Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0003293

Physical Location & Seq

Genomic Coordinates Forward (+)

7070999 .. 7077058

Gene Structure 6060 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1085200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3051 bp

ATGGAGAGCTATTTGAACGAGAACTTTGATGTGAAGGCGAAGAACTCGTCGGAGGAGGCGCTTCAGAGATGGAGGAAGCTCTGTGGATTTGTCAAGAATCGGAAACGGAGGTTCAGATTCACTGCTAATCTCCCCAAGCGTTTTGAAGCTGAGGCCATTCGACGATCCAATCAGGAGAAGTTTAGAGTTGCAGTTTTGGTTTCACAAGCTGCGCTTCAGTTTATCCAAGGTTTAAGTTTGCCCAGTGATTATACCGTGCCTGAGGAAGTCAAAGCTGCGGGTTTTCAAATTTGTGCTGATGAGGTGGGATCAATTGTTGATGGCCGTGATTTGAAAAAGCTGAAAAGTCATGGTGGTGTGGAGACTATCACAGACAAGCTTGGAACATCCAGTATCAATGGAATTTTTACTTCTGAGAAATTGCTTAATAAGAGAAAAGAAATATATGGGGTTAATAAATTCATTGAAAAACCGCCCCGTGGATTTCTTGTTTATGTGTGGGAAGCCCTTCAGGACACTACCCTCATGATACTTGCATTTTGTGCCTTTGTATCTCTGCTGGTTGGGATAATGACAGAAGGTTGGCCAAAGGGTGCCCACGATGGACTTGGAATTGTTGCTAGCATTTTGCTTGTTGTATTTGTCACTGCAACTAGTGATTATAAACAATCCCTGCAGTTCAAGGATTTGGAGAAGGAGAAGAAAAAAATTACAGTTCAGGTTACTAGAGATGGATTCAGACAGAAGTTGTCCATTTACGATCTACTTCCTGGCGACCTTGTTCATCTTTCTATTGGAGATCTGGTCCCAGCTGATGGACTTTTTGTTTCGGGGTTTTCTGTGCTGATAAATGAATCCAGTTTAACAGGAGAAAGTGAACCAGTTAATGTCAACTCTACGAATCCTTTTCTCCTCTCTGGAACTAAAGTTCAGGATGGATCATGCAAGATGCTTGTAACTACCGTTGGAATGAGAACCCAGTGGGGTAAACTGATGGCTACTCTTAGTGAAGGAGGAGATGATGAAACCCCCTTGCAGGTCAAACTAAATGGTGTGGCAACCATCATTGGGAAAATAGGCCTGTTTTTTGCTGTTGTTACATTTTCTGTATTGGTTCAAGGATTGTTCAGCCGCAAGCTTCAACAAGGATCACACTTGATCTGGTCTGGAGACGAAGCATTGGAAATCTTGGAATATTTTGCTATAGCTGTTACAATTGTTGTTGTTGCTGTTCCAGAGGGTTTGCCTTTGGCTGTGACATTGAGTCTTGCTTTTGCCATGAAGAAGATGATGAATGATAAGGCACTAGTCAGGCATCTGGCTGCTTGTGAGACAATGGGATCAGCCACAACTATCTGCAGTGACAAGACTGGGACACTAACGACGAACCACATGACTGTTGTGAAAGCTTGCATTTGTGGTAAAATCAGAGATGTGGGAACCTCCGAGGAAGCTACTAACTTGGCTTCTATAGTTCCTGAATCTGGTTTGAGGCTTATACTGCAATCTATTTTTAACAACACTGGAGGAGAAGTTGTTAAAAACAAAGATGGCAAGACTGAGTTACTTGGAACACCCACTGAGACTGCTATTTTGGAATTTGGGATGTTGCTTGGCGGTGATTGCAAGGCAGAACGGGAAGCAACAAAAGTTGTTAAAGTTGAGCCCTTCAATTCTCATAAAAAGCGAATGGGTGTTGTTCTAGAGCTTCCTGAAGGTGGCTTCCGGGTGCACACTAAAGGTGCTTCTGAGATAATATTAGCTGCATGCGACAAATTCATGGGTCCAGATGGCGAGGTTGTTCCCCTTGATAAAGCATCCATTGATCTTTTGAATGGCATCATTGAACGTTTTGCTAGTGAAGCCCTTAGAACTCTCTGCCTTGCATACATGGAGGTCGGCAATGAGTTCTCAGCTGAAAGTCCTATTCCCACCTCAGGGTACACATGTATAGGCATTGTGGGTATCAAAGATCCAGTTCGTCCTGGTGTCAAGGAGTCTGTAGCAATTTGTAGGTCTGCTGGTATTACAGTCAGAATGGTTACTGGAGACAACATAAACACTGCAAAAGCAATTGCAAGAGAATGTGGAATTTTGACCGATGGAGGTTTGGCAATTGAAGGCCCAGTGTTTCGTGAGAAGAGTGAGGAGGACTTACAAAAAATTATTCCAAACCTTCAGGTTATGGCTCGTTCTTCACCAATGGATAAGCATACCCTTGTAAGACACTTACGGACCACTTTTCAAGAAGTTGTTGCTGTGACTGGTGATGGTACAAACGATGCTCCTGCACTTCATGAAGCTGATATAGGACTTGCAATGGGCATTGCTGGTACTGAGGTGGCAAAAGAAAGTGCTGACGTGATAATTCTGGATGATAACTTTTCTACAATTGTGACTGTGGCCAAATGGGGACGTTCAGTGTACTTGAACATCCAGAAATTTGTTCAGTTCCAGTTAACGGTTAATGTAGTTGCACTTATTGTCAACTTCTCTTCTGCCTGTTTAACTGGAAATACTCCCCTTACTGCTGTTCAGCTTCTCTGGGTCAACATGATCATGGACACTCTAGGAGCCCTTGCATTAGCCACGGAGCCACCTACTGACGATTTAATGAAGAGAGCACCGGTTGGCAGGAAAGGAAACTTCATCACTAATGTGATGTGGAGAAATATCCTAGGGCAGTCTCTGTATCAGTTTGTGATAATATGGTTTCTACAGACCAGAGGAAAAGAAGCTTTTCAGCTTGTTGGCCCAGACTCGGATTTGATCTTGAACACCCTTATCTTCAACTCATTTGTGTTTTGTCAGGTTTTCAATGAGATCAGCTCGAGGGAAATGGAGAAGGTTAACGTCTTCAAAGGCATAATGCAGAACTATGTTTTCGTGACTGTCCTCTCTGCCACTGTTATCTTTCAGATCATAATCATCGAGTTCTTGGGTACATTTGCGAACACACATCCTCTAAGTTGGAAACAATGGTTTGCCAGCGTTGCACTTGGGTTCCTTGGCATGCCGGTTTCAGCAGTTCTGAAATTCATCCCTGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005216 GO:0005261 GO:0005262 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0009507 GO:0009526 GO:0009528 GO:0009536 GO:0009706 GO:0009941 GO:0012505 GO:0015075 GO:0015085 GO:0015267 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0019866 GO:0022803 GO:0022804 GO:0022838 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031224 GO:0031226 GO:0031967 GO:0031969 GO:0031975 GO:0034220 GO:0042170 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase Hydrolase_3

Protein Analysis

1017

Amino Acids

110.87

Weight (kDa)

6.39

Isoelectric Point (pI)

35.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CaATP_NAI	PF12515	5 - 49	2e-21	Ca2+-ATPase N terminal autoinhibitory domain
Cation_ATPase_N	PF00690	116 - 185	3e-11	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	236 - 334	7.9e-21	P-type ATPase actuator domain
Hydrolase	PF00702	451 - 769	7.7e-12	haloacid dehalogenase-like hydrolase
Cation_ATPase	PF13246	503 - 662	7.5e-33	P-type ATPase, cytoplasmic domain N
