FvH4_2g37120

Histidine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0009350
Physical Location & Seq
Forward (+)
27074091 .. 27079715
5625 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g37120.t2

Sequence Viewer

Length: 3030 bp
ATGGTTTGTGAAATGGAGATCGTTAATATTGAAGAAATGGCAATGGAGATGGAGGAAGTTCTGCCTTCTATGTGGCCTGAGGATATAGATTCCGATAAGCAATTCAACATCGAAAAGCCAGGACAAGATCAAGACATGTTGGAGGAGGTAACATTGAAAGAGAAACCGACTAACGTGGATTTCAAGCGGCTCTTTGAGCTAACCAAGAACACAGATACAGGCTCTTCCCAATTGGCAAACCTTGTAAAGCACTGGGAATACAAGCAGGCGAATGTCGTGCGCCTTCTGCAAGAAGAACTTGACATACTCAGCAAGCAAAAACAGGAAGTTGAGCTCAAGAAATTAGAGATATTGGAAGAGCACCGTTTCGAGTCAGAGAACTACGGCGGTGACAAACGCCCCATTTCCATTTTGGACGAAGTGTATCAGTTATGGGCGCCAGATGTTCCTCTAAAGAAGCTTGATGTTGTTGTGGAGAGTAAGAGAGTCGAGACAGAAGCCGAGTACGACAGTGTTGTGTACTGGAAACAAAGGGCTATGAATTTGGAGAAGTTGTTGGAGGCAAGTGTTGTGAGAGAGCAGATTCTGGTCGAACAATTGCAGGAAAGCATTAAGAGTATTGAAAGACAGTCATCGCCGGTTGAAGAATTGTCTGAGATTCTGAAAAGAGCAGATAATTTCTTGCATTTTGTACTTCAAAATGCACCTGTTGTTATTGGTCATCAGGATAAAGAATTGCGGTATCGCTTTATCTACAATCACTTCCCAAGTCTGGGAGAGCAAGATATTATAGGGAAAACAGATGTTGAGATTTTCACTGGGGCTGGAGTTAAGGAATCTCAAGACTTCAAGAGAGAGGTTCTGGAAAAAGGGATACCGGCGAAAAGGGAGATTACATTTGAGACTGAATTATTTGGTTCAAAGACGTTTCTGATATATGTGGAACCTGTTTTCAACAAGGCAGGGGAGACAATTGGTATAAATTATATGGGGATGGAGGTGTCTGATCAGGTGAGGAAAAGAGAGAAGATGGCAAAGCTTCGTGAGGAAATAGCTGTCCAAAAGGCCAAGGAAACAGAGCTAAACAAGACAATTCACATAACGGAGGAGACAATGCGAGCAAAACAGATGCTGGCAACAATGTCTCATGAGATACGGTCTCCTCTTTCTGGGGTTGTCAGCATGGCCGAGATTCTTACCACCACGAAACTTGACCGCGAGCAACGGCAGCTGTTAGAAGTCATGTTATCTTCAGGAGATTTGGTCCTCCAACTGATAAATGACATCCTTGACCTTTCCAAGGTTGAGTCAGGAGTGATGAAGTTGGAGGCTACAAAGTTCCGGCCAAGACAGGTGGTTAAGCATGTACTACAGACTGCGGCAGCATCATTACAGAAAGTTTTGACCTTGGAAGGACATATTATGGAGGAAGTTCCTGTGGAGGTCATTGGAGATGTTTTAAGGATTCGTCAAATCCTAACCAACTTGATCAGTAATGCAATCAAGTTTACTCATGAAGGAAAAGTTGGGATTAAACTTTACATGGTAGAGGATCCAACTTCTGGATGTCTGCAGACGCCGGATGCAGTTCGACCAACGGTTTCAGTGAATGGATTGAATGAAGAGAAATCAGCATCACGAAATAGTTGTAATGGAGAAGTCTTTTGTGGGAACGAACCTTACACAAATGGTTGTATTCATGATGAACCTAGCACACCAGTTAAGAGCGAGGCCTCAATGAATGGAGATAAAGAAGGGGAACCTCATTCATCTGAAACAAAAACAGTGTGGTTACGATGTGATGTGTATGACACAGGAATTGGAATACCAGAAAATGCTTTGCCGACCCTTTTTAAAAGATACATGCAAGTGAGTGCTGATCATGCTCGAAAATATGGCGGGACAGGATTAGGACTAGCAATATGCAAACAATTGGTTGAACTAATGGGGGGACAACTCACCGTGTCTAGCCGAGTAAACTGTGGCTCAACATTCACATTCGTATTACCTTACAAGGTTTCAACATCGTCTGAAAATTCTGATGATCCTGATGAGTTAACGGATATGGATGATGCTGTAACTGATGATGAAGTAGCTGAGAGCTACTTCCAGTTCCAACCACGCACTTTGGGTTCTCTGTTCTCTTCTAATAGCACCAGCAGGACTCAAAAACTGCTACCACATAAGCTCAATGGATTGTCACAAAATGCATACTCGTTTCCATATAATAACATCAGGCCAGAGGAGACAGCTTCAGTTGAAAATTGCTGTTCAACAGTTGATGATGTCGCTGAGACATTATCAGAACCAGAGAGTTCAGTGAGTCACACCTCAAATTCTAAATGCAAAACTCCAGCTGGTAGAGACAGAAAAGGTCAAGATGACTCAAACAGTCAATCTCAAAATTCTTCATCAGATTCAAATCAACATATAGAGGCAAGTAGACACAATGGTGTTGTAGCAAAGACAAGAGAACCACAAGGAACGTGTAAGAGACAAGAGAAACCAGATGCAGACTCCGAATGTAGCTCTGGCAACAGTTCGAAGATACCTAAATCAACCTCAAAACCTAAGATCCTTCTTGTTGAAGATAACAAGATCAACATCATGGTGGCACAGTCGATGATGAAGCAGTTAGGCCAAAGCATAGAGGTTGTGAACAACGGAGCTGAAGCTGTCCGCGCAGTTCAGCGCTATAGATATGATCTCATTCTTATGGATGTTCATATGCCAGTTATGGACGGTCTACAAGCTACAAGAATAATACGCTCTTTCGAAGAAACTGGCAACTGGGAAGCTGCAGAGAAAGCTGGAATTGAGCAATCTGTGCCTTGTTCAAATACACTACAAAACGGCCATGGACCTTGTGGAAAGCGAATCCCAATCATTGCGATGACAGCAAATGCATTTTCAGAGAGCGCAGAGGAGTGTTTTGCAAATGGGATGGACTCGTTTGTGCCCAAACCAGTAACGTGTCAAAAACTGAAAGAGAGGCTTGAACAATATCTCCCAGATTGCAACCTAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000302 GO:0002237 GO:0003674 GO:0003824 GO:0004672 GO:0004673 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009617 GO:0009636 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009755 GO:0009787 GO:0009788 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010104 GO:0010105 GO:0010119 GO:0010646 GO:0010648 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016775 GO:0018106 GO:0018193 GO:0018202 GO:0019538 GO:0022622 GO:0023051 GO:0023052 GO:0023057 GO:0032501 GO:0032502 GO:0032870 GO:0033554 GO:0034599 GO:0034614 GO:0035690 GO:0036211 GO:0042221 GO:0042493 GO:0042542 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046677 GO:0048364 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0070297 GO:0070298 GO:0070301 GO:0070887 GO:0071216 GO:0071219 GO:0071236 GO:0071241 GO:0071310 GO:0071368 GO:0071495 GO:0071704 GO:0071731 GO:0071732 GO:0071944 GO:0090333 GO:0097237 GO:0097366 GO:0099402 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902170 GO:1902531 GO:1902532 GO:1905957 GO:1905958
