCAAACGAGAACTTTACTTTTCTGGGCCAGAACTCCTTGAGGCCATCTTCAAAAGCTGTCAACCAGCGCGATGCTGATCTTGTTCATTTCTGGCATAAGTATAGCAAGGCTCCCGAAGGATCACCAAGGAAAGTTCAAGCTCAAAAGGAATTTGTTGAAGCAATGTCTCATAGAATGCACATAGATGAAACCATGAAGCTCATTGGGAAACTCCTGTTTGGAATTGAAAAAGGTCCAAAAGTTCTCAACGCTGTTCGACCAGCTGGCCAGCCGCTCGTTGATGACTGGGACTGCCTTAAGACAATGGTGAGGAGTTTTGAGACACATTGTGGATCCCTATCTCAATACGGAATGAAACACATGCGTTCCCTGGCAAACATCTGCAACGCCGGCATGACAAAGGAGCAGATGGCGGAGGCTTCAGCACAAGCCTGCGTCAGCGCCCCTTCTGATCGCTGGAGCTCCCTGCACATGGGATTCAGTGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04612 map04142 map04612
Pfam Domains
Protein Families
Peptidase_C13

Protein Analysis

495

Amino Acids

54.16

Weight (kDa)

5.81

Isoelectric Point (pI)

43.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C13 PF01650 58 - 330 1.2e-118 Peptidase C13 family
Legum_prodom PF20985 382 - 477 1.1e-43 Legumain, prodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009785)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G25940 AT4G32940
fragaria_vesca FvH4_2g32190 FvH4_2g32190 FvH4_2g32190 FvH4_2g32190
malus_domestica MD08G1073500.v1.1
prunus_persica Prupe.1G415000_v2.0.a1 Prupe.1G415000_v2.0.a1
pyrus_communis pycom08g06090 pycom15g05790
rosa_chinensis RchiOBHm_Chr6g0310481
rosa_laevigata RLG00000010479
rosa_multiflora Rmu_sc0035020.1_g000006
rosa_roxburghii Rroxscaffold_7G00158490
rosa_rugosa Rorug06G0380700
rosa_samantha Rh6AG494700 Rh6BG504400 Rh6CG509600 Rh6DG495600
rosa_wichuraiana Rw6G043030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 161
Acc36I ACCTGC 1 cut(s) 803
AccB1I GGYRCC 1 cut(s) 158
AccBSI CCGCTC 1 cut(s) 1270
AccII CGCG 1 cut(s) 1065
AciI CCGC 4 cut(s) 356, 489, 1268, 1409
AclI AACGTT 1 cut(s) 87
AclWI GGATC 3 cut(s) 1123, 1323, 1336
AcoI YGGCCR 3 cut(s) 12, 400, 1261
AcsI RAATTY 2 cut(s) 540, 1145
AcuI CTGAAG 4 cut(s) 275, 620, 711, 1401
AdeI CACNNNGTG 1 cut(s) 1325
AfaI GTAC 3 cut(s) 299, 752, 980
AfiI CCNNNNNNNGG 3 cut(s) 69, 335, 1468
AflII CTTAAG 1 cut(s) 1292
AgsI TTSAA 7 cut(s) 271, 700, 853, 1047, 1133, 1154, 1223
AjnI CCWGG 3 cut(s) 535, 757, 1365
AluBI AGCT 7 cut(s) 110, 653, 1052, 1136, 1195, 1259, 1458
AluI AGCT 7 cut(s) 110, 653, 1052, 1136, 1195, 1259, 1458
Alw21I GWGCWC 1 cut(s) 1460
Alw26I GTCTC 3 cut(s) 52, 1167, 1310
AlwI GGATC 3 cut(s) 1123, 1323, 1336
