Rh6CG458200

Histidine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0009350
Physical Location & Seq
Reverse (-)
63335564 .. 63339698
4135 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG458200.1

Sequence Viewer

Length: 3060 bp
ATGGAGATCGATAATATTGAAGAAGTGGATATGGAGGAAGTCCTCCCTTCTATGTGGCCTGAGGATATAGATTCCGATAAGCAATTCAACATTGAAAAGCCAGGAAAAGATCAAGACATGTTGGAGGAGGTCACATTCAAAGAGAAACCAACTACAGTCGATTTCAGGCGGCTGTTGGAGCTAACCAAACACACAGATACAGGCTCTTCCCAATTAGCACACCTCGTAAAGCACTGGGAATATAAGCAGGCGAATGTGGTGAGACTTCTTAGAGAAGAACTTGATATCCTCAGCAAGCAAAAGCAGGAAGTTGAGCTCAAGAAATTGGAGATATTGGAAGAGCACCGCTTCGAGTCAGAGAGCTATGGCGGTGACAAACGCCCCATTTCCATTTTGGATGAAGTGTATCAGTTATGGGCTCCGGATGTTCCTCTAAAGAAGCTTGATGTTGTTGTGGAGAGTAAGAGAGTCGAGACAGAAGCCGAGTATGACAGTGTTGTGTACTGGAAGCAAAGGGCTATGCATTTGGAGAAGTTGTTGGAGGCAAGTGTTGCGAGAGAGCAGATTCTTGTCGATAAATTGCAGGAAAGCATTAAGAGTATTGAAAGACAGTCATCGCCGGTTGATGAGCTGTCTGAGATTTTGAAAAGAGCAGATAACTTCTTGCATTTTGTTCTTCAGAATGCCCCTGTTGTTATTGGTCATCAGGATAAAGAGTTGCGGTATCGCTTTATCTACAATCACTTCCCAAGTTTGGGAGAGCAGGACATTATAGGGAGAACAGATGTCGAGATTTTCAGTGGGGCTGGAGTTAAGGAATCTCAAGACTTCAAGAGAGAGGTTCTGGAAAAAGGGATACCGGCGAAAAGGGAGATTACATTCGAGACTGAATTGTTTGGGTCAAAGACGTTTTTGATATATGTGGAACCTGTTTTCAGCAAGGCAGGGGAGACAATTGGTATAAACTATATGGGGATGGAGGTGTCTGATCAGGTGAGGAAAAGAGAGAAGATGGCAAAGCTTCGTGAGGAAATAGCCGTCCAAAAGGCCAAGGAAACAGAGCTGAACAAGACAATTCACATAACGGAGGAGACAATGAGAGCAAAACAGATGCTGGCAACAATGTCTCATGAGATAAGGTCTCCTCTTTCTGGGGTTGTCAGCATGGCTGAGATTCTTACCACCACGAAACTTGACCGCGAGCAACGCCAGCTGTTGGAAGTCATGTTATCTTCAGGAGATTTGGTCCTCCAACTGATAAATGACATCCTTGACCTTTCCAAGGTTGAGTCAGGAGTAATGAAGTTGGAGGCTACAAAATTCCGGCCAAGACAGGTAGTTAGACATGTACTACAGACTGCGGCAGCATCATTGCAGAAAATTTTGACCTTGGAAGGACATGTTATGGAGAATGTTCCTGTGGAGATCATTGGAGATGTTTTAAGGATTCGTCAAATTCTAACAAACTTGATCAGTAATGCAATCAAGTTTACTCATGAAGGGAAAGTCGGGATAAAACTTTACATGGTAGCGGATCCAACTTCTGGAGATGATGAAAGATGTCTTCAGATGTCGAATGCAGTTCAATCAACGGTTTCAGTGAATGGATTGAATGAAGAGAAATCAGCATCACAAAATTGCTGCAATGGAGAAGGTATTCGTCGCAAAGAACCTTACCAAAATGGTTCTCTTCACGATGAACCTAGCACACCGGTTAAGAGTGAAGCCTCAATGAATGGAGATAAAGAGGAGGAACCCCATTCATCTGAAACAACAACAGTGTGGTTACGCTGCGATGTATATGACACAGGAATTGGAATACCAGAAAATGCTTTACCGACCCTTTTTAAAAGATACATGCAAGTGAGTGCTGATCATGCTCGAAAATATGGCGGGACAGGATTAGGACTAGCAATATGCAAACAATTGGTTGAACTAATGGGGGGCCAACTCACTGTGTCTAGCCGAGAACATTGTGGCTCCACATTCACATTTGTGTTACCTTACAAGGTTTCAACATCGTCGGAACATTCTGATGATCCTGATGAGCTCAGGGATATCGCTGAATATGACGATGCTGTAACTGATGATGAAGTAGCTGAAAGCTATTTCCAGTTCCAACCACGCACTTTAGGTTCTCTGTTCTCTTCTAATGGCACCGGCAGGACGCAAAAACTGTTACCACATAAACTCAATGGATTATCACAACATGCATACTCGTTTCCATATAATAACATCAGACCAGAGGAGATTACTTCAGTTGAAAATTGCTGTTCTGCAGTCGATGATGTTGCTGAGACATTATCTGAACCAGAGAGTTCAGTAAGTCACATGCCGAATTCCAATGGCGAAACTCCAAATTCCAATGGCGAAACTCCAGCATGTAGGGACAGAAAGGGTCAAGAGGACTCAAACAGTCAATCCCAAAATTCTTCAACAGATTCAAATCATCATGCAGAGGCAAGCAGAGAAAATGGTGTAGCAGCAAAGACAAGAGAACCCCAAGGAACGTGTAATAGACAGGAGAAATCAGATGCAGATTCAGAGTGTAGCTCTGGCAACAGTCCAAAAATACCAAAATCAACATCGAATCCTAAGATTCTTCTTGTTGAAGATAACAAGATCAATATTATGGTGGCACAGTCAATGATGAAGCAGTTAGGCCAAAGCTTAGAGGTTGTAAATGACGGAGCTGAAGCTGTGCGTGCAGTTCAACGCTGTAGTTACGATCTGATTCTGATGGATGTTCATATGCCAGTTATGGATGGGCTACAAGCTACACGAATAATACGTTCTTTCGAAGAAACTGGCAACTGGGAAGCTGCTAAGAAAGCTGGAATTGAGCAATCTGTGCCTTCTTCAAATACACTACAAAACGGCCACGGACATATGCCTTGTAGAAAGCGAATCCCAATCATTGCGATGACAGCAAATGCATTTGCAGAGAGCGCAGAAGAGTGTTTTGCAAATGGTATGGACTCGTTTGTGCCGAAACCTGTAACGCGCCAAAAATTGAAAGAGCGGCTTGAACAATATCTACCCGATTGCAACCTAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000302 GO:0002237 GO:0003674 GO:0003824 GO:0004672 GO:0004673 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009617 GO:0009636 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009755 GO:0009787 GO:0009788 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010104 GO:0010105 GO:0010119 GO:0010646 GO:0010648 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016775 GO:0018106 GO:0018193 GO:0018202 GO:0019538 GO:0022622 GO:0023051 GO:0023052 GO:0023057 GO:0032501 GO:0032502 GO:0032870 GO:0033554 GO:0034599 GO:0034614 GO:0035690 GO:0036211 GO:0042221 GO:0042493 GO:0042542 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046677 GO:0048364 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0070297 GO:0070298 GO:0070301 GO:0070887 GO:0071216 GO:0071219 GO:0071236 GO:0071241 GO:0071310 GO:0071368 GO:0071495 GO:0071704 GO:0071731 GO:0071732 GO:0071944 GO:0090333 GO:0097237 GO:0097366 GO:0099402 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902170 GO:1902531 GO:1902532 GO:1905957 GO:1905958
