Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0003293

Physical Location & Seq

Genomic Coordinates Forward (+)

7104881 .. 7119775

Gene Structure 14895 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1085600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3501 bp

ATGGAGAGCTATTTGAACGAGAACTTTTATGTGAAGGCGAAGAACTCGTCGGAGGAGGCGTTTCAGATATGGAGGAAGCCGTGTGGACTTGTCAAGAATCGGAAACGGAGGTTCAGATTCACTGCTAACCTCCCCAAGCGTTTTGAAGCTGAGGCCATTCGACGAACCAATCAGGGTAAGTTTAGAGTTGCAGTTTTGGATTCACAAGCTGCGCTTCAGTTTATCCAAGGCTTAAGTTTGCCCAGTGATTATACCGTACGTTGGCCAAAGGGTGCCCACGATGGACTTGGAATTGTTATGGCTCGTTCTTCACCAATGGATAAGCATACCCTTGTAAGACACTTAAGGACCACTTTTCAAGAAGTTGTTGCTGTGACTGGTGATGGTACAAACGATGCTCCAGCACTTCATGAAGCTGATATAGGACTTGCAATGGGCACTGCTGGTACTGAGGTGGCAAAGAAAAGTGCTGACGTGATAATTCTGGATGATAACTTTTCTACAATTGTGACTGTGGCCAAATGGGGACGTTCAGTGTACTTAAACATCCAGAAATTTGTTCAGTTCCAGTTAACGGTTAATGTGGTTGCGCTTATTGTCAGCTTCTCTTCCGCCTGTTTAACTGCTGAGGAAGTCAAAGCTGCGGGTTTTCAAATTTGTGCTGATGAGGTGCAATGGATTGTTGAAGGCCGTGATGTGCAATACTTGAAAAGTCATGGTGGTGTGGAGACTATCATAGACAAGCTTGGAACATCCAGTATCAATGGAATTTCTACTTCTGAGAAATTGCTTAATAAGAGAAAAGAAATATATGGGGTTAATAAATTCGTTGAAAAACCATGCAGTGGATTTCTTGTTTATGTGTGGGAAGCCCTTCAGGACACTACCCTCATGATACTTGCATTTTGTGCCTTTGTATCTCTGCTGGTTGGGATAATGACAGAAGGTTGGCCAAAGGGTGCCCATGATGGACTTGGAATTGTTGCTAGCATTTTGCTTGTTGTATTTGTCACTGCAACTAGTGATTATAAACAATCCCTGCAGTTCAAGGATTTGGAGAAGGAGAAGAAAAAAATTACAGTTCAGGTTACTAGAGATGGATTCAGACAGAAGTTGTCCATTTACGATCTACTTCCTGGTGACATTGTTCATCTTTCTATTGGAGATGTGGTCCCAGCTGATGGACTTTTTGTTTCGGAGTTTCCTGTGCTGATAAATGAATCCAGTTTAACAGGAGAGAGTGAACCAGTTAAGGTCAACTCTACGAATCCTTTTCTCCTCTGTGGAACTAAAGTTCAGGATGGATCATGCAAGATGCTTGTAACTACTGTTGGAATGAGAACCCAGTGGGGTAAACTGATGGTTACTCTTAGTGAAGGGGGAGATGATGAAACCCCCTTGCAGGTCAAACTAAATGGTGTGGCAACCATCATTGGGAAAATAGGCCTGTTTTTTGCTGTTGTGACATTTTCTGTATTGGTTCAAGGATTGTTCAGCCGCAATCTTCAACAAGGATCCCACTTGATCTGGTCTGGAGATGAAGCATTGGAAATCTTGGAATATTTTTCTATAGCTGTTACAATTGTTGTTGTTGCTGTTCCAGAGGGTTTGCCTTTGGCTGTGACATTGAGTCTTGCTTTTGCCATGAAGAAGATGATGAACGATAAGGCACTAGTCAGGCATCTGGCTGCTTGTGAGACAATGGGATCAGCCACAACTATCTGCAGTGACAAGACCGGGACACTAACGACGAGCCACATGACTGTTGTGAAAGCTTGCATTTATGGTAAAATCAGAGATGTGGGAACCTCCGAGGAAGCTACTAACTTTGCTTCTATAGTTCCTGAATCTGGTTGGAGGCTTGCGCTGCAATCTTTTTTGAGCATCTTTTGTGGTAAAATCAGAGATGTGGGAACCTCCGAGGAATTTCCTGAATCTGGTTTGAGGCTTATGCTGCAATCTATTTTTAACAACACTGGAGGAGAAGTTGTTAAAAACAAAGATGGCAAGACCGAGTTACTTGGAACACCCACTGAGACTGCTATTTTGGAATTTGGGATGTTGCTAGGCGGTGATTTCAAGGCAGAAAGGGAGGCAACAAAAGTTGTTAAAGTTGAGCCCTTCAATTCTCATGAAAAGCGAATGGGTGTTGTTGTAGAGCTTCCTAAAGGTGGCTTCCGGGTGCACACTAAAGGTGCTTCTGAGATAATATTAGCTGCATGCGACAAAATCATGTGTCCAGATGGCAAGGTTGTTCCCCTCGATAAAGCATCCATTAATTTTTTGAATGGCATCATTGAACGTTTTGCTAGTGAAGCCCTTAGAACTCTCTGCCTTGCATACATGGAGGTCGGCAAATTCTCAGCTGAAAGTCCTATTCCCACCTCAGGGTACACATTTATAGGCATTGTGGGTATCAAAGATCCAGTTCGTCCTGGTGTCAAGGAGTCTGTTGCAATTTGTAGGTCTGCTGGTATTACAGTCAGAATGATTACTGGAGACGACATAAACACTGCAAAAGCAATTGCAAGAGAGTGTGGAATTTTGACCGATGGAGGTTTGGCAATTGAAGGCCCAGTGTTTCGTGAGAAGAGTGAGGAGGAATTACAAAAAATTATTCCAAACCTTCAGGTTATGGCTCGTTCTTCACCAATGGATAAGCATACCCTTGTAAGACACTTAAGGACCACTTTTCAAGAAGTTGTTGCTGTGACTGGTGATGGTACAAACGATGCTCCAGCACTTCATGAAGCTGATATAGGACTTGCAATGGGCACTGCTGGTACTGAGGTGGCAAAGAAAAGTGCTGACGTGATAATTCTGGATGATAACTTTTCTACAATTGTGACTGTGGCCAAATGGGGACGTTCAGTGTACTTGAACATCCAGAAATTTGTTCAGTTCCAGTTAACAGTTAATGTGGTTGCGCTTATTGTCAGCTTCTCTTCCGCCTGTTTAACTGGAAATACTCCCCTTACTGCTGTTCAGCTTCTCTGGGTCAACATGATCATGGACACTCTAGGAGCCCTTGCATTCGCCACCGAGCCACCTAATGACGATTTAATGAAGAGAGCACCGGTTGGCAGGAAAGGAAACTTCATCACTAATGTGATGTGGAGAAATATCCTAGGGCAGTCTCTGTATCAGTTCGTGATAATATGGTTTCTACAGACCAGAGGAAAAGAAGCTTTTCAGCTCGTTGGCCCAGACTCGGATTTGATCTTGACCACCCTTATCTTCAACTCATTTGTGTTTTGTCAGGTTTTCAATGAGATCAGCTCGAGGGAAATGGAGAAGGTTAACGTCTTCAAAGGCATAATGCAGAACTACGTTTTCATGACTGTCCTCTCTGCCACTGTTATCTTTCAAATCATAATCATCGAGTTCTTGGGTACATTTGCGAGCACACATCCTCTAAGTTGGAAGCAATGGTTTGCCAGCGTTGCACTTGGGTTCCTTGGCATGCCGGTTTCAGCAGTTCTGAAATTCATCCCTGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005216 GO:0005261 GO:0005262 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0009507 GO:0009526 GO:0009528 GO:0009536 GO:0009706 GO:0009941 GO:0012505 GO:0015075 GO:0015085 GO:0015267 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0019866 GO:0022803 GO:0022804 GO:0022838 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031224 GO:0031226 GO:0031967 GO:0031969 GO:0031975 GO:0034220 GO:0042170 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase Hydrolase_3

Protein Analysis

1167

Amino Acids

127.46

Weight (kDa)

6.64

Isoelectric Point (pI)

33.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CaATP_NAI	PF12515	5 - 49	2e-17	Ca2+-ATPase N terminal autoinhibitory domain
Cation_ATPase_N	PF00690	238 - 307	9.5e-11	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	358 - 454	1.1e-19	P-type ATPase actuator domain
Cation_ATPase	PF13246	655 - 812	3.2e-33	P-type ATPase, cytoplasmic domain N
Hydrolase	PF00702	799 - 919	9.8e-11	haloacid dehalogenase-like hydrolase
