Rh6AG445400

Histidine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0009350
Physical Location & Seq
Reverse (-)
63262473 .. 63268171
5699 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG445400.1

Sequence Viewer

Length: 3051 bp
ATGGTTTGTGAAATGGAGATCGATAATATTGAAGAAATGGAAATGGAGGAAGTCCTCCCTTCTATGTGGCCTGAGGATATAGATTCCGATAAGCAATTCAACATTGAGAAGCCAGGAAAAGATCAAGACATGTTGGAGGAGGTCACATTCAAAGAGAAACCAACTACAGTCGATTTCAGGCGGCTGTTGGAGCTAACCAAACACACAGATACAGGCTCTTCCCAATTAGCACACCTCGTAAAGCACTGGGAATATAAGCAGGCGAATGTGGTGAGACTTCTTAGAGAAGAACTTGATATCCTCAGCAAGCAAAAGCAGGAAGTTGAGCTCAAGAAATTGGAGATATTGGAAGAGCACCGCTTCGAGTCAGAGAGCTATGGCGGTGACAAACGCCCCATTTCCATTTTGGATGAAGTGTATCAGTTATGGGCTCCGGATGTTCCTCTAAAGAAACTTGATGTTGTTGTGGAGAGTAAGAGAGTCGAGACAGAAGCCGAGTATGACAGTGCTGTGTACTGGAAGCAAAGGGCTATGCATTTGGAGAAGTTGTTGGAGGCAAGTGTTGCGAGAGAGCAGATTCTTGTCGATAAATTGCAGGAAAGCATTAAGAGTATTGAAAGACAGTCATCGCCGGTTGATGAGCTGTCTGAGATTTTGAAAAGAGCAGATAACTTTTTGCATTTTGTTCTTCAGAATGCCCCTGTTGTTATTGGTCATCAGGATAAAGAGTTGCGGTATCGCTTTATCTACAATCACTTCCCAAGTTTGGGAGAGCAGGACATTATAGGGAAAACAGATGTCGAGATTTTCAGTGGGGCTGGAGTTAAGGAATCTCAAGACTTCAAGAGAGAGGTTCTGGAAAAAGGGATACCGGCGAAAAGGGAGATTACATTCGAGACTGAACTGTTTGGGTCAAAGACGTTTTTGATATATGTGGAACCTGTTTTCAGCAAGGCAGGGGAGACAATTGGTATAAACTATATGGGGATGGAGGTGTCTGATCAGGTGAGGAAAAGAGAGAAGATGGCAAAGCTTCGTGAGGAAATAGCCGTCCAAAAGGCCAAGGAAACAGAGCTGAACAAGACAATTCACATAACGGAGGAGACAATGAGAGCAAAACAGATGCTGGCAACAATGTCTCATGAGATAAGGTCTCCTCTTTCTGGGGTTGTCAGCATGGCTGAGATTCTTACTACCACGAAACTTGACCCCGAGCAACGCCAGCTGTTGGAAGTCATGTTATCTTCAGGAGATTTGGTCCTCCAACTGATAAATGACATCCTTGACCTTTCCAAGGTTGAGTCAGGAGTAATGAAGTTGGAGGCTACAAAGTTCCGGCCAAGACAGGTAGTTAGACATGTACTACAGACTGCGGCAGCATCATTGCAGAAGATTTTGACCTTGGAAGGACATGTTATGGAGAATGTTCCTGTGGAGGTCATTGGAGATGTTTTAAGGATTCGTCAAATTCTAACCAACTTGATCAGTAATGCAATCAAGTTTACTCATGAAGGGAAAGTCGGGATAAAACTTTACATGGTAGCGGATCCAACTTCTGGAGATGATGAAAGATGTCTGCAGATGTCGAATGCAGTTCAATCAATGGTTTCAGTGAATGGATTGGATGAAGAGAAATCAGCATCACAAAATTGCTGCAATGGAGAAGGTATTCGTCGCAAAGAACCTTACCAAAATGGTTCTCTTCACGATGAACCTAGCACACCGGTTAAGAGTGAAGCCTCAATGAATGGAGATAAAGAGGACGAACCCCATTCATCTGAAACAACAACAGTGTGGTTACTTTGCGATGTATATGACACAGGAATTGGAATACCAGAAAATGCTTTACCGACCCTTTTTAAAAGATACATGCAAGTGAGTGCTGATCATGCTCGAAAATATGGCGGGACAGGATTAGGACTAGCAATATGCAAACAATTGGTTGAACTAATGGGGGGCCAACTCACTGTGTCTAGCCGAGAACATTGTGGCTCCACATTCACATTTGTATTGCCTTACAAGGTTTCAACATCGTCGGAACATTCTGATGATCCTGATGAGCTCACGGATATCGCTGAATATGACGATGCTGTAACTGATGATGAAGTAGCTGAAAGCTATTTCCAGTTCCAACCACGCACTTTAGGTTCTCTGTTCTCTTCTAATGGCACCGGCAGGACTCAAAAACTGTTACCACATAAACTCAATGGATTATCACAACATGCATACTCGTTTCCATATAATAACATCAGACCAGAGGAGATTACTTCAGTTGAAAATTGCTCTTCTGCAGTCGATGATGTTGCTGAGACATTATCTGAACCAGAGAGTTCAGTAAGTCACATGCCGAATTCCAATAGCGAAACTCCAGCATGTAGGGACAGAAAGGGTCAAGATGACTCAAACAGTCAATCCCAAAATTCTTCAACAGATTCAAATCATCATGCAGAGGCAAGCAGAGAAAATGGTGTAGCAGCAAAGACAAGAGAACCCCAAGGAACGTGTAATAGACAGGAGAAATCAGATGCAGATTCAGAGTGTAGCTCTGGCAACAGTCCAAAAATACCAAAATCAACATCGAATCCTAAGATTCTTCTTGTTGAAGATAACAAGATCAATATTATGGTGGCACAGTCAATGATGAAGCAGTTAGGCCAAAGCTTAGAGGTTGTAAATGACGGAGCTGAAGCTGTGCGTGCAGTTCAACGCTGTAGTTACGATCTGATTCTGATGGATGTTCATATGCCAGTTATGGATGGCCTACAAGCTACAAGAATAATACGTTCTTTCGAAGAAACTGGCAACTGGGAAGCTGCTAAGAAGGCTGGAATTGAGCAATCTGTGCCTTCTTCAAATACACCACAAAACAGCCACGGACATACGCCTTGTAGAAAGCGAATCCCAATCATTGCGATGACAGCAAATGCATTTGCAGAGAGCGCAGAGGAGTGTTTTGCAAATGGTATGGACTCGTTTGTGCCGAAACCTGTAACGCGCCAAAAATTGAAAGAGCGGCTTGAACAATATCTACCCGATTGCAACCTAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000302 GO:0002237 GO:0003674 GO:0003824 GO:0004672 GO:0004673 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009617 GO:0009636 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009755 GO:0009787 GO:0009788 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010104 GO:0010105 GO:0010119 GO:0010646 GO:0010648 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016775 GO:0018106 GO:0018193 GO:0018202 GO:0019538 GO:0022622 GO:0023051 GO:0023052 GO:0023057 GO:0032501 GO:0032502 GO:0032870 GO:0033554 GO:0034599 GO:0034614 GO:0035690 GO:0036211 GO:0042221 GO:0042493 GO:0042542 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046677 GO:0048364 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0070297 GO:0070298 GO:0070301 GO:0070887 GO:0071216 GO:0071219 GO:0071236 GO:0071241 GO:0071310 GO:0071368 GO:0071495 GO:0071704 GO:0071731 GO:0071732 GO:0071944 GO:0090333 GO:0097237 GO:0097366 GO:0099402 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902170 GO:1902531 GO:1902532 GO:1905957 GO:1905958
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HATPase_c HisKA Response_reg

Protein Analysis

1016

Amino Acids

114.03

Weight (kDa)

5.05

Isoelectric Point (pI)

