Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0007140

Physical Location & Seq

Genomic Coordinates Forward (+)

25948502 .. 25951954

Gene Structure 3453 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g35110.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1638 bp

ATGGGTTTGGGTTTTAGGCTGAACCTCAAGTGTGGTTTTCCTATTCATAATTCTCATTGGCAAACCAATTCTCCTTCCAAGCTTCCATCTTCTTCTGTTAGAGGCAGGGAAGGCTCTTTCAGTTCAGCAGGGCTGTCCTTTCTGTCTGTTATATCTGAGGAATGTGAGACTTCTAGTAAAGATCCTCTGAAAGTGTACACGTGTCCATCTGCATCCCCACAACTCTTGGATGATGGTTTATCAGAAATGGAAGAGAAGATGAACCCTAGGAGGATGTCAAAGAGTGGCTCAGCTAACATGATTGATGATAAAGAGTACAGCTTTATGTCCAGTGCAGGGTTAAGAACATCTCATTATACCTTGTTACTGCAGAATCTTAATGTTTTGGAGGAGACTTTTGCTGATTCAGGTGTGTCAAAGTTGGAAAAGGAGATCCTGTTGCAATTAGGAAGGCTCGGAGCTCTGAAGTTATTTGAGATCTGTCTATCAAGAACGCTTAAAACCTCGAGCTTTTTGGATTTATCTGACATACCTACTGAACCGATTGAAAAGCATAAGACGGAAAGGAAGAATATTGTCCGTTCCGGCAAAAAGGAAGAGCAAAAATCAAGGAAAAGAACATCATATGATTCCAAAAAGTTTTCTGGTACCTTGCCTGTGAAACAAATGAGGGAAGGTTTTAAAAAGCCTAATCCTTCATCAGTAAGAAGAGCATCAAACTCTAGAAGCCGAAGACTTATGATGGCTAAAAATGAGGCAGAAATGTCAGTTGGAGTTAAGGTGATCGCCGACTTGGAGAGGCTTAAAGTAACATTAGAGAAGGAAACCGGTAAGGTACCGACCTTAAGATGCTGGGCAGAAGCAGCTGGAGTTGATGAAAAGGTGCTGCAACAACAACTGCATTTCGGCTGGTACTGCCAAGATGAGCTTGTAAGGAGCACTCGGTCCTTAGTTCTGTACCTTGCTCGAAATTATAGAGGACTTGGAGTAGCCTTAGATGATTTGTTACAGGCTGGAAACCTGGGTCTCCTACAGGGTGCTGAAAGGTTTGATCCCTCACGGGGTTACAGATTCTCAACCTATGTCCAATACTGGATAAGAAAATCAATGTCAAGATTGGTGGCACAACATGCCAGAGGGATCAAAATTCCTAACACGCTTAGTAAGGCAATAAATCGGATACAGAAGGCTCGAAAAGCCAATTACAACAGCCACATGAAATACCCAGATGATGAAGAAATAGCAAAGATTACAGGTCTTTCTTTGGCGAAAATCAGCTCGGCTAGCAGATGTCTTAGAGTTGTGGGTTCGGTAGATCATAAGATATGGGAATGCTCGGCCGGGACATATATGGAATTTACTCCGGATATAGCAGTAGAGACTCCTGAAGAATCTGTCATGCGGCAGCACATGAAAAACGATATTCATGTTCTGCTGGAAGGCTTGAATTCAAGAGAAAAGAAAGTTTTGGCACTACGATATGGTCTAAACTACTGCAACCCCAAGTCACTTGAGGAGATCGGAAGGCTTTTCCATGTGAGCAAGGAGTGGATACGAAAGATAGAAAAGAATGCTCTGACCAAGCTGAGGAATGAAGAAAGCAACAAAAACTTGAGCCATTATTTGAAGCAGTGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000988 GO:0000990 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006351 GO:0006352 GO:0006355 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009416 GO:0009507 GO:0009536 GO:0009628 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0016987 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031326 GO:0032774 GO:0034062 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043254 GO:0044087 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051252 GO:0051716 GO:0060255 GO:0065007 GO:0071214 GO:0071478 GO:0071482 GO:0071704 GO:0080090 GO:0090304 GO:0097659 GO:0097747 GO:0104004 GO:0140098 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1903506 GO:2000112 GO:2000142 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Sigma70_r2 Sigma70_r3 Sigma70_r4

