Rw1G035880

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0010532
Physical Location & Seq
Forward (+)
63696175 .. 63718878
22704 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G035880.1

Sequence Viewer

Length: 5259 bp
ATGGCTGCTGCTGCTGATCCTTCTCCTCCTCCTCCTCCTCCTCCTCCTCACAATGATGGCCGTTATGATGTCTTCTTGAGTTTTAGAGGGAAAGACACTCGAAGGACCTTTACTCCCCATCTTTACCATGCTCTAAAACGGAAAGGACTCCATGTCTTCATAGATAACGAACAGCTTGCGAAAGGCGACGTTCTCGGGGTGCTACTGAAATCCATTGAGGCGTCAAAGCTTTCGGTGGTAGTTTTATCCGAGAACTTTGCCAATTCCAAATGGTGCTTAATGGAACTCCAAAAAATCATGGAGTGCAGGGAAACAAAGAAGCAAATAGTGATTCCCATTTTCTATCAAGTACAGGTGGCTGATGTTCGTCATGTCAGGGGACGTTTTAAGGAAGCGTTTGAAGATCATGAGCACAAGTGCACGGGCACCATTGAAGAACGGAAGCGGTGGAGGTCTGCTCTAACTAGAGCCAGCGATATTCATGGCTGGAATTCCAGCAACTCCAAGAATGACTTCGATCTTATTGATGATGTTGTTGAAGATATTTTGAAAAAGTTGAAAGACATCAGGGAAAGTAAATCCAATAATGACTTGGTTGGAATGGATTCCCACATTACAGAAATGAATTCAAGATTATGTATTCAGGTGAATGATGTTCGTGTTGTTGGAATATGGGGTATGGGTGGTCTAGGGAAGACAACCATCGCTAGAGCTGTTTATGAGGAAATGTGTGGAGACTTTGAGCACCATTGCTTTCTTTATAATGTCAAGGAAGATTTCAAGAGAAAAGGTGAAGCAGGAATGCAGGAAGAACTTCTATATAGAATCACAAGGGAAAATGGGCAGCACTTAAGTTCCAAAATGATTATGGAAAGGCTTAGTGGGATAAAACTTTTTCTTGTTCTTGATGATGTGGACAGTTCTGATCAAATTGATGCCCTACTTGGAAAAGAAATTTCATTTCTTCGTGGAAGTAAAATCATTATAACGACCAGAGATAAGGGCCTGCTTAGAGACTTTGAAGTATACAAACCGGCATATTTAGATAACGATCAAGCTCTTGAACTCTTTAAGAAGTATGCCTTCAGAAAAGTTGAATGCTCGACAGAGTACAATCATCTCTCACAGCGTGCCATAAAATATGCTCGGGGTTTGCCTTTAGCACTTAAAGTCTTGGGAGCCTCTCTCTATAAGAGAAGTGTGCCTGAGTGGGAAGTTGAATTTGAAAAAATAAAGAAATGCCAGCACTTGGGAATTGAGGGTGTACTAAGGACAACCTTTGATGGACTAGATCATGTACAGAAAACAGTGCTTCTAGATATTGCATGTTTCTTTAGAGGAATGAAAAAGGACTATGCAACAAAAATTATGGAGGCTTGTGGCTTCTTTCCCGCAAGTGTATTACCAGTTCTAGCTGATAGAGCTCTTGTGACTAGCACTGGATACTATGATGTGCTGACGATGCATGATTTACTATTGGACATGGCTTTGGGAATTGTCCGTCGAGAATCAAGCCTCAGTAGGTTGTCGAGTTCTGATGAAATCGAGGAGGTGCTAACTCAAAATACGGATTCAAAAGCAGTTGAAAGTATAGACCTCGACTTGTACCACTCAGAAGGCATACGCATAAATGCTAAAGCTTTTGTTCACATGACGAAACTAAGACTGCTCAGAATCCGATATAATCGTTCCATAGACTTTGTTGATGATTATTATGGTTCACGGGGGGAATTATACCATCTAATTAATGACTGTAAACAACAAATGAGTGGGGAGTTAAAGTTTCTATCTCATGAGTTGAGGTGTCTCATCTGGCATGGGTGCCCCCTGAAATCTTTGCCATCCAATTTCAACCCAAAGCATCTAGTCGACCTTGACATGCGTTCTAGCCGCATCAAACAACTATGGAAAGGAACCAAGCTTCTGAAAAAGTTGAAATACATTGATTTAAGTCTATCTATATGCCTTAAGGAGACGCCCAACTTAACCGAGGCCACAAATCTTGAGAGAGTGAACCTTGAAGGTTGTACAAGTTTATTGCAGGTTCACCCAACCATCTCTGATCTTAAGAACCTCCTTTTCTTGAGTCTAAAAGGGTGCAAAGAGCTTAAGATTCTTCCAAGCAGCTTCCGTATGAAATCTCTTGAGACCCTTGAGCTTTCTGGCTGCTCAAACCTCGAGAAATTTCCGGAGATTTCAGAAGTTATGGCGGAGCTGCCACGGCTCTGTTTGGATAAGACTGCAATTAAAGAACTGCCCTCATCAATAAAAAATCTTAGGGGGCTTGTCACCTTGAGCCTAAGAGATTGCAGAGAACTTGAGAGCCTCCCAAGCAGCATTTGTCTACTCAAGTCCCTTACATATGTTACTCTCTCTGGGTGTTCTAAGTTTGAGGTGTTTCCCAAAATTATGGGGGTAGATGATATGGAAAAATTAAAAGAGCTTCATTTGGATGGAACATCTATCAAAGAGTTTTTGCCGTCTACTTCAGCTCTTAAAATCCTTGAAGTCTTGAGTCTAAGAGAATGCAAAGAGCTTAAGACTCTTCCAAGTAGCATTTGTATGAGATCTCTTAACACGCTTATTCTTTCTGGATGTTCAAACCTTAAGAAGTTTCCAGAGATTTCAGAAAAAATGTTTAGGCTCACACAGCTTCATTTAGATGATATTGCAATTGAAGAACTGCCACTGTCCATTAAAAATCTTTGCCAGATCGAATCACTGAGCCTTAAAGATTGTGGAGAACTTAAGAGTCTTCCGAGCAGCATTTTTCAACTTGAGTACCTCAAAGTGATTACTGTCTCTGGATGTTCGAAGTTTGAGGTGTTTCCGAAAATTGAAGATTTGGAAGGACTAAGAAAACTTCTTACATTGAGGCTAGAAGATTGTGGAGAACTTAGGACTCTGCCCAGCAGCATTTGTCAACTCAAGTCCCTTAGATTTGTTTCTCTCTCTGGTTGTTCGAAGTTTGAGGTGTTTCCGGAAATTATAGAAGATATGGAAGAAGTAAGAGAGCTTCATCTTGATGGGACGTCTATTGAAGCACTTTCCCCATCAATTTATCAGCTGAAGAGGCTTATGTTACTGAACATGAGAAACTGCAAGAGCCTTCAATTACTTCCAGACAGTGTCTGTAATTTGTCACACCTTACAGGTCTCTCTCTCTCAGGGTGCTCTAATGTCCATACCTTGCCCGAAAACATGGGAATGTTAAAATACTTGGTGGACCTTGAAATACAGGGAAGTGGTATAAAGCAACCATTCTCTCCCCCATCATCATCAACCTATTACTCTGTTGGAATGGATCGCCACATAGATGAAATGCAAAAGCTATTATGTCCTGATCAGGTGGATGATGTTCGTATTGTTGGAATATGTGGAATTGGTGGCATCGGCAAGACAACCATTGCTACAGCTGTTTATGATAAAATTGCTCCTCAATTTGAACACCATTGTTTTCTTGAGAATGTCAAGGAAGGTTTCACAAAGCATGGTGCAGAACAGATGCAGGAAGAACTTCTGACTAGAATCTTAAAGGTTAAGGTGCAGAGTTTAGGCATTTTGAATAGAGGCTCCAATATGCTTATCGAAAGGCTAGGTATGAAAAAGGTTTTAGTTGTTCTTGATGATATAGAAGAGAAAACCCAGATTAACACTTTGCTTGAAGACCCATATTCATTTGGTGGTGGGAGTAGGATCATTGTAACAACTAGAAAGGAAGAAATAATGAGTGGATTTAAAATATATCGTCCCCAGCTATTAAATGCTGAAGCTGTTGAAATCTTCAAACATTTTGCCTTCAGAACAATTGAACCACCCTCGAGAGGGTATGATGATCTCTCACAGCGTGCCACACAACATGCTCAAGTGCCTTTAGATCTTAAAGTCTGGGGAGCTTTTCTTTTCAACAAAAATATAGATGAGTGGAAAGATGCCTTGACGGATAAGCACACGGTTTATTGGGTGCTAACAAAATTCTTTGATGGCCTACATTACTCAGAGAGGAACATATTAATAGATGTTGCATGGTTCTTTGAAGGAATGAAAAAAGAGTATGCAACAAAAATTCTGCACAGTTCTGGCCTCTTTTCCTATAGTGCATTAAGAGGTCTAATTGATAGATCTCTCGTATCTATCTCAGAAAGGGACGGGACTATCAAAATTCATGATTTACTACATAGGATTGTTCGGGATTGCGTTCGCCACGAAGATGAACATGAACCTCGTAACCGCAGCAGATTAGAGGGTACCCAGGCAATTAGTTGTTTGTCAACTCAAGATCAGGTTAATAAAGCAGTTGAAGTTATGACCCTTGATCTGTTTGACAGAAAAGAGGTATACTTCAGTGCAGAGGCTTTTGTTACAATGAAGAAATTAAGACTGCTCCGAATTTATGATAACGGCTCCATTAATTGTAAACAACATGTGATTGGAGACTTTGAGTTTATTTCTCATGATTTGGGGTGTCTCATCTGGCATAGATACCCCCTAAAGTCTTTGCCATCTAATTTTCACCCAAAGAATCTTGTCGACCTTGACATGCGTTTTAGTCGCCTTGAACAACTTTGGGAAGGAACCAAGCCTCTGAAGTACTTGACATTCATGAATTTAAGTCACTCTCAATATCTTGAGAAAATCCCCGACTTGACTGAGGCTACAAATCTTGAGAAGCTAATTCTTGATGGTTGTACAAGTTTATGTGAGGTGCACCCATCCATTTGTCATCATAAAAACCTCGTTTTCTTGAGTCTAAAGGGCTGCATAGAACTTAAGATTCTTCCAACCAGCATCCATATGAATTCTCTTAAGACCCTCAACCTTTCTGGCTGCTTAAATCTTGAGAAGTTTCCTGACATTTCAGAAGTTATGATGGAGCTAGCAGAGCTTGGCTTAGATGAGACTGCAATTAACGAACTGCCCTCATCAATTCAACGTCTTCAGGGACTTAATGTATTAAGTATGAGAAACTGCGCTAGCCTTATCTCTCTTCCGGACAGCATCTGTAATTTGGAACACCTTACATTTCTCAATCTATCTGGGTGCTCAAAACTGTCCGACTTTCCTGACAGCTTGAGGAATACACAATCATTGACCATCAGTGGTATAGAACAACTCCCACTCCTGATGAGAAGCAAAGAAACAGAGATGGAGGGAGTCATCGGAGTCTCCGGGCAGGGGAGTTCATCAAAGTCAGGGAAAGGAAGAAAGTTGCCTGTGCCAAAGCCACAGAGAGTGAGAGTGAAGAAGAGAAAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

1752

Amino Acids

199.01

Weight (kDa)

7.26

Isoelectric Point (pI)

39.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 22 - 200 4.4e-50 TIR domain
TIR_2 PF13676 24 - 117 8.4e-15 TIR domain
NB-ARC PF00931 204 - 364 3.8e-17 NB-ARC domain
WHD_ROQ1 PF23282 434 - 504 5.3e-13 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 704 - 838 2e-11 Leucine-rich repeat region
LRR_13 PF23286 713 - 797 6.4e-14 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_13 PF23286 856 - 940 2.1e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 858 - 960 4.4e-08 Leucine-rich repeat region
LRR_14 PF23598 958 - 1081 5.5e-11 Leucine-rich repeat region
LRR_13 PF23286 976 - 1062 9.7e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
NB-ARC PF00931 1103 - 1268 3.3e-16 NB-ARC domain
WHD_ROQ1 PF23282 1333 - 1402 3.2e-11 Disease resistance protein Roq1-like, winged-helix domain
LRR_3 PF07725 1508 - 1527 1.7e-06 Leucine Rich Repeat
LRR_14 PF23598 1598 - 1697 8.1e-11 Leucine-rich repeat region
LRR_13 PF23286 1599 - 1686 7e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 762, 986
AatII GACGTC 1 cut(s) 3035
Acc36I ACCTGC 1 cut(s) 2032
Acc65I GGTACC 1 cut(s) 4247
AccB1I GGYRCC 3 cut(s) 425, 1821, 4247
AccB7I CCANNNNNTGG 1 cut(s) 1249
AccI GTMKAC 6 cut(s) 1026, 1869, 2344, 2483, 4338, 4530
AccIII TCCGGA 3 cut(s) 2188, 2980, 4990
AciI CCGC 5 cut(s) 445, 1392, 1891, 2210, 4231
AclWI GGATC 3 cut(s) 11, 3312, 3704
AcoI YGGCCR 1 cut(s) 58
AcuI CTGAAG 8 cut(s) 1069, 2472, 3089, 3784, 3789, 4327, 4607, 4922
AcyI GRCGYC 3 cut(s) 221, 1976, 3032
AdeI CACNNNGTG 2 cut(s) 1130, 3848
AfiI CCNNNNNNNGG 4 cut(s) 1249, 3824, 3825, 5175
AflII CTTAAG 9 cut(s) 850, 1967, 2066, 2108, 2537, 2606, 2747, 4769, 4805
AflIII ACRYGT 1 cut(s) 4423
AhdI GACNNNNNGTC 1 cut(s) 152
AjnI CCWGG 1 cut(s) 4251
AjuI GAANNNNNNNTTGG 2 cut(s) 497, 529
Alw21I GWGCWC 7 cut(s) 414, 422, 747, 1426, 3176, 4710, 5045
Alw44I GTGCAC 2 cut(s) 418, 4706
