pycom15g04420

Histidine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009350
Physical Location & Seq
Reverse (-)
2644007 .. 2648037
4031 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g04420.1

Sequence Viewer

Length: 3015 bp
ATGGTTTGGGATATGGAGACCGACAACATCGACGACATGGACATGGAAGTCCTCCGTTCCATGTGGCCTGACGACATCGGAGCTGATGGCGGGAAGCAATTCAACATGGACAACCCGGGGCGAGATGAGGACATGTTGGAGGAGGTGACATTCTTAGAGGAACCAACAATCGTTGATTTCCAGCGTCTTGTGGAGCTGACAAACTACACCGATAAGGGCTCTTCGCAACTTGCATTCCTCGTAAAACACTGGGAGTATAAGCAGGCAAACGCCGTGCGCCTTCTCAGAGAAGAACTCGACAGCTTAAGCAAGCAGAGGCAGGAAGTTGAGCTCAAGAAGTTGGAGATATTGGAAGACCATCTCTTCGAGTCAGAGAGTTATGGAGGTGACAAACGCCCCATTTCGATTTTGGATGAAGTTTATGAGATATGGCCGGAAATTGCCCCGAAGAAACATGACGTTGTTGTGCAGACAAAGAAGGTCGAGGTAGAACCCGAGTATGATACTGTTGCGTACTGGAAGCAGAGGGCAATGCAATTGGAGAAAGTGTTGGAGGCGAGCGCCGAGAGAGAGCAGATTCTTGTGGAAAAATTGCAGGAAAGCATTCAGAGTCTAGAAAGTCAGTCATCGCCGGTCGAAGAGCTGTCTCAGATTTTGAAAAGAGCTGATAATTTCTTGCATTTTGTTCTTCAGAATGCACCTGTTGTAATCGGCCACCAGGACATTATAGGAAGAACAGATGTTGAAATTTTTTCTGGAGCCGGCGTCAAAGAAGCTCAGGATTTCAAGAAAGAGGTTCTTGAGAAAGGCATACCTGCAAAAAGGGAGATGACATTTGAAACGCCCTTGTTCGGGACGAAAACGTTTTTGATATATGTAGAACCTGTCTTCAGCAAGGCAGGAGAAACAATTGGTATAAATTACATGGGGATGGATGTCACCGATCAGGTGAGGAAAAGAGAGAAGATGGCAAAGCTTCGAGAAGAGATCGCAGTCCAGAAAGCCAAGGAAACAGAACTGAACAAAACAATTCACATCACAGACGAGACAATGCGAGCGAAACAGATGCTGGCAACAATGTCTCATGAGATAAGATCACCTCTTTCCGGGGTTGTTAGCATGGCTGAGATTCTCACCACCACAAAACTTGATCGCGAGCAACGCCAGCTTTTGGACGTCATGTTATCTTCGGGAGATTTGGTCCTTCAACTGATAAACGACATCCTTGACCTTTCCAAGGTCGAGTCAGGAGTAATGAAGTTGGAAGCTACAAAATTCCGGCCACGAGAGGTAGTAAGGCACGTACTACAGACTGCGGCAGCATCGTTGCAGAAAATTTTGACCCTGGAAGGGCATGTATCTGAGGATGTTCCTGTGGAGGTCATTGGCGACGTTTTAAGAATTCGTCAAATCCTAACCAATTTGATCAGTAATGCAATCAAGTTTACTCATGAAGGCAAAGTTGGGATAAAACTTTACGTGGTATCGGACCCATCTTTTGAAGAGGACGAAGGATGCCCTCAAAACCCGAATACAGTTCAATCGACGGTTTCAGAGAATGGACCGGAAGCAGAGAAACCAACATCTCAAACTTCTTGTGACCAACAAGGTTTCGATGGAGACGGACCACACCAAAATCACTCACTTAACGACGAACCTGGAACCCCGGGTAAGCGTGAAGTTTCGATGGATGCAGCAGATCAAGAGGATGAACCTCGTTTGCCCGAAACGAAAACGGTTTGGTTACGATGTGATGTCTACGACACCGGAATCGGAATACCGGAAAATGCTTTACCTTCTCTGTTCAAAAGATACATGCAAGTCAGTGCTGATCATGCTCGAAAATATGGAGGGACAGGATTAGGACTAGCAATATGCAAACAACTGGTTGAGCTAATGGGGGGTCAGCTCACAGTATCTAGCAAAGAAAAATGTGGGTCGACATTTACGTTTGTCCTACCGTACAAGGTTTCGACATCATCCGAAAAACATTCCGACGAGCCCGATGATTCTGATGAAGCATCGGATATAGCGAGTCAAGACGGTGCAACTGATGAAGTAGCCGACAGCTATTTCCAGTTCCAGCCGCGAACTTTAGGTTCTCTGTTCTCTTCTAATGGAGCTGCCAGGACCCAAAACTTATTACCCCACAAAATGGGGTTTACCGGTTCACATAAACTCGATGGGTTTGCACGAAACTCATACTCGTTTCCAAACGGTAGCATCGGATCGGAGGAGATAGCTTTGGTTGAAAAATCTTGCCCAGAAGGTGATTTTGCCGAGACATTATCAGAACCAAAGCGCTCCTCAGCGAGCCACATCCGAGATCATAATAGTGAAAATCCAGCTAGTACAAACAAACAGGGTCGAGATGACATGGATAATCGACTCCAAGACTATAGAAGTACAAATTCAAGTCATCCTTCAGAGGAAAGAAGAGAAACAGCTGTAGCAGCACCCACAAGAGAACCCCAAGGAACATGCAAGAGGCACGAGAAACCCGACGCAGACTCAGAGAGTACTAGCAGCAGTTCAAATTTACCAAAACCAACATCGAAACCTAGGATTCTTCTTGTTGAAGATAACAAGATTAACGTTATGGTGGCACAGAACATGATGAAACGGTTAGGCCATAGCATTGATGTTGCGAACAATGGAATGGAAGCTGTGCGTGCAGTTCAACGCTGTAGATACAATCTCATCTTGATGGATGTTCACATGCCAGTTATGGATGGCCTTCAAACTACAAGAATTATACGTTCTTTCGAAGAAACTGGCAACTGGGATGCCGCTGAGAAAGCTGGAATTGAGCTTCCTCTGCCTTCATCAGAAACATTACAGAACGGTCATGGTTGCACACCTTCTGGAAAGCGAATCCCCATCATAGCGATGACAGCAAATGCATTTTCAGAGAGTGCAGAAGAATGTTTCAATAGTGGAATGGACTCGTTCGTGCCAAAGCCCGTAACATTCCAAAAATTGAAAGACCGTCTCGAACAATACCTACCCGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000302 GO:0002237 GO:0003674 GO:0003824 GO:0004672 GO:0004673 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009617 GO:0009636 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009755 GO:0009787 GO:0009788 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010104 GO:0010105 GO:0010119 GO:0010646 GO:0010648 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016775 GO:0018106 GO:0018193 GO:0018202 GO:0019538 GO:0022622 GO:0023051 GO:0023052 GO:0023057 GO:0032501 GO:0032502 GO:0032870 GO:0033554 GO:0034599 GO:0034614 GO:0035690 GO:0036211 GO:0042221 GO:0042493 GO:0042542 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046677 GO:0048364 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0070297 GO:0070298 GO:0070301 GO:0070887 GO:0071216 GO:0071219 GO:0071236 GO:0071241 GO:0071310 GO:0071368 GO:0071495 GO:0071704 GO:0071731 GO:0071732 GO:0071944 GO:0090333 GO:0097237 GO:0097366 GO:0099402 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902170 GO:1902531 GO:1902532 GO:1905957 GO:1905958
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HATPase_c HisKA Response_reg

Protein Analysis

1005

Amino Acids

112.27

Weight (kDa)

4.93

Isoelectric Point (pI)

50.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HisKA PF00512 354 - 417 1.9e-18 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 579 - 655 1.8e-20 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Response_reg PF00072 856 - 923 3.4e-13 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 47
AatII GACGTC 1 cut(s) 1179
Acc36I ACCTGC 1 cut(s) 823
AccB7I CCANNNNNTGG 2 cut(s) 1171, 2155
AccI GTMKAC 2 cut(s) 1758, 1940
