Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0003293

Physical Location & Seq

Genomic Coordinates Forward (+)

4840553 .. 4846142

Gene Structure 5590 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1070800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3051 bp

ATGGAGAACTATTTGAACGAGAACTTCGATGTGAAGGCGAAGAACTCGTCGGAGGAGGCGCTTCAGAGATGGAGGAGGCTCTGTGGATTTGTGAAGAATCGGAAACGGAGGTTCAGATTCACTGCTAATTTCTCCAAGCGGTTTGAGGCCGAGGTCATTCGACGCTCCAATCATGAGAAGTTTAGAGTTGCAGTTTTGGTTTCACAAGCTGCGCTTCAGTTTATCCATGGCTTAAGTTTGCCCAGTGATTATACCGTACCTGAGGAAGTCAAAGCTGCGGGGTTTCAAATTTGTGCTGATGAGGTTGGATCGATTGTTGAAGGCCGTGATGTGAAAAAGCTGAAAAGTCATGGTGGTGTGGAGACTATCACAGACAAGCTTGGAACATCAAGTATCAATGGAATTTCTACTTCTGAGAAAATGCTGCATAAGAGAAAAGAAATATATGGAGTTAATAAATTTGCTGAAAAACCATCCCGTGGATTTCTTGTTTATGTGTGGGAAGCCCTTCAAGACACTACCCTCATGATACTTGCATTTTGTGCCCTTGTATCTCTGCTTGTTGGGATAATGACAGAAGGTTGGCCAAAGGGTGCCCACGATGGACTTGGAATTGTTGCGAGCATTTTGCTTGTTGTATTTGTCACTGCAACTAGTGATTATAAGCAATCCCTGCAGTTCAAGGACTTGGAGAAGGAGAAGAAAAAAATTACAGTTCAGGCTACTAGAGATGGATTCAGACAGAAGCTGTCCATATATGAACTACTTCCTGGTGACATTGTTCATCTTTCTATTGGAGATCTGGTCCCAGCTGATGGACTTTTTGTTTCTGGGTTTTCTGTGCTGATAAATGAATCCAGTTTAACTGGAGAGAGTGAACCGGTTAAAGTCAATTCTGATCATCCTTTTCTCCTGTCTGGAACTAAAGTTCAAGATGGATCATGCAAGATGCTTGTAACTACGGTTGGAATGAGAACCCAGTGGGGTAAACTGATGGCTACTCTCAGTGAAGGAGGAGATGACGAAACCCCCTTGCAGGTTAAACTAAATGGTGTGGCAAGCATCATTGGGAAAATAGGCCTGTTTTTTGCTGTTGTGACATTTTCTGTGTTGGTTCAAGGATTGTTCAGCCGCAAGCTTCAAGAAGGATCCCACTTGATCTGGTCTGGAGATGACGCATTGGAAATCTTGGAATATTTTGCTATAGCTGTTACAATTGTAGTTGTTGCTGTTCCCGAAGGTTTGCCTTTGGCTGTGACATTGAGTCTTGCTTTTGCCATGAAGAAGATGATGAACGATAAGGCACTAGTCAGGCATTTGGCCGCTTGTGAGACAATGGGATCAGCCACAACTATCTGCAGTGACAAGACTGGGACACTAACAACTAACCACATGACTGTTGTGAAAGCTTGCATTTGTGGGAAAATCAGAGATGTGGGAACCTCTGAGGAAGCTTCTAACTTGGCCTCCATAGTTCCTGGCGCTGGTTTGAGACTCATACTTCAATCTATTTTTAACAACACTGGAGGAGAAGTTGTTAAAAACAAAGATGGCAAGATTGAGTTACTTGGAACACCCACTGAGACTGCTATTTTGGAATTTGGGATGTTGCTTGGCGGTGATTGCAAGGCAGAACGGGAAGCATCAAAAGTTGTTAAAATTGAGCCCTTCAATTCTCATAAGAAGCGAATGGGTGTTGTTCTAGAGCTTCCTGAAGGTGGCTTCCGGGTGCACACTAAAGGTGCTTCTGAGATAATATTAGCTGCATGTGACAAATTCATGGGCCCAGATGGCGAGGTTGTTCCACTCGATAAAGCATCCATTGATCTTTTCAGTGGCATCATTAAACGTTTTGCTAGTGAAGCCCTTAGAACTCTCTGCCTTGCATACATGGAGGTTGGCAATGAGTTCTCAGCTGAAAGTCCTATTCCCGCCTCAGGGTACACGTGTATAGGCATTGTGGGTATCAAAGATCCAGTTCGTCCTGGTGTCAAGGAGTCCGTTGCAATTTGTAGGTCTGCTGGTATTACAGTCAGAATGGTTACTGGAGACAACATAAACACTGCAAAAGCAATTGCAATAGAATGTGGAATTTTGACCGATGGGGGTTTGGCAATTGAAGGCCCAGAATTTCGTGAGAAGAGTGAGGAGGAATTGCAAAAAATTATTCCAGAACTTCAGGTTATGGCTCGATCTTCACCAATGGATAAGCATACCCTAGTAAGACACCTAAGGACCACTTTTCAAGAAGTTGTTGCCGTGACAGGTGATGGTACAAACGATGCTCCAGCACTTCATGAAGCTGATATAGGGCTTGCAATGGGCATTGCTGGAACTGAGGTAGCAAAAGAAAGTGCTGATGTGATAATTCTGGATGATAACTTCTCTACAATTGTGACTGTCGCCAAATGGGGACGTTCAGTGTACTTGAACATACAGAAATTTGTTCAGTTCCAGTTAACGGTTAATGTGGTTGCACTTATTGTCAACTTTTCTTCTGCCTGTTTAACCGGAAATACACCGCTTACTGCTGTACAGCTTCTCTGGGTCAACATGATCATGGACACTCTAGGAGCCCTTGCATTAGCCACTGAGCCGCCTAATGATGATTTGATGAAGAGAACACCAGTTGGTAGGACAGGAAACTTCATCACTAATGTGATGTGGAGGAATATCCTCGGGCAGTCCCTGTATCAGTTTGTGATATTATGGTTTCTACAGACCAGAGGAAAAGAAGCTTTCCATGTTGTCGGCCCAGACTCTGATTTGATCTTGAACACCCTTATCTTCAACTCATTTGTGTTTTGTCAGGTTTTCAATGAGATCAGCTCGAGGGAAATGGAGAAGATAAACGTCTTCAAGGGCATAACGCAGAACTACGTTTTCGTGTCTGTCCTCTCTGCCACTGTTATCTTTCAAATCATAATCATCGAGTTCCTGGGTACATTCGCAAGCACATATCCTCTAAGTTTGAAGCAATGGTTTGTTAGCGTTGCGCTTGGGTTCCTTGGCATGCCGGTGTCAGCAGCTCTGAAATTCATCCCCGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005216 GO:0005261 GO:0005262 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0009507 GO:0009526 GO:0009528 GO:0009536 GO:0009706 GO:0009941 GO:0012505 GO:0015075 GO:0015085 GO:0015267 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0019866 GO:0022803 GO:0022804 GO:0022838 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031224 GO:0031226 GO:0031967 GO:0031969 GO:0031975 GO:0034220 GO:0042170 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase Hydrolase_3

Protein Analysis

1017

Amino Acids

110.71

Weight (kDa)

6.31

Isoelectric Point (pI)

37.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CaATP_NAI	PF12515	5 - 49	7.2e-20	Ca2+-ATPase N terminal autoinhibitory domain
Cation_ATPase_N	PF00690	116 - 185	4.6e-12	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	236 - 334	1.5e-20	P-type ATPase actuator domain
Cation_ATPase	PF13246	503 - 662	1.6e-32	P-type ATPase, cytoplasmic domain N
Hydrolase	PF00702	649 - 769	2.4e-10	haloacid dehalogenase-like hydrolase
Cation_ATPase_C	PF00689	840 - 1014	1e-40	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0003293)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G27770 AT1G27770 AT1G27770 AT1G27770
fragaria_vesca	FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180
malus_domestica	MD08G1085200.v1.1 MD08G1085600.v1.1 MD12G1068300.v1.1 MD15G1070800.v1.1