AlwI GGATC 3 cut(s) 11, 3312, 3704
AlwNI CAGNNNCTG 2 cut(s) 3132, 5001
Ama87I CYCGRG 4 cut(s) 194, 1146, 2177, 3820
Aor13HI TCCGGA 3 cut(s) 2188, 2980, 4990
AoxI GGCC 5 cut(s) 58, 1003, 1992, 3985, 4081
ApaLI GTGCAC 2 cut(s) 418, 4706
AseI ATTAAT 3 cut(s) 1746, 4013, 4410
Asp700I GAANNNNTTC 4 cut(s) 512, 604, 813, 3516
Asp718I GGTACC 1 cut(s) 4247
AspLEI GCGC 1 cut(s) 4973
AspS9I GGNCC 3 cut(s) 105, 1003, 3226
AsuC2I CCSGG 1 cut(s) 5170
AsuHPI GGTGA 5 cut(s) 658, 803, 2039, 2281, 4505
AsuII TTCGAA 2 cut(s) 2813, 2963
AsuNHI GCTAGC 2 cut(s) 4876, 4973
AvaI CYCGRG 4 cut(s) 194, 1146, 2177, 3820
AvaII GGWCC 2 cut(s) 105, 3226
BaeGI GKGCMC 4 cut(s) 422, 428, 1826, 4710
BaeI ACNNNNGTAYC 2 cut(s) 2765, 2798
BanI GGYRCC 3 cut(s) 425, 1821, 4247
BanII GRGCYC 1 cut(s) 1426
BarI GAAGNNNNNNTAC 4 cut(s) 94, 126, 3000, 3032
BbsI GAAGAC 6 cut(s) 64, 148, 701, 2747, 3672, 4928
Bbv12I GWGCWC 7 cut(s) 414, 422, 747, 1426, 3176, 4710, 5045
BceAI ACGGC 4 cut(s) 45, 2237, 2464, 4417
BcgI CGANNNNNNTGC 4 cut(s) 158, 192, 239, 273
BciT130I CCWGG 1 cut(s) 4253
BciVI GTATCC 1 cut(s) 1436
BclI TGATCA 2 cut(s) 925, 3343
BcnI CCSGG 1 cut(s) 5170
BfmI CTRYAG 2 cut(s) 3411, 4093
BfrI CTTAAG 9 cut(s) 850, 1967, 2066, 2108, 2537, 2606, 2747, 4769, 4805
BfuAI ACCTGC 1 cut(s) 2032
BfuI GTATCC 1 cut(s) 1436
BglII AGATCT 3 cut(s) 2567, 3877, 4121
BmcAI AGTACT 1 cut(s) 4592
Bme1390I CCNGG 2 cut(s) 4253, 5170
Bme18I GGWCC 2 cut(s) 105, 3226
BmeRI GACNNNNNGTC 1 cut(s) 152
BmeT110I CYCGRG 4 cut(s) 194, 1146, 2177, 3820
BmgT120I GGNCC 3 cut(s) 105, 1003, 3226
BmiI GGNNCC 8 cut(s) 427, 1180, 1823, 1915, 3574, 4249, 4405, 4576
BmrFI CCNGG 2 cut(s) 4253, 5170
BmsI GCATC 9 cut(s) 925, 1452, 1870, 1902, 3399, 3495, 3922, 4797, 5007
BmtI GCTAGC 2 cut(s) 4880, 4977
BpiI GAAGAC 6 cut(s) 64, 148, 701, 2747, 3672, 4928
BplI GAGNNNNNCTC 4 cut(s) 442, 474, 1170, 1202
Bpu14I TTCGAA 2 cut(s) 2813, 2963
BpuMI CCSGG 1 cut(s) 5170
BsaBI GATNNNNATC 1 cut(s) 1050
BsaHI GRCGYC 3 cut(s) 221, 1976, 3032
BsaI GGTCTC 2 cut(s) 2141, 3161
BsaJI CCNNGG 3 cut(s) 1989, 2219, 4251
BsaWI WCCGGW 3 cut(s) 2188, 2980, 4990
BsaXI ACNNNNNCTCC 6 cut(s) 97, 127, 1766, 1796, 5103, 5133
Bsc4I CCNNNNNNNGG 4 cut(s) 1249, 3824, 3825, 5175
Bse118I RCCGGY 1 cut(s) 1033
Bse1I ACTGG 2 cut(s) 1406, 1444
Bse3DI GCAATG 2 cut(s) 748, 3405
Bse8I GATNNNNATC 1 cut(s) 1050
BseAI TCCGGA 3 cut(s) 2188, 2980, 4990
BseBI CCWGG 1 cut(s) 4253
BseDI CCNNGG 3 cut(s) 1989, 2219, 4251
BseGI GGATG 7 cut(s) 1841, 2458, 2600, 2813, 3358, 4712, 4788