Cation_ATPase_C	PF00689	990 - 1164	9.1e-41	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003293)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G27770 AT1G27770 AT1G27770 AT1G27770
fragaria_vesca	FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180
malus_domestica	MD08G1085200.v1.1 MD08G1085600.v1.1 MD12G1068300.v1.1 MD15G1070800.v1.1
prunus_persica	Prupe.1G423500_v2.0.a1 Prupe.1G423500_v2.0.a1 Prupe.1G423500_v2.0.a1 Prupe.1G423500_v2.0.a1 Prupe.1G423500_v2.0.a1
pyrus_communis	pycom08g07020 pycom15g06650 pycom15g06660
rosa_chinensis	RchiOBHm_Chr6g0309491
rosa_laevigata	RLG00000010561
rosa_multiflora	Rmu_sc0007283.1_g000007
rosa_roxburghii	Rroxscaffold_7G00159360
rosa_rugosa	Rorug06G0372300 Rorug06G0372400
rosa_samantha	Rh6AG485700 Rh6BG495000
rosa_wichuraiana	Rw6G042300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1029
Acc36I	ACCTGC	1 cut(s)	1393
AccB1I	GGYRCC	2 cut(s)	272, 959
AccB7I	CCANNNNNTGG	2 cut(s)	845, 1181
AciI	CCGC	5 cut(s)	612, 644, 1498, 2070, 2949
AclI	AACGTT	1 cut(s)	2302
AclWI	GGATC	5 cut(s)	1312, 1509, 1522, 1714, 2417
AcoI	YGGCCR	4 cut(s)	263, 516, 950, 2853
AcuI	CTGAAG	3 cut(s)	200, 860, 2612
AfaI	GTAC	9 cut(s)	258, 388, 448, 539, 2393, 2725, 2785, 2876, 3394
AflII	CTTAAG	3 cut(s)	232, 343, 2680
AgeI	ACCGGT	1 cut(s)	3076
AhdI	GACNNNNNGTC	1 cut(s)	1629
AhlI	ACTAGT	2 cut(s)	1019, 1672
AjiI	CACGTC	2 cut(s)	475, 2812
AjnI	CCWGG	2 cut(s)	1135, 2434
AleI	CACNNNNGTG	1 cut(s)	3107
AloI	GAACNNNNNNTCC	2 cut(s)	1278, 1310
Alw21I	GWGCWC	3 cut(s)	2187, 3076, 3407
Alw26I	GTCTC	5 cut(s)	722, 1691, 2030, 2493, 3141
Alw44I	GTGCAC	1 cut(s)	2183
AlwI	GGATC	5 cut(s)	1312, 1509, 1522, 1714, 2417
AlwNI	CAGNNNCTG	1 cut(s)	3139
Ama87I	CYCGRG	1 cut(s)	3280
AoxI	GGCC	9 cut(s)	153, 263, 516, 688, 950, 1444, 2572, 2853, 3202
ApaLI	GTGCAC	1 cut(s)	2183
ApeKI	GCWGC	6 cut(s)	209, 641, 1688, 1867, 1954, 2216
ArsI	GACNNNNNNTTYG	4 cut(s)	32, 64, 2029, 2061
AseI	ATTAAT	1 cut(s)	2277
AsiGI	ACCGGT	1 cut(s)	3076
Asp700I	GAANNNNTTC	2 cut(s)	873, 3189
AspA2I	CCTAGG	1 cut(s)	3127
AspLEI	GCGC	4 cut(s)	214, 592, 1867, 2929
AspS9I	GGNCC	5 cut(s)	348, 1171, 2573, 2685, 3203
AsuC2I	CCSGG	2 cut(s)	1738, 2180
AsuHPI	GGTGA	6 cut(s)	303, 392, 1151, 2084, 2640, 2729
AsuNHI	GCTAGC	1 cut(s)	986
AvaI	CYCGRG	1 cut(s)	3280
AvaII	GGWCC	3 cut(s)	348, 1171, 2685
AvrII	CCTAGG	1 cut(s)	3127
AxyI	CCTNAGG	1 cut(s)	2386
BaeGI	GKGCMC	5 cut(s)	277, 440, 964, 2187, 2777
BaeI	ACNNNNGTAYC	4 cut(s)	438, 471, 2775, 2808
