Rroxscaffold_4G00282460

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0010532
Physical Location & Seq
Reverse (-)
4511646 .. 4532903
21258 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00282460.1

Sequence Viewer

Length: 3387 bp
ATGCGTTCTAGCCGCATCAGACAACTTTGGAAAGGAACCAAGCTTCTGAAAAAGTTGAAATACATTGATTTAAGTCTATCTCTATACCTTAAGGAGACGCCCGACTTAACTGCGGCCACAAATCTTGAGAAAGTAAACCTTGAAGGTTGTACAAGTTTATTGGAGGTTCACCCATCCATCTCTGATCTTAAGAACCTCGCTTTCTTGAGTCTAAAAGGGTGCAAAGAGCTTAAGATTCTTCCAAGCAGTTTCTGTATGAAATCTCTTAAGACCCTTGAGCTTTCTGGCTGCTCAAATCTCGACAAATTTCCTGAGATTTCAGAAGTTATGGCCGAGCTACCACGGCTTTCTTTAGATAAGACTGCAATTAAAGAACTGCCCTCATCAATAAAAAATCTTAGGGGGCTTGTCACTTTGAGCCTAAAAGATTGCAGAGAACTTGAGAGTCTCCCAAGCAGCATTTGTCAACTCAAGTCCCTTAGATATGTTACTCTCTCTGGGTGTTCTAAGTTTAAGGTGTTTCCAAAAATTGTGGGGGTAGAAGATATGAAAGGATTAATAGAGCTTCATATGGATGGAACGTCTATCGAAGAGTTTCTGCCGTCAATTTCAGCTCTTAAAGAACTTGTTGTCTTGAGTCTAAGAGATTGCAAACAGCTAAAGAGTCTTCCTAGTAGCATTCATATGAAATCTCTTGACACACTTATTCTTTCTGGATGTTTGAACCTTCAGAAGTTTCCGGAGATTTCAGAAAAAATGTGGAGGCTCTCAGAGCTTCATTTAGATGATGTTGCAATTGAAGAACTGCCGCTGTCAATTAAAAATCTTTGCCAGCTGGAATCTTTGAGCCTTAAAGATTGTGGAGAACTCAAGAGTCTTCCAAGCAGCATTTTTGAACTTGAGAGCCTCAGTATGATTACTGTCTCTGGTTGTTCAAAGTTTAAGGTGTTTCCACAAAATGAAGAAGATTTGGAAGGACTACAAAAACTTGTTACATTGAGGCTAGAAGATTGTGGAGAACTTAGGAGTCTGCCAAGCAGCATTTGTCAACTCAAGTCCCTCAGATTTGTTGCTCTCTCTGGTTGTTCAAAGTTTGAAGTGTTTCCGGAAATTTTAGAAGCTATGGAAGAATTAAGAGAGCTTCATTTGGATGGAACATCTGTCAAAGACCTTTCCCCATCAATTGAACGGCTTAAGAGGATTATGTTATTGAACATGAGAAACTGCAGAAGCCTCATCTTTCTTCCTGAAAATATCTGTAATTTGTCATACCTTACAGGTCTCACTCTCTCAGGGTGCTTGAATCTCCATAACTTGCCCAAGAACTTGGGGAAATTAGAATCCTTGGTGGATCTTGAAGTACAGGGAAGTGGTATAGAGCAACCATTCTCTCTCTTGCATCCAAAGAAGTCACCATCATCATCAGCCAATTGCTTAGTTGGCATGGATCGCCATATAAATAAAATGCAATCGCTATTATGTCTTGATCAGGTGGATGATGTTCGTATTGTTGGAATATGTGGAATTGGGGGTATCGGCAAGACAACCATTGCTACAGCTGTATATGATAAAATTGCTCCTCAATTTGAACACCATTGTTTTCTTGAGAATGTCAAGGAAGGTTTCACAAAGCATAGTGCAGAACAAATGCAGGAAGAACTTCTGACCAGAATCTTAAAGGCAAAGGTGCAGAGTTTAAGCATTTTGAACAGAGGCTCCAATATGCTTATCGAAAGGCTAGGTATGAAAAAAGTTCTAGTTGTTCTTGATGATATAGACGATATAACCCAGATTGAAACCTTGCTTGAAAAGCCATATTCATTTGGTGGTGGGAGTAGGATCATTGTCACAACTAGAAAGGAAGAAATAATGAGTGGATTTAAAATATATTGTCCCCAGCTATCAAATGCTGAAGCTGTTGAAATCTTCAGACATTTTGCCTTCAGAACAATTGAACCCTCGAGAGGGTATGATAATCTGTCACAGCGTGCTGCACAACATGCTCAAGTGCCTTTAGATCTTAAAGTCTGGGGAGCTTTTCTTTTCAACAAAAGTATACATGAGTGGGAAGATGCCTTGACGGATAAGCACACGGTGTATTGGGTGCTAACAACACACTTTTATGGCCTACATTACTCGGAGAGGAACATATTAATAGATGTTGCATGGTTCTTTGAAGGAATGAAAAAAGAGTATGCAACAAAAATTCTGCACAATTGTGGCCTCTTTCCCTATAGTGCATTAAGAGGTCTAATTGATAGATCTCTCGTATCTATATCAGAAAGGGACGGGACTATCAAACTGCATGATTTACTACAGGGGATTGTTCGGGATTGTGTTCGCCACGAAGATGAACATGAACCTGGAAACCGCAGTAGATTAGAGGGTTACCAGGGAATTAGGTGTTTGTCAACTCCAGATCAGATTACTAAAGCAGTTGAAGTTGTAACCCTAGATCTGTCGAACTCAAAAGAGGTATACTTCATTGCAGATGCTTTTGTTACAATGAAGAAATTAAGACTGCTCCGAATTTATGATAACGGTTCCATTAACTGTAAACAACATGTGATTGGAGACTTTGAGTTTATTTCTCATGAGTTGAGGTGTCTCATCTGGCATAGATACCCCGTAAAGTCTTTGCCATCCAATTTTCACCCAAAGAATCTTGTCGACCTTGACATGCGTTTTAGTCGCCTTGAACAACTTTGGGAAGGAACCAAGCTTCTGAAGTACTTGACATTCATCAATTTAAGTCACTCTCAGTATCTTAAGGAAATCCCCGACTTAACCACGGCTACAAATCTTGAGAAGCTAATTCTTGATGGTTGTACAAGTTTATTCGAGGTGCATCCATCCATTTGGAATCATAAAAACCTCATTTTCTTAAGTCTAAAAGGTTGCAGACAACTTGAGATTCTTCCAACCAACATCCATATGAAATCTCTTAAGACCCTTAATCTTTCTGGCTGCACAAATCTTGAGAAGTTTCCGGAGATTTCAGAAGTTATGATGGAGCTATCAGAGCTTGGTTTAGATGAGACTGCAATTAAAAAACTGCCCTCATCAATTGAACGGCTACAGGGGCTTAATGTATTAAGTATGAGAAACTGCACGAGCCTTGTCTCTCTTCCAGACAGCATCTGTAATTTGGCACACCTTACATTTCTCAATCTGTCTGGGTGCTCAAAACTTTCCGACTTTCCTGACAGCTTGAGGAATACACAATCATTGACAATCAGTGGTTTAGAAGAACTCACATTCTGTATGAGAAACAAAGAAATAGAAAGTGAGATTTCATCAGACGAATTCATCAGTCTCTCTGAGAATTCATCGGAGTCGGAGGAAGAGGTTGATGGAAAAGAAGAGAAAGTGGAGATGGAGGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

1128

Amino Acids

127.4

Weight (kDa)

5.99

Isoelectric Point (pI)

43.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 77 - 219 7.5e-14 Leucine-rich repeat region
LRR_13 PF23286 87 - 172 1.2e-12 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 222 - 336 6.7e-08 Leucine-rich repeat region
LRR_13 PF23286 229 - 315 1.8e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 327 - 492 1.9e-09 Leucine-rich repeat region
LRR_13 PF23286 351 - 438 4e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
NB-ARC PF00931 485 - 649 9.3e-17 NB-ARC domain
WHD_ROQ1 PF23282 713 - 782 7.4e-14 Disease resistance protein Roq1-like, winged-helix domain
LRR_3 PF07725 888 - 907 1e-06 Leucine Rich Repeat
LRR_14 PF23598 973 - 1078 2.1e-11 Leucine-rich repeat region
LRR_13 PF23286 979 - 1066 1.3e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 2056, 2478, 2670
AccIII TCCGGA 3 cut(s) 739, 1105, 2989
