MD08G1047400.v1.1

Histidine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0009350
Physical Location & Seq
Forward (+)
3585752 .. 3590654
4903 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1047400.v1.1.491

Sequence Viewer

Length: 3075 bp
ATGGTTTGGGATATGGAGACCGACAACATCGACGAAATGGACATGGAAGGTCTCCCTTCCATGTGGCCTGATGACATCGGAGCTGATGCCGGAAAGCAATTCAACATAGACAAGCCGGGGCAAGATCAGGACATGTTGGAGGAGGTGACATTCATAGAGGAACCAACAATCGTTGATTTCCAGCGCCTTGTCGAGCTGACAAACTACACCGATAAGGGCTCTTCGCAACTTGCATTCCTCGTAAAACACTGGGAGTATAAGCAGGCAAATGCCGTGCGCCTTCTCAGAGAAGAACTCGACATCTTAAGCAAGCAAAGGCAGGAAGTTGAGCTCAAGAAGTTGGAGATACTGGAAGAGCATCGGTTCGAGGCAGAGAGTTATGGGGGCGACAAGCGCCCCATTTCGATTTTGGATGAAGTTTATGACATATGGCCGGAAATTGCCCCGAAGAAACATGATGTTGTTGTGCTGACCAAGAGGGTTGAGGTAGAAGCCGAGTATGATACTGTTGCGTACTGGAAGCAGAGGGCAATGCAGTTGGAGAAACTGTTGGAGGCGAGTGCCGAGAGAGAGCAGATTCTTGTGGAAAAATTGCAGGAAAGCATTCAGAGTCTCGAAAGGCAGTCATCGCCAGTTGAAGAACTTTCTCAGATTTTGAAAAGAGCAGATAATTTCTTGCATTTTGTTCTTCAGAATGCACCTGTTGTTATTGGCCACCAGGATAAAGAATTGCGGTATCGATTTATCTATAATCACTTCCCAAGTTTGCAAGAGGAGGACATTATAGGAAGAACAGATGTTGAAATTTTTTCTGGAGCCGGCGTTAAAGAATCTCAAGATTTCAAGAAAGAGGTCCTAGAGAAAGGCATACCTGCAAAAAGGGAGATTACATTTGAGACGCCCTTGTTCGGGTCGAAAACGTTTTTGATATATGTGGAACCTGTCTTCAGCAAGGCAGGGGAGACAATTGGTATAAACTATATGGGGATGGATGTAACTGATCAGGTGAGGAAAAGAGAGAAGATGGCAAAGCTTCGTGAGGAAATCGCGGTCCAGAAAGCCAAGGAAACAGAACTGAACAAAACAATTCACATCACAGAGGAGACAATGCGAGCGAAACAGATGCTAGCAACAATGTCTCACGAGATAAGATCGCCTCTTTCCGGGGTTGTTAGCATGGCTGAGATTCTCACCACCACGAAACTGGATCACGAGCAACGCCAACTTTTGGACGTCATGTTATCTTCGGGAGATTTGGTCCTTCAACTCATAAACGACATCCTTGACCTTTCCAAGGTGGAGTCAGGAGTAATGAAGTTGGAAGCTACGAAATTCCGGCCACGAGAGGTACTAAGGCATGTACTACAGACTGCGGCGGCATCATTGCAGAAGATTTTGAACCTGGAAGGGCATGTATCTGAGGATGTTCCCATGGAAGTCATCGGCGATGTTTTAAGGATTCGTCAAATTCTAACCAATTTGGTCAGTAATGCGATCAAGTTCACTCATGAAGGGAAAGTTGGGATAAAACTTTACGTGGTATCGGATCCGTCATTGGAAGAGGGCGAAAGATGTCCCCAACATTCGGAAACAGTTCACTCGACGGTTTCAGAGAATGGACCGAAAGCGGAGAAACCAGCATCTCAAACTTCTTGTGATCAAGAAGGTTTCGATGGAGACCGACCGTACCAAAATCGCTCGCTTAACTACGAACCTAGAACCCCGGCCAAGAGTGAAGTTTCGATGGATGCAGCAGATCAAGAGGACGAACCTCGTTTGCCAGAAACGAAAACGGTTTGGTTACGATGCGATGTATACGACACCGGAATCGGAATACCAGAAAATGCTTTACCTTCTCTGTTTAAAAGATACATGCAAGTCAGTGCCGATCATGCTCAGAAATACGGAGGGACAGGATTAGGTCTAGCAATATGCAAACAATTGGTCGAGCTAATGGGGGGTCAACTCACAGTATCTAGCAAAGAAAAACGTGGGTCGACGTTTACATTTGTGCTCCCGTACAAGGTCTCGACATCGTCGGAACAACAATCTGATGACCCTGATGATTCCGACGAAGCTTCGGATATGGCTAGTCATGATGGCGCAACCGATGAAGTAGCTGAGAGCTATTTCCAGTTCCAGCCACGGACTTTGGGTTCTCTGTTCTCTTCTAATGGATCCGCCAGGACACCAAAATTGTTAACGCATAAGATGGGGTTTACTGGTTCGCATAAACTCAATGGGCTTGCACAAAATTCATACTCGTTTCCGAACGGTAACATGAGACCGGAGGAGATAGCTTCGGTTGAAAACTCTTGCTCGGAAGGTGATTTTGCGGAGACGTTACCAGAACCAGAGAGATCCTCGGCGAGTCACAACCGAGACCCTAGTAGTGAAAATCCAGCTAGTACAAACAAACAGGGCCGAGATGACATGGATAACCGATTCCAAGACTCTATAAGCACAGATTCAAGTCATCCTTCAGCGGAAGGAAGAGAAATAGCTGTAGAAGCAACCACAAGAGAACCCCAAGGTACATGTAAGAGGCAGGAGAAACCCGACGCAGACTCGGACAGTACTTGTAGCAGCAGTTCAAAAATACCAAAACCAACATCAAAACCTAAAATTCTTCTTGTTGAAGATAACAAGATTAACGTTATGGTGGCACAGAACATGATGAAGCGGCTAGGCCATAGCATGGATGTTGTGAACAACGGAATGGAAGCTGTGTGTGCAGTTCAACGCGGTAGATACGATCTCATCCTCATGGATGTTCACATGCCAGTTATGGATGGCCTTCAAACTACAAGAATTATACGTTCTTACGAAGAAACTGGCAACTGGGATGCAGCTGAGAAAGCTGGAATTGAGCTTCCTCTGCCTTCATCAGAAACAACACAGAACGGTCATGGCTACACGCCTTGTGGAAAGCGAATCCCGATCATAGCGATGACAGCAAATGCATTTTCAGAGAGTGCAGAAGAATGTTTCAGATGTGGAATGGACTCATTCGTGCCAAAGCCCGTAACATTTCAAAAATTGAAAGACCGTCTCGAACAATACCTACACGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000302 GO:0002237 GO:0003674 GO:0003824 GO:0004672 GO:0004673 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009617 GO:0009636 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009755 GO:0009787 GO:0009788 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010104 GO:0010105 GO:0010119 GO:0010646 GO:0010648 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016775 GO:0018106 GO:0018193 GO:0018202 GO:0019538 GO:0022622 GO:0023051 GO:0023052 GO:0023057 GO:0032501 GO:0032502 GO:0032870 GO:0033554 GO:0034599 GO:0034614 GO:0035690 GO:0036211 GO:0042221 GO:0042493 GO:0042542 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046677 GO:0048364 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0070297 GO:0070298 GO:0070301 GO:0070887 GO:0071216 GO:0071219 GO:0071236 GO:0071241 GO:0071310 GO:0071368 GO:0071495 GO:0071704 GO:0071731 GO:0071732 GO:0071944 GO:0090333 GO:0097237 GO:0097366 GO:0099402 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902170 GO:1902531 GO:1902532 GO:1905957 GO:1905958
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HATPase_c HisKA Response_reg

Protein Analysis

1025

Amino Acids

115.09

Weight (kDa)

4.96

Isoelectric Point (pI)

49.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HisKA PF00512 373 - 436 6.8e-19 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 598 - 674 6.7e-21 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Response_reg PF00072 876 - 943 5.4e-13 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1236
Acc36I ACCTGC 1 cut(s) 880
AccB7I CCANNNNNTGG 2 cut(s) 1228, 2698
AccI GTMKAC 2 cut(s) 1815, 1997
AccII CGCG 2 cut(s) 1049, 2745
AclI AACGTT 2 cut(s) 920, 2655
AclWI GGATC 6 cut(s) 1215, 1541, 1554, 2172, 2185, 2355
AcoI YGGCCR 4 cut(s) 431, 712, 1337, 1725
AcsI RAATTY 5 cut(s) 804, 1331, 1467, 2254, 2625
AcuI CTGAAG 3 cut(s) 674, 931, 2466
AcyI GRCGYC 2 cut(s) 899, 1233
AfaI GTAC 8 cut(s) 515, 1350, 1362, 1688, 2021, 2410, 2536, 2578
AfiI CCNNNNNNNGG 8 cut(s) 908, 909, 1163, 1228, 1294, 1585, 2023, 2698
AflII CTTAAG 1 cut(s) 304
AflIII ACRYGT 2 cut(s) 132, 2537
AjnI CCWGG 3 cut(s) 717, 1401, 2183
AjuI GAANNNNNNNTTGG 2 cut(s) 1503, 1535
AloI GAACNNNNNNTCC 2 cut(s) 2140, 2172
Alw21I GWGCWC 2 cut(s) 333, 2016
AlwI GGATC 6 cut(s) 1215, 1541, 1554, 2172, 2185, 2355
AoxI GGCC 8 cut(s) 65, 431, 712, 1337, 1725, 2422, 2689, 2794
ApeKI GCWGC 3 cut(s) 1751, 2586, 2849
ApoI RAATTY 5 cut(s) 804, 1331, 1467, 2254, 2625
ArsI GACNNNNNNTTYG 2 cut(s) 1929, 1961
Asp700I GAANNNNTTC 2 cut(s) 603, 1593
AspLEI GCGC 4 cut(s) 186, 279, 396, 2105
AspS9I GGNCC 5 cut(s) 853, 1051, 1258, 1619, 2422
AsuC2I CCSGG 3 cut(s) 117, 1165, 1724
AsuHPI GGTGA 4 cut(s) 157, 1018, 1183, 2339
AsuNHI GCTAGC 1 cut(s) 1126
AvaII GGWCC 4 cut(s) 853, 1051, 1258, 1619
BalI TGGCCA 1 cut(s) 714
BamHI GGATCC 2 cut(s) 1546, 2177
BanII GRGCYC 2 cut(s) 221, 333
BauI CACGAG 3 cut(s) 1142, 1211, 1341
BbsI GAAGAC 1 cut(s) 937
Bbv12I GWGCWC 2 cut(s) 333, 2016
BbvI GCAGC 3 cut(s) 1763, 2598, 2861
BccI CCATC 7 cut(s) 982, 1018, 1667, 1738, 2093, 2206, 2786
BceAI ACGGC 1 cut(s) 257
BcgI CGANNNNNNTGC 2 cut(s) 1105, 1139
BciT130I CCWGG 3 cut(s) 719, 1403, 2185
BclI TGATCA 2 cut(s) 1000, 1657
BcnI CCSGG 3 cut(s) 117, 1165, 1724
BfaI CTAG 9 cut(s) 857, 1127, 1716, 1925, 1977, 2091, 2388, 2406, 2687
BfmI CTRYAG 2 cut(s) 1364, 2505
BfoI RGCGCY 2 cut(s) 187, 397
BfrI CTTAAG 1 cut(s) 304
BfuAI ACCTGC 1 cut(s) 880
BisI GCNGC 6 cut(s) 1374, 1377, 1752, 2587, 2684, 2850
BlsI GCNGC 6 cut(s) 1375, 1378, 1753, 2588, 2685, 2851
BmcAI AGTACT 1 cut(s) 2578
Bme1390I CCNGG 6 cut(s) 117, 719, 1165, 1403, 1724, 2185
Bme18I GGWCC 4 cut(s) 853, 1051, 1258, 1619
BmgT120I GGNCC 5 cut(s) 853, 1051, 1258, 1619, 2422
BmiI GGNNCC 5 cut(s) 162, 817, 939, 1548, 2179