BalI	TGGCCA	4 cut(s)	265, 518, 952, 2855
BamHI	GGATCC	1 cut(s)	1514
BanI	GGYRCC	2 cut(s)	272, 959
BanII	GRGCYC	2 cut(s)	2121, 3028
BbsI	GAAGAC	1 cut(s)	3298
Bbv12I	GWGCWC	3 cut(s)	2187, 3076, 3407
BbvCI	CCTCAGC	2 cut(s)	150, 627
BbvI	GCAGC	6 cut(s)	196, 628, 1675, 1854, 1941, 2203
BceAI	ACGGC	2 cut(s)	64, 675
BciT130I	CCWGG	2 cut(s)	1137, 2436
BclI	TGATCA	1 cut(s)	3006
BcnI	CCSGG	2 cut(s)	1738, 2180
BcoDI	GTCTC	5 cut(s)	722, 1691, 2030, 2493, 3141
BcuI	ACTAGT	2 cut(s)	1019, 1672
BfaI	CTAG	8 cut(s)	987, 1020, 1092, 1673, 2066, 2310, 3020, 3128
BfmI	CTRYAG	5 cut(s)	1040, 1569, 1723, 1836, 3167
BfrI	CTTAAG	3 cut(s)	232, 343, 2680
BfuAI	ACCTGC	1 cut(s)	1393
BisI	GCNGC	7 cut(s)	210, 642, 1498, 1689, 1868, 1955, 2217
BlnI	CCTAGG	1 cut(s)	3127
BlsI	GCNGC	7 cut(s)	211, 643, 1499, 1690, 1869, 1956, 2218
Bme1390I	CCNGG	4 cut(s)	1137, 1738, 2180, 2436
Bme18I	GGWCC	3 cut(s)	348, 1171, 2685
BmeRI	GACNNNNNGTC	1 cut(s)	1629
BmeT110I	CYCGRG	1 cut(s)	3280
BmgBI	CACGTC	2 cut(s)	475, 2812
BmgT120I	GGNCC	5 cut(s)	348, 1171, 2573, 2685, 3203
BmiI	GGNNCC	8 cut(s)	274, 961, 1173, 1516, 1807, 1915, 3025, 3455
BmrFI	CCNGG	4 cut(s)	1137, 1738, 2180, 2436
BmrI	ACTGGG	3 cut(s)	237, 1339, 2570
BmsI	GCATC	7 cut(s)	385, 1305, 1690, 1893, 2279, 2301, 2722
BmtI	GCTAGC	1 cut(s)	990
BmuI	ACTGGG	3 cut(s)	237, 1339, 2570
BpiI	GAAGAC	1 cut(s)	3298
BplI	GAGNNNNNCTC	2 cut(s)	3263, 3295
BpmI	CTGGAG	5 cut(s)	384, 1554, 1998, 2517, 2721
Bpu10I	CCTNAGC	2 cut(s)	150, 627
BpuMI	CCSGG	2 cut(s)	1738, 2180
BsaJI	CCNNGG	5 cut(s)	226, 1812, 1920, 3127, 3457
BsaWI	WCCGGW	1 cut(s)	3076
BsaXI	ACNNNNNCTCC	8 cut(s)	44, 74, 1155, 1185, 1971, 1974, 2001, 2004
Bse118I	RCCGGY	2 cut(s)	3076, 3466
Bse21I	CCTNAGG	1 cut(s)	2386
Bse3DI	GCAATG	4 cut(s)	438, 680, 2775, 3434
BseBI	CCWGG	2 cut(s)	1137, 2436
BseDI	CCNNGG	5 cut(s)	226, 1812, 1920, 3127, 3457
BseMI	GCAATG	4 cut(s)	438, 680, 2775, 3434
BseMII	CTCAG	9 cut(s)	141, 441, 618, 771, 2025, 2193, 2376, 2400, 2778
BseRI	GAGGAG	4 cut(s)	68, 1268, 1995, 2612
BseSI	GKGCMC	5 cut(s)	277, 440, 964, 2187, 2777
BseXI	GCAGC	6 cut(s)	196, 628, 1675, 1854, 1941, 2203
BseYI	CCCAGC	1 cut(s)	1174
BshFI	GGCC	9 cut(s)	155, 265, 518, 690, 952, 1446, 2574, 2855, 3204
BshNI	GGYRCC	2 cut(s)	272, 959
BshTI	ACCGGT	1 cut(s)	3076
BsiHKAI	GWGCWC	3 cut(s)	2187, 3076, 3407
BsiHKCI	CYCGRG	1 cut(s)	3280
BsiSI	CCGG	4 cut(s)	1737, 2179, 3077, 3467
BsiWI	CGTACG	1 cut(s)	256
BslFI	GGGAC	4 cut(s)	540, 1157, 1753, 2877
