Rroxscaffold_7G00163610

Histidine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0009350
Physical Location & Seq
Forward (+)
5797893 .. 5803091
5199 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00163610.1

Sequence Viewer

Length: 3066 bp
ATGGTTTGTGAAATGGAGATCGATAATATTGAAGAAATGGAAATAGAGGAAGTCCTCCCTTCTATGTGGCCTGAGGATATAGATTCCGATAAGCAATTCAACATTGAAAAGCCAGGAAAAGATCAAGACATGTTGGAAGAGGTCACATTCAAAGAGAAACCAACTACCGTCGATTTCAGGCGGCTGTTGGAGCTAACCAAACACACAGATACAGGCTCTTCCCAATTAGCACACCTCGTAAAGCACTGGGAGTATAAGCAGGCGAATGTGGTGAGACTTCTTAGAGAAGAACTTGATATCCTCAGCAAGCAAAAGCAGGAAGTTGAGCTCAAGAAATTGGAGATATTGGAAGAGCACCGCTTCGAGTCAGAGTGCTATGGCGGTGACAAACGCCCCGTTTCTATTTTGGATGAAGTGTATCAGTTATGGGCTCCGGATGTTCCTCTAAAGAAGCTTGATGTTGTTGTGGAGAGTAAGAGAGTCGAGACAGAAGCCGAGTATGACAGTGTTGTGTACTGGAAGCAAAGGGTTATGCATTTGGAGAAGTTGTTGGAGGCAAGTGTTGCGAGAGAGCAGATTCTTGTCGATAAATTGCAGGAAAGCATTAAGAGTATTGAAAGACAGTCATCGCCGGTTGATGAGCTGTCTGAGATTTTGAAAAGAGCAGACAACTTCCTGCATTTTGTTCTTCAGAATGCCCCTGTTGTTATTGGTCATCAGGATAAAGAGTTGCGGTATCGCTTTATCTACAATCACTTCCCAAGTTTGGGAGAGCAGGACATTATAGGGAGAACAGATGTCGAGATTTTCAGTGGGGCTGGAGTTAAGGAATCTCAAGACTTCAAGAGAGAGGTTCTGGAAAAAGGGATACCGGCGAAAAGGGAGATTACATTCGAGACTGAATTGTTTGGGTCAAAGACGTTTTTGATATATGTGGAACCTGTTTTCAACAAGGCAGGGGAGACAATTGGTATAAACTATATGGGGATGGAGGTGTCTGATCAGGTGAGGAAAAGAGAGAAGATGGCCAAGCTTCGTGAGGAAATAGCCGTCCAAAAGGCCAAGGAAACAGAGCTGAACAAGACAATTCACATAACGGAGGAGACAATGAGAGCAAAACAGATGCTGGCAACAATGTCTCATGAGATAAGGTCTCCTCTTTCTGGGGTTGTCAGCATGGCTGAGATTCTTACCACCACGAAACTTGACCGTGAGCAACGCCAGCTGTTGGAAGTCATGTTATCTTCAGGAGATTTGGTCCTCCAACTGATAAATGACATCCTTGACCTTTCCAAGGTTGAGTCAGGAGTAATGAAGTTGGAGGCTACAAAGTTCCGGCCAAGACAGGTAGTTAGACATGTACTACAGACTGCGGCAGCATCATTGCAGAAAATTTTGACCTTGGAAGGACATGTTATGGAGAATGTTCCTGTGGAGGTCATTGGAGATGTTTTAAGGATTCGTCAAATTCTAACCAACTTGATCAGTAATGCAATCAAGTTTACTCATGAAGGGAAAGTCGGGATAAAACTTTACATGGTAGCGGATCCAACTTCTAGAGAAGATGAAAGATGTCTGCAGGTGTCAAATGCAGTTCAATCAACGGTTTCAGTGAATGGATTGAATGAAGAGAAATCAGCATCACAAAATTGCTGCAATGGAGAAGGTATTGGTCGCAAAGAACCTTACCAAAATGGTTCTCTTCACAATGAACCTAGCACACCAGTTAAGAGCACACCGGTTAAGAGTGAAGCCTCAATGAATGGAGATAAAGAGGAGGAACCCCATTCATCTGAAACAACAACAGTCTGGTTACGCTGCGATGTATATGACACAGGAATTGGAATACCAGAAAATGCTTTGCCGACCCTTTTTAAAAGATACATGCAAGTGAGTGCTGATCATGCTCGAAAATATGGCGGGACAGGATTAGGACTAGCAATATGCAAACAATTGGTTGAACTAATGGGGGGCCAACTCACTGTGTCTAGCCGAGAACATTGTGGCTCCACATTCACATTTGTATTACCTTACAAGGTTTCAACATCGTCGGAACATTCTGATGATCCTGATGAGCTCACGGATATCGCTGAATATGACGATGCTGTAACTGATGATGAAGTAGCTGAAAGCTATTTCCAGTTCCAACCACGCACTTTAGGTTCTCTGTTCTCTTCTAATGGCACCGGCAGGACTCAAAAACTGTTACCACATAAACTCAATGGATTATCACAACATGCATACTCGTTTCCATATAATAACATCAGACCAGAGGAGATTACTTCAGTTGAAAATTGCTGTTCTGCAGTCGATGATGTTGCTGAGACATTATCTGAACCAGAGAGTTTAGTAAGTCACATGCCGAATTCCAATAGCGAAACTCCAGCGTGTAGGGACAGAAAGGGTCAAGATGACTCAAACAGTCAATCCCAAAATTCTTCAACAGATTCAAATCATCATGCAGAGGCAAGCAGAGAAAATGGTGTAGCAGCAAAGACAAGAGAACCTCAAGGAACGTGTAATAGACAGGAGAAATCAGATGCAGATTCAGAGTGTAGCTCTGGCAACAGTCCAAAAATACCAAAATCAACATCGAATCCTAAGATTCTTCTTGTTGAAGATAACAAGATCAATATTATGGTGGCACAGTCAATGATGAAGCAGTTAGGCCAAAGCTTAGAGGTTGTAAATGACGGAGCTGAAGCTGTGCGTGCAGTTCAACGCTGTAGTTACGATCTGATTCTGATGGATGTTCATATGCCAGTTATGGATGGCCTACAAGCTACAAGAATAATACGTTCTTTCGAAGAAACTGGCAACTGGGAAGCTGCTAAGAAAGCTGGAATTGAGCAATCTGTGCCTTCTTCAAATACACTACAAAACGGCCACGGACATACGCCTTGTAGAAAGCGAATCCCAATCATTGCGATGACAGCAAATGCATTTGCAGAGAGCGCAGAAGAGTGTTTTGCAAATGGTATGGACTCGTTTGTGCCGAAACCTGTAACGCGCCAAAAATTGAAAGAGCGGCTTGAACAATATCTACCCGATTGCAACCTAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000302 GO:0002237 GO:0003674 GO:0003824 GO:0004672 GO:0004673 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009617 GO:0009636 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009755 GO:0009787 GO:0009788 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010104 GO:0010105 GO:0010119 GO:0010646 GO:0010648 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016775 GO:0018106 GO:0018193 GO:0018202 GO:0019538 GO:0022622 GO:0023051 GO:0023052 GO:0023057 GO:0032501 GO:0032502 GO:0032870 GO:0033554 GO:0034599 GO:0034614 GO:0035690 GO:0036211 GO:0042221 GO:0042493 GO:0042542 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046677 GO:0048364 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0070297 GO:0070298 GO:0070301 GO:0070887 GO:0071216 GO:0071219 GO:0071236 GO:0071241 GO:0071310 GO:0071368 GO:0071495 GO:0071704 GO:0071731 GO:0071732 GO:0071944 GO:0090333 GO:0097237 GO:0097366 GO:0099402 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902170 GO:1902531 GO:1902532 GO:1905957 GO:1905958
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HATPase_c HisKA Response_reg

Protein Analysis

1021

Amino Acids

114.73

Weight (kDa)

5.14

Isoelectric Point (pI)

