Rmu_sc0005532.1_g000005

Histidine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005532.1
OG0009350
Physical Location & Seq
Forward (+)
15608 .. 20265
4658 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005532.1_g000005.1.cds

Sequence Viewer

Length: 3213 bp
atggtttgtgaaatggagatcgataatattgaagaaatggaaatggaggaagtcctcccttctatgtggcctgaggatatagattccgataagcaattcaacattgaaaagccaggaaaagatcaagacatgttggaggaggtcacattcaaagagaaaccaactacagtcgatttcaggcggctgttggagctaaccaaacacacagatacaggctcttcccaattagcacacctcgtaaagcactgggaatataagcaggcgaatgtggtgagacttcttagagaagaacttgatatcctcagcaagcaaaagcaggaagttgagctcaagaaattggagatattggaagagcaccgcttcgagtcagagagctatggcggtgacaaacgccccatttccattttggatgaagtgtatcagttatgggctccggatgttcctctaaagaaacttgatgttgttgtggagagtaagagagtcgagacagaagccgagtatgacagtgctgtgtactggaagcaaagggctatgcatttggagaagttgttggaggcaagtgttgcgagagagcagattcttgtcgataaattgcaggaaagcattaagagtattgaaagacagtcatcgccggttgatgagctgtctgagattttgaaaagagcagataactttttgcattttgttcttcagaatgcccctgttgttattggtcatcaggataaagagttgcggtatcgctttatctacaatcacttcccaagtttgggagagcaggacattatagggaaaacagatgtcgagattttcagtggggctggagttaaggaatctcaagacttcaagagagaggttctggaaaaagggataccggcgaaaagggagattacattcgagactgaactgtttgggtcaaagacgtttttgatatatgtggaacctgttttcagcaaggcaggggagacaattggtataaactatatggggatggaggtgtctgatcaggtgaggaaaagagagaagatggcaaagcttcgtgaggaaatagccgtccaaaaggccaaggaaacagagctgaacaagacaattcacataacggaggagacaatgagagcaaaacagatgctggcaacaatgtctcatgagataaggtctcctctttctggggttgtcagcatggctgagattcttactaccacgaaacttgaccgcgagcaacgccagctgttggaagtcatgttatcttcaggagatttggtcctccaactgataaatgacatccttgacctttccaaggttgagtcaggagtaatgaagttggaggctacaaagttccggccaagacaggtagttagacatgtactacagactgcggcagcatcattgcagaaaattttgaccttggaaggacatgttatggagaatgttcctgtggaggtcattggagatgttttaaggattcgtcaaattctaaccaacttgatcagtaatgcaatcaagtttactcatgaagggaaagtcgggataaaactttacatggtagcggatccaacttctggagatgatgaaagatgtctgcagatgtcgaatgcagttcaatcaacggtttcagtgaatggattggatgaagagaaatcagcatcacaaaattgctgcaatggagaaggtattcgtcgcaaagaaccttaccaaaatggttctcttcacgatgaacctagcacaccggttaagagtgaagcctcaatgaatgcagataaagaggacgaaccccattcatctgaaacaacaacagtgtggttacgctgcgatgtatatgacacaggaattggaataccagaaaatgctttaccgaccctttttaaaagatacatgcaagtgagtgctgatcatgctcgaaaatatggcgggactggattaggactagcaatatgcaaacaattggttgaactaatggggggccaactcactgtgtttagccgagaacattgtggctccacattcacatttgtattaccttacaaggtttcaacatcgtcggaacattctgatgatcctgatgagctcacggatatcgctgaatatgacgatgctgtaactgatgatgaagtagctgaaagctatttccagttccaaccacgcactttaggttctctgttctcttctaatggcaccggcaggactcaaaaactgttaccacataaactcaatggattatcacaacatgcatactcgtttccatataataacatcagaccagaggagattacttcagttgaaaattgctcttctgcagtcgatgatgttgctgagacatctgaaccagagagttcagtaagtcacatgccgaattccaatagcgaaactccagcatgtagggacagaaagggtcaagatgactcaaacagtcaatcccaaaattcttcaacagattcaaatcatcatgcagaggcaagcagagaaaatggtgtagcagcaaagacaagagaaccccaaggaacgtgtaatagacaggagaaatcagatgcagattcagagtgtagctctggcaacagtccaaaaataccaaaatcaacatcgaatcctaagattcttcttgttgaagataacaagatcaatattatggtggcacagtcaatgatgaagaagttaggccaaagcttagaggttgtaaatgacggagctgaagctgtgcgtgcagttcaacgctgtagttacgatctgattctgatggatgttcatatgccagttatggatggcctacaagctacaagaataatacgttctttcgaagaaactggcagctgggaagctgctaagaaagctggaattgagcaatctgtgccttgttcaaatacaccacaaaacagccacggacatacgccttgtagaaagcgaatcccaatcattgcgatgacagcaaatgcatttgcagagagcgcagaagagtgttttgcaaatggtatggactcgtttgtgccgaaacctgaaggcgaggttacggacgtagatgactcagttgacctccggcggcagccgcgtcttgactttccggagactgattgcgctcatcttgttggggttttggggtcaagattgctcggaatgttggcgcttcgagaagtaagagaggatagaagaaccaggatagaaaagatgtgggccttttattacaatttgggcccggccccatacattacagtgggtctagactag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000302 GO:0002237 GO:0003674 GO:0003824 GO:0004672 GO:0004673 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009617 GO:0009636 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009755 GO:0009787 GO:0009788 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010104 GO:0010105 GO:0010119 GO:0010646 GO:0010648 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016775 GO:0018106 GO:0018193 GO:0018202 GO:0019538 GO:0022622 GO:0023051 GO:0023052 GO:0023057 GO:0032501 GO:0032502 GO:0032870 GO:0033554 GO:0034599 GO:0034614 GO:0035690 GO:0036211 GO:0042221 GO:0042493 GO:0042542 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046677 GO:0048364 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0070297 GO:0070298 GO:0070301 GO:0070887 GO:0071216 GO:0071219 GO:0071236 GO:0071241 GO:0071310 GO:0071368 GO:0071495 GO:0071704 GO:0071731 GO:0071732 GO:0071944 GO:0090333 GO:0097237 GO:0097366 GO:0099402 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902170 GO:1902531 GO:1902532 GO:1905957 GO:1905958