BsmAI	GTCTC	5 cut(s)	722, 1691, 2030, 2493, 3141
BsmBI	CGTCTC	1 cut(s)	2493
BsmFI	GGGAC	4 cut(s)	540, 1157, 1753, 2877
BsmI	GAATGC	1 cut(s)	3032
BsnI	GGCC	9 cut(s)	155, 265, 518, 690, 952, 1446, 2574, 2855, 3204
BsoBI	CYCGRG	1 cut(s)	3280
Bsp1286I	GDGCHC	9 cut(s)	277, 440, 964, 2121, 2187, 2777, 3028, 3076, 3407
Bsp143I	GATC	9 cut(s)	1126, 1304, 1514, 1524, 1706, 2422, 3006, 3219, 3273
BspACI	CCGC	5 cut(s)	612, 644, 1498, 2070, 2949
BspANI	GGCC	9 cut(s)	155, 265, 518, 690, 952, 1446, 2574, 2855, 3204
BspCNI	CTCAG	9 cut(s)	142, 442, 619, 772, 2026, 2194, 2375, 2399, 2779
BspHI	TCATGA	5 cut(s)	409, 891, 2131, 2746, 3336
BspLI	GGNNCC	8 cut(s)	274, 961, 1173, 1516, 1807, 1915, 3025, 3455
BspMAI	CTGCAG	2 cut(s)	1044, 1727
BspMI	ACCTGC	1 cut(s)	1393
BspOI	GCTAGC	1 cut(s)	990
BspPI	GGATC	5 cut(s)	1312, 1509, 1522, 1714, 2417
BspT107I	GGYRCC	2 cut(s)	272, 959
BspTI	CTTAAG	3 cut(s)	232, 343, 2680
BsrDI	GCAATG	4 cut(s)	438, 680, 2775, 3434
BsrFI	RCCGGY	2 cut(s)	3076, 3466
BssAI	RCCGGY	2 cut(s)	3076, 3466
BssECI	CCNNGG	5 cut(s)	226, 1812, 1920, 3127, 3457
BssMI	GATC	9 cut(s)	1126, 1304, 1514, 1524, 1706, 2422, 3006, 3219, 3273
BssT1I	CCWWGG	3 cut(s)	226, 3127, 3457
Bst2UI	CCWGG	2 cut(s)	1137, 2436
Bst6I	CTCTTC	4 cut(s)	613, 2585, 2950, 3062
BstAFI	CTTAAG	3 cut(s)	232, 343, 2680
BstAPI	GCANNNNNTGC	1 cut(s)	908
BstC8I	GCNNGC	7 cut(s)	988, 1777, 1863, 2221, 3403, 3439, 3464
BstHHI	GCGC	4 cut(s)	214, 592, 1867, 2929
BstKTI	GATC	9 cut(s)	1129, 1307, 1517, 1527, 1709, 2425, 3009, 3222, 3276
BstMAI	GTCTC	5 cut(s)	722, 1691, 2030, 2493, 3141
BstMBI	GATC	9 cut(s)	1126, 1304, 1514, 1524, 1706, 2422, 3006, 3219, 3273
BstMWI	GCNNNNNNNGC	5 cut(s)	908, 1867, 1954, 2315, 3443
BstNI	CCWGG	2 cut(s)	1137, 2436
BstNSI	RCATGY	2 cut(s)	2223, 3466
BstSCI	CCNGG	4 cut(s)	1135, 1736, 2178, 2434
BstSFI	CTRYAG	5 cut(s)	1040, 1569, 1723, 1836, 3167
BstSLI	GKGCMC	5 cut(s)	277, 440, 964, 2187, 2777
BstV1I	GCAGC	6 cut(s)	196, 628, 1675, 1854, 1941, 2203
BstV2I	GAAGAC	1 cut(s)	3298
BstX2I	RGATCY	2 cut(s)	1514, 2422
BstYI	RGATCY	2 cut(s)	1514, 2422
Bsu36I	CCTNAGG	1 cut(s)	2386
BsuRI	GGCC	9 cut(s)	155, 265, 518, 690, 952, 1446, 2574, 2855, 3204
BtrI	CACGTC	2 cut(s)	475, 2812
BtsI	GCAGTG	7 cut(s)	120, 438, 850, 1011, 1732, 2511, 2775
BveI	ACCTGC	1 cut(s)	1393
Cac8I	GCNNGC	7 cut(s)	988, 1777, 1863, 2221, 3403, 3439, 3464
CaiI	CAGNNNCTG	1 cut(s)	3139
CciI	TCATGA	5 cut(s)	409, 891, 2131, 2746, 3336
