Rw0G010340

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00435
OG0010532
Physical Location & Seq
Reverse (-)
2228 .. 8814
6587 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G010340.1

Sequence Viewer

Length: 5424 bp
ATGGCTGTTGCTGCTGATTCTTCTTCTTCTCGCATTCGTGGCAGTTATGATGTCTTCTTGAGTTTTAGAGGGTTTGACACTCGCAAGATCTTTGTTGGCCATCTTTACAATGCTCTACAACGGAAAGCACTCGCTACCTTCATTGATACTGAACAGCTTGGGAAAGGTGACGTTCTCGGGGTGCTACTGAAAGCTATTGACGCGTCAAAGCTTTCCATTGTAGTTTTATCTGAGAATTTTGCCTATTCCAAATGGTGCTTACTGGAACTGGAAAAAATCGTGGAATGCAAGGAAACAAAGAATCATATAGTGGTTCCCATTTTCTACCAAGTAAAGCCGGCTGATGTTCGTTATGTGAGTGGAAGTTTTCAGGAAGCGTTTGAAAAACACGAGCACGACTGCAGGGGGACCATTGAAGAACGGAAGCGGTGGAAGTCTGCTCTATCTAGTGCCGGCTCTATTCATGGCTGGGAATCCAGCAAATTCGAGAATGATGTCGATCTTATTGATGCTGTTGTTAAAGATATTTTAAAACAATTGAGCAACATCAGGGAAAGTAAATCCAATAATGACTTGGTTGGAATGGATTCCCATATAAGCAAAATGAAGTCTCTATTAAGTCCTTATGGGTCGGGGAATGTCTGCGTTGTCGTTGGAATATGGGGTATGGGTGGTCTAGGGAAGACAACCATCGCTAGAGCTGTTTATGAGGAAATGTGTGGAGACTTTGAGCACCATTGCTTTCTTTATAATGTCAAGGAAGATTTCAAGAGAAAAGGCGAAGCAGGAATGCAGGAAGAACTGCTATATAGAATCTTGAGGGAAAATGGCCAGCACATAAGTTCCAAAATGATAATGGAAAGGCTTAGTCGGATAAAACTTTTTCTTGTTCTTGATGATGTGGACAGTTCTGATCAAATTGATGCCCTACTTGGAAAACAAAGTTCATTTGCTCATGGAAGTCGAGTCATTATAACGACCAGAGATAAGGGCCTGCTTAGAGACTTTGAAGTATACGAACCGGCATATTTAGATAACAAACAAGCTCTTGAACTCTTTAAGAAGTATGCCTTCAGAACAATCGAATGCTCACCAAAGTACGATGATCTTTCACAGCGTGCCATAGAATATGCTCAGGGTCTGCCTTTAGCACTTAAAGTCTTGGGAGCCTCTCTCTATAAGAAAAGAGTGCGTGAGTGGGAAGTTGAATTTGTAAAAATAAAGAAATGCCAGCACTTGGGAATTGAGGGGGTACTAAGGACAACCTTTGAAGGACTAGATCATCTACAGAAAACAGTGCTTCTAGATATTGCATGTTTCTTTAGAGGAATGAAAAAGGACTATGCAACAAAAATTATGGAGGCTTGTGGCTTCTTTCCCGCAAGTGTATTACCAGTTCTAGCTGATAGAGCTCTTGTGACTAGCACTGGATACTATGTTGTGCTGACAATGCATGATTTACTATTGGACATGGCTTTGGGAATTGTCCGTCGAGAATCAAGCCTCAGTAGGTTGTCGAGTTCTGATGAAATCGAGGAGGTGTTAACTCAAAATACGGATACAAAAGCAGTTGAAAGTATAGACCTCGACTTGTACCACTCAGCAGGCATAAGCATAAATGCTAATGCTTTTGTTCACATGACGAAACTAAGACTTCTCAGAATCCGATATAATCGTTCCATAGACTTTGTTGATGATTTTTATGGTTCCCGGGGGGAATTATACCATCTAATTAATGACTGTAAACAAGAAGTGAGTGGGGAGTTAAAGTTTCTTTCTCATGAGTTGAGGTGTCTCATCTGGCATGGGTGCCCCCTGAAATCTTTGCCATCCAATTTCAACCCAAAGCATCTAGTCGACCTTGACATGCGTTCTAGCCGCATCCAACAACTATGGAAAGGAACCAAGCTTCTGAAAAAGTTGAAATACATTGATTTAAGTCTATCTCTATGCCTTAAGGAGACGCCCGACTTAACCAAGGCCACAAATCTTGAGAGAGTGAACCTTGAAGGTTGTACTAGTTTATTGGGGGTTCACCCATCCATCTTTGATCTTAAGAACCTCCTTTTCTTGAGTCTAAAAGGGTGCAAAGAGCTTAAGATTCTCCCAAGCAGCTTCCGTATGATATCTCTTGAGATCCTTGAGCTTTCTGGCTGCTCAAACCTCGAGAAATTTCCAGAGATTTCAGAAGTTATGGCGAAGCTACCACGGCTTTGTTTAGATAAGACTGCAATTAAAGAACTGCCCTCATCAATAAGAAATCTTCGAGGGCTTGTCACTTTGAGCCTAAGAGATTGCAGAGAATTTGAGACCCTCCCAAGCAGCATTTGTCAACTCAAGTCCCTTAGATATGTTACTCTCTCTGGGTGTTCTAAGTTTGAGGTGTTTCCGAAAATTATGGGGCTAGATGATATGGAAAAATTGAAAGAGCTTCATTTGGATGGAACATCAATCAAAGAGTTTTTGCCGTCAACTTCAGCTCTTAAAATCCTTGAAGTCTTGAGTCTAAGAGATTGCAAAGAGCTTAAGACTCTTCCAAGTAGCATTCATATGAGTTATCTTGACACGCTTATTCTTTCTGGATGCTCAAACCTTCAGAAGTTTCCAGAGATTTCAGACAAAATGTCTAGGCTCACACAGCTTCATTTAGATGATATTTCAATTGAAGAACTGCCGCTGTCCATTAAAAATCTTTGCCAGATGGAATCACTGAGCCTTAAAGATTGTGGAGAACTTAAGAGTCTTCCGAGCAGCATTTTTCAACTTGAGTACCTCAAAATGATTACTGTCTCTGGATGTTCGAAGTTTGAGCTGTTTCCGAAAATTGAAGATTTGGAAGGACTAGGAAAACTTGTTACATTGAGGCTAGAAGATTGTGGAGAACTTAGGACTCTGCCGAGCAGCATTTGTCAACTCAAGTCCCTTAGATTTGTTTCTCTCTCTGGTTGTTCGAAGTTTGAGGTGTTTCCGGAAATTATAGAAGATATGGAAGAATTAAGAGAGCTTCATCTTGACGGGACGTCTATTGAAGCACTTGTCCCATCAATTGATCTGCTGAAGAGGCTTATGTTATTGAACATGAGAAACTGCAAGAGCCTTCAATTACTTCCAGACAGTGTCTGTAATTTGTCATACCTTACAGGTCTCACTCTCTCAGGGTGCTCGAATCTCCATAAGTTGCCCAAGAACTTGGGGAAGTTAGAATCCTTGATGGATCTTGAAGTACAGGGAAGTGGTATAGAGCAACCCTTCTCTCCCCCATCATCATCAACCTATTGCTTGGTTGGAATGGATCGCCACATAAATGAAATGCAATTGCTATTATGTCCTGCTGAGGTGGATGATGTTCTCATTGTTGGGATATGTGGAATTGGTGGCAGTGGCAAGACAACCATTGCTACAGCTGTTTATGATAAAATTGCTCCTCAATTTGAATACCGTTGCTTTCTTGAGAATGTCAGCGAAGGTTTTACAAAGCATGGCGCAGTATATATGCAAGAAAAGCTTCTGTCTAGAATCTTACAGGAAAAGGTACAGAGTTTGGGCATTTTGAATAGAGGTTCCAATGTGATAATGGAAAGGCTAGCTAAGACAAAAGTTCTGCTCGTTCTTGATGATGTAGATGATATAAACCAGATTGAAACCTTAGTTGGAAAGCCACATTCATTTGGTGGTGGTAGTAGAATCGTTGTGACAACTAGAGAGGAGCAAATAATGAGTGGATTTCAGATGTATTCTCCCAAGCTATTAGATGATGAAGCTCTTGAACTCTTCAGGCATTTTGCCTTCAGAACAATTAAACCCTCGAAAAAGTTCAAACATCTCTCACAGCGTGCTATTGAATATGTTCAAGGTCTACCTTTAGCTCTTAAAGTCTTGGGAGGTTCTCTTTATCAGAAAAATGCACGCAAGTGGAAAGATGCCTTGAAGGAGGGGCACACAGTACATTGGGGTTTTACAACAAGCTTTTATGACCTACATCCCTCGGCGAGGGACATACTTCTAGATGTTGCATGGTTCTTTAAAGGAATGAAAAAAGAGTATGCAACCAAAATTCTGCACAGTTGTGGCTTCTTTCCTTATAGTGCATTAAGAGTTCTAGTTGATAGATCTCTCATAGCCATCTCAGATACGGACGCGACTCTCAAGATACCTGATGAACTACAGGAAATTGTTCGGGATTTCGTTCGCCATGAAGATGAACATAATCCTGGGAACCGCAGTAGATTAGAGGGTTACCAAGGAATTAGGTGTTTAACTCAAGATCAGGTTACTGGAGCAGTTGAAGTTATAACCCTTAATCTGTCAAACTCAACAGAGGCGTACTTCAATGCTGAGGCTTTTGTTACAATGAAGAAACTGAGACTACTCCAAATTTATGATAACGGTTCCATTAACTGTAAACAACATGTGATTGGAGACTTGGAGTTTATTTCTCATGAGTTGAGGTGTCTCATCTGGCATAGATACCCCCTAAAGTCTTTGCCATCCