MD15G1046500.v1.1

Histidine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009350
Physical Location & Seq
Reverse (-)
3187188 .. 3192778
5591 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1046500.v1.1.491

Sequence Viewer

Length: 3057 bp
ATGGTTTGGGATATGGAGACCGACAACATCGACGACATGGACATGGAAGTCCTCCGTTCCATGTGGCCTGACGACATCGGAGCTGATGGCGGAAAGCAATTCAACATAGACAACCCGGGGCGAGATGAGGACATGTTGGAGGAGGTGACATTCCTAGAGGAACCAACAATCGTTGATTTTCAGCGTCTTGTCGAGCTGACAAACTACACCGATAAGGGCTCTTCGCAACTTGCATTCCTCGTAAAACACTGGGAGTACAAGCAGGCAAACGCCGTGCGCCTTCTCAGAGAAGAACTCGACAGCTTAAGCAAGCAGAGGCAGGAAGTTGAGCTCAAGAAGTTGGAGATATTGGAAGACCACCTCTTTGAGTCAGAGAGTTATGGAGGTGACAAGCGCCCCATTTCGATTTTGGATGAAGTATATGAGATATGGCCGGAAATTGCCCCGAAGAAACATGATGCTGTTGTGCAGACAAAGAAGGTTGAGGTAGAACCCGAGTATGATACTGTTGCGTACTGGAAGCAGAGGGCAATGCAATTGGAGAAAGTGTTGGAGGCGAGCGCCGAGAGAGAGCAGATTCTTGTGGAAAGATTGCAGGAAAGCATTCAGTCTCTGGAAAGGCAGTCATCGCCGGTTGAAGAACTGTCTCAGATTTTGAAAAGAGCTGATAATTTCTTGCATTTTGTTCTTCAGAATGCACCTGTTGTTATCGGCCACCAGGATAAAGAATTGCGGTATCGGTTTATCTATAATCACTTCCCAAGTTTGCAAGAGGAGGACATTATAGGAAGAACAGATGTTGAAATTTTTTCCGGGGCCGGCGTTCAAGAAGCTCAGGATTTCAAGAAAGAGGTTCTTGAGAAAGGCATACCTGCAAAACGGGAGATGACATTTGAAACGCCGTTGTTCGGGACAAAAAAGTTTTTGATATATGTGGAACCTGTCTTCAGCAAGGCAGGAGAAACAATTGGTATAAATTACATGGGGATGGATGTCACTGATCAGGTGAGGAAAAGAGAGAAGATGGCGAAGCTTCGAGAAGAAATCGCAGTCCAGAAAGCCAAGGAAACAGAACTGAACAAAACAATTCACATCACAGAGGAGACAATGCGAGCGAAACAGATGCTGGCAACAATGTCTCATGAGATAAGATCGCCTCTTTCCGGGGTTGTTAGCATGGCTGAGATTCTCACCACCACGAAACTTGATCGCGAGCAACGCCAGCTTTTGGACGTCATGTTATCTTCGGGAGATTTGGTCCTTCAACTGATAAACGACATCCTTGACCTTTCCAAGGTCGAGTCAGGAGTAATGAAGTTGGAAGCTACGAAATTCCGGCCACGAGAGGTAGCAAGGCACGTACTACAGACTGCGGCAGCATCATTGCAGAAAATTTTGACCCTGGAAGGGCATGTATCTGAGGATGTTCCTGTGGAGGTCATCGGCGATGTTTTAAGAATTCGTCAAATCCTAACCAATTTGATCAGTAATGCAATCAAGTTTACTCATGAAGGCAAAGTTGGGATAAAACTTTACGTGGTGTCAAATCCGTCTTTTCAAGAGGACGAAGGATGCCCTCAAAACCCGAATACAGTTCAATCGACGGTTTCAGAGAATGGACCGGAAGCAGAGAAACCAGCATCTCAAACTTCTTGTGACCAAGAAGGTTTCGATGGAGACCGACCACACCAAAATCACTCCCTTAACGACGAACCTAGAACCCCGACTAAGCGTGAAGTTTCGATGGATGCAGCAGATCAAGAGGATGAACCTCGTTTGCCCGAAACGAAAACGGTTTGGTTACGATGTGATGTCTACGACACCGGAATCGGAATACCGGAAAATGCTTTACCTTCTCTGTTCAAAAGATACATGCAAGTCAGTGCTGATCATGCTCGAAAATATGGAGGGACAGGATTAGGACTAGCAATATGCAAACAACTGGTTGAGCTAATGGGGGGTCAGCTCACAGTATCTAGCAAAGAAAAATGTGGGTCGACATTTACGTTTGTACTACCGTACAAGGTTTCGACATCATCCGAAAAACATTCCGACGACCCCGATGATTCTGATGAAGCGTCGGATATGGCGAGTCAAGACGGTGCAACTGATGAAGTAGCCGACAGCTATTTCCAGTTCCAGCCGCGAACTTTAGGTTCTCTGTTCTCTTCTAATGGAGCTGCCAGGACCCAAAACTTGGGGTTTACCGGTTCACATAAACTCGATGGGTTTGCACAAAACTCATACTCGTTTCCAAACGGTAGCATCAGATCGGAGGAGATAGATTTGGTTGAAAACTCTTGCCCAGAAGGTGATTTTGCTGAGACATTATCGGAACCAAAGCGCTCCTCAGCAAGCCACATCCGAGATCCAAATAGTGAAAATCCAGCTAGTACAAACAAACAGGGTCGAGGTGACATGTATAATCGATTCCAAGACTCTACAAGTACAAATTCAAGTCATCCTTCAGAGGAAAGAAGAGAAACAGCTGTAGCAGCACCCACAAGAGAACCCCAAGGAACATGCAAGAGGCAGGAGAAACCCGACGCAGACTCAGAGAGTACTAGCAGCAGTTCAAATTTACCAAAACCAACATCGAAACCTAGGATTCTTCTTGTTGAAGATAACAAGATTAACGTTATGGTGGCACAGAACATGATGAAACGGTTAGGCCATAGCATTGATGTTGTGTACAATGGAATGGAAGCTGTGCGTGCCGTTCAACGCTGTAGATACAATCTCATCCTGATGGATGTTCACATGCCAGTTATGGATGGCCTTCAAACTACAAGAATTATACGTTCTTTCGAAGAAACCGGCAACTGGGATGCAGCGGAGAAAGCTGGAATTGAGCTTCCTCTGCCTTCATCAGAAACATTACAGAACGGTCATGGGTACACGCCTTCTAGAAAGCGAATCCCCATCATAGCGATGACAGCAAATGCATTTTCAGAGAGTGCAGAAGAATGTTTCAAAAGTGGAATGGACTCATTCGTGCCAAAGCCCGTAACATTCCAAAAATTGAAGGACCGTCTCGAACAATACCTACCCGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000302 GO:0002237 GO:0003674 GO:0003824 GO:0004672 GO:0004673 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009617 GO:0009636 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009755 GO:0009787 GO:0009788 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010104 GO:0010105 GO:0010119 GO:0010646 GO:0010648 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016775 GO:0018106 GO:0018193 GO:0018202 GO:0019538 GO:0022622 GO:0023051 GO:0023052 GO:0023057 GO:0032501 GO:0032502 GO:0032870 GO:0033554 GO:0034599 GO:0034614 GO:0035690 GO:0036211 GO:0042221 GO:0042493 GO:0042542 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046677 GO:0048364 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0070297 GO:0070298 GO:0070301 GO:0070887 GO:0071216 GO:0071219 GO:0071236 GO:0071241 GO:0071310 GO:0071368 GO:0071495 GO:0071704 GO:0071731 GO:0071732 GO:0071944 GO:0090333 GO:0097237 GO:0097366 GO:0099402 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902170 GO:1902531 GO:1902532 GO:1905957 GO:1905958
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HATPase_c HisKA Response_reg

Protein Analysis

1019

Amino Acids

114.61

Weight (kDa)

4.96

Isoelectric Point (pI)

50.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HisKA PF00512 373 - 436 1.9e-18 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 598 - 674 2.2e-20 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Response_reg PF00072 870 - 937 6.5e-13 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 47
AatII GACGTC 1 cut(s) 1236
Acc36I ACCTGC 1 cut(s) 880
AccB7I CCANNNNNTGG 2 cut(s) 1228, 2197
AccI GTMKAC 2 cut(s) 1815, 1997