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HATPase_c HisKA Response_reg

Protein Analysis

1070

Amino Acids

120.21

Weight (kDa)

5.06

Isoelectric Point (pI)

52.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 2168
AccB7I CCANNNNNTGG 2 cut(s) 1228, 1556
AccII CGCG 2 cut(s) 1212, 3037
AccIII TCCGGA 2 cut(s) 433, 3049
AclWI GGATC 3 cut(s) 1541, 1554, 2045
AcoI YGGCCR 1 cut(s) 1337
AcsI RAATTY 4 cut(s) 1392, 1467, 2347, 2416
AcuI CTGAAG 5 cut(s) 674, 1230, 2253, 2703, 3006
AfaI GTAC 2 cut(s) 515, 1362
AfiI CCNNNNNNNGG 6 cut(s) 766, 767, 1163, 1228, 1294, 1556
AflIII ACRYGT 4 cut(s) 129, 1357, 1411, 2498
AgeI ACCGGT 1 cut(s) 1723
AjnI CCWGG 2 cut(s) 112, 3140
AloI GAACNNNNNNTCC 2 cut(s) 282, 314
Alw21I GWGCWC 3 cut(s) 330, 357, 2064
Alw26I GTCTC 9 cut(s) 268, 479, 890, 956, 1097, 1143, 1158, 2303, 3047
AlwI GGATC 3 cut(s) 1541, 1554, 2045
AlwNI CAGNNNCTG 1 cut(s) 1126
Aor13HI TCCGGA 2 cut(s) 433, 3049
AoxI GGCC 9 cut(s) 68, 1059, 1337, 1957, 2650, 2755, 3159, 3178, 3183
ApaI GGGCCC 1 cut(s) 3182
ApeKI GCWGC 7 cut(s) 1376, 1653, 1803, 2471, 2799, 2810, 3031
ApoI RAATTY 4 cut(s) 1392, 1467, 2347, 2416
AsiGI ACCGGT 1 cut(s) 1723
Asp700I GAANNNNTTC 1 cut(s) 1668
AspLEI GCGC 3 cut(s) 2939, 3065, 3112
AspS9I GGNCC 6 cut(s) 1258, 1957, 3159, 3178, 3179, 3184
AsuC2I CCSGG 1 cut(s) 3182
AsuHPI GGTGA 3 cut(s) 283, 395, 1018
AsuII TTCGAA 1 cut(s) 2787
AvaII GGWCC 1 cut(s) 1258
AxyI CCTNAGG 1 cut(s) 72
BaeGI GKGCMC 1 cut(s) 3182
BamHI GGATCC 1 cut(s) 1546
BanI GGYRCC 1 cut(s) 2168
BanII GRGCYC 4 cut(s) 330, 433, 2064, 3182
Bbv12I GWGCWC 3 cut(s) 330, 357, 2064
BbvCI CCTCAGC 1 cut(s) 302
BbvI GCAGC 7 cut(s) 1388, 1640, 1790, 2483, 2797, 2811, 3043
BccI CCATC 4 cut(s) 982, 1018, 2722, 2747
BceAI ACGGC 1 cut(s) 1034
BcgI CGANNNNNNTGC 2 cut(s) 2285, 2319
BciT130I CCWGG 2 cut(s) 114, 3142
BciVI GTATCC 1 cut(s) 861
BclI TGATCA 3 cut(s) 1000, 1482, 1885
BcnI CCSGG 1 cut(s) 3182
BcoDI GTCTC 9 cut(s) 268, 479, 890, 956, 1097, 1143, 1158, 2303, 3047
BfaI CTAG 4 cut(s) 1716, 1922, 3206, 3211
BfmI CTRYAG 5 cut(s) 165, 1364, 1577, 2289, 2707
BfoI RGCGCY 1 cut(s) 3113
BfuI GTATCC 1 cut(s) 861
Bme1390I CCNGG 3 cut(s) 114, 3142, 3182
Bme18I GGWCC 1 cut(s) 1258
BmgT120I GGNCC 6 cut(s) 1258, 1957, 3159, 3178, 3179, 3184
BmiI GGNNCC 8 cut(s) 432, 939, 1548, 1958, 1993, 2170, 3180, 3186
