Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0003293

Physical Location & Seq

Genomic Coordinates Forward (+)

24950749 .. 24956355

Gene Structure 5607 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g33180.t10

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3045 bp

ATGGAGAGCTATTTGAACGAGAATTTTGATCTGAAGGCGAAGAACTCATCGGACGAGGCGCTTCAGAGATGGAGGAATCTTTGCTGGGTTGTGAAGAACAAGAAGAGGAGGTTTCGATTCACTGCTAATCTCGACAAGCGTTCTGAGGCTGAGACTATTCGACGCTCTAATCAGGAGAAGTTTAGACTTGCAATCTTGGTTTCGCAAGCTGCTCTTCAGTTCATCCAAGGGCTAAAATTTTCCAGTGATTACACTGTACCTGAGGAAGTCAAGGCTGCAGGTTTTCAAATTGATGCTGATGAGTTGGCATCCATTGTTGAAGGTCGTGATGTGAAGAAGCTCAAAATGCATGATGGTGTTGAGGGACTTGTAGGCAAGCTTGGAACATCAACTGTCGATGGCATTTCTACGTCAGAGCAGTTGCTTAATACGAGAAAGGAAATTTATGGAGTTAATAAATTCACTGAGAGCCCATCTCGTGGATTTTTGCTATTTGTATGGGAAGCCCTACAAGACACCACCCTCATGATACTTGGAGTTTGTGCCTTTGTCTCTCTGATTGTTGGGATAATGACAGAAGGATGGCCAAAGGGTGCCCATGATGGACTGGGAATTGTTGCCAGCATTTTGCTTGTTGTATTTGTGACTGCCACCAGTGATTATAAACAGTCCCTGCAGTTCAAGGACTTGGACAAGGAAAAGAAAAAGATTACAATTCATGTTACGAGAAATGGATTCAGACAGAAGTTGTCAATATATGATTTACTTCCTGGTGACATTGTTCATCTCAATATTGGAGATCTGGTCCCAGCTGATGGACTTTTCATTTCTGGCTTTTCTATGCTGATCAACGAGTCCAGCTTAACTGGAGAGAGTGAACCGGTTAACATTAATGATGTCAATCCTTTTCTCCTTTCTGGAACTAAAGTTCAAGATGGATCCTGCAAGATGCTTGTGACTACTGTCGGAATGAGAACCCAGTGGGGTAAATTGATGGCTACTCTTACTGAAGGTGGAGACGATGAAACCCCCTTGCAGGTGAAACTAAACGGTGTGGCAACTATCATTGGTAAAATAGGCCTGTTCTTCGCTGTAGTGACATTCGCTGTATTAGTTCAAGGATTGTTCAGCCGCAAGCTGCAACAAGGACTAACATGGTCTGGAGATGATGCAGTTGTTATATTGGAATTTTTCGCTATAGCTGTTACAATTGTAGTTGTTGCTGTTCCTGAAGGTTTGCCTTTGGCTGTAACATTAAGCCTTGCTTTTGCTATGAAGAAGATGATGAACGATAAGGCACTAGTCCGACATTTGGCTGCTTGTGAGACAATGGGATCGGCGACAACTATCTGTAGTGACAAGACTGGAACTCTAACTACGAACCGGATGACGGTTGTAAAAACTTGCATATGTTTGAAAATCAAAGACGTGGCTAGCTCTACCAAATCATCTGACCTCTGCTCTGAAATACCTGATTCTACTTTGAAAGTTCTACTTCAGTGCATATTTAACAATACTGGAGGAGATATTGTTAAAAACAAAGATGACAGGATTGAGATACTGGGAACACCCACTGATGCAGCTGTTCTGGGATTTGGGATGTCACTCGGTGGAGATTTCAAGGCAGAACGACAAGCATCAAAACTTGTCAAAGTTGAGCCCTTCAATTCCACTAAGAAGCGGATGGCTGTAGTTCTACAGCTTCCTGAAGGTGGCTTCCGAGTGTATTGTAAAGGTGCTTCCGAGATAATTTTAGCTGCTTGTGACAAATACATGGGTCCAAATGGCGAGGTAGTTCCCCTTGATATAGCTTCCATCAATCTTTTGAATGGTATCATAGAACAGTTTGCTAGTGAAGCCCTCAGAACTCTGTGCCTGGCATACATGGACATTGGAAGTGACTTCTCTGCTGAAAGTCCTATTCCTCTCAATGGATATACATGTATAGGAATTGTGGGTATTAAAGATCCAGTTCGTCCTGGTGTCAAGGAGTCTGTTGAGATTTGTAGGTCTGCTGGTATTACTGTCCGAATGGTGACTGGTGATAACATAAACACTGCAAAAGCAATTGCAAGAGAATGCGGAATTTTGACTGATGGAGGTTTAGCAATTGAAGGCCCAGAATTTCGTGAGAAGAGTGAGGAAGAATTACAAAAAATTATTCCCAAACTTCAGGTAATGGCTCGATCTTCGCCAATGGATAAGCATACTCTTGTAAAACACTTAAGAACCACTTTTGGAGAAGTTGTTGCAGTGACAGGTGATGGTACAAATGATGCTCCAGCACTTCATGAAGCTGATATAGGACTTGCAATGGGAATTGCTGGAACTGAGGTGGCTAAAGAGAGCGCTGATGTGATAATTCTGGACGATAACTTCTCTACAATTGTGACTGTGGCCAAATGGGGACGTTCAGTTTACTTGAACATACAGAAATTTGTTCAGTTCCAGTTAACAGTTAATGTGGTTGCCCTTGTAGTCAACTTTACTTCAGCATGTTTAACTGGAAGTGCTCCCCTTACCGCTGTTCAGCTTCTCTGGGTCAACATGATCATGGACACTCTAGGAGCCCTTGCACTAGCCACTGAGCCTCCTAATGATGATTTGATGAAGAGAACACCAGTTGGGAGGACAGGAAACTTCATTACTAATGTGATGTGGAGAAATATCTTGGGGCAGTCTCTGTATCAGTTTGTAGTAATATGGTATCTGCAGACTAAAGGAAAAGAAGCTTTTCAGCTTGTTGGCCCAGATTCAGATTTGATATTAAACACCCTGATTTTCAACTCATTTGTCTTCTGCCAGGCTTTCAATGAGATCAGTTCTAGAGAAATGGAGAAGATCAATGTCTTCAAAGGCATACTTCAGAACTACGTGTTTGTGACAGTTCTATCTTGCACTGTAATCTTCCAAGTCATAATCATCGAGTTCCTGGGTACATTTGCAAGTACATCTCCTCTCACTTGGCAGCAATGGTTTGTCAGTGTTACACTTGGATTTCTCGGCATGCCTATTTCAGCAGCTTTGAAATTTATTCCCGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005216 GO:0005261 GO:0005262 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0009507 GO:0009526 GO:0009528 GO:0009536 GO:0009706 GO:0009941 GO:0012505 GO:0015075 GO:0015085 GO:0015267 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0019866 GO:0022803 GO:0022804 GO:0022838 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031224 GO:0031226 GO:0031967 GO:0031969 GO:0031975 GO:0034220 GO:0042170 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase Hydrolase_3