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HATPase_c HisKA Response_reg

Protein Analysis

1019

Amino Acids

114.45

Weight (kDa)

5.12

Isoelectric Point (pI)

53.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HisKA PF00512 369 - 432 1.7e-18 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 593 - 669 2.9e-20 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Response_reg PF00072 867 - 934 3.8e-13 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 2156
AccB7I CCANNNNNTGG 2 cut(s) 1216, 1544
AccBSI CCGCTC 1 cut(s) 3022
AccII CGCG 2 cut(s) 1200, 3004
AccIII TCCGGA 1 cut(s) 421
AciI CCGC 9 cut(s) 169, 346, 369, 721, 1198, 1361, 1532, 1893, 3022
AclWI GGATC 3 cut(s) 1529, 1542, 2033
AcoI YGGCCR 2 cut(s) 1325, 2878
AcsI RAATTY 6 cut(s) 1319, 1380, 1455, 2338, 2359, 2428
AcuI CTGAAG 5 cut(s) 662, 1218, 1550, 2241, 2715
AfaI GTAC 2 cut(s) 503, 1350
AfiI CCNNNNNNNGG 6 cut(s) 754, 755, 1151, 1216, 1282, 1544
AflIII ACRYGT 4 cut(s) 117, 1345, 1399, 2510
AgeI ACCGGT 1 cut(s) 1711
AjnI CCWGG 1 cut(s) 100
AleI CACNNNNGTG 1 cut(s) 1994
AloI GAACNNNNNNTCC 2 cut(s) 270, 302
Alw21I GWGCWC 3 cut(s) 318, 345, 2052
Alw26I GTCTC 8 cut(s) 256, 467, 878, 944, 1085, 1131, 1146, 2291
AlwI GGATC 3 cut(s) 1529, 1542, 2033
AlwNI CAGNNNCTG 1 cut(s) 1114
Aor13HI TCCGGA 1 cut(s) 421
AoxI GGCC 6 cut(s) 56, 1047, 1325, 1945, 2662, 2878
ApeKI GCWGC 5 cut(s) 1364, 1641, 1791, 2483, 2822
ApoI RAATTY 6 cut(s) 1319, 1380, 1455, 2338, 2359, 2428
AsiGI ACCGGT 1 cut(s) 1711
Asp700I GAANNNNTTC 1 cut(s) 1656
AspLEI GCGC 2 cut(s) 2951, 3006
AspS9I GGNCC 2 cut(s) 1246, 1945
AsuHPI GGTGA 3 cut(s) 271, 383, 1006
AsuII TTCGAA 1 cut(s) 2799
AvaII GGWCC 1 cut(s) 1246
AxyI CCTNAGG 1 cut(s) 60
BamHI GGATCC 1 cut(s) 1534
BanI GGYRCC 1 cut(s) 2156
BanII GRGCYC 3 cut(s) 318, 421, 2052
BbsI GAAGAC 1 cut(s) 1556
Bbv12I GWGCWC 3 cut(s) 318, 345, 2052
BbvCI CCTCAGC 1 cut(s) 290
BbvI GCAGC 5 cut(s) 1376, 1628, 1778, 2495, 2809
BccI CCATC 4 cut(s) 970, 1006, 2734, 2759
BceAI ACGGC 2 cut(s) 1022, 2893
BcgI CGANNNNNNTGC 2 cut(s) 2273, 2307
BciT130I CCWGG 1 cut(s) 102
BciVI GTATCC 1 cut(s) 849