AccII CGCG 2 cut(s) 1155, 2089
AciI CCGC 4 cut(s) 90, 1316, 2087, 2790
AclI AACGTT 2 cut(s) 863, 2595
AclWI GGATC 1 cut(s) 2236
AcoI YGGCCR 3 cut(s) 431, 712, 1280
AcsI RAATTY 6 cut(s) 747, 1274, 1335, 1401, 2410, 2536
AcuI CTGAAG 3 cut(s) 674, 874, 2409
AcyI GRCGYC 2 cut(s) 765, 1176
AfaI GTAC 6 cut(s) 515, 1305, 1964, 2353, 2407, 2521
AfeI AGCGCT 1 cut(s) 2303
AfiI CCNNNNNNNGG 7 cut(s) 851, 852, 1106, 1171, 1237, 1350, 2155
AflII CTTAAG 1 cut(s) 304
AflIII ACRYGT 1 cut(s) 132
AgeI ACCGGT 1 cut(s) 2165
AjnI CCWGG 4 cut(s) 717, 1344, 1657, 2126
AjuI GAANNNNNNNTTGG 2 cut(s) 1446, 1478
Alw21I GWGCWC 1 cut(s) 333
Alw26I GTCTC 7 cut(s) 11, 651, 1040, 1086, 1614, 2276, 2996
AlwI GGATC 1 cut(s) 2236
AlwNI CAGNNNCTG 1 cut(s) 1069
Ama87I CYCGRG 3 cut(s) 115, 494, 1666
Aor51HI AGCGCT 1 cut(s) 2303
AoxI GGCC 6 cut(s) 65, 431, 712, 1280, 2629, 2734
ApeKI GCWGC 5 cut(s) 1319, 1694, 2123, 2453, 2526
ApoI RAATTY 6 cut(s) 747, 1274, 1335, 1401, 2410, 2536
ArsI GACNNNNNNTTYG 4 cut(s) 347, 379, 1481, 1513
AsiGI ACCGGT 1 cut(s) 2165
Asp700I GAANNNNTTC 2 cut(s) 98, 603
AspA2I CCTAGG 1 cut(s) 2561
AspLEI GCGC 3 cut(s) 279, 563, 2304
AspS9I GGNCC 5 cut(s) 1201, 1489, 1562, 1625, 2130
AsuC2I CCSGG 5 cut(s) 116, 117, 1108, 1667, 1668
AsuHPI GGTGA 7 cut(s) 157, 398, 931, 961, 1089, 1126, 2282
AsuII TTCGAA 1 cut(s) 2766
AvaI CYCGRG 3 cut(s) 115, 494, 1666
AvaII GGWCC 5 cut(s) 1201, 1489, 1562, 1625, 2130
AvrII CCTAGG 1 cut(s) 2561
BanII GRGCYC 3 cut(s) 221, 333, 2004
BauI CACGAG 2 cut(s) 1284, 2492
BbsI GAAGAC 2 cut(s) 360, 880
Bbv12I GWGCWC 1 cut(s) 333
BbvCI CCTCAGC 1 cut(s) 2308
BbvI GCAGC 5 cut(s) 1331, 1706, 2110, 2465, 2538
BceAI ACGGC 1 cut(s) 257
BcgI CGANNNNNNTGC 4 cut(s) 1048, 1082, 2171, 2205
BciT130I CCWGG 4 cut(s) 719, 1346, 1659, 2128
BclI TGATCA 2 cut(s) 1425, 1831
BcnI CCSGG 5 cut(s) 116, 117, 1108, 1667, 1668
BcoDI GTCTC 7 cut(s) 11, 651, 1040, 1086, 1614, 2276, 2996
BfaI CTAG 6 cut(s) 614, 1868, 1920, 2349, 2523, 2562
BfmI CTRYAG 4 cut(s) 1307, 2398, 2448, 2686
BfoI RGCGCY 2 cut(s) 564, 2305
BfrI CTTAAG 1 cut(s) 304
BfuAI ACCTGC 1 cut(s) 823
BisI GCNGC 8 cut(s) 1317, 1320, 1695, 2087, 2124, 2454, 2527, 2790
BlnI CCTAGG 1 cut(s) 2561
BlsI GCNGC 8 cut(s) 1318, 1321, 1696, 2088, 2125, 2455, 2528, 2791
BmcAI AGTACT 1 cut(s) 2521
Bme1390I CCNGG 9 cut(s) 116, 117, 719, 1108, 1346, 1659, 1667, 1668, 2128
Bme18I GGWCC 5 cut(s) 1201, 1489, 1562, 1625, 2130
BmeT110I CYCGRG 3 cut(s) 115, 494, 1666
BmgT120I GGNCC 5 cut(s) 1201, 1489, 1562, 1625, 2130
