Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0007140

Physical Location & Seq

Genomic Coordinates Reverse (-)

64312855 .. 64315798

Gene Structure 2944 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G040560.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1506 bp

ATGGGTTTGGGTTTCAGGCTGAACCTCAAGTGTGGTTTTCCATTTCATAATTCTCATTGGCTGACCAATTCTCCTTCCAAGCTTTCTTCGACTTCTGTTAGAGGCAGGGAAGGCTCTTCTTTCAGTTCAGCAAGGCTATCCTTTTTGTCTGTTATTTCTGAGGAATGTGAGATTCCTAATAAAGATTCTTTCAAAGTGTACACGTGTCCATCTGCATCCCCACAACTCTTAGATGATGGTTACTCAGAAGTGGAAGAGAAGATGAACATTAGGGGGATGTCCAACTCCAACAGTGGCTCACCTAACATGATTGATGATAAAGAGCACAGCTTTATATCCAGTGCAGGGTTAAGAACATCTCATTTTAGCTTGTTACTGCAAAATCTTAATGTTTTGGAGGAGACTTTTGCTGATTCAGATGTGTCAAAGTTGGAAAAGGAGATCCTTTTGCAATTAGGAAGGCTTGGAGTTCTGAAGATGAATATTGTCCGTTCTGGCAAAAGGGAAGAACGAAAATTAAGGAAAAGAACATCAGATGATTCCAAAAAGTTTACTGGTTCCTTGCCTTTAAAACCAATGAAGGAAGGTTTTAAAAAGCCTACTCCTTCATCATTTAGAAGAGCACCGAATTCTAGAAGTCGAAGACTATTGTTGGCTAAAAATGAGGCAGAAATGTCAGTCGGAGTTAAGGAAACTGGTAAAGTACCGACCTTAAGATGCTGGGCAGAAGCAGCTGGAGTTGATGAAAAGGTGCTGCAACAACAATTGCATTTCGGTTGGTATTGCCAAGATGAGCTTGTAAGGAGCAGTCGGTCCTTGGTTCTATACCTTGCTCGAAACTATAGAGGACTTGGAGTAGCCTTGGATGATTTGCTGCAGGCTGGAAACCTGGGACTCCTACAAGGTGCAGAAAGATTTGATCCCTTGCGGGGTTACAGATTCTCGACCTATGTCCAATACTGGATAAGAAAATCAATGTCAAGATTGCTGGCACAACATGCCAGAGGGATCAAAATTCCTAACACACTAAGTAAAGCAATAAATCGGATACAGAAGGCTCGAAAAGCCAATTACAGCAGCCACATGAAATACCCAGATGATGAAGAAATTGCAAAGATTACAGGTCTTTCTTTGGCGAAAATCAGATCGGCCAGCAGGTGTCTGAGAGTTGTGGGTTCAGTAGATCAGAAGATATGGGAATGCTCAGCTGGGACGTATATGGAGTTTACTCCAGATATAGCAGTAGAGAGTCCTGAAGAACCTGTCATGCGGCAACACATGAAAAACGACATTCATGATCTGCTGGAAGGCTTGAATTCTAGAGAGAAGCAAGTTTTGGCACTACGATATGGTCTAAACTGCTGCAACCCCAAGTCACTTGAGGAGATTGGAAGGCTTTTCCATGTGAGCAAGGAGTGGATACGAAAGATAGAAAAGAATGCTCTGACCAAGCTGAGGGATGAAGAAACCAACAAAAACTTGAGCCATTATTTGAAGCAGTGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000988 GO:0000990 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006351 GO:0006352 GO:0006355 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009416 GO:0009507 GO:0009536 GO:0009628 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0016987 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031326 GO:0032774 GO:0034062 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043254 GO:0044087 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051252 GO:0051716 GO:0060255 GO:0065007 GO:0071214 GO:0071478 GO:0071482 GO:0071704 GO:0080090 GO:0090304 GO:0097659 GO:0097747 GO:0104004 GO:0140098 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1903506 GO:2000112 GO:2000142 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Sigma70_r2 Sigma70_r3 Sigma70_r4

