Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0003293

Physical Location & Seq

Genomic Coordinates Reverse (-)

65802053 .. 65808078

Gene Structure 6026 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G042300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3045 bp

ATGGAGAGCTATTTGAACGAGAACTTTGATTTGAAGGCGAAGAACTCGTCGGATGAGGCGCTCCAGAGATGGAGGAAGCTCTGCTGGGTTGTCAAGAATAAGAAACGGAGGTTTCGATTCACTGCTAATCTCGAGAAGCGTTCCGAGGCCCAGACTCTTCGACGCTCTAATCAGGAGAAGTTTAGACTTGCAATCTTGGTTTCGCAAGCTGCACTTCAGTTCATCCAAGGACTAAAATTTTTCAGTGACTACACTGTACCTGAGGAAGTCAAAGCTGCAGGTTTTCAAATTGGTGCTGATGAGTTGGCATCCATTGTTGAAGGTCGTGATGTGAAGAAGCTCAAAATGCATGATGGTGTTATGGGTCTAGTAGGCAAGCTTGGAACATCAATTGTTGATGGCATTTCAACCTCTGAGCAATTGATCAATGCAAGAAAGGAAATTTATGGAGTTAATAAGTTCACTGAGAGCCCATCTCGTGGATTTTTACTATTTGTATGGGAAGCCCTTCAAGACACCACCCTCATGATACTTGGAGTTTGTGCCTTTGTCTCTCTGCTTGTTGGGATAATGACAGAAGGATGGCCAAAGGGTGCCCATGATGGACTTGGAATTGTTGCGAGCATTTTGCTTGTTGTATTTGTGACTGCTACTAGCGATTATAAGCAGTCCCTGCAGTTCAAGGACTTGGACAAGGAGAAGAAAAAGATTACAGTTCATGTTACTAGAAATGGATTCAGACAGAAGTTGTCAATATATGATTTACTTCCTGGTGACGTTGTTCATCTCAATATTGGAGATCTGGTCCCAGCTGATGGACTTTTCATTTCTGGCTTTTCTATGCTGATAAATGAATCCAGCTTAACTGGTGAGAGTGAACCGGTTAATATTAATGATGTAAATCCTTTTCTCCTCTCTGGAACTAAAGTTCAAGATGGATCCTGCAAGATGCTTGTGACTACTGTTGGAATGAGAACCCAGTGGGGTAAATTGATGGCTACTCTTACTGAAGGTGGAGATGATGAAACCCCCTTGCAGGTGAAACTAAACGGTGTGGCAACTATCATTGGTAAAATAGGCCTGTTTTTTGCAGTAGTGACATTTGCTGTATTAGTTCAAGGATTGTTCAGCCGCAAGCTTCAAGAAGGATTAGTATGGTCCGGAGATGATGCATTGGTAATTTTGGAATTTTTCGCTATAGCTGTTACAATTGTAGTTGTTGCCGTTCCTGAAGGTTTGCCTTTGGCTGTAACATTAAGCCTTGCTTTTGCCATGAAGAAGATGATGAATGATAAGGCACTTGTCCGACATTTGGCTGCTTGTGAGACAATGGGATCTGCCACAACTATCTGTAGTGACAAGACTGGAACACTAACAACTAACCGGATGACGGTTGTAAAAACTTGCATTTGTTTGAAAATCAAAAATGTGGCTAGCTCTGCGAAATCATCTGACCTCTGCTCTGAAATACCTGATGCTACTTTGAAAGTTCTACTTCAGTGCATATTTAACAATACTGGAGGAGACATTGTTAAAAACAAAGATGACAGGATTGAGATACTGGGAACACCCACTGACACTGCTGTTTTGGGATTTGGGATGTCACTAGGTGGAGATTTCAAGGCAGAACGACAAGCATCAAAACTTGTGAAAGTTGAGCCCTTCAATTCTGTGAAGAAGCGGATGGCTGTTGTTCTACAGCTTCCTGAAGGTGGCTTCCGAGTGCATTGTAAAGGTGCTTCTGAGATCATTTTAGCTACATGTGACAAATGGATGGGTCCAAATGGGGAGGTAATTCCCCTTGATATAGCTTCCATCAATCTTTTGAATGGTATCATAGAACAGTTTGCTAGTGAAGCCCTTAGAACTCTATGCCTGGCATACATGGACATTGGAAGTGAGTTCTCTGCTGAGAGTCCTATTCCTGTCAATGGATATACATGTATAGGAATTGTGGGTATTAAAGATCCAGTTCGTCCTGGTGTCAAGGAGTCTGTTGAGATTTGTAGGTCTGCTGGTATTACTGTCCGAATGGTTACCGGTGATAACATAAACACTGCAAAAGCAATTGCAAGAGAATGTGGAATTTTGACTGATGGAGGTTTAGCAATTGAAGGCCCAGAATTTCGTGAAAAGAGTGAGGAGGAATTACAAAAAATTATTCCAAAACTTCAGGTAATGGCTCGATCTTCGCCAATGGATAAGCATACCCTTGTAAAACACTTGAGAACCACTTTTGGAGAAGTTGTTGCAGTGACAGGTGATGGTACAAATGATGCTCCAGCACTTCATGAAGCTGATATAGGACTTGCAATGGGAATTGCTGGAACTGAGGTGGCTAAAGAGAGTGCTGATGTAATAATTCTGGATGACAACTTCTCTACAATTGTGACCGTGGCTAAATGGGGGCGTTCAGTTTACTTGAACATACAGAAATTTGTTCAGTTCCAGCTAACAGTTAATGTGGTTGCTCTTGTAGTCAACTTCTCTTCAGCATGTTTAACTGGAAGCGCTCCCCTTACTGCTGTTCAGCTTCTCTGGGTCAACATGATCATGGACACTCTAGGAGCCCTTGCATTAGCCACTGAGCCCCCTAATGATGATTTGATGAAGAGAACACCAGTTGGCAGGACAGGAAACTTCATTACTAATGTGATGTGGAGAAATATCTTGGGGCAGTCGCTGTATCAGTTTGTGGTAATATGGTATCTGCTGACCAGAGGAAAAGAAGCTTTTCAGCTTGTTGGCCCAGATTCAGATTTGATATTAAACACCCTGATTTTCAACTCATTTGTCTTCTGTCAGGCTTTCAATGAGATCAGTTGTAGAGAAATGGAGAAGATCAATGTCTTCAAAGGCATACTGCAGAATTATGTGTTTGTGACTGTTCTCTTCTGCACTATAATCTTCCAAGTTATAATCATCGAGTTCCTGGGTACATTTGCAAGCACATCTCCTCTCACTTGGCAGCAGTGGTTTGTCAGTGTTACACTTGGGTTCCTGGGCATGCCTATTTCAGCAGCTTTGAAATTTATTCCTGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005216 GO:0005261 GO:0005262 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0009507 GO:0009526 GO:0009528 GO:0009536 GO:0009706 GO:0009941 GO:0012505 GO:0015075 GO:0015085 GO:0015267 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0019866 GO:0022803 GO:0022804 GO:0022838 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031224 GO:0031226 GO:0031967 GO:0031969 GO:0031975 GO:0034220 GO:0042170 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase Hydrolase_3

Protein Analysis

1014

Amino Acids

110.72

Weight (kDa)

6.26

Isoelectric Point (pI)

37.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CaATP_NAI	PF12515	5 - 49	1.5e-20	Ca2+-ATPase N terminal autoinhibitory domain
Cation_ATPase_N	PF00690	119 - 185	3.9e-09	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	236 - 334	8.7e-20	P-type ATPase actuator domain
Cation_ATPase	PF13246	501 - 660	4.3e-30	P-type ATPase, cytoplasmic domain N
Hydrolase	PF00702	633 - 767	6.1e-12	haloacid dehalogenase-like hydrolase