52.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HisKA PF00512 373 - 436 2.1e-19 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 598 - 673 8.1e-20 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Response_reg PF00072 864 - 931 3.8e-13 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 2168
AccB7I CCANNNNNTGG 2 cut(s) 1228, 1556
AccBSI CCGCTC 1 cut(s) 3013
AccII CGCG 1 cut(s) 2995
AccIII TCCGGA 1 cut(s) 433
AciI CCGC 8 cut(s) 181, 358, 381, 733, 1373, 1544, 1905, 3013
AclWI GGATC 3 cut(s) 1541, 1554, 2045
AcoI YGGCCR 1 cut(s) 1337
AcsI RAATTY 3 cut(s) 1467, 2350, 2419
AcuI CTGAAG 4 cut(s) 674, 1230, 2253, 2706
AfaI GTAC 2 cut(s) 515, 1362
AfiI CCNNNNNNNGG 6 cut(s) 766, 767, 1163, 1228, 1294, 1556
AflIII ACRYGT 4 cut(s) 129, 1357, 1411, 2501
AgeI ACCGGT 1 cut(s) 1723
AjnI CCWGG 1 cut(s) 112
AloI GAACNNNNNNTCC 2 cut(s) 282, 314
Alw21I GWGCWC 3 cut(s) 330, 357, 2064
Alw26I GTCTC 8 cut(s) 268, 479, 890, 956, 1097, 1143, 1158, 2303
AlwI GGATC 3 cut(s) 1541, 1554, 2045
AlwNI CAGNNNCTG 1 cut(s) 1126
Ama87I CYCGRG 1 cut(s) 1211
Aor13HI TCCGGA 1 cut(s) 433
AoxI GGCC 6 cut(s) 68, 1059, 1337, 1957, 2653, 2758
ApeKI GCWGC 4 cut(s) 1376, 1653, 2474, 2813
ApoI RAATTY 3 cut(s) 1467, 2350, 2419
AsiGI ACCGGT 1 cut(s) 1723
Asp700I GAANNNNTTC 1 cut(s) 1668
AspLEI GCGC 2 cut(s) 2942, 2997
AspS9I GGNCC 2 cut(s) 1258, 1957
AsuHPI GGTGA 3 cut(s) 283, 395, 1018
AsuII TTCGAA 1 cut(s) 2790
AvaI CYCGRG 1 cut(s) 1211
AvaII GGWCC 1 cut(s) 1258
AxyI CCTNAGG 1 cut(s) 72
BamHI GGATCC 1 cut(s) 1546
BanI GGYRCC 1 cut(s) 2168
BanII GRGCYC 3 cut(s) 330, 433, 2064
Bbv12I GWGCWC 3 cut(s) 330, 357, 2064
BbvCI CCTCAGC 1 cut(s) 302
BbvI GCAGC 4 cut(s) 1388, 1640, 2486, 2800
BccI CCATC 4 cut(s) 982, 1018, 2725, 2750
BceAI ACGGC 1 cut(s) 1034
BcgI CGANNNNNNTGC 2 cut(s) 2285, 2319
BciT130I CCWGG 1 cut(s) 114
BciVI GTATCC 1 cut(s) 861
BclI TGATCA 3 cut(s) 1000, 1482, 1885
BcoDI GTCTC 8 cut(s) 268, 479, 890, 956, 1097, 1143, 1158, 2303
BfaI CTAG 4 cut(s) 1716, 1922, 1974, 3044
BfmI CTRYAG 5 cut(s) 165, 1364, 1577, 2289, 2710
BfuI GTATCC 1 cut(s) 861
BisI GCNGC 7 cut(s) 182, 1374, 1377, 1654, 2475, 2814, 3014
BlsI GCNGC 7 cut(s) 183, 1375, 1378, 1655, 2476, 2815, 3015
Bme1390I CCNGG 1 cut(s) 114
Bme18I GGWCC 1 cut(s) 1258
BmeT110I CYCGRG 1 cut(s) 1211
BmgT120I GGNCC 2 cut(s) 1258, 1957