AciI CCGC 4 cut(s) 13, 113, 809, 2371
AclWI GGATC 3 cut(s) 1359, 1455, 1847
AcoI YGGCCR 2 cut(s) 114, 330
AcsI RAATTY 6 cut(s) 305, 1110, 2205, 2529, 3305, 3325
AcuI CTGAAG 5 cut(s) 713, 1912, 1927, 1932, 2747
AcyI GRCGYC 1 cut(s) 98
AdeI CACNNNGTG 2 cut(s) 1988, 2095
AfaI GTAC 4 cut(s) 151, 1362, 2732, 2830
AfiI CCNNNNNNNGG 2 cut(s) 1964, 1965
AflII CTTAAG 8 cut(s) 89, 188, 230, 266, 1193, 2768, 2884, 2945
AflIII ACRYGT 1 cut(s) 2563
AjnI CCWGG 2 cut(s) 2362, 2391
AleI CACNNNNGTG 1 cut(s) 2217
AloI GAACNNNNNNTCC 2 cut(s) 1700, 1732
Alw21I GWGCWC 1 cut(s) 3185
Alw26I GTCTC 9 cut(s) 89, 452, 928, 1286, 2568, 2612, 3032, 3127, 3320
AlwI GGATC 3 cut(s) 1359, 1455, 1847
AlwNI CAGNNNCTG 2 cut(s) 252, 3141
Ama87I CYCGRG 1 cut(s) 1960
Aor13HI TCCGGA 3 cut(s) 739, 1105, 2989
AoxI GGCC 4 cut(s) 114, 330, 2125, 2221
ApeKI GCWGC 6 cut(s) 288, 456, 885, 1038, 1991, 2967
ApoI RAATTY 6 cut(s) 305, 1110, 2205, 2529, 3305, 3325
AseI ATTAAT 2 cut(s) 557, 2153
Asp700I GAANNNNTTC 3 cut(s) 594, 1101, 1659
AsuHPI GGTGA 3 cut(s) 161, 1404, 2645
AvaI CYCGRG 1 cut(s) 1960
BauI CACGAG 1 cut(s) 3112
BbsI GAAGAC 2 cut(s) 659, 869
Bbv12I GWGCWC 1 cut(s) 3185
BbvI GCAGC 6 cut(s) 275, 468, 897, 1050, 1978, 2954
BceAI ACGGC 5 cut(s) 359, 586, 1205, 2808, 3089
BcgI CGANNNNNNTGC 2 cut(s) 92, 126
BciT130I CCWGG 2 cut(s) 2364, 2393
BclI TGATCA 1 cut(s) 1486
BcoDI GTCTC 9 cut(s) 89, 452, 928, 1286, 2568, 2612, 3032, 3127, 3320
BfaI CTAG 7 cut(s) 9, 672, 1004, 1739, 1757, 1854, 2453
BfmI CTRYAG 5 cut(s) 1225, 1554, 2233, 2315, 3077
BfrI CTTAAG 8 cut(s) 89, 188, 230, 266, 1193, 2768, 2884, 2945
BglII AGATCT 3 cut(s) 2017, 2261, 2455
BisI GCNGC 9 cut(s) 13, 114, 289, 457, 809, 886, 1039, 1992, 2968
BlsI GCNGC 9 cut(s) 14, 115, 290, 458, 810, 887, 1040, 1993, 2969
BmcAI AGTACT 1 cut(s) 2732
Bme1390I CCNGG 2 cut(s) 2364, 2393
BmeT110I CYCGRG 1 cut(s) 1960
BmiI GGNNCC 4 cut(s) 37, 1717, 2545, 2716
BmrFI CCNGG 2 cut(s) 2364, 2393
BmsI GCATC 6 cut(s) 24, 1408, 2062, 2482, 2857, 3147
BpiI GAAGAC 2 cut(s) 659, 869
BpmI CTGGAG 1 cut(s) 2400
BsaHI GRCGYC 1 cut(s) 98
BsaI GGTCTC 1 cut(s) 1286
BsaJI CCNNGG 4 cut(s) 341, 1344, 2392, 2790
BsaWI WCCGGW 3 cut(s) 739, 1105, 2989
BsaXI ACNNNNNCTCC 2 cut(s) 1700, 1730
Bsc4I CCNNNNNNNGG 2 cut(s) 1964, 1965
Bse3DI GCAATG 2 cut(s) 1548, 2484
BseAI TCCGGA 3 cut(s) 739, 1105, 2989