Cation_ATPase_C	PF00689	838 - 1012	1.5e-41	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0003293)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G27770 AT1G27770 AT1G27770 AT1G27770
fragaria_vesca	FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180
malus_domestica	MD08G1085200.v1.1 MD08G1085600.v1.1 MD12G1068300.v1.1 MD15G1070800.v1.1
prunus_persica	Prupe.1G423500_v2.0.a1 Prupe.1G423500_v2.0.a1 Prupe.1G423500_v2.0.a1 Prupe.1G423500_v2.0.a1 Prupe.1G423500_v2.0.a1
pyrus_communis	pycom08g07020 pycom15g06650 pycom15g06660
rosa_chinensis	RchiOBHm_Chr6g0309491
rosa_laevigata	RLG00000010561
rosa_multiflora	Rmu_sc0007283.1_g000007
rosa_roxburghii	Rroxscaffold_7G00159360
rosa_rugosa	Rorug06G0372300 Rorug06G0372400
rosa_samantha	Rh6AG485700 Rh6BG495000
rosa_wichuraiana	Rw6G042300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	663, 2918
AarI	CACCTGC	1 cut(s)	1027
Acc36I	ACCTGC	2 cut(s)	269, 1027
AccB1I	GGYRCC	1 cut(s)	593
AccB7I	CCANNNNNTGG	2 cut(s)	479, 815
AccIII	TCCGGA	1 cut(s)	1160
AciI	CCGC	2 cut(s)	1132, 1681
AclWI	GGATC	4 cut(s)	933, 946, 1342, 1961
AcoI	YGGCCR	1 cut(s)	584
AcsI	RAATTY	7 cut(s)	236, 441, 1187, 2085, 2123, 2435, 3029
AcuI	CTGAAG	7 cut(s)	200, 1029, 1251, 1481, 1728, 2156, 2475
AdeI	CACNNNGTG	1 cut(s)	1610
AfaI	GTAC	3 cut(s)	258, 2269, 2938
AfeI	AGCGCT	1 cut(s)	2512
AfiI	CCNNNNNNNGG	7 cut(s)	479, 815, 1036, 1312, 1390, 1712, 1931
AflIII	ACRYGT	2 cut(s)	1760, 1940
AgeI	ACCGGT	2 cut(s)	880, 2039
AjnI	CCWGG	5 cut(s)	769, 1875, 1978, 2931, 3000
AloI	GAACNNNNNNTCC	2 cut(s)	912, 944
Alw26I	GTCTC	3 cut(s)	556, 1319, 1518
AlwI	GGATC	4 cut(s)	933, 946, 1342, 1961
AlwNI	CAGNNNCTG	2 cut(s)	673, 2683
Ama87I	CYCGRG	1 cut(s)	131
Aor13HI	TCCGGA	1 cut(s)	1160
Aor51HI	AGCGCT	1 cut(s)	2512
AoxI	GGCC	5 cut(s)	147, 584, 1078, 2116, 2746
ApeKI	GCWGC	5 cut(s)	209, 275, 1316, 2968, 3020
ApoI	RAATTY	7 cut(s)	236, 441, 1187, 2085, 2123, 2435, 3029
ArsI	GACNNNNNNTTYG	2 cut(s)	32, 64
AseI	ATTAAT	1 cut(s)	891
AsiGI	ACCGGT	2 cut(s)	880, 2039
Asp700I	GAANNNNTTC	2 cut(s)	507, 2733
AspLEI	GCGC	2 cut(s)	61, 2513
AspS9I	GGNCC	6 cut(s)	148, 805, 1158, 1778, 2117, 2747
AsuHPI	GGTGA	5 cut(s)	785, 881, 1051, 2054, 2273
AsuNHI	GCTAGC	1 cut(s)	1433
AvaI	CYCGRG	1 cut(s)	131
AvaII	GGWCC	3 cut(s)	805, 1158, 1778