BclI TGATCA 3 cut(s) 988, 1470, 1873
BcoDI GTCTC 8 cut(s) 256, 467, 878, 944, 1085, 1131, 1146, 2291
BfaI CTAG 4 cut(s) 1704, 1910, 1962, 3053
BfmI CTRYAG 4 cut(s) 153, 1352, 2277, 2719
BfuI GTATCC 1 cut(s) 849
BisI GCNGC 8 cut(s) 170, 1362, 1365, 1642, 1792, 2484, 2823, 3023
BlsI GCNGC 8 cut(s) 171, 1363, 1366, 1643, 1793, 2485, 2824, 3024
Bme1390I CCNGG 1 cut(s) 102
Bme18I GGWCC 1 cut(s) 1246
BmgT120I GGNCC 2 cut(s) 1246, 1945
BmiI GGNNCC 7 cut(s) 420, 927, 1536, 1755, 1946, 1981, 2158
BmrFI CCNGG 1 cut(s) 102
BmrI ACTGGG 2 cut(s) 244, 2824
BmsI GCATC 5 cut(s) 1101, 1376, 1637, 2065, 2524
BmuI ACTGGG 2 cut(s) 244, 2824
BpiI GAAGAC 1 cut(s) 1556
BplI GAGNNNNNCTC 2 cut(s) 2537, 2569
BpmI CTGGAG 3 cut(s) 828, 1566, 2361
Bpu10I CCTNAGC 2 cut(s) 290, 2051
Bpu14I TTCGAA 1 cut(s) 2799
BpuEI CTTGAG 2 cut(s) 302, 807
Bsa29I ATCGAT 1 cut(s) 9
BsaBI GATNNNNATC 1 cut(s) 2445
BsaI GGTCTC 1 cut(s) 1146
BsaJI CCNNGG 5 cut(s) 1050, 1281, 1389, 2503, 2881
BsaWI WCCGGW 2 cut(s) 421, 1711
BsaXI ACNNNNNCTCC 4 cut(s) 521, 551, 1230, 1260
Bsc4I CCNNNNNNNGG 6 cut(s) 754, 755, 1151, 1216, 1282, 1544
Bse118I RCCGGY 4 cut(s) 619, 859, 1711, 2159
Bse1I ACTGG 6 cut(s) 239, 509, 2113, 2756, 2812, 2819
Bse21I CCTNAGG 1 cut(s) 60
Bse3DI GCAATG 3 cut(s) 1370, 1651, 2916
Bse8I GATNNNNATC 1 cut(s) 2445
BseAI TCCGGA 1 cut(s) 421
BseBI CCWGG 1 cut(s) 102
BseCI ATCGAT 1 cut(s) 9
BseDI CCNNGG 5 cut(s) 1050, 1281, 1389, 2503, 2881
BseGI GGATG 6 cut(s) 403, 430, 981, 1266, 2749, 2770
BseJI GATNNNNATC 1 cut(s) 2445
BseLI CCNNNNNNNGG 6 cut(s) 754, 755, 1151, 1216, 1282, 1544
BseMI GCAATG 3 cut(s) 1370, 1651, 2916
BseMII CTCAG 6 cut(s) 51, 304, 627, 1161, 2065, 2286
BseNI ACTGG 6 cut(s) 239, 509, 2113, 2756, 2812, 2819
BseRI GAGGAG 5 cut(s) 140, 1103, 1134, 1763, 2261
BseXI GCAGC 5 cut(s) 1376, 1628, 1778, 2495, 2809
BsgI GTGCAG 1 cut(s) 2727
Bsh1236I CGCG 2 cut(s) 1200, 3004
BshFI GGCC 6 cut(s) 58, 1049, 1327, 1947, 2664, 2880
BshNI GGYRCC 1 cut(s) 2156
BshTI ACCGGT 1 cut(s) 1711
BshVI ATCGAT 1 cut(s) 9
BsiHKAI GWGCWC 3 cut(s) 318, 345, 2052
BsiSI CCGG 6 cut(s) 422, 620, 860, 1324, 1712, 2160
BslFI GGGAC 2 cut(s) 1909, 2402
BslI CCNNNNNNNGG 6 cut(s) 754, 755, 1151, 1216, 1282, 1544