BmiI GGNNCC 5 cut(s) 162, 760, 1491, 1663, 2132
BmrFI CCNGG 9 cut(s) 116, 117, 719, 1108, 1346, 1659, 1667, 1668, 2128
BmrI ACTGGG 2 cut(s) 259, 2791
BmsI GCATC 7 cut(s) 1056, 1331, 1505, 1681, 2030, 2232, 2776
BmuI ACTGGG 2 cut(s) 259, 2791
BpiI GAAGAC 2 cut(s) 360, 880
BplI GAGNNNNNCTC 2 cut(s) 279, 311
BpmI CTGGAG 1 cut(s) 777
Bpu10I CCTNAGC 2 cut(s) 777, 2308
Bpu14I TTCGAA 1 cut(s) 2766
BpuEI CTTGAG 2 cut(s) 317, 821
BpuMI CCSGG 5 cut(s) 116, 117, 1108, 1667, 1668
BsaAI YACGTR 2 cut(s) 1303, 1480
BsaHI GRCGYC 2 cut(s) 765, 1176
BsaI GGTCTC 1 cut(s) 11
BsaWI WCCGGW 4 cut(s) 1564, 1766, 1780, 2165
BsaXI ACNNNNNCTCC 6 cut(s) 533, 563, 750, 780, 1185, 1215
Bsc4I CCNNNNNNNGG 7 cut(s) 851, 852, 1106, 1171, 1237, 1350, 2155
Bse118I RCCGGY 3 cut(s) 631, 761, 2165
Bse1I ACTGG 7 cut(s) 254, 521, 1890, 2077, 2723, 2779, 2786
Bse3DI GCAATG 1 cut(s) 537
BseBI CCWGG 4 cut(s) 719, 1346, 1659, 2128
BseLI CCNNNNNNNGG 7 cut(s) 851, 852, 1106, 1171, 1237, 1350, 2155
BseMI GCAATG 1 cut(s) 537
BseMII CTCAG 8 cut(s) 298, 662, 791, 1116, 1353, 2322, 2526, 2784
BseNI ACTGG 7 cut(s) 254, 521, 1890, 2077, 2723, 2779, 2786
BseRI GAGGAG 3 cut(s) 155, 2249, 2296
BseXI GCAGC 5 cut(s) 1331, 1706, 2110, 2465, 2538
BsgI GTGCAG 3 cut(s) 488, 2694, 2937
Bsh1236I CGCG 2 cut(s) 1155, 2089
Bsh1285I CGRYCG 1 cut(s) 636
BshFI GGCC 6 cut(s) 67, 433, 714, 1282, 2631, 2736
BshTI ACCGGT 1 cut(s) 2165
BsiEI CGRYCG 1 cut(s) 636
BsiHKAI GWGCWC 1 cut(s) 333
BsiHKCI CYCGRG 3 cut(s) 115, 494, 1666
BslFI GGGAC 2 cut(s) 868, 1867
BslI CCNNNNNNNGG 7 cut(s) 851, 852, 1106, 1171, 1237, 1350, 2155
BsmAI GTCTC 7 cut(s) 11, 651, 1040, 1086, 1614, 2276, 2996
BsmBI CGTCTC 2 cut(s) 1614, 2996
BsmFI GGGAC 2 cut(s) 868, 1867
BsmI GAATGC 3 cut(s) 233, 603, 700
BsnI GGCC 6 cut(s) 67, 433, 714, 1282, 2631, 2736
Bso31I GGTCTC 1 cut(s) 11
BsoBI CYCGRG 3 cut(s) 115, 494, 1666
Bsp119I TTCGAA 1 cut(s) 2766
Bsp1286I GDGCHC 3 cut(s) 221, 333, 2004
Bsp143I GATC 9 cut(s) 943, 987, 1094, 1150, 1425, 1699, 1831, 2228, 2326
Bsp68I TCGCGA 1 cut(s) 1155
BspACI CCGC 4 cut(s) 90, 1316, 2087, 2790
BspANI GGCC 6 cut(s) 67, 433, 714, 1282, 2631, 2736
BspCNI CTCAG 8 cut(s) 297, 661, 790, 1117, 1354, 2321, 2525, 2785
BspFNI CGCG 2 cut(s) 1155, 2089
BspHI TCATGA 2 cut(s) 1084, 1450
BspLI GGNNCC 5 cut(s) 162, 760, 1491, 1663, 2132
BspMI ACCTGC 1 cut(s) 823
BspPI GGATC 1 cut(s) 2236
BspQI GCTCTTC 2 cut(s) 226, 633
BspT104I TTCGAA 1 cut(s) 2766
BspTI CTTAAG 1 cut(s) 304
BspTNI GGTCTC 1 cut(s) 11
BsrDI GCAATG 1 cut(s) 537