prunus_persica	Prupe.1G423500_v2.0.a1 Prupe.1G423500_v2.0.a1 Prupe.1G423500_v2.0.a1 Prupe.1G423500_v2.0.a1 Prupe.1G423500_v2.0.a1
pyrus_communis	pycom08g07020 pycom15g06650 pycom15g06660
rosa_chinensis	RchiOBHm_Chr6g0309491
rosa_laevigata	RLG00000010561
rosa_multiflora	Rmu_sc0007283.1_g000007
rosa_roxburghii	Rroxscaffold_7G00159360
rosa_rugosa	Rorug06G0372300 Rorug06G0372400
rosa_samantha	Rh6AG485700 Rh6BG495000
rosa_wichuraiana	Rw6G042300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	663
Acc36I	ACCTGC	1 cut(s)	1027
AccB1I	GGYRCC	1 cut(s)	593
AccB7I	CCANNNNNTGG	2 cut(s)	479, 815
AciI	CCGC	8 cut(s)	139, 278, 1132, 1323, 1617, 1932, 2522, 2597
AclI	AACGTT	1 cut(s)	1849
AclWI	GGATC	6 cut(s)	316, 946, 1143, 1156, 1348, 1967
AcoI	YGGCCR	2 cut(s)	584, 1320
AcsI	RAATTY	9 cut(s)	288, 402, 458, 1598, 1775, 2091, 2129, 2441, 3035
AcuI	CTGAAG	4 cut(s)	47, 200, 1734, 2162
AcvI	CACGTG	1 cut(s)	1947
AfaI	GTAC	6 cut(s)	258, 1943, 2275, 2426, 2535, 2944
AfiI	CCNNNNNNNGG	8 cut(s)	479, 815, 1036, 1484, 1718, 1937, 1938, 2461
AflII	CTTAAG	1 cut(s)	232
AflIII	ACRYGT	2 cut(s)	1944, 1946
AgeI	ACCGGT	1 cut(s)	880
AhdI	GACNNNNNGTC	1 cut(s)	1263
AhlI	ACTAGT	2 cut(s)	653, 1306
AjnI	CCWGG	4 cut(s)	769, 1477, 1984, 2937
AleI	CACNNNNGTG	1 cut(s)	2657
AloI	GAACNNNNNNTCC	2 cut(s)	912, 944
Alw21I	GWGCWC	1 cut(s)	1734
Alw26I	GTCTC	5 cut(s)	356, 1325, 1486, 1577, 2043
Alw44I	GTGCAC	1 cut(s)	1730
AlwI	GGATC	6 cut(s)	316, 946, 1143, 1156, 1348, 1967
AlwNI	CAGNNNCTG	3 cut(s)	748, 2689, 2762
Ama87I	CYCGRG	2 cut(s)	2678, 2830
AoxI	GGCC	9 cut(s)	147, 322, 584, 1078, 1320, 1464, 1783, 2122, 2752
ApaI	GGGCCC	1 cut(s)	1787
ApaLI	GTGCAC	1 cut(s)	1730
ApeKI	GCWGC	5 cut(s)	209, 275, 424, 1763, 3026
ApoI	RAATTY	9 cut(s)	288, 402, 458, 1598, 1775, 2091, 2129, 2441, 3035
ArsI	GACNNNNNNTTYG	4 cut(s)	32, 64, 1576, 1608
AsiGI	ACCGGT	1 cut(s)	880
Asp700I	GAANNNNTTC	2 cut(s)	507, 765
AspLEI	GCGC	4 cut(s)	61, 214, 1484, 2998
AspS9I	GGNCC	6 cut(s)	805, 1783, 1784, 2123, 2235, 2753
AsuC2I	CCSGG	1 cut(s)	1727
AsuHPI	GGTGA	4 cut(s)	785, 1631, 2190, 2279
AvaI	CYCGRG	2 cut(s)	2678, 2830
AvaII	GGWCC	2 cut(s)	805, 2235
AxyI	CCTNAGG	3 cut(s)	261, 1936, 2231
BaeGI	GKGCMC	4 cut(s)	547, 598, 1734, 1787
BaeI	ACNNNNGTAYC	2 cut(s)	1933, 1966
BalI	TGGCCA	1 cut(s)	586
BamHI	GGATCC	1 cut(s)	1148
BanI	GGYRCC	1 cut(s)	593
BanII	GRGCYC	3 cut(s)	1668, 1787, 2578
BbrPI	CACGTG	1 cut(s)	1947
BbsI	GAAGAC	1 cut(s)	2848
Bbv12I	GWGCWC	1 cut(s)	1734
BbvI	GCAGC	5 cut(s)	196, 262, 411, 1750, 3038
BceAI	ACGGC	2 cut(s)	309, 2243
BcgI	CGANNNNNNTGC	2 cut(s)	610, 644
BciT130I	CCWGG	4 cut(s)	771, 1479, 1986, 2939
BclI	TGATCA	2 cut(s)	898, 2556
BcnI	CCSGG	1 cut(s)	1727
BcoDI	GTCTC	5 cut(s)	356, 1325, 1486, 1577, 2043
BcuI	ACTAGT	2 cut(s)	653, 1306
BfaI	CTAG	7 cut(s)	654, 726, 1307, 1703, 1857, 2219, 2570
BfmI	CTRYAG	4 cut(s)	674, 1203, 1357, 2717
BfoI	RGCGCY	2 cut(s)	62, 1485
BfrI	CTTAAG	1 cut(s)	232
BfuAI	ACCTGC	1 cut(s)	1027
BglI	GCCNNNNNGGC	1 cut(s)	1791
BglII	AGATCT	1 cut(s)	799
BisI	GCNGC	8 cut(s)	210, 276, 425, 1132, 1323, 1764, 2597, 3027
BlsI	GCNGC	8 cut(s)	211, 277, 426, 1133, 1324, 1765, 2598, 3028
Bme1390I	CCNGG	5 cut(s)	771, 1479, 1727, 1986, 2939
Bme18I	GGWCC	2 cut(s)	805, 2235
BmeRI	GACNNNNNGTC	1 cut(s)	1263
BmeT110I	CYCGRG	2 cut(s)	2678, 2830
BmgT120I	GGNCC	6 cut(s)	805, 1783, 1784, 2123, 2235, 2753
BmiI	GGNNCC	7 cut(s)	595, 807, 1150, 1441, 1785, 2575, 3005
BmrFI	CCNGG	5 cut(s)	771, 1479, 1727, 1986, 2939
BmrI	ACTGGG	3 cut(s)	237, 973, 1380
BmsI	GCATC	6 cut(s)	939, 1071, 1652, 1826, 1848, 2272
BmuI	ACTGGG	3 cut(s)	237, 973, 1380
BpiI	GAAGAC	1 cut(s)	2848
BplI	GAGNNNNNCTC	2 cut(s)	2813, 2845
BpmI	CTGGAG	5 cut(s)	888, 1188, 1545, 2067, 2271
BpuMI	CCSGG	1 cut(s)	1727
Bsa29I	ATCGAT	1 cut(s)	311
BsaAI	YACGTR	1 cut(s)	1947
BsaJI	CCNNGG	6 cut(s)	150, 226, 478, 2677, 2938, 3007
BsaWI	WCCGGW	2 cut(s)	880, 2510
Bsc4I	CCNNNNNNNGG	8 cut(s)	479, 815, 1036, 1484, 1718, 1937, 1938, 2461
Bse118I	RCCGGY	2 cut(s)	880, 3016
Bse21I	CCTNAGG	3 cut(s)	261, 1936, 2231
Bse3DI	GCAATG	4 cut(s)	1909, 2325, 2325, 2984
BseBI	CCWGG	4 cut(s)	771, 1479, 1986, 2939
BseCI	ATCGAT	1 cut(s)	311
BseDI	CCNNGG	6 cut(s)	150, 226, 478, 2677, 2938, 3007
BseGI	GGATG	6 cut(s)	473, 901, 1611, 1817, 2380, 3039
BseLI	CCNNNNNNNGG	8 cut(s)	479, 815, 1036, 1484, 1718, 1937, 1938, 2461
BseMI	GCAATG	4 cut(s)	1909, 2325, 2325, 2984
BseRI	GAGGAG	5 cut(s)	68, 88, 1029, 1542, 2162
BseSI	GKGCMC	4 cut(s)	547, 598, 1734, 1787
BseXI	GCAGC	5 cut(s)	196, 262, 411, 1750, 3038
BseYI	CCCAGC	1 cut(s)	808
BshFI	GGCC	9 cut(s)	149, 324, 586, 1080, 1322, 1466, 1785, 2124, 2754
BshNI	GGYRCC	1 cut(s)	593
BshTI	ACCGGT	1 cut(s)	880
BshVI	ATCGAT	1 cut(s)	311
BsiHKAI	GWGCWC	1 cut(s)	1734
BsiHKCI	CYCGRG	2 cut(s)	2678, 2830
BsiSI	CCGG	4 cut(s)	881, 1726, 2511, 3017