Protein Analysis

1015

Amino Acids

110.62

Weight (kDa)

5.8

Isoelectric Point (pI)

39.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CaATP_NAI	PF12515	5 - 49	3.1e-20	Ca2+-ATPase N terminal autoinhibitory domain
Cation_ATPase_N	PF00690	118 - 185	1.5e-09	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	237 - 334	2.1e-19	P-type ATPase actuator domain
Cation_ATPase	PF13246	501 - 660	4.1e-31	P-type ATPase, cytoplasmic domain N
Hydrolase	PF00702	645 - 767	9e-12	haloacid dehalogenase-like hydrolase
Cation_ATPase_C	PF00689	838 - 938	2.9e-25	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003293)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G27770 AT1G27770 AT1G27770 AT1G27770
fragaria_vesca	FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180
malus_domestica	MD08G1085200.v1.1 MD08G1085600.v1.1 MD12G1068300.v1.1 MD15G1070800.v1.1
prunus_persica	Prupe.1G423500_v2.0.a1 Prupe.1G423500_v2.0.a1 Prupe.1G423500_v2.0.a1 Prupe.1G423500_v2.0.a1 Prupe.1G423500_v2.0.a1
pyrus_communis	pycom08g07020 pycom15g06650 pycom15g06660
rosa_chinensis	RchiOBHm_Chr6g0309491
rosa_laevigata	RLG00000010561
rosa_multiflora	Rmu_sc0007283.1_g000007
rosa_roxburghii	Rroxscaffold_7G00159360
rosa_rugosa	Rorug06G0372300 Rorug06G0372400
rosa_samantha	Rh6AG485700 Rh6BG495000
rosa_wichuraiana	Rw6G042300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	663
AarI	CACCTGC	1 cut(s)	1027
Acc36I	ACCTGC	2 cut(s)	269, 1027
AccB1I	GGYRCC	1 cut(s)	593
AccB7I	CCANNNNNTGG	2 cut(s)	479, 815
AciI	CCGC	4 cut(s)	1132, 1681, 2082, 2523
AclWI	GGATC	4 cut(s)	933, 946, 1342, 1961
AcoI	YGGCCR	2 cut(s)	584, 2397
AcsI	RAATTY	9 cut(s)	22, 236, 441, 458, 1187, 2085, 2123, 2435, 3029
AdeI	CACNNNGTG	1 cut(s)	1610
AfaI	GTAC	4 cut(s)	258, 2269, 2938, 2950
AfeI	AGCGCT	1 cut(s)	2350
AfiI	CCNNNNNNNGG	7 cut(s)	479, 815, 1036, 1312, 1390, 1712, 1931
AflII	CTTAAG	1 cut(s)	2224
AflIII	ACRYGT	2 cut(s)	1940, 2874
AgeI	ACCGGT	1 cut(s)	880
AhlI	ACTAGT	1 cut(s)	1300
AjiI	CACGTC	1 cut(s)	1429
AjnI	CCWGG	5 cut(s)	769, 1875, 1978, 2802, 2931
AloI	GAACNNNNNNTCC	2 cut(s)	912, 944
Alw21I	GWGCWC	1 cut(s)	2515
Alw26I	GTCTC	5 cut(s)	146, 556, 1011, 1319, 2685
AlwI	GGATC	4 cut(s)	933, 946, 1342, 1961
AlwNI	CAGNNNCTG	2 cut(s)	673, 2683
Aor51HI	AGCGCT	1 cut(s)	2350
AoxI	GGCC	5 cut(s)	584, 1078, 2116, 2397, 2746
ApeKI	GCWGC	8 cut(s)	209, 275, 1138, 1316, 1580, 1757, 2968, 3020
ApoI	RAATTY	9 cut(s)	22, 236, 441, 458, 1187, 2085, 2123, 2435, 3029
AseI	ATTAAT	1 cut(s)	891
AsiGI	ACCGGT	1 cut(s)	880
Asp700I	GAANNNNTTC	1 cut(s)	2733
AspLEI	GCGC	2 cut(s)	61, 2351
AspS9I	GGNCC	4 cut(s)	805, 1778, 2117, 2747
AsuHPI	GGTGA	5 cut(s)	785, 1051, 2047, 2054, 2273
AsuNHI	GCTAGC	1 cut(s)	1433
AvaII	GGWCC	2 cut(s)	805, 1778
AxyI	CCTNAGG	1 cut(s)	261
BaeGI	GKGCMC	1 cut(s)	598
BalI	TGGCCA	2 cut(s)	586, 2399
BamHI	GGATCC	1 cut(s)	938
BanI	GGYRCC	1 cut(s)	593
BanII	GRGCYC	3 cut(s)	473, 1662, 2572
BarI	GAAGNNNNNNTAC	2 cut(s)	1723, 1755
BauI	CACGAG	1 cut(s)	477
BbsI	GAAGAC	2 cut(s)	2788, 2842
Bbv12I	GWGCWC	1 cut(s)	2515
BbvI	GCAGC	8 cut(s)	196, 262, 1125, 1303, 1592, 1744, 2980, 3032
BciT130I	CCWGG	5 cut(s)	771, 1877, 1980, 2804, 2933
BclI	TGATCA	2 cut(s)	846, 2550
BcoDI	GTCTC	5 cut(s)	146, 556, 1011, 1319, 2685
BcuI	ACTAGT	1 cut(s)	1300
BfaI	CTAG	6 cut(s)	1301, 1434, 1851, 2564, 2579, 2826
BfmI	CTRYAG	8 cut(s)	276, 674, 1092, 1197, 1351, 1689, 1697, 2711
BfoI	RGCGCY	2 cut(s)	62, 2352
BfrI	CTTAAG	1 cut(s)	2224
BfuAI	ACCTGC	2 cut(s)	269, 1027
BglII	AGATCT	1 cut(s)	799
BisI	GCNGC	9 cut(s)	210, 276, 1132, 1139, 1317, 1581, 1758, 2969, 3021
BlsI	GCNGC	9 cut(s)	211, 277, 1133, 1140, 1318, 1582, 1759, 2970, 3022
Bme1390I	CCNGG	5 cut(s)	771, 1877, 1980, 2804, 2933
Bme18I	GGWCC	2 cut(s)	805, 1778
BmgBI	CACGTC	1 cut(s)	1429
BmgT120I	GGNCC	4 cut(s)	805, 1778, 2117, 2747
BmiI	GGNNCC	5 cut(s)	595, 807, 940, 1779, 2569
BmrFI	CCNGG	5 cut(s)	771, 1877, 1980, 2804, 2933
BmrI	ACTGGG	3 cut(s)	617, 973, 1571
BmsI	GCATC	7 cut(s)	283, 317, 939, 1159, 1567, 1646, 2266
BmtI	GCTAGC	1 cut(s)	1437
BmuI	ACTGGG	3 cut(s)	617, 973, 1571
BoxI	GACNNNNGTC	1 cut(s)	962
BpiI	GAAGAC	2 cut(s)	2788, 2842
BplI	GAGNNNNNCTC	2 cut(s)	460, 492
BpmI	CTGGAG	4 cut(s)	888, 1182, 1539, 2265
BsaAI	YACGTR	1 cut(s)	2875
BsaBI	GATNNNNATC	1 cut(s)	900
BsaJI	CCNNGG	2 cut(s)	226, 2932
BsaWI	WCCGGW	2 cut(s)	880, 1383
BsaXI	ACNNNNNCTCC	8 cut(s)	789, 819, 1515, 1545, 2232, 2262, 2575, 2605
Bsc4I	CCNNNNNNNGG	7 cut(s)	479, 815, 1036, 1312, 1390, 1712, 1931
Bse118I	RCCGGY	1 cut(s)	880
Bse21I	CCTNAGG	1 cut(s)	261
Bse3DI	GCAATG	2 cut(s)	2319, 2978
Bse8I	GATNNNNATC	1 cut(s)	900
BseBI	CCWGG	5 cut(s)	771, 1877, 1980, 2804, 2933
BseDI	CCNNGG	2 cut(s)	226, 2932
BseGI	GGATG	6 cut(s)	222, 308, 587, 1392, 1605, 1689
BseJI	GATNNNNATC	1 cut(s)	900
BseLI	CCNNNNNNNGG	7 cut(s)	479, 815, 1036, 1312, 1390, 1712, 1931
BseMI	GCAATG	2 cut(s)	2319, 2978
BseMII	CTCAG	7 cut(s)	135, 141, 252, 456, 1876, 2322, 2577
BseRI	GAGGAG	3 cut(s)	121, 1536, 2946
BseSI	GKGCMC	1 cut(s)	598
BseXI	GCAGC	8 cut(s)	196, 262, 1125, 1303, 1592, 1744, 2980, 3032
BseYI	CCCAGC	2 cut(s)	84, 808
BshFI	GGCC	5 cut(s)	586, 1080, 2118, 2399, 2748
BshNI	GGYRCC	1 cut(s)	593
BshTI	ACCGGT	1 cut(s)	880
BsiHKAI	GWGCWC	1 cut(s)	2515
BsiSI	CCGG	2 cut(s)	881, 1384
BslFI	GGGAC	4 cut(s)	378, 655, 791, 2421
BslI	CCNNNNNNNGG	7 cut(s)	479, 815, 1036, 1312, 1390, 1712, 1931
BsmAI	GTCTC	5 cut(s)	146, 556, 1011, 1319, 2685
BsmBI	CGTCTC	1 cut(s)	1011
BsmFI	GGGAC	4 cut(s)	378, 655, 791, 2421
BsmI	GAATGC	1 cut(s)	2084
BsnI	GGCC	5 cut(s)	586, 1080, 2118, 2399, 2748
Bsp1286I	GDGCHC	5 cut(s)	473, 598, 1662, 2515, 2572
BspACI	CCGC	4 cut(s)	1132, 1681, 2082, 2523
BspANI	GGCC	5 cut(s)	586, 1080, 2118, 2399, 2748
BspCNI	CTCAG	7 cut(s)	136, 142, 253, 457, 1875, 2323, 2578
BspHI	TCATGA	2 cut(s)	525, 2290
BspLI	GGNNCC	5 cut(s)	595, 807, 940, 1779, 2569
BspMAI	CTGCAG	3 cut(s)	280, 678, 2715
BspMI	ACCTGC	2 cut(s)	269, 1027
BspOI	GCTAGC	1 cut(s)	1437
BspPI	GGATC	4 cut(s)	933, 946, 1342, 1961
BspQI	GCTCTTC	1 cut(s)	219
BspT107I	GGYRCC	1 cut(s)	593
BspTI	CTTAAG	1 cut(s)	2224
BsrDI	GCAATG	2 cut(s)	2319, 2978
BsrFI	RCCGGY	1 cut(s)	880
BssAI	RCCGGY	1 cut(s)	880
BssECI	CCNNGG	2 cut(s)	226, 2932
BssSI	CACGAG	1 cut(s)	477
BssT1I	CCWWGG	1 cut(s)	226
Bst2BI	CACGAG	1 cut(s)	477
Bst2UI	CCWGG	5 cut(s)	771, 1877, 1980, 2804, 2933
Bst6I	CTCTTC	4 cut(s)	98, 219, 2129, 2606
BstAFI	CTTAAG	1 cut(s)	2224
BstBAI	YACGTR	1 cut(s)	2875
BstC8I	GCNNGC	6 cut(s)	207, 377, 622, 1136, 1435, 3008
BstDEI	CTNAG	8 cut(s)	144, 150, 261, 465, 1674, 1862, 2331, 2586
BstF5I	GGATG	6 cut(s)	222, 308, 587, 1392, 1605, 1689
BstH2I	RGCGCY	2 cut(s)	62, 2352
BstHHI	GCGC	2 cut(s)	61, 2351
BstMAI	GTCTC	5 cut(s)	146, 556, 1011, 1319, 2685
BstMWI	GCNNNNNNNGC	2 cut(s)	346, 1856
BstNI	CCWGG	5 cut(s)	771, 1877, 1980, 2804, 2933
BstNSI	RCATGY	3 cut(s)	1944, 2499, 3010
BstPAI	GACNNNNGTC	1 cut(s)	962
BstSCI	CCNGG	5 cut(s)	769, 1875, 1978, 2802, 2931
BstSFI	CTRYAG	8 cut(s)	276, 674, 1092, 1197, 1351, 1689, 1697, 2711
BstSLI	GKGCMC	1 cut(s)	598
BstV1I	GCAGC	8 cut(s)	196, 262, 1125, 1303, 1592, 1744, 2980, 3032
BstV2I	GAAGAC	2 cut(s)	2788, 2842
BstX2I	RGATCY	3 cut(s)	799, 938, 1966
BstYI	RGATCY	3 cut(s)	799, 938, 1966
Bsu36I	CCTNAGG	1 cut(s)	261
BsuRI	GGCC	5 cut(s)	586, 1080, 2118, 2399, 2748
BtrI	CACGTC	1 cut(s)	1429
BtsCI	GGATG	6 cut(s)	222, 308, 587, 1392, 1605, 1689
BtsI	GCAGTG	3 cut(s)	120, 2055, 2259
BveI	ACCTGC	2 cut(s)	269, 1027
Cac8I	GCNNGC	6 cut(s)	207, 377, 622, 1136, 1435, 3008
CaiI	CAGNNNCTG	2 cut(s)	673, 2683
CciI	TCATGA	2 cut(s)	525, 2290
CfoI	GCGC	2 cut(s)	61, 2351
Cfr10I	RCCGGY	1 cut(s)	880
Cfr13I	GGNCC	4 cut(s)	805, 1778, 2117, 2747
CseI	GACGC	1 cut(s)	171
Csp6I	GTAC	4 cut(s)	257, 2268, 2937, 2949
CspAI	ACCGGT	1 cut(s)	880
CviQI	GTAC	4 cut(s)	257, 2268, 2937, 2949
DdeI	CTNAG	8 cut(s)	144, 150, 261, 465, 1674, 1862, 2331, 2586
DraIII	CACNNNGTG	1 cut(s)	1610
EaeI	YGGCCR	2 cut(s)	584, 2397
Eam1104I	CTCTTC	4 cut(s)	98, 219, 2129, 2606
EarI	CTCTTC	4 cut(s)	98, 219, 2129, 2606
Eco130I	CCWWGG	1 cut(s)	226
Eco147I	AGGCCT	1 cut(s)	1080
Eco24I	GRGCYC	3 cut(s)	473, 1662, 2572
Eco47I	GGWCC	2 cut(s)	805, 1778
Eco47III	AGCGCT	1 cut(s)	2350
Eco81I	CCTNAGG	1 cut(s)	261
EcoRII	CCWGG	5 cut(s)	769, 1875, 1978, 2802, 2931
EcoT14I	CCWWGG	1 cut(s)	226
EcoT22I	ATGCAT	1 cut(s)	351
EcoT38I	GRGCYC	3 cut(s)	473, 1662, 2572
ErhI	CCWWGG	1 cut(s)	226
Esp3I	CGTCTC	1 cut(s)	1011
FaqI	GGGAC	4 cut(s)	378, 655, 791, 2421
FauNDI	CATATG	1 cut(s)	1409
FbaI	TGATCA	2 cut(s)	846, 2550
Fnu4HI	GCNGC	9 cut(s)	210, 276, 1132, 1139, 1317, 1581, 1758, 2969, 3021
FokI	GGATG	6 cut(s)	209, 295, 594, 1399, 1612, 1696
FriOI	GRGCYC	3 cut(s)	473, 1662, 2572
Fsp4HI	GCNGC	9 cut(s)	210, 276, 1132, 1139, 1317, 1581, 1758, 2969, 3021
FspBI	CTAG	6 cut(s)	1301, 1434, 1851, 2564, 2579, 2826
GlaI	GCGC	2 cut(s)	60, 2350
GluI	GCNGC	9 cut(s)	210, 276, 1132, 1139, 1317, 1581, 1758, 2969, 3021
GsaI	CCCAGC	2 cut(s)	88, 812
GsuI	CTGGAG	4 cut(s)	888, 1182, 1539, 2265
HaeII	RGCGCY	2 cut(s)	62, 2352
HaeIII	GGCC	5 cut(s)	586, 1080, 2118, 2399, 2748
HapII	CCGG	2 cut(s)	881, 1384
HgaI	GACGC	1 cut(s)	171
HhaI	GCGC	2 cut(s)	61, 2351
Hin6I	GCGC	2 cut(s)	59, 2349
HinP1I	GCGC	2 cut(s)	59, 2349
HincII	GTYRAC	4 cut(s)	886, 2454, 2482, 2545
HindII	GTYRAC	4 cut(s)	886, 2454, 2482, 2545