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HATPase_c HisKA Response_reg

Protein Analysis

1010

Amino Acids

113.16

Weight (kDa)

5.05

Isoelectric Point (pI)

52.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HisKA PF00512 375 - 438 1.7e-18 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 595 - 671 5.2e-20 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Response_reg PF00072 859 - 926 2e-14 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 436
AccB7I CCANNNNNTGG 1 cut(s) 1562
AccI GTMKAC 2 cut(s) 2443, 2746
AccII CGCG 2 cut(s) 1218, 2682
AciI CCGC 7 cut(s) 187, 387, 739, 1216, 1379, 1899, 2680
AclWI GGATC 4 cut(s) 1547, 1560, 2039, 2569
AcoI YGGCCR 3 cut(s) 1185, 1343, 2854
AcsI RAATTY 4 cut(s) 541, 2035, 2335, 2404
AcuI CTGAAG 3 cut(s) 1236, 2238, 2691
AcyI GRCGYC 2 cut(s) 437, 1577
AfaI GTAC 4 cut(s) 506, 521, 693, 1368
AfeI AGCGCT 1 cut(s) 2693
AfiI CCNNNNNNNGG 5 cut(s) 772, 773, 1169, 1300, 1562
AflIII ACRYGT 3 cut(s) 135, 2486, 2972
AjnI CCWGG 1 cut(s) 118
AjuI GAANNNNNNNTTGG 2 cut(s) 1509, 1541
AleI CACNNNNGTG 1 cut(s) 2451
AloI GAACNNNNNNTCC 2 cut(s) 2991, 3023
Alw21I GWGCWC 2 cut(s) 336, 363
AlwI GGATC 4 cut(s) 1547, 1560, 2039, 2569
AlwNI CAGNNNCTG 2 cut(s) 586, 1132
Aor51HI AGCGCT 1 cut(s) 2693
AoxI GGCC 8 cut(s) 74, 1065, 1185, 1343, 1731, 2237, 2638, 2854
ApeKI GCWGC 3 cut(s) 1228, 1382, 2798
ApoI RAATTY 4 cut(s) 541, 2035, 2335, 2404
AspLEI GCGC 5 cut(s) 282, 439, 2684, 2694, 2921
AspS9I GGNCC 2 cut(s) 1264, 2861
AsuHPI GGTGA 3 cut(s) 401, 1024, 1951
AsuII TTCGAA 2 cut(s) 2543, 2775
AvaII GGWCC 2 cut(s) 1264, 2861
AxyI CCTNAGG 1 cut(s) 78
BaeGI GKGCMC 1 cut(s) 2961
BamHI GGATCC 1 cut(s) 1552
BanI GGYRCC 1 cut(s) 436
BanII GRGCYC 1 cut(s) 336
Bbv12I GWGCWC 2 cut(s) 336, 363
BbvI GCAGC 3 cut(s) 1240, 1394, 2785
BccI CCATC 4 cut(s) 43, 988, 1024, 2938
BceAI ACGGC 3 cut(s) 400, 1241, 2869
BcgI CGANNNNNNTGC 2 cut(s) 259, 293
BciT130I CCWGG 1 cut(s) 120
BciVI GTATCC 1 cut(s) 867
BclI TGATCA 3 cut(s) 1006, 1488, 1879
BfaI CTAG 4 cut(s) 1710, 1916, 1968, 3023
BfmI CTRYAG 4 cut(s) 1370, 1571, 2695, 2799
BfoI RGCGCY 2 cut(s) 440, 2695
BfuI GTATCC 1 cut(s) 867
BisI GCNGC 5 cut(s) 188, 1229, 1380, 1383, 2799