AxyI	CCTNAGG	1 cut(s)	261
BaeGI	GKGCMC	1 cut(s)	598
BalI	TGGCCA	1 cut(s)	586
BamHI	GGATCC	1 cut(s)	938
BanI	GGYRCC	1 cut(s)	593
BanII	GRGCYC	4 cut(s)	473, 1662, 2572, 2592
BarI	GAAGNNNNNNTAC	2 cut(s)	1723, 1755
BauI	CACGAG	1 cut(s)	477
BbsI	GAAGAC	2 cut(s)	2788, 2842
BbvI	GCAGC	5 cut(s)	196, 262, 1303, 2980, 3032
BceAI	ACGGC	1 cut(s)	1208
BcgI	CGANNNNNNTGC	2 cut(s)	610, 644
BciT130I	CCWGG	5 cut(s)	771, 1877, 1980, 2933, 3002
BclI	TGATCA	2 cut(s)	423, 2550
BcoDI	GTCTC	3 cut(s)	556, 1319, 1518
BfaI	CTAG	7 cut(s)	368, 654, 726, 1434, 1607, 1851, 2564
BfmI	CTRYAG	6 cut(s)	276, 674, 1197, 1351, 1697, 2864
BfoI	RGCGCY	2 cut(s)	62, 2514
BfuAI	ACCTGC	2 cut(s)	269, 1027
BglII	AGATCT	1 cut(s)	799
BisI	GCNGC	6 cut(s)	210, 276, 1132, 1317, 2969, 3021
BlsI	GCNGC	6 cut(s)	211, 277, 1133, 1318, 2970, 3022
Bme1390I	CCNGG	5 cut(s)	771, 1877, 1980, 2933, 3002
Bme18I	GGWCC	3 cut(s)	805, 1158, 1778
BmeT110I	CYCGRG	1 cut(s)	131
BmgT120I	GGNCC	6 cut(s)	148, 805, 1158, 1778, 2117, 2747
BmiI	GGNNCC	6 cut(s)	595, 807, 940, 1779, 2569, 2999
BmrFI	CCNGG	5 cut(s)	771, 1877, 1980, 2933, 3002
BmrI	ACTGGG	2 cut(s)	973, 1571
BmsI	GCATC	6 cut(s)	317, 939, 1159, 1465, 1646, 2266
BmtI	GCTAGC	1 cut(s)	1437
BmuI	ACTGGG	2 cut(s)	973, 1571
BpiI	GAAGAC	2 cut(s)	2788, 2842
BplI	GAGNNNNNCTC	2 cut(s)	460, 492
BpmI	CTGGAG	3 cut(s)	47, 1539, 2265
BpuEI	CTTGAG	1 cut(s)	2245
BsaBI	GATNNNNATC	1 cut(s)	900
BsaJI	CCNNGG	5 cut(s)	144, 226, 2394, 2932, 3001
BsaWI	WCCGGW	4 cut(s)	880, 1160, 1383, 2039
BsaXI	ACNNNNNCTCC	6 cut(s)	789, 819, 1515, 1545, 2232, 2262
Bsc4I	CCNNNNNNNGG	7 cut(s)	479, 815, 1036, 1312, 1390, 1712, 1931
Bse118I	RCCGGY	2 cut(s)	880, 2039
Bse1I	ACTGG	8 cut(s)	871, 979, 1369, 1522, 1566, 1970, 2509, 2621
Bse21I	CCTNAGG	1 cut(s)	261
Bse3DI	GCAATG	1 cut(s)	2319
Bse8I	GATNNNNATC	1 cut(s)	900
BseAI	TCCGGA	1 cut(s)	1160
BseBI	CCWGG	5 cut(s)	771, 1877, 1980, 2933, 3002
BseDI	CCNNGG	5 cut(s)	144, 226, 2394, 2932, 3001
BseGI	GGATG	9 cut(s)	58, 222, 308, 587, 1392, 1605, 1689, 1779, 2374
BseJI	GATNNNNATC	1 cut(s)	900
BseLI	CCNNNNNNNGG	7 cut(s)	479, 815, 1036, 1312, 1390, 1712, 1931
BseMI	GCAATG	1 cut(s)	2319
BseMII	CTCAG	7 cut(s)	252, 405, 456, 1734, 1902, 2322, 2577
BseNI	ACTGG	8 cut(s)	871, 979, 1369, 1522, 1566, 1970, 2509, 2621