53.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HisKA PF00512 373 - 436 1.7e-18 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 602 - 678 2.9e-20 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Response_reg PF00072 869 - 936 3.8e-13 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1570
Acc36I ACCTGC 1 cut(s) 1570
AccB1I GGYRCC 1 cut(s) 2183
AccB7I CCANNNNNTGG 1 cut(s) 1228
AccBSI CCGCTC 1 cut(s) 3028
AccII CGCG 1 cut(s) 3010
AccIII TCCGGA 1 cut(s) 433
AciI CCGC 8 cut(s) 181, 358, 381, 733, 1373, 1544, 1920, 3028
AclWI GGATC 3 cut(s) 1541, 1554, 2060
AcoI YGGCCR 3 cut(s) 1026, 1337, 2884
AcsI RAATTY 4 cut(s) 1392, 1467, 2365, 2434
AcuI CTGAAG 4 cut(s) 674, 1230, 2268, 2721
AfaI GTAC 2 cut(s) 515, 1362
AfiI CCNNNNNNNGG 5 cut(s) 766, 767, 1163, 1228, 1294
AflIII ACRYGT 4 cut(s) 129, 1357, 1411, 2516
AgeI ACCGGT 1 cut(s) 1738
AjnI CCWGG 1 cut(s) 112
AloI GAACNNNNNNTCC 2 cut(s) 282, 314
Alw21I GWGCWC 4 cut(s) 330, 357, 1736, 2079
Alw26I GTCTC 8 cut(s) 268, 479, 890, 956, 1097, 1143, 1158, 2318
AlwI GGATC 3 cut(s) 1541, 1554, 2060
AlwNI CAGNNNCTG 1 cut(s) 1126
Aor13HI TCCGGA 1 cut(s) 433
AoxI GGCC 8 cut(s) 68, 1026, 1059, 1337, 1972, 2668, 2773, 2884
ApeKI GCWGC 5 cut(s) 1376, 1653, 1818, 2489, 2828
ApoI RAATTY 4 cut(s) 1392, 1467, 2365, 2434
AsiGI ACCGGT 1 cut(s) 1738
AspLEI GCGC 2 cut(s) 2957, 3012
AspS9I GGNCC 2 cut(s) 1258, 1972
AsuHPI GGTGA 3 cut(s) 283, 395, 1018
AsuII TTCGAA 1 cut(s) 2805
AvaII GGWCC 1 cut(s) 1258
AxyI CCTNAGG 1 cut(s) 72
BalI TGGCCA 1 cut(s) 1028
BamHI GGATCC 1 cut(s) 1546
BanI GGYRCC 1 cut(s) 2183
BanII GRGCYC 3 cut(s) 330, 433, 2079
Bbv12I GWGCWC 4 cut(s) 330, 357, 1736, 2079
BbvCI CCTCAGC 1 cut(s) 302
BbvI GCAGC 5 cut(s) 1388, 1640, 1805, 2501, 2815
BccI CCATC 4 cut(s) 982, 1018, 2740, 2765
BceAI ACGGC 2 cut(s) 1034, 2899
BcgI CGANNNNNNTGC 2 cut(s) 2300, 2334
BciT130I CCWGG 1 cut(s) 114
BciVI GTATCC 1 cut(s) 861
BclI TGATCA 3 cut(s) 1000, 1482, 1900
BcoDI GTCTC 8 cut(s) 268, 479, 890, 956, 1097, 1143, 1158, 2318
BfaI CTAG 5 cut(s) 1557, 1716, 1937, 1989, 3059
BfmI CTRYAG 4 cut(s) 1364, 1577, 2304, 2725
BfuAI ACCTGC 1 cut(s) 1570
BfuI GTATCC 1 cut(s) 861
BisI GCNGC 8 cut(s) 182, 1374, 1377, 1654, 1819, 2490, 2829, 3029
BlsI GCNGC 8 cut(s) 183, 1375, 1378, 1655, 1820, 2491, 2830, 3030
Bme1390I CCNGG 1 cut(s) 114
Bme18I GGWCC 1 cut(s) 1258
BmgT120I GGNCC 2 cut(s) 1258, 1972