BlsI GCNGC 5 cut(s) 189, 1230, 1381, 1384, 2800
Bme1390I CCNGG 1 cut(s) 120
Bme18I GGWCC 2 cut(s) 1264, 2861
BmgT120I GGNCC 2 cut(s) 1264, 2861
BmiI GGNNCC 4 cut(s) 438, 945, 1554, 1761
BmrFI CCNGG 1 cut(s) 120
BmrI ACTGGG 3 cut(s) 262, 828, 2800
BmsI GCATC 6 cut(s) 1119, 1394, 1573, 1643, 2062, 2500
BmuI ACTGGG 3 cut(s) 262, 828, 2800
BpmI CTGGAG 2 cut(s) 846, 2337
Bpu14I TTCGAA 2 cut(s) 2543, 2775
BpuEI CTTGAG 2 cut(s) 320, 825
BsaBI GATNNNNATC 2 cut(s) 2421, 2603
BsaHI GRCGYC 2 cut(s) 437, 1577
BsaI GGTCTC 1 cut(s) 1164
BsaJI CCNNGG 4 cut(s) 1068, 1299, 1407, 2857
BsaXI ACNNNNNCTCC 6 cut(s) 539, 569, 1248, 1278, 2991, 3021
Bsc4I CCNNNNNNNGG 5 cut(s) 772, 773, 1169, 1300, 1562
Bse118I RCCGGY 2 cut(s) 637, 877
Bse1I ACTGG 9 cut(s) 257, 527, 823, 1718, 2110, 2732, 2788, 2795, 2966
Bse21I CCTNAGG 1 cut(s) 78
Bse3DI GCAATG 2 cut(s) 48, 2886
Bse8I GATNNNNATC 2 cut(s) 2421, 2603
BseBI CCWGG 1 cut(s) 120
BseDI CCNNGG 4 cut(s) 1068, 1299, 1407, 2857
BseGI GGATG 7 cut(s) 999, 1284, 1571, 1588, 2074, 2725, 2949
BseJI GATNNNNATC 2 cut(s) 2421, 2603
BseLI CCNNNNNNNGG 5 cut(s) 772, 773, 1169, 1300, 1562
BseMI GCAATG 2 cut(s) 48, 2886
BseMII CTCAG 5 cut(s) 69, 322, 645, 2088, 2283
BseNI ACTGG 9 cut(s) 257, 527, 823, 1718, 2110, 2732, 2788, 2795, 2966
BseRI GAGGAG 5 cut(s) 158, 1121, 1152, 2258, 2939
BseSI GKGCMC 1 cut(s) 2961
BseXI GCAGC 3 cut(s) 1240, 1394, 2785
Bsh1236I CGCG 2 cut(s) 1218, 2682
BshFI GGCC 8 cut(s) 76, 1067, 1187, 1345, 1733, 2239, 2640, 2856
BshNI GGYRCC 1 cut(s) 436
BsiHKAI GWGCWC 2 cut(s) 336, 363
BsiSI CCGG 4 cut(s) 638, 878, 1342, 1580
BslFI GGGAC 2 cut(s) 1915, 1965
BslI CCNNNNNNNGG 5 cut(s) 772, 773, 1169, 1300, 1562
BsmFI GGGAC 2 cut(s) 1915, 1965
BsnI GGCC 8 cut(s) 76, 1067, 1187, 1345, 1733, 2239, 2640, 2856
Bso31I GGTCTC 1 cut(s) 1164
Bsp119I TTCGAA 2 cut(s) 2543, 2775
Bsp1286I GDGCHC 3 cut(s) 336, 363, 2961
Bsp19I CCATGG 1 cut(s) 2857
BspACI CCGC 7 cut(s) 187, 387, 739, 1216, 1379, 1899, 2680
BspANI GGCC 8 cut(s) 76, 1067, 1187, 1345, 1733, 2239, 2640, 2856
BspCNI CTCAG 5 cut(s) 70, 321, 646, 2089, 2284
BspFNI CGCG 2 cut(s) 1218, 2682
BspHI TCATGA 3 cut(s) 1147, 1513, 1699
BspLI GGNNCC 4 cut(s) 438, 945, 1554, 1761