BslFI	GGGAC	4 cut(s)	791, 1387, 2427, 2671
BslI	CCNNNNNNNGG	8 cut(s)	479, 815, 1036, 1484, 1718, 1937, 1938, 2461
BsmAI	GTCTC	5 cut(s)	356, 1325, 1486, 1577, 2043
BsmFI	GGGAC	4 cut(s)	791, 1387, 2427, 2671
BsnI	GGCC	9 cut(s)	149, 324, 586, 1080, 1322, 1466, 1785, 2124, 2754
BsoBI	CYCGRG	2 cut(s)	2678, 2830
Bsp120I	GGGCCC	1 cut(s)	1783
Bsp1286I	GDGCHC	6 cut(s)	547, 598, 1668, 1734, 1787, 2578
Bsp1407I	TGTACA	1 cut(s)	2533
Bsp19I	CCATGG	1 cut(s)	226
BspACI	CCGC	8 cut(s)	139, 278, 1132, 1323, 1617, 1932, 2522, 2597
BspANI	GGCC	9 cut(s)	149, 324, 586, 1080, 1322, 1466, 1785, 2124, 2754
BspDI	ATCGAT	1 cut(s)	311
BspHI	TCATGA	3 cut(s)	172, 525, 2296
BspLI	GGNNCC	7 cut(s)	595, 807, 1150, 1441, 1785, 2575, 3005
BspMAI	CTGCAG	2 cut(s)	678, 1361
BspMI	ACCTGC	1 cut(s)	1027
BspPI	GGATC	6 cut(s)	316, 946, 1143, 1156, 1348, 1967
BspT107I	GGYRCC	1 cut(s)	593
BspTI	CTTAAG	1 cut(s)	232
BsrDI	GCAATG	4 cut(s)	1909, 2325, 2325, 2984
BsrFI	RCCGGY	2 cut(s)	880, 3016
BsrGI	TGTACA	1 cut(s)	2533
BssAI	RCCGGY	2 cut(s)	880, 3016
BssECI	CCNNGG	6 cut(s)	150, 226, 478, 2677, 2938, 3007
BssT1I	CCWWGG	2 cut(s)	226, 3007
Bst2UI	CCWGG	4 cut(s)	771, 1479, 1986, 2939
Bst4CI	ACNGT	8 cut(s)	256, 715, 964, 1399, 2032, 2401, 2464, 2908
Bst6I	CTCTTC	2 cut(s)	2135, 2612
BstAFI	CTTAAG	1 cut(s)	232
BstAPI	GCANNNNNTGC	2 cut(s)	542, 673
BstAUI	TGTACA	1 cut(s)	2533
BstBAI	YACGTR	1 cut(s)	1947
BstC8I	GCNNGC	7 cut(s)	622, 1060, 1136, 1411, 2316, 2953, 3014
BstDSI	CCRYGG	2 cut(s)	226, 478
BstF5I	GGATG	6 cut(s)	473, 901, 1611, 1817, 2380, 3039
BstH2I	RGCGCY	2 cut(s)	62, 1485
BstHHI	GCGC	4 cut(s)	61, 214, 1484, 2998
BstMAI	GTCTC	5 cut(s)	356, 1325, 1486, 1577, 2043
BstMWI	GCNNNNNNNGC	5 cut(s)	542, 673, 1623, 1791, 1862
BstNI	CCWGG	4 cut(s)	771, 1479, 1986, 2939
BstNSI	RCATGY	2 cut(s)	1770, 3016
BstSCI	CCNGG	5 cut(s)	769, 1477, 1725, 1984, 2937
BstSFI	CTRYAG	4 cut(s)	674, 1203, 1357, 2717
BstSLI	GKGCMC	4 cut(s)	547, 598, 1734, 1787
BstV1I	GCAGC	5 cut(s)	196, 262, 411, 1750, 3038
BstV2I	GAAGAC	1 cut(s)	2848
BstX2I	RGATCY	3 cut(s)	799, 1148, 1972
BstYI	RGATCY	3 cut(s)	799, 1148, 1972
Bsu15I	ATCGAT	1 cut(s)	311
Bsu36I	CCTNAGG	3 cut(s)	261, 1936, 2231
BsuRI	GGCC	9 cut(s)	149, 324, 586, 1080, 1322, 1466, 1785, 2124, 2754
BsuTUI	ATCGAT	1 cut(s)	311
BtgI	CCRYGG	2 cut(s)	226, 478
BtsCI	GGATG	6 cut(s)	473, 901, 1611, 1817, 2380, 3039
BtsI	GCAGTG	4 cut(s)	120, 645, 1366, 2061