BmiI GGNNCC 7 cut(s) 432, 939, 1548, 1782, 1973, 2008, 2185
BmrFI CCNGG 1 cut(s) 114
BmrI ACTGGG 2 cut(s) 256, 2830
BmsI GCATC 5 cut(s) 1113, 1388, 1649, 2092, 2530
BmuI ACTGGG 2 cut(s) 256, 2830
BplI GAGNNNNNCTC 2 cut(s) 2543, 2575
BpmI CTGGAG 2 cut(s) 840, 2367
Bpu10I CCTNAGC 1 cut(s) 302
Bpu14I TTCGAA 1 cut(s) 2805
BpuEI CTTGAG 3 cut(s) 314, 819, 2493
Bsa29I ATCGAT 1 cut(s) 21
BsaBI GATNNNNATC 1 cut(s) 2451
BsaI GGTCTC 1 cut(s) 1158
BsaJI CCNNGG 4 cut(s) 1062, 1293, 1401, 2887
BsaWI WCCGGW 2 cut(s) 433, 1738
BsaXI ACNNNNNCTCC 4 cut(s) 533, 563, 1242, 1272
Bsc4I CCNNNNNNNGG 5 cut(s) 766, 767, 1163, 1228, 1294
Bse118I RCCGGY 4 cut(s) 631, 871, 1738, 2186
Bse1I ACTGG 7 cut(s) 251, 521, 1724, 2140, 2762, 2818, 2825
Bse21I CCTNAGG 1 cut(s) 72
Bse3DI GCAATG 3 cut(s) 1382, 1663, 2922
Bse8I GATNNNNATC 1 cut(s) 2451
BseAI TCCGGA 1 cut(s) 433
BseBI CCWGG 1 cut(s) 114
BseCI ATCGAT 1 cut(s) 21
BseDI CCNNGG 4 cut(s) 1062, 1293, 1401, 2887
BseGI GGATG 6 cut(s) 415, 442, 993, 1278, 2755, 2776
BseJI GATNNNNATC 1 cut(s) 2451
BseLI CCNNNNNNNGG 5 cut(s) 766, 767, 1163, 1228, 1294
BseMI GCAATG 3 cut(s) 1382, 1663, 2922
BseMII CTCAG 5 cut(s) 63, 316, 639, 1173, 2313
BseNI ACTGG 7 cut(s) 251, 521, 1724, 2140, 2762, 2818, 2825
BseRI GAGGAG 4 cut(s) 1115, 1146, 1790, 2288
BseXI GCAGC 5 cut(s) 1388, 1640, 1805, 2501, 2815
BsgI GTGCAG 1 cut(s) 2733
Bsh1236I CGCG 1 cut(s) 3010
BshFI GGCC 8 cut(s) 70, 1028, 1061, 1339, 1974, 2670, 2775, 2886
BshNI GGYRCC 1 cut(s) 2183
BshTI ACCGGT 1 cut(s) 1738
BshVI ATCGAT 1 cut(s) 21
BsiHKAI GWGCWC 4 cut(s) 330, 357, 1736, 2079
BsiSI CCGG 6 cut(s) 434, 632, 872, 1336, 1739, 2187
BslFI GGGAC 2 cut(s) 1936, 2408
BslI CCNNNNNNNGG 5 cut(s) 766, 767, 1163, 1228, 1294
BsmAI GTCTC 8 cut(s) 268, 479, 890, 956, 1097, 1143, 1158, 2318
BsmFI GGGAC 2 cut(s) 1936, 2408
BsmI GAATGC 1 cut(s) 700
BsnI GGCC 8 cut(s) 70, 1028, 1061, 1339, 1974, 2670, 2775, 2886
Bso31I GGTCTC 1 cut(s) 1158
Bsp119I TTCGAA 1 cut(s) 2805
Bsp1286I GDGCHC 5 cut(s) 330, 357, 433, 1736, 2079
Bsp13I TCCGGA 1 cut(s) 433
Bsp143I GATC 9 cut(s) 18, 121, 1000, 1482, 1546, 1900, 2065, 2628, 2734
BspACI CCGC 8 cut(s) 181, 358, 381, 733, 1373, 1544, 1920, 3028
BspANI GGCC 8 cut(s) 70, 1028, 1061, 1339, 1974, 2670, 2775, 2886
BspCNI CTCAG 5 cut(s) 64, 315, 640, 1174, 2314
BspDI ATCGAT 1 cut(s) 21