Protein Analysis

501

Amino Acids

56.87

Weight (kDa)

9.51

Isoelectric Point (pI)

50.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Sigma70_r2	PF04542	266 - 335	3.2e-15	Sigma-70 region 2
Sigma70_r3	PF04539	346 - 420	6.9e-09	Sigma-70 region 3
Sigma70_r4	PF04545	434 - 486	2.7e-14	Sigma-70, region 4

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0007140)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G53920 AT3G53920
fragaria_vesca	FvH4_2g35110 FvH4_2g35110 FvH4_2g35110
malus_domestica	MD08G1108600.v1.1 MD15G1089100.v1.1
prunus_persica	Prupe.1G443200_v2.0.a1 Prupe.1G443200_v2.0.a1 Prupe.1G443200_v2.0.a1
pyrus_communis	pycom08g09050 pycom15g08350
rosa_chinensis	RchiOBHm_Chr6g0306861
rosa_laevigata	RLG00000010760
rosa_multiflora	Rmu_sc0009381.1_g000005
rosa_roxburghii	Rroxscaffold_7G00161410
rosa_rugosa	Rorug06G0353600 Rorug06G0353700 Rorug06G0353800 Rorug06G0353900
rosa_samantha	Rh6AG465400 Rh6BG435400 Rh6CG479800 Rh6DG466800
rosa_wichuraiana	Rw6G040560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1146
Acc36I	ACCTGC	1 cut(s)	1146
AciI	CCGC	2 cut(s)	928, 1270
AclWI	GGATC	3 cut(s)	436, 914, 1016
AcoI	YGGCCR	1 cut(s)	1149
AcsI	RAATTY	3 cut(s)	628, 1014, 1315
AcuI	CTGAAG	2 cut(s)	494, 1275
AcvI	CACGTG	1 cut(s)	204
AfaI	GTAC	2 cut(s)	200, 705
AfiI	CCNNNNNNNGG	3 cut(s)	345, 929, 1455
AflII	CTTAAG	1 cut(s)	712
AflIII	ACRYGT	2 cut(s)	201, 203
AgsI	TTSAA	3 cut(s)	193, 1315, 1495
AjnI	CCWGG	1 cut(s)	888
AjuI	GAANNNNNNNTTGG	2 cut(s)	1319, 1351
AluBI	AGCT	7 cut(s)	82, 330, 369, 734, 796, 1208, 1453
AluI	AGCT	7 cut(s)	82, 330, 369, 734, 796, 1208, 1453
Alw21I	GWGCWC	2 cut(s)	327, 625
Alw26I	GTCTC	1 cut(s)	395
AlwI	GGATC	3 cut(s)	436, 914, 1016
AoxI	GGCC	1 cut(s)	1149
ApeKI	GCWGC	5 cut(s)	731, 754, 874, 1077, 1362
ApoI	RAATTY	3 cut(s)	628, 1014, 1315
AspS9I	GGNCC	1 cut(s)	813
AsuHPI	GGTGA	1 cut(s)	291
AvaII	GGWCC	1 cut(s)	813
BbrPI	CACGTG	1 cut(s)	204
BbsI	GAAGAC	1 cut(s)	649
Bbv12I	GWGCWC	2 cut(s)	327, 625
BbvCI	CCTCAGC	1 cut(s)	1454
BbvI	GCAGC	5 cut(s)	741, 743, 861, 1089, 1349