BmrFI CCNGG 3 cut(s) 114, 3142, 3182
BmrI ACTGGG 1 cut(s) 256
BmsI GCATC 5 cut(s) 1113, 1388, 1649, 2077, 2512
BmuI ACTGGG 1 cut(s) 256
BplI GAGNNNNNCTC 2 cut(s) 2525, 2557
BpmI CTGGAG 3 cut(s) 840, 1578, 2349
Bpu10I CCTNAGC 1 cut(s) 302
Bpu14I TTCGAA 1 cut(s) 2787
BpuEI CTTGAG 2 cut(s) 314, 819
BpuMI CCSGG 1 cut(s) 3182
Bsa29I ATCGAT 1 cut(s) 21
BsaBI GATNNNNATC 1 cut(s) 2433
BsaI GGTCTC 1 cut(s) 1158
BsaJI CCNNGG 5 cut(s) 1062, 1293, 1401, 2491, 2869
BsaWI WCCGGW 3 cut(s) 433, 1723, 3049
BsaXI ACNNNNNCTCC 4 cut(s) 533, 563, 1242, 1272
Bsc4I CCNNNNNNNGG 6 cut(s) 766, 767, 1163, 1228, 1294, 1556
Bse118I RCCGGY 4 cut(s) 631, 871, 1723, 2171
Bse1I ACTGG 6 cut(s) 251, 521, 1915, 2125, 2744, 2800
Bse21I CCTNAGG 1 cut(s) 72
Bse3DI GCAATG 3 cut(s) 1382, 1663, 2904
Bse8I GATNNNNATC 1 cut(s) 2433
BseAI TCCGGA 2 cut(s) 433, 3049
BseBI CCWGG 2 cut(s) 114, 3142
BseCI ATCGAT 1 cut(s) 21
BseDI CCNNGG 5 cut(s) 1062, 1293, 1401, 2491, 2869
BseGI GGATG 7 cut(s) 415, 442, 993, 1278, 1630, 2737, 2758
BseJI GATNNNNATC 1 cut(s) 2433
BseLI CCNNNNNNNGG 6 cut(s) 766, 767, 1163, 1228, 1294, 1556
BseMI GCAATG 3 cut(s) 1382, 1663, 2904
BseMII CTCAG 6 cut(s) 63, 316, 639, 1173, 2298, 3027
BseNI ACTGG 6 cut(s) 251, 521, 1915, 2125, 2744, 2800
BseRI GAGGAG 4 cut(s) 152, 1115, 1146, 2273
BseSI GKGCMC 1 cut(s) 3182
BseXI GCAGC 7 cut(s) 1388, 1640, 1790, 2483, 2797, 2811, 3043
BseYI CCCAGC 1 cut(s) 2802
BsgI GTGCAG 1 cut(s) 2715
Bsh1236I CGCG 2 cut(s) 1212, 3037
BshFI GGCC 9 cut(s) 70, 1061, 1339, 1959, 2652, 2757, 3161, 3180, 3185
BshNI GGYRCC 1 cut(s) 2168
BshTI ACCGGT 1 cut(s) 1723
BshVI ATCGAT 1 cut(s) 21
BsiHKAI GWGCWC 3 cut(s) 330, 357, 2064
BsiSI CCGG 9 cut(s) 434, 632, 872, 1336, 1724, 2172, 3025, 3050, 3182
BslFI GGGAC 2 cut(s) 1921, 2390
BslI CCNNNNNNNGG 6 cut(s) 766, 767, 1163, 1228, 1294, 1556
BsmAI GTCTC 9 cut(s) 268, 479, 890, 956, 1097, 1143, 1158, 2303, 3047
BsmFI GGGAC 2 cut(s) 1921, 2390
BsmI GAATGC 3 cut(s) 700, 1594, 1753
BsnI GGCC 9 cut(s) 70, 1061, 1339, 1959, 2652, 2757, 3161, 3180, 3185
Bso31I GGTCTC 1 cut(s) 1158
Bsp119I TTCGAA 1 cut(s) 2787
Bsp120I GGGCCC 1 cut(s) 3178
Bsp1286I GDGCHC 5 cut(s) 330, 357, 433, 2064, 3182
Bsp13I TCCGGA 2 cut(s) 433, 3049
Bsp143I GATC 9 cut(s) 18, 121, 1000, 1482, 1546, 1885, 2050, 2610, 2716
BspANI GGCC 9 cut(s) 70, 1061, 1339, 1959, 2652, 2757, 3161, 3180, 3185