BspMAI CTGCAG 2 cut(s) 1575, 2803
BspPI GGATC 4 cut(s) 1547, 1560, 2039, 2569
BspQI GCTCTTC 2 cut(s) 229, 351
BspT104I TTCGAA 2 cut(s) 2543, 2775
BspT107I GGYRCC 1 cut(s) 436
BspTNI GGTCTC 1 cut(s) 1164
BsrDI GCAATG 2 cut(s) 48, 2886
BsrFI RCCGGY 2 cut(s) 637, 877
BsrI ACTGG 9 cut(s) 257, 527, 823, 1718, 2110, 2732, 2788, 2795, 2966
BssAI RCCGGY 2 cut(s) 637, 877
BssECI CCNNGG 4 cut(s) 1068, 1299, 1407, 2857
BssNI GRCGYC 2 cut(s) 437, 1577
BssT1I CCWWGG 4 cut(s) 1068, 1299, 1407, 2857
Bst2UI CCWGG 1 cut(s) 120
Bst6I CTCTTC 4 cut(s) 229, 351, 1617, 2149
BstACI GRCGYC 2 cut(s) 437, 1577
BstAPI GCANNNNNTGC 1 cut(s) 2827
BstBI TTCGAA 2 cut(s) 2543, 2775
BstC8I GCNNGC 5 cut(s) 267, 314, 1119, 1134, 1220
BstDEI CTNAG 6 cut(s) 78, 308, 654, 2097, 2292, 2571
BstDSI CCRYGG 1 cut(s) 2857
BstENI CCTNNNNNAGG 1 cut(s) 1298
BstF5I GGATG 7 cut(s) 999, 1284, 1571, 1588, 2074, 2725, 2949
BstFNI CGCG 2 cut(s) 1218, 2682
BstH2I RGCGCY 2 cut(s) 440, 2695
BstHHI GCGC 5 cut(s) 282, 439, 2684, 2694, 2921
BstMWI GCNNNNNNNGC 8 cut(s) 196, 286, 1228, 1883, 2681, 2807, 2827, 2897
BstNI CCWGG 1 cut(s) 120
BstNSI RCATGY 3 cut(s) 139, 1367, 1867
BstSCI CCNGG 1 cut(s) 118
BstSFI CTRYAG 4 cut(s) 1370, 1571, 2695, 2799
BstSLI GKGCMC 1 cut(s) 2961
BstUI CGCG 2 cut(s) 1218, 2682
BstV1I GCAGC 3 cut(s) 1240, 1394, 2785
BstX2I RGATCY 2 cut(s) 1552, 2574
BstYI RGATCY 2 cut(s) 1552, 2574
Bsu36I CCTNAGG 1 cut(s) 78
BsuI GTATCC 1 cut(s) 867
BsuRI GGCC 8 cut(s) 76, 1067, 1187, 1345, 1733, 2239, 2640, 2856
BtgI CCRYGG 1 cut(s) 2857
BtgZI GCGATG 2 cut(s) 618, 2906
BtsCI GGATG 7 cut(s) 999, 1284, 1571, 1588, 2074, 2725, 2949
BtsIMutI CAGTG 6 cut(s) 250, 517, 816, 1611, 1791, 2325
Cac8I GCNNGC 5 cut(s) 267, 314, 1119, 1134, 1220
CaiI CAGNNNCTG 2 cut(s) 586, 1132
CciI TCATGA 3 cut(s) 1147, 1513, 1699
CfoI GCGC 5 cut(s) 282, 439, 2684, 2694, 2921
Cfr10I RCCGGY 2 cut(s) 637, 877
Cfr13I GGNCC 2 cut(s) 1264, 2861
CseI GACGC 1 cut(s) 1585
Csp6I GTAC 4 cut(s) 505, 520, 692, 1367
CspCI CAANNNNNGTGG 6 cut(s) 1192, 1227, 1335, 1370, 2109, 2144
CviQI GTAC 4 cut(s) 505, 520, 692, 1367
DdeI CTNAG 6 cut(s) 78, 308, 654, 2097, 2292, 2571
DinI GGCGCC 1 cut(s) 438
DraI TTTAAA 1 cut(s) 1855
EaeI YGGCCR 3 cut(s) 1185, 1343, 2854
