FvH4_2g38620

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0010532
Physical Location & Seq
Forward (+)
27889817 .. 27897733
7917 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g38620.t8

Sequence Viewer

Length: 5208 bp
ATGTCTGCTGCTGCTGCTGATTCTTCCTCTTCTTCTTCTTTCCGCCTTCGTGGCAAGTATGATGTCTTCTTGAGCTTTAGAGGGTTAGACACTCGCAAGATCTTTGTTGGTCATCTCTACGATGCTCTACAACGGAAAGCACTCGCGGTCTTCATAGATACTGAACAGCTTGGAAAAGGCGATGTTCTTGGGGTGCTACTGAAAGCTATCGACGCGTCAAAGCTTTCGGTGGTAGTATTGTCTGAGAACTTTGCCAATTCCAAATGGTGCTTACTGGAACTTGAGAAAATCGTGGAATGTATGGAAACAAAGAATCATATAGTGGTTCCCATCTTCTACCAAGTAGATCCTGCTGATGTTCGTTATGTGAAGGGAAAGTTTAGCAAAGCGTTTGAAGAACTTGAGCACAAGTCCAGAGGGACTGTTGAAGAACGGAAGAGGTGGAAGTCTGCTCTAACTAGAGCTGGCTACATTAATGGCTGGGATTCCCGCAAATACGAGAATGATGTTAAGCTTATTGATGATGTTGTTAAAGATATATTAAAACAGTTGAGCAACATCCGGGAAAGTAAATCCAATGATGAATTGGTTGGAATGGATTCCCACATAAGTGAAATGAAGTCTCTATTAAGTGATCATAAGCCGGGGAATGTCTGCGTTGTTGGAATATGGGGTATGGGCGGTATAGGGAAGACAACCATTGCTAGAGTTGTTTATGAGGATATGTGTGGAGACTTTGATCACCATTGCTTTCTTTATAATGTCAAGGAAGATTTCAAGAGAAAAGGTGAAGCAGGAATGCTGGAAGAACTTCTATATAGAATCTCAAGGGAAAATGGGCAGCACTTGAGTTCCAAAACGATAATGGAAAGGCTCAGTGGGATAAAACTTTTTCTTGTTCTTGATGATGTGGACAGTTCTGATCAAATTGATGCCCTACTTGGAAAACAATGTTCATTTGGTCGTGGAAGTAGAATCATTATAACGACCAGAGATAAGGGCCTACTTAGAGAATTTGATGTATATGAACCGGCATATTTAGATAAAGATCAAGCTCTTGAGCTCTTTAATAAGTATGCCTTCAGAACAACTGAACCCTCAACAGAGTACGATCATCTCTTACAGCGTGCCATAAAATATGCTCAGGGTCTGCCTTTAGCACTTAAAGTCTTGGGAGCTTCTCTCTATAAAAAAAGTGTACGTGAGTGGGAAGTTGAGTTTGAAAAAATAAGGAAATGCCAGCACCTGGGAATTGAGGGTGTACTGAGGACAACCTTCGATGGACTAGATCATCTACAGAAAACAGTACTTCTAGATATTGCATGTTTCTTTAGAGGAATGAAGAAAGACTATGCAACAGAAATTCTGGAGGCTTGTGGCTTCTTTCCCATAAGTGTATTACCAGTACTGGCTGATAGAGCTCTAGTGACTAGCACTGGATACTATGTTGTGCTGACGATGCATGATTTACTATTGGACATGGCCTTGGGAATTGTCCGTAGAGAATCAAGCCTCAGTAGGTTGACAAGCCCTGATGAAATCGAGGAGGTGTTAACTCAAATTACGGATACGAAAGCAGTTGTAAGTATAGACCTCGACTTATACCCCTTGAAAGGCATACGCATATATGCTAAAGCTTTTATTCACATGACAAAACTAAGACTGCTCAGAATCCGATATAACCGCTCCATAGACTTTGTTGATGATTATTATGGTTCACGGGGGGAATTATACCATCTAATCGATGACTGTAAACAACAAGTGAGTGGGGAGTTAAAGTTTCTTTCTCATGAGTTGAGGTGTCTCATCTGGCATGGATGCCCCCTGAAATCTTTGCCATCCAATTTCAACCCGAAGCATCTAGTTGACCTTGACATGCGTTCTAGCCACATCAAACAACTCTGGAAAGGAACTAAGCTGCTGAAAAAGCTGAAATACATTGATTTGAGTCTATCCCTATACCTTAAGGAGACGCCAGACTTAACCGAGGCCACAATTCTTGAGAAGGTAAACCTTGAAGGTTGTACAAGTTTATTGGAACTTCACCCGTCCATCTCTAATCTTAAGAACCTCCTTTTCTTGAGTCTAAAAGGGTGCAAAGAGCTCAAGATTCTTCCAAACAGCTTCTCTATGAAATCTCTTAAGGTCCTTAATTTTTCTGGCTGCTCAAATCTCGAGAAATTTCCTGAGATTTCAGAAGTTATGGCAGAGCTACCACAGCTTTGTTTAGATAAGACTGCAATTAAAGAACTGCCTTTATCAATAGAAAATCTTAGGGGGCTAGTCACTTTGAGCCTAAAAGATTGCAGAGAACTTAAGAGTCTCCCTAGCAGCATTTGTCAACTCAAATCCCTTAGATATGTTACTCTCTCTGGGTGTTCTAAGTTTAAGGTGTTTCCACAAATTGTGGGGTTAGAAGATATGAAAAGCTTAAGAGAGCTTCATTTGGATGGAACGTGTATCAAAGAGTTTCTGCCGTCAATATTAGCTCTTAAAGAACTTGTTGTTTTGAGTCTAAGAGATTGCAAAGAGCTTAAGAGTCTTCCTAATAGCATTCATATGAAATCTCTTGACACACTTATTCTTTCTGGATGTTCCAACCTTCAGAAGTTTCCAGAGATTTCAGAAAAAACGCATAGGCTCTCAGAGCTTCATTTACATGATATTGCAATTGAAGAACTGCCGCCGTCAATTACAAATCTTTGCCAGCTGGAATCTTTGATCCTTAAAGATTGTGAAAAACTTAAGAGTCTTCCAAGCAGCATTTATCAACTTGAGTGCCTCAGTACAATTACTGTCTCTGGTTGTTCAAAGTTTAAGGTGTTTCCTCAAAATACAAATTCTTTGGAAGGACTACAAAAACTAGTTACATTGAGGCTAGAAGATTGCGGAGTACTTAGGAGTCTGCCGAGCAGCATCTGTCAGCTCAAGTCCCTTAGATATGTTGCTCTCTCTGGTTGTTCAAAGTTTGAGGTGTTTCCAGAAATTTTTGAAGATATGGAAGAATTAAGAGAGCTTCATTTGGAAGGAACGTCTATCAAGTACCTTTCCCCATCAATTCAACGGCTTAAGAGGATTATGTTATTGAACATGAGTAACTGTAGAAGCCTCATCTGTCTTCCTGACAATATCTGCAATTTGTCATACCTTACAGGTCTCACTCTCTCAGGGTGCTTGAATCTCCATGACTTGCCCAAGAATTTGGGAAAATTAGAATATTTGGTGGATCTTGAAGTACAGGGAAGTGGTATAAAGCAACCGTTCTCTCTTTTATATCCAAAGAAGTCACCTTCATCATCAGCCAATTGCTTGGTTGGCATGGATCGCCATATAAAGAACATGCAAGTGCTATTATGTCCTGATCAGGTGGATGATGTTTGTGTTGTTGGAATATGTGGAATTGGTGGGATCGGCAAGACAACCATTGCTACAGCTGTATATGATGAAATTGCTTCGCAATTTGAACACCATTGTTTTCTTCAGAATGTCAAGGAAGGTTTCACAAAGCATAGTGAAGAACAAATGCAGGAAGAACTTCTGACTAGAATCTTAAAGGTGAAAGTACAGAGTTTAGGCATTTTGAATAGAGGCTCCAATATGTTTATTGAAAGGCTAGGTATGAAAAAGGTTCTGGTTGTTCTTGATGATATAGACGATAAAATCCAGATTGAAACTTTGCTTGGAAAGCCATATTCACTTGGTGGTGGGAGTCGGATCATTGTGACTACTAGAAAGGAAGAAATATTGAGTGGATTCAAAATATATCATCCCCGGCTATTAGATGATGAAGCTGTTGAACTGTTCAGACATTTTGCCTTCAGAACCATCAAACGCTCAAGAAAGTATGATGTTCTCTCACATCGTGCCTCAGAACATGCTGAAGTGCCTTTAGATCTTAAAGTCTGGGGAGCTTTTCTTTTCAACAAAAGTATACATGAGTGGGAAGATGCCTTGACAGATAAGCACACAGTTTATTGGGTGCTAACAACTCACTTTTATGGCCTACATTATTCAGAGAGGAACATATTAATAGATGTTGCATGGTTCTTTGAAGAAAGGGATGGGACTATCAAAATGCATGATTTACTGCATGGGATTGTTCGGGATTGCGTTCGCCATGAAGATGAACATGAACCTGGGAACCGCAGTAGGTTAGAGGGTTACCAGGGAATTAGGTGTCTGTCAACTCAAGATCAGGTTACTAAAGCAGTTCAAGTTATAACCCTTGATCTGTCAAACTCAAAAGAGGTATACTTCATCGCAGAGGCTTTTGTTACTATGAAGAAATTAAGACTGCTCCAAATTTATGATAACGGCTCCATTAACTGCAAACAACATCTGATTGGAGAGTTTGAGTTTATTTCTCATGAGTTGAGGTGTCTCATCTGGCATAGATGCCCCGTAAAGTCTTTGCCAAACAATTTTAACCCAACGAATCTTGTCGACCTTGACATGCGTTTTAGTCGCCTTGAACAACTTTGGGAAGGAACCAAGCCTCTGAAATACTTGACATTCATCAATTTAAGCCACTCTCAATATCTCAAGGAAGTCCCGGACTTGACCACAGCTACAAATCTTGAGAAGCTAGTTCTTGATGGTTGTACAAGTTTAATTGAGGTGCATCCATCGATTTGGGATAAAAAAAATCTCATTCTCTTGAGTCTAAAAGGTTGCAGACAACTTAAGATTCTTCCAACCAACATCCATATGAAATCTCTCAAGATCCTTGATCTTTCTGGCTGCAAGAATCTTGAGAAGTTTCCCGAGATTTCAGAGGTTATGATGGAGCTAGAAGAGCTTGGTTTAGATGAGACTGCAATTCAAAAACTTCCCTCGTCAATTGAACGGCTACAGAGGCTTAATGTATTAAGTATGAGAAACTGCACAAGTCTTTTCTCTCTTCCGTACAGCATCTGTAGTTTGGCACACCTTACATTTCTCAATCTGTCAGGGTGCTCAAAACTTTCTGACTTTCCTGACAGCTTGAGGAATAGACAGTCATTGACAATCAGTGGTATAGAAGAACTCACATTCGGTATGAGAAGCAAAGAAATAGAGAACGAGGTTTCATCAGACGAGTTCTTCAGTCTCTCCGAGAATTCATCAGAGTCGGGGGAAGAGGTTGATGGAAAAGAAGAGAAAGCGGAGATGGAGGGGGTAATGCAAGCAGATGATTGCTCCGCAAGAGGAGGGATGTTGGGTGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

1736

Amino Acids

196.87

Weight (kDa)

6.4

Isoelectric Point (pI)

38.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 19 - 198 2e-48 TIR domain
TIR_2 PF13676 22 - 127 1e-12 TIR domain
NB-ARC PF00931 202 - 364 2.2e-18 NB-ARC domain
WHD_ROQ1 PF23282 433 - 503 5.3e-11 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 703 - 871 1.8e-14 Leucine-rich repeat region
LRR_13 PF23286 712 - 797 2.4e-13 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 849 - 960 6.6e-08 Leucine-rich repeat region
LRR_13 PF23286 854 - 940 3.6e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 948 - 1081 2.5e-10 Leucine-rich repeat region
NB-ARC PF00931 1124 - 1274 6.8e-16 NB-ARC domain
WHD_ROQ1 PF23282 1338 - 1407 6.7e-13 Disease resistance protein Roq1-like, winged-helix domain
LRR_3 PF07725 1513 - 1532 1.7e-06 Leucine Rich Repeat
LRR_14 PF23598 1597 - 1702 1.9e-11 Leucine-rich repeat region
LRR_13 PF23286 1604 - 1691 3.1e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 759, 983, 4220
AccB1I GGYRCC 1 cut(s) 5201
AccB7I CCANNNNNTGG 1 cut(s) 1246
AccBSI CCGCTC 1 cut(s) 1686
AccI GTMKAC 3 cut(s) 3931, 4251, 4443
AccII CGCG 2 cut(s) 146, 215
AclWI GGATC 7 cut(s) 341, 2716, 3234, 3330, 3416, 3722, 4717
AcsI RAATTY 8 cut(s) 1013, 1362, 2180, 2839, 2985, 3199, 4302, 5098
AcuI CTGAAG 6 cut(s) 1066, 2588, 3464, 3802, 3900, 5068