BsrFI RCCGGY 3 cut(s) 631, 761, 2165
BsrI ACTGG 7 cut(s) 254, 521, 1890, 2077, 2723, 2779, 2786
BssAI RCCGGY 3 cut(s) 631, 761, 2165
BssMI GATC 9 cut(s) 943, 987, 1094, 1150, 1425, 1699, 1831, 2228, 2326
BssNI GRCGYC 2 cut(s) 765, 1176
BssSI CACGAG 2 cut(s) 1284, 2492
BssT1I CCWWGG 4 cut(s) 1005, 1236, 2473, 2561
Bst2BI CACGAG 2 cut(s) 1284, 2492
Bst2UI CCWGG 4 cut(s) 719, 1346, 1659, 2128
Bst6I CTCTTC 7 cut(s) 226, 368, 633, 978, 1497, 2116, 2431
BstACI GRCGYC 2 cut(s) 765, 1176
BstAFI CTTAAG 1 cut(s) 304
BstBAI YACGTR 2 cut(s) 1303, 1480
BstBI TTCGAA 1 cut(s) 2766
BstDEI CTNAG 9 cut(s) 154, 284, 648, 777, 1125, 1362, 2308, 2512, 2793
BstENI CCTNNNNNAGG 1 cut(s) 1235
BstFNI CGCG 2 cut(s) 1155, 2089
BstH2I RGCGCY 2 cut(s) 564, 2305
BstHHI GCGC 3 cut(s) 279, 563, 2304
BstKTI GATC 9 cut(s) 946, 990, 1097, 1153, 1428, 1702, 1834, 2231, 2329
BstMAI GTCTC 7 cut(s) 11, 651, 1040, 1086, 1614, 2276, 2996
BstMBI GATC 9 cut(s) 943, 987, 1094, 1150, 1425, 1699, 1831, 2228, 2326
BstMCI CGRYCG 1 cut(s) 636
BstMWI GCNNNNNNNGC 8 cut(s) 1161, 1165, 1835, 2453, 2672, 2798, 2818, 2894
BstNI CCWGG 4 cut(s) 719, 1346, 1659, 2128
BstNSI RCATGY 5 cut(s) 136, 1358, 1819, 2484, 2722
BstSCI CCNGG 9 cut(s) 114, 115, 717, 1106, 1344, 1657, 1665, 1666, 2126
BstSFI CTRYAG 4 cut(s) 1307, 2398, 2448, 2686
BstUI CGCG 2 cut(s) 1155, 2089
BstV1I GCAGC 5 cut(s) 1331, 1706, 2110, 2465, 2538
BstV2I GAAGAC 2 cut(s) 360, 880
BsuRI GGCC 6 cut(s) 67, 433, 714, 1282, 2631, 2736
BtgZI GCGATG 2 cut(s) 612, 2903
BtsIMutI CAGTG 2 cut(s) 247, 1831
BtuMI TCGCGA 1 cut(s) 1155
BveI ACCTGC 1 cut(s) 823
CaiI CAGNNNCTG 1 cut(s) 1069
CciI TCATGA 2 cut(s) 1084, 1450
CfoI GCGC 3 cut(s) 279, 563, 2304
Cfr10I RCCGGY 3 cut(s) 631, 761, 2165
Cfr13I GGNCC 5 cut(s) 1201, 1489, 1562, 1625, 2130
Cfr9I CCCGGG 2 cut(s) 115, 1666
CseI GACGC 3 cut(s) 173, 754, 2513
Csp6I GTAC 6 cut(s) 514, 1304, 1963, 2352, 2406, 2520
CspAI ACCGGT 1 cut(s) 2165
CspCI CAANNNNNGTGG 2 cut(s) 1129, 1164
CviQI GTAC 6 cut(s) 514, 1304, 1963, 2352, 2406, 2520
DdeI CTNAG 9 cut(s) 154, 284, 648, 777, 1125, 1362, 2308, 2512, 2793
DpnI GATC 9 cut(s) 945, 989, 1096, 1152, 1427, 1701, 1833, 2230, 2328
DpnII GATC 9 cut(s) 943, 987, 1094, 1150, 1425, 1699, 1831, 2228, 2326
DrdI GACNNNNNNGTC 1 cut(s) 47
DseDI GACNNNNNNGTC 1 cut(s) 47
EaeI YGGCCR 3 cut(s) 431, 712, 1280
Eam1104I CTCTTC 7 cut(s) 226, 368, 633, 978, 1497, 2116, 2431
EarI CTCTTC 7 cut(s) 226, 368, 633, 978, 1497, 2116, 2431
Ecl136II GAGCTC 1 cut(s) 331