AoxI GGCC 6 cut(s) 12, 400, 679, 1021, 1037, 1261
ApeKI GCWGC 3 cut(s) 59, 431, 614
ApoI RAATTY 2 cut(s) 540, 1145
AspA2I CCTAGG 1 cut(s) 329
AspLEI GCGC 3 cut(s) 20, 1065, 1439
AspS9I GGNCC 7 cut(s) 73, 168, 491, 533, 679, 1021, 1229
AsuC2I CCSGG 1 cut(s) 71
AsuHPI GGTGA 3 cut(s) 815, 1110, 1315
AvaII GGWCC 5 cut(s) 73, 168, 491, 533, 1229
AvrII CCTAGG 1 cut(s) 329
BalI TGGCCA 3 cut(s) 14, 402, 1263
BamHI GGATCC 1 cut(s) 1328
BanI GGYRCC 1 cut(s) 158
BanII GRGCYC 2 cut(s) 957, 1460
BbsI GAAGAC 1 cut(s) 837
Bbv12I GWGCWC 1 cut(s) 1460
BbvI GCAGC 3 cut(s) 71, 418, 601
BccI CCATC 4 cut(s) 168, 820, 1048, 1399
BceAI ACGGC 2 cut(s) 172, 958
BcgI CGANNNNNNTGC 2 cut(s) 290, 324
BciT130I CCWGG 3 cut(s) 537, 759, 1367
BciVI GTATCC 2 cut(s) 197, 777
BclI TGATCA 1 cut(s) 499
BcnI CCSGG 1 cut(s) 71
BcoDI GTCTC 3 cut(s) 52, 1167, 1310
BfaI CTAG 1 cut(s) 330
BfmI CTRYAG 2 cut(s) 57, 615
BfoI RGCGCY 2 cut(s) 21, 1440
BfrI CTTAAG 1 cut(s) 1292
BfuAI ACCTGC 1 cut(s) 803
BfuI GTATCC 2 cut(s) 197, 777
BisI GCNGC 4 cut(s) 60, 432, 615, 1268
BlnI CCTAGG 1 cut(s) 329
BlpI GCTNAGC 1 cut(s) 956
BlsI GCNGC 4 cut(s) 61, 433, 616, 1269
Bme1390I CCNGG 4 cut(s) 71, 537, 759, 1367
Bme18I GGWCC 5 cut(s) 73, 168, 491, 533, 1229
BmgT120I GGNCC 7 cut(s) 73, 168, 491, 533, 679, 1021, 1229
BmiI GGNNCC 7 cut(s) 74, 160, 169, 196, 622, 1107, 1330
BmrFI CCNGG 4 cut(s) 71, 537, 759, 1367
BmrI ACTGGG 2 cut(s) 765, 1291
BmsI GCATC 4 cut(s) 540, 678, 728, 1057
BmuI ACTGGG 2 cut(s) 765, 1291
BpiI GAAGAC 1 cut(s) 837
BpmI CTGGAG 3 cut(s) 765, 780, 1474
Bpu1102I GCTNAGC 1 cut(s) 956
BpuEI CTTGAG 1 cut(s) 1054
BpuMI CCSGG 1 cut(s) 71
BsaBI GATNNNNATC 2 cut(s) 1071, 1333
BsaJI CCNNGG 5 cut(s) 329, 439, 757, 1121, 1365
Bsc4I CCNNNNNNNGG 3 cut(s) 69, 335, 1468
Bse118I RCCGGY 4 cut(s) 62, 191, 553, 1385
Bse1I ACTGG 6 cut(s) 212, 403, 561, 771, 888, 1286
Bse3DI GCAATG 1 cut(s) 1164
Bse8I GATNNNNATC 2 cut(s) 1071, 1333
BseBI CCWGG 3 cut(s) 537, 759, 1367
BseDI CCNNGG 5 cut(s) 329, 439, 757, 1121, 1365
BseGI GGATG 2 cut(s) 555, 831
BseJI GATNNNNATC 2 cut(s) 1071, 1333
BseLI CCNNNNNNNGG 3 cut(s) 69, 335, 1468
BseMI GCAATG 1 cut(s) 1164
BseMII CTCAG 1 cut(s) 970
BseNI ACTGG 6 cut(s) 212, 403, 561, 771, 888, 1286
BseRI GAGGAG 3 cut(s) 335, 768, 1321
BseXI GCAGC 3 cut(s) 71, 418, 601