AcyI GRCGYC 1 cut(s) 1973
AdeI CACNNNGTG 2 cut(s) 3701, 3863
AfiI CCNNNNNNNGG 2 cut(s) 1246, 1613
AflII CTTAAG 9 cut(s) 1964, 2063, 2141, 2315, 2431, 2534, 2744, 3068, 4682
AflIII ACRYGT 2 cut(s) 213, 2456
AhlI ACTAGT 1 cut(s) 2863
AjnI CCWGG 3 cut(s) 1245, 4135, 4164
AjuI GAANNNNNNNTTGG 2 cut(s) 3663, 3695
AleI CACNNNNGTG 1 cut(s) 609
Alw21I GWGCWC 5 cut(s) 408, 1065, 1423, 2106, 4958
AlwI GGATC 7 cut(s) 341, 2716, 3234, 3330, 3416, 3722, 4717
AlwNI CAGNNNCTG 4 cut(s) 1150, 1246, 2919, 4914
Ama87I CYCGRG 2 cut(s) 2174, 4763
AoxI GGCC 4 cut(s) 1000, 1482, 1989, 4000
ApoI RAATTY 8 cut(s) 1013, 1362, 2180, 2839, 2985, 3199, 4302, 5098
AseI ATTAAT 2 cut(s) 474, 4028
Asp700I GAANNNNTTC 3 cut(s) 598, 810, 3534
AspS9I GGNCC 2 cut(s) 1000, 2146
AsuC2I CCSGG 4 cut(s) 563, 645, 3772, 4553
AsuHPI GGTGA 5 cut(s) 734, 800, 2036, 3279, 3568
AvaI CYCGRG 2 cut(s) 2174, 4763
AvaII GGWCC 1 cut(s) 2146
BanI GGYRCC 1 cut(s) 5201
BanII GRGCYC 3 cut(s) 1065, 1423, 2106
BarI GAAGNNNNNNTAC 2 cut(s) 50, 82
BbsI GAAGAC 6 cut(s) 58, 142, 698, 2534, 2744, 3110
Bbv12I GWGCWC 5 cut(s) 408, 1065, 1423, 2106, 4958
BbvI GCAGC 7 cut(s) 853, 1905, 2150, 2343, 2772, 2925, 4727
BceAI ACGGC 5 cut(s) 2461, 2671, 3080, 4330, 4862
BciT130I CCWGG 3 cut(s) 1247, 4137, 4166
BciVI GTATCC 2 cut(s) 1433, 1561
BclI TGATCA 4 cut(s) 634, 739, 922, 3361
BcnI CCSGG 4 cut(s) 563, 645, 3772, 4553
BcuI ACTAGT 1 cut(s) 2863
BfmI CTRYAG 5 cut(s) 1295, 3100, 3429, 4850, 4915
BfrI CTTAAG 9 cut(s) 1964, 2063, 2141, 2315, 2431, 2534, 2744, 3068, 4682
BfuI GTATCC 2 cut(s) 1433, 1561
BglI GCCNNNNNGGC 1 cut(s) 51
BglII AGATCT 2 cut(s) 99, 3892
BmcAI AGTACT 3 cut(s) 1308, 1407, 2895
Bme1390I CCNGG 7 cut(s) 563, 645, 1247, 3772, 4137, 4166, 4553
Bme18I GGWCC 1 cut(s) 2146
BmeT110I CYCGRG 2 cut(s) 2174, 4763
BmgT120I GGNCC 2 cut(s) 1000, 2146
BmiI GGNNCC 6 cut(s) 327, 3592, 4142, 4318, 4489, 5203
BmrFI CCNGG 7 cut(s) 563, 645, 1247, 3772, 4137, 4166, 4553
BpiI GAAGAC 6 cut(s) 58, 142, 698, 2534, 2744, 3110
BplI GAGNNNNNCTC 2 cut(s) 1167, 1199
BpmI CTGGAG 1 cut(s) 1388
Bpu10I CCTNAGC 1 cut(s) 1143
BpuMI CCSGG 4 cut(s) 563, 645, 3772, 4553
Bsa29I ATCGAT 2 cut(s) 1743, 4628
BsaAI YACGTR 1 cut(s) 1202
BsaBI GATNNNNATC 1 cut(s) 1047
BsaHI GRCGYC 1 cut(s) 1973
BsaI GGTCTC 1 cut(s) 3161