BsmAI GTCTC 8 cut(s) 256, 467, 878, 944, 1085, 1131, 1146, 2291
BsmFI GGGAC 2 cut(s) 1909, 2402
BsmI GAATGC 2 cut(s) 688, 1582
BsnI GGCC 6 cut(s) 58, 1049, 1327, 1947, 2664, 2880
Bso31I GGTCTC 1 cut(s) 1146
Bsp119I TTCGAA 1 cut(s) 2799
Bsp1286I GDGCHC 4 cut(s) 318, 345, 421, 2052
Bsp13I TCCGGA 1 cut(s) 421
BspACI CCGC 9 cut(s) 169, 346, 369, 721, 1198, 1361, 1532, 1893, 3022
BspANI GGCC 6 cut(s) 58, 1049, 1327, 1947, 2664, 2880
BspCNI CTCAG 6 cut(s) 52, 303, 628, 1162, 2064, 2287
BspDI ATCGAT 1 cut(s) 9
BspEI TCCGGA 1 cut(s) 421
BspFNI CGCG 2 cut(s) 1200, 3004
BspHI TCATGA 2 cut(s) 1129, 1495
BspLI GGNNCC 7 cut(s) 420, 927, 1536, 1755, 1946, 1981, 2158
BspMAI CTGCAG 1 cut(s) 2281
BspPI GGATC 3 cut(s) 1529, 1542, 2033
BspQI GCTCTTC 2 cut(s) 211, 333
BspT104I TTCGAA 1 cut(s) 2799
BspT107I GGYRCC 1 cut(s) 2156
BspTNI GGTCTC 1 cut(s) 1146
BsrBI CCGCTC 1 cut(s) 3022
BsrDI GCAATG 3 cut(s) 1370, 1651, 2916
BsrFI RCCGGY 4 cut(s) 619, 859, 1711, 2159
BsrI ACTGG 6 cut(s) 239, 509, 2113, 2756, 2812, 2819
BssAI RCCGGY 4 cut(s) 619, 859, 1711, 2159
BssECI CCNNGG 5 cut(s) 1050, 1281, 1389, 2503, 2881
BssT1I CCWWGG 4 cut(s) 1050, 1281, 1389, 2503
Bst2UI CCWGG 1 cut(s) 102
Bst6I CTCTTC 6 cut(s) 211, 333, 1611, 1695, 2152, 2949
BstAPI GCANNNNNTGC 2 cut(s) 551, 2851
BstBI TTCGAA 1 cut(s) 2799
BstC8I GCNNGC 7 cut(s) 249, 296, 1116, 1202, 1211, 2464, 2706
BstDSI CCRYGG 1 cut(s) 2881
BstENI CCTNNNNNAGG 1 cut(s) 1280
BstF5I GGATG 6 cut(s) 403, 430, 981, 1266, 2749, 2770
BstFNI CGCG 2 cut(s) 1200, 3004
BstHHI GCGC 2 cut(s) 2951, 3006
BstMAI GTCTC 8 cut(s) 256, 467, 878, 944, 1085, 1131, 1146, 2291
BstNI CCWGG 1 cut(s) 102
BstNSI RCATGY 7 cut(s) 121, 1349, 1403, 1861, 2213, 2335, 2385
BstSCI CCNGG 1 cut(s) 100
BstSFI CTRYAG 4 cut(s) 153, 1352, 2277, 2719
BstUI CGCG 2 cut(s) 1200, 3004
BstV1I GCAGC 5 cut(s) 1376, 1628, 1778, 2495, 2809
BstV2I GAAGAC 1 cut(s) 1556
BstX2I RGATCY 1 cut(s) 1534
BstYI RGATCY 1 cut(s) 1534
Bsu15I ATCGAT 1 cut(s) 9
Bsu36I CCTNAGG 1 cut(s) 60
BsuI GTATCC 1 cut(s) 849
BsuRI GGCC 6 cut(s) 58, 1049, 1327, 1947, 2664, 2880
BsuTUI ATCGAT 1 cut(s) 9
BtgI CCRYGG 1 cut(s) 2881
BtgZI GCGATG 3 cut(s) 600, 1809, 2936