CfoI	GCGC	4 cut(s)	214, 592, 1867, 2929
Cfr10I	RCCGGY	2 cut(s)	3076, 3466
Cfr13I	GGNCC	5 cut(s)	348, 1171, 2573, 2685, 3203
Csp6I	GTAC	9 cut(s)	257, 387, 447, 538, 2392, 2724, 2784, 2875, 3393
CspAI	ACCGGT	1 cut(s)	3076
CviQI	GTAC	9 cut(s)	257, 387, 447, 538, 2392, 2724, 2784, 2875, 3393
DpnI	GATC	9 cut(s)	1128, 1306, 1516, 1526, 1708, 2424, 3008, 3221, 3275
DpnII	GATC	9 cut(s)	1126, 1304, 1514, 1524, 1706, 2422, 3006, 3219, 3273
DriI	GACNNNNNGTC	1 cut(s)	1629
EaeI	YGGCCR	4 cut(s)	263, 516, 950, 2853
Eam1104I	CTCTTC	4 cut(s)	613, 2585, 2950, 3062
Eam1105I	GACNNNNNGTC	1 cut(s)	1629
EarI	CTCTTC	4 cut(s)	613, 2585, 2950, 3062
EciI	GGCGGA	2 cut(s)	601, 2938
Eco130I	CCWWGG	3 cut(s)	226, 3127, 3457
Eco147I	AGGCCT	1 cut(s)	1446
Eco24I	GRGCYC	2 cut(s)	2121, 3028
Eco47I	GGWCC	3 cut(s)	348, 1171, 2685
Eco57I	CTGAAG	3 cut(s)	200, 860, 2612
Eco81I	CCTNAGG	1 cut(s)	2386
Eco88I	CYCGRG	1 cut(s)	3280
EcoRII	CCWGG	2 cut(s)	1135, 2434
EcoT14I	CCWWGG	3 cut(s)	226, 3127, 3457
EcoT38I	GRGCYC	2 cut(s)	2121, 3028
ErhI	CCWWGG	3 cut(s)	226, 3127, 3457
Esp3I	CGTCTC	1 cut(s)	2493
FalI	AAGNNNNNCTT	6 cut(s)	198, 230, 337, 369, 2674, 2706
FaqI	GGGAC	4 cut(s)	540, 1157, 1753, 2877
FauI	CCCGC	1 cut(s)	637
FbaI	TGATCA	1 cut(s)	3006
Fnu4HI	GCNGC	7 cut(s)	210, 642, 1498, 1689, 1868, 1955, 2217
FriOI	GRGCYC	2 cut(s)	2121, 3028
Fsp4HI	GCNGC	7 cut(s)	210, 642, 1498, 1689, 1868, 1955, 2217
FspBI	CTAG	8 cut(s)	987, 1020, 1092, 1673, 2066, 2310, 3020, 3128
GlaI	GCGC	4 cut(s)	213, 591, 1866, 2928
GluI	GCNGC	7 cut(s)	210, 642, 1498, 1689, 1868, 1955, 2217
GsaI	CCCAGC	1 cut(s)	1178
GsuI	CTGGAG	5 cut(s)	384, 1554, 1998, 2517, 2721
HaeIII	GGCC	9 cut(s)	155, 265, 518, 690, 952, 1446, 2574, 2855, 3204
HapII	CCGG	4 cut(s)	1737, 2179, 3077, 3467
HhaI	GCGC	4 cut(s)	214, 592, 1867, 2929
Hin6I	GCGC	4 cut(s)	212, 590, 1865, 2927
HinP1I	GCGC	4 cut(s)	212, 590, 1865, 2927
HincII	GTYRAC	5 cut(s)	573, 1258, 2910, 3001, 3301
HindII	GTYRAC	5 cut(s)	573, 1258, 2910, 3001, 3301
HindIII	AAGCTT	3 cut(s)	743, 1773, 3186
HpaI	GTTAAC	3 cut(s)	573, 2910, 3301
HpaII	CCGG	4 cut(s)	1737, 2179, 3077, 3467
HphI	GGTGA	6 cut(s)	303, 392, 1151, 2084, 2640, 2729
Hpy99I	CGWCG	3 cut(s)	52, 165, 1753
HpyCH4IV	ACGT	8 cut(s)	259, 474, 529, 2302, 2811, 2866, 3303, 3330
HpyF10VI	GCNNNNNNNGC	5 cut(s)	908, 1867, 1954, 2315, 3443
HpySE526I	ACGT	8 cut(s)	259, 474, 529, 2302, 2811, 2866, 3303, 3330
HspAI	GCGC	4 cut(s)	212, 590, 1865, 2927
Ksp22I	TGATCA	1 cut(s)	3006