Cation_ATPase_C	PF00689	840 - 1014	1.2e-41	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0003293)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G27770 AT1G27770 AT1G27770 AT1G27770
fragaria_vesca	FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180 FvH4_2g33180
malus_domestica	MD08G1085200.v1.1 MD08G1085600.v1.1 MD12G1068300.v1.1 MD15G1070800.v1.1
prunus_persica	Prupe.1G423500_v2.0.a1 Prupe.1G423500_v2.0.a1 Prupe.1G423500_v2.0.a1 Prupe.1G423500_v2.0.a1 Prupe.1G423500_v2.0.a1
pyrus_communis	pycom08g07020 pycom15g06650 pycom15g06660
rosa_chinensis	RchiOBHm_Chr6g0309491
rosa_laevigata	RLG00000010561
rosa_multiflora	Rmu_sc0007283.1_g000007
rosa_roxburghii	Rroxscaffold_7G00159360
rosa_rugosa	Rorug06G0372300 Rorug06G0372400
rosa_samantha	Rh6AG485700 Rh6BG495000
rosa_wichuraiana	Rw6G042300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	663
Acc36I	ACCTGC	1 cut(s)	1027
AccB1I	GGYRCC	1 cut(s)	593
AccB7I	CCANNNNNTGG	1 cut(s)	815
AciI	CCGC	4 cut(s)	278, 473, 1132, 1617
AclI	AACGTT	1 cut(s)	1849
AclWI	GGATC	6 cut(s)	159, 316, 946, 1156, 1348, 1967
AcoI	YGGCCR	3 cut(s)	322, 584, 2403
AcsI	RAATTY	8 cut(s)	288, 402, 458, 1598, 1775, 2091, 2441, 3035
AcuI	CTGAAG	5 cut(s)	47, 200, 494, 1734, 2162
AfaI	GTAC	5 cut(s)	1943, 2275, 2335, 2426, 2944
AflIII	ACRYGT	1 cut(s)	1946
AgeI	ACCGGT	1 cut(s)	2626
AhdI	GACNNNNNGTC	1 cut(s)	1263
AhlI	ACTAGT	2 cut(s)	653, 1306
AjiI	CACGTC	1 cut(s)	2362
AjnI	CCWGG	2 cut(s)	769, 1984
AleI	CACNNNNGTG	1 cut(s)	2657
AloI	GAACNNNNNNTCC	2 cut(s)	912, 944
Alw21I	GWGCWC	2 cut(s)	1734, 2626
Alw26I	GTCTC	6 cut(s)	356, 1164, 1325, 1577, 2043, 2691
Alw44I	GTGCAC	1 cut(s)	1730
AlwI	GGATC	6 cut(s)	159, 316, 946, 1156, 1348, 1967
AlwNI	CAGNNNCTG	1 cut(s)	2689
Ama87I	CYCGRG	1 cut(s)	2830
AoxI	GGCC	7 cut(s)	153, 322, 584, 1078, 2122, 2403, 2752
ApaLI	GTGCAC	1 cut(s)	1730
ApeKI	GCWGC	4 cut(s)	209, 275, 1322, 1763
ApoI	RAATTY	8 cut(s)	288, 402, 458, 1598, 1775, 2091, 2441, 3035
ArsI	GACNNNNNNTTYG	4 cut(s)	32, 64, 1576, 1608
AsiGI	ACCGGT	1 cut(s)	2626
Asp700I	GAANNNNTTC	2 cut(s)	507, 2739
AspA2I	CCTAGG	1 cut(s)	2677
AspLEI	GCGC	2 cut(s)	61, 214
AspS9I	GGNCC	5 cut(s)	805, 1784, 2123, 2235, 2753
AsuC2I	CCSGG	1 cut(s)	1727