BtsCI GGATG 6 cut(s) 403, 430, 981, 1266, 2749, 2770
BtsIMutI CAGTG 6 cut(s) 232, 499, 805, 1605, 1785, 1953
Cac8I GCNNGC 7 cut(s) 249, 296, 1116, 1202, 1211, 2464, 2706
CaiI CAGNNNCTG 1 cut(s) 1114
CciI TCATGA 2 cut(s) 1129, 1495
CfoI GCGC 2 cut(s) 2951, 3006
Cfr10I RCCGGY 4 cut(s) 619, 859, 1711, 2159
Cfr13I GGNCC 2 cut(s) 1246, 1945
ClaI ATCGAT 1 cut(s) 9
CseI GACGC 1 cut(s) 2176
Csp6I GTAC 2 cut(s) 502, 1349
CspAI ACCGGT 1 cut(s) 1711
CspCI CAANNNNNGTGG 2 cut(s) 1174, 1209
CviQI GTAC 2 cut(s) 502, 1349
DraI TTTAAA 1 cut(s) 1849
EaeI YGGCCR 2 cut(s) 1325, 2878
Eam1104I CTCTTC 6 cut(s) 211, 333, 1611, 1695, 2152, 2949
EarI CTCTTC 6 cut(s) 211, 333, 1611, 1695, 2152, 2949
Ecl136II GAGCTC 2 cut(s) 316, 2050
Eco130I CCWWGG 4 cut(s) 1050, 1281, 1389, 2503
Eco24I GRGCYC 3 cut(s) 318, 421, 2052
Eco31I GGTCTC 1 cut(s) 1146
Eco32I GATATC 2 cut(s) 286, 2059
Eco47I GGWCC 1 cut(s) 1246
Eco53kI GAGCTC 2 cut(s) 316, 2050
Eco57I CTGAAG 5 cut(s) 662, 1218, 1550, 2241, 2715
Eco81I CCTNAGG 1 cut(s) 60
EcoICRI GAGCTC 2 cut(s) 316, 2050
EcoNI CCTNNNNNAGG 1 cut(s) 1280
EcoRI GAATTC 1 cut(s) 2338
EcoRII CCWGG 1 cut(s) 100
EcoRV GATATC 2 cut(s) 286, 2059
EcoT14I CCWWGG 4 cut(s) 1050, 1281, 1389, 2503
EcoT22I ATGCAT 3 cut(s) 525, 2215, 2938
EcoT38I GRGCYC 3 cut(s) 318, 421, 2052
ErhI CCWWGG 4 cut(s) 1050, 1281, 1389, 2503
FalI AAGNNNNNCTT 2 cut(s) 3009, 3041
FaqI GGGAC 2 cut(s) 1909, 2402
FauI CCCGC 1 cut(s) 1886
FauNDI CATATG 2 cut(s) 2751, 2889
FbaI TGATCA 3 cut(s) 988, 1470, 1873
Fnu4HI GCNGC 8 cut(s) 170, 1362, 1365, 1642, 1792, 2484, 2823, 3023
FokI GGATG 6 cut(s) 410, 437, 988, 1253, 2756, 2777
FriOI GRGCYC 3 cut(s) 318, 421, 2052
Fsp4HI GCNGC 8 cut(s) 170, 1362, 1365, 1642, 1792, 2484, 2823, 3023
FspBI CTAG 4 cut(s) 1704, 1910, 1962, 3053
GlaI GCGC 2 cut(s) 2950, 3005
GluI GCNGC 8 cut(s) 170, 1362, 1365, 1642, 1792, 2484, 2823, 3023
GsuI CTGGAG 3 cut(s) 828, 1566, 2361
HaeIII GGCC 6 cut(s) 58, 1049, 1327, 1947, 2664, 2880
HapII CCGG 6 cut(s) 422, 620, 860, 1324, 1712, 2160
HgaI GACGC 1 cut(s) 2176
HhaI GCGC 2 cut(s) 2951, 3006
Hin6I GCGC 2 cut(s) 2949, 3004
HinP1I GCGC 2 cut(s) 2949, 3004
HindIII AAGCTT 3 cut(s) 440, 1019, 2668