Eco130I CCWWGG 4 cut(s) 1005, 1236, 2473, 2561
Eco24I GRGCYC 3 cut(s) 221, 333, 2004
Eco31I GGTCTC 1 cut(s) 11
Eco47I GGWCC 5 cut(s) 1201, 1489, 1562, 1625, 2130
Eco47III AGCGCT 1 cut(s) 2303
Eco53kI GAGCTC 1 cut(s) 331
Eco57I CTGAAG 3 cut(s) 674, 874, 2409
Eco88I CYCGRG 3 cut(s) 115, 494, 1666
EcoICRI GAGCTC 1 cut(s) 331
EcoNI CCTNNNNNAGG 1 cut(s) 1235
EcoO109I RGGNCCY 1 cut(s) 2130
EcoRI GAATTC 1 cut(s) 1401
EcoRII CCWGG 4 cut(s) 717, 1344, 1657, 2126
EcoT14I CCWWGG 4 cut(s) 1005, 1236, 2473, 2561
EcoT22I ATGCAT 1 cut(s) 2905
EcoT38I GRGCYC 3 cut(s) 221, 333, 2004
ErhI CCWWGG 4 cut(s) 1005, 1236, 2473, 2561
Esp3I CGTCTC 2 cut(s) 1614, 2996
FalI AAGNNNNNCTT 4 cut(s) 783, 815, 2407, 2439
FaqI GGGAC 2 cut(s) 868, 1867
FauI CCCGC 1 cut(s) 83
FbaI TGATCA 2 cut(s) 1425, 1831
FblI GTMKAC 2 cut(s) 1758, 1940
Fnu4HI GCNGC 8 cut(s) 1317, 1320, 1695, 2087, 2124, 2454, 2527, 2790
FriOI GRGCYC 3 cut(s) 221, 333, 2004
Fsp4HI GCNGC 8 cut(s) 1317, 1320, 1695, 2087, 2124, 2454, 2527, 2790
FspBI CTAG 6 cut(s) 614, 1868, 1920, 2349, 2523, 2562
GlaI GCGC 3 cut(s) 278, 562, 2303
GluI GCNGC 8 cut(s) 1317, 1320, 1695, 2087, 2124, 2454, 2527, 2790
GsuI CTGGAG 1 cut(s) 777
HaeII RGCGCY 2 cut(s) 564, 2305
HaeIII GGCC 6 cut(s) 67, 433, 714, 1282, 2631, 2736
HgaI GACGC 3 cut(s) 173, 754, 2513
HhaI GCGC 3 cut(s) 279, 563, 2304
Hin1I GRCGYC 2 cut(s) 765, 1176
Hin6I GCGC 3 cut(s) 277, 561, 2302
HinP1I GCGC 3 cut(s) 277, 561, 2302
HincII GTYRAC 1 cut(s) 1941
HindII GTYRAC 1 cut(s) 1941
HindIII AAGCTT 1 cut(s) 974
HphI GGTGA 7 cut(s) 157, 398, 931, 961, 1089, 1126, 2282
Hpy166II GTNNAC 6 cut(s) 1446, 1759, 1941, 2163, 2171, 2716
Hpy8I GTNNAC 6 cut(s) 1446, 1759, 1941, 2163, 2171, 2716
Hpy99I CGWCG 6 cut(s) 35, 1394, 1549, 1655, 2000, 2507
HpyCH4IV ACGT 9 cut(s) 459, 863, 1176, 1302, 1392, 1479, 1949, 2595, 2758
HpyF10VI GCNNNNNNNGC 8 cut(s) 1161, 1165, 1835, 2453, 2672, 2798, 2818, 2894
HpyF3I CTNAG 9 cut(s) 154, 284, 648, 777, 1125, 1362, 2308, 2512, 2793
HpySE526I ACGT 9 cut(s) 459, 863, 1176, 1302, 1392, 1479, 1949, 2595, 2758
Hsp92I GRCGYC 2 cut(s) 765, 1176
HspAI GCGC 3 cut(s) 277, 561, 2302
KroI GCCGGC 1 cut(s) 761
KroNI GCCGGC 1 cut(s) 763
Ksp22I TGATCA 2 cut(s) 1425, 1831
Kzo9I GATC 9 cut(s) 943, 987, 1094, 1150, 1425, 1699, 1831, 2228, 2326
LguI GCTCTTC 2 cut(s) 226, 633
LmnI GCTCC 5 cut(s) 80, 193, 758, 2120, 2309
Lsp1109I GCAGC 5 cut(s) 1331, 1706, 2110, 2465, 2538
LweI GCATC 7 cut(s) 1056, 1331, 1505, 1681, 2030, 2232, 2776
MaeI CTAG 6 cut(s) 614, 1868, 1920, 2349, 2523, 2562