AsuHPI	GGTGA	3 cut(s)	1631, 2190, 2279
AsuNHI	GCTAGC	1 cut(s)	620
AvaI	CYCGRG	1 cut(s)	2830
AvaII	GGWCC	3 cut(s)	805, 1784, 2235
AvrII	CCTAGG	1 cut(s)	2677
AxyI	CCTNAGG	2 cut(s)	261, 1936
BaeGI	GKGCMC	2 cut(s)	598, 1734
BaeI	ACNNNNGTAYC	4 cut(s)	1933, 1966, 2325, 2358
BalI	TGGCCA	2 cut(s)	586, 2405
BanI	GGYRCC	1 cut(s)	593
BanII	GRGCYC	2 cut(s)	1668, 2578
BbsI	GAAGAC	1 cut(s)	2848
Bbv12I	GWGCWC	2 cut(s)	1734, 2626
BbvCI	CCTCAGC	1 cut(s)	150
BbvI	GCAGC	4 cut(s)	196, 262, 1309, 1750
BceAI	ACGGC	1 cut(s)	309
BcgI	CGANNNNNNTGC	2 cut(s)	2270, 2304
BciT130I	CCWGG	2 cut(s)	771, 1986
BclI	TGATCA	1 cut(s)	2556
BcnI	CCSGG	1 cut(s)	1727
BcoDI	GTCTC	6 cut(s)	356, 1164, 1325, 1577, 2043, 2691
BcuI	ACTAGT	2 cut(s)	653, 1306
BfaI	CTAG	8 cut(s)	621, 654, 726, 1307, 1703, 1857, 2570, 2678
BfmI	CTRYAG	6 cut(s)	674, 1203, 1357, 1470, 2001, 2717
BfoI	RGCGCY	1 cut(s)	62
BfuAI	ACCTGC	1 cut(s)	1027
BglII	AGATCT	1 cut(s)	799
BisI	GCNGC	5 cut(s)	210, 276, 1132, 1323, 1764
BlnI	CCTAGG	1 cut(s)	2677
BlsI	GCNGC	5 cut(s)	211, 277, 1133, 1324, 1765
Bme1390I	CCNGG	3 cut(s)	771, 1727, 1986
Bme18I	GGWCC	3 cut(s)	805, 1784, 2235
BmeRI	GACNNNNNGTC	1 cut(s)	1263
BmeT110I	CYCGRG	1 cut(s)	2830
BmgBI	CACGTC	1 cut(s)	2362
BmgT120I	GGNCC	5 cut(s)	805, 1784, 2123, 2235, 2753
BmiI	GGNNCC	7 cut(s)	595, 807, 1441, 1785, 2575, 2595, 3005
BmrFI	CCNGG	3 cut(s)	771, 1727, 1986
BmrI	ACTGGG	4 cut(s)	237, 973, 1380, 2120
BmsI	GCATC	5 cut(s)	939, 1324, 1826, 1848, 2272
BmtI	GCTAGC	1 cut(s)	624
BmuI	ACTGGG	4 cut(s)	237, 973, 1380, 2120
BpiI	GAAGAC	1 cut(s)	2848
BplI	GAGNNNNNCTC	2 cut(s)	2813, 2845
BpmI	CTGGAG	3 cut(s)	1188, 1545, 2067
Bpu10I	CCTNAGC	1 cut(s)	150
BpuMI	CCSGG	1 cut(s)	1727
BsaBI	GATNNNNATC	1 cut(s)	2924
BsaJI	CCNNGG	6 cut(s)	226, 478, 1446, 2589, 2677, 3007
BsaWI	WCCGGW	1 cut(s)	2626
BsaXI	ACNNNNNCTCC	6 cut(s)	789, 819, 1518, 1521, 1548, 1551
Bse118I	RCCGGY	2 cut(s)	2626, 3016
Bse21I	CCTNAGG	2 cut(s)	261, 1936
Bse3DI	GCAATG	3 cut(s)	1909, 2325, 2325
Bse8I	GATNNNNATC	1 cut(s)	2924
BseBI	CCWGG	2 cut(s)	771, 1986
BseDI	CCNNGG	6 cut(s)	226, 478, 1446, 2589, 2677, 3007
BseGI	GGATG	8 cut(s)	386, 940, 1611, 1817, 2380, 2433, 2959, 3039
BseJI	GATNNNNATC	1 cut(s)	2924
BseMI	GCAATG	3 cut(s)	1909, 2325, 2325