BccI	CCATC	2 cut(s)	217, 230
BciT130I	CCWGG	1 cut(s)	890
BciVI	GTATCC	2 cut(s)	1041, 1413
BcoDI	GTCTC	1 cut(s)	395
BfaI	CTAG	2 cut(s)	633, 1320
BfmI	CTRYAG	2 cut(s)	841, 875
BfrI	CTTAAG	1 cut(s)	712
BfuAI	ACCTGC	1 cut(s)	1146
BfuI	GTATCC	2 cut(s)	1041, 1413
BisI	GCNGC	6 cut(s)	732, 755, 875, 1078, 1271, 1363
BlpI	GCTNAGC	1 cut(s)	1204
BlsI	GCNGC	6 cut(s)	733, 756, 876, 1079, 1272, 1364
Bme1390I	CCNGG	1 cut(s)	890
Bme18I	GGWCC	1 cut(s)	813
BmgT120I	GGNCC	1 cut(s)	813
BmiI	GGNNCC	1 cut(s)	559
BmrFI	CCNGG	1 cut(s)	890
BmsI	GCATC	2 cut(s)	224, 707
BoxI	GACNNNNGTC	1 cut(s)	950
BpiI	GAAGAC	1 cut(s)	649
BpmI	CTGGAG	2 cut(s)	756, 1215
Bpu10I	CCTNAGC	1 cut(s)	1454
Bpu1102I	GCTNAGC	1 cut(s)	1204
BpuEI	CTTGAG	3 cut(s)	11, 1400, 1501
BsaAI	YACGTR	1 cut(s)	204
BsaJI	CCNNGG	3 cut(s)	816, 861, 889
BsaXI	ACNNNNNCTCC	2 cut(s)	55, 85
Bsc4I	CCNNNNNNNGG	3 cut(s)	345, 929, 1455
Bse1I	ACTGG	4 cut(s)	339, 559, 700, 965
BseBI	CCWGG	1 cut(s)	890
BseDI	CCNNGG	3 cut(s)	816, 861, 889
BseGI	GGATG	4 cut(s)	215, 282, 871, 1465
BseLI	CCNNNNNNNGG	3 cut(s)	345, 929, 1455
BseMII	CTCAG	5 cut(s)	150, 258, 1154, 1218, 1445
BseNI	ACTGG	4 cut(s)	339, 559, 700, 965
BseRI	GAGGAG	2 cut(s)	413, 1397
BseXI	GCAGC	5 cut(s)	741, 743, 861, 1089, 1349
BseYI	CCCAGC	2 cut(s)	720, 1208
BsgI	GTGCAG	2 cut(s)	363, 927
BshFI	GGCC	1 cut(s)	1151
BsiHKAI	GWGCWC	2 cut(s)	327, 625
BslFI	GGGAC	2 cut(s)	906, 1225
BslI	CCNNNNNNNGG	3 cut(s)	345, 929, 1455
BsmAI	GTCTC	1 cut(s)	395
BsmFI	GGGAC	2 cut(s)	906, 1225
BsmI	GAATGC	2 cut(s)	1205, 1444
BsnI	GGCC	1 cut(s)	1151
Bsp1286I	GDGCHC	2 cut(s)	327, 625
Bsp1407I	TGTACA	1 cut(s)	198
Bsp143I	GATC	6 cut(s)	441, 919, 1008, 1145, 1183, 1297
Bsp1720I	GCTNAGC	1 cut(s)	1204
BspACI	CCGC	2 cut(s)	928, 1270
BspANI	GGCC	1 cut(s)	1151
BspCNI	CTCAG	5 cut(s)	151, 257, 1155, 1217, 1446
BspHI	TCATGA	1 cut(s)	1294
BspLI	GGNNCC	1 cut(s)	559
BspMAI	CTGCAG	1 cut(s)	879
BspMI	ACCTGC	1 cut(s)	1146
BspPI	GGATC	3 cut(s)	436, 914, 1016
BspQI	GCTCTTC	2 cut(s)	121, 613
BspTI	CTTAAG	1 cut(s)	712
BsrGI	TGTACA	1 cut(s)	198
BsrI	ACTGG	4 cut(s)	339, 559, 700, 965