AccII CGCG 2 cut(s) 1212, 2146
AciI CCGC 5 cut(s) 90, 733, 1373, 2144, 2834
AclI AACGTT 1 cut(s) 2637
AclWI GGATC 1 cut(s) 2363
AcoI YGGCCR 3 cut(s) 431, 712, 1337
AcsI RAATTY 6 cut(s) 804, 1331, 1392, 1458, 2452, 2578
AcuI CTGAAG 3 cut(s) 674, 931, 2451
AcyI GRCGYC 1 cut(s) 1233
AfeI AGCGCT 1 cut(s) 2345
AfiI CCNNNNNNNGG 7 cut(s) 908, 1163, 1228, 1294, 1407, 2197, 2823
AflII CTTAAG 1 cut(s) 304
AflIII ACRYGT 2 cut(s) 132, 2418
AgeI ACCGGT 1 cut(s) 2207
AjnI CCWGG 3 cut(s) 717, 1401, 2183
AjuI GAANNNNNNNTTGG 2 cut(s) 1503, 1535
Alw21I GWGCWC 1 cut(s) 333
Alw26I GTCTC 8 cut(s) 11, 615, 651, 1097, 1143, 1671, 2318, 3038
AlwI GGATC 1 cut(s) 2363
AlwNI CAGNNNCTG 2 cut(s) 613, 1126
Ama87I CYCGRG 2 cut(s) 115, 494
Aor51HI AGCGCT 1 cut(s) 2345
AoxI GGCC 7 cut(s) 65, 431, 712, 816, 1337, 2671, 2776
ApeKI GCWGC 6 cut(s) 1376, 1751, 2180, 2495, 2568, 2831
ApoI RAATTY 6 cut(s) 804, 1331, 1392, 1458, 2452, 2578
ArsI GACNNNNNNTTYG 2 cut(s) 347, 379
AsiGI ACCGGT 1 cut(s) 2207
Asp700I GAANNNNTTC 1 cut(s) 603
AspA2I CCTAGG 1 cut(s) 2603
AspLEI GCGC 4 cut(s) 279, 396, 563, 2346
AspS9I GGNCC 5 cut(s) 816, 1258, 1619, 2187, 3028
AsuC2I CCSGG 4 cut(s) 116, 117, 814, 1165
AsuHPI GGTGA 6 cut(s) 157, 398, 1018, 1183, 2324, 2426
AsuII TTCGAA 1 cut(s) 2808
AvaI CYCGRG 2 cut(s) 115, 494
AvaII GGWCC 4 cut(s) 1258, 1619, 2187, 3028
AvrII CCTAGG 1 cut(s) 2603
BanII GRGCYC 2 cut(s) 221, 333
BauI CACGAG 1 cut(s) 1341
BbsI GAAGAC 2 cut(s) 360, 937
Bbv12I GWGCWC 1 cut(s) 333
BbvCI CCTCAGC 1 cut(s) 2350
BbvI GCAGC 6 cut(s) 1388, 1763, 2167, 2507, 2580, 2843
BccI CCATC 9 cut(s) 80, 982, 1018, 1667, 1738, 2219, 2743, 2768, 2930
BceAI ACGGC 3 cut(s) 257, 886, 2702
BcgI CGANNNNNNTGC 4 cut(s) 1105, 1139, 2213, 2247
BciT130I CCWGG 3 cut(s) 719, 1403, 2185
BclI TGATCA 3 cut(s) 1000, 1482, 1888
BcnI CCSGG 4 cut(s) 116, 117, 814, 1165
BcoDI GTCTC 8 cut(s) 11, 615, 651, 1097, 1143, 1671, 2318, 3038
BfaI CTAG 8 cut(s) 155, 1716, 1925, 1977, 2391, 2565, 2604, 2907
BfmI CTRYAG 3 cut(s) 1364, 2490, 2728
BfoI RGCGCY 3 cut(s) 397, 564, 2347
BfrI CTTAAG 1 cut(s) 304
BfuAI ACCTGC 1 cut(s) 880
BisI GCNGC 8 cut(s) 1374, 1377, 1752, 2144, 2181, 2496, 2569, 2832
BlnI CCTAGG 1 cut(s) 2603
BlsI GCNGC 8 cut(s) 1375, 1378, 1753, 2145, 2182, 2497, 2570, 2833
BmcAI AGTACT 1 cut(s) 2563
Bme1390I CCNGG 7 cut(s) 116, 117, 719, 814, 1165, 1403, 2185
Bme18I GGWCC 4 cut(s) 1258, 1619, 2187, 3028
BmeT110I CYCGRG 2 cut(s) 115, 494
BmgT120I GGNCC 5 cut(s) 816, 1258, 1619, 2187, 3028