BspCNI CTCAG 6 cut(s) 64, 315, 640, 1174, 2299, 3026
BspDI ATCGAT 1 cut(s) 21
BspEI TCCGGA 2 cut(s) 433, 3049
BspFNI CGCG 2 cut(s) 1212, 3037
BspHI TCATGA 2 cut(s) 1141, 1507
BspLI GGNNCC 8 cut(s) 432, 939, 1548, 1958, 1993, 2170, 3180, 3186
BspMAI CTGCAG 2 cut(s) 1581, 2293
BspPI GGATC 3 cut(s) 1541, 1554, 2045
BspQI GCTCTTC 3 cut(s) 223, 345, 2290
BspT104I TTCGAA 1 cut(s) 2787
BspT107I GGYRCC 1 cut(s) 2168
BspTNI GGTCTC 1 cut(s) 1158
BsrDI GCAATG 3 cut(s) 1382, 1663, 2904
BsrFI RCCGGY 4 cut(s) 631, 871, 1723, 2171
BsrI ACTGG 6 cut(s) 251, 521, 1915, 2125, 2744, 2800
BssAI RCCGGY 4 cut(s) 631, 871, 1723, 2171
BssECI CCNNGG 5 cut(s) 1062, 1293, 1401, 2491, 2869
BssMI GATC 9 cut(s) 18, 121, 1000, 1482, 1546, 1885, 2050, 2610, 2716
BssT1I CCWWGG 4 cut(s) 1062, 1293, 1401, 2491
Bst2UI CCWGG 2 cut(s) 114, 3142
Bst6I CTCTTC 7 cut(s) 223, 345, 1623, 1707, 2164, 2290, 2937
BstAPI GCANNNNNTGC 2 cut(s) 563, 2839
BstBI TTCGAA 1 cut(s) 2787
BstC8I GCNNGC 7 cut(s) 261, 308, 1128, 1214, 1223, 2452, 2694
BstDSI CCRYGG 1 cut(s) 2869
BstENI CCTNNNNNAGG 1 cut(s) 1292
BstF5I GGATG 7 cut(s) 415, 442, 993, 1278, 1630, 2737, 2758
BstFNI CGCG 2 cut(s) 1212, 3037
BstH2I RGCGCY 1 cut(s) 3113
BstHHI GCGC 3 cut(s) 2939, 3065, 3112
BstKTI GATC 9 cut(s) 21, 124, 1003, 1485, 1549, 1888, 2053, 2613, 2719
BstMAI GTCTC 9 cut(s) 268, 479, 890, 956, 1097, 1143, 1158, 2303, 3047
BstMBI GATC 9 cut(s) 18, 121, 1000, 1482, 1546, 1885, 2050, 2610, 2716
BstNI CCWGG 2 cut(s) 114, 3142
BstNSI RCATGY 7 cut(s) 133, 1361, 1415, 1873, 2225, 2344, 2373
BstSCI CCNGG 3 cut(s) 112, 3140, 3180
BstSFI CTRYAG 5 cut(s) 165, 1364, 1577, 2289, 2707
BstSLI GKGCMC 1 cut(s) 3182
BstUI CGCG 2 cut(s) 1212, 3037
BstV1I GCAGC 7 cut(s) 1388, 1640, 1790, 2483, 2797, 2811, 3043
BstX2I RGATCY 1 cut(s) 1546
BstYI RGATCY 1 cut(s) 1546
Bsu15I ATCGAT 1 cut(s) 21
Bsu36I CCTNAGG 1 cut(s) 72
BsuI GTATCC 1 cut(s) 861
BsuRI GGCC 9 cut(s) 70, 1061, 1339, 1959, 2652, 2757, 3161, 3180, 3185
BsuTUI ATCGAT 1 cut(s) 21
BtgI CCRYGG 1 cut(s) 2869
BtgZI GCGATG 3 cut(s) 612, 1821, 2924
BtsCI GGATG 7 cut(s) 415, 442, 993, 1278, 1630, 2737, 2758
BtsIMutI CAGTG 7 cut(s) 244, 511, 817, 1617, 1797, 1965, 3204
Cac8I GCNNGC 7 cut(s) 261, 308, 1128, 1214, 1223, 2452, 2694
CaiI CAGNNNCTG 1 cut(s) 1126