BssECI	CCNNGG	3 cut(s)	816, 861, 889
BssMI	GATC	6 cut(s)	441, 919, 1008, 1145, 1183, 1297
BssT1I	CCWWGG	2 cut(s)	816, 861
Bst2UI	CCWGG	1 cut(s)	890
Bst4CI	ACNGT	1 cut(s)	293
Bst6I	CTCTTC	3 cut(s)	121, 249, 613
BstAFI	CTTAAG	1 cut(s)	712
BstAPI	GCANNNNNTGC	1 cut(s)	998
BstAUI	TGTACA	1 cut(s)	198
BstBAI	YACGTR	1 cut(s)	204
BstC8I	GCNNGC	3 cut(s)	879, 990, 1153
BstDEI	CTNAG	7 cut(s)	159, 229, 244, 1028, 1163, 1204, 1454
BstF5I	GGATG	4 cut(s)	215, 282, 871, 1465
BstKTI	GATC	6 cut(s)	444, 922, 1011, 1148, 1186, 1300
BstMAI	GTCTC	1 cut(s)	395
BstMBI	GATC	6 cut(s)	441, 919, 1008, 1145, 1183, 1297
BstMWI	GCNNNNNNNGC	4 cut(s)	111, 731, 998, 1064
BstNI	CCWGG	1 cut(s)	890
BstNSI	RCATGY	1 cut(s)	1001
BstPAI	GACNNNNGTC	1 cut(s)	950
BstSCI	CCNGG	1 cut(s)	888
BstSFI	CTRYAG	2 cut(s)	841, 875
BstV1I	GCAGC	5 cut(s)	741, 743, 861, 1089, 1349
BstV2I	GAAGAC	1 cut(s)	649
BstX2I	RGATCY	1 cut(s)	441
BstYI	RGATCY	1 cut(s)	441
BsuI	GTATCC	2 cut(s)	1041, 1413
BsuRI	GGCC	1 cut(s)	1151
BtsCI	GGATG	4 cut(s)	215, 282, 871, 1465
BtsI	GCAGTG	1 cut(s)	1505
BtsIMutI	CAGTG	3 cut(s)	298, 346, 1505
BveI	ACCTGC	1 cut(s)	1146
Cac8I	GCNNGC	3 cut(s)	879, 990, 1153
CciI	TCATGA	1 cut(s)	1294
Cfr13I	GGNCC	1 cut(s)	813
Csp6I	GTAC	2 cut(s)	199, 704
CviAII	CATG	7 cut(s)	307, 998, 1084, 1267, 1279, 1295, 1403
CviQI	GTAC	2 cut(s)	199, 704
DdeI	CTNAG	7 cut(s)	159, 229, 244, 1028, 1163, 1204, 1454
DpnI	GATC	6 cut(s)	443, 921, 1010, 1147, 1185, 1299
DpnII	GATC	6 cut(s)	441, 919, 1008, 1145, 1183, 1297
DraI	TTTAAA	2 cut(s)	570, 592
EaeI	YGGCCR	1 cut(s)	1149
Eam1104I	CTCTTC	3 cut(s)	121, 249, 613
EarI	CTCTTC	3 cut(s)	121, 249, 613
Eco130I	CCWWGG	2 cut(s)	816, 861
Eco47I	GGWCC	1 cut(s)	813
Eco57I	CTGAAG	2 cut(s)	494, 1275
Eco72I	CACGTG	1 cut(s)	204
EcoRI	GAATTC	2 cut(s)	628, 1315
EcoRII	CCWGG	1 cut(s)	888
EcoT14I	CCWWGG	2 cut(s)	816, 861
ErhI	CCWWGG	2 cut(s)	816, 861
FaeI	CATG	7 cut(s)	310, 1001, 1087, 1270, 1282, 1298, 1406
FalI	AAGNNNNNCTT	2 cut(s)	780, 812
FaqI	GGGAC	2 cut(s)	906, 1225
FatI	CATG	7 cut(s)	306, 997, 1083, 1266, 1278, 1294, 1402
FauI	CCCGC	1 cut(s)	921