BspEI TCCGGA 1 cut(s) 433
BspFNI CGCG 1 cut(s) 3010
BspHI TCATGA 2 cut(s) 1141, 1507
BspLI GGNNCC 7 cut(s) 432, 939, 1548, 1782, 1973, 2008, 2185
BspMAI CTGCAG 2 cut(s) 1581, 2308
BspMI ACCTGC 1 cut(s) 1570
BspPI GGATC 3 cut(s) 1541, 1554, 2060
BspQI GCTCTTC 2 cut(s) 223, 345
BspT104I TTCGAA 1 cut(s) 2805
BspT107I GGYRCC 1 cut(s) 2183
BspTNI GGTCTC 1 cut(s) 1158
BsrBI CCGCTC 1 cut(s) 3028
BsrDI GCAATG 3 cut(s) 1382, 1663, 2922
BsrFI RCCGGY 4 cut(s) 631, 871, 1738, 2186
BsrI ACTGG 7 cut(s) 251, 521, 1724, 2140, 2762, 2818, 2825
BssAI RCCGGY 4 cut(s) 631, 871, 1738, 2186
BssECI CCNNGG 4 cut(s) 1062, 1293, 1401, 2887
BssMI GATC 9 cut(s) 18, 121, 1000, 1482, 1546, 1900, 2065, 2628, 2734
BssT1I CCWWGG 3 cut(s) 1062, 1293, 1401
Bst2UI CCWGG 1 cut(s) 114
Bst6I CTCTTC 7 cut(s) 132, 223, 345, 1623, 1707, 2179, 2955
BstAPI GCANNNNNTGC 2 cut(s) 563, 2857
BstBI TTCGAA 1 cut(s) 2805
BstC8I GCNNGC 6 cut(s) 261, 308, 1128, 1223, 2470, 2712
BstDEI CTNAG 9 cut(s) 72, 281, 302, 648, 1182, 2322, 2601, 2677, 2832
BstDSI CCRYGG 1 cut(s) 2887
BstENI CCTNNNNNAGG 1 cut(s) 1292
BstF5I GGATG 6 cut(s) 415, 442, 993, 1278, 2755, 2776
BstFNI CGCG 1 cut(s) 3010
BstHHI GCGC 2 cut(s) 2957, 3012
BstKTI GATC 9 cut(s) 21, 124, 1003, 1485, 1549, 1903, 2068, 2631, 2737
BstMAI GTCTC 8 cut(s) 268, 479, 890, 956, 1097, 1143, 1158, 2318
BstMBI GATC 9 cut(s) 18, 121, 1000, 1482, 1546, 1900, 2065, 2628, 2734
BstMWI GCNNNNNNNGC 9 cut(s) 190, 563, 1222, 1904, 2711, 2837, 2857, 2933, 2954
BstNI CCWGG 1 cut(s) 114
BstNSI RCATGY 6 cut(s) 133, 1361, 1415, 1888, 2240, 2362
BstSCI CCNGG 1 cut(s) 112
BstSFI CTRYAG 4 cut(s) 1364, 1577, 2304, 2725
BstUI CGCG 1 cut(s) 3010
BstV1I GCAGC 5 cut(s) 1388, 1640, 1805, 2501, 2815
BstX2I RGATCY 1 cut(s) 1546
BstYI RGATCY 1 cut(s) 1546
Bsu15I ATCGAT 1 cut(s) 21
Bsu36I CCTNAGG 1 cut(s) 72
BsuI GTATCC 1 cut(s) 861
BsuRI GGCC 8 cut(s) 70, 1028, 1061, 1339, 1974, 2670, 2775, 2886
BsuTUI ATCGAT 1 cut(s) 21
BtgI CCRYGG 1 cut(s) 2887
BtgZI GCGATG 3 cut(s) 612, 1836, 2942
BtsCI GGATG 6 cut(s) 415, 442, 993, 1278, 2755, 2776
BtsIMutI CAGTG 5 cut(s) 244, 511, 817, 1617, 1980
BveI ACCTGC 1 cut(s) 1570
Cac8I GCNNGC 6 cut(s) 261, 308, 1128, 1223, 2470, 2712
CaiI CAGNNNCTG 1 cut(s) 1126
CciI TCATGA 2 cut(s) 1141, 1507
CfoI GCGC 2 cut(s) 2957, 3012
Cfr10I RCCGGY 4 cut(s) 631, 871, 1738, 2186