BmrFI CCNGG 6 cut(s) 117, 719, 1165, 1403, 1724, 2185
BmrI ACTGGG 2 cut(s) 259, 2851
BmsI GCATC 8 cut(s) 76, 367, 1113, 1388, 1649, 1738, 1796, 2836
BmtI GCTAGC 1 cut(s) 1130
BmuI ACTGGG 2 cut(s) 259, 2851
BpiI GAAGAC 1 cut(s) 937
BplI GAGNNNNNCTC 4 cut(s) 279, 311, 2348, 2380
BpmI CTGGAG 1 cut(s) 834
BpuEI CTTGAG 2 cut(s) 317, 819
BpuMI CCSGG 3 cut(s) 117, 1165, 1724
Bsa29I ATCGAT 1 cut(s) 739
BsaAI YACGTR 1 cut(s) 1537
BsaHI GRCGYC 2 cut(s) 899, 1233
BsaI GGTCTC 6 cut(s) 11, 56, 1671, 2032, 2278, 2376
BsaJI CCNNGG 9 cut(s) 116, 1062, 1164, 1293, 1431, 1722, 2144, 2364, 2530
BsaWI WCCGGW 2 cut(s) 1823, 2287
BsaXI ACNNNNNCTCC 6 cut(s) 533, 563, 807, 837, 1242, 1272
Bsc4I CCNNNNNNNGG 8 cut(s) 908, 909, 1163, 1228, 1294, 1585, 2023, 2698
Bse118I RCCGGY 1 cut(s) 818
Bse3DI GCAATG 2 cut(s) 537, 1382
BseBI CCWGG 3 cut(s) 719, 1403, 2185
BseCI ATCGAT 1 cut(s) 739
BseDI CCNNGG 9 cut(s) 116, 1062, 1164, 1293, 1431, 1722, 2144, 2364, 2530
BseLI CCNNNNNNNGG 8 cut(s) 908, 909, 1163, 1228, 1294, 1585, 2023, 2698
BseMI GCAATG 2 cut(s) 537, 1382
BseMII CTCAG 7 cut(s) 298, 662, 1173, 1410, 1910, 2112, 2844
BseRI GAGGAG 4 cut(s) 155, 788, 1115, 2306
BseXI GCAGC 3 cut(s) 1763, 2598, 2861
BsgI GTGCAG 2 cut(s) 2754, 2997
Bsh1236I CGCG 2 cut(s) 1049, 2745
Bsh1285I CGRYCG 1 cut(s) 1685
BshFI GGCC 8 cut(s) 67, 433, 714, 1339, 1727, 2424, 2691, 2796
BshVI ATCGAT 1 cut(s) 739
BsiEI CGRYCG 1 cut(s) 1685
BsiHKAI GWGCWC 2 cut(s) 333, 2016
BsiSI CCGG 9 cut(s) 90, 116, 434, 819, 1164, 1336, 1724, 1824, 2288
BslFI GGGAC 2 cut(s) 1560, 1924
BslI CCNNNNNNNGG 8 cut(s) 908, 909, 1163, 1228, 1294, 1585, 2023, 2698
BsmBI CGTCTC 3 cut(s) 890, 2333, 3056
BsmFI GGGAC 2 cut(s) 1560, 1924
BsmI GAATGC 3 cut(s) 233, 603, 700
BsnI GGCC 8 cut(s) 67, 433, 714, 1339, 1727, 2424, 2691, 2796
Bso31I GGTCTC 6 cut(s) 11, 56, 1671, 2032, 2278, 2376
Bsp1286I GDGCHC 3 cut(s) 221, 333, 2016
Bsp19I CCATGG 1 cut(s) 1431
BspANI GGCC 8 cut(s) 67, 433, 714, 1339, 1727, 2424, 2691, 2796
BspCNI CTCAG 7 cut(s) 297, 661, 1174, 1411, 1909, 2113, 2845
BspDI ATCGAT 1 cut(s) 739
BspFNI CGCG 2 cut(s) 1049, 2745
BspHI TCATGA 2 cut(s) 1507, 2095
BspLI GGNNCC 5 cut(s) 162, 817, 939, 1548, 2179
BspMI ACCTGC 1 cut(s) 880
BspOI GCTAGC 1 cut(s) 1130
BspPI GGATC 6 cut(s) 1215, 1541, 1554, 2172, 2185, 2355
BspQI GCTCTTC 2 cut(s) 226, 348
BspTI CTTAAG 1 cut(s) 304
BspTNI GGTCTC 6 cut(s) 11, 56, 1671, 2032, 2278, 2376