Protein Analysis

546

Amino Acids

61.84

Weight (kDa)

9.55

Isoelectric Point (pI)

49.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Sigma70_r2	PF04542	310 - 379	4.6e-16	Sigma-70 region 2
Sigma70_r3	PF04539	390 - 465	4.9e-08	Sigma-70 region 3
Sigma70_r4	PF04545	478 - 531	8.1e-14	Sigma-70, region 4

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007140)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G53920 AT3G53920
fragaria_vesca	FvH4_2g35110 FvH4_2g35110 FvH4_2g35110
malus_domestica	MD08G1108600.v1.1 MD15G1089100.v1.1
prunus_persica	Prupe.1G443200_v2.0.a1 Prupe.1G443200_v2.0.a1 Prupe.1G443200_v2.0.a1
pyrus_communis	pycom08g09050 pycom15g08350
rosa_chinensis	RchiOBHm_Chr6g0306861
rosa_laevigata	RLG00000010760
rosa_multiflora	Rmu_sc0009381.1_g000005
rosa_roxburghii	Rroxscaffold_7G00161410
rosa_rugosa	Rorug06G0353600 Rorug06G0353700 Rorug06G0353800 Rorug06G0353900
rosa_samantha	Rh6AG465400 Rh6BG435400 Rh6CG479800 Rh6DG466800
rosa_wichuraiana	Rw6G040560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	2 cut(s)	647, 835
AccB1I	GGYRCC	2 cut(s)	647, 835
AccIII	TCCGGA	1 cut(s)	1363
AciI	CCGC	1 cut(s)	1402
AclWI	GGATC	4 cut(s)	176, 427, 1046, 1148
AcoI	YGGCCR	1 cut(s)	1338
AcsI	RAATTY	3 cut(s)	1146, 1355, 1447
AcuI	CTGAAG	2 cut(s)	485, 1407
AcvI	CACGTG	1 cut(s)	201
AfaI	GTAC	6 cut(s)	197, 317, 649, 837, 914, 959
AfiI	CCNNNNNNNGG	3 cut(s)	336, 1061, 1587
AflII	CTTAAG	1 cut(s)	844
AflIII	ACRYGT	2 cut(s)	198, 200
AgeI	ACCGGT	1 cut(s)	827
AgsI	TTSAA	4 cut(s)	548, 1447, 1452, 1627
AjnI	CCWGG	1 cut(s)	1020
AjuI	GAANNNNNNNTTGG	2 cut(s)	753, 785
AluBI	AGCT	9 cut(s)	82, 293, 321, 461, 510, 866, 928, 1278, 1585
AluI	AGCT	9 cut(s)	82, 293, 321, 461, 510, 866, 928, 1278, 1585
Alw21I	GWGCWC	2 cut(s)	463, 941
Alw26I	GTCTC	4 cut(s)	161, 386, 1031, 1373
AlwI	GGATC	4 cut(s)	176, 427, 1046, 1148
Ama87I	CYCGRG	1 cut(s)	505
Aor13HI	TCCGGA	1 cut(s)	1363
AoxI	GGCC	1 cut(s)	1338
ApeKI	GCWGC	3 cut(s)	863, 886, 1405
ApoI	RAATTY	3 cut(s)	1146, 1355, 1447
AsiGI	ACCGGT	1 cut(s)	827
Asp718I	GGTACC	2 cut(s)	647, 835
AspA2I	CCTAGG	1 cut(s)	266