Cfr13I GGNCC 2 cut(s) 1258, 1972
ClaI ATCGAT 1 cut(s) 21
Csp6I GTAC 2 cut(s) 514, 1361
CspAI ACCGGT 1 cut(s) 1738
CspCI CAANNNNNGTGG 2 cut(s) 1186, 1221
CviQI GTAC 2 cut(s) 514, 1361
DdeI CTNAG 9 cut(s) 72, 281, 302, 648, 1182, 2322, 2601, 2677, 2832
DpnI GATC 9 cut(s) 20, 123, 1002, 1484, 1548, 1902, 2067, 2630, 2736
DpnII GATC 9 cut(s) 18, 121, 1000, 1482, 1546, 1900, 2065, 2628, 2734
DraI TTTAAA 1 cut(s) 1876
EaeI YGGCCR 3 cut(s) 1026, 1337, 2884
Eam1104I CTCTTC 7 cut(s) 132, 223, 345, 1623, 1707, 2179, 2955
EarI CTCTTC 7 cut(s) 132, 223, 345, 1623, 1707, 2179, 2955
Ecl136II GAGCTC 2 cut(s) 328, 2077
Eco130I CCWWGG 3 cut(s) 1062, 1293, 1401
Eco24I GRGCYC 3 cut(s) 330, 433, 2079
Eco31I GGTCTC 1 cut(s) 1158
Eco32I GATATC 2 cut(s) 298, 2086
Eco47I GGWCC 1 cut(s) 1258
Eco53kI GAGCTC 2 cut(s) 328, 2077
Eco57I CTGAAG 4 cut(s) 674, 1230, 2268, 2721
Eco81I CCTNAGG 1 cut(s) 72
EcoICRI GAGCTC 2 cut(s) 328, 2077
EcoNI CCTNNNNNAGG 1 cut(s) 1292
EcoRI GAATTC 1 cut(s) 2365
EcoRII CCWGG 1 cut(s) 112
EcoRV GATATC 2 cut(s) 298, 2086
EcoT14I CCWWGG 3 cut(s) 1062, 1293, 1401
EcoT22I ATGCAT 3 cut(s) 537, 2242, 2944
EcoT38I GRGCYC 3 cut(s) 330, 433, 2079
ErhI CCWWGG 3 cut(s) 1062, 1293, 1401
FalI AAGNNNNNCTT 2 cut(s) 3015, 3047
FaqI GGGAC 2 cut(s) 1936, 2408
FauI CCCGC 1 cut(s) 1913
FauNDI CATATG 1 cut(s) 2757
FbaI TGATCA 3 cut(s) 1000, 1482, 1900
Fnu4HI GCNGC 8 cut(s) 182, 1374, 1377, 1654, 1819, 2490, 2829, 3029
FokI GGATG 6 cut(s) 422, 449, 1000, 1265, 2762, 2783
FriOI GRGCYC 3 cut(s) 330, 433, 2079
Fsp4HI GCNGC 8 cut(s) 182, 1374, 1377, 1654, 1819, 2490, 2829, 3029
FspBI CTAG 5 cut(s) 1557, 1716, 1937, 1989, 3059
GlaI GCGC 2 cut(s) 2956, 3011
GluI GCNGC 8 cut(s) 182, 1374, 1377, 1654, 1819, 2490, 2829, 3029
GsuI CTGGAG 2 cut(s) 840, 2367
HaeIII GGCC 8 cut(s) 70, 1028, 1061, 1339, 1974, 2670, 2775, 2886
HapII CCGG 6 cut(s) 434, 632, 872, 1336, 1739, 2187
HhaI GCGC 2 cut(s) 2957, 3012
Hin6I GCGC 2 cut(s) 2955, 3010
HinP1I GCGC 2 cut(s) 2955, 3010
HindIII AAGCTT 3 cut(s) 452, 1031, 2674
HpaII CCGG 6 cut(s) 434, 632, 872, 1336, 1739, 2187
HphI GGTGA 3 cut(s) 283, 395, 1018
Hpy166II GTNNAC 2 cut(s) 514, 1503
Hpy8I GTNNAC 2 cut(s) 514, 1503
Hpy99I CGWCG 2 cut(s) 173, 2053
HpyAV CCTTC 5 cut(s) 69, 1400, 1505, 1658, 2871
HpyCH4IV ACGT 3 cut(s) 920, 2516, 2797