HpaII CCGG 6 cut(s) 422, 620, 860, 1324, 1712, 2160
HphI GGTGA 3 cut(s) 271, 383, 1006
Hpy166II GTNNAC 2 cut(s) 502, 1491
Hpy8I GTNNAC 2 cut(s) 502, 1491
Hpy99I CGWCG 2 cut(s) 1665, 2026
HpyAV CCTTC 5 cut(s) 57, 1388, 1493, 1646, 2865
HpyCH4IV ACGT 3 cut(s) 908, 2510, 2791
HpySE526I ACGT 3 cut(s) 908, 2510, 2791
HspAI GCGC 2 cut(s) 2949, 3004
Kpn2I TCCGGA 1 cut(s) 421
Ksp22I TGATCA 3 cut(s) 988, 1470, 1873
LguI GCTCTTC 2 cut(s) 211, 333
LmnI GCTCC 4 cut(s) 178, 424, 1985, 2690
Lsp1109I GCAGC 5 cut(s) 1376, 1628, 1778, 2495, 2809
LweI GCATC 5 cut(s) 1101, 1376, 1637, 2065, 2524
MaeI CTAG 4 cut(s) 1704, 1910, 1962, 3053
MaeII ACGT 3 cut(s) 908, 2510, 2791
MaeIII GTNAC 9 cut(s) 130, 371, 1785, 1998, 2080, 2178, 2327, 2723, 2998
MbiI CCGCTC 1 cut(s) 3022
MfeI CAATTG 2 cut(s) 954, 1925
MflI RGATCY 1 cut(s) 1534
MhlI GDGCHC 4 cut(s) 318, 345, 421, 2052
MlyI GAGTC 5 cut(s) 362, 477, 1298, 2402, 2972
MmeI TCCRAC 9 cut(s) 102, 156, 519, 1197, 1276, 1287, 1562, 2004, 2143
Mph1103I ATGCAT 3 cut(s) 525, 2215, 2938
MroI TCCGGA 1 cut(s) 421
MroXI GAANNNNTTC 1 cut(s) 1656
MseI TTAA 5 cut(s) 594, 813, 1442, 1716, 1848
MslI CAYNNNNRTG 4 cut(s) 1862, 1994, 2034, 2921
MspA1I CMGCKG 1 cut(s) 1213
MspI CCGG 6 cut(s) 422, 620, 860, 1324, 1712, 2160
MspR9I CCNGG 1 cut(s) 102
MunI CAATTG 2 cut(s) 954, 1925
Mva1269I GAATGC 2 cut(s) 688, 1582
MvaI CCWGG 1 cut(s) 102
MvnI CGCG 2 cut(s) 1200, 3004
NdeI CATATG 2 cut(s) 2751, 2889
NlaIV GGNNCC 7 cut(s) 420, 927, 1536, 1755, 1946, 1981, 2158
NmeAIII GCCGAG 2 cut(s) 508, 1991
NmuCI GTSAC 3 cut(s) 130, 371, 2327
NsiI ATGCAT 3 cut(s) 525, 2215, 2938
NspI RCATGY 7 cut(s) 121, 1349, 1403, 1861, 2213, 2335, 2385
NspV TTCGAA 1 cut(s) 2799
OliI CACNNNNGTG 1 cut(s) 1994
PagI TCATGA 2 cut(s) 1129, 1495
PciI ACATGT 3 cut(s) 117, 1345, 1399
PciSI GCTCTTC 2 cut(s) 211, 333
PcsI WCGNNNNNNNCGW 2 cut(s) 2788, 2987
PctI GAATGC 2 cut(s) 688, 1582
PdmI GAANNNNTTC 1 cut(s) 1656
PflMI CCANNNNNTGG 2 cut(s) 1216, 1544
PinAI ACCGGT 1 cut(s) 1711
PkrI GCNGC 8 cut(s) 171, 1363, 1366, 1643, 1793, 2485, 2824, 3024
PleI GAGTC 5 cut(s) 361, 476, 1297, 2402, 2972
PpsI GAGTC 5 cut(s) 361, 476, 1297, 2402, 2972