BseJI GATNNNNATC 1 cut(s) 1050
BseLI CCNNNNNNNGG 4 cut(s) 1249, 3824, 3825, 5175
BseMI GCAATG 2 cut(s) 748, 3405
BseMII CTCAG 9 cut(s) 1197, 1531, 1626, 1684, 2714, 3180, 4011, 4152, 4641
BseNI ACTGG 2 cut(s) 1406, 1444
BseSI GKGCMC 4 cut(s) 422, 428, 1826, 4710
BseYI CCCAGC 2 cut(s) 2909, 3753
BsgI GTGCAG 5 cut(s) 325, 3516, 3566, 4055, 4368
BshFI GGCC 5 cut(s) 60, 1005, 1994, 3987, 4083
BshNI GGYRCC 3 cut(s) 425, 1821, 4247
BsiHKAI GWGCWC 7 cut(s) 414, 422, 747, 1426, 3176, 4710, 5045
BsiHKCI CYCGRG 4 cut(s) 194, 1146, 2177, 3820
BsiSI CCGG 5 cut(s) 1034, 2189, 2981, 4991, 5169
BslFI GGGAC 8 cut(s) 393, 2338, 2917, 3043, 3735, 4160, 4165, 4956
BslI CCNNNNNNNGG 4 cut(s) 1249, 3824, 3825, 5175
BsmBI CGTCTC 1 cut(s) 1967
BsmFI GGGAC 8 cut(s) 393, 2338, 2917, 3043, 3735, 4160, 4165, 4956
BsmI GAATGC 3 cut(s) 807, 1103, 2531
BsnI GGCC 5 cut(s) 60, 1005, 1994, 3987, 4083
Bso31I GGTCTC 2 cut(s) 2141, 3161
BsoBI CYCGRG 4 cut(s) 194, 1146, 2177, 3820
Bsp119I TTCGAA 2 cut(s) 2813, 2963
Bsp1286I GDGCHC 9 cut(s) 414, 422, 428, 747, 1426, 1826, 3176, 4710, 5045
Bsp13I TCCGGA 3 cut(s) 2188, 2980, 4990
Bsp1407I TGTACA 3 cut(s) 1297, 2027, 4688
BspACI CCGC 5 cut(s) 445, 1392, 1891, 2210, 4231
BspANI GGCC 5 cut(s) 60, 1005, 1994, 3987, 4083
BspCNI CTCAG 9 cut(s) 1198, 1530, 1625, 1683, 2715, 3179, 4010, 4151, 4642
BspEI TCCGGA 3 cut(s) 2188, 2980, 4990
BspHI TCATGA 5 cut(s) 406, 1792, 4165, 4453, 4602
BspLI GGNNCC 8 cut(s) 427, 1180, 1823, 1915, 3574, 4249, 4405, 4576
BspMI ACCTGC 1 cut(s) 2032
BspOI GCTAGC 2 cut(s) 4880, 4977
BspPI GGATC 3 cut(s) 11, 3312, 3704
BspT104I TTCGAA 2 cut(s) 2813, 2963
BspT107I GGYRCC 3 cut(s) 425, 1821, 4247
BspTI CTTAAG 9 cut(s) 850, 1967, 2066, 2108, 2537, 2606, 2747, 4769, 4805
BspTNI GGTCTC 2 cut(s) 2141, 3161
BsrDI GCAATG 2 cut(s) 748, 3405
BsrFI RCCGGY 1 cut(s) 1033
BsrGI TGTACA 3 cut(s) 1297, 2027, 4688
BsrI ACTGG 2 cut(s) 1406, 1444
BssAI RCCGGY 1 cut(s) 1033
BssECI CCNNGG 3 cut(s) 1989, 2219, 4251
BssNAI GTATAC 2 cut(s) 1027, 4339
BssNI GRCGYC 3 cut(s) 221, 1976, 3032
Bst1107I GTATAC 2 cut(s) 1027, 4339
Bst2UI CCWGG 1 cut(s) 4253
Bst4CI ACNGT 9 cut(s) 920, 1309, 1754, 2691, 2800, 3128, 3955, 4076, 5052
Bst6I CTCTTC 5 cut(s) 2550, 3065, 3630, 4992, 5240
BstACI GRCGYC 3 cut(s) 221, 1976, 3032
BstAFI CTTAAG 9 cut(s) 850, 1967, 2066, 2108, 2537, 2606, 2747, 4769, 4805
BstAUI TGTACA 3 cut(s) 1297, 2027, 4688
BstBI TTCGAA 2 cut(s) 2813, 2963
BstC8I GCNNGC 8 cut(s) 177, 472, 1007, 1131, 1244, 3849, 4878, 4975
BstDSI CCRYGG 1 cut(s) 2219