BsgI GTGCAG 1 cut(s) 1448
Bsh1236I CGCG 1 cut(s) 1065
BshFI GGCC 6 cut(s) 14, 402, 681, 1023, 1039, 1263
BshNI GGYRCC 1 cut(s) 158
BsiHKAI GWGCWC 1 cut(s) 1460
BsiSI CCGG 5 cut(s) 63, 70, 192, 554, 1386
BslFI GGGAC 5 cut(s) 86, 181, 760, 1000, 1298
BslI CCNNNNNNNGG 3 cut(s) 69, 335, 1468
BsmAI GTCTC 3 cut(s) 52, 1167, 1310
BsmBI CGTCTC 1 cut(s) 52
BsmFI GGGAC 5 cut(s) 86, 181, 760, 1000, 1298
BsmI GAATGC 1 cut(s) 1176
BsnI GGCC 6 cut(s) 14, 402, 681, 1023, 1039, 1263
Bsp1286I GDGCHC 2 cut(s) 957, 1460
Bsp1407I TGTACA 1 cut(s) 978
Bsp143I GATC 9 cut(s) 145, 186, 499, 523, 583, 1072, 1115, 1328, 1447
Bsp1720I GCTNAGC 1 cut(s) 956
BspACI CCGC 4 cut(s) 356, 489, 1268, 1409
BspANI GGCC 6 cut(s) 14, 402, 681, 1023, 1039, 1263
BspCNI CTCAG 1 cut(s) 969
BspFNI CGCG 1 cut(s) 1065
BspLI GGNNCC 7 cut(s) 74, 160, 169, 196, 622, 1107, 1330
BspMAI CTGCAG 2 cut(s) 61, 619
BspMI ACCTGC 1 cut(s) 803
BspPI GGATC 3 cut(s) 1123, 1323, 1336
BspT107I GGYRCC 1 cut(s) 158
BspTI CTTAAG 1 cut(s) 1292
BsrBI CCGCTC 1 cut(s) 1270
BsrDI GCAATG 1 cut(s) 1164
BsrFI RCCGGY 4 cut(s) 62, 191, 553, 1385
BsrGI TGTACA 1 cut(s) 978
BsrI ACTGG 6 cut(s) 212, 403, 561, 771, 888, 1286
BssAI RCCGGY 4 cut(s) 62, 191, 553, 1385
BssECI CCNNGG 5 cut(s) 329, 439, 757, 1121, 1365
BssMI GATC 9 cut(s) 145, 186, 499, 523, 583, 1072, 1115, 1328, 1447
BssT1I CCWWGG 3 cut(s) 329, 439, 1121
Bst2UI CCWGG 3 cut(s) 537, 759, 1367
Bst4CI ACNGT 3 cut(s) 163, 415, 836
Bst6I CTCTTC 1 cut(s) 726
BstAFI CTTAAG 1 cut(s) 1292
BstAPI GCANNNNNTGC 2 cut(s) 716, 725
BstAUI TGTACA 1 cut(s) 978
BstC8I GCNNGC 9 cut(s) 16, 193, 356, 555, 612, 1261, 1265, 1387, 1429
BstDEI CTNAG 1 cut(s) 956
BstENI CCTNNNNNAGG 1 cut(s) 333
BstF5I GGATG 2 cut(s) 555, 831
BstFNI CGCG 1 cut(s) 1065
BstH2I RGCGCY 2 cut(s) 21, 1440
BstHHI GCGC 3 cut(s) 20, 1065, 1439
BstKTI GATC 9 cut(s) 148, 189, 502, 526, 586, 1075, 1118, 1331, 1450
BstMAI GTCTC 3 cut(s) 52, 1167, 1310
BstMBI GATC 9 cut(s) 145, 186, 499, 523, 583, 1072, 1115, 1328, 1447
BstMWI GCNNNNNNNGC 4 cut(s) 59, 716, 725, 1386
BstNI CCWGG 3 cut(s) 537, 759, 1367
BstNSI RCATGY 2 cut(s) 614, 1360
BstSCI CCNGG 4 cut(s) 69, 535, 757, 1365
BstSFI CTRYAG 2 cut(s) 57, 615
BstUI CGCG 1 cut(s) 1065
BstV1I GCAGC 3 cut(s) 71, 418, 601
BstV2I GAAGAC 1 cut(s) 837