BseRI	GAGGAG	4 cut(s)	902, 1536, 2156, 2946
BseSI	GKGCMC	1 cut(s)	598
BseXI	GCAGC	5 cut(s)	196, 262, 1303, 2980, 3032
BseYI	CCCAGC	2 cut(s)	84, 808
BsgI	GTGCAG	2 cut(s)	195, 2881
BshFI	GGCC	5 cut(s)	149, 586, 1080, 2118, 2748
BshNI	GGYRCC	1 cut(s)	593
BshTI	ACCGGT	2 cut(s)	880, 2039
BsiHKCI	CYCGRG	1 cut(s)	131
BsiSI	CCGG	4 cut(s)	881, 1161, 1384, 2040
BslFI	GGGAC	2 cut(s)	655, 791
BslI	CCNNNNNNNGG	7 cut(s)	479, 815, 1036, 1312, 1390, 1712, 1931
BsmAI	GTCTC	3 cut(s)	556, 1319, 1518
BsmFI	GGGAC	2 cut(s)	655, 791
BsnI	GGCC	5 cut(s)	149, 586, 1080, 2118, 2748
BsoBI	CYCGRG	1 cut(s)	131
Bsp1286I	GDGCHC	5 cut(s)	473, 598, 1662, 2572, 2592
Bsp13I	TCCGGA	1 cut(s)	1160
BspACI	CCGC	2 cut(s)	1132, 1681
BspANI	GGCC	5 cut(s)	149, 586, 1080, 2118, 2748
BspCNI	CTCAG	7 cut(s)	253, 406, 457, 1735, 1903, 2323, 2578
BspEI	TCCGGA	1 cut(s)	1160
BspHI	TCATGA	2 cut(s)	525, 2290
BspLI	GGNNCC	6 cut(s)	595, 807, 940, 1779, 2569, 2999
BspMAI	CTGCAG	3 cut(s)	280, 678, 2868
BspMI	ACCTGC	2 cut(s)	269, 1027
BspOI	GCTAGC	1 cut(s)	1437
BspPI	GGATC	4 cut(s)	933, 946, 1342, 1961
BspT107I	GGYRCC	1 cut(s)	593
BsrDI	GCAATG	1 cut(s)	2319
BsrFI	RCCGGY	2 cut(s)	880, 2039
BsrI	ACTGG	8 cut(s)	871, 979, 1369, 1522, 1566, 1970, 2509, 2621
BssAI	RCCGGY	2 cut(s)	880, 2039
BssECI	CCNNGG	5 cut(s)	144, 226, 2394, 2932, 3001
BssSI	CACGAG	1 cut(s)	477
BssT1I	CCWWGG	1 cut(s)	226
Bst2BI	CACGAG	1 cut(s)	477
Bst2UI	CCWGG	5 cut(s)	771, 1877, 1980, 2933, 3002
Bst6I	CTCTTC	4 cut(s)	162, 2494, 2606, 2897
BstAPI	GCANNNNNTGC	1 cut(s)	673
BstC8I	GCNNGC	7 cut(s)	207, 377, 622, 1136, 1435, 2947, 3008
BstDEI	CTNAG	8 cut(s)	261, 414, 465, 1743, 1862, 1911, 2331, 2586
BstDSI	CCRYGG	1 cut(s)	2394
BstEII	GGTNACC	1 cut(s)	2035
BstF5I	GGATG	9 cut(s)	58, 222, 308, 587, 1392, 1605, 1689, 1779, 2374
BstH2I	RGCGCY	2 cut(s)	62, 2514
BstHHI	GCGC	2 cut(s)	61, 2513
BstMAI	GTCTC	3 cut(s)	556, 1319, 1518
BstMWI	GCNNNNNNNGC	4 cut(s)	346, 673, 1439, 1856
BstNI	CCWGG	5 cut(s)	771, 1877, 1980, 2933, 3002
BstNSI	RCATGY	4 cut(s)	1764, 1944, 2499, 3010
BstPI	GGTNACC	1 cut(s)	2035
BstSCI	CCNGG	5 cut(s)	769, 1875, 1978, 2931, 3000
BstSFI	CTRYAG	6 cut(s)	276, 674, 1197, 1351, 1697, 2864
BstSLI	GKGCMC	1 cut(s)	598