CciI TCATGA 2 cut(s) 1141, 1507
CfoI GCGC 3 cut(s) 2939, 3065, 3112
Cfr10I RCCGGY 4 cut(s) 631, 871, 1723, 2171
Cfr13I GGNCC 6 cut(s) 1258, 1957, 3159, 3178, 3179, 3184
ClaI ATCGAT 1 cut(s) 21
CseI GACGC 1 cut(s) 3026
Csp6I GTAC 2 cut(s) 514, 1361
CspAI ACCGGT 1 cut(s) 1723
CspCI CAANNNNNGTGG 2 cut(s) 1186, 1221
CviQI GTAC 2 cut(s) 514, 1361
DpnI GATC 9 cut(s) 20, 123, 1002, 1484, 1548, 1887, 2052, 2612, 2718
DpnII GATC 9 cut(s) 18, 121, 1000, 1482, 1546, 1885, 2050, 2610, 2716
DraI TTTAAA 1 cut(s) 1861
EaeI YGGCCR 1 cut(s) 1337
Eam1104I CTCTTC 7 cut(s) 223, 345, 1623, 1707, 2164, 2290, 2937
EarI CTCTTC 7 cut(s) 223, 345, 1623, 1707, 2164, 2290, 2937
Ecl136II GAGCTC 2 cut(s) 328, 2062
Eco130I CCWWGG 4 cut(s) 1062, 1293, 1401, 2491
Eco24I GRGCYC 4 cut(s) 330, 433, 2064, 3182
Eco31I GGTCTC 1 cut(s) 1158
Eco32I GATATC 2 cut(s) 298, 2071
Eco47I GGWCC 1 cut(s) 1258
Eco53kI GAGCTC 2 cut(s) 328, 2062
Eco57I CTGAAG 5 cut(s) 674, 1230, 2253, 2703, 3006
Eco81I CCTNAGG 1 cut(s) 72
EcoICRI GAGCTC 2 cut(s) 328, 2062
EcoNI CCTNNNNNAGG 1 cut(s) 1292
EcoRI GAATTC 1 cut(s) 2347
EcoRII CCWGG 2 cut(s) 112, 3140
EcoRV GATATC 2 cut(s) 298, 2071
EcoT14I CCWWGG 4 cut(s) 1062, 1293, 1401, 2491
EcoT22I ATGCAT 3 cut(s) 537, 2227, 2926
EcoT38I GRGCYC 4 cut(s) 330, 433, 2064, 3182
ErhI CCWWGG 4 cut(s) 1062, 1293, 1401, 2491
FaqI GGGAC 2 cut(s) 1921, 2390
FauI CCCGC 1 cut(s) 1898
FauNDI CATATG 1 cut(s) 2739
FbaI TGATCA 3 cut(s) 1000, 1482, 1885
FokI GGATG 7 cut(s) 422, 449, 1000, 1265, 1637, 2744, 2765
FriOI GRGCYC 4 cut(s) 330, 433, 2064, 3182
FspBI CTAG 4 cut(s) 1716, 1922, 3206, 3211
GlaI GCGC 3 cut(s) 2938, 3064, 3111
GsaI CCCAGC 1 cut(s) 2806
GsuI CTGGAG 3 cut(s) 840, 1578, 2349
HaeII RGCGCY 1 cut(s) 3113
HaeIII GGCC 9 cut(s) 70, 1061, 1339, 1959, 2652, 2757, 3161, 3180, 3185
HapII CCGG 9 cut(s) 434, 632, 872, 1336, 1724, 2172, 3025, 3050, 3182
HgaI GACGC 1 cut(s) 3026
HhaI GCGC 3 cut(s) 2939, 3065, 3112
Hin6I GCGC 3 cut(s) 2937, 3063, 3110
HinP1I GCGC 3 cut(s) 2937, 3063, 3110
HincII GTYRAC 1 cut(s) 3019
HindII GTYRAC 1 cut(s) 3019
HindIII AAGCTT 2 cut(s) 1031, 2656
HpaII CCGG 9 cut(s) 434, 632, 872, 1336, 1724, 2172, 3025, 3050, 3182
HphI GGTGA 3 cut(s) 283, 395, 1018
Hpy166II GTNNAC 3 cut(s) 514, 1503, 3019
Hpy8I GTNNAC 3 cut(s) 514, 1503, 3019
Hpy99I CGWCG 2 cut(s) 1677, 2038