HindIII	AAGCTT	2 cut(s)	377, 2730
HinfI	GANTC	7 cut(s)	76, 117, 735, 854, 1475, 1991, 2753
HpaI	GTTAAC	2 cut(s)	886, 2454
HpaII	CCGG	2 cut(s)	881, 1384
HphI	GGTGA	5 cut(s)	785, 1051, 2047, 2054, 2273
Hpy166II	GTNNAC	6 cut(s)	878, 886, 2419, 2454, 2482, 2545
Hpy8I	GTNNAC	6 cut(s)	878, 886, 2419, 2454, 2482, 2545
Hpy99I	CGWCG	1 cut(s)	165
HpyAV	CCTTC	8 cut(s)	28, 314, 572, 1004, 1226, 1672, 1703, 2108
HpyCH4IV	ACGT	4 cut(s)	410, 1428, 2410, 2874
HpyF10VI	GCNNNNNNNGC	2 cut(s)	346, 1856
HpyF3I	CTNAG	8 cut(s)	144, 150, 261, 465, 1674, 1862, 2331, 2586
HpySE526I	ACGT	4 cut(s)	410, 1428, 2410, 2874
HspAI	GCGC	2 cut(s)	59, 2349
Ksp22I	TGATCA	2 cut(s)	846, 2550
KspAI	GTTAAC	2 cut(s)	886, 2454
LguI	GCTCTTC	1 cut(s)	219
LmnI	GCTCC	3 cut(s)	2284, 2518, 2567
Lsp1109I	GCAGC	8 cut(s)	196, 262, 1125, 1303, 1592, 1744, 2980, 3032
LweI	GCATC	7 cut(s)	283, 317, 939, 1159, 1567, 1646, 2266
MaeI	CTAG	6 cut(s)	1301, 1434, 1851, 2564, 2579, 2826
MaeII	ACGT	4 cut(s)	410, 1428, 2410, 2874
MfeI	CAATTG	4 cut(s)	1209, 2067, 2109, 2385
MflI	RGATCY	3 cut(s)	799, 938, 1966
MhlI	GDGCHC	5 cut(s)	473, 598, 1662, 2515, 2572
MlsI	TGGCCA	2 cut(s)	586, 2399
MluNI	TGGCCA	2 cut(s)	586, 2399
MlyI	GAGTC	2 cut(s)	863, 2000
MmeI	TCCRAC	2 cut(s)	946, 1330
Mox20I	TGGCCA	2 cut(s)	586, 2399
Mph1103I	ATGCAT	1 cut(s)	351
MroXI	GAANNNNTTC	1 cut(s)	2733
MscI	TGGCCA	2 cut(s)	586, 2399
MslI	CAYNNNNRTG	3 cut(s)	354, 524, 2552
Msp20I	TGGCCA	2 cut(s)	586, 2399
MspA1I	CMGCKG	3 cut(s)	812, 1583, 2525
MspCI	CTTAAG	1 cut(s)	2224
MspI	CCGG	2 cut(s)	881, 1384
MspR9I	CCNGG	5 cut(s)	771, 1877, 1980, 2804, 2933
MunI	CAATTG	4 cut(s)	1209, 2067, 2109, 2385
Mva1269I	GAATGC	1 cut(s)	2084
MvaI	CCWGG	5 cut(s)	771, 1877, 1980, 2804, 2933
MwoI	GCNNNNNNNGC	2 cut(s)	346, 1856
NdeI	CATATG	1 cut(s)	1409
NheI	GCTAGC	1 cut(s)	1433
NlaIV	GGNNCC	5 cut(s)	595, 807, 940, 1779, 2569
NmeAIII	GCCGAG	1 cut(s)	2982
NsiI	ATGCAT	1 cut(s)	351
NspI	RCATGY	3 cut(s)	1944, 2499, 3010
PaeI	GCATGC	1 cut(s)	3010
PagI	TCATGA	2 cut(s)	525, 2290
PaqCI	CACCTGC	1 cut(s)	1027
PceI	AGGCCT	1 cut(s)	1080
PciI	ACATGT	1 cut(s)	1940
PciSI	GCTCTTC	1 cut(s)	219
PctI	GAATGC	1 cut(s)	2084
PdmI	GAANNNNTTC	1 cut(s)	2733
PfeI	GAWTC	5 cut(s)	76, 117, 735, 1475, 2753
PflMI	CCANNNNNTGG	2 cut(s)	479, 815
PinAI	ACCGGT	1 cut(s)	880