BmiI GGNNCC 6 cut(s) 432, 939, 1548, 1958, 1993, 2170
BmrFI CCNGG 1 cut(s) 114
BmrI ACTGGG 2 cut(s) 256, 2815
BmsI GCATC 5 cut(s) 1113, 1388, 1649, 2077, 2515
BmuI ACTGGG 2 cut(s) 256, 2815
BplI GAGNNNNNCTC 2 cut(s) 2528, 2560
BpmI CTGGAG 3 cut(s) 840, 1578, 2352
Bpu10I CCTNAGC 1 cut(s) 302
Bpu14I TTCGAA 1 cut(s) 2790
BpuEI CTTGAG 2 cut(s) 314, 819
Bsa29I ATCGAT 1 cut(s) 21
BsaBI GATNNNNATC 1 cut(s) 2436
BsaI GGTCTC 1 cut(s) 1158
BsaJI CCNNGG 5 cut(s) 1062, 1293, 1401, 2494, 2872
BsaWI WCCGGW 2 cut(s) 433, 1723
BsaXI ACNNNNNCTCC 4 cut(s) 533, 563, 1242, 1272
Bsc4I CCNNNNNNNGG 6 cut(s) 766, 767, 1163, 1228, 1294, 1556
Bse118I RCCGGY 4 cut(s) 631, 871, 1723, 2171
Bse1I ACTGG 6 cut(s) 251, 521, 2125, 2747, 2803, 2810
Bse21I CCTNAGG 1 cut(s) 72
Bse3DI GCAATG 3 cut(s) 1382, 1663, 2907
Bse8I GATNNNNATC 1 cut(s) 2436
BseAI TCCGGA 1 cut(s) 433
BseBI CCWGG 1 cut(s) 114
BseCI ATCGAT 1 cut(s) 21
BseDI CCNNGG 5 cut(s) 1062, 1293, 1401, 2494, 2872
BseGI GGATG 7 cut(s) 415, 442, 993, 1278, 1630, 2740, 2761
BseJI GATNNNNATC 1 cut(s) 2436
BseLI CCNNNNNNNGG 6 cut(s) 766, 767, 1163, 1228, 1294, 1556
BseMI GCAATG 3 cut(s) 1382, 1663, 2907
BseMII CTCAG 5 cut(s) 63, 316, 639, 1173, 2298
BseNI ACTGG 6 cut(s) 251, 521, 2125, 2747, 2803, 2810
BseRI GAGGAG 5 cut(s) 152, 1115, 1146, 2273, 2960
BseXI GCAGC 4 cut(s) 1388, 1640, 2486, 2800
BsgI GTGCAG 1 cut(s) 2718
Bsh1236I CGCG 1 cut(s) 2995
BshFI GGCC 6 cut(s) 70, 1061, 1339, 1959, 2655, 2760
BshNI GGYRCC 1 cut(s) 2168
BshTI ACCGGT 1 cut(s) 1723
BshVI ATCGAT 1 cut(s) 21
BsiHKAI GWGCWC 3 cut(s) 330, 357, 2064
BsiHKCI CYCGRG 1 cut(s) 1211
BsiSI CCGG 6 cut(s) 434, 632, 872, 1336, 1724, 2172
BslFI GGGAC 2 cut(s) 1921, 2393
BslI CCNNNNNNNGG 6 cut(s) 766, 767, 1163, 1228, 1294, 1556
BsmAI GTCTC 8 cut(s) 268, 479, 890, 956, 1097, 1143, 1158, 2303
BsmFI GGGAC 2 cut(s) 1921, 2393
BsmI GAATGC 2 cut(s) 700, 1594
BsnI GGCC 6 cut(s) 70, 1061, 1339, 1959, 2655, 2760
Bso31I GGTCTC 1 cut(s) 1158
BsoBI CYCGRG 1 cut(s) 1211
Bsp119I TTCGAA 1 cut(s) 2790
Bsp1286I GDGCHC 4 cut(s) 330, 357, 433, 2064
Bsp13I TCCGGA 1 cut(s) 433
Bsp143I GATC 9 cut(s) 18, 121, 1000, 1482, 1546, 1885, 2050, 2613, 2719
BspACI CCGC 8 cut(s) 181, 358, 381, 733, 1373, 1544, 1905, 3013
BspANI GGCC 6 cut(s) 70, 1061, 1339, 1959, 2655, 2760
BspCNI CTCAG 5 cut(s) 64, 315, 640, 1174, 2299