BveI	ACCTGC	1 cut(s)	1027
Cac8I	GCNNGC	7 cut(s)	622, 1060, 1136, 1411, 2316, 2953, 3014
CaiI	CAGNNNCTG	3 cut(s)	748, 2689, 2762
CciI	TCATGA	3 cut(s)	172, 525, 2296
CfoI	GCGC	4 cut(s)	61, 214, 1484, 2998
Cfr10I	RCCGGY	2 cut(s)	880, 3016
Cfr13I	GGNCC	6 cut(s)	805, 1783, 1784, 2123, 2235, 2753
ClaI	ATCGAT	1 cut(s)	311
CseI	GACGC	2 cut(s)	171, 1184
Csp6I	GTAC	6 cut(s)	257, 1942, 2274, 2425, 2534, 2943
CspAI	ACCGGT	1 cut(s)	880
CviQI	GTAC	6 cut(s)	257, 1942, 2274, 2425, 2534, 2943
DriI	GACNNNNNGTC	1 cut(s)	1263
EaeI	YGGCCR	2 cut(s)	584, 1320
Eam1104I	CTCTTC	2 cut(s)	2135, 2612
Eam1105I	GACNNNNNGTC	1 cut(s)	1263
EarI	CTCTTC	2 cut(s)	2135, 2612
Eco130I	CCWWGG	2 cut(s)	226, 3007
Eco147I	AGGCCT	1 cut(s)	1080
Eco24I	GRGCYC	3 cut(s)	1668, 1787, 2578
Eco47I	GGWCC	2 cut(s)	805, 2235
Eco57I	CTGAAG	4 cut(s)	47, 200, 1734, 2162
Eco72I	CACGTG	1 cut(s)	1947
Eco81I	CCTNAGG	3 cut(s)	261, 1936, 2231
Eco88I	CYCGRG	2 cut(s)	2678, 2830
EcoRII	CCWGG	4 cut(s)	769, 1477, 1984, 2937
EcoT14I	CCWWGG	2 cut(s)	226, 3007
EcoT38I	GRGCYC	3 cut(s)	1668, 1787, 2578
ErhI	CCWWGG	2 cut(s)	226, 3007
FalI	AAGNNNNNCTT	4 cut(s)	198, 230, 2224, 2256
FaqI	GGGAC	4 cut(s)	791, 1387, 2427, 2671
FauI	CCCGC	2 cut(s)	271, 1939
FbaI	TGATCA	2 cut(s)	898, 2556
Fnu4HI	GCNGC	8 cut(s)	210, 276, 425, 1132, 1323, 1764, 2597, 3027
FokI	GGATG	6 cut(s)	460, 888, 1618, 1804, 2387, 3026
FriOI	GRGCYC	3 cut(s)	1668, 1787, 2578
Fsp4HI	GCNGC	8 cut(s)	210, 276, 425, 1132, 1323, 1764, 2597, 3027
FspBI	CTAG	7 cut(s)	654, 726, 1307, 1703, 1857, 2219, 2570
GlaI	GCGC	4 cut(s)	60, 213, 1483, 2997
GluI	GCNGC	8 cut(s)	210, 276, 425, 1132, 1323, 1764, 2597, 3027
GsaI	CCCAGC	1 cut(s)	812
GsuI	CTGGAG	5 cut(s)	888, 1188, 1545, 2067, 2271
HaeII	RGCGCY	2 cut(s)	62, 1485
HaeIII	GGCC	9 cut(s)	149, 324, 586, 1080, 1322, 1466, 1785, 2124, 2754
HapII	CCGG	4 cut(s)	881, 1726, 2511, 3017
HgaI	GACGC	2 cut(s)	171, 1184
HhaI	GCGC	4 cut(s)	61, 214, 1484, 2998
Hin6I	GCGC	4 cut(s)	59, 212, 1482, 2996
HinP1I	GCGC	4 cut(s)	59, 212, 1482, 2996
HincII	GTYRAC	3 cut(s)	2460, 2488, 2551
HindII	GTYRAC	3 cut(s)	2460, 2488, 2551
HindIII	AAGCTT	5 cut(s)	377, 1136, 1407, 1452, 2736
HinfI	GANTC	8 cut(s)	97, 117, 735, 854, 1264, 1494, 1997, 2759
HpaI	GTTAAC	1 cut(s)	2460
HpaII	CCGG	4 cut(s)	881, 1726, 2511, 3017
HphI	GGTGA	4 cut(s)	785, 1631, 2190, 2279
Hpy166II	GTNNAC	8 cut(s)	878, 989, 1732, 1944, 2425, 2460, 2488, 2551