BsrDI GCAATG 2 cut(s) 537, 1382
BsrFI RCCGGY 1 cut(s) 818
BssAI RCCGGY 1 cut(s) 818
BssECI CCNNGG 9 cut(s) 116, 1062, 1164, 1293, 1431, 1722, 2144, 2364, 2530
BssNAI GTATAC 1 cut(s) 1816
BssNI GRCGYC 2 cut(s) 899, 1233
BssSI CACGAG 3 cut(s) 1142, 1211, 1341
BssT1I CCWWGG 4 cut(s) 1062, 1293, 1431, 2530
Bst1107I GTATAC 1 cut(s) 1816
Bst2BI CACGAG 3 cut(s) 1142, 1211, 1341
Bst2UI CCWGG 3 cut(s) 719, 1403, 2185
Bst6I CTCTTC 5 cut(s) 226, 348, 1554, 2173, 2488
BstACI GRCGYC 2 cut(s) 899, 1233
BstAFI CTTAAG 1 cut(s) 304
BstBAI YACGTR 1 cut(s) 1537
BstC8I GCNNGC 7 cut(s) 264, 311, 820, 1113, 1128, 1700, 2247
BstDEI CTNAG 8 cut(s) 284, 648, 1182, 1352, 1419, 1896, 2121, 2853
BstDSI CCRYGG 2 cut(s) 1431, 2144
BstENI CCTNNNNNAGG 1 cut(s) 1292
BstFNI CGCG 2 cut(s) 1049, 2745
BstH2I RGCGCY 2 cut(s) 187, 397
BstHHI GCGC 4 cut(s) 186, 279, 396, 2105
BstMCI CGRYCG 1 cut(s) 1685
BstMWI GCNNNNNNNGC 8 cut(s) 393, 628, 1892, 2510, 2732, 2858, 2878, 2954
BstNI CCWGG 3 cut(s) 719, 1403, 2185
BstNSI RCATGY 6 cut(s) 136, 1361, 1415, 1876, 2541, 2782
BstSCI CCNGG 6 cut(s) 115, 717, 1163, 1401, 1722, 2183
BstSFI CTRYAG 2 cut(s) 1364, 2505
BstUI CGCG 2 cut(s) 1049, 2745
BstV1I GCAGC 3 cut(s) 1763, 2598, 2861
BstV2I GAAGAC 1 cut(s) 937
BstX2I RGATCY 3 cut(s) 1546, 2177, 2360
BstXI CCANNNNNNTGG 1 cut(s) 1204
BstYI RGATCY 3 cut(s) 1546, 2177, 2360
BstZ17I GTATAC 1 cut(s) 1816
Bsu15I ATCGAT 1 cut(s) 739
BsuRI GGCC 8 cut(s) 67, 433, 714, 1339, 1727, 2424, 2691, 2796
BsuTUI ATCGAT 1 cut(s) 739
BtgI CCRYGG 2 cut(s) 1431, 2144
BtgZI GCGATG 4 cut(s) 612, 1461, 1824, 2963
BtsIMutI CAGTG 2 cut(s) 247, 1888
BveI ACCTGC 1 cut(s) 880
Cac8I GCNNGC 7 cut(s) 264, 311, 820, 1113, 1128, 1700, 2247
CciI TCATGA 2 cut(s) 1507, 2095
CfoI GCGC 4 cut(s) 186, 279, 396, 2105
Cfr10I RCCGGY 1 cut(s) 818
Cfr13I GGNCC 5 cut(s) 853, 1051, 1258, 1619, 2422
ClaI ATCGAT 1 cut(s) 739
CseI GACGC 2 cut(s) 907, 2570
Csp6I GTAC 8 cut(s) 514, 1349, 1361, 1687, 2020, 2409, 2535, 2577
CspCI CAANNNNNGTGG 2 cut(s) 2133, 2168
CviQI GTAC 8 cut(s) 514, 1349, 1361, 1687, 2020, 2409, 2535, 2577
DdeI CTNAG 8 cut(s) 284, 648, 1182, 1352, 1419, 1896, 2121, 2853
DraI TTTAAA 1 cut(s) 1864
EaeI YGGCCR 4 cut(s) 431, 712, 1337, 1725
Eam1104I CTCTTC 5 cut(s) 226, 348, 1554, 2173, 2488
EarI CTCTTC 5 cut(s) 226, 348, 1554, 2173, 2488
EciI GGCGGA 1 cut(s) 2170
Ecl136II GAGCTC 1 cut(s) 331
