pycom08g03990

Histidine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0009350
Physical Location & Seq
Forward (+)
3010075 .. 3011773
1699 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g03990.4

Sequence Viewer

Length: 1332 bp
ATGGATGCAGCAGATCAAGAGGACGAACCTCATTTGCCAGAAACGAAAACGGTTTGGTTACGATGCGATGTATACGACACCGGAATCGGAATACCAGAAAATGCTTTACCTTCTCTGTTTAAAAGATACATGCAAGTCAGTGCCGATCATGCTCGGAAATATGGAGGGACAGGATTAGGTCTAGCAATATGCAAACAATTGGTCGAGCTAATGGGGGGTCAACTCACAGTATCTAGCAAAGAAAAACGTGGGTCGACATTTACATTTGTACTCCCGTACAAGGTCTCGACATCGTCGGAACAACAATCCGATGACCCCGATGATTCTGACGAAGCTTCGGATATGGCTAGTCATGATGGCGCAACTGATGAAGCAGCTGAGAGCTATTTCCAGTTCCAGCCGCGGACTTTGGGTTCTCTGTTCTCCTCTAATGGATCCGCCAGGACACCAAAATTGTTACCGCAAAAGATGGGGTTGACTGGTTCGCATAAACTCAATGGGCTTGAACAAAATTCATACTCGTTTCCGAACGGTAACATGAGACCGGAGGAGATAGCTTCGGTTGAAAACTCTTGCTCGGAAGGCGATTTTGCGGAGACGTTATCAGAACCAGAGAGTTCCTCAGCGAGTCGCAACCGAGACCCTAGTAGTGAAAATCCAGCTAGTACAAACAAACAGGGTCGAGATGATATGGATAACCGATTCCAAGACTCTATAAGTACAGATTCAAGTCATCCTTCAGCGGAAGGAAGAGAAATAGCTGTAGAAGCACCCACAAGAGAATCCCAAGGTACATGTAAGAGGCAGGAGAAACCCGACGCAGACTCGGACAGTACTTGTAGCAGCAGTTCAAAAATACCAAAACCAACATCAAAACCTAAAATTCTTCTTGTTGAAGATAACAAGATTAACGTTATGGTGGCACAGAACATGATGAAGCGGCTAGGCCATAGCATGGATGTTGTGAACAACGGAATGGAAGCTGTGCGTGCAGTTCAACGCTGTAGATACGATCTCATCCTCATGGATGTTCACATGCCAGTTATGGATGGCCTTCAAACTACAAGACTTATACGTTCTTATGAAGAAACTGGCAACTGGGATGCAGCTGAGAAAGCTGGAATTGAGCTTCCTCTGCCTTCATCAGAAACAACACAGAACGGTCATGGCTACACGCCTTGTGGAAAGCGAATCCCGATCATAGCGATGACAGCAAATGCATTTTCAGAGAGTGCAGAAGAATGTTTCAAAAGTGGAATGGACTCATTCGTGCCGAAGCCTGTAACGTTTCAAAAATTGAAAGACCGTCTCGAACAATACCTACCCGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000302 GO:0002237 GO:0003674 GO:0003824 GO:0004672 GO:0004673 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009617 GO:0009636 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009755 GO:0009787 GO:0009788 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010104 GO:0010105 GO:0010119 GO:0010646 GO:0010648 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016775 GO:0018106 GO:0018193 GO:0018202 GO:0019538 GO:0022622 GO:0023051 GO:0023052 GO:0023057 GO:0032501 GO:0032502 GO:0032870 GO:0033554 GO:0034599 GO:0034614 GO:0035690 GO:0036211 GO:0042221 GO:0042493 GO:0042542 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046677 GO:0048364 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0070297 GO:0070298 GO:0070301 GO:0070887 GO:0071216 GO:0071219 GO:0071236 GO:0071241 GO:0071310 GO:0071368 GO:0071495 GO:0071704 GO:0071731 GO:0071732 GO:0071944 GO:0090333 GO:0097237 GO:0097366 GO:0099402 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902170 GO:1902531 GO:1902532 GO:1905957 GO:1905958
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HATPase_c HisKA Response_reg

Protein Analysis

444

Amino Acids

48.61

Weight (kDa)