BstX2I RGATCY 1 cut(s) 1328
BstYI RGATCY 1 cut(s) 1328
BsuI GTATCC 2 cut(s) 197, 777
BsuRI GGCC 6 cut(s) 14, 402, 681, 1023, 1039, 1263
BtgZI GCGATG 2 cut(s) 960, 1080
BtsCI GGATG 2 cut(s) 555, 831
BtsI GCAGTG 1 cut(s) 714
BtsIMutI CAGTG 4 cut(s) 396, 714, 841, 1483
BveI ACCTGC 1 cut(s) 803
Cac8I GCNNGC 9 cut(s) 16, 193, 356, 555, 612, 1261, 1265, 1387, 1429
CfoI GCGC 3 cut(s) 20, 1065, 1439
Cfr10I RCCGGY 4 cut(s) 62, 191, 553, 1385
Cfr13I GGNCC 7 cut(s) 73, 168, 491, 533, 679, 1021, 1229
CseI GACGC 1 cut(s) 1420
Csp6I GTAC 3 cut(s) 298, 751, 979
CviQI GTAC 3 cut(s) 298, 751, 979
DdeI CTNAG 1 cut(s) 956
DpnI GATC 9 cut(s) 147, 188, 501, 525, 585, 1074, 1117, 1330, 1449
DpnII GATC 9 cut(s) 145, 186, 499, 523, 583, 1072, 1115, 1328, 1447
DraIII CACNNNGTG 1 cut(s) 1325
DrdI GACNNNNNNGTC 1 cut(s) 161
DseDI GACNNNNNNGTC 1 cut(s) 161
EaeI YGGCCR 3 cut(s) 12, 400, 1261
Eam1104I CTCTTC 1 cut(s) 726
EarI CTCTTC 1 cut(s) 726
EciI GGCGGA 1 cut(s) 1424
Ecl136II GAGCTC 1 cut(s) 1458
Eco130I CCWWGG 3 cut(s) 329, 439, 1121
Eco24I GRGCYC 2 cut(s) 957, 1460
Eco47I GGWCC 5 cut(s) 73, 168, 491, 533, 1229
Eco53kI GAGCTC 1 cut(s) 1458
Eco57I CTGAAG 4 cut(s) 275, 620, 711, 1401
EcoICRI GAGCTC 1 cut(s) 1458
EcoNI CCTNNNNNAGG 1 cut(s) 333
EcoO109I RGGNCCY 2 cut(s) 73, 679
EcoRI GAATTC 1 cut(s) 540
EcoRII CCWGG 3 cut(s) 535, 757, 1365
EcoT14I CCWWGG 3 cut(s) 329, 439, 1121
EcoT38I GRGCYC 2 cut(s) 957, 1460
ErhI CCWWGG 3 cut(s) 329, 439, 1121
Esp3I CGTCTC 1 cut(s) 52
FaqI GGGAC 5 cut(s) 86, 181, 760, 1000, 1298
FauI CCCGC 1 cut(s) 363
FbaI TGATCA 1 cut(s) 499
Fnu4HI GCNGC 4 cut(s) 60, 432, 615, 1268
FokI GGATG 2 cut(s) 562, 838
FriOI GRGCYC 2 cut(s) 957, 1460
Fsp4HI GCNGC 4 cut(s) 60, 432, 615, 1268
FspBI CTAG 1 cut(s) 330
GlaI GCGC 3 cut(s) 19, 1064, 1438
GluI GCNGC 4 cut(s) 60, 432, 615, 1268
GsuI CTGGAG 3 cut(s) 765, 780, 1474
HaeII RGCGCY 2 cut(s) 21, 1440
HaeIII GGCC 6 cut(s) 14, 402, 681, 1023, 1039, 1263
HapII CCGG 5 cut(s) 63, 70, 192, 554, 1386
HgaI GACGC 1 cut(s) 1420
HhaI GCGC 3 cut(s) 20, 1065, 1439
Hin6I GCGC 3 cut(s) 18, 1063, 1437
HinP1I GCGC 3 cut(s) 18, 1063, 1437
HincII GTYRAC 1 cut(s) 1057
HindII GTYRAC 1 cut(s) 1057
HindIII AAGCTT 2 cut(s) 108, 651
HinfI GANTC 5 cut(s) 4, 117, 339, 659, 1473
HpaII CCGG 5 cut(s) 63, 70, 192, 554, 1386