Eam1104I CTCTTC 4 cut(s) 229, 351, 1617, 2149
EarI CTCTTC 4 cut(s) 229, 351, 1617, 2149
Ecl136II GAGCTC 1 cut(s) 334
Eco130I CCWWGG 4 cut(s) 1068, 1299, 1407, 2857
Eco147I AGGCCT 1 cut(s) 1733
Eco24I GRGCYC 1 cut(s) 336
Eco31I GGTCTC 1 cut(s) 1164
Eco47I GGWCC 2 cut(s) 1264, 2861
Eco47III AGCGCT 1 cut(s) 2693
Eco53kI GAGCTC 1 cut(s) 334
Eco57I CTGAAG 3 cut(s) 1236, 2238, 2691
Eco81I CCTNAGG 1 cut(s) 78
EcoICRI GAGCTC 1 cut(s) 334
EcoNI CCTNNNNNAGG 1 cut(s) 1298
EcoRII CCWGG 1 cut(s) 118
EcoT14I CCWWGG 4 cut(s) 1068, 1299, 1407, 2857
EcoT22I ATGCAT 2 cut(s) 2212, 2908
EcoT38I GRGCYC 1 cut(s) 336
EgeI GGCGCC 1 cut(s) 438
EheI GGCGCC 1 cut(s) 438
ErhI CCWWGG 4 cut(s) 1068, 1299, 1407, 2857
FalI AAGNNNNNCTT 6 cut(s) 176, 208, 282, 314, 2979, 3011
FaqI GGGAC 2 cut(s) 1915, 1965
FauI CCCGC 1 cut(s) 1892
FauNDI CATATG 1 cut(s) 2727
FbaI TGATCA 3 cut(s) 1006, 1488, 1879
FblI GTMKAC 2 cut(s) 2443, 2746
Fnu4HI GCNGC 5 cut(s) 188, 1229, 1380, 1383, 2799
FokI GGATG 7 cut(s) 1006, 1271, 1578, 1595, 2081, 2732, 2956
FriOI GRGCYC 1 cut(s) 336
Fsp4HI GCNGC 5 cut(s) 188, 1229, 1380, 1383, 2799
FspBI CTAG 4 cut(s) 1710, 1916, 1968, 3023
GlaI GCGC 5 cut(s) 281, 438, 2683, 2693, 2920
GluI GCNGC 5 cut(s) 188, 1229, 1380, 1383, 2799
GsuI CTGGAG 2 cut(s) 846, 2337
HaeII RGCGCY 2 cut(s) 440, 2695
HaeIII GGCC 8 cut(s) 76, 1067, 1187, 1345, 1733, 2239, 2640, 2856
HapII CCGG 4 cut(s) 638, 878, 1342, 1580
HgaI GACGC 1 cut(s) 1585
HhaI GCGC 5 cut(s) 282, 439, 2684, 2694, 2921
Hin1I GRCGYC 2 cut(s) 437, 1577
Hin6I GCGC 5 cut(s) 280, 437, 2682, 2692, 2919
HinP1I GCGC 5 cut(s) 280, 437, 2682, 2692, 2919
HincII GTYRAC 1 cut(s) 2058
HindII GTYRAC 1 cut(s) 2058
HindIII AAGCTT 2 cut(s) 458, 1037
HpaI GTTAAC 1 cut(s) 2058
HpaII CCGG 4 cut(s) 638, 878, 1342, 1580
HphI GGTGA 3 cut(s) 401, 1024, 1951
Hpy166II GTNNAC 7 cut(s) 520, 1509, 1978, 2058, 2444, 2659, 2747
Hpy8I GTNNAC 7 cut(s) 520, 1509, 1978, 2058, 2444, 2659, 2747
HpyAV CCTTC 6 cut(s) 75, 293, 1406, 1511, 1748, 2588
HpyCH4IV ACGT 4 cut(s) 174, 926, 2486, 2972
HpyF10VI GCNNNNNNNGC 8 cut(s) 196, 286, 1228, 1883, 2681, 2807, 2827, 2897
HpyF3I CTNAG 6 cut(s) 78, 308, 654, 2097, 2292, 2571
HpySE526I ACGT 4 cut(s) 174, 926, 2486, 2972