BseBI CCWGG 2 cut(s) 2364, 2393
BseDI CCNNGG 4 cut(s) 341, 1344, 2392, 2790
BseLI CCNNNNNNNGG 2 cut(s) 1964, 1965
BseMI GCAATG 2 cut(s) 1548, 2484
BseMII CTCAG 7 cut(s) 303, 783, 922, 1075, 1305, 2774, 3312
BseRI GAGGAG 1 cut(s) 1569
BseXI GCAGC 6 cut(s) 275, 468, 897, 1050, 1978, 2954
BseYI CCCAGC 1 cut(s) 1896
BsgI GTGCAG 6 cut(s) 1659, 1709, 1977, 2195, 2953, 3094
BshFI GGCC 4 cut(s) 116, 332, 2127, 2223
BsiHKAI GWGCWC 1 cut(s) 3185
BsiHKCI CYCGRG 1 cut(s) 1960
BsiSI CCGG 3 cut(s) 740, 1106, 2990
BslFI GGGAC 5 cut(s) 460, 1042, 1878, 2300, 2305
BslI CCNNNNNNNGG 2 cut(s) 1964, 1965
BsmAI GTCTC 9 cut(s) 89, 452, 928, 1286, 2568, 2612, 3032, 3127, 3320
BsmBI CGTCTC 1 cut(s) 89
BsmFI GGGAC 5 cut(s) 460, 1042, 1878, 2300, 2305
BsmI GAATGC 1 cut(s) 678
BsnI GGCC 4 cut(s) 116, 332, 2127, 2223
Bso31I GGTCTC 1 cut(s) 1286
BsoBI CYCGRG 1 cut(s) 1960
Bsp1286I GDGCHC 1 cut(s) 3185
Bsp13I TCCGGA 3 cut(s) 739, 1105, 2989
Bsp1407I TGTACA 2 cut(s) 149, 2828
Bsp143I GATC 9 cut(s) 184, 1351, 1447, 1486, 1839, 2017, 2261, 2419, 2455
BspACI CCGC 4 cut(s) 13, 113, 809, 2371
BspANI GGCC 4 cut(s) 116, 332, 2127, 2223
BspCNI CTCAG 7 cut(s) 304, 782, 921, 1074, 1304, 2773, 3313
BspEI TCCGGA 3 cut(s) 739, 1105, 2989
BspHI TCATGA 1 cut(s) 2593
BspLI GGNNCC 4 cut(s) 37, 1717, 2545, 2716
BspMAI CTGCAG 1 cut(s) 1229
BspPI GGATC 3 cut(s) 1359, 1455, 1847
BspTI CTTAAG 8 cut(s) 89, 188, 230, 266, 1193, 2768, 2884, 2945
BspTNI GGTCTC 1 cut(s) 1286
BsrDI GCAATG 2 cut(s) 1548, 2484
BsrGI TGTACA 2 cut(s) 149, 2828
BssECI CCNNGG 4 cut(s) 341, 1344, 2392, 2790
BssMI GATC 9 cut(s) 184, 1351, 1447, 1486, 1839, 2017, 2261, 2419, 2455
BssNAI GTATAC 2 cut(s) 2057, 2479
BssNI GRCGYC 1 cut(s) 98
BssSI CACGAG 1 cut(s) 3112
BssT1I CCWWGG 1 cut(s) 1344
Bst1107I GTATAC 2 cut(s) 2057, 2479
Bst2BI CACGAG 1 cut(s) 3112
Bst2UI CCWGG 2 cut(s) 2364, 2393
Bst4CI ACNGT 4 cut(s) 922, 2095, 2543, 2555
Bst6I CTCTTC 4 cut(s) 585, 3132, 3339, 3357
BstACI GRCGYC 1 cut(s) 98
BstAFI CTTAAG 8 cut(s) 89, 188, 230, 266, 1193, 2768, 2884, 2945
BstAPI GCANNNNNTGC 1 cut(s) 2000
BstAUI TGTACA 2 cut(s) 149, 2828
BstC8I GCNNGC 2 cut(s) 833, 1989
BstDSI CCRYGG 2 cut(s) 341, 2790
BstEII GGTNACC 1 cut(s) 2387
BstKTI GATC 9 cut(s) 187, 1354, 1450, 1489, 1842, 2020, 2264, 2422, 2458
BstMAI GTCTC 9 cut(s) 89, 452, 928, 1286, 2568, 2612, 3032, 3127, 3320