4.9

Isoelectric Point (pI)

47.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 955
AccI GTMKAC 2 cut(s) 72, 254
AccII CGCG 1 cut(s) 403
AciI CCGC 7 cut(s) 401, 403, 438, 461, 593, 743, 940
AclI AACGTT 2 cut(s) 912, 1286
AclWI GGATC 2 cut(s) 429, 442
AcsI RAATTY 2 cut(s) 511, 882
AcuI CTGAAG 1 cut(s) 723
AfaI GTAC 6 cut(s) 270, 278, 667, 721, 793, 835
AfiI CCNNNNNNNGG 2 cut(s) 280, 955
AflIII ACRYGT 1 cut(s) 794
AjnI CCWGG 1 cut(s) 440
AloI GAACNNNNNNTCC 2 cut(s) 397, 429
Alw26I GTCTC 5 cut(s) 289, 535, 590, 633, 1313
AlwI GGATC 2 cut(s) 429, 442
AoxI GGCC 2 cut(s) 946, 1051
ApeKI GCWGC 4 cut(s) 8, 374, 843, 1106
ApoI RAATTY 2 cut(s) 511, 882
ArsI GACNNNNNNTTYG 2 cut(s) 186, 218
AspLEI GCGC 1 cut(s) 362
BamHI GGATCC 1 cut(s) 434
BbvCI CCTCAGC 1 cut(s) 622
BbvI GCAGC 4 cut(s) 20, 386, 855, 1118
BccI CCATC 3 cut(s) 350, 463, 1043
BciT130I CCWGG 1 cut(s) 442
BcoDI GTCTC 5 cut(s) 289, 535, 590, 633, 1313
BfaI CTAG 6 cut(s) 182, 234, 348, 645, 663, 944
BfmI CTRYAG 2 cut(s) 762, 1003
BisI GCNGC 6 cut(s) 9, 375, 401, 844, 941, 1107
BlsI GCNGC 6 cut(s) 10, 376, 402, 845, 942, 1108
BmcAI AGTACT 1 cut(s) 835
Bme1390I CCNGG 1 cut(s) 442
BmiI GGNNCC 1 cut(s) 436
BmrFI CCNGG 1 cut(s) 442
BmrI ACTGGG 1 cut(s) 1108
BmsI GCATC 2 cut(s) 53, 1093
BmuI ACTGGG 1 cut(s) 1108
BplI GAGNNNNNCTC 2 cut(s) 605, 637
Bpu10I CCTNAGC 1 cut(s) 622
BsaI GGTCTC 3 cut(s) 289, 535, 633
BsaJI CCNNGG 2 cut(s) 401, 787
BsaWI WCCGGW 2 cut(s) 80, 544
Bsc4I CCNNNNNNNGG 2 cut(s) 280, 955
Bse1I ACTGG 5 cut(s) 391, 484, 1040, 1096, 1103
BseBI CCWGG 1 cut(s) 442
BseDI CCNNGG 2 cut(s) 401, 787
BseGI GGATG 7 cut(s) 10, 733, 964, 1017, 1033, 1054, 1108
BseLI CCNNNNNNNGG 2 cut(s) 280, 955
BseMII CTCAG 3 cut(s) 369, 636, 1101
BseNI ACTGG 5 cut(s) 391, 484, 1040, 1096, 1103
BseRI GAGGAG 2 cut(s) 415, 563
BseXI GCAGC 4 cut(s) 20, 386, 855, 1118
BsgI GTGCAG 2 cut(s) 1011, 1254
Bsh1236I CGCG 1 cut(s) 403
BshFI GGCC 2 cut(s) 948, 1053
BsiSI CCGG 2 cut(s) 81, 545
BslFI GGGAC 1 cut(s) 181
BslI CCNNNNNNNGG 2 cut(s) 280, 955
BsmAI GTCTC 5 cut(s) 289, 535, 590, 633, 1313
BsmBI CGTCTC 2 cut(s) 590, 1313
BsmFI GGGAC 1 cut(s) 181
BsnI GGCC 2 cut(s) 948, 1053
Bso31I GGTCTC 3 cut(s) 289, 535, 633
Bsp143I GATC 5 cut(s) 13, 145, 434, 1012, 1197