AspS9I	GGNCC	1 cut(s)	945
AsuC2I	CCSGG	1 cut(s)	1342
AsuHPI	GGTGA	1 cut(s)	793
AsuNHI	GCTAGC	1 cut(s)	1283
AvaI	CYCGRG	1 cut(s)	505
AvaII	GGWCC	1 cut(s)	945
AvrII	CCTAGG	1 cut(s)	266
BanI	GGYRCC	2 cut(s)	647, 835
BanII	GRGCYC	1 cut(s)	463
BbrPI	CACGTG	1 cut(s)	201
BbsI	GAAGAC	1 cut(s)	739
Bbv12I	GWGCWC	2 cut(s)	463, 941
BbvCI	CCTCAGC	1 cut(s)	1586
BbvI	GCAGC	3 cut(s)	873, 875, 1417
BccI	CCATC	4 cut(s)	94, 214, 227, 736
BciT130I	CCWGG	1 cut(s)	1022
BciVI	GTATCC	2 cut(s)	1173, 1545
BcnI	CCSGG	1 cut(s)	1342
BcoDI	GTCTC	4 cut(s)	161, 386, 1031, 1373
BfaI	CTAG	4 cut(s)	174, 267, 723, 1284
BfmI	CTRYAG	2 cut(s)	368, 1031
BfrI	CTTAAG	1 cut(s)	844
BfuI	GTATCC	2 cut(s)	1173, 1545
BglII	AGATCT	1 cut(s)	477
BisI	GCNGC	4 cut(s)	864, 887, 1403, 1406
BlnI	CCTAGG	1 cut(s)	266
BlpI	GCTNAGC	1 cut(s)	289
BlsI	GCNGC	4 cut(s)	865, 888, 1404, 1407
Bme1390I	CCNGG	2 cut(s)	1022, 1342
Bme18I	GGWCC	1 cut(s)	945
BmeT110I	CYCGRG	1 cut(s)	505
BmgT120I	GGNCC	1 cut(s)	945
BmiI	GGNNCC	2 cut(s)	649, 837
BmrFI	CCNGG	2 cut(s)	1022, 1342
BmsI	GCATC	3 cut(s)	221, 722, 839
BmtI	GCTAGC	1 cut(s)	1287
BpiI	GAAGAC	1 cut(s)	739
BpmI	CTGGAG	1 cut(s)	888
Bpu10I	CCTNAGC	1 cut(s)	1586
Bpu1102I	GCTNAGC	1 cut(s)	289
BpuEI	CTTGAG	3 cut(s)	11, 1532, 1633
BpuMI	CCSGG	1 cut(s)	1342
BsaAI	YACGTR	1 cut(s)	201
BsaI	GGTCTC	1 cut(s)	1031
BsaJI	CCNNGG	2 cut(s)	266, 1021
BsaWI	WCCGGW	2 cut(s)	827, 1363
BsaXI	ACNNNNNCTCC	6 cut(s)	55, 85, 422, 452, 765, 795
Bsc4I	CCNNNNNNNGG	3 cut(s)	336, 1061, 1587
Bse118I	RCCGGY	1 cut(s)	827
Bse1I	ACTGG	2 cut(s)	330, 1097
BseAI	TCCGGA	1 cut(s)	1363
BseBI	CCWGG	1 cut(s)	1022
BseDI	CCNNGG	2 cut(s)	266, 1021
BseGI	GGATG	3 cut(s)	212, 235, 279
BseLI	CCNNNNNNNGG	3 cut(s)	336, 1061, 1587
BseMII	CTCAG	3 cut(s)	147, 303, 1577
BseNI	ACTGG	2 cut(s)	330, 1097
BseRI	GAGGAG	2 cut(s)	404, 1529
BseX3I	CGGCCG	1 cut(s)	1338
BseXI	GCAGC	3 cut(s)	873, 875, 1417
BseYI	CCCAGC	1 cut(s)	852
BsgI	GTGCAG	1 cut(s)	354
Bsh1285I	CGRYCG	1 cut(s)	1341
BshFI	GGCC	1 cut(s)	1340
BshNI	GGYRCC	2 cut(s)	647, 835
BshTI	ACCGGT	1 cut(s)	827
BsiEI	CGRYCG	1 cut(s)	1341
BsiHKAI	GWGCWC	2 cut(s)	463, 941