Hpy8I	GTNNAC	8 cut(s)	878, 989, 1732, 1944, 2425, 2460, 2488, 2551
Hpy99I	CGWCG	2 cut(s)	52, 165
HpyCH4III	ACNGT	8 cut(s)	256, 715, 964, 1399, 2032, 2401, 2464, 2908
HpyCH4IV	ACGT	5 cut(s)	1849, 1946, 2416, 2853, 2880
HpyF10VI	GCNNNNNNNGC	5 cut(s)	542, 673, 1623, 1791, 1862
HpySE526I	ACGT	5 cut(s)	1849, 1946, 2416, 2853, 2880
HspAI	GCGC	4 cut(s)	59, 212, 1482, 2996
Ksp22I	TGATCA	2 cut(s)	898, 2556
KspAI	GTTAAC	1 cut(s)	2460
LmnI	GCTCC	3 cut(s)	170, 2290, 2573
Lsp1109I	GCAGC	5 cut(s)	196, 262, 411, 1750, 3038
LweI	GCATC	6 cut(s)	939, 1071, 1652, 1826, 1848, 2272
MaeI	CTAG	7 cut(s)	654, 726, 1307, 1703, 1857, 2219, 2570
MaeII	ACGT	5 cut(s)	1849, 1946, 2416, 2853, 2880
MfeI	CAATTG	4 cut(s)	1215, 2073, 2115, 2391
MflI	RGATCY	3 cut(s)	799, 1148, 1972
MhlI	GDGCHC	6 cut(s)	547, 598, 1668, 1734, 1787, 2578
MlsI	TGGCCA	1 cut(s)	586
MluNI	TGGCCA	1 cut(s)	586
MlyI	GAGTC	4 cut(s)	1273, 1488, 2006, 2753
MmeI	TCCRAC	3 cut(s)	30, 286, 946
Mox20I	TGGCCA	1 cut(s)	586
MroXI	GAANNNNTTC	2 cut(s)	507, 765
MscI	TGGCCA	1 cut(s)	586
MslI	CAYNNNNRTG	5 cut(s)	354, 2558, 2657, 3017, 3044
Msp20I	TGGCCA	1 cut(s)	586
MspA1I	CMGCKG	2 cut(s)	812, 1916
MspCI	CTTAAG	1 cut(s)	232
MspI	CCGG	4 cut(s)	881, 1726, 2511, 3017
MspR9I	CCNGG	5 cut(s)	771, 1479, 1727, 1986, 2939
MunI	CAATTG	4 cut(s)	1215, 2073, 2115, 2391
MvaI	CCWGG	4 cut(s)	771, 1479, 1986, 2939
MwoI	GCNNNNNNNGC	5 cut(s)	542, 673, 1623, 1791, 1862
NciI	CCSGG	1 cut(s)	1727
NcoI	CCATGG	1 cut(s)	226
NlaIV	GGNNCC	7 cut(s)	595, 807, 1150, 1441, 1785, 2575, 3005
NmeAIII	GCCGAG	1 cut(s)	175
NmuCI	GTSAC	8 cut(s)	643, 773, 1096, 1255, 1361, 1769, 2260, 2395
NspI	RCATGY	2 cut(s)	1770, 3016
OliI	CACNNNNGTG	1 cut(s)	2657
PaeI	GCATGC	1 cut(s)	3016
PaeR7I	CTCGAG	1 cut(s)	2830
PagI	TCATGA	3 cut(s)	172, 525, 2296
PceI	AGGCCT	1 cut(s)	1080
PcsI	WCGNNNNNNNCGW	1 cut(s)	24
PdmI	GAANNNNTTC	2 cut(s)	507, 765
PfeI	GAWTC	4 cut(s)	97, 117, 735, 854
PflMI	CCANNNNNTGG	2 cut(s)	479, 815
PinAI	ACCGGT	1 cut(s)	880
PkrI	GCNGC	8 cut(s)	211, 277, 426, 1133, 1324, 1765, 2598, 3028
PleI	GAGTC	4 cut(s)	1272, 1488, 2005, 2753
PmaCI	CACGTG	1 cut(s)	1947
PmlI	CACGTG	1 cut(s)	1947
PpsI	GAGTC	4 cut(s)	1272, 1488, 2005, 2753
Ppu21I	YACGTR	1 cut(s)	1947
PsiI	TTATAA	1 cut(s)	663
Psp1406I	AACGTT	1 cut(s)	1849
Psp6I	CCWGG	4 cut(s)	769, 1477, 1984, 2937
PspCI	CACGTG	1 cut(s)	1947
PspFI	CCCAGC	1 cut(s)	808
PspGI	CCWGG	4 cut(s)	769, 1477, 1984, 2937