BstV1I	GCAGC	5 cut(s)	196, 262, 1303, 2980, 3032
BstV2I	GAAGAC	2 cut(s)	2788, 2842
BstX2I	RGATCY	4 cut(s)	799, 938, 1334, 1966
BstYI	RGATCY	4 cut(s)	799, 938, 1334, 1966
Bsu36I	CCTNAGG	1 cut(s)	261
BsuRI	GGCC	5 cut(s)	149, 586, 1080, 2118, 2748
BtgI	CCRYGG	1 cut(s)	2394
BtsCI	GGATG	9 cut(s)	58, 222, 308, 587, 1392, 1605, 1689, 1779, 2374
BtsI	GCAGTG	5 cut(s)	120, 1578, 2055, 2259, 2978
BveI	ACCTGC	2 cut(s)	269, 1027
Cac8I	GCNNGC	7 cut(s)	207, 377, 622, 1136, 1435, 2947, 3008
CaiI	CAGNNNCTG	2 cut(s)	673, 2683
CciI	TCATGA	2 cut(s)	525, 2290
CfoI	GCGC	2 cut(s)	61, 2513
Cfr10I	RCCGGY	2 cut(s)	880, 2039
Cfr13I	GGNCC	6 cut(s)	148, 805, 1158, 1778, 2117, 2747
CseI	GACGC	1 cut(s)	171
Csp6I	GTAC	3 cut(s)	257, 2268, 2937
CspAI	ACCGGT	2 cut(s)	880, 2039
CviQI	GTAC	3 cut(s)	257, 2268, 2937
DdeI	CTNAG	8 cut(s)	261, 414, 465, 1743, 1862, 1911, 2331, 2586
DraIII	CACNNNGTG	1 cut(s)	1610
EaeI	YGGCCR	1 cut(s)	584
Eam1104I	CTCTTC	4 cut(s)	162, 2494, 2606, 2897
EarI	CTCTTC	4 cut(s)	162, 2494, 2606, 2897
Eco130I	CCWWGG	1 cut(s)	226
Eco147I	AGGCCT	1 cut(s)	1080
Eco24I	GRGCYC	4 cut(s)	473, 1662, 2572, 2592
Eco47I	GGWCC	3 cut(s)	805, 1158, 1778
Eco47III	AGCGCT	1 cut(s)	2512
Eco57I	CTGAAG	7 cut(s)	200, 1029, 1251, 1481, 1728, 2156, 2475
Eco81I	CCTNAGG	1 cut(s)	261
Eco88I	CYCGRG	1 cut(s)	131
Eco91I	GGTNACC	1 cut(s)	2035
EcoO65I	GGTNACC	1 cut(s)	2035
EcoRII	CCWGG	5 cut(s)	769, 1875, 1978, 2931, 3000
EcoT14I	CCWWGG	1 cut(s)	226
EcoT22I	ATGCAT	2 cut(s)	351, 1174
EcoT38I	GRGCYC	4 cut(s)	473, 1662, 2572, 2592
ErhI	CCWWGG	1 cut(s)	226
FalI	AAGNNNNNCTT	6 cut(s)	198, 230, 1249, 1281, 1479, 1511
FaqI	GGGAC	2 cut(s)	655, 791
FbaI	TGATCA	2 cut(s)	423, 2550
Fnu4HI	GCNGC	6 cut(s)	210, 276, 1132, 1317, 2969, 3021
FokI	GGATG	9 cut(s)	65, 209, 295, 594, 1399, 1612, 1696, 1786, 2381
FriOI	GRGCYC	4 cut(s)	473, 1662, 2572, 2592
Fsp4HI	GCNGC	6 cut(s)	210, 276, 1132, 1317, 2969, 3021
FspBI	CTAG	7 cut(s)	368, 654, 726, 1434, 1607, 1851, 2564
GlaI	GCGC	2 cut(s)	60, 2512
GluI	GCNGC	6 cut(s)	210, 276, 1132, 1317, 2969, 3021
GsaI	CCCAGC	2 cut(s)	88, 812
GsuI	CTGGAG	3 cut(s)	47, 1539, 2265
HaeII	RGCGCY	2 cut(s)	62, 2514
HaeIII	GGCC	5 cut(s)	149, 586, 1080, 2118, 2748
HapII	CCGG	4 cut(s)	881, 1161, 1384, 2040
HgaI	GACGC	1 cut(s)	171