Fnu4HI	GCNGC	6 cut(s)	732, 755, 875, 1078, 1271, 1363
FokI	GGATG	4 cut(s)	202, 289, 878, 1472
Fsp4HI	GCNGC	6 cut(s)	732, 755, 875, 1078, 1271, 1363
FspBI	CTAG	2 cut(s)	633, 1320
GluI	GCNGC	6 cut(s)	732, 755, 875, 1078, 1271, 1363
GsaI	CCCAGC	2 cut(s)	724, 1212
GsuI	CTGGAG	2 cut(s)	756, 1215
HaeIII	GGCC	1 cut(s)	1151
Hin1II	CATG	7 cut(s)	310, 1001, 1087, 1270, 1282, 1298, 1406
HindIII	AAGCTT	1 cut(s)	80
HinfI	GANTC	7 cut(s)	172, 185, 413, 539, 894, 939, 1249
HphI	GGTGA	1 cut(s)	291
Hpy166II	GTNNAC	4 cut(s)	199, 201, 552, 1227
Hpy188III	TCNNGA	7 cut(s)	633, 943, 981, 1232, 1253, 1295, 1320
Hpy8I	GTNNAC	4 cut(s)	199, 201, 552, 1227
HpyAV	CCTTC	9 cut(s)	84, 104, 453, 574, 578, 615, 1048, 1301, 1386
HpyCH4III	ACNGT	1 cut(s)	293
HpyCH4IV	ACGT	2 cut(s)	203, 1214
HpyF10VI	GCNNNNNNNGC	4 cut(s)	111, 731, 998, 1064
HpyF3I	CTNAG	7 cut(s)	159, 229, 244, 1028, 1163, 1204, 1454
HpySE526I	ACGT	2 cut(s)	203, 1214
Hsp92II	CATG	7 cut(s)	310, 1001, 1087, 1270, 1282, 1298, 1406
Kzo9I	GATC	6 cut(s)	441, 919, 1008, 1145, 1183, 1297
LguI	GCTCTTC	2 cut(s)	121, 613
LmnI	GCTCC	1 cut(s)	804
Lsp1109I	GCAGC	5 cut(s)	741, 743, 861, 1089, 1349
LweI	GCATC	2 cut(s)	224, 707
MaeI	CTAG	2 cut(s)	633, 1320
MaeII	ACGT	2 cut(s)	203, 1214
MaeIII	GTNAC	4 cut(s)	239, 372, 932, 1374
MalI	GATC	6 cut(s)	443, 921, 1010, 1147, 1185, 1299
MboI	GATC	6 cut(s)	441, 919, 1008, 1145, 1183, 1297
MfeI	CAATTG	1 cut(s)	764
MflI	RGATCY	1 cut(s)	441
MhlI	GDGCHC	2 cut(s)	327, 625
MlyI	GAGTC	2 cut(s)	888, 1258
MmeI	TCCRAC	4 cut(s)	306, 312, 411, 661
MnlI	CCTC	9 cut(s)	35, 95, 154, 391, 658, 839, 998, 1375, 1449
MseI	TTAA	7 cut(s)	350, 387, 518, 569, 591, 687, 713
MspA1I	CMGCKG	2 cut(s)	734, 1208
MspCI	CTTAAG	1 cut(s)	712
MspR9I	CCNGG	1 cut(s)	890
MunI	CAATTG	1 cut(s)	764
Mva1269I	GAATGC	2 cut(s)	1205, 1444
MvaI	CCWGG	1 cut(s)	890
MwoI	GCNNNNNNNGC	4 cut(s)	111, 731, 998, 1064
NdeII	GATC	6 cut(s)	441, 919, 1008, 1145, 1183, 1297
NlaIII	CATG	7 cut(s)	310, 1001, 1087, 1270, 1282, 1298, 1406
NlaIV	GGNNCC	1 cut(s)	559
NmuCI	GTSAC	1 cut(s)	1374
NspI	RCATGY	1 cut(s)	1001