BsiHKCI	CYCGRG	1 cut(s)	505
BsiSI	CCGG	4 cut(s)	585, 828, 1341, 1364
BslFI	GGGAC	1 cut(s)	1357
BslI	CCNNNNNNNGG	3 cut(s)	336, 1061, 1587
BsmAI	GTCTC	4 cut(s)	161, 386, 1031, 1373
BsmFI	GGGAC	1 cut(s)	1357
BsmI	GAATGC	2 cut(s)	1337, 1576
BsnI	GGCC	1 cut(s)	1340
Bso31I	GGTCTC	1 cut(s)	1031
BsoBI	CYCGRG	1 cut(s)	505
Bsp1286I	GDGCHC	2 cut(s)	463, 941
Bsp13I	TCCGGA	1 cut(s)	1363
Bsp1407I	TGTACA	1 cut(s)	195
Bsp143I	GATC	8 cut(s)	181, 432, 477, 783, 1051, 1140, 1315, 1518
Bsp1720I	GCTNAGC	1 cut(s)	289
BspACI	CCGC	1 cut(s)	1402
BspANI	GGCC	1 cut(s)	1340
BspCNI	CTCAG	3 cut(s)	148, 302, 1578
BspEI	TCCGGA	1 cut(s)	1363
BspLI	GGNNCC	2 cut(s)	649, 837
BspMAI	CTGCAG	1 cut(s)	372
BspOI	GCTAGC	1 cut(s)	1287
BspPI	GGATC	4 cut(s)	176, 427, 1046, 1148
BspQI	GCTCTTC	2 cut(s)	591, 703
BspT107I	GGYRCC	2 cut(s)	647, 835
BspTI	CTTAAG	1 cut(s)	844
BspTNI	GGTCTC	1 cut(s)	1031
BsrFI	RCCGGY	1 cut(s)	827
BsrGI	TGTACA	1 cut(s)	195
BsrI	ACTGG	2 cut(s)	330, 1097
BssAI	RCCGGY	1 cut(s)	827
BssECI	CCNNGG	2 cut(s)	266, 1021
BssMI	GATC	8 cut(s)	181, 432, 477, 783, 1051, 1140, 1315, 1518
BssT1I	CCWWGG	1 cut(s)	266
Bst2UI	CCWGG	1 cut(s)	1022
Bst6I	CTCTTC	3 cut(s)	246, 591, 703
BstAFI	CTTAAG	1 cut(s)	844
BstAPI	GCANNNNNTGC	1 cut(s)	1130
BstAUI	TGTACA	1 cut(s)	195
BstBAI	YACGTR	1 cut(s)	201
BstC8I	GCNNGC	1 cut(s)	1285
BstDEI	CTNAG	7 cut(s)	156, 289, 949, 994, 1160, 1295, 1586
BstF5I	GGATG	3 cut(s)	212, 235, 279
BstKTI	GATC	8 cut(s)	184, 435, 480, 786, 1054, 1143, 1318, 1521
BstMAI	GTCTC	4 cut(s)	161, 386, 1031, 1373
BstMBI	GATC	8 cut(s)	181, 432, 477, 783, 1051, 1140, 1315, 1518
BstMCI	CGRYCG	1 cut(s)	1341
BstMWI	GCNNNNNNNGC	6 cut(s)	111, 863, 915, 1130, 1196, 1284
BstNI	CCWGG	1 cut(s)	1022
BstNSI	RCATGY	1 cut(s)	1133
BstSCI	CCNGG	2 cut(s)	1020, 1340
BstSFI	CTRYAG	2 cut(s)	368, 1031
BstV1I	GCAGC	3 cut(s)	873, 875, 1417
BstV2I	GAAGAC	1 cut(s)	739
BstX2I	RGATCY	3 cut(s)	181, 432, 477
BstYI	RGATCY	3 cut(s)	181, 432, 477
BstZI	CGGCCG	1 cut(s)	1338
BsuI	GTATCC	2 cut(s)	1173, 1545
BsuRI	GGCC	1 cut(s)	1340
BtsCI	GGATG	3 cut(s)	212, 235, 279
BtsI	GCAGTG	1 cut(s)	1637