HpyF10VI GCNNNNNNNGC 9 cut(s) 190, 563, 1222, 1904, 2711, 2837, 2857, 2933, 2954
HpyF3I CTNAG 9 cut(s) 72, 281, 302, 648, 1182, 2322, 2601, 2677, 2832
HpySE526I ACGT 3 cut(s) 920, 2516, 2797
HspAI GCGC 2 cut(s) 2955, 3010
Kpn2I TCCGGA 1 cut(s) 433
Ksp22I TGATCA 3 cut(s) 1000, 1482, 1900
Kzo9I GATC 9 cut(s) 18, 121, 1000, 1482, 1546, 1900, 2065, 2628, 2734
LguI GCTCTTC 2 cut(s) 223, 345
LmnI GCTCC 4 cut(s) 190, 436, 2012, 2696
Lsp1109I GCAGC 5 cut(s) 1388, 1640, 1805, 2501, 2815
LweI GCATC 5 cut(s) 1113, 1388, 1649, 2092, 2530
MaeI CTAG 5 cut(s) 1557, 1716, 1937, 1989, 3059
MaeII ACGT 3 cut(s) 920, 2516, 2797
MaeIII GTNAC 8 cut(s) 142, 383, 1812, 2107, 2205, 2354, 2729, 3004
MalI GATC 9 cut(s) 20, 123, 1002, 1484, 1548, 1902, 2067, 2630, 2736
MbiI CCGCTC 1 cut(s) 3028
MboI GATC 9 cut(s) 18, 121, 1000, 1482, 1546, 1900, 2065, 2628, 2734
MfeI CAATTG 2 cut(s) 966, 1952
MflI RGATCY 1 cut(s) 1546
MhlI GDGCHC 5 cut(s) 330, 357, 433, 1736, 2079
MlsI TGGCCA 1 cut(s) 1028
MluNI TGGCCA 1 cut(s) 1028
MlyI GAGTC 6 cut(s) 374, 489, 1310, 2188, 2408, 2978
MmeI TCCRAC 9 cut(s) 114, 168, 531, 1209, 1288, 1299, 1574, 2031, 2170
Mox20I TGGCCA 1 cut(s) 1028
Mph1103I ATGCAT 3 cut(s) 537, 2242, 2944
MroI TCCGGA 1 cut(s) 433
MscI TGGCCA 1 cut(s) 1028
MseI TTAA 6 cut(s) 606, 825, 1454, 1728, 1743, 1875
MslI CAYNNNNRTG 3 cut(s) 1889, 2061, 2927
Msp20I TGGCCA 1 cut(s) 1028
MspA1I CMGCKG 1 cut(s) 1225
MspI CCGG 6 cut(s) 434, 632, 872, 1336, 1739, 2187
MspR9I CCNGG 1 cut(s) 114
MunI CAATTG 2 cut(s) 966, 1952
Mva1269I GAATGC 1 cut(s) 700
MvaI CCWGG 1 cut(s) 114
MvnI CGCG 1 cut(s) 3010
MwoI GCNNNNNNNGC 9 cut(s) 190, 563, 1222, 1904, 2711, 2837, 2857, 2933, 2954
NdeI CATATG 1 cut(s) 2757
NdeII GATC 9 cut(s) 18, 121, 1000, 1482, 1546, 1900, 2065, 2628, 2734
NlaIV GGNNCC 7 cut(s) 432, 939, 1548, 1782, 1973, 2008, 2185
NmeAIII GCCGAG 2 cut(s) 520, 2018
NmuCI GTSAC 3 cut(s) 142, 383, 2354
NsiI ATGCAT 3 cut(s) 537, 2242, 2944
NspI RCATGY 6 cut(s) 133, 1361, 1415, 1888, 2240, 2362
NspV TTCGAA 1 cut(s) 2805
PagI TCATGA 2 cut(s) 1141, 1507
PaqCI CACCTGC 1 cut(s) 1570
PciI ACATGT 3 cut(s) 129, 1357, 1411
PciSI GCTCTTC 2 cut(s) 223, 345
PcsI WCGNNNNNNNCGW 1 cut(s) 2993
PctI GAATGC 1 cut(s) 700
PflMI CCANNNNNTGG 1 cut(s) 1228
PinAI ACCGGT 1 cut(s) 1738
PkrI GCNGC 8 cut(s) 183, 1375, 1378, 1655, 1820, 2491, 2830, 3030