PscI ACATGT 3 cut(s) 117, 1345, 1399
Psp124BI GAGCTC 2 cut(s) 318, 2052
Psp6I CCWGG 1 cut(s) 100
PspGI CCWGG 1 cut(s) 100
PspN4I GGNNCC 7 cut(s) 420, 927, 1536, 1755, 1946, 1981, 2158
PspPI GGNCC 2 cut(s) 1246, 1945
PsrI GAACNNNNNNTAC 2 cut(s) 3021, 3053
PstI CTGCAG 1 cut(s) 2281
PstNI CAGNNNCTG 1 cut(s) 1114
PsuI RGATCY 1 cut(s) 1534
PvuII CAGCTG 1 cut(s) 1213
RsaI GTAC 2 cut(s) 503, 1350
RsaNI GTAC 2 cut(s) 502, 1349
RseI CAYNNNNRTG 4 cut(s) 1862, 1994, 2034, 2921
SacI GAGCTC 2 cut(s) 318, 2052
SapI GCTCTTC 2 cut(s) 211, 333
SaqAI TTAA 5 cut(s) 594, 813, 1442, 1716, 1848
SatI GCNGC 8 cut(s) 170, 1362, 1365, 1642, 1792, 2484, 2823, 3023
Sau96I GGNCC 2 cut(s) 1246, 1945
SchI GAGTC 5 cut(s) 362, 477, 1298, 2402, 2972
ScrFI CCNGG 1 cut(s) 102
SduI GDGCHC 4 cut(s) 318, 345, 421, 2052
SfaNI GCATC 5 cut(s) 1101, 1376, 1637, 2065, 2524
SfcI CTRYAG 4 cut(s) 153, 1352, 2277, 2719
SfuI TTCGAA 1 cut(s) 2799
SinI GGWCC 1 cut(s) 1246
SmiMI CAYNNNNRTG 4 cut(s) 1862, 1994, 2034, 2921
SmlI CTYRAG 2 cut(s) 317, 822
SmoI CTYRAG 2 cut(s) 317, 822
SsiI CCGC 9 cut(s) 169, 346, 369, 721, 1198, 1361, 1532, 1893, 3022
SspI AATATT 2 cut(s) 16, 2629
SspMI CTAG 4 cut(s) 1704, 1910, 1962, 3053
SstI GAGCTC 2 cut(s) 318, 2052
StyD4I CCNGG 1 cut(s) 100
StyI CCWWGG 4 cut(s) 1050, 1281, 1389, 2503
TaiI ACGT 3 cut(s) 911, 2513, 2794
TatI WGTACW 2 cut(s) 501, 1348
TauI GCSGC 3 cut(s) 172, 1364, 3025
Tru1I TTAA 5 cut(s) 594, 813, 1442, 1716, 1848
Tru9I TTAA 5 cut(s) 594, 813, 1442, 1716, 1848
TscAI CASTG 6 cut(s) 239, 499, 805, 1605, 1785, 1960
TseFI GTSAC 3 cut(s) 130, 371, 2327
TseI GCWGC 5 cut(s) 1364, 1641, 1791, 2483, 2822
Tsp45I GTSAC 3 cut(s) 130, 371, 2327
TspGWI ACGGA 3 cut(s) 1100, 2703, 2898
TspRI CASTG 6 cut(s) 239, 499, 805, 1605, 1785, 1960
Van91I CCANNNNNTGG 2 cut(s) 1216, 1544
VpaK11BI GGWCC 1 cut(s) 1246
XagI CCTNNNNNAGG 1 cut(s) 1280
XapI RAATTY 6 cut(s) 1319, 1380, 1455, 2338, 2359, 2428
XceI RCATGY 7 cut(s) 121, 1349, 1403, 1861, 2213, 2335, 2385
XcmI CCANNNNNNNNNTGG 1 cut(s) 391
XmnI GAANNNNTTC 1 cut(s) 1656
XspI CTAG 4 cut(s) 1704, 1910, 1962, 3053
Zsp2I ATGCAT 3 cut(s) 525, 2215, 2938
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.