BtsIMutI	CAGTG	2 cut(s)	337, 1637
Cac8I	GCNNGC	1 cut(s)	1285
Cfr10I	RCCGGY	1 cut(s)	827
Cfr13I	GGNCC	1 cut(s)	945
Csp6I	GTAC	6 cut(s)	196, 316, 648, 836, 913, 958
CspAI	ACCGGT	1 cut(s)	827
CspCI	CAANNNNNGTGG	4 cut(s)	207, 242, 1101, 1136
CviAII	CATG	7 cut(s)	298, 1130, 1216, 1399, 1411, 1427, 1535
CviQI	GTAC	6 cut(s)	196, 316, 648, 836, 913, 958
DdeI	CTNAG	7 cut(s)	156, 289, 949, 994, 1160, 1295, 1586
DpnI	GATC	8 cut(s)	183, 434, 479, 785, 1053, 1142, 1317, 1520
DpnII	GATC	8 cut(s)	181, 432, 477, 783, 1051, 1140, 1315, 1518
DraI	TTTAAA	1 cut(s)	682
EaeI	YGGCCR	1 cut(s)	1338
EagI	CGGCCG	1 cut(s)	1338
Eam1104I	CTCTTC	3 cut(s)	246, 591, 703
EarI	CTCTTC	3 cut(s)	246, 591, 703
Ecl136II	GAGCTC	1 cut(s)	461
EclXI	CGGCCG	1 cut(s)	1338
Eco130I	CCWWGG	1 cut(s)	266
Eco24I	GRGCYC	1 cut(s)	463
Eco31I	GGTCTC	1 cut(s)	1031
Eco47I	GGWCC	1 cut(s)	945
Eco52I	CGGCCG	1 cut(s)	1338
Eco53kI	GAGCTC	1 cut(s)	461
Eco57I	CTGAAG	2 cut(s)	485, 1407
Eco72I	CACGTG	1 cut(s)	201
Eco88I	CYCGRG	1 cut(s)	505
EcoICRI	GAGCTC	1 cut(s)	461
EcoRI	GAATTC	1 cut(s)	1447
EcoRII	CCWGG	1 cut(s)	1020
EcoT14I	CCWWGG	1 cut(s)	266
EcoT38I	GRGCYC	1 cut(s)	463
ErhI	CCWWGG	1 cut(s)	266
FaeI	CATG	7 cut(s)	301, 1133, 1219, 1402, 1414, 1430, 1538
FalI	AAGNNNNNCTT	2 cut(s)	912, 944
FaqI	GGGAC	1 cut(s)	1357
FatI	CATG	7 cut(s)	297, 1129, 1215, 1398, 1410, 1426, 1534
FauNDI	CATATG	1 cut(s)	625
Fnu4HI	GCNGC	4 cut(s)	864, 887, 1403, 1406
FokI	GGATG	3 cut(s)	199, 242, 286
FriOI	GRGCYC	1 cut(s)	463
Fsp4HI	GCNGC	4 cut(s)	864, 887, 1403, 1406
FspBI	CTAG	4 cut(s)	174, 267, 723, 1284
GluI	GCNGC	4 cut(s)	864, 887, 1403, 1406
GsaI	CCCAGC	1 cut(s)	856
GsuI	CTGGAG	1 cut(s)	888
HaeIII	GGCC	1 cut(s)	1340
HapII	CCGG	4 cut(s)	585, 828, 1341, 1364
Hin1II	CATG	7 cut(s)	301, 1133, 1219, 1402, 1414, 1430, 1538
HindIII	AAGCTT	1 cut(s)	80
HinfI	GANTC	6 cut(s)	373, 404, 629, 1071, 1381, 1391
HpaII	CCGG	4 cut(s)	585, 828, 1341, 1364
HphI	GGTGA	1 cut(s)	793
Hpy166II	GTNNAC	2 cut(s)	196, 198
Hpy188I	TCNGA	9 cut(s)	157, 189, 244, 458, 465, 526, 1179, 1523, 1578
Hpy188III	TCNNGA	6 cut(s)	489, 723, 1113, 1364, 1385, 1452