HhaI	GCGC	2 cut(s)	61, 2513
Hin6I	GCGC	2 cut(s)	59, 2511
HinP1I	GCGC	2 cut(s)	59, 2511
HincII	GTYRAC	2 cut(s)	2482, 2545
HindII	GTYRAC	2 cut(s)	2482, 2545
HindIII	AAGCTT	3 cut(s)	377, 1136, 2730
HinfI	GANTC	7 cut(s)	117, 154, 735, 854, 1915, 1991, 2753
HpaII	CCGG	4 cut(s)	881, 1161, 1384, 2040
HphI	GGTGA	5 cut(s)	785, 881, 1051, 2054, 2273
Hpy166II	GTNNAC	5 cut(s)	462, 878, 2419, 2482, 2545
Hpy8I	GTNNAC	5 cut(s)	462, 878, 2419, 2482, 2545
Hpy99I	CGWCG	2 cut(s)	52, 165
HpyCH4IV	ACGT	1 cut(s)	777
HpyF10VI	GCNNNNNNNGC	4 cut(s)	346, 673, 1439, 1856
HpyF3I	CTNAG	8 cut(s)	261, 414, 465, 1743, 1862, 1911, 2331, 2586
HpySE526I	ACGT	1 cut(s)	777
HspAI	GCGC	2 cut(s)	59, 2511
Kpn2I	TCCGGA	1 cut(s)	1160
Ksp22I	TGATCA	2 cut(s)	423, 2550
LmnI	GCTCC	4 cut(s)	66, 2284, 2518, 2567
Lsp1109I	GCAGC	5 cut(s)	196, 262, 1303, 2980, 3032
LweI	GCATC	6 cut(s)	317, 939, 1159, 1465, 1646, 2266
MaeI	CTAG	7 cut(s)	368, 654, 726, 1434, 1607, 1851, 2564
MaeII	ACGT	1 cut(s)	777
MfeI	CAATTG	6 cut(s)	390, 419, 1209, 2067, 2109, 2385
MflI	RGATCY	4 cut(s)	799, 938, 1334, 1966
MhlI	GDGCHC	5 cut(s)	473, 598, 1662, 2572, 2592
MlsI	TGGCCA	1 cut(s)	586
MluNI	TGGCCA	1 cut(s)	586
MlyI	GAGTC	3 cut(s)	148, 1924, 2000
MmeI	TCCRAC	3 cut(s)	30, 946, 1330
Mox20I	TGGCCA	1 cut(s)	586
Mph1103I	ATGCAT	2 cut(s)	351, 1174
MroI	TCCGGA	1 cut(s)	1160
MroXI	GAANNNNTTC	2 cut(s)	507, 2733
MscI	TGGCCA	1 cut(s)	586
MslI	CAYNNNNRTG	3 cut(s)	354, 524, 2552
Msp20I	TGGCCA	1 cut(s)	586
MspA1I	CMGCKG	1 cut(s)	812
MspI	CCGG	4 cut(s)	881, 1161, 1384, 2040
MspR9I	CCNGG	5 cut(s)	771, 1877, 1980, 2933, 3002
MunI	CAATTG	6 cut(s)	390, 419, 1209, 2067, 2109, 2385
MvaI	CCWGG	5 cut(s)	771, 1877, 1980, 2933, 3002
MwoI	GCNNNNNNNGC	4 cut(s)	346, 673, 1439, 1856
NheI	GCTAGC	1 cut(s)	1433
NlaIV	GGNNCC	6 cut(s)	595, 807, 940, 1779, 2569, 2999
NsiI	ATGCAT	2 cut(s)	351, 1174
NspI	RCATGY	4 cut(s)	1764, 1944, 2499, 3010
PaeI	GCATGC	1 cut(s)	3010
PaeR7I	CTCGAG	1 cut(s)	131
PagI	TCATGA	2 cut(s)	525, 2290
PaqCI	CACCTGC	1 cut(s)	1027
PceI	AGGCCT	1 cut(s)	1080
PciI	ACATGT	2 cut(s)	1760, 1940
PcsI	WCGNNNNNNNCGW	1 cut(s)	112
PdmI	GAANNNNTTC	2 cut(s)	507, 2733
PfeI	GAWTC	4 cut(s)	117, 735, 854, 2753
PflMI	CCANNNNNTGG	2 cut(s)	479, 815
PinAI	ACCGGT	2 cut(s)	880, 2039