HpyAV CCTTC 5 cut(s) 69, 1400, 1505, 1658, 2981
HpyCH4IV ACGT 4 cut(s) 920, 2498, 2779, 3003
HpySE526I ACGT 4 cut(s) 920, 2498, 2779, 3003
HspAI GCGC 3 cut(s) 2937, 3063, 3110
Kpn2I TCCGGA 2 cut(s) 433, 3049
Ksp22I TGATCA 3 cut(s) 1000, 1482, 1885
Kzo9I GATC 9 cut(s) 18, 121, 1000, 1482, 1546, 1885, 2050, 2610, 2716
LguI GCTCTTC 3 cut(s) 223, 345, 2290
LmnI GCTCC 4 cut(s) 190, 436, 1997, 2678
Lsp1109I GCAGC 7 cut(s) 1388, 1640, 1790, 2483, 2797, 2811, 3043
LweI GCATC 5 cut(s) 1113, 1388, 1649, 2077, 2512
MaeI CTAG 4 cut(s) 1716, 1922, 3206, 3211
MaeII ACGT 4 cut(s) 920, 2498, 2779, 3003
MaeIII GTNAC 8 cut(s) 142, 383, 1797, 2092, 2190, 2336, 2711, 2995
MalI GATC 9 cut(s) 20, 123, 1002, 1484, 1548, 1887, 2052, 2612, 2718
MboI GATC 9 cut(s) 18, 121, 1000, 1482, 1546, 1885, 2050, 2610, 2716
MfeI CAATTG 2 cut(s) 966, 1937
MflI RGATCY 1 cut(s) 1546
MhlI GDGCHC 5 cut(s) 330, 357, 433, 2064, 3182
MlyI GAGTC 7 cut(s) 374, 489, 1310, 2173, 2390, 2960, 3005
MmeI TCCRAC 9 cut(s) 114, 168, 531, 1209, 1288, 1299, 1574, 2016, 2155
Mph1103I ATGCAT 3 cut(s) 537, 2227, 2926
MroI TCCGGA 2 cut(s) 433, 3049
MroXI GAANNNNTTC 1 cut(s) 1668
MseI TTAA 5 cut(s) 606, 825, 1454, 1728, 1860
MslI CAYNNNNRTG 4 cut(s) 1874, 2046, 2909, 3197
MspA1I CMGCKG 2 cut(s) 1225, 2802
MspI CCGG 9 cut(s) 434, 632, 872, 1336, 1724, 2172, 3025, 3050, 3182
MspR9I CCNGG 3 cut(s) 114, 3142, 3182
MunI CAATTG 2 cut(s) 966, 1937
Mva1269I GAATGC 3 cut(s) 700, 1594, 1753
MvaI CCWGG 2 cut(s) 114, 3142
MvnI CGCG 2 cut(s) 1212, 3037
NciI CCSGG 1 cut(s) 3182
NdeI CATATG 1 cut(s) 2739
NdeII GATC 9 cut(s) 18, 121, 1000, 1482, 1546, 1885, 2050, 2610, 2716
NlaIV GGNNCC 8 cut(s) 432, 939, 1548, 1958, 1993, 2170, 3180, 3186
NmeAIII GCCGAG 2 cut(s) 520, 2003
NmuCI GTSAC 3 cut(s) 142, 383, 2336
NsiI ATGCAT 3 cut(s) 537, 2227, 2926
NspI RCATGY 7 cut(s) 133, 1361, 1415, 1873, 2225, 2344, 2373
NspV TTCGAA 1 cut(s) 2787
PagI TCATGA 2 cut(s) 1141, 1507
PciI ACATGT 3 cut(s) 129, 1357, 1411
PciSI GCTCTTC 3 cut(s) 223, 345, 2290
PcsI WCGNNNNNNNCGW 1 cut(s) 2975
PctI GAATGC 3 cut(s) 700, 1594, 1753
PdmI GAANNNNTTC 1 cut(s) 1668
PflMI CCANNNNNTGG 2 cut(s) 1228, 1556
PinAI ACCGGT 1 cut(s) 1723
PleI GAGTC 7 cut(s) 373, 488, 1309, 2173, 2390, 2960, 3005
PpsI GAGTC 7 cut(s) 373, 488, 1309, 2173, 2390, 2960, 3005
PscI ACATGT 3 cut(s) 129, 1357, 1411