MaeII ACGT 9 cut(s) 459, 863, 1176, 1302, 1392, 1479, 1949, 2595, 2758
MaeIII GTNAC 6 cut(s) 145, 386, 937, 1598, 1743, 2965
MalI GATC 9 cut(s) 945, 989, 1096, 1152, 1427, 1701, 1833, 2230, 2328
MboI GATC 9 cut(s) 943, 987, 1094, 1150, 1425, 1699, 1831, 2228, 2326
MfeI CAATTG 2 cut(s) 536, 909
MhlI GDGCHC 3 cut(s) 221, 333, 2004
MlyI GAGTC 7 cut(s) 377, 619, 1253, 2044, 2382, 2504, 2939
MmeI TCCRAC 5 cut(s) 117, 321, 531, 1242, 2019
Mph1103I ATGCAT 1 cut(s) 2905
MroNI GCCGGC 1 cut(s) 761
MroXI GAANNNNTTC 2 cut(s) 98, 603
MseI TTAA 4 cut(s) 305, 1397, 1647, 2592
MslI CAYNNNNRTG 5 cut(s) 41, 929, 2334, 2705, 2888
MspA1I CMGCKG 2 cut(s) 2447, 2792
MspCI CTTAAG 1 cut(s) 304
MspR9I CCNGG 9 cut(s) 116, 117, 719, 1108, 1346, 1659, 1667, 1668, 2128
MunI CAATTG 2 cut(s) 536, 909
Mva1269I GAATGC 3 cut(s) 233, 603, 700
MvaI CCWGG 4 cut(s) 719, 1346, 1659, 2128
MvnI CGCG 2 cut(s) 1155, 2089
MwoI GCNNNNNNNGC 8 cut(s) 1161, 1165, 1835, 2453, 2672, 2798, 2818, 2894
NaeI GCCGGC 1 cut(s) 763
NciI CCSGG 5 cut(s) 116, 117, 1108, 1667, 1668
NdeII GATC 9 cut(s) 943, 987, 1094, 1150, 1425, 1699, 1831, 2228, 2326
NgoMIV GCCGGC 1 cut(s) 761
NlaIV GGNNCC 5 cut(s) 162, 760, 1491, 1663, 2132
NmeAIII GCCGAG 2 cut(s) 589, 2305
NmuCI GTSAC 4 cut(s) 145, 386, 937, 1598
NruI TCGCGA 1 cut(s) 1155
NsiI ATGCAT 1 cut(s) 2905
NspI RCATGY 5 cut(s) 136, 1358, 1819, 2484, 2722
NspV TTCGAA 1 cut(s) 2766
PagI TCATGA 2 cut(s) 1084, 1450
PciI ACATGT 1 cut(s) 132
PciSI GCTCTTC 2 cut(s) 226, 633
PcsI WCGNNNNNNNCGW 3 cut(s) 1946, 2030, 2499
PctI GAATGC 3 cut(s) 233, 603, 700
PdiI GCCGGC 1 cut(s) 763
PdmI GAANNNNTTC 2 cut(s) 98, 603
PfeI GAWTC 6 cut(s) 577, 1129, 1770, 2009, 2566, 2874
PflMI CCANNNNNTGG 2 cut(s) 1171, 2155
PinAI ACCGGT 1 cut(s) 2165
PkrI GCNGC 8 cut(s) 1318, 1321, 1696, 2088, 2125, 2455, 2528, 2791
PleI GAGTC 7 cut(s) 376, 618, 1252, 2043, 2382, 2504, 2939
PpsI GAGTC 7 cut(s) 376, 618, 1252, 2043, 2382, 2504, 2939
Ppu21I YACGTR 2 cut(s) 1303, 1480
PpuMI RGGWCCY 1 cut(s) 2130
PscI ACATGT 1 cut(s) 132
Psp124BI GAGCTC 1 cut(s) 333
Psp1406I AACGTT 2 cut(s) 863, 2595
Psp5II RGGWCCY 1 cut(s) 2130
Psp6I CCWGG 4 cut(s) 717, 1344, 1657, 2126
PspGI CCWGG 4 cut(s) 717, 1344, 1657, 2126
PspN4I GGNNCC 5 cut(s) 162, 760, 1491, 1663, 2132
PspPI GGNCC 5 cut(s) 1201, 1489, 1562, 1625, 2130
PspPPI RGGWCCY 1 cut(s) 2130
PsrI GAACNNNNNNTAC 1 cut(s) 2988
PstNI CAGNNNCTG 1 cut(s) 1069
PvuII CAGCTG 1 cut(s) 2447
RruI TCGCGA 1 cut(s) 1155