KspAI	GTTAAC	3 cut(s)	573, 2910, 3301
Kzo9I	GATC	9 cut(s)	1126, 1304, 1514, 1524, 1706, 2422, 3006, 3219, 3273
LmnI	GCTCC	3 cut(s)	403, 2740, 3023
Lsp1109I	GCAGC	6 cut(s)	196, 628, 1675, 1854, 1941, 2203
LweI	GCATC	7 cut(s)	385, 1305, 1690, 1893, 2279, 2301, 2722
MaeI	CTAG	8 cut(s)	987, 1020, 1092, 1673, 2066, 2310, 3020, 3128
MaeII	ACGT	8 cut(s)	259, 474, 529, 2302, 2811, 2866, 3303, 3330
MalI	GATC	9 cut(s)	1128, 1306, 1516, 1526, 1708, 2424, 3008, 3221, 3275
MboI	GATC	9 cut(s)	1126, 1304, 1514, 1524, 1706, 2422, 3006, 3219, 3273
MfeI	CAATTG	5 cut(s)	504, 1581, 2523, 2565, 2841
MflI	RGATCY	2 cut(s)	1514, 2422
MhlI	GDGCHC	9 cut(s)	277, 440, 964, 2121, 2187, 2777, 3028, 3076, 3407
MlsI	TGGCCA	4 cut(s)	265, 518, 952, 2855
MluNI	TGGCCA	4 cut(s)	265, 518, 952, 2855
MlyI	GAGTC	3 cut(s)	1639, 2456, 3203
MmeI	TCCRAC	4 cut(s)	30, 1312, 1835, 3401
Mox20I	TGGCCA	4 cut(s)	265, 518, 952, 2855
MroXI	GAANNNNTTC	2 cut(s)	873, 3189
MscI	TGGCCA	4 cut(s)	265, 518, 952, 2855
MslI	CAYNNNNRTG	4 cut(s)	720, 3008, 3107, 3494
Msp20I	TGGCCA	4 cut(s)	265, 518, 952, 2855
MspA1I	CMGCKG	2 cut(s)	1178, 2366
MspCI	CTTAAG	3 cut(s)	232, 343, 2680
MspI	CCGG	4 cut(s)	1737, 2179, 3077, 3467
MspR9I	CCNGG	4 cut(s)	1137, 1738, 2180, 2436
MunI	CAATTG	5 cut(s)	504, 1581, 2523, 2565, 2841
Mva1269I	GAATGC	1 cut(s)	3032
MvaI	CCWGG	2 cut(s)	1137, 2436
MwoI	GCNNNNNNNGC	5 cut(s)	908, 1867, 1954, 2315, 3443
NciI	CCSGG	2 cut(s)	1738, 2180
NdeII	GATC	9 cut(s)	1126, 1304, 1514, 1524, 1706, 2422, 3006, 3219, 3273
NheI	GCTAGC	1 cut(s)	986
NlaIV	GGNNCC	8 cut(s)	274, 961, 1173, 1516, 1807, 1915, 3025, 3455
NmuCI	GTSAC	9 cut(s)	373, 508, 1009, 1139, 1462, 1621, 1727, 2710, 2845
NspI	RCATGY	2 cut(s)	2223, 3466
OliI	CACNNNNGTG	1 cut(s)	3107
PaeI	GCATGC	2 cut(s)	2223, 3466
PaeR7I	CTCGAG	1 cut(s)	3280
PagI	TCATGA	5 cut(s)	409, 891, 2131, 2746, 3336
PceI	AGGCCT	1 cut(s)	1446
PcsI	WCGNNNNNNNCGW	1 cut(s)	56
PctI	GAATGC	1 cut(s)	3032
PdmI	GAANNNNTTC	2 cut(s)	873, 3189
PfeI	GAWTC	8 cut(s)	97, 117, 200, 1101, 1220, 1267, 1847, 1934
Pfl23II	CGTACG	1 cut(s)	256
PflMI	CCANNNNNTGG	2 cut(s)	845, 1181
PinAI	ACCGGT	1 cut(s)	3076
PkrI	GCNGC	7 cut(s)	211, 643, 1499, 1690, 1869, 1956, 2218
PleI	GAGTC	3 cut(s)	1638, 2455, 3203
PpsI	GAGTC	3 cut(s)	1638, 2455, 3203
PshBI	ATTAAT	1 cut(s)	2277
PsiI	TTATAA	1 cut(s)	1029
Psp1406I	AACGTT	1 cut(s)	2302
Psp6I	CCWGG	2 cut(s)	1135, 2434
PspFI	CCCAGC	1 cut(s)	1174
PspGI	CCWGG	2 cut(s)	1135, 2434