Eco130I CCWWGG 4 cut(s) 1062, 1293, 1431, 2530
Eco24I GRGCYC 2 cut(s) 221, 333
Eco31I GGTCTC 6 cut(s) 11, 56, 1671, 2032, 2278, 2376
Eco47I GGWCC 4 cut(s) 853, 1051, 1258, 1619
Eco53kI GAGCTC 1 cut(s) 331
Eco57I CTGAAG 3 cut(s) 674, 931, 2466
EcoICRI GAGCTC 1 cut(s) 331
EcoNI CCTNNNNNAGG 1 cut(s) 1292
EcoO109I RGGNCCY 1 cut(s) 853
EcoRII CCWGG 3 cut(s) 717, 1401, 2183
EcoT14I CCWWGG 4 cut(s) 1062, 1293, 1431, 2530
EcoT22I ATGCAT 1 cut(s) 2965
EcoT38I GRGCYC 2 cut(s) 221, 333
ErhI CCWWGG 4 cut(s) 1062, 1293, 1431, 2530
Esp3I CGTCTC 3 cut(s) 890, 2333, 3056
FalI AAGNNNNNCTT 2 cut(s) 2464, 2496
FaqI GGGAC 2 cut(s) 1560, 1924
FauNDI CATATG 1 cut(s) 428
FbaI TGATCA 2 cut(s) 1000, 1657
FblI GTMKAC 2 cut(s) 1815, 1997
Fnu4HI GCNGC 6 cut(s) 1374, 1377, 1752, 2587, 2684, 2850
FriOI GRGCYC 2 cut(s) 221, 333
Fsp4HI GCNGC 6 cut(s) 1374, 1377, 1752, 2587, 2684, 2850
FspBI CTAG 9 cut(s) 857, 1127, 1716, 1925, 1977, 2091, 2388, 2406, 2687
GlaI GCGC 4 cut(s) 185, 278, 395, 2104
GluI GCNGC 6 cut(s) 1374, 1377, 1752, 2587, 2684, 2850
GsuI CTGGAG 1 cut(s) 834
HaeII RGCGCY 2 cut(s) 187, 397
HaeIII GGCC 8 cut(s) 67, 433, 714, 1339, 1727, 2424, 2691, 2796
HapII CCGG 9 cut(s) 90, 116, 434, 819, 1164, 1336, 1724, 1824, 2288
HgaI GACGC 2 cut(s) 907, 2570
HhaI GCGC 4 cut(s) 186, 279, 396, 2105
Hin1I GRCGYC 2 cut(s) 899, 1233
Hin6I GCGC 4 cut(s) 184, 277, 394, 2103
HinP1I GCGC 4 cut(s) 184, 277, 394, 2103
HincII GTYRAC 3 cut(s) 1964, 1998, 2202
HindII GTYRAC 3 cut(s) 1964, 1998, 2202
HindIII AAGCTT 2 cut(s) 1031, 2076
HpaI GTTAAC 1 cut(s) 2202
HpaII CCGG 9 cut(s) 90, 116, 434, 819, 1164, 1336, 1724, 1824, 2288
HphI GGTGA 4 cut(s) 157, 1018, 1183, 2339
Hpy99I CGWCG 6 cut(s) 35, 1606, 2002, 2041, 2075, 2564
HpyCH4IV ACGT 8 cut(s) 920, 1233, 1536, 1990, 2000, 2342, 2655, 2818
HpyF10VI GCNNNNNNNGC 8 cut(s) 393, 628, 1892, 2510, 2732, 2858, 2878, 2954
HpyF3I CTNAG 8 cut(s) 284, 648, 1182, 1352, 1419, 1896, 2121, 2853
HpySE526I ACGT 8 cut(s) 920, 1233, 1536, 1990, 2000, 2342, 2655, 2818
Hsp92I GRCGYC 2 cut(s) 899, 1233
HspAI GCGC 4 cut(s) 184, 277, 394, 2103
KroI GCCGGC 1 cut(s) 818
KroNI GCCGGC 1 cut(s) 820
Ksp22I TGATCA 2 cut(s) 1000, 1657
KspAI GTTAAC 1 cut(s) 2202
LguI GCTCTTC 2 cut(s) 226, 348
LmnI GCTCC 3 cut(s) 80, 815, 2019
Lsp1109I GCAGC 3 cut(s) 1763, 2598, 2861
LweI GCATC 8 cut(s) 76, 367, 1113, 1388, 1649, 1738, 1796, 2836