BsaJI CCNNGG 7 cut(s) 644, 1246, 1485, 1986, 3770, 4136, 4165
BsaXI ACNNNNNCTCC 2 cut(s) 1763, 1793
Bsc4I CCNNNNNNNGG 2 cut(s) 1246, 1613
Bse118I RCCGGY 1 cut(s) 1030
Bse1I ACTGG 4 cut(s) 279, 1403, 1413, 1441
Bse3DI GCAATG 3 cut(s) 699, 745, 3423
Bse8I GATNNNNATC 1 cut(s) 1047
BseBI CCWGG 3 cut(s) 1247, 4137, 4166
BseCI ATCGAT 2 cut(s) 1743, 4628
BseDI CCNNGG 7 cut(s) 644, 1246, 1485, 1986, 3770, 4136, 4165
BseJI GATNNNNATC 1 cut(s) 1047
BseLI CCNNNNNNNGG 2 cut(s) 1246, 1613
BseMI GCAATG 3 cut(s) 699, 745, 3423
BseNI ACTGG 4 cut(s) 279, 1403, 1413, 1441
BseRI GAGGAG 2 cut(s) 1559, 5202
BseXI GCAGC 7 cut(s) 853, 1905, 2150, 2343, 2772, 2925, 4727
BseYI CCCAGC 1 cut(s) 480
BsgI GTGCAG 1 cut(s) 4867
Bsh1236I CGCG 2 cut(s) 146, 215
BshFI GGCC 4 cut(s) 1002, 1484, 1991, 4002
BshNI GGYRCC 1 cut(s) 5201
BshVI ATCGAT 2 cut(s) 1743, 4628
BsiHKAI GWGCWC 5 cut(s) 408, 1065, 1423, 2106, 4958
BsiHKCI CYCGRG 2 cut(s) 2174, 4763
BsiSI CCGG 5 cut(s) 562, 644, 1031, 3772, 4553
BslFI GGGAC 4 cut(s) 433, 2917, 4078, 4535
BslI CCNNNNNNNGG 2 cut(s) 1246, 1613
BsmBI CGTCTC 1 cut(s) 1964
BsmFI GGGAC 4 cut(s) 433, 2917, 4078, 4535
BsmI GAATGC 2 cut(s) 804, 2553
BsnI GGCC 4 cut(s) 1002, 1484, 1991, 4002
Bso31I GGTCTC 1 cut(s) 3161
BsoBI CYCGRG 2 cut(s) 2174, 4763
Bsp1286I GDGCHC 5 cut(s) 408, 1065, 1423, 2106, 4958
Bsp1407I TGTACA 2 cut(s) 2024, 4601
BspANI GGCC 4 cut(s) 1002, 1484, 1991, 4002
BspDI ATCGAT 2 cut(s) 1743, 4628
BspFNI CGCG 2 cut(s) 146, 215
BspHI TCATGA 2 cut(s) 1789, 4366
BspLI GGNNCC 6 cut(s) 327, 3592, 4142, 4318, 4489, 5203
BspPI GGATC 7 cut(s) 341, 2716, 3234, 3330, 3416, 3722, 4717
BspQI GCTCTTC 1 cut(s) 4788
BspT107I GGYRCC 1 cut(s) 5201
BspTI CTTAAG 9 cut(s) 1964, 2063, 2141, 2315, 2431, 2534, 2744, 3068, 4682
BspTNI GGTCTC 1 cut(s) 3161
BsrBI CCGCTC 1 cut(s) 1686
BsrDI GCAATG 3 cut(s) 699, 745, 3423
BsrFI RCCGGY 1 cut(s) 1030
BsrGI TGTACA 2 cut(s) 2024, 4601
BsrI ACTGG 4 cut(s) 279, 1403, 1413, 1441
BssAI RCCGGY 1 cut(s) 1030
BssECI CCNNGG 7 cut(s) 644, 1246, 1485, 1986, 3770, 4136, 4165
BssNAI GTATAC 2 cut(s) 3932, 4252
BssNI GRCGYC 1 cut(s) 1973
BssT1I CCWWGG 1 cut(s) 1485
Bst1107I GTATAC 2 cut(s) 3932, 4252
Bst2UI CCWGG 3 cut(s) 1247, 4137, 4166
Bst6I CTCTTC 6 cut(s) 34, 431, 4788, 4905, 5112, 5130
BstACI GRCGYC 1 cut(s) 1973