BstF5I GGATG 7 cut(s) 1841, 2458, 2600, 2813, 3358, 4712, 4788
BstHHI GCGC 1 cut(s) 4973
BstMWI GCNNNNNNNGC 8 cut(s) 11, 1421, 1462, 2221, 2331, 2650, 3073, 4882
BstNI CCWGG 1 cut(s) 4253
BstNSI RCATGY 5 cut(s) 1329, 1882, 3863, 4427, 4543
BstSCI CCNGG 2 cut(s) 4251, 5168
BstSFI CTRYAG 2 cut(s) 3411, 4093
BstSLI GKGCMC 4 cut(s) 422, 428, 1826, 4710
BstV2I GAAGAC 6 cut(s) 64, 148, 701, 2747, 3672, 4928
BstX2I RGATCY 3 cut(s) 2567, 3877, 4121
BstXI CCANNNNNNTGG 1 cut(s) 2410
BstYI RGATCY 3 cut(s) 2567, 3877, 4121
BstZ17I GTATAC 2 cut(s) 1027, 4339
BsuI GTATCC 1 cut(s) 1436
BsuRI GGCC 5 cut(s) 60, 1005, 1994, 3987, 4083
BtgI CCRYGG 1 cut(s) 2219
BtgZI GCGATG 1 cut(s) 688
BtsCI GGATG 7 cut(s) 1841, 2458, 2600, 2813, 3358, 4712, 4788
BtsIMutI CAGTG 7 cut(s) 1314, 1437, 2687, 2720, 3133, 4351, 5104
BveI ACCTGC 1 cut(s) 2032
Cac8I GCNNGC 8 cut(s) 177, 472, 1007, 1131, 1244, 3849, 4878, 4975
CaiI CAGNNNCTG 2 cut(s) 3132, 5001
CciI TCATGA 5 cut(s) 406, 1792, 4165, 4453, 4602
CfoI GCGC 1 cut(s) 4973
Cfr10I RCCGGY 1 cut(s) 1033
Cfr13I GGNCC 3 cut(s) 105, 1003, 3226
CseI GACGC 2 cut(s) 210, 1984
CspCI CAANNNNNGTGG 2 cut(s) 1984, 2019
DraI TTTAAA 1 cut(s) 3739
DraIII CACNNNGTG 2 cut(s) 1130, 3848
DriI GACNNNNNGTC 1 cut(s) 152
EaeI YGGCCR 1 cut(s) 58
Eam1104I CTCTTC 5 cut(s) 2550, 3065, 3630, 4992, 5240
Eam1105I GACNNNNNGTC 1 cut(s) 152
EarI CTCTTC 5 cut(s) 2550, 3065, 3630, 4992, 5240
EciI GGCGGA 1 cut(s) 2225
Ecl136II GAGCTC 1 cut(s) 1424
Eco24I GRGCYC 1 cut(s) 1426
Eco31I GGTCTC 2 cut(s) 2141, 3161
Eco47I GGWCC 2 cut(s) 105, 3226
Eco53kI GAGCTC 1 cut(s) 1424
Eco57I CTGAAG 8 cut(s) 1069, 2472, 3089, 3784, 3789, 4327, 4607, 4922
Eco88I CYCGRG 4 cut(s) 194, 1146, 2177, 3820
EcoICRI GAGCTC 1 cut(s) 1424
EcoO109I RGGNCCY 2 cut(s) 105, 1003
EcoRI GAATTC 3 cut(s) 490, 625, 4798
EcoRII CCWGG 1 cut(s) 4251
EcoT22I ATGCAT 1 cut(s) 1467
EcoT38I GRGCYC 1 cut(s) 1426
Esp3I CGTCTC 1 cut(s) 1967
FalI AAGNNNNNCTT 4 cut(s) 497, 529, 1067, 1099
FaqI GGGAC 8 cut(s) 393, 2338, 2917, 3043, 3735, 4160, 4165, 4956
FauI CCCGC 1 cut(s) 1399
FauNDI CATATG 2 cut(s) 2362, 4794
FbaI TGATCA 2 cut(s) 925, 3343
FblI GTMKAC 6 cut(s) 1026, 1869, 2344, 2483, 4338, 4530
FokI GGATG 7 cut(s) 1828, 2465, 2607, 2820, 3365, 4699, 4775
FriOI GRGCYC 1 cut(s) 1426
GlaI GCGC 1 cut(s) 4972
GsaI CCCAGC 2 cut(s) 2913, 3757
HaeIII GGCC 5 cut(s) 60, 1005, 1994, 3987, 4083
HapII CCGG 5 cut(s) 1034, 2189, 2981, 4991, 5169
HgaI GACGC 2 cut(s) 210, 1984
HhaI GCGC 1 cut(s) 4973
Hin1I GRCGYC 3 cut(s) 221, 1976, 3032