BmiI GGNNCC 5 cut(s) 162, 817, 939, 2189, 2337
BmrFI CCNGG 7 cut(s) 116, 117, 719, 814, 1165, 1403, 2185
BmrI ACTGGG 2 cut(s) 259, 2833
BmsI GCATC 8 cut(s) 448, 1113, 1388, 1562, 1649, 1738, 2274, 2818
BmuI ACTGGG 2 cut(s) 259, 2833
BpiI GAAGAC 2 cut(s) 360, 937
BplI GAGNNNNNCTC 2 cut(s) 279, 311
Bpu10I CCTNAGC 2 cut(s) 834, 2350
Bpu14I TTCGAA 1 cut(s) 2808
BpuEI CTTGAG 2 cut(s) 317, 878
BpuMI CCSGG 4 cut(s) 116, 117, 814, 1165
Bsa29I ATCGAT 1 cut(s) 2428
BsaAI YACGTR 2 cut(s) 1360, 1537
BsaHI GRCGYC 1 cut(s) 1233
BsaI GGTCTC 2 cut(s) 11, 1671
BsaJI CCNNGG 9 cut(s) 115, 116, 813, 1062, 1164, 1293, 1401, 2515, 2603
BsaWI WCCGGW 4 cut(s) 1621, 1823, 1837, 2207
BsaXI ACNNNNNCTCC 4 cut(s) 533, 563, 1242, 1272
Bsc4I CCNNNNNNNGG 7 cut(s) 908, 1163, 1228, 1294, 1407, 2197, 2823
Bse118I RCCGGY 4 cut(s) 631, 818, 2207, 2816
Bse1I ACTGG 6 cut(s) 254, 521, 1947, 2134, 2765, 2828
Bse3DI GCAATG 2 cut(s) 537, 1382
BseBI CCWGG 3 cut(s) 719, 1403, 2185
BseCI ATCGAT 1 cut(s) 2428
BseDI CCNNGG 9 cut(s) 115, 116, 813, 1062, 1164, 1293, 1401, 2515, 2603
BseLI CCNNNNNNNGG 7 cut(s) 908, 1163, 1228, 1294, 1407, 2197, 2823
BseMI GCAATG 2 cut(s) 537, 1382
BseMII CTCAG 8 cut(s) 298, 662, 848, 1173, 1410, 2313, 2364, 2568
BseNI ACTGG 6 cut(s) 254, 521, 1947, 2134, 2765, 2828
BseRI GAGGAG 5 cut(s) 155, 788, 1115, 2291, 2338
BseXI GCAGC 6 cut(s) 1388, 1763, 2167, 2507, 2580, 2843
BsgI GTGCAG 2 cut(s) 488, 2979
Bsh1236I CGCG 2 cut(s) 1212, 2146
BshFI GGCC 7 cut(s) 67, 433, 714, 818, 1339, 2673, 2778
BshTI ACCGGT 1 cut(s) 2207
BshVI ATCGAT 1 cut(s) 2428
BsiHKAI GWGCWC 1 cut(s) 333
BsiHKCI CYCGRG 2 cut(s) 115, 494
BslFI GGGAC 2 cut(s) 925, 1924
BslI CCNNNNNNNGG 7 cut(s) 908, 1163, 1228, 1294, 1407, 2197, 2823
BsmAI GTCTC 8 cut(s) 11, 615, 651, 1097, 1143, 1671, 2318, 3038
BsmBI CGTCTC 1 cut(s) 3038
BsmFI GGGAC 2 cut(s) 925, 1924
BsmI GAATGC 3 cut(s) 233, 603, 700
BsnI GGCC 7 cut(s) 67, 433, 714, 818, 1339, 2673, 2778
Bso31I GGTCTC 2 cut(s) 11, 1671
BsoBI CYCGRG 2 cut(s) 115, 494
Bsp119I TTCGAA 1 cut(s) 2808
Bsp1286I GDGCHC 2 cut(s) 221, 333
Bsp1407I TGTACA 1 cut(s) 2691
Bsp143I GATC 8 cut(s) 1000, 1151, 1207, 1482, 1756, 1888, 2270, 2368
Bsp68I TCGCGA 1 cut(s) 1212
BspACI CCGC 5 cut(s) 90, 733, 1373, 2144, 2834
BspANI GGCC 7 cut(s) 67, 433, 714, 818, 1339, 2673, 2778
BspCNI CTCAG 8 cut(s) 297, 661, 847, 1174, 1411, 2314, 2363, 2567
BspDI ATCGAT 1 cut(s) 2428
BspFNI CGCG 2 cut(s) 1212, 2146
BspHI TCATGA 2 cut(s) 1141, 1507
BspLI GGNNCC 5 cut(s) 162, 817, 939, 2189, 2337
BspMI ACCTGC 1 cut(s) 880