BstMBI GATC 9 cut(s) 184, 1351, 1447, 1486, 1839, 2017, 2261, 2419, 2455
BstMWI GCNNNNNNNGC 8 cut(s) 343, 772, 1440, 1449, 1810, 2000, 3022, 3082
BstNI CCWGG 2 cut(s) 2364, 2393
BstNSI RCATGY 3 cut(s) 2003, 2567, 2683
BstPI GGTNACC 1 cut(s) 2387
BstSCI CCNGG 2 cut(s) 2362, 2391
BstSFI CTRYAG 5 cut(s) 1225, 1554, 2233, 2315, 3077
BstV1I GCAGC 6 cut(s) 275, 468, 897, 1050, 1978, 2954
BstV2I GAAGAC 2 cut(s) 659, 869
BstX2I RGATCY 4 cut(s) 1351, 2017, 2261, 2455
BstXI CCANNNNNNTGG 2 cut(s) 1327, 2859
BstYI RGATCY 4 cut(s) 1351, 2017, 2261, 2455
BstZ17I GTATAC 2 cut(s) 2057, 2479
BsuRI GGCC 4 cut(s) 116, 332, 2127, 2223
BtgI CCRYGG 2 cut(s) 341, 2790
BtsIMutI CAGTG 1 cut(s) 3244
Cac8I GCNNGC 2 cut(s) 833, 1989
CaiI CAGNNNCTG 2 cut(s) 252, 3141
CciI TCATGA 1 cut(s) 2593
CseI GACGC 1 cut(s) 106
Csp6I GTAC 4 cut(s) 150, 1361, 2731, 2829
CspCI CAANNNNNGTGG 4 cut(s) 106, 141, 513, 548
CviQI GTAC 4 cut(s) 150, 1361, 2731, 2829
DpnI GATC 9 cut(s) 186, 1353, 1449, 1488, 1841, 2019, 2263, 2421, 2457
DpnII GATC 9 cut(s) 184, 1351, 1447, 1486, 1839, 2017, 2261, 2419, 2455
DraI TTTAAA 1 cut(s) 1882
DraIII CACNNNGTG 2 cut(s) 1988, 2095
EaeI YGGCCR 2 cut(s) 114, 330
Eam1104I CTCTTC 4 cut(s) 585, 3132, 3339, 3357
EarI CTCTTC 4 cut(s) 585, 3132, 3339, 3357
Eco130I CCWWGG 1 cut(s) 1344
Eco31I GGTCTC 1 cut(s) 1286
Eco57I CTGAAG 5 cut(s) 713, 1912, 1927, 1932, 2747
Eco88I CYCGRG 1 cut(s) 1960
Eco91I GGTNACC 1 cut(s) 2387
EcoO65I GGTNACC 1 cut(s) 2387
EcoRI GAATTC 2 cut(s) 3305, 3325
EcoRII CCWGG 2 cut(s) 2362, 2391
EcoT14I CCWWGG 1 cut(s) 1344
ErhI CCWWGG 1 cut(s) 1344
Esp3I CGTCTC 1 cut(s) 89
FalI AAGNNNNNCTT 2 cut(s) 123, 155
FaqI GGGAC 5 cut(s) 460, 1042, 1878, 2300, 2305
FauNDI CATATG 3 cut(s) 570, 684, 2934
FbaI TGATCA 1 cut(s) 1486
FblI GTMKAC 3 cut(s) 2056, 2478, 2670
Fnu4HI GCNGC 9 cut(s) 13, 114, 289, 457, 809, 886, 1039, 1992, 2968
Fsp4HI GCNGC 9 cut(s) 13, 114, 289, 457, 809, 886, 1039, 1992, 2968
FspBI CTAG 7 cut(s) 9, 672, 1004, 1739, 1757, 1854, 2453
GluI GCNGC 9 cut(s) 13, 114, 289, 457, 809, 886, 1039, 1992, 2968
GsaI CCCAGC 1 cut(s) 1900
GsuI CTGGAG 1 cut(s) 2400
HaeIII GGCC 4 cut(s) 116, 332, 2127, 2223
HapII CCGG 3 cut(s) 740, 1106, 2990
HgaI GACGC 1 cut(s) 106
Hin1I GRCGYC 1 cut(s) 98
HincII GTYRAC 4 cut(s) 467, 1049, 2412, 2671