BspDI ATCGAT 1 cut(s) 21
BspEI TCCGGA 1 cut(s) 433
BspFNI CGCG 1 cut(s) 2995
BspHI TCATGA 2 cut(s) 1141, 1507
BspLI GGNNCC 6 cut(s) 432, 939, 1548, 1958, 1993, 2170
BspMAI CTGCAG 2 cut(s) 1581, 2293
BspPI GGATC 3 cut(s) 1541, 1554, 2045
BspQI GCTCTTC 3 cut(s) 223, 345, 2290
BspT104I TTCGAA 1 cut(s) 2790
BspT107I GGYRCC 1 cut(s) 2168
BspTNI GGTCTC 1 cut(s) 1158
BsrBI CCGCTC 1 cut(s) 3013
BsrDI GCAATG 3 cut(s) 1382, 1663, 2907
BsrFI RCCGGY 4 cut(s) 631, 871, 1723, 2171
BsrI ACTGG 6 cut(s) 251, 521, 2125, 2747, 2803, 2810
BssAI RCCGGY 4 cut(s) 631, 871, 1723, 2171
BssECI CCNNGG 5 cut(s) 1062, 1293, 1401, 2494, 2872
BssMI GATC 9 cut(s) 18, 121, 1000, 1482, 1546, 1885, 2050, 2613, 2719
BssT1I CCWWGG 4 cut(s) 1062, 1293, 1401, 2494
Bst2UI CCWGG 1 cut(s) 114
Bst6I CTCTTC 6 cut(s) 223, 345, 1623, 1707, 2164, 2290
BstAPI GCANNNNNTGC 2 cut(s) 563, 2842
BstBI TTCGAA 1 cut(s) 2790
BstC8I GCNNGC 6 cut(s) 261, 308, 1128, 1223, 2455, 2697
BstDEI CTNAG 9 cut(s) 72, 281, 302, 648, 1182, 2307, 2586, 2662, 2817
BstDSI CCRYGG 1 cut(s) 2872
BstENI CCTNNNNNAGG 1 cut(s) 1292
BstF5I GGATG 7 cut(s) 415, 442, 993, 1278, 1630, 2740, 2761
BstFNI CGCG 1 cut(s) 2995
BstHHI GCGC 2 cut(s) 2942, 2997
BstKTI GATC 9 cut(s) 21, 124, 1003, 1485, 1549, 1888, 2053, 2616, 2722
BstMAI GTCTC 8 cut(s) 268, 479, 890, 956, 1097, 1143, 1158, 2303
BstMBI GATC 9 cut(s) 18, 121, 1000, 1482, 1546, 1885, 2050, 2613, 2719
BstMWI GCNNNNNNNGC 9 cut(s) 190, 563, 1222, 1889, 2696, 2822, 2842, 2918, 2939
BstNI CCWGG 1 cut(s) 114
BstNSI RCATGY 7 cut(s) 133, 1361, 1415, 1873, 2225, 2347, 2376
BstSCI CCNGG 1 cut(s) 112
BstSFI CTRYAG 5 cut(s) 165, 1364, 1577, 2289, 2710
BstUI CGCG 1 cut(s) 2995
BstV1I GCAGC 4 cut(s) 1388, 1640, 2486, 2800
BstX2I RGATCY 1 cut(s) 1546
BstYI RGATCY 1 cut(s) 1546
Bsu15I ATCGAT 1 cut(s) 21
Bsu36I CCTNAGG 1 cut(s) 72
BsuI GTATCC 1 cut(s) 861
BsuRI GGCC 6 cut(s) 70, 1061, 1339, 1959, 2655, 2760
BsuTUI ATCGAT 1 cut(s) 21
BtgI CCRYGG 1 cut(s) 2872
BtgZI GCGATG 3 cut(s) 612, 1821, 2927
BtsCI GGATG 7 cut(s) 415, 442, 993, 1278, 1630, 2740, 2761
BtsIMutI CAGTG 6 cut(s) 244, 511, 817, 1617, 1797, 1965
Cac8I GCNNGC 6 cut(s) 261, 308, 1128, 1223, 2455, 2697
CaiI CAGNNNCTG 1 cut(s) 1126
CciI TCATGA 2 cut(s) 1141, 1507
CfoI GCGC 2 cut(s) 2942, 2997