MaeI CTAG 9 cut(s) 857, 1127, 1716, 1925, 1977, 2091, 2388, 2406, 2687
MaeII ACGT 8 cut(s) 920, 1233, 1536, 1990, 2000, 2342, 2655, 2818
MaeIII GTNAC 7 cut(s) 145, 994, 1800, 2276, 2343, 2372, 3025
MfeI CAATTG 2 cut(s) 966, 1940
MflI RGATCY 3 cut(s) 1546, 2177, 2360
MhlI GDGCHC 3 cut(s) 221, 333, 2016
MlsI TGGCCA 1 cut(s) 714
MluNI TGGCCA 1 cut(s) 714
MlyI GAGTC 6 cut(s) 619, 1310, 2380, 2447, 2561, 2999
MmeI TCCRAC 7 cut(s) 117, 321, 519, 531, 1299, 2019, 2094
Mox20I TGGCCA 1 cut(s) 714
Mph1103I ATGCAT 1 cut(s) 2965
MroNI GCCGGC 1 cut(s) 818
MroXI GAANNNNTTC 2 cut(s) 603, 1593
MscI TGGCCA 1 cut(s) 714
MseI TTAA 7 cut(s) 305, 825, 1454, 1704, 1863, 2201, 2652
MslI CAYNNNNRTG 2 cut(s) 2765, 2948
Msp20I TGGCCA 1 cut(s) 714
MspA1I CMGCKG 2 cut(s) 2486, 2852
MspCI CTTAAG 1 cut(s) 304
MspI CCGG 9 cut(s) 90, 116, 434, 819, 1164, 1336, 1724, 1824, 2288
MspR9I CCNGG 6 cut(s) 117, 719, 1165, 1403, 1724, 2185
MunI CAATTG 2 cut(s) 966, 1940
Mva1269I GAATGC 3 cut(s) 233, 603, 700
MvaI CCWGG 3 cut(s) 719, 1403, 2185
MvnI CGCG 2 cut(s) 1049, 2745
MwoI GCNNNNNNNGC 8 cut(s) 393, 628, 1892, 2510, 2732, 2858, 2878, 2954
NaeI GCCGGC 1 cut(s) 820
NciI CCSGG 3 cut(s) 117, 1165, 1724
NcoI CCATGG 1 cut(s) 1431
NdeI CATATG 1 cut(s) 428
NgoMIV GCCGGC 1 cut(s) 818
NheI GCTAGC 1 cut(s) 1126
NlaIV GGNNCC 5 cut(s) 162, 817, 939, 1548, 2179
NmeAIII GCCGAG 4 cut(s) 520, 589, 2345, 2450
NmuCI GTSAC 2 cut(s) 145, 2372
NsiI ATGCAT 1 cut(s) 2965
NspI RCATGY 6 cut(s) 136, 1361, 1415, 1876, 2541, 2782
PagI TCATGA 2 cut(s) 1507, 2095
PciI ACATGT 2 cut(s) 132, 2537
PciSI GCTCTTC 2 cut(s) 226, 348
PctI GAATGC 3 cut(s) 233, 603, 700
PdiI GCCGGC 1 cut(s) 820
PdmI GAANNNNTTC 2 cut(s) 603, 1593
PfeI GAWTC 9 cut(s) 577, 830, 1186, 1459, 1827, 2066, 2445, 2468, 2934
PflFI GACNNNGTC 1 cut(s) 2035
PflMI CCANNNNNTGG 2 cut(s) 1228, 2698
PkrI GCNGC 6 cut(s) 1375, 1378, 1753, 2588, 2685, 2851
PleI GAGTC 6 cut(s) 618, 1309, 2379, 2447, 2561, 2999
PpsI GAGTC 6 cut(s) 618, 1309, 2379, 2447, 2561, 2999
Ppu21I YACGTR 1 cut(s) 1537
PpuMI RGGWCCY 1 cut(s) 853
PscI ACATGT 2 cut(s) 132, 2537
Psp124BI GAGCTC 1 cut(s) 333
Psp1406I AACGTT 2 cut(s) 920, 2655
Psp5II RGGWCCY 1 cut(s) 853
Psp6I CCWGG 3 cut(s) 717, 1401, 2183
PspGI CCWGG 3 cut(s) 717, 1401, 2183
PspN4I GGNNCC 5 cut(s) 162, 817, 939, 1548, 2179
PspPI GGNCC 5 cut(s) 853, 1051, 1258, 1619, 2422
PspPPI RGGWCCY 1 cut(s) 853
PsrI GAACNNNNNNTAC 3 cut(s) 2575, 2607, 3048