PagI	TCATGA	1 cut(s)	1294
PaqCI	CACCTGC	1 cut(s)	1146
PciSI	GCTCTTC	2 cut(s)	121, 613
PctI	GAATGC	2 cut(s)	1205, 1444
PfeI	GAWTC	5 cut(s)	172, 185, 413, 539, 939
PkrI	GCNGC	6 cut(s)	733, 756, 876, 1079, 1272, 1364
PleI	GAGTC	2 cut(s)	888, 1257
PmaCI	CACGTG	1 cut(s)	204
PmlI	CACGTG	1 cut(s)	204
PpsI	GAGTC	2 cut(s)	888, 1257
Ppu21I	YACGTR	1 cut(s)	204
PshAI	GACNNNNGTC	1 cut(s)	950
Psp6I	CCWGG	1 cut(s)	888
PspCI	CACGTG	1 cut(s)	204
PspFI	CCCAGC	2 cut(s)	720, 1208
PspGI	CCWGG	1 cut(s)	888
PspN4I	GGNNCC	1 cut(s)	559
PspPI	GGNCC	1 cut(s)	813
PstI	CTGCAG	1 cut(s)	879
PsuI	RGATCY	1 cut(s)	441
PvuII	CAGCTG	2 cut(s)	734, 1208
RsaI	GTAC	2 cut(s)	200, 705
RsaNI	GTAC	2 cut(s)	199, 704
SapI	GCTCTTC	2 cut(s)	121, 613
SaqAI	TTAA	7 cut(s)	350, 387, 518, 569, 591, 687, 713
SatI	GCNGC	6 cut(s)	732, 755, 875, 1078, 1271, 1363
Sau3AI	GATC	6 cut(s)	441, 919, 1008, 1145, 1183, 1297
Sau96I	GGNCC	1 cut(s)	813
SchI	GAGTC	2 cut(s)	888, 1258
ScrFI	CCNGG	1 cut(s)	890
SduI	GDGCHC	2 cut(s)	327, 625
SfaNI	GCATC	2 cut(s)	224, 707
SfcI	CTRYAG	2 cut(s)	841, 875
SinI	GGWCC	1 cut(s)	813
SmlI	CTYRAG	4 cut(s)	26, 712, 1379, 1480
SmoI	CTYRAG	4 cut(s)	26, 712, 1379, 1480
SsiI	CCGC	2 cut(s)	928, 1270
SspI	AATATT	1 cut(s)	484
SspMI	CTAG	2 cut(s)	633, 1320
StyD4I	CCNGG	1 cut(s)	888
StyI	CCWWGG	2 cut(s)	816, 861
TaaI	ACNGT	1 cut(s)	293
TaiI	ACGT	2 cut(s)	206, 1217
TaqI	TCGA	5 cut(s)	89, 640, 835, 944, 1060
TaqII	GACCGA	1 cut(s)	801
TatI	WGTACW	1 cut(s)	198
TauI	GCSGC	1 cut(s)	1273
TfiI	GAWTC	5 cut(s)	172, 185, 413, 539, 939
Tru1I	TTAA	7 cut(s)	350, 387, 518, 569, 591, 687, 713
Tru9I	TTAA	7 cut(s)	350, 387, 518, 569, 591, 687, 713
TscAI	CASTG	3 cut(s)	298, 346, 1505
TseFI	GTSAC	1 cut(s)	1374
TseI	GCWGC	5 cut(s)	731, 754, 874, 1077, 1362
Tsp45I	GTSAC	1 cut(s)	1374
TspGWI	ACGGA	1 cut(s)	479
TspRI	CASTG	3 cut(s)	298, 346, 1505
Vha464I	CTTAAG	1 cut(s)	712
VpaK11BI	GGWCC	1 cut(s)	813
XapI	RAATTY	3 cut(s)	628, 1014, 1315
XbaI	TCTAGA	2 cut(s)	632, 1319
XceI	RCATGY	1 cut(s)	1001
XspI	CTAG	2 cut(s)	633, 1320

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.