BseMII	CTCAG	9 cut(s)	141, 252, 405, 1551, 1572, 1740, 1926, 1950, 2328
BseRI	GAGGAG	5 cut(s)	68, 902, 1029, 1542, 2162
BseSI	GKGCMC	2 cut(s)	598, 1734
BseXI	GCAGC	4 cut(s)	196, 262, 1309, 1750
BseYI	CCCAGC	1 cut(s)	808
BsgI	GTGCAG	1 cut(s)	2274
BshFI	GGCC	7 cut(s)	155, 324, 586, 1080, 2124, 2405, 2754
BshNI	GGYRCC	1 cut(s)	593
BshTI	ACCGGT	1 cut(s)	2626
BsiHKAI	GWGCWC	2 cut(s)	1734, 2626
BsiHKCI	CYCGRG	1 cut(s)	2830
BsiSI	CCGG	3 cut(s)	1726, 2627, 3017
BslFI	GGGAC	3 cut(s)	791, 1387, 2427
BsmAI	GTCTC	6 cut(s)	356, 1164, 1325, 1577, 2043, 2691
BsmBI	CGTCTC	1 cut(s)	1164
BsmFI	GGGAC	3 cut(s)	791, 1387, 2427
BsnI	GGCC	7 cut(s)	155, 324, 586, 1080, 2124, 2405, 2754
BsoBI	CYCGRG	1 cut(s)	2830
Bsp1286I	GDGCHC	5 cut(s)	598, 1668, 1734, 2578, 2626
BspACI	CCGC	4 cut(s)	278, 473, 1132, 1617
BspANI	GGCC	7 cut(s)	155, 324, 586, 1080, 2124, 2405, 2754
BspCNI	CTCAG	9 cut(s)	142, 253, 406, 1552, 1573, 1741, 1925, 1949, 2329
BspHI	TCATGA	2 cut(s)	525, 2296
BspLI	GGNNCC	7 cut(s)	595, 807, 1441, 1785, 2575, 2595, 3005
BspMAI	CTGCAG	2 cut(s)	678, 1361
BspMI	ACCTGC	1 cut(s)	1027
BspOI	GCTAGC	1 cut(s)	624
BspPI	GGATC	6 cut(s)	159, 316, 946, 1156, 1348, 1967
BspT107I	GGYRCC	1 cut(s)	593
BsrDI	GCAATG	3 cut(s)	1909, 2325, 2325
BsrFI	RCCGGY	2 cut(s)	2626, 3016
BssAI	RCCGGY	2 cut(s)	2626, 3016
BssECI	CCNNGG	6 cut(s)	226, 478, 1446, 2589, 2677, 3007
BssT1I	CCWWGG	3 cut(s)	226, 2677, 3007
Bst2UI	CCWGG	2 cut(s)	771, 1986
Bst4CI	ACNGT	9 cut(s)	256, 715, 964, 1399, 2032, 2401, 2464, 2893, 2908
Bst6I	CTCTTC	3 cut(s)	2135, 2500, 2612
BstAPI	GCANNNNNTGC	1 cut(s)	542
BstC8I	GCNNGC	6 cut(s)	622, 1136, 1411, 1768, 2989, 3014
BstDSI	CCRYGG	2 cut(s)	478, 2589
BstF5I	GGATG	8 cut(s)	386, 940, 1611, 1817, 2380, 2433, 2959, 3039
BstH2I	RGCGCY	1 cut(s)	62
BstHHI	GCGC	2 cut(s)	61, 214
BstMAI	GTCTC	6 cut(s)	356, 1164, 1325, 1577, 2043, 2691
BstMWI	GCNNNNNNNGC	5 cut(s)	542, 1501, 1623, 1862, 2993
BstNI	CCWGG	2 cut(s)	771, 1986
BstNSI	RCATGY	3 cut(s)	1770, 1950, 3016
BstSCI	CCNGG	3 cut(s)	769, 1725, 1984
BstSFI	CTRYAG	6 cut(s)	674, 1203, 1357, 1470, 2001, 2717
BstSLI	GKGCMC	2 cut(s)	598, 1734
BstV1I	GCAGC	4 cut(s)	196, 262, 1309, 1750
BstV2I	GAAGAC	1 cut(s)	2848
BstX2I	RGATCY	2 cut(s)	799, 1972