PsuI RGATCY 3 cut(s) 1546, 2177, 2360
PsyI GACNNNGTC 1 cut(s) 2035
PvuII CAGCTG 1 cut(s) 2852
RsaI GTAC 8 cut(s) 515, 1350, 1362, 1688, 2021, 2410, 2536, 2578
RsaNI GTAC 8 cut(s) 514, 1349, 1361, 1687, 2020, 2409, 2535, 2577
RseI CAYNNNNRTG 2 cut(s) 2765, 2948
SacI GAGCTC 1 cut(s) 333
SalI GTCGAC 1 cut(s) 1996
SapI GCTCTTC 2 cut(s) 226, 348
SaqAI TTAA 7 cut(s) 305, 825, 1454, 1704, 1863, 2201, 2652
SatI GCNGC 6 cut(s) 1374, 1377, 1752, 2587, 2684, 2850
Sau96I GGNCC 5 cut(s) 853, 1051, 1258, 1619, 2422
ScaI AGTACT 1 cut(s) 2578
SchI GAGTC 6 cut(s) 619, 1310, 2380, 2447, 2561, 2999
ScrFI CCNGG 6 cut(s) 117, 719, 1165, 1403, 1724, 2185
SduI GDGCHC 3 cut(s) 221, 333, 2016
SfaNI GCATC 8 cut(s) 76, 367, 1113, 1388, 1649, 1738, 1796, 2836
SfcI CTRYAG 2 cut(s) 1364, 2505
SinI GGWCC 4 cut(s) 853, 1051, 1258, 1619
SmiMI CAYNNNNRTG 2 cut(s) 2765, 2948
SmlI CTYRAG 3 cut(s) 304, 332, 834
SmoI CTYRAG 3 cut(s) 304, 332, 834
SspMI CTAG 9 cut(s) 857, 1127, 1716, 1925, 1977, 2091, 2388, 2406, 2687
SstI GAGCTC 1 cut(s) 333
StyD4I CCNGG 6 cut(s) 115, 717, 1163, 1401, 1722, 2183
StyI CCWWGG 4 cut(s) 1062, 1293, 1431, 2530
TaiI ACGT 8 cut(s) 923, 1236, 1539, 1993, 2003, 2345, 2658, 2821
TaqII GACCGA 3 cut(s) 35, 1636, 1695
TatI WGTACW 3 cut(s) 1360, 2408, 2576
TauI GCSGC 3 cut(s) 1376, 1379, 2686
TfiI GAWTC 9 cut(s) 577, 830, 1186, 1459, 1827, 2066, 2445, 2468, 2934
Tru1I TTAA 7 cut(s) 305, 825, 1454, 1704, 1863, 2201, 2652
Tru9I TTAA 7 cut(s) 305, 825, 1454, 1704, 1863, 2201, 2652
TscAI CASTG 2 cut(s) 254, 1888
TseFI GTSAC 2 cut(s) 145, 2372
TseI GCWGC 3 cut(s) 1751, 2586, 2849
Tsp45I GTSAC 2 cut(s) 145, 2372
TspDTI ATGAA 8 cut(s) 142, 429, 1328, 1524, 2127, 2247, 2693, 2874
TspGWI ACGGA 4 cut(s) 1539, 1920, 2161, 2730
TspRI CASTG 2 cut(s) 254, 1888
Tth111I GACNNNGTC 1 cut(s) 2035
Van91I CCANNNNNTGG 2 cut(s) 1228, 2698
Vha464I CTTAAG 1 cut(s) 304
VpaK11BI GGWCC 4 cut(s) 853, 1051, 1258, 1619
XagI CCTNNNNNAGG 1 cut(s) 1292
XapI RAATTY 5 cut(s) 804, 1331, 1467, 2254, 2625
XceI RCATGY 6 cut(s) 136, 1361, 1415, 1876, 2541, 2782
XcmI CCANNNNNNNNNTGG 2 cut(s) 406, 1201
XmiI GTMKAC 2 cut(s) 1815, 1997
XmnI GAANNNNTTC 2 cut(s) 603, 1593
XspI CTAG 9 cut(s) 857, 1127, 1716, 1925, 1977, 2091, 2388, 2406, 2687
ZraI GACGTC 1 cut(s) 1234
ZrmI AGTACT 1 cut(s) 2578
Zsp2I ATGCAT 1 cut(s) 2965
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.