BspPI GGATC 1 cut(s) 2363
BspQI GCTCTTC 1 cut(s) 226
BspT104I TTCGAA 1 cut(s) 2808
BspTI CTTAAG 1 cut(s) 304
BspTNI GGTCTC 2 cut(s) 11, 1671
BsrDI GCAATG 2 cut(s) 537, 1382
BsrFI RCCGGY 4 cut(s) 631, 818, 2207, 2816
BsrGI TGTACA 1 cut(s) 2691
BsrI ACTGG 6 cut(s) 254, 521, 1947, 2134, 2765, 2828
BssAI RCCGGY 4 cut(s) 631, 818, 2207, 2816
BssECI CCNNGG 9 cut(s) 115, 116, 813, 1062, 1164, 1293, 1401, 2515, 2603
BssMI GATC 8 cut(s) 1000, 1151, 1207, 1482, 1756, 1888, 2270, 2368
BssNI GRCGYC 1 cut(s) 1233
BssSI CACGAG 1 cut(s) 1341
BssT1I CCWWGG 4 cut(s) 1062, 1293, 2515, 2603
Bst2BI CACGAG 1 cut(s) 1341
Bst2UI CCWGG 3 cut(s) 719, 1403, 2185
Bst6I CTCTTC 3 cut(s) 226, 2173, 2473
BstACI GRCGYC 1 cut(s) 1233
BstAFI CTTAAG 1 cut(s) 304
BstAUI TGTACA 1 cut(s) 2691
BstBAI YACGTR 2 cut(s) 1360, 1537
BstBI TTCGAA 1 cut(s) 2808
BstDEI CTNAG 9 cut(s) 284, 648, 834, 1182, 1419, 1728, 2322, 2350, 2554
BstENI CCTNNNNNAGG 1 cut(s) 1292
BstFNI CGCG 2 cut(s) 1212, 2146
BstH2I RGCGCY 3 cut(s) 397, 564, 2347
BstHHI GCGC 4 cut(s) 279, 396, 563, 2346
BstKTI GATC 8 cut(s) 1003, 1154, 1210, 1485, 1759, 1891, 2273, 2371
BstMAI GTCTC 8 cut(s) 11, 615, 651, 1097, 1143, 1671, 2318, 3038
BstMBI GATC 8 cut(s) 1000, 1151, 1207, 1482, 1756, 1888, 2270, 2368
BstMWI GCNNNNNNNGC 9 cut(s) 628, 1218, 1222, 1892, 2495, 2714, 2840, 2860, 2936
BstNI CCWGG 3 cut(s) 719, 1403, 2185
BstNSI RCATGY 6 cut(s) 136, 1415, 1876, 2422, 2526, 2764
BstSCI CCNGG 7 cut(s) 114, 115, 717, 812, 1163, 1401, 2183
BstSFI CTRYAG 3 cut(s) 1364, 2490, 2728
BstUI CGCG 2 cut(s) 1212, 2146
BstV1I GCAGC 6 cut(s) 1388, 1763, 2167, 2507, 2580, 2843
BstV2I GAAGAC 2 cut(s) 360, 937
BstX2I RGATCY 1 cut(s) 2368
BstYI RGATCY 1 cut(s) 2368
Bsu15I ATCGAT 1 cut(s) 2428
BsuRI GGCC 7 cut(s) 67, 433, 714, 818, 1339, 2673, 2778
BsuTUI ATCGAT 1 cut(s) 2428
BtgZI GCGATG 3 cut(s) 612, 1461, 2945
BtsIMutI CAGTG 3 cut(s) 247, 996, 1888
BtuMI TCGCGA 1 cut(s) 1212
BveI ACCTGC 1 cut(s) 880
CaiI CAGNNNCTG 2 cut(s) 613, 1126
CciI TCATGA 2 cut(s) 1141, 1507
CfoI GCGC 4 cut(s) 279, 396, 563, 2346
Cfr10I RCCGGY 4 cut(s) 631, 818, 2207, 2816
Cfr13I GGNCC 5 cut(s) 816, 1258, 1619, 2187, 3028
Cfr9I CCCGGG 1 cut(s) 115
ClaI ATCGAT 1 cut(s) 2428
CseI GACGC 3 cut(s) 173, 2067, 2555
CspAI ACCGGT 1 cut(s) 2207
CspCI CAANNNNNGTGG 2 cut(s) 1186, 1221
DdeI CTNAG 9 cut(s) 284, 648, 834, 1182, 1419, 1728, 2322, 2350, 2554
DpnI GATC 8 cut(s) 1002, 1153, 1209, 1484, 1758, 1890, 2272, 2370
DpnII GATC 8 cut(s) 1000, 1151, 1207, 1482, 1756, 1888, 2270, 2368