BstAFI CTTAAG 9 cut(s) 1964, 2063, 2141, 2315, 2431, 2534, 2744, 3068, 4682
BstAUI TGTACA 2 cut(s) 2024, 4601
BstBAI YACGTR 1 cut(s) 1202
BstC8I GCNNGC 5 cut(s) 466, 1128, 1241, 2708, 5166
BstEII GGTNACC 1 cut(s) 4160
BstFNI CGCG 2 cut(s) 146, 215
BstNI CCWGG 3 cut(s) 1247, 4137, 4166
BstNSI RCATGY 5 cut(s) 1326, 1879, 3343, 3878, 4456
BstPI GGTNACC 1 cut(s) 4160
BstSCI CCNGG 7 cut(s) 561, 643, 1245, 3770, 4135, 4164, 4551
BstSFI CTRYAG 5 cut(s) 1295, 3100, 3429, 4850, 4915
BstUI CGCG 2 cut(s) 146, 215
BstV1I GCAGC 7 cut(s) 853, 1905, 2150, 2343, 2772, 2925, 4727
BstV2I GAAGAC 6 cut(s) 58, 142, 698, 2534, 2744, 3110
BstX2I RGATCY 5 cut(s) 99, 346, 3226, 3892, 4722
BstXI CCANNNNNNTGG 3 cut(s) 3202, 3310, 4632
BstYI RGATCY 5 cut(s) 99, 346, 3226, 3892, 4722
BstZ17I GTATAC 2 cut(s) 3932, 4252
Bsu15I ATCGAT 2 cut(s) 1743, 4628
BsuI GTATCC 2 cut(s) 1433, 1561
BsuRI GGCC 4 cut(s) 1002, 1484, 1991, 4002
BsuTUI ATCGAT 2 cut(s) 1743, 4628
BtgZI GCGATG 2 cut(s) 195, 4243
BtsIMutI CAGTG 3 cut(s) 883, 1434, 5017
Cac8I GCNNGC 5 cut(s) 466, 1128, 1241, 2708, 5166
CaiI CAGNNNCTG 4 cut(s) 1150, 1246, 2919, 4914
CciI TCATGA 2 cut(s) 1789, 4366
Cfr10I RCCGGY 1 cut(s) 1030
Cfr13I GGNCC 2 cut(s) 1000, 2146
ClaI ATCGAT 2 cut(s) 1743, 4628
CseI GACGC 3 cut(s) 204, 221, 1981
CspCI CAANNNNNGTGG 2 cut(s) 1981, 2016
DraIII CACNNNGTG 2 cut(s) 3701, 3863
Eam1104I CTCTTC 6 cut(s) 34, 431, 4788, 4905, 5112, 5130
EarI CTCTTC 6 cut(s) 34, 431, 4788, 4905, 5112, 5130
EciI GGCGGA 1 cut(s) 32
Ecl136II GAGCTC 3 cut(s) 1063, 1421, 2104
Eco130I CCWWGG 1 cut(s) 1485
Eco24I GRGCYC 3 cut(s) 1065, 1423, 2106
Eco31I GGTCTC 1 cut(s) 3161
Eco47I GGWCC 1 cut(s) 2146
Eco53kI GAGCTC 3 cut(s) 1063, 1421, 2104
Eco57I CTGAAG 6 cut(s) 1066, 2588, 3464, 3802, 3900, 5068
Eco88I CYCGRG 2 cut(s) 2174, 4763
Eco91I GGTNACC 1 cut(s) 4160
EcoICRI GAGCTC 3 cut(s) 1063, 1421, 2104
EcoO109I RGGNCCY 2 cut(s) 1000, 2146
EcoO65I GGTNACC 1 cut(s) 4160
EcoRI GAATTC 1 cut(s) 5098
EcoRII CCWGG 3 cut(s) 1245, 4135, 4164
EcoT14I CCWWGG 1 cut(s) 1485
EcoT22I ATGCAT 2 cut(s) 1464, 4080
EcoT38I GRGCYC 3 cut(s) 1065, 1423, 2106
ErhI CCWWGG 1 cut(s) 1485
Esp3I CGTCTC 1 cut(s) 1964
FalI AAGNNNNNCTT 2 cut(s) 1064, 1096
FaqI GGGAC 4 cut(s) 433, 2917, 4078, 4535
FauI CCCGC 1 cut(s) 497
FauNDI CATATG 2 cut(s) 2559, 4707
FbaI TGATCA 4 cut(s) 634, 739, 922, 3361