PkrI	GCNGC	6 cut(s)	211, 277, 1133, 1318, 2970, 3022
PleI	GAGTC	3 cut(s)	148, 1923, 1999
PpsI	GAGTC	3 cut(s)	148, 1923, 1999
PscI	ACATGT	2 cut(s)	1760, 1940
PshBI	ATTAAT	1 cut(s)	891
PsiI	TTATAA	2 cut(s)	663, 2918
Psp6I	CCWGG	5 cut(s)	769, 1875, 1978, 2931, 3000
PspEI	GGTNACC	1 cut(s)	2035
PspFI	CCCAGC	2 cut(s)	84, 808
PspGI	CCWGG	5 cut(s)	769, 1875, 1978, 2931, 3000
PspN4I	GGNNCC	6 cut(s)	595, 807, 940, 1779, 2569, 2999
PspPI	GGNCC	6 cut(s)	148, 805, 1158, 1778, 2117, 2747
PstI	CTGCAG	3 cut(s)	280, 678, 2868
PstNI	CAGNNNCTG	2 cut(s)	673, 2683
PsuI	RGATCY	4 cut(s)	799, 938, 1334, 1966
PvuII	CAGCTG	1 cut(s)	812
RsaI	GTAC	3 cut(s)	258, 2269, 2938
RsaNI	GTAC	3 cut(s)	257, 2268, 2937
RseI	CAYNNNNRTG	3 cut(s)	354, 524, 2552
SatI	GCNGC	6 cut(s)	210, 276, 1132, 1317, 2969, 3021
Sau96I	GGNCC	6 cut(s)	148, 805, 1158, 1778, 2117, 2747
SchI	GAGTC	3 cut(s)	148, 1924, 2000
ScrFI	CCNGG	5 cut(s)	771, 1877, 1980, 2933, 3002
SduI	GDGCHC	5 cut(s)	473, 598, 1662, 2572, 2592
SfaNI	GCATC	6 cut(s)	317, 939, 1159, 1465, 1646, 2266
SfcI	CTRYAG	6 cut(s)	276, 674, 1197, 1351, 1697, 2864
Sfr274I	CTCGAG	1 cut(s)	131
SinI	GGWCC	3 cut(s)	805, 1158, 1778
SlaI	CTCGAG	1 cut(s)	131
SmiMI	CAYNNNNRTG	3 cut(s)	354, 524, 2552
SmlI	CTYRAG	2 cut(s)	131, 2224
SmoI	CTYRAG	2 cut(s)	131, 2224
SphI	GCATGC	1 cut(s)	3010
SseBI	AGGCCT	1 cut(s)	1080
SsiI	CCGC	2 cut(s)	1132, 1681
SspI	AATATT	2 cut(s)	793, 889
SspMI	CTAG	7 cut(s)	368, 654, 726, 1434, 1607, 1851, 2564
StuI	AGGCCT	1 cut(s)	1080
StyD4I	CCNGG	5 cut(s)	769, 1875, 1978, 2931, 3000
StyI	CCWWGG	1 cut(s)	226
TaiI	ACGT	1 cut(s)	780
TaqI	TCGA	5 cut(s)	115, 132, 160, 2185, 2925
TauI	GCSGC	1 cut(s)	1134
TfiI	GAWTC	4 cut(s)	117, 735, 854, 2753
TseI	GCWGC	5 cut(s)	209, 275, 1316, 2968, 3020
TspGWI	ACGGA	1 cut(s)	121
Van91I	CCANNNNNTGG	2 cut(s)	479, 815
VpaK11BI	GGWCC	3 cut(s)	805, 1158, 1778
VspI	ATTAAT	1 cut(s)	891
XapI	RAATTY	7 cut(s)	236, 441, 1187, 2085, 2123, 2435, 3029
XceI	RCATGY	4 cut(s)	1764, 1944, 2499, 3010
XcmI	CCANNNNNNNNNTGG	1 cut(s)	605
XhoI	CTCGAG	1 cut(s)	131
XmnI	GAANNNNTTC	2 cut(s)	507, 2733
XspI	CTAG	7 cut(s)	368, 654, 726, 1434, 1607, 1851, 2564
Zsp2I	ATGCAT	2 cut(s)	351, 1174

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.