Psp124BI GAGCTC 2 cut(s) 330, 2064
Psp6I CCWGG 2 cut(s) 112, 3140
PspFI CCCAGC 1 cut(s) 2802
PspGI CCWGG 2 cut(s) 112, 3140
PspN4I GGNNCC 8 cut(s) 432, 939, 1548, 1958, 1993, 2170, 3180, 3186
PspOMI GGGCCC 1 cut(s) 3178
PspPI GGNCC 6 cut(s) 1258, 1957, 3159, 3178, 3179, 3184
PstI CTGCAG 2 cut(s) 1581, 2293
PstNI CAGNNNCTG 1 cut(s) 1126
PsuI RGATCY 1 cut(s) 1546
PvuII CAGCTG 2 cut(s) 1225, 2802
RsaI GTAC 2 cut(s) 515, 1362
RsaNI GTAC 2 cut(s) 514, 1361
RseI CAYNNNNRTG 4 cut(s) 1874, 2046, 2909, 3197
SacI GAGCTC 2 cut(s) 330, 2064
SapI GCTCTTC 3 cut(s) 223, 345, 2290
SaqAI TTAA 5 cut(s) 606, 825, 1454, 1728, 1860
Sau3AI GATC 9 cut(s) 18, 121, 1000, 1482, 1546, 1885, 2050, 2610, 2716
Sau96I GGNCC 6 cut(s) 1258, 1957, 3159, 3178, 3179, 3184
SchI GAGTC 7 cut(s) 374, 489, 1310, 2173, 2390, 2960, 3005
ScrFI CCNGG 3 cut(s) 114, 3142, 3182
SduI GDGCHC 5 cut(s) 330, 357, 433, 2064, 3182
SfaNI GCATC 5 cut(s) 1113, 1388, 1649, 2077, 2512
SfcI CTRYAG 5 cut(s) 165, 1364, 1577, 2289, 2707
SfuI TTCGAA 1 cut(s) 2787
SinI GGWCC 1 cut(s) 1258
SmiMI CAYNNNNRTG 4 cut(s) 1874, 2046, 2909, 3197
SmlI CTYRAG 2 cut(s) 329, 834
SmoI CTYRAG 2 cut(s) 329, 834
SspI AATATT 2 cut(s) 28, 2617
SspMI CTAG 4 cut(s) 1716, 1922, 3206, 3211
SstI GAGCTC 2 cut(s) 330, 2064
StyD4I CCNGG 3 cut(s) 112, 3140, 3180
StyI CCWWGG 4 cut(s) 1062, 1293, 1401, 2491
TaiI ACGT 4 cut(s) 923, 2501, 2782, 3006
TatI WGTACW 2 cut(s) 513, 1360
TauI GCSGC 4 cut(s) 184, 1376, 3031, 3037
Tru1I TTAA 5 cut(s) 606, 825, 1454, 1728, 1860
Tru9I TTAA 5 cut(s) 606, 825, 1454, 1728, 1860
TscAI CASTG 7 cut(s) 251, 511, 817, 1617, 1797, 1972, 3204
TseFI GTSAC 3 cut(s) 142, 383, 2336
TseI GCWGC 7 cut(s) 1376, 1653, 1803, 2471, 2799, 2810, 3031
Tsp45I GTSAC 3 cut(s) 142, 383, 2336
TspGWI ACGGA 5 cut(s) 1112, 2081, 2691, 2886, 3014
TspRI CASTG 7 cut(s) 251, 511, 817, 1617, 1797, 1972, 3204
Van91I CCANNNNNTGG 2 cut(s) 1228, 1556
VpaK11BI GGWCC 1 cut(s) 1258
XagI CCTNNNNNAGG 1 cut(s) 1292
XapI RAATTY 4 cut(s) 1392, 1467, 2347, 2416
XbaI TCTAGA 1 cut(s) 3205
XceI RCATGY 7 cut(s) 133, 1361, 1415, 1873, 2225, 2344, 2373
XcmI CCANNNNNNNNNTGG 1 cut(s) 403
XmnI GAANNNNTTC 1 cut(s) 1668
XspI CTAG 4 cut(s) 1716, 1922, 3206, 3211
Zsp2I ATGCAT 3 cut(s) 537, 2227, 2926
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.