HindII GTYRAC 4 cut(s) 467, 1049, 2412, 2671
HindIII AAGCTT 2 cut(s) 41, 2720
HpaII CCGG 3 cut(s) 740, 1106, 2990
HphI GGTGA 3 cut(s) 161, 1404, 2645
Hpy166II GTNNAC 9 cut(s) 136, 169, 467, 1049, 2057, 2412, 2479, 2558, 2671
Hpy8I GTNNAC 9 cut(s) 136, 169, 467, 1049, 2057, 2412, 2479, 2558, 2671
HpyAV CCTTC 7 cut(s) 137, 737, 968, 1613, 1951, 2171, 2705
HpyCH4III ACNGT 4 cut(s) 922, 2095, 2543, 2555
HpyCH4IV ACGT 1 cut(s) 581
HpyF10VI GCNNNNNNNGC 8 cut(s) 343, 772, 1440, 1449, 1810, 2000, 3022, 3082
HpySE526I ACGT 1 cut(s) 581
Hsp92I GRCGYC 1 cut(s) 98
Kpn2I TCCGGA 3 cut(s) 739, 1105, 2989
Ksp22I TGATCA 1 cut(s) 1486
Kzo9I GATC 9 cut(s) 184, 1351, 1447, 1486, 1839, 2017, 2261, 2419, 2455
LmnI GCTCC 5 cut(s) 1582, 1721, 2033, 2529, 3013
Lsp1109I GCAGC 6 cut(s) 275, 468, 897, 1050, 1978, 2954
LweI GCATC 6 cut(s) 24, 1408, 2062, 2482, 2857, 3147
MaeI CTAG 7 cut(s) 9, 672, 1004, 1739, 1757, 1854, 2453
MaeII ACGT 1 cut(s) 581
MalI GATC 9 cut(s) 186, 1353, 1449, 1488, 1841, 2019, 2263, 2421, 2457
MboI GATC 9 cut(s) 184, 1351, 1447, 1486, 1839, 2017, 2261, 2419, 2455
MfeI CAATTG 6 cut(s) 795, 1182, 1429, 1950, 2215, 3066
MflI RGATCY 4 cut(s) 1351, 2017, 2261, 2455
MhlI GDGCHC 1 cut(s) 3185
MlyI GAGTC 7 cut(s) 217, 454, 646, 673, 883, 1036, 3344
MmeI TCCRAC 4 cut(s) 1492, 2945, 3219, 3318
MroI TCCGGA 3 cut(s) 739, 1105, 2989
MroXI GAANNNNTTC 3 cut(s) 594, 1101, 1659
MslI CAYNNNNRTG 8 cut(s) 573, 683, 783, 1149, 2121, 2217, 2349, 2933
MspA1I CMGCKG 3 cut(s) 811, 835, 1559
MspCI CTTAAG 8 cut(s) 89, 188, 230, 266, 1193, 2768, 2884, 2945
MspI CCGG 3 cut(s) 740, 1106, 2990
MspR9I CCNGG 2 cut(s) 2364, 2393
MunI CAATTG 6 cut(s) 795, 1182, 1429, 1950, 2215, 3066
Mva1269I GAATGC 1 cut(s) 678
MvaI CCWGG 2 cut(s) 2364, 2393
MwoI GCNNNNNNNGC 8 cut(s) 343, 772, 1440, 1449, 1810, 2000, 3022, 3082
NdeI CATATG 3 cut(s) 570, 684, 2934
NdeII GATC 9 cut(s) 184, 1351, 1447, 1486, 1839, 2017, 2261, 2419, 2455
NlaIV GGNNCC 4 cut(s) 37, 1717, 2545, 2716
NmeAIII GCCGAG 1 cut(s) 358
NmuCI GTSAC 5 cut(s) 409, 1410, 1846, 1980, 2753
NspI RCATGY 3 cut(s) 2003, 2567, 2683
OliI CACNNNNGTG 1 cut(s) 2217
PaeR7I CTCGAG 1 cut(s) 1960
PagI TCATGA 1 cut(s) 2593
PciI ACATGT 1 cut(s) 2563
PctI GAATGC 1 cut(s) 678
PdmI GAANNNNTTC 3 cut(s) 594, 1101, 1659
PfeI GAWTC 8 cut(s) 235, 839, 1303, 1340, 1671, 2662, 2863, 2914