DrdI GACNNNNNNGTC 1 cut(s) 47
DseDI GACNNNNNNGTC 1 cut(s) 47
EaeI YGGCCR 3 cut(s) 431, 712, 1337
Eam1104I CTCTTC 3 cut(s) 226, 2173, 2473
EarI CTCTTC 3 cut(s) 226, 2173, 2473
EciI GGCGGA 1 cut(s) 105
Ecl136II GAGCTC 1 cut(s) 331
Eco130I CCWWGG 4 cut(s) 1062, 1293, 2515, 2603
Eco24I GRGCYC 2 cut(s) 221, 333
Eco31I GGTCTC 2 cut(s) 11, 1671
Eco47I GGWCC 4 cut(s) 1258, 1619, 2187, 3028
Eco47III AGCGCT 1 cut(s) 2345
Eco53kI GAGCTC 1 cut(s) 331
Eco57I CTGAAG 3 cut(s) 674, 931, 2451
Eco88I CYCGRG 2 cut(s) 115, 494
EcoICRI GAGCTC 1 cut(s) 331
EcoNI CCTNNNNNAGG 1 cut(s) 1292
EcoO109I RGGNCCY 1 cut(s) 2187
EcoRI GAATTC 1 cut(s) 1458
EcoRII CCWGG 3 cut(s) 717, 1401, 2183
EcoT14I CCWWGG 4 cut(s) 1062, 1293, 2515, 2603
EcoT22I ATGCAT 1 cut(s) 2947
EcoT38I GRGCYC 2 cut(s) 221, 333
ErhI CCWWGG 4 cut(s) 1062, 1293, 2515, 2603
Esp3I CGTCTC 1 cut(s) 3038
FalI AAGNNNNNCTT 4 cut(s) 840, 872, 2449, 2481
FaqI GGGAC 2 cut(s) 925, 1924
FbaI TGATCA 3 cut(s) 1000, 1482, 1888
FblI GTMKAC 2 cut(s) 1815, 1997
Fnu4HI GCNGC 8 cut(s) 1374, 1377, 1752, 2144, 2181, 2496, 2569, 2832
FriOI GRGCYC 2 cut(s) 221, 333
Fsp4HI GCNGC 8 cut(s) 1374, 1377, 1752, 2144, 2181, 2496, 2569, 2832
FspBI CTAG 8 cut(s) 155, 1716, 1925, 1977, 2391, 2565, 2604, 2907
GlaI GCGC 4 cut(s) 278, 395, 562, 2345
GluI GCNGC 8 cut(s) 1374, 1377, 1752, 2144, 2181, 2496, 2569, 2832
HaeII RGCGCY 3 cut(s) 397, 564, 2347
HaeIII GGCC 7 cut(s) 67, 433, 714, 818, 1339, 2673, 2778
HgaI GACGC 3 cut(s) 173, 2067, 2555
HhaI GCGC 4 cut(s) 279, 396, 563, 2346
Hin1I GRCGYC 1 cut(s) 1233
Hin6I GCGC 4 cut(s) 277, 394, 561, 2344
HinP1I GCGC 4 cut(s) 277, 394, 561, 2344
HincII GTYRAC 1 cut(s) 1998
HindII GTYRAC 1 cut(s) 1998
HindIII AAGCTT 1 cut(s) 1031
HphI GGTGA 6 cut(s) 157, 398, 1018, 1183, 2324, 2426
Hpy166II GTNNAC 8 cut(s) 1503, 1816, 1998, 2205, 2213, 2692, 2758, 2898
Hpy8I GTNNAC 8 cut(s) 1503, 1816, 1998, 2205, 2213, 2692, 2758, 2898
Hpy99I CGWCG 6 cut(s) 35, 1606, 1712, 2057, 2083, 2549
HpyCH4IV ACGT 6 cut(s) 1233, 1359, 1536, 2006, 2637, 2800
HpyF10VI GCNNNNNNNGC 9 cut(s) 628, 1218, 1222, 1892, 2495, 2714, 2840, 2860, 2936
HpyF3I CTNAG 9 cut(s) 284, 648, 834, 1182, 1419, 1728, 2322, 2350, 2554
HpySE526I ACGT 6 cut(s) 1233, 1359, 1536, 2006, 2637, 2800
Hsp92I GRCGYC 1 cut(s) 1233
HspAI GCGC 4 cut(s) 277, 394, 561, 2344
KroI GCCGGC 1 cut(s) 818
KroNI GCCGGC 1 cut(s) 820
Ksp22I TGATCA 3 cut(s) 1000, 1482, 1888
Kzo9I GATC 8 cut(s) 1000, 1151, 1207, 1482, 1756, 1888, 2270, 2368
LguI GCTCTTC 1 cut(s) 226