BstYI	RGATCY	2 cut(s)	799, 1972
Bsu36I	CCTNAGG	2 cut(s)	261, 1936
BsuRI	GGCC	7 cut(s)	155, 324, 586, 1080, 2124, 2405, 2754
BtgI	CCRYGG	2 cut(s)	478, 2589
BtrI	CACGTC	1 cut(s)	2362
BtsCI	GGATG	8 cut(s)	386, 940, 1611, 1817, 2380, 2433, 2959, 3039
BtsI	GCAGTG	4 cut(s)	120, 645, 1366, 2061
BveI	ACCTGC	1 cut(s)	1027
Cac8I	GCNNGC	6 cut(s)	622, 1136, 1411, 1768, 2989, 3014
CaiI	CAGNNNCTG	1 cut(s)	2689
CciI	TCATGA	2 cut(s)	525, 2296
CfoI	GCGC	2 cut(s)	61, 214
Cfr10I	RCCGGY	2 cut(s)	2626, 3016
Cfr13I	GGNCC	5 cut(s)	805, 1784, 2123, 2235, 2753
Csp6I	GTAC	5 cut(s)	1942, 2274, 2334, 2425, 2943
CspAI	ACCGGT	1 cut(s)	2626
CviQI	GTAC	5 cut(s)	1942, 2274, 2334, 2425, 2943
DriI	GACNNNNNGTC	1 cut(s)	1263
EaeI	YGGCCR	3 cut(s)	322, 584, 2403
Eam1104I	CTCTTC	3 cut(s)	2135, 2500, 2612
Eam1105I	GACNNNNNGTC	1 cut(s)	1263
EarI	CTCTTC	3 cut(s)	2135, 2500, 2612
Eco130I	CCWWGG	3 cut(s)	226, 2677, 3007
Eco147I	AGGCCT	1 cut(s)	1080
Eco24I	GRGCYC	2 cut(s)	1668, 2578
Eco47I	GGWCC	3 cut(s)	805, 1784, 2235
Eco57I	CTGAAG	5 cut(s)	47, 200, 494, 1734, 2162
Eco81I	CCTNAGG	2 cut(s)	261, 1936
Eco88I	CYCGRG	1 cut(s)	2830
EcoRII	CCWGG	2 cut(s)	769, 1984
EcoT14I	CCWWGG	3 cut(s)	226, 2677, 3007
EcoT38I	GRGCYC	2 cut(s)	1668, 2578
ErhI	CCWWGG	3 cut(s)	226, 2677, 3007
Esp3I	CGTCTC	1 cut(s)	1164
FalI	AAGNNNNNCTT	2 cut(s)	198, 230
FaqI	GGGAC	3 cut(s)	791, 1387, 2427
FauI	CCCGC	1 cut(s)	271
FbaI	TGATCA	1 cut(s)	2556
Fnu4HI	GCNGC	5 cut(s)	210, 276, 1132, 1323, 1764
FokI	GGATG	8 cut(s)	373, 947, 1618, 1804, 2387, 2420, 2946, 3026
FriOI	GRGCYC	2 cut(s)	1668, 2578
Fsp4HI	GCNGC	5 cut(s)	210, 276, 1132, 1323, 1764
FspBI	CTAG	8 cut(s)	621, 654, 726, 1307, 1703, 1857, 2570, 2678
GlaI	GCGC	2 cut(s)	60, 213
GluI	GCNGC	5 cut(s)	210, 276, 1132, 1323, 1764
GsaI	CCCAGC	1 cut(s)	812
GsuI	CTGGAG	3 cut(s)	1188, 1545, 2067
HaeII	RGCGCY	1 cut(s)	62
HaeIII	GGCC	7 cut(s)	155, 324, 586, 1080, 2124, 2405, 2754
HapII	CCGG	3 cut(s)	1726, 2627, 3017
HhaI	GCGC	2 cut(s)	61, 214
Hin6I	GCGC	2 cut(s)	59, 212
HinP1I	GCGC	2 cut(s)	59, 212
HincII	GTYRAC	5 cut(s)	892, 2460, 2488, 2551, 2851
HindII	GTYRAC	5 cut(s)	892, 2460, 2488, 2551, 2851
HindIII	AAGCTT	4 cut(s)	377, 1136, 1407, 2736
HinfI	GANTC	9 cut(s)	97, 117, 735, 854, 901, 1264, 1481, 1997, 2759