PkrI GCNGC 9 cut(s) 14, 115, 290, 458, 810, 887, 1040, 1993, 2969
PleI GAGTC 7 cut(s) 216, 453, 645, 672, 882, 1035, 3343
PpsI GAGTC 7 cut(s) 216, 453, 645, 672, 882, 1035, 3343
PscI ACATGT 1 cut(s) 2563
PshBI ATTAAT 2 cut(s) 557, 2153
Psp6I CCWGG 2 cut(s) 2362, 2391
PspEI GGTNACC 1 cut(s) 2387
PspFI CCCAGC 1 cut(s) 1896
PspGI CCWGG 2 cut(s) 2362, 2391
PspN4I GGNNCC 4 cut(s) 37, 1717, 2545, 2716
PstI CTGCAG 1 cut(s) 1229
PstNI CAGNNNCTG 2 cut(s) 252, 3141
PsuI RGATCY 4 cut(s) 1351, 2017, 2261, 2455
PvuII CAGCTG 2 cut(s) 835, 1559
RsaI GTAC 4 cut(s) 151, 1362, 2732, 2830
RsaNI GTAC 4 cut(s) 150, 1361, 2731, 2829
RseI CAYNNNNRTG 8 cut(s) 573, 683, 783, 1149, 2121, 2217, 2349, 2933
SalI GTCGAC 1 cut(s) 2669
SatI GCNGC 9 cut(s) 13, 114, 289, 457, 809, 886, 1039, 1992, 2968
Sau3AI GATC 9 cut(s) 184, 1351, 1447, 1486, 1839, 2017, 2261, 2419, 2455
ScaI AGTACT 1 cut(s) 2732
SchI GAGTC 7 cut(s) 217, 454, 646, 673, 883, 1036, 3344
ScrFI CCNGG 2 cut(s) 2364, 2393
SduI GDGCHC 1 cut(s) 3185
SfaNI GCATC 6 cut(s) 24, 1408, 2062, 2482, 2857, 3147
SfcI CTRYAG 5 cut(s) 1225, 1554, 2233, 2315, 3077
Sfr274I CTCGAG 1 cut(s) 1960
SlaI CTCGAG 1 cut(s) 1960
SmiMI CAYNNNNRTG 8 cut(s) 573, 683, 783, 1149, 2121, 2217, 2349, 2933
SsiI CCGC 4 cut(s) 13, 113, 809, 2371
SspMI CTAG 7 cut(s) 9, 672, 1004, 1739, 1757, 1854, 2453
StyD4I CCNGG 2 cut(s) 2362, 2391
StyI CCWWGG 1 cut(s) 1344
TaaI ACNGT 4 cut(s) 922, 2095, 2543, 2555
TaiI ACGT 1 cut(s) 584
TaqI TCGA 7 cut(s) 300, 588, 1731, 1961, 2462, 2670, 2841
TatI WGTACW 4 cut(s) 149, 1360, 2730, 2828
TauI GCSGC 3 cut(s) 15, 116, 811
TfiI GAWTC 8 cut(s) 235, 839, 1303, 1340, 1671, 2662, 2863, 2914
TscAI CASTG 1 cut(s) 3244
TseFI GTSAC 5 cut(s) 409, 1410, 1846, 1980, 2753
TseI GCWGC 6 cut(s) 288, 456, 885, 1038, 1991, 2967
Tsp45I GTSAC 5 cut(s) 409, 1410, 1846, 1980, 2753
TspGWI ACGGA 1 cut(s) 2096
TspRI CASTG 1 cut(s) 3244
Vha464I CTTAAG 8 cut(s) 89, 188, 230, 266, 1193, 2768, 2884, 2945
VspI ATTAAT 2 cut(s) 557, 2153
XapI RAATTY 6 cut(s) 305, 1110, 2205, 2529, 3305, 3325
XceI RCATGY 3 cut(s) 2003, 2567, 2683
XhoI CTCGAG 1 cut(s) 1960
XmiI GTMKAC 3 cut(s) 2056, 2478, 2670
XmnI GAANNNNTTC 3 cut(s) 594, 1101, 1659
XspI CTAG 7 cut(s) 9, 672, 1004, 1739, 1757, 1854, 2453
ZrmI AGTACT 1 cut(s) 2732
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.