LmnI GCTCC 3 cut(s) 80, 2177, 2351
Lsp1109I GCAGC 6 cut(s) 1388, 1763, 2167, 2507, 2580, 2843
LweI GCATC 8 cut(s) 448, 1113, 1388, 1562, 1649, 1738, 2274, 2818
MaeI CTAG 8 cut(s) 155, 1716, 1925, 1977, 2391, 2565, 2604, 2907
MaeII ACGT 6 cut(s) 1233, 1359, 1536, 2006, 2637, 2800
MaeIII GTNAC 7 cut(s) 145, 386, 994, 1655, 1800, 2414, 3007
MalI GATC 8 cut(s) 1002, 1153, 1209, 1484, 1758, 1890, 2272, 2370
MboI GATC 8 cut(s) 1000, 1151, 1207, 1482, 1756, 1888, 2270, 2368
MfeI CAATTG 2 cut(s) 536, 966
MflI RGATCY 1 cut(s) 2368
MhlI GDGCHC 2 cut(s) 221, 333
MlyI GAGTC 6 cut(s) 377, 1310, 2101, 2432, 2546, 2981
MmeI TCCRAC 6 cut(s) 117, 321, 531, 1299, 2061, 2076
Mph1103I ATGCAT 1 cut(s) 2947
MroNI GCCGGC 1 cut(s) 818
MroXI GAANNNNTTC 1 cut(s) 603
MseI TTAA 4 cut(s) 305, 1454, 1704, 2634
MslI CAYNNNNRTG 4 cut(s) 41, 986, 2747, 2930
MspA1I CMGCKG 2 cut(s) 2489, 2834
MspCI CTTAAG 1 cut(s) 304
MspR9I CCNGG 7 cut(s) 116, 117, 719, 814, 1165, 1403, 2185
MunI CAATTG 2 cut(s) 536, 966
Mva1269I GAATGC 3 cut(s) 233, 603, 700
MvaI CCWGG 3 cut(s) 719, 1403, 2185
MvnI CGCG 2 cut(s) 1212, 2146
MwoI GCNNNNNNNGC 9 cut(s) 628, 1218, 1222, 1892, 2495, 2714, 2840, 2860, 2936
NaeI GCCGGC 1 cut(s) 820
NciI CCSGG 4 cut(s) 116, 117, 814, 1165
NdeII GATC 8 cut(s) 1000, 1151, 1207, 1482, 1756, 1888, 2270, 2368
NgoMIV GCCGGC 1 cut(s) 818
NlaIV GGNNCC 5 cut(s) 162, 817, 939, 2189, 2337
NmeAIII GCCGAG 1 cut(s) 589
NmuCI GTSAC 5 cut(s) 145, 386, 994, 1655, 2414
NruI TCGCGA 1 cut(s) 1212
NsiI ATGCAT 1 cut(s) 2947
NspI RCATGY 6 cut(s) 136, 1415, 1876, 2422, 2526, 2764
NspV TTCGAA 1 cut(s) 2808
PagI TCATGA 2 cut(s) 1141, 1507
PciI ACATGT 2 cut(s) 132, 2418
PciSI GCTCTTC 1 cut(s) 226
PcsI WCGNNNNNNNCGW 2 cut(s) 2003, 2087
PctI GAATGC 3 cut(s) 233, 603, 700
PdiI GCCGGC 1 cut(s) 820
PdmI GAANNNNTTC 1 cut(s) 603
PfeI GAWTC 7 cut(s) 577, 1186, 1827, 2066, 2430, 2608, 2916
PflMI CCANNNNNTGG 2 cut(s) 1228, 2197
PinAI ACCGGT 1 cut(s) 2207
PkrI GCNGC 8 cut(s) 1375, 1378, 1753, 2145, 2182, 2497, 2570, 2833
PleI GAGTC 6 cut(s) 376, 1309, 2100, 2432, 2546, 2981
PpsI GAGTC 6 cut(s) 376, 1309, 2100, 2432, 2546, 2981
Ppu21I YACGTR 2 cut(s) 1360, 1537
PpuMI RGGWCCY 1 cut(s) 2187
PscI ACATGT 2 cut(s) 132, 2418
Psp124BI GAGCTC 1 cut(s) 333
Psp1406I AACGTT 1 cut(s) 2637
Psp5II RGGWCCY 1 cut(s) 2187
Psp6I CCWGG 3 cut(s) 717, 1401, 2183
PspGI CCWGG 3 cut(s) 717, 1401, 2183
PspN4I GGNNCC 5 cut(s) 162, 817, 939, 2189, 2337
PspPI GGNCC 5 cut(s) 816, 1258, 1619, 2187, 3028
PspPPI RGGWCCY 1 cut(s) 2187
PsrI GAACNNNNNNTAC 1 cut(s) 3030