HphI GGTGA 3 cut(s) 815, 1110, 1315
Hpy166II GTNNAC 4 cut(s) 373, 418, 979, 1057
Hpy188I TCNGA 5 cut(s) 106, 186, 588, 859, 1447
Hpy8I GTNNAC 4 cut(s) 373, 418, 979, 1057
Hpy99I CGWCG 2 cut(s) 158, 167
HpyAV CCTTC 6 cut(s) 111, 469, 686, 692, 1106, 1452
HpyCH4III ACNGT 3 cut(s) 163, 415, 836
HpyCH4IV ACGT 3 cut(s) 87, 369, 749
HpyF10VI GCNNNNNNNGC 4 cut(s) 59, 716, 725, 1386
HpyF3I CTNAG 1 cut(s) 956
HpySE526I ACGT 3 cut(s) 87, 369, 749
HspAI GCGC 3 cut(s) 18, 1063, 1437
KroI GCCGGC 3 cut(s) 191, 553, 1385
KroNI GCCGGC 3 cut(s) 193, 555, 1387
Ksp22I TGATCA 1 cut(s) 499
Kzo9I GATC 9 cut(s) 145, 186, 499, 523, 583, 1072, 1115, 1328, 1447
LmnI GCTCC 6 cut(s) 145, 200, 1111, 1399, 1455, 1463
Lsp1109I GCAGC 3 cut(s) 71, 418, 601
LweI GCATC 4 cut(s) 540, 678, 728, 1057
MaeI CTAG 1 cut(s) 330
MaeII ACGT 3 cut(s) 87, 369, 749
MaeIII GTNAC 4 cut(s) 365, 409, 803, 836
MalI GATC 9 cut(s) 147, 188, 501, 525, 585, 1074, 1117, 1330, 1449
MbiI CCGCTC 1 cut(s) 1270
MboI GATC 9 cut(s) 145, 186, 499, 523, 583, 1072, 1115, 1328, 1447
MboII GAAGA 8 cut(s) 268, 613, 616, 664, 677, 743, 842, 1035
MflI RGATCY 1 cut(s) 1328
MhlI GDGCHC 2 cut(s) 957, 1460
MlsI TGGCCA 3 cut(s) 14, 402, 1263
MluCI AATT 5 cut(s) 211, 540, 910, 1145, 1218
MluNI TGGCCA 3 cut(s) 14, 402, 1263
MlyI GAGTC 1 cut(s) 348
Mox20I TGGCCA 3 cut(s) 14, 402, 1263
MroNI GCCGGC 3 cut(s) 191, 553, 1385
MscI TGGCCA 3 cut(s) 14, 402, 1263
MseI TTAA 4 cut(s) 78, 348, 888, 1293
MslI CAYNNNNRTG 2 cut(s) 363, 1179
Msp20I TGGCCA 3 cut(s) 14, 402, 1263
MspA1I CMGCKG 1 cut(s) 1259
MspCI CTTAAG 1 cut(s) 1292
MspI CCGG 5 cut(s) 63, 70, 192, 554, 1386
MspR9I CCNGG 4 cut(s) 71, 537, 759, 1367
Mva1269I GAATGC 1 cut(s) 1176
MvaI CCWGG 3 cut(s) 537, 759, 1367
MvnI CGCG 1 cut(s) 1065
MwoI GCNNNNNNNGC 4 cut(s) 59, 716, 725, 1386
NaeI GCCGGC 3 cut(s) 193, 555, 1387
NciI CCSGG 1 cut(s) 71
NdeII GATC 9 cut(s) 145, 186, 499, 523, 583, 1072, 1115, 1328, 1447
NgoMIV GCCGGC 3 cut(s) 191, 553, 1385
NlaIV GGNNCC 7 cut(s) 74, 160, 169, 196, 622, 1107, 1330
NmeAIII GCCGAG 1 cut(s) 29
NmuCI GTSAC 3 cut(s) 365, 803, 836
NspI RCATGY 2 cut(s) 614, 1360
PaeI GCATGC 1 cut(s) 614
PcsI WCGNNNNNNNCGW 1 cut(s) 159
PctI GAATGC 1 cut(s) 1176
PdiI GCCGGC 3 cut(s) 193, 555, 1387
PfeI GAWTC 3 cut(s) 117, 659, 1473