RsaI GTAC 6 cut(s) 515, 1305, 1964, 2353, 2407, 2521
RsaNI GTAC 6 cut(s) 514, 1304, 1963, 2352, 2406, 2520
RseI CAYNNNNRTG 5 cut(s) 41, 929, 2334, 2705, 2888
SacI GAGCTC 1 cut(s) 333
SalI GTCGAC 1 cut(s) 1939
SapI GCTCTTC 2 cut(s) 226, 633
SaqAI TTAA 4 cut(s) 305, 1397, 1647, 2592
SatI GCNGC 8 cut(s) 1317, 1320, 1695, 2087, 2124, 2454, 2527, 2790
Sau3AI GATC 9 cut(s) 943, 987, 1094, 1150, 1425, 1699, 1831, 2228, 2326
Sau96I GGNCC 5 cut(s) 1201, 1489, 1562, 1625, 2130
ScaI AGTACT 1 cut(s) 2521
SchI GAGTC 7 cut(s) 377, 619, 1253, 2044, 2382, 2504, 2939
ScrFI CCNGG 9 cut(s) 116, 117, 719, 1108, 1346, 1659, 1667, 1668, 2128
SduI GDGCHC 3 cut(s) 221, 333, 2004
SfaNI GCATC 7 cut(s) 1056, 1331, 1505, 1681, 2030, 2232, 2776
SfcI CTRYAG 4 cut(s) 1307, 2398, 2448, 2686
SfuI TTCGAA 1 cut(s) 2766
SinI GGWCC 5 cut(s) 1201, 1489, 1562, 1625, 2130
SmaI CCCGGG 2 cut(s) 117, 1668
SmiMI CAYNNNNRTG 5 cut(s) 41, 929, 2334, 2705, 2888
SmlI CTYRAG 3 cut(s) 304, 332, 800
SmoI CTYRAG 3 cut(s) 304, 332, 800
SsiI CCGC 4 cut(s) 90, 1316, 2087, 2790
SspMI CTAG 6 cut(s) 614, 1868, 1920, 2349, 2523, 2562
SstI GAGCTC 1 cut(s) 333
StyD4I CCNGG 9 cut(s) 114, 115, 717, 1106, 1344, 1657, 1665, 1666, 2126
StyI CCWWGG 4 cut(s) 1005, 1236, 2473, 2561
TaiI ACGT 9 cut(s) 462, 866, 1179, 1305, 1395, 1482, 1952, 2598, 2761
TaqII GACCGA 1 cut(s) 35
TatI WGTACW 3 cut(s) 2351, 2405, 2519
TauI GCSGC 3 cut(s) 1319, 2089, 2792
TfiI GAWTC 6 cut(s) 577, 1129, 1770, 2009, 2566, 2874
Tru1I TTAA 4 cut(s) 305, 1397, 1647, 2592
Tru9I TTAA 4 cut(s) 305, 1397, 1647, 2592
TscAI CASTG 2 cut(s) 254, 1831
TseFI GTSAC 4 cut(s) 145, 386, 937, 1598
TseI GCWGC 5 cut(s) 1319, 1694, 2123, 2453, 2526
Tsp45I GTSAC 4 cut(s) 145, 386, 937, 1598
TspDTI ATGAA 8 cut(s) 429, 1271, 1467, 1725, 2031, 2070, 2633, 2814
TspGWI ACGGA 2 cut(s) 44, 1638
TspMI CCCGGG 2 cut(s) 115, 1666
TspRI CASTG 2 cut(s) 254, 1831
Van91I CCANNNNNTGG 2 cut(s) 1171, 2155
Vha464I CTTAAG 1 cut(s) 304
VpaK11BI GGWCC 5 cut(s) 1201, 1489, 1562, 1625, 2130
XagI CCTNNNNNAGG 1 cut(s) 1235
XapI RAATTY 6 cut(s) 747, 1274, 1335, 1401, 2410, 2536
XbaI TCTAGA 1 cut(s) 613
XceI RCATGY 5 cut(s) 136, 1358, 1819, 2484, 2722
XcmI CCANNNNNNNNNTGG 1 cut(s) 406
XmaI CCCGGG 2 cut(s) 115, 1666
XmaJI CCTAGG 1 cut(s) 2561
XmiI GTMKAC 2 cut(s) 1758, 1940
XmnI GAANNNNTTC 2 cut(s) 98, 603
XspI CTAG 6 cut(s) 614, 1868, 1920, 2349, 2523, 2562
ZraI GACGTC 1 cut(s) 1177
ZrmI AGTACT 1 cut(s) 2521
Zsp2I ATGCAT 1 cut(s) 2905
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.