PspN4I	GGNNCC	7 cut(s)	595, 807, 1150, 1441, 1785, 2575, 3005
PspOMI	GGGCCC	1 cut(s)	1783
PspPI	GGNCC	6 cut(s)	805, 1783, 1784, 2123, 2235, 2753
PspXI	VCTCGAGB	1 cut(s)	2830
PstI	CTGCAG	2 cut(s)	678, 1361
PstNI	CAGNNNCTG	3 cut(s)	748, 2689, 2762
PsuI	RGATCY	3 cut(s)	799, 1148, 1972
PvuII	CAGCTG	2 cut(s)	812, 1916
RsaI	GTAC	6 cut(s)	258, 1943, 2275, 2426, 2535, 2944
RsaNI	GTAC	6 cut(s)	257, 1942, 2274, 2425, 2534, 2943
RseI	CAYNNNNRTG	5 cut(s)	354, 2558, 2657, 3017, 3044
SatI	GCNGC	8 cut(s)	210, 276, 425, 1132, 1323, 1764, 2597, 3027
Sau96I	GGNCC	6 cut(s)	805, 1783, 1784, 2123, 2235, 2753
SchI	GAGTC	4 cut(s)	1273, 1488, 2006, 2753
ScrFI	CCNGG	5 cut(s)	771, 1479, 1727, 1986, 2939
SduI	GDGCHC	6 cut(s)	547, 598, 1668, 1734, 1787, 2578
SfaNI	GCATC	6 cut(s)	939, 1071, 1652, 1826, 1848, 2272
SfcI	CTRYAG	4 cut(s)	674, 1203, 1357, 2717
Sfr274I	CTCGAG	1 cut(s)	2830
SinI	GGWCC	2 cut(s)	805, 2235
SlaI	CTCGAG	1 cut(s)	2830
SmiMI	CAYNNNNRTG	5 cut(s)	354, 2558, 2657, 3017, 3044
SmlI	CTYRAG	2 cut(s)	232, 2830
SmoI	CTYRAG	2 cut(s)	232, 2830
SpeI	ACTAGT	2 cut(s)	653, 1306
SphI	GCATGC	1 cut(s)	3016
SseBI	AGGCCT	1 cut(s)	1080
SsiI	CCGC	8 cut(s)	139, 278, 1132, 1323, 1617, 1932, 2522, 2597
SspI	AATATT	2 cut(s)	1196, 1758
SspMI	CTAG	7 cut(s)	654, 726, 1307, 1703, 1857, 2219, 2570
StuI	AGGCCT	1 cut(s)	1080
StyD4I	CCNGG	5 cut(s)	769, 1477, 1725, 1984, 2937
StyI	CCWWGG	2 cut(s)	226, 3007
TaaI	ACNGT	8 cut(s)	256, 715, 964, 1399, 2032, 2401, 2464, 2908
TaiI	ACGT	5 cut(s)	1852, 1949, 2419, 2856, 2883
TaqI	TCGA	7 cut(s)	27, 160, 311, 1809, 2191, 2831, 2931
TaqII	GACCGA	1 cut(s)	2114
TatI	WGTACW	2 cut(s)	2424, 2533
TauI	GCSGC	3 cut(s)	1134, 1325, 2599
TfiI	GAWTC	4 cut(s)	97, 117, 735, 854
TseFI	GTSAC	8 cut(s)	643, 773, 1096, 1255, 1361, 1769, 2260, 2395
TseI	GCWGC	5 cut(s)	209, 275, 424, 1763, 3026
Tsp45I	GTSAC	8 cut(s)	643, 773, 1096, 1255, 1361, 1769, 2260, 2395
TspGWI	ACGGA	2 cut(s)	121, 1990
Van91I	CCANNNNNTGG	2 cut(s)	479, 815
Vha464I	CTTAAG	1 cut(s)	232
VneI	GTGCAC	1 cut(s)	1730
VpaK11BI	GGWCC	2 cut(s)	805, 2235
XapI	RAATTY	9 cut(s)	288, 402, 458, 1598, 1775, 2091, 2129, 2441, 3035
XbaI	TCTAGA	1 cut(s)	1702
XceI	RCATGY	2 cut(s)	1770, 3016
XcmI	CCANNNNNNNNNTGG	1 cut(s)	605
XhoI	CTCGAG	1 cut(s)	2830
XmnI	GAANNNNTTC	2 cut(s)	507, 765
XspI	CTAG	7 cut(s)	654, 726, 1307, 1703, 1857, 2219, 2570

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.