Cfr10I RCCGGY 4 cut(s) 631, 871, 1723, 2171
Cfr13I GGNCC 2 cut(s) 1258, 1957
ClaI ATCGAT 1 cut(s) 21
Csp6I GTAC 2 cut(s) 514, 1361
CspAI ACCGGT 1 cut(s) 1723
CspCI CAANNNNNGTGG 2 cut(s) 1186, 1221
CviQI GTAC 2 cut(s) 514, 1361
DdeI CTNAG 9 cut(s) 72, 281, 302, 648, 1182, 2307, 2586, 2662, 2817
DpnI GATC 9 cut(s) 20, 123, 1002, 1484, 1548, 1887, 2052, 2615, 2721
DpnII GATC 9 cut(s) 18, 121, 1000, 1482, 1546, 1885, 2050, 2613, 2719
DraI TTTAAA 1 cut(s) 1861
EaeI YGGCCR 1 cut(s) 1337
Eam1104I CTCTTC 6 cut(s) 223, 345, 1623, 1707, 2164, 2290
EarI CTCTTC 6 cut(s) 223, 345, 1623, 1707, 2164, 2290
Ecl136II GAGCTC 2 cut(s) 328, 2062
Eco130I CCWWGG 4 cut(s) 1062, 1293, 1401, 2494
Eco24I GRGCYC 3 cut(s) 330, 433, 2064
Eco31I GGTCTC 1 cut(s) 1158
Eco32I GATATC 2 cut(s) 298, 2071
Eco47I GGWCC 1 cut(s) 1258
Eco53kI GAGCTC 2 cut(s) 328, 2062
Eco57I CTGAAG 4 cut(s) 674, 1230, 2253, 2706
Eco81I CCTNAGG 1 cut(s) 72
Eco88I CYCGRG 1 cut(s) 1211
EcoICRI GAGCTC 2 cut(s) 328, 2062
EcoNI CCTNNNNNAGG 1 cut(s) 1292
EcoRI GAATTC 1 cut(s) 2350
EcoRII CCWGG 1 cut(s) 112
EcoRV GATATC 2 cut(s) 298, 2071
EcoT14I CCWWGG 4 cut(s) 1062, 1293, 1401, 2494
EcoT22I ATGCAT 3 cut(s) 537, 2227, 2929
EcoT38I GRGCYC 3 cut(s) 330, 433, 2064
ErhI CCWWGG 4 cut(s) 1062, 1293, 1401, 2494
FalI AAGNNNNNCTT 2 cut(s) 3000, 3032
FaqI GGGAC 2 cut(s) 1921, 2393
FauI CCCGC 1 cut(s) 1898
FauNDI CATATG 1 cut(s) 2742
FbaI TGATCA 3 cut(s) 1000, 1482, 1885
Fnu4HI GCNGC 7 cut(s) 182, 1374, 1377, 1654, 2475, 2814, 3014
FokI GGATG 7 cut(s) 422, 449, 1000, 1265, 1637, 2747, 2768
FriOI GRGCYC 3 cut(s) 330, 433, 2064
Fsp4HI GCNGC 7 cut(s) 182, 1374, 1377, 1654, 2475, 2814, 3014
FspBI CTAG 4 cut(s) 1716, 1922, 1974, 3044
GlaI GCGC 2 cut(s) 2941, 2996
GluI GCNGC 7 cut(s) 182, 1374, 1377, 1654, 2475, 2814, 3014
GsuI CTGGAG 3 cut(s) 840, 1578, 2352
HaeIII GGCC 6 cut(s) 70, 1061, 1339, 1959, 2655, 2760
HapII CCGG 6 cut(s) 434, 632, 872, 1336, 1724, 2172
HhaI GCGC 2 cut(s) 2942, 2997
Hin6I GCGC 2 cut(s) 2940, 2995
HinP1I GCGC 2 cut(s) 2940, 2995
HindIII AAGCTT 2 cut(s) 1031, 2659
HpaII CCGG 6 cut(s) 434, 632, 872, 1336, 1724, 2172
HphI GGTGA 3 cut(s) 283, 395, 1018
Hpy166II GTNNAC 2 cut(s) 514, 1503
Hpy8I GTNNAC 2 cut(s) 514, 1503
Hpy99I CGWCG 2 cut(s) 1677, 2038
HpyAV CCTTC 6 cut(s) 69, 1400, 1505, 1658, 2815, 2856