PkrI	GCNGC	9 cut(s)	211, 277, 1133, 1140, 1318, 1582, 1759, 2970, 3022
PleI	GAGTC	2 cut(s)	862, 1999
PpsI	GAGTC	2 cut(s)	862, 1999
Ppu21I	YACGTR	1 cut(s)	2875
PscI	ACATGT	1 cut(s)	1940
PshAI	GACNNNNGTC	1 cut(s)	962
PshBI	ATTAAT	1 cut(s)	891
PsiI	TTATAA	1 cut(s)	663
Psp6I	CCWGG	5 cut(s)	769, 1875, 1978, 2802, 2931
PspFI	CCCAGC	2 cut(s)	84, 808
PspGI	CCWGG	5 cut(s)	769, 1875, 1978, 2802, 2931
PspN4I	GGNNCC	5 cut(s)	595, 807, 940, 1779, 2569
PspPI	GGNCC	4 cut(s)	805, 1778, 2117, 2747
PsrI	GAACNNNNNNTAC	2 cut(s)	1360, 1392
PstI	CTGCAG	3 cut(s)	280, 678, 2715
PstNI	CAGNNNCTG	2 cut(s)	673, 2683
PsuI	RGATCY	3 cut(s)	799, 938, 1966
PvuII	CAGCTG	2 cut(s)	812, 1583
RsaI	GTAC	4 cut(s)	258, 2269, 2938, 2950
RsaNI	GTAC	4 cut(s)	257, 2268, 2937, 2949
RseI	CAYNNNNRTG	3 cut(s)	354, 524, 2552
SapI	GCTCTTC	1 cut(s)	219
SatI	GCNGC	9 cut(s)	210, 276, 1132, 1139, 1317, 1581, 1758, 2969, 3021
Sau96I	GGNCC	4 cut(s)	805, 1778, 2117, 2747
SchI	GAGTC	2 cut(s)	863, 2000
ScrFI	CCNGG	5 cut(s)	771, 1877, 1980, 2804, 2933
SduI	GDGCHC	5 cut(s)	473, 598, 1662, 2515, 2572
SfaNI	GCATC	7 cut(s)	283, 317, 939, 1159, 1567, 1646, 2266
SfcI	CTRYAG	8 cut(s)	276, 674, 1092, 1197, 1351, 1689, 1697, 2711
SinI	GGWCC	2 cut(s)	805, 1778
SmiMI	CAYNNNNRTG	3 cut(s)	354, 524, 2552
SmlI	CTYRAG	1 cut(s)	2224
SmoI	CTYRAG	1 cut(s)	2224
SpeI	ACTAGT	1 cut(s)	1300
SphI	GCATGC	1 cut(s)	3010
SseBI	AGGCCT	1 cut(s)	1080
SsiI	CCGC	4 cut(s)	1132, 1681, 2082, 2523
SspI	AATATT	1 cut(s)	793
SspMI	CTAG	6 cut(s)	1301, 1434, 1851, 2564, 2579, 2826
StuI	AGGCCT	1 cut(s)	1080
StyD4I	CCNGG	5 cut(s)	769, 1875, 1978, 2802, 2931
StyI	CCWWGG	1 cut(s)	226
TaiI	ACGT	4 cut(s)	413, 1431, 2413, 2877
TaqI	TCGA	6 cut(s)	115, 132, 160, 396, 2185, 2925
TatI	WGTACW	1 cut(s)	2948
TauI	GCSGC	1 cut(s)	1134
TfiI	GAWTC	5 cut(s)	76, 117, 735, 1475, 2753
TseI	GCWGC	8 cut(s)	209, 275, 1138, 1316, 1580, 1757, 2968, 3020
Van91I	CCANNNNNTGG	2 cut(s)	479, 815
Vha464I	CTTAAG	1 cut(s)	2224
VpaK11BI	GGWCC	2 cut(s)	805, 1778
VspI	ATTAAT	1 cut(s)	891
XapI	RAATTY	9 cut(s)	22, 236, 441, 458, 1187, 2085, 2123, 2435, 3029
XbaI	TCTAGA	1 cut(s)	2825
XceI	RCATGY	3 cut(s)	1944, 2499, 3010
XmnI	GAANNNNTTC	1 cut(s)	2733
XspI	CTAG	6 cut(s)	1301, 1434, 1851, 2564, 2579, 2826
Zsp2I	ATGCAT	1 cut(s)	351

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.