Hpy8I	GTNNAC	2 cut(s)	196, 198
HpyAV	CCTTC	9 cut(s)	84, 104, 444, 668, 705, 814, 1180, 1433, 1518
HpyCH4IV	ACGT	1 cut(s)	200
HpyCH4V	TGCA	7 cut(s)	212, 335, 370, 442, 889, 901, 1497
HpyF10VI	GCNNNNNNNGC	6 cut(s)	111, 863, 915, 1130, 1196, 1284
HpyF3I	CTNAG	7 cut(s)	156, 289, 949, 994, 1160, 1295, 1586
HpySE526I	ACGT	1 cut(s)	200
Hsp92II	CATG	7 cut(s)	301, 1133, 1219, 1402, 1414, 1430, 1538
Kpn2I	TCCGGA	1 cut(s)	1363
KpnI	GGTACC	2 cut(s)	651, 839
Kzo9I	GATC	8 cut(s)	181, 432, 477, 783, 1051, 1140, 1315, 1518
LguI	GCTCTTC	2 cut(s)	591, 703
LmnI	GCTCC	2 cut(s)	458, 936
Lsp1109I	GCAGC	3 cut(s)	873, 875, 1417
LweI	GCATC	3 cut(s)	221, 722, 839
MaeI	CTAG	4 cut(s)	174, 267, 723, 1284
MaeII	ACGT	1 cut(s)	200
MaeIII	GTNAC	5 cut(s)	363, 808, 1005, 1064, 1506
MalI	GATC	8 cut(s)	183, 434, 479, 785, 1053, 1142, 1317, 1520
MboI	GATC	8 cut(s)	181, 432, 477, 783, 1051, 1140, 1315, 1518
MflI	RGATCY	3 cut(s)	181, 432, 477
MhlI	GDGCHC	2 cut(s)	463, 941
MluCI	AATT	8 cut(s)	49, 67, 443, 970, 1146, 1201, 1355, 1447
MlyI	GAGTC	1 cut(s)	1375
MmeI	TCCRAC	2 cut(s)	402, 751
MroI	TCCGGA	1 cut(s)	1363
MseI	TTAA	7 cut(s)	341, 378, 498, 681, 777, 804, 845
MspA1I	CMGCKG	1 cut(s)	866
MspCI	CTTAAG	1 cut(s)	844
MspI	CCGG	4 cut(s)	585, 828, 1341, 1364
MspR9I	CCNGG	2 cut(s)	1022, 1342
Mva1269I	GAATGC	2 cut(s)	1337, 1576
MvaI	CCWGG	1 cut(s)	1022
MwoI	GCNNNNNNNGC	6 cut(s)	111, 863, 915, 1130, 1196, 1284
NciI	CCSGG	1 cut(s)	1342
NdeI	CATATG	1 cut(s)	625
NdeII	GATC	8 cut(s)	181, 432, 477, 783, 1051, 1140, 1315, 1518
NheI	GCTAGC	1 cut(s)	1283
NlaIII	CATG	7 cut(s)	301, 1133, 1219, 1402, 1414, 1430, 1538
NlaIV	GGNNCC	2 cut(s)	649, 837
NmeAIII	GCCGAG	2 cut(s)	1259, 1316
NmuCI	GTSAC	1 cut(s)	1506
NspI	RCATGY	1 cut(s)	1133
PaeR7I	CTCGAG	1 cut(s)	505
PciSI	GCTCTTC	2 cut(s)	591, 703
PctI	GAATGC	2 cut(s)	1337, 1576
PfeI	GAWTC	5 cut(s)	373, 404, 629, 1071, 1391
PinAI	ACCGGT	1 cut(s)	827
PkrI	GCNGC	4 cut(s)	865, 888, 1404, 1407
PleI	GAGTC	1 cut(s)	1375
PmaCI	CACGTG	1 cut(s)	201
PmlI	CACGTG	1 cut(s)	201
PpsI	GAGTC	1 cut(s)	1375
Ppu21I	YACGTR	1 cut(s)	201
Psp124BI	GAGCTC	1 cut(s)	463