BspACI CCGC 7 cut(s) 401, 403, 438, 461, 593, 743, 940
BspANI GGCC 2 cut(s) 948, 1053
BspCNI CTCAG 3 cut(s) 370, 635, 1102
BspFNI CGCG 1 cut(s) 403
BspHI TCATGA 1 cut(s) 352
BspLI GGNNCC 1 cut(s) 436
BspPI GGATC 2 cut(s) 429, 442
BspTNI GGTCTC 3 cut(s) 289, 535, 633
BsrI ACTGG 5 cut(s) 391, 484, 1040, 1096, 1103
BssECI CCNNGG 2 cut(s) 401, 787
BssMI GATC 5 cut(s) 13, 145, 434, 1012, 1197
BssNAI GTATAC 1 cut(s) 73
BssT1I CCWWGG 1 cut(s) 787
Bst1107I GTATAC 1 cut(s) 73
Bst2UI CCWGG 1 cut(s) 442
Bst4CI ACNGT 6 cut(s) 52, 229, 533, 833, 1163, 1307
Bst6I CTCTTC 1 cut(s) 745
BstC8I GCNNGC 1 cut(s) 990
BstDEI CTNAG 3 cut(s) 378, 622, 1110
BstDSI CCRYGG 1 cut(s) 401
BstF5I GGATG 7 cut(s) 10, 733, 964, 1017, 1033, 1054, 1108
BstFNI CGCG 1 cut(s) 403
BstHHI GCGC 1 cut(s) 362
BstKTI GATC 5 cut(s) 16, 148, 437, 1015, 1200
BstMAI GTCTC 5 cut(s) 289, 535, 590, 633, 1313
BstMBI GATC 5 cut(s) 13, 145, 434, 1012, 1197
BstMWI GCNNNNNNNGC 7 cut(s) 149, 582, 767, 989, 1115, 1135, 1211
BstNI CCWGG 1 cut(s) 442
BstNSI RCATGY 3 cut(s) 133, 798, 1039
BstSCI CCNGG 1 cut(s) 440
BstSFI CTRYAG 2 cut(s) 762, 1003
BstUI CGCG 1 cut(s) 403
BstV1I GCAGC 4 cut(s) 20, 386, 855, 1118
BstX2I RGATCY 1 cut(s) 434
BstYI RGATCY 1 cut(s) 434
BstZ17I GTATAC 1 cut(s) 73
BsuRI GGCC 2 cut(s) 948, 1053
BtgI CCRYGG 1 cut(s) 401
BtgZI GCGATG 2 cut(s) 81, 1220
BtsCI GGATG 7 cut(s) 10, 733, 964, 1017, 1033, 1054, 1108
BtsIMutI CAGTG 1 cut(s) 145
Cac8I GCNNGC 1 cut(s) 990
CciI TCATGA 1 cut(s) 352
CfoI GCGC 1 cut(s) 362
Cfr42I CCGCGG 1 cut(s) 404
CseI GACGC 1 cut(s) 827
Csp6I GTAC 6 cut(s) 269, 277, 666, 720, 792, 834
CviQI GTAC 6 cut(s) 269, 277, 666, 720, 792, 834
DdeI CTNAG 3 cut(s) 378, 622, 1110
DpnI GATC 5 cut(s) 15, 147, 436, 1014, 1199
DpnII GATC 5 cut(s) 13, 145, 434, 1012, 1197
DraI TTTAAA 1 cut(s) 121
Eam1104I CTCTTC 1 cut(s) 745
EarI CTCTTC 1 cut(s) 745
EciI GGCGGA 1 cut(s) 427
Eco130I CCWWGG 1 cut(s) 787
Eco31I GGTCTC 3 cut(s) 289, 535, 633
Eco57I CTGAAG 1 cut(s) 723
EcoRII CCWGG 1 cut(s) 440
EcoT14I CCWWGG 1 cut(s) 787
EcoT22I ATGCAT 1 cut(s) 1222
ErhI CCWWGG 1 cut(s) 787
Esp3I CGTCTC 2 cut(s) 590, 1313
FalI AAGNNNNNCTT 2 cut(s) 721, 753
FaqI GGGAC 1 cut(s) 181
FblI GTMKAC 2 cut(s) 72, 254