FblI GTMKAC 3 cut(s) 3931, 4251, 4443
FriOI GRGCYC 3 cut(s) 1065, 1423, 2106
GsaI CCCAGC 1 cut(s) 484
GsuI CTGGAG 1 cut(s) 1388
HaeIII GGCC 4 cut(s) 1002, 1484, 1991, 4002
HapII CCGG 5 cut(s) 562, 644, 1031, 3772, 4553
HgaI GACGC 3 cut(s) 204, 221, 1981
Hin1I GRCGYC 1 cut(s) 1973
HincII GTYRAC 6 cut(s) 1524, 1554, 1867, 2342, 4185, 4444
HindII GTYRAC 6 cut(s) 1524, 1554, 1867, 2342, 4185, 4444
HindIII AAGCTT 4 cut(s) 221, 512, 1635, 2428
HpaI GTTAAC 1 cut(s) 1554
HpaII CCGG 5 cut(s) 562, 644, 1031, 3772, 4553
HphI GGTGA 5 cut(s) 734, 800, 2036, 3279, 3568
Hpy99I CGWCG 1 cut(s) 215
HpyCH4IV ACGT 3 cut(s) 1201, 2456, 3032
HpySE526I ACGT 3 cut(s) 1201, 2456, 3032
Hsp92I GRCGYC 1 cut(s) 1973
Ksp22I TGATCA 4 cut(s) 634, 739, 922, 3361
KspAI GTTAAC 1 cut(s) 1554
LguI GCTCTTC 1 cut(s) 4788
LmnI GCTCC 8 cut(s) 1175, 1691, 3596, 3908, 4302, 4322, 4786, 5183
Lsp1109I GCAGC 7 cut(s) 853, 1905, 2150, 2343, 2772, 2925, 4727
MaeII ACGT 3 cut(s) 1201, 2456, 3032
MbiI CCGCTC 1 cut(s) 1686
MfeI CAATTG 3 cut(s) 2670, 3304, 4839
MflI RGATCY 5 cut(s) 99, 346, 3226, 3892, 4722
MhlI GDGCHC 5 cut(s) 408, 1065, 1423, 2106, 4958
MluI ACGCGT 1 cut(s) 213
MmeI TCCRAC 6 cut(s) 571, 643, 2622, 3367, 3692, 4718
Mph1103I ATGCAT 2 cut(s) 1464, 4080
MroXI GAANNNNTTC 3 cut(s) 598, 810, 3534
MslI CAYNNNNRTG 8 cut(s) 609, 2448, 2558, 2658, 3344, 3996, 4122, 4706
MspA1I CMGCKG 2 cut(s) 2710, 3434
MspCI CTTAAG 9 cut(s) 1964, 2063, 2141, 2315, 2431, 2534, 2744, 3068, 4682
MspI CCGG 5 cut(s) 562, 644, 1031, 3772, 4553
MspR9I CCNGG 7 cut(s) 563, 645, 1247, 3772, 4137, 4166, 4553
MunI CAATTG 3 cut(s) 2670, 3304, 4839
Mva1269I GAATGC 2 cut(s) 804, 2553
MvaI CCWGG 3 cut(s) 1247, 4137, 4166
MvnI CGCG 2 cut(s) 146, 215
NciI CCSGG 4 cut(s) 563, 645, 3772, 4553
NdeI CATATG 2 cut(s) 2559, 4707
NlaIV GGNNCC 6 cut(s) 327, 3592, 4142, 4318, 4489, 5203
NmeAIII GCCGAG 1 cut(s) 2934
NmuCI GTSAC 4 cut(s) 1426, 2284, 3285, 3721
NsiI ATGCAT 2 cut(s) 1464, 4080
NspI RCATGY 5 cut(s) 1326, 1879, 3343, 3878, 4456
OliI CACNNNNGTG 1 cut(s) 609
PaeR7I CTCGAG 1 cut(s) 2174
PagI TCATGA 2 cut(s) 1789, 4366
PciSI GCTCTTC 1 cut(s) 4788
PctI GAATGC 2 cut(s) 804, 2553
PdmI GAANNNNTTC 3 cut(s) 598, 810, 3534
PflMI CCANNNNNTGG 1 cut(s) 1246
PfoI TCCNGGA 2 cut(s) 561, 4551
Ppu21I YACGTR 1 cut(s) 1202