Psp6I	CCWGG	1 cut(s)	1020
PspCI	CACGTG	1 cut(s)	201
PspFI	CCCAGC	1 cut(s)	852
PspGI	CCWGG	1 cut(s)	1020
PspN4I	GGNNCC	2 cut(s)	649, 837
PspPI	GGNCC	1 cut(s)	945
PspXI	VCTCGAGB	1 cut(s)	505
PstI	CTGCAG	1 cut(s)	372
PsuI	RGATCY	3 cut(s)	181, 432, 477
PvuII	CAGCTG	1 cut(s)	866
RsaI	GTAC	6 cut(s)	197, 317, 649, 837, 914, 959
RsaNI	GTAC	6 cut(s)	196, 316, 648, 836, 913, 958
SacI	GAGCTC	1 cut(s)	463
SapI	GCTCTTC	2 cut(s)	591, 703
SaqAI	TTAA	7 cut(s)	341, 378, 498, 681, 777, 804, 845
SatI	GCNGC	4 cut(s)	864, 887, 1403, 1406
Sau3AI	GATC	8 cut(s)	181, 432, 477, 783, 1051, 1140, 1315, 1518
Sau96I	GGNCC	1 cut(s)	945
SchI	GAGTC	1 cut(s)	1375
ScrFI	CCNGG	2 cut(s)	1022, 1342
SduI	GDGCHC	2 cut(s)	463, 941
SfaNI	GCATC	3 cut(s)	221, 722, 839
SfcI	CTRYAG	2 cut(s)	368, 1031
Sfr274I	CTCGAG	1 cut(s)	505
SinI	GGWCC	1 cut(s)	945
SlaI	CTCGAG	1 cut(s)	505
SmlI	CTYRAG	5 cut(s)	26, 505, 844, 1511, 1612
SmoI	CTYRAG	5 cut(s)	26, 505, 844, 1511, 1612
Sse9I	AATT	8 cut(s)	49, 67, 443, 970, 1146, 1201, 1355, 1447
SsiI	CCGC	1 cut(s)	1402
SspI	AATATT	1 cut(s)	574
SspMI	CTAG	4 cut(s)	174, 267, 723, 1284
SstI	GAGCTC	1 cut(s)	463
StyD4I	CCNGG	2 cut(s)	1020, 1340
StyI	CCWWGG	1 cut(s)	266
TaiI	ACGT	1 cut(s)	203
TaqI	TCGA	3 cut(s)	506, 967, 1192
TaqII	GACCGA	1 cut(s)	933
TasI	AATT	8 cut(s)	49, 67, 443, 970, 1146, 1201, 1355, 1447
TatI	WGTACW	2 cut(s)	195, 315
TauI	GCSGC	1 cut(s)	1405
TfiI	GAWTC	5 cut(s)	373, 404, 629, 1071, 1391
Tru1I	TTAA	7 cut(s)	341, 378, 498, 681, 777, 804, 845
Tru9I	TTAA	7 cut(s)	341, 378, 498, 681, 777, 804, 845
TscAI	CASTG	2 cut(s)	337, 1637
TseFI	GTSAC	1 cut(s)	1506
TseI	GCWGC	3 cut(s)	863, 886, 1405
Tsp45I	GTSAC	1 cut(s)	1506
TspDTI	ATGAA	9 cut(s)	35, 275, 687, 891, 1232, 1248, 1415, 1427, 1608
TspGWI	ACGGA	2 cut(s)	569, 575
TspRI	CASTG	2 cut(s)	337, 1637
Vha464I	CTTAAG	1 cut(s)	844
VpaK11BI	GGWCC	1 cut(s)	945
XapI	RAATTY	3 cut(s)	1146, 1355, 1447
XbaI	TCTAGA	1 cut(s)	722
XceI	RCATGY	1 cut(s)	1133
XhoI	CTCGAG	1 cut(s)	505
XmaJI	CCTAGG	1 cut(s)	266
XspI	CTAG	4 cut(s)	174, 267, 723, 1284

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.