Hsp92I GRCGYC 2 cut(s) 437, 1577
HspAI GCGC 5 cut(s) 280, 437, 2682, 2692, 2919
KasI GGCGCC 1 cut(s) 436
Ksp22I TGATCA 3 cut(s) 1006, 1488, 1879
KspAI GTTAAC 1 cut(s) 2058
LguI GCTCTTC 2 cut(s) 229, 351
LmnI GCTCC 1 cut(s) 2666
Lsp1109I GCAGC 3 cut(s) 1240, 1394, 2785
LweI GCATC 6 cut(s) 1119, 1394, 1573, 1643, 2062, 2500
MaeI CTAG 4 cut(s) 1710, 1916, 1968, 3023
MaeII ACGT 4 cut(s) 174, 926, 2486, 2972
MaeIII GTNAC 7 cut(s) 148, 389, 1791, 2077, 2199, 2324, 2968
MfeI CAATTG 4 cut(s) 230, 596, 972, 1931
MflI RGATCY 2 cut(s) 1552, 2574
MhlI GDGCHC 3 cut(s) 336, 363, 2961
Mly113I GGCGCC 1 cut(s) 437
MlyI GAGTC 8 cut(s) 380, 495, 1316, 2158, 2332, 2378, 2510, 2942
MmeI TCCRAC 6 cut(s) 120, 537, 1294, 1305, 1580, 2140
Mph1103I ATGCAT 2 cut(s) 2212, 2908
MseI TTAA 9 cut(s) 24, 612, 831, 1359, 1460, 1533, 1722, 1854, 2057
MslI CAYNNNNRTG 5 cut(s) 1868, 2451, 2609, 2714, 2891
MspA1I CMGCKG 2 cut(s) 1231, 2357
MspI CCGG 4 cut(s) 638, 878, 1342, 1580
MspR9I CCNGG 1 cut(s) 120
MunI CAATTG 4 cut(s) 230, 596, 972, 1931
MvaI CCWGG 1 cut(s) 120
MvnI CGCG 2 cut(s) 1218, 2682
MwoI GCNNNNNNNGC 8 cut(s) 196, 286, 1228, 1883, 2681, 2807, 2827, 2897
NarI GGCGCC 1 cut(s) 437
NcoI CCATGG 1 cut(s) 2857
NdeI CATATG 1 cut(s) 2727
NlaIV GGNNCC 4 cut(s) 438, 945, 1554, 1761
NmeAIII GCCGAG 3 cut(s) 526, 1213, 1997
NmuCI GTSAC 3 cut(s) 389, 2199, 2324
NsiI ATGCAT 2 cut(s) 2212, 2908
NspI RCATGY 3 cut(s) 139, 1367, 1867
NspV TTCGAA 2 cut(s) 2543, 2775
OliI CACNNNNGTG 1 cut(s) 2451
PagI TCATGA 3 cut(s) 1147, 1513, 1699
PceI AGGCCT 1 cut(s) 1733
PciI ACATGT 1 cut(s) 135
PciSI GCTCTTC 2 cut(s) 229, 351
PfeI GAWTC 8 cut(s) 89, 583, 658, 836, 1192, 1465, 2417, 2877
PflMI CCANNNNNTGG 1 cut(s) 1562
PkrI GCNGC 5 cut(s) 189, 1230, 1381, 1384, 2800
PleI GAGTC 8 cut(s) 379, 494, 1315, 2158, 2331, 2378, 2510, 2942
PluTI GGCGCC 1 cut(s) 440
PpsI GAGTC 8 cut(s) 379, 494, 1315, 2158, 2331, 2378, 2510, 2942
PscI ACATGT 1 cut(s) 135
Psp124BI GAGCTC 1 cut(s) 336
Psp6I CCWGG 1 cut(s) 118
PspGI CCWGG 1 cut(s) 118
PspN4I GGNNCC 4 cut(s) 438, 945, 1554, 1761
PspPI GGNCC 2 cut(s) 1264, 2861
PsrI GAACNNNNNNTAC 4 cut(s) 288, 320, 1664, 1696
PstI CTGCAG 2 cut(s) 1575, 2803