PpuMI RGGWCCY 1 cut(s) 2146
PshBI ATTAAT 2 cut(s) 474, 4028
PsiI TTATAA 3 cut(s) 759, 983, 4220
Psp124BI GAGCTC 3 cut(s) 1065, 1423, 2106
Psp5II RGGWCCY 1 cut(s) 2146
Psp6I CCWGG 3 cut(s) 1245, 4135, 4164
PspEI GGTNACC 1 cut(s) 4160
PspFI CCCAGC 1 cut(s) 480
PspGI CCWGG 3 cut(s) 1245, 4135, 4164
PspN4I GGNNCC 6 cut(s) 327, 3592, 4142, 4318, 4489, 5203
PspPI GGNCC 2 cut(s) 1000, 2146
PspPPI RGGWCCY 1 cut(s) 2146
PstNI CAGNNNCTG 4 cut(s) 1150, 1246, 2919, 4914
PsuI RGATCY 5 cut(s) 99, 346, 3226, 3892, 4722
PvuII CAGCTG 2 cut(s) 2710, 3434
RseI CAYNNNNRTG 8 cut(s) 609, 2448, 2558, 2658, 3344, 3996, 4122, 4706
SacI GAGCTC 3 cut(s) 1065, 1423, 2106
SalI GTCGAC 1 cut(s) 4442
SapI GCTCTTC 1 cut(s) 4788
Sau96I GGNCC 2 cut(s) 1000, 2146
ScaI AGTACT 3 cut(s) 1308, 1407, 2895
ScrFI CCNGG 7 cut(s) 563, 645, 1247, 3772, 4137, 4166, 4553
SduI GDGCHC 5 cut(s) 408, 1065, 1423, 2106, 4958
SfcI CTRYAG 5 cut(s) 1295, 3100, 3429, 4850, 4915
Sfr274I CTCGAG 1 cut(s) 2174
SinI GGWCC 1 cut(s) 2146
SlaI CTCGAG 1 cut(s) 2174
SmiMI CAYNNNNRTG 8 cut(s) 609, 2448, 2558, 2658, 3344, 3996, 4122, 4706
SpeI ACTAGT 1 cut(s) 2863
SspI AATATT 3 cut(s) 2484, 3218, 3744
SstI GAGCTC 3 cut(s) 1065, 1423, 2106
StyD4I CCNGG 7 cut(s) 561, 643, 1245, 3770, 4135, 4164, 4551
StyI CCWWGG 1 cut(s) 1485
TaiI ACGT 3 cut(s) 1204, 2459, 3035
TaqI TCGA 8 cut(s) 210, 1278, 1542, 1596, 1743, 2175, 4443, 4628
TatI WGTACW 9 cut(s) 1261, 1306, 1405, 2024, 2786, 2893, 3235, 3562, 4601
TauI GCSGC 1 cut(s) 2686
TscAI CASTG 3 cut(s) 883, 1441, 5017
TseFI GTSAC 4 cut(s) 1426, 2284, 3285, 3721
Tsp45I GTSAC 4 cut(s) 1426, 2284, 3285, 3721
TspGWI ACGGA 5 cut(s) 148, 448, 1487, 1580, 4893
TspRI CASTG 3 cut(s) 883, 1441, 5017
Van91I CCANNNNNTGG 1 cut(s) 1246
Vha464I CTTAAG 9 cut(s) 1964, 2063, 2141, 2315, 2431, 2534, 2744, 3068, 4682
VpaK11BI GGWCC 1 cut(s) 2146
VspI ATTAAT 2 cut(s) 474, 4028
XapI RAATTY 8 cut(s) 1013, 1362, 2180, 2839, 2985, 3199, 4302, 5098
XbaI TCTAGA 1 cut(s) 1312
XceI RCATGY 5 cut(s) 1326, 1879, 3343, 3878, 4456
XcmI CCANNNNNNNNNTGG 2 cut(s) 583, 862
XhoI CTCGAG 1 cut(s) 2174
XmiI GTMKAC 3 cut(s) 3931, 4251, 4443
XmnI GAANNNNTTC 3 cut(s) 598, 810, 3534
ZrmI AGTACT 3 cut(s) 1308, 1407, 2895
Zsp2I ATGCAT 2 cut(s) 1464, 4080
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.