Fnu4HI GCNGC 6 cut(s) 9, 375, 401, 844, 941, 1107
FokI GGATG 7 cut(s) 17, 720, 971, 1004, 1040, 1061, 1115
Fsp4HI GCNGC 6 cut(s) 9, 375, 401, 844, 941, 1107
FspBI CTAG 6 cut(s) 182, 234, 348, 645, 663, 944
GlaI GCGC 1 cut(s) 361
GluI GCNGC 6 cut(s) 9, 375, 401, 844, 941, 1107
HaeIII GGCC 2 cut(s) 948, 1053
HapII CCGG 2 cut(s) 81, 545
HgaI GACGC 1 cut(s) 827
HhaI GCGC 1 cut(s) 362
Hin6I GCGC 1 cut(s) 360
HinP1I GCGC 1 cut(s) 360
HincII GTYRAC 3 cut(s) 221, 255, 477
HindII GTYRAC 3 cut(s) 221, 255, 477
HindIII AAGCTT 1 cut(s) 333
HpaII CCGG 2 cut(s) 81, 545
Hpy166II GTNNAC 6 cut(s) 73, 221, 255, 477, 967, 1033
Hpy188III TCNNGA 6 cut(s) 17, 286, 353, 683, 1195, 1310
Hpy8I GTNNAC 6 cut(s) 73, 221, 255, 477, 967, 1033
Hpy99I CGWCG 2 cut(s) 298, 821
HpyAV CCTTC 6 cut(s) 120, 575, 740, 747, 1064, 1149
HpyCH4III ACNGT 6 cut(s) 52, 229, 533, 833, 1163, 1307
HpyCH4IV ACGT 5 cut(s) 247, 599, 912, 1075, 1286
HpyCH4V TGCA 7 cut(s) 8, 133, 192, 992, 1106, 1220, 1235
HpyF10VI GCNNNNNNNGC 7 cut(s) 149, 582, 767, 989, 1115, 1135, 1211
HpyF3I CTNAG 3 cut(s) 378, 622, 1110
HpySE526I ACGT 5 cut(s) 247, 599, 912, 1075, 1286
HspAI GCGC 1 cut(s) 360
KspI CCGCGG 1 cut(s) 404
Kzo9I GATC 5 cut(s) 13, 145, 434, 1012, 1197
Lsp1109I GCAGC 4 cut(s) 20, 386, 855, 1118
LweI GCATC 2 cut(s) 53, 1093
MaeI CTAG 6 cut(s) 182, 234, 348, 645, 663, 944
MaeII ACGT 5 cut(s) 247, 599, 912, 1075, 1286
MaeIII GTNAC 4 cut(s) 57, 456, 533, 1282
MalI GATC 5 cut(s) 15, 147, 436, 1014, 1199
MboI GATC 5 cut(s) 13, 145, 434, 1012, 1197
MboII GAAGA 5 cut(s) 762, 878, 908, 1097, 1250
MfeI CAATTG 1 cut(s) 197
MflI RGATCY 1 cut(s) 434
MluCI AATT 6 cut(s) 197, 452, 511, 882, 1122, 1295
MlyI GAGTC 4 cut(s) 637, 704, 818, 1256
MmeI TCCRAC 1 cut(s) 276
MnlI CCTC 9 cut(s) 13, 39, 158, 436, 541, 631, 795, 1031, 1143
Mph1103I ATGCAT 1 cut(s) 1222
MseI TTAA 2 cut(s) 120, 909
MslI CAYNNNNRTG 2 cut(s) 1022, 1205
MspA1I CMGCKG 4 cut(s) 377, 403, 743, 1109
MspI CCGG 2 cut(s) 81, 545
MspR9I CCNGG 1 cut(s) 442
MunI CAATTG 1 cut(s) 197
MvaI CCWGG 1 cut(s) 442
MvnI CGCG 1 cut(s) 403
MwoI GCNNNNNNNGC 7 cut(s) 149, 582, 767, 989, 1115, 1135, 1211
NdeII GATC 5 cut(s) 13, 145, 434, 1012, 1197
NlaIV GGNNCC 1 cut(s) 436