PfoI TCCNGGA 1 cut(s) 535
PkrI GCNGC 4 cut(s) 61, 433, 616, 1269
PleI GAGTC 1 cut(s) 347
PpsI GAGTC 1 cut(s) 347
PpuMI RGGWCCY 1 cut(s) 73
Psp124BI GAGCTC 1 cut(s) 1460
Psp1406I AACGTT 1 cut(s) 87
Psp5II RGGWCCY 1 cut(s) 73
Psp6I CCWGG 3 cut(s) 535, 757, 1365
PspGI CCWGG 3 cut(s) 535, 757, 1365
PspN4I GGNNCC 7 cut(s) 74, 160, 169, 196, 622, 1107, 1330
PspPI GGNCC 7 cut(s) 73, 168, 491, 533, 679, 1021, 1229
PspPPI RGGWCCY 1 cut(s) 73
PstI CTGCAG 2 cut(s) 61, 619
PsuI RGATCY 1 cut(s) 1328
PvuII CAGCTG 1 cut(s) 1259
RsaI GTAC 3 cut(s) 299, 752, 980
RsaNI GTAC 3 cut(s) 298, 751, 979
RseI CAYNNNNRTG 2 cut(s) 363, 1179
SacI GAGCTC 1 cut(s) 1460
SaqAI TTAA 4 cut(s) 78, 348, 888, 1293
SatI GCNGC 4 cut(s) 60, 432, 615, 1268
Sau3AI GATC 9 cut(s) 145, 186, 499, 523, 583, 1072, 1115, 1328, 1447
Sau96I GGNCC 7 cut(s) 73, 168, 491, 533, 679, 1021, 1229
SchI GAGTC 1 cut(s) 348
ScrFI CCNGG 4 cut(s) 71, 537, 759, 1367
SduI GDGCHC 2 cut(s) 957, 1460
SfaNI GCATC 4 cut(s) 540, 678, 728, 1057
SfcI CTRYAG 2 cut(s) 57, 615
SinI GGWCC 5 cut(s) 73, 168, 491, 533, 1229
SmiMI CAYNNNNRTG 2 cut(s) 363, 1179
SmlI CTYRAG 2 cut(s) 1033, 1292
SmoI CTYRAG 2 cut(s) 1033, 1292
SphI GCATGC 1 cut(s) 614
Sse9I AATT 5 cut(s) 211, 540, 910, 1145, 1218
SsiI CCGC 4 cut(s) 356, 489, 1268, 1409
SspMI CTAG 1 cut(s) 330
SstI GAGCTC 1 cut(s) 1460
StyD4I CCNGG 4 cut(s) 69, 535, 757, 1365
StyI CCWWGG 3 cut(s) 329, 439, 1121
TaaI ACNGT 3 cut(s) 163, 415, 836
TaiI ACGT 3 cut(s) 90, 372, 752
TaqI TCGA 3 cut(s) 648, 675, 1252
TasI AATT 5 cut(s) 211, 540, 910, 1145, 1218
TatI WGTACW 1 cut(s) 978
TauI GCSGC 1 cut(s) 1270
TfiI GAWTC 3 cut(s) 117, 659, 1473
Tru1I TTAA 4 cut(s) 78, 348, 888, 1293
Tru9I TTAA 4 cut(s) 78, 348, 888, 1293
TscAI CASTG 4 cut(s) 403, 721, 841, 1483
TseFI GTSAC 3 cut(s) 365, 803, 836
TseI GCWGC 3 cut(s) 59, 431, 614
Tsp45I GTSAC 3 cut(s) 365, 803, 836
TspDTI ATGAA 9 cut(s) 283, 291, 499, 804, 894, 1070, 1197, 1205, 1364
TspGWI ACGGA 3 cut(s) 34, 96, 1359
TspRI CASTG 4 cut(s) 403, 721, 841, 1483
Vha464I CTTAAG 1 cut(s) 1292
VpaK11BI GGWCC 5 cut(s) 73, 168, 491, 533, 1229
XagI CCTNNNNNAGG 1 cut(s) 333
XapI RAATTY 2 cut(s) 540, 1145
XceI RCATGY 2 cut(s) 614, 1360
XmaJI CCTAGG 1 cut(s) 329
XspI CTAG 1 cut(s) 330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.