HpyCH4IV ACGT 3 cut(s) 920, 2501, 2782
HpyF10VI GCNNNNNNNGC 9 cut(s) 190, 563, 1222, 1889, 2696, 2822, 2842, 2918, 2939
HpyF3I CTNAG 9 cut(s) 72, 281, 302, 648, 1182, 2307, 2586, 2662, 2817
HpySE526I ACGT 3 cut(s) 920, 2501, 2782
HspAI GCGC 2 cut(s) 2940, 2995
Kpn2I TCCGGA 1 cut(s) 433
Ksp22I TGATCA 3 cut(s) 1000, 1482, 1885
Kzo9I GATC 9 cut(s) 18, 121, 1000, 1482, 1546, 1885, 2050, 2613, 2719
LguI GCTCTTC 3 cut(s) 223, 345, 2290
LmnI GCTCC 4 cut(s) 190, 436, 1997, 2681
Lsp1109I GCAGC 4 cut(s) 1388, 1640, 2486, 2800
LweI GCATC 5 cut(s) 1113, 1388, 1649, 2077, 2515
MaeI CTAG 4 cut(s) 1716, 1922, 1974, 3044
MaeII ACGT 3 cut(s) 920, 2501, 2782
MaeIII GTNAC 8 cut(s) 142, 383, 1797, 2092, 2190, 2339, 2714, 2989
MalI GATC 9 cut(s) 20, 123, 1002, 1484, 1548, 1887, 2052, 2615, 2721
MbiI CCGCTC 1 cut(s) 3013
MboI GATC 9 cut(s) 18, 121, 1000, 1482, 1546, 1885, 2050, 2613, 2719
MfeI CAATTG 2 cut(s) 966, 1937
MflI RGATCY 1 cut(s) 1546
MhlI GDGCHC 4 cut(s) 330, 357, 433, 2064
MlyI GAGTC 6 cut(s) 374, 489, 1310, 2173, 2393, 2963
MmeI TCCRAC 9 cut(s) 114, 168, 531, 1209, 1288, 1299, 1574, 2016, 2155
Mph1103I ATGCAT 3 cut(s) 537, 2227, 2929
MroI TCCGGA 1 cut(s) 433
MroXI GAANNNNTTC 1 cut(s) 1668
MseI TTAA 5 cut(s) 606, 825, 1454, 1728, 1860
MslI CAYNNNNRTG 3 cut(s) 1874, 2046, 2912
MspA1I CMGCKG 1 cut(s) 1225
MspI CCGG 6 cut(s) 434, 632, 872, 1336, 1724, 2172
MspR9I CCNGG 1 cut(s) 114
MunI CAATTG 2 cut(s) 966, 1937
Mva1269I GAATGC 2 cut(s) 700, 1594
MvaI CCWGG 1 cut(s) 114
MvnI CGCG 1 cut(s) 2995
MwoI GCNNNNNNNGC 9 cut(s) 190, 563, 1222, 1889, 2696, 2822, 2842, 2918, 2939
NdeI CATATG 1 cut(s) 2742
NdeII GATC 9 cut(s) 18, 121, 1000, 1482, 1546, 1885, 2050, 2613, 2719
NlaIV GGNNCC 6 cut(s) 432, 939, 1548, 1958, 1993, 2170
NmeAIII GCCGAG 2 cut(s) 520, 2003
NmuCI GTSAC 3 cut(s) 142, 383, 2339
NsiI ATGCAT 3 cut(s) 537, 2227, 2929
NspI RCATGY 7 cut(s) 133, 1361, 1415, 1873, 2225, 2347, 2376
NspV TTCGAA 1 cut(s) 2790
PagI TCATGA 2 cut(s) 1141, 1507
PciI ACATGT 3 cut(s) 129, 1357, 1411
PciSI GCTCTTC 3 cut(s) 223, 345, 2290
PcsI WCGNNNNNNNCGW 1 cut(s) 2978
PctI GAATGC 2 cut(s) 700, 1594
PdmI GAANNNNTTC 1 cut(s) 1668
PflMI CCANNNNNTGG 2 cut(s) 1228, 1556
PinAI ACCGGT 1 cut(s) 1723
PkrI GCNGC 7 cut(s) 183, 1375, 1378, 1655, 2476, 2815, 3015
PleI GAGTC 6 cut(s) 373, 488, 1309, 2173, 2393, 2963