PstNI CAGNNNCTG 2 cut(s) 613, 1126
PsuI RGATCY 1 cut(s) 2368
PvuII CAGCTG 1 cut(s) 2489
RruI TCGCGA 1 cut(s) 1212
RseI CAYNNNNRTG 4 cut(s) 41, 986, 2747, 2930
SacI GAGCTC 1 cut(s) 333
SalI GTCGAC 1 cut(s) 1996
SapI GCTCTTC 1 cut(s) 226
SaqAI TTAA 4 cut(s) 305, 1454, 1704, 2634
SatI GCNGC 8 cut(s) 1374, 1377, 1752, 2144, 2181, 2496, 2569, 2832
Sau3AI GATC 8 cut(s) 1000, 1151, 1207, 1482, 1756, 1888, 2270, 2368
Sau96I GGNCC 5 cut(s) 816, 1258, 1619, 2187, 3028
ScaI AGTACT 1 cut(s) 2563
SchI GAGTC 6 cut(s) 377, 1310, 2101, 2432, 2546, 2981
ScrFI CCNGG 7 cut(s) 116, 117, 719, 814, 1165, 1403, 2185
SduI GDGCHC 2 cut(s) 221, 333
SfaNI GCATC 8 cut(s) 448, 1113, 1388, 1562, 1649, 1738, 2274, 2818
SfcI CTRYAG 3 cut(s) 1364, 2490, 2728
SfuI TTCGAA 1 cut(s) 2808
SinI GGWCC 4 cut(s) 1258, 1619, 2187, 3028
SmaI CCCGGG 1 cut(s) 117
SmiMI CAYNNNNRTG 4 cut(s) 41, 986, 2747, 2930
SmlI CTYRAG 3 cut(s) 304, 332, 857
SmoI CTYRAG 3 cut(s) 304, 332, 857
SsiI CCGC 5 cut(s) 90, 733, 1373, 2144, 2834
SspMI CTAG 8 cut(s) 155, 1716, 1925, 1977, 2391, 2565, 2604, 2907
SstI GAGCTC 1 cut(s) 333
StyD4I CCNGG 7 cut(s) 114, 115, 717, 812, 1163, 1401, 2183
StyI CCWWGG 4 cut(s) 1062, 1293, 2515, 2603
TaiI ACGT 6 cut(s) 1236, 1362, 1539, 2009, 2640, 2803
TaqII GACCGA 2 cut(s) 35, 1695
TatI WGTACW 6 cut(s) 255, 2011, 2393, 2447, 2561, 2691
TauI GCSGC 2 cut(s) 1376, 2146
TfiI GAWTC 7 cut(s) 577, 1186, 1827, 2066, 2430, 2608, 2916
Tru1I TTAA 4 cut(s) 305, 1454, 1704, 2634
Tru9I TTAA 4 cut(s) 305, 1454, 1704, 2634
TscAI CASTG 3 cut(s) 254, 1003, 1888
TseFI GTSAC 5 cut(s) 145, 386, 994, 1655, 2414
TseI GCWGC 6 cut(s) 1376, 1751, 2180, 2495, 2568, 2831
Tsp45I GTSAC 5 cut(s) 145, 386, 994, 1655, 2414
TspDTI ATGAA 8 cut(s) 429, 1328, 1524, 1782, 2088, 2127, 2675, 2856
TspGWI ACGGA 2 cut(s) 44, 1539
TspMI CCCGGG 1 cut(s) 115
TspRI CASTG 3 cut(s) 254, 1003, 1888
Van91I CCANNNNNTGG 2 cut(s) 1228, 2197
Vha464I CTTAAG 1 cut(s) 304
VpaK11BI GGWCC 4 cut(s) 1258, 1619, 2187, 3028
XagI CCTNNNNNAGG 1 cut(s) 1292
XapI RAATTY 6 cut(s) 804, 1331, 1392, 1458, 2452, 2578
XbaI TCTAGA 1 cut(s) 2906
XceI RCATGY 6 cut(s) 136, 1415, 1876, 2422, 2526, 2764
XcmI CCANNNNNNNNNTGG 1 cut(s) 406
XmaI CCCGGG 1 cut(s) 115
XmaJI CCTAGG 1 cut(s) 2603
XmiI GTMKAC 2 cut(s) 1815, 1997
XmnI GAANNNNTTC 1 cut(s) 603
XspI CTAG 8 cut(s) 155, 1716, 1925, 1977, 2391, 2565, 2604, 2907
ZraI GACGTC 1 cut(s) 1234
ZrmI AGTACT 1 cut(s) 2563
Zsp2I ATGCAT 1 cut(s) 2947
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.