Hin6I GCGC 1 cut(s) 4971
HinP1I GCGC 1 cut(s) 4971
HincII GTYRAC 4 cut(s) 1870, 2924, 4272, 4531
HindII GTYRAC 4 cut(s) 1870, 2924, 4272, 4531
HindIII AAGCTT 3 cut(s) 227, 1638, 1919
HpaII CCGG 5 cut(s) 1034, 2189, 2981, 4991, 5169
HphI GGTGA 5 cut(s) 658, 803, 2039, 2281, 4505
Hpy99I CGWCG 2 cut(s) 191, 1506
HpyCH4III ACNGT 9 cut(s) 920, 1309, 1754, 2691, 2800, 3128, 3955, 4076, 5052
HpyCH4IV ACGT 4 cut(s) 189, 382, 3032, 4933
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 1421, 1462, 2221, 2331, 2650, 3073, 4882
HpySE526I ACGT 4 cut(s) 189, 382, 3032, 4933
Hsp92I GRCGYC 3 cut(s) 221, 1976, 3032
HspAI GCGC 1 cut(s) 4971
Kpn2I TCCGGA 3 cut(s) 2188, 2980, 4990
KpnI GGTACC 1 cut(s) 4251
Ksp22I TGATCA 2 cut(s) 925, 3343
LmnI GCTCC 8 cut(s) 1178, 2212, 3439, 3578, 3893, 4389, 4409, 4873
LweI GCATC 9 cut(s) 925, 1452, 1870, 1902, 3399, 3495, 3922, 4797, 5007
MaeII ACGT 4 cut(s) 189, 382, 3032, 4933
MaeIII GTNAC 9 cut(s) 1429, 2287, 2365, 3081, 3141, 3703, 4226, 4360, 4613
MfeI CAATTG 2 cut(s) 2673, 3807
MflI RGATCY 3 cut(s) 2567, 3877, 4121
MhlI GDGCHC 9 cut(s) 414, 422, 428, 747, 1426, 1826, 3176, 4710, 5045
MlyI GAGTC 9 cut(s) 141, 2095, 2524, 2536, 2761, 2896, 4756, 5163, 5172
MmeI TCCRAC 6 cut(s) 577, 646, 3277, 3349, 4805, 5079
Mph1103I ATGCAT 1 cut(s) 1467
MroI TCCGGA 3 cut(s) 2188, 2980, 4990
MroXI GAANNNNTTC 4 cut(s) 512, 604, 813, 3516
MslI CAYNNNNRTG 9 cut(s) 54, 2451, 2561, 2661, 3024, 3206, 3315, 4209, 4793
MspA1I CMGCKG 2 cut(s) 3067, 3416
MspCI CTTAAG 9 cut(s) 850, 1967, 2066, 2108, 2537, 2606, 2747, 4769, 4805
MspI CCGG 5 cut(s) 1034, 2189, 2981, 4991, 5169
MspR9I CCNGG 2 cut(s) 4253, 5170
MunI CAATTG 2 cut(s) 2673, 3807
Mva1269I GAATGC 3 cut(s) 807, 1103, 2531
MvaI CCWGG 1 cut(s) 4253
MwoI GCNNNNNNNGC 8 cut(s) 11, 1421, 1462, 2221, 2331, 2650, 3073, 4882
NciI CCSGG 1 cut(s) 5170
NdeI CATATG 2 cut(s) 2362, 4794
NheI GCTAGC 2 cut(s) 4876, 4973
NlaIV GGNNCC 8 cut(s) 427, 1180, 1823, 1915, 3574, 4249, 4405, 4576
NmuCI GTSAC 4 cut(s) 1429, 2287, 3141, 4613
NsiI ATGCAT 1 cut(s) 1467
NspI RCATGY 5 cut(s) 1329, 1882, 3863, 4427, 4543
NspV TTCGAA 2 cut(s) 2813, 2963
PaeR7I CTCGAG 2 cut(s) 2177, 3820
PagI TCATGA 5 cut(s) 406, 1792, 4165, 4453, 4602
PciI ACATGT 1 cut(s) 4423
PctI GAATGC 3 cut(s) 807, 1103, 2531
PdmI GAANNNNTTC 4 cut(s) 512, 604, 813, 3516
PflFI GACNNNGTC 1 cut(s) 3128
PflMI CCANNNNNTGG 1 cut(s) 1249
PleI GAGTC 9 cut(s) 141, 2094, 2523, 2536, 2760, 2896, 4755, 5162, 5171
PpsI GAGTC 9 cut(s) 141, 2094, 2523, 2536, 2760, 2896, 4755, 5162, 5171