PstNI CAGNNNCTG 2 cut(s) 586, 1132
PsuI RGATCY 2 cut(s) 1552, 2574
PvuII CAGCTG 2 cut(s) 1231, 2357
RsaI GTAC 4 cut(s) 506, 521, 693, 1368
RsaNI GTAC 4 cut(s) 505, 520, 692, 1367
RseI CAYNNNNRTG 5 cut(s) 1868, 2451, 2609, 2714, 2891
SacI GAGCTC 1 cut(s) 336
SapI GCTCTTC 2 cut(s) 229, 351
SaqAI TTAA 9 cut(s) 24, 612, 831, 1359, 1460, 1533, 1722, 1854, 2057
SatI GCNGC 5 cut(s) 188, 1229, 1380, 1383, 2799
Sau96I GGNCC 2 cut(s) 1264, 2861
SchI GAGTC 8 cut(s) 380, 495, 1316, 2158, 2332, 2378, 2510, 2942
ScrFI CCNGG 1 cut(s) 120
SduI GDGCHC 3 cut(s) 336, 363, 2961
SfaNI GCATC 6 cut(s) 1119, 1394, 1573, 1643, 2062, 2500
SfcI CTRYAG 4 cut(s) 1370, 1571, 2695, 2799
SfoI GGCGCC 1 cut(s) 438
SfuI TTCGAA 2 cut(s) 2543, 2775
SinI GGWCC 2 cut(s) 1264, 2861
SmiMI CAYNNNNRTG 5 cut(s) 1868, 2451, 2609, 2714, 2891
SmlI CTYRAG 2 cut(s) 335, 840
SmoI CTYRAG 2 cut(s) 335, 840
SseBI AGGCCT 1 cut(s) 1733
SsiI CCGC 7 cut(s) 187, 387, 739, 1216, 1379, 1899, 2680
SspDI GGCGCC 1 cut(s) 436
SspI AATATT 1 cut(s) 28
SspMI CTAG 4 cut(s) 1710, 1916, 1968, 3023
SstI GAGCTC 1 cut(s) 336
StuI AGGCCT 1 cut(s) 1733
StyD4I CCNGG 1 cut(s) 118
StyI CCWWGG 4 cut(s) 1068, 1299, 1407, 2857
TaiI ACGT 4 cut(s) 177, 929, 2489, 2975
TaqI TCGA 9 cut(s) 111, 369, 489, 591, 1591, 1888, 2543, 2621, 2775
TatI WGTACW 3 cut(s) 519, 691, 1366
TauI GCSGC 2 cut(s) 190, 1382
TfiI GAWTC 8 cut(s) 89, 583, 658, 836, 1192, 1465, 2417, 2877
Tru1I TTAA 9 cut(s) 24, 612, 831, 1359, 1460, 1533, 1722, 1854, 2057
Tru9I TTAA 9 cut(s) 24, 612, 831, 1359, 1460, 1533, 1722, 1854, 2057
TscAI CASTG 6 cut(s) 257, 517, 823, 1611, 1791, 2325
TseFI GTSAC 3 cut(s) 389, 2199, 2324
TseI GCWGC 3 cut(s) 1228, 1382, 2798
Tsp45I GTSAC 3 cut(s) 389, 2199, 2324
TspGWI ACGGA 3 cut(s) 1118, 2075, 2679
TspRI CASTG 6 cut(s) 257, 517, 823, 1611, 1791, 2325
Van91I CCANNNNNTGG 1 cut(s) 1562
VpaK11BI GGWCC 2 cut(s) 1264, 2861
XagI CCTNNNNNAGG 1 cut(s) 1298
XapI RAATTY 4 cut(s) 541, 2035, 2335, 2404
XceI RCATGY 3 cut(s) 139, 1367, 1867
XcmI CCANNNNNNNNNTGG 2 cut(s) 409, 2864
XmiI GTMKAC 2 cut(s) 2443, 2746
XspI CTAG 4 cut(s) 1710, 1916, 1968, 3023
Zsp2I ATGCAT 2 cut(s) 2212, 2908
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.