NsiI ATGCAT 1 cut(s) 1222
NspI RCATGY 3 cut(s) 133, 798, 1039
PagI TCATGA 1 cut(s) 352
PciI ACATGT 1 cut(s) 794
PfeI GAWTC 6 cut(s) 84, 323, 702, 725, 782, 1191
PflFI GACNNNGTC 1 cut(s) 292
PflMI CCANNNNNTGG 1 cut(s) 955
PkrI GCNGC 6 cut(s) 10, 376, 402, 845, 942, 1108
PleI GAGTC 4 cut(s) 636, 704, 818, 1256
PpsI GAGTC 4 cut(s) 636, 704, 818, 1256
PscI ACATGT 1 cut(s) 794
Psp1406I AACGTT 2 cut(s) 912, 1286
Psp6I CCWGG 1 cut(s) 440
PspGI CCWGG 1 cut(s) 440
PspN4I GGNNCC 1 cut(s) 436
PsrI GAACNNNNNNTAC 3 cut(s) 832, 864, 1305
PsuI RGATCY 1 cut(s) 434
PsyI GACNNNGTC 1 cut(s) 292
PvuII CAGCTG 2 cut(s) 377, 1109
RsaI GTAC 6 cut(s) 270, 278, 667, 721, 793, 835
RsaNI GTAC 6 cut(s) 269, 277, 666, 720, 792, 834
RseI CAYNNNNRTG 2 cut(s) 1022, 1205
SacII CCGCGG 1 cut(s) 404
SalI GTCGAC 1 cut(s) 253
SaqAI TTAA 2 cut(s) 120, 909
SatI GCNGC 6 cut(s) 9, 375, 401, 844, 941, 1107
Sau3AI GATC 5 cut(s) 13, 145, 434, 1012, 1197
ScaI AGTACT 1 cut(s) 835
SchI GAGTC 4 cut(s) 637, 704, 818, 1256
ScrFI CCNGG 1 cut(s) 442
SfaNI GCATC 2 cut(s) 53, 1093
SfcI CTRYAG 2 cut(s) 762, 1003
Sfr303I CCGCGG 1 cut(s) 404
SgrBI CCGCGG 1 cut(s) 404
SmiMI CAYNNNNRTG 2 cut(s) 1022, 1205
Sse9I AATT 6 cut(s) 197, 452, 511, 882, 1122, 1295
SsiI CCGC 7 cut(s) 401, 403, 438, 461, 593, 743, 940
SspMI CTAG 6 cut(s) 182, 234, 348, 645, 663, 944
StyD4I CCNGG 1 cut(s) 440
StyI CCWWGG 1 cut(s) 787
TaaI ACNGT 6 cut(s) 52, 229, 533, 833, 1163, 1307
TaiI ACGT 5 cut(s) 250, 602, 915, 1078, 1289
TaqI TCGA 5 cut(s) 204, 254, 287, 682, 1311
TasI AATT 6 cut(s) 197, 452, 511, 882, 1122, 1295
TatI WGTACW 4 cut(s) 268, 665, 719, 833
TauI GCSGC 2 cut(s) 403, 943
TfiI GAWTC 6 cut(s) 84, 323, 702, 725, 782, 1191
Tru1I TTAA 2 cut(s) 120, 909
Tru9I TTAA 2 cut(s) 120, 909
TscAI CASTG 1 cut(s) 145
TseI GCWGC 4 cut(s) 8, 374, 843, 1106
TspDTI ATGAA 5 cut(s) 384, 504, 950, 1098, 1131
TspGWI ACGGA 1 cut(s) 987
TspRI CASTG 1 cut(s) 145
Tth111I GACNNNGTC 1 cut(s) 292
Van91I CCANNNNNTGG 1 cut(s) 955
XapI RAATTY 2 cut(s) 511, 882
XceI RCATGY 3 cut(s) 133, 798, 1039
XmiI GTMKAC 2 cut(s) 72, 254
XspI CTAG 6 cut(s) 182, 234, 348, 645, 663, 944
ZrmI AGTACT 1 cut(s) 835
Zsp2I ATGCAT 1 cut(s) 1222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.