PpuMI RGGWCCY 1 cut(s) 105
PscI ACATGT 1 cut(s) 4423
PshBI ATTAAT 3 cut(s) 1746, 4013, 4410
PsiI TTATAA 2 cut(s) 762, 986
Psp124BI GAGCTC 1 cut(s) 1426
Psp5II RGGWCCY 1 cut(s) 105
Psp6I CCWGG 1 cut(s) 4251
PspFI CCCAGC 2 cut(s) 2909, 3753
PspGI CCWGG 1 cut(s) 4251
PspN4I GGNNCC 8 cut(s) 427, 1180, 1823, 1915, 3574, 4249, 4405, 4576
PspPI GGNCC 3 cut(s) 105, 1003, 3226
PspPPI RGGWCCY 1 cut(s) 105
PsrI GAACNNNNNNTAC 2 cut(s) 1392, 1424
PstNI CAGNNNCTG 2 cut(s) 3132, 5001
PsuI RGATCY 3 cut(s) 2567, 3877, 4121
PsyI GACNNNGTC 1 cut(s) 3128
PvuII CAGCTG 2 cut(s) 3067, 3416
RseI CAYNNNNRTG 9 cut(s) 54, 2451, 2561, 2661, 3024, 3206, 3315, 4209, 4793
SacI GAGCTC 1 cut(s) 1426
SalI GTCGAC 2 cut(s) 1868, 4529
Sau96I GGNCC 3 cut(s) 105, 1003, 3226
ScaI AGTACT 1 cut(s) 4592
SchI GAGTC 9 cut(s) 141, 2095, 2524, 2536, 2761, 2896, 4756, 5163, 5172
ScrFI CCNGG 2 cut(s) 4253, 5170
SduI GDGCHC 9 cut(s) 414, 422, 428, 747, 1426, 1826, 3176, 4710, 5045
SfaNI GCATC 9 cut(s) 925, 1452, 1870, 1902, 3399, 3495, 3922, 4797, 5007
SfcI CTRYAG 2 cut(s) 3411, 4093
Sfr274I CTCGAG 2 cut(s) 2177, 3820
SfuI TTCGAA 2 cut(s) 2813, 2963
SinI GGWCC 2 cut(s) 105, 3226
SlaI CTCGAG 2 cut(s) 2177, 3820
SmiMI CAYNNNNRTG 9 cut(s) 54, 2451, 2561, 2661, 3024, 3206, 3315, 4209, 4793
SsiI CCGC 5 cut(s) 445, 1392, 1891, 2210, 4231
SstI GAGCTC 1 cut(s) 1426
StyD4I CCNGG 2 cut(s) 4251, 5168
TaaI ACNGT 9 cut(s) 920, 1309, 1754, 2691, 2800, 3128, 3955, 4076, 5052
TaiI ACGT 4 cut(s) 192, 385, 3035, 4936
TatI WGTACW 7 cut(s) 349, 1110, 1264, 1297, 2027, 4590, 4688
TauI GCSGC 1 cut(s) 1893
TscAI CASTG 7 cut(s) 1314, 1444, 2694, 2727, 3133, 4351, 5104
TseFI GTSAC 4 cut(s) 1429, 2287, 3141, 4613
Tsp45I GTSAC 4 cut(s) 1429, 2287, 3141, 4613
TspGWI ACGGA 6 cut(s) 154, 454, 1490, 1583, 2120, 3956
TspRI CASTG 7 cut(s) 1314, 1444, 2694, 2727, 3133, 4351, 5104
Tth111I GACNNNGTC 1 cut(s) 3128
Van91I CCANNNNNTGG 1 cut(s) 1249
Vha464I CTTAAG 9 cut(s) 850, 1967, 2066, 2108, 2537, 2606, 2747, 4769, 4805
VneI GTGCAC 2 cut(s) 418, 4706
VpaK11BI GGWCC 2 cut(s) 105, 3226
VspI ATTAAT 3 cut(s) 1746, 4013, 4410
XbaI TCTAGA 1 cut(s) 1315
XceI RCATGY 5 cut(s) 1329, 1882, 3863, 4427, 4543
XcmI CCANNNNNNNNNTGG 2 cut(s) 589, 865
XhoI CTCGAG 2 cut(s) 2177, 3820
XmiI GTMKAC 6 cut(s) 1026, 1869, 2344, 2483, 4338, 4530
XmnI GAANNNNTTC 4 cut(s) 512, 604, 813, 3516
ZraI GACGTC 1 cut(s) 3033
ZrmI AGTACT 1 cut(s) 4592
Zsp2I ATGCAT 1 cut(s) 1467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.