HpaI	GTTAAC	2 cut(s)	2460, 2851
HpaII	CCGG	3 cut(s)	1726, 2627, 3017
HphI	GGTGA	3 cut(s)	1631, 2190, 2279
Hpy99I	CGWCG	3 cut(s)	52, 165, 1387
HpyCH4III	ACNGT	9 cut(s)	256, 715, 964, 1399, 2032, 2401, 2464, 2893, 2908
HpyCH4IV	ACGT	4 cut(s)	1849, 2361, 2416, 2853
HpyF10VI	GCNNNNNNNGC	5 cut(s)	542, 1501, 1623, 1862, 2993
HpySE526I	ACGT	4 cut(s)	1849, 2361, 2416, 2853
HspAI	GCGC	2 cut(s)	59, 212
Ksp22I	TGATCA	1 cut(s)	2556
KspAI	GTTAAC	2 cut(s)	2460, 2851
LmnI	GCTCC	3 cut(s)	2290, 2573, 2593
Lsp1109I	GCAGC	4 cut(s)	196, 262, 1309, 1750
LweI	GCATC	5 cut(s)	939, 1324, 1826, 1848, 2272
MaeI	CTAG	8 cut(s)	621, 654, 726, 1307, 1703, 1857, 2570, 2678
MaeII	ACGT	4 cut(s)	1849, 2361, 2416, 2853
MfeI	CAATTG	5 cut(s)	312, 1215, 2073, 2115, 2391
MflI	RGATCY	2 cut(s)	799, 1972
MhlI	GDGCHC	5 cut(s)	598, 1668, 1734, 2578, 2626
MlsI	TGGCCA	2 cut(s)	586, 2405
MluNI	TGGCCA	2 cut(s)	586, 2405
MlyI	GAGTC	3 cut(s)	1273, 2006, 2753
MmeI	TCCRAC	3 cut(s)	30, 946, 2951
Mox20I	TGGCCA	2 cut(s)	586, 2405
MroXI	GAANNNNTTC	2 cut(s)	507, 2739
MscI	TGGCCA	2 cut(s)	586, 2405
MslI	CAYNNNNRTG	4 cut(s)	354, 2558, 2657, 3044
Msp20I	TGGCCA	2 cut(s)	586, 2405
MspA1I	CMGCKG	2 cut(s)	812, 1916
MspI	CCGG	3 cut(s)	1726, 2627, 3017
MspR9I	CCNGG	3 cut(s)	771, 1727, 1986
MunI	CAATTG	5 cut(s)	312, 1215, 2073, 2115, 2391
MvaI	CCWGG	2 cut(s)	771, 1986
MwoI	GCNNNNNNNGC	5 cut(s)	542, 1501, 1623, 1862, 2993
NciI	CCSGG	1 cut(s)	1727
NheI	GCTAGC	1 cut(s)	620
NlaIV	GGNNCC	7 cut(s)	595, 807, 1441, 1785, 2575, 2595, 3005
NmuCI	GTSAC	6 cut(s)	643, 1255, 1361, 2260, 2395, 2887
NspI	RCATGY	3 cut(s)	1770, 1950, 3016
OliI	CACNNNNGTG	1 cut(s)	2657
PaeI	GCATGC	2 cut(s)	1770, 3016
PaeR7I	CTCGAG	1 cut(s)	2830
PagI	TCATGA	2 cut(s)	525, 2296
PceI	AGGCCT	1 cut(s)	1080
PciI	ACATGT	1 cut(s)	1946
PdmI	GAANNNNTTC	2 cut(s)	507, 2739
PfeI	GAWTC	6 cut(s)	97, 117, 735, 854, 901, 1481
PflMI	CCANNNNNTGG	1 cut(s)	815
PinAI	ACCGGT	1 cut(s)	2626
PkrI	GCNGC	5 cut(s)	211, 277, 1133, 1324, 1765
PleI	GAGTC	3 cut(s)	1272, 2005, 2753
PpsI	GAGTC	3 cut(s)	1272, 2005, 2753
PscI	ACATGT	1 cut(s)	1946
PsiI	TTATAA	1 cut(s)	663
Psp1406I	AACGTT	1 cut(s)	1849
Psp6I	CCWGG	2 cut(s)	769, 1984
PspFI	CCCAGC	1 cut(s)	808
PspGI	CCWGG	2 cut(s)	769, 1984