PleI GAGTC 6 cut(s) 373, 488, 1309, 2188, 2408, 2978
PpsI GAGTC 6 cut(s) 373, 488, 1309, 2188, 2408, 2978
PscI ACATGT 3 cut(s) 129, 1357, 1411
Psp124BI GAGCTC 2 cut(s) 330, 2079
Psp6I CCWGG 1 cut(s) 112
PspGI CCWGG 1 cut(s) 112
PspN4I GGNNCC 7 cut(s) 432, 939, 1548, 1782, 1973, 2008, 2185
PspPI GGNCC 2 cut(s) 1258, 1972
PsrI GAACNNNNNNTAC 2 cut(s) 3027, 3059
PstI CTGCAG 2 cut(s) 1581, 2308
PstNI CAGNNNCTG 1 cut(s) 1126
PsuI RGATCY 1 cut(s) 1546
PvuII CAGCTG 1 cut(s) 1225
RsaI GTAC 2 cut(s) 515, 1362
RsaNI GTAC 2 cut(s) 514, 1361
RseI CAYNNNNRTG 3 cut(s) 1889, 2061, 2927
SacI GAGCTC 2 cut(s) 330, 2079
SapI GCTCTTC 2 cut(s) 223, 345
SaqAI TTAA 6 cut(s) 606, 825, 1454, 1728, 1743, 1875
SatI GCNGC 8 cut(s) 182, 1374, 1377, 1654, 1819, 2490, 2829, 3029
Sau3AI GATC 9 cut(s) 18, 121, 1000, 1482, 1546, 1900, 2065, 2628, 2734
Sau96I GGNCC 2 cut(s) 1258, 1972
SchI GAGTC 6 cut(s) 374, 489, 1310, 2188, 2408, 2978
ScrFI CCNGG 1 cut(s) 114
SduI GDGCHC 5 cut(s) 330, 357, 433, 1736, 2079
SfaNI GCATC 5 cut(s) 1113, 1388, 1649, 2092, 2530
SfcI CTRYAG 4 cut(s) 1364, 1577, 2304, 2725
SfuI TTCGAA 1 cut(s) 2805
SinI GGWCC 1 cut(s) 1258
SmiMI CAYNNNNRTG 3 cut(s) 1889, 2061, 2927
SmlI CTYRAG 3 cut(s) 329, 834, 2508
SmoI CTYRAG 3 cut(s) 329, 834, 2508
SsiI CCGC 8 cut(s) 181, 358, 381, 733, 1373, 1544, 1920, 3028
SspI AATATT 2 cut(s) 28, 2635
SspMI CTAG 5 cut(s) 1557, 1716, 1937, 1989, 3059
SstI GAGCTC 2 cut(s) 330, 2079
StyD4I CCNGG 1 cut(s) 112
StyI CCWWGG 3 cut(s) 1062, 1293, 1401
TaiI ACGT 3 cut(s) 923, 2519, 2800
TatI WGTACW 2 cut(s) 513, 1360
TauI GCSGC 3 cut(s) 184, 1376, 3031
Tru1I TTAA 6 cut(s) 606, 825, 1454, 1728, 1743, 1875
Tru9I TTAA 6 cut(s) 606, 825, 1454, 1728, 1743, 1875
TscAI CASTG 5 cut(s) 251, 511, 817, 1617, 1987
TseFI GTSAC 3 cut(s) 142, 383, 2354
TseI GCWGC 5 cut(s) 1376, 1653, 1818, 2489, 2828
Tsp45I GTSAC 3 cut(s) 142, 383, 2354
TspGWI ACGGA 4 cut(s) 1112, 2096, 2709, 2904
TspRI CASTG 5 cut(s) 251, 511, 817, 1617, 1987
Van91I CCANNNNNTGG 1 cut(s) 1228
VpaK11BI GGWCC 1 cut(s) 1258
XagI CCTNNNNNAGG 1 cut(s) 1292
XapI RAATTY 4 cut(s) 1392, 1467, 2365, 2434
XbaI TCTAGA 1 cut(s) 1556
XceI RCATGY 6 cut(s) 133, 1361, 1415, 1888, 2240, 2362
XspI CTAG 5 cut(s) 1557, 1716, 1937, 1989, 3059
Zsp2I ATGCAT 3 cut(s) 537, 2242, 2944
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.