PpsI GAGTC 6 cut(s) 373, 488, 1309, 2173, 2393, 2963
PscI ACATGT 3 cut(s) 129, 1357, 1411
Psp124BI GAGCTC 2 cut(s) 330, 2064
Psp6I CCWGG 1 cut(s) 112
PspGI CCWGG 1 cut(s) 112
PspN4I GGNNCC 6 cut(s) 432, 939, 1548, 1958, 1993, 2170
PspPI GGNCC 2 cut(s) 1258, 1957
PsrI GAACNNNNNNTAC 2 cut(s) 3012, 3044
PstI CTGCAG 2 cut(s) 1581, 2293
PstNI CAGNNNCTG 1 cut(s) 1126
PsuI RGATCY 1 cut(s) 1546
PvuII CAGCTG 1 cut(s) 1225
RsaI GTAC 2 cut(s) 515, 1362
RsaNI GTAC 2 cut(s) 514, 1361
RseI CAYNNNNRTG 3 cut(s) 1874, 2046, 2912
SacI GAGCTC 2 cut(s) 330, 2064
SapI GCTCTTC 3 cut(s) 223, 345, 2290
SaqAI TTAA 5 cut(s) 606, 825, 1454, 1728, 1860
SatI GCNGC 7 cut(s) 182, 1374, 1377, 1654, 2475, 2814, 3014
Sau3AI GATC 9 cut(s) 18, 121, 1000, 1482, 1546, 1885, 2050, 2613, 2719
Sau96I GGNCC 2 cut(s) 1258, 1957
SchI GAGTC 6 cut(s) 374, 489, 1310, 2173, 2393, 2963
ScrFI CCNGG 1 cut(s) 114
SduI GDGCHC 4 cut(s) 330, 357, 433, 2064
SfaNI GCATC 5 cut(s) 1113, 1388, 1649, 2077, 2515
SfcI CTRYAG 5 cut(s) 165, 1364, 1577, 2289, 2710
SfuI TTCGAA 1 cut(s) 2790
SinI GGWCC 1 cut(s) 1258
SmiMI CAYNNNNRTG 3 cut(s) 1874, 2046, 2912
SmlI CTYRAG 2 cut(s) 329, 834
SmoI CTYRAG 2 cut(s) 329, 834
SsiI CCGC 8 cut(s) 181, 358, 381, 733, 1373, 1544, 1905, 3013
SspI AATATT 2 cut(s) 28, 2620
SspMI CTAG 4 cut(s) 1716, 1922, 1974, 3044
SstI GAGCTC 2 cut(s) 330, 2064
StyD4I CCNGG 1 cut(s) 112
StyI CCWWGG 4 cut(s) 1062, 1293, 1401, 2494
TaiI ACGT 3 cut(s) 923, 2504, 2785
TatI WGTACW 2 cut(s) 513, 1360
TauI GCSGC 3 cut(s) 184, 1376, 3016
Tru1I TTAA 5 cut(s) 606, 825, 1454, 1728, 1860
Tru9I TTAA 5 cut(s) 606, 825, 1454, 1728, 1860
TscAI CASTG 6 cut(s) 251, 511, 817, 1617, 1797, 1972
TseFI GTSAC 3 cut(s) 142, 383, 2339
TseI GCWGC 4 cut(s) 1376, 1653, 2474, 2813
Tsp45I GTSAC 3 cut(s) 142, 383, 2339
TspGWI ACGGA 4 cut(s) 1112, 2081, 2694, 2889
TspRI CASTG 6 cut(s) 251, 511, 817, 1617, 1797, 1972
Van91I CCANNNNNTGG 2 cut(s) 1228, 1556
VpaK11BI GGWCC 1 cut(s) 1258
XagI CCTNNNNNAGG 1 cut(s) 1292
XapI RAATTY 3 cut(s) 1467, 2350, 2419
XceI RCATGY 7 cut(s) 133, 1361, 1415, 1873, 2225, 2347, 2376
XcmI CCANNNNNNNNNTGG 1 cut(s) 403
XmnI GAANNNNTTC 1 cut(s) 1668
XspI CTAG 4 cut(s) 1716, 1922, 1974, 3044
Zsp2I ATGCAT 3 cut(s) 537, 2227, 2929
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.