PspLI	CGTACG	1 cut(s)	256
PspN4I	GGNNCC	8 cut(s)	274, 961, 1173, 1516, 1807, 1915, 3025, 3455
PspPI	GGNCC	5 cut(s)	348, 1171, 2573, 2685, 3203
PspXI	VCTCGAGB	1 cut(s)	3280
PstI	CTGCAG	2 cut(s)	1044, 1727
PstNI	CAGNNNCTG	1 cut(s)	3139
PsuI	RGATCY	2 cut(s)	1514, 2422
PvuII	CAGCTG	2 cut(s)	1178, 2366
RsaI	GTAC	9 cut(s)	258, 388, 448, 539, 2393, 2725, 2785, 2876, 3394
RsaNI	GTAC	9 cut(s)	257, 387, 447, 538, 2392, 2724, 2784, 2875, 3393
RseI	CAYNNNNRTG	4 cut(s)	720, 3008, 3107, 3494
SatI	GCNGC	7 cut(s)	210, 642, 1498, 1689, 1868, 1955, 2217
Sau3AI	GATC	9 cut(s)	1126, 1304, 1514, 1524, 1706, 2422, 3006, 3219, 3273
Sau96I	GGNCC	5 cut(s)	348, 1171, 2573, 2685, 3203
SchI	GAGTC	3 cut(s)	1639, 2456, 3203
ScrFI	CCNGG	4 cut(s)	1137, 1738, 2180, 2436
SduI	GDGCHC	9 cut(s)	277, 440, 964, 2121, 2187, 2777, 3028, 3076, 3407
SfaNI	GCATC	7 cut(s)	385, 1305, 1690, 1893, 2279, 2301, 2722
SfcI	CTRYAG	5 cut(s)	1040, 1569, 1723, 1836, 3167
Sfr274I	CTCGAG	1 cut(s)	3280
SinI	GGWCC	3 cut(s)	348, 1171, 2685
SlaI	CTCGAG	1 cut(s)	3280
SmiMI	CAYNNNNRTG	4 cut(s)	720, 3008, 3107, 3494
SmlI	CTYRAG	4 cut(s)	232, 343, 2680, 3280
SmoI	CTYRAG	4 cut(s)	232, 343, 2680, 3280
SpeI	ACTAGT	2 cut(s)	1019, 1672
SphI	GCATGC	2 cut(s)	2223, 3466
SseBI	AGGCCT	1 cut(s)	1446
SsiI	CCGC	5 cut(s)	612, 644, 1498, 2070, 2949
SspI	AATATT	2 cut(s)	1562, 2211
SspMI	CTAG	8 cut(s)	987, 1020, 1092, 1673, 2066, 2310, 3020, 3128
StuI	AGGCCT	1 cut(s)	1446
StyD4I	CCNGG	4 cut(s)	1135, 1736, 2178, 2434
StyI	CCWWGG	3 cut(s)	226, 3127, 3457
TaiI	ACGT	8 cut(s)	262, 477, 532, 2305, 2814, 2869, 3306, 3333
TaqI	TCGA	4 cut(s)	160, 2262, 3281, 3381
TaqII	GACCGA	2 cut(s)	2027, 2564
TatI	WGTACW	2 cut(s)	537, 2874
TauI	GCSGC	1 cut(s)	1500
TfiI	GAWTC	8 cut(s)	97, 117, 200, 1101, 1220, 1267, 1847, 1934
TseFI	GTSAC	9 cut(s)	373, 508, 1009, 1139, 1462, 1621, 1727, 2710, 2845
TseI	GCWGC	6 cut(s)	209, 641, 1688, 1867, 1954, 2216
Tsp45I	GTSAC	9 cut(s)	373, 508, 1009, 1139, 1462, 1621, 1727, 2710, 2845
TspGWI	ACGGA	1 cut(s)	121
Van91I	CCANNNNNTGG	2 cut(s)	845, 1181
Vha464I	CTTAAG	3 cut(s)	232, 343, 2680
VneI	GTGCAC	1 cut(s)	2183
VpaK11BI	GGWCC	3 cut(s)	348, 1171, 2685
VspI	ATTAAT	1 cut(s)	2277
XceI	RCATGY	2 cut(s)	2223, 3466
XcmI	CCANNNNNNNNNTGG	2 cut(s)	284, 971
XhoI	CTCGAG	1 cut(s)	3280
XmaJI	CCTAGG	1 cut(s)	3127
XmnI	GAANNNNTTC	2 cut(s)	873, 3189
XspI	CTAG	8 cut(s)	987, 1020, 1092, 1673, 2066, 2310, 3020, 3128

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.