PspN4I	GGNNCC	7 cut(s)	595, 807, 1441, 1785, 2575, 2595, 3005
PspPI	GGNCC	5 cut(s)	805, 1784, 2123, 2235, 2753
PspXI	VCTCGAGB	1 cut(s)	2830
PstI	CTGCAG	2 cut(s)	678, 1361
PstNI	CAGNNNCTG	1 cut(s)	2689
PsuI	RGATCY	2 cut(s)	799, 1972
PvuII	CAGCTG	2 cut(s)	812, 1916
RsaI	GTAC	5 cut(s)	1943, 2275, 2335, 2426, 2944
RsaNI	GTAC	5 cut(s)	1942, 2274, 2334, 2425, 2943
RseI	CAYNNNNRTG	4 cut(s)	354, 2558, 2657, 3044
SatI	GCNGC	5 cut(s)	210, 276, 1132, 1323, 1764
Sau96I	GGNCC	5 cut(s)	805, 1784, 2123, 2235, 2753
SchI	GAGTC	3 cut(s)	1273, 2006, 2753
ScrFI	CCNGG	3 cut(s)	771, 1727, 1986
SduI	GDGCHC	5 cut(s)	598, 1668, 1734, 2578, 2626
SfaNI	GCATC	5 cut(s)	939, 1324, 1826, 1848, 2272
SfcI	CTRYAG	6 cut(s)	674, 1203, 1357, 1470, 2001, 2717
Sfr274I	CTCGAG	1 cut(s)	2830
SinI	GGWCC	3 cut(s)	805, 1784, 2235
SlaI	CTCGAG	1 cut(s)	2830
SmiMI	CAYNNNNRTG	4 cut(s)	354, 2558, 2657, 3044
SmlI	CTYRAG	1 cut(s)	2830
SmoI	CTYRAG	1 cut(s)	2830
SpeI	ACTAGT	2 cut(s)	653, 1306
SphI	GCATGC	2 cut(s)	1770, 3016
SseBI	AGGCCT	1 cut(s)	1080
SsiI	CCGC	4 cut(s)	278, 473, 1132, 1617
SspI	AATATT	2 cut(s)	1196, 1758
SspMI	CTAG	8 cut(s)	621, 654, 726, 1307, 1703, 1857, 2570, 2678
StuI	AGGCCT	1 cut(s)	1080
StyD4I	CCNGG	3 cut(s)	769, 1725, 1984
StyI	CCWWGG	3 cut(s)	226, 2677, 3007
TaaI	ACNGT	9 cut(s)	256, 715, 964, 1399, 2032, 2401, 2464, 2893, 2908
TaiI	ACGT	4 cut(s)	1852, 2364, 2419, 2856
TaqI	TCGA	3 cut(s)	160, 2831, 2931
TaqII	GACCGA	1 cut(s)	2114
TatI	WGTACW	1 cut(s)	2424
TauI	GCSGC	1 cut(s)	1134
TfiI	GAWTC	6 cut(s)	97, 117, 735, 854, 901, 1481
TseFI	GTSAC	6 cut(s)	643, 1255, 1361, 2260, 2395, 2887
TseI	GCWGC	4 cut(s)	209, 275, 1322, 1763
Tsp45I	GTSAC	6 cut(s)	643, 1255, 1361, 2260, 2395, 2887
TspGWI	ACGGA	3 cut(s)	121, 2248, 2606
Van91I	CCANNNNNTGG	1 cut(s)	815
VneI	GTGCAC	1 cut(s)	1730
VpaK11BI	GGWCC	3 cut(s)	805, 1784, 2235
XapI	RAATTY	8 cut(s)	288, 402, 458, 1598, 1775, 2091, 2441, 3035
XbaI	TCTAGA	1 cut(s)	1702
XceI	RCATGY	3 cut(s)	1770, 1950, 3016
XcmI	CCANNNNNNNNNTGG	1 cut(s)	605
XhoI	CTCGAG	1 cut(s)	2830
XmaJI	CCTAGG	1 cut(s)	2677
XmnI	GAANNNNTTC	2 cut(s)	507, 2739
XspI	CTAG	8 cut(s)	621, 654, 726, 1307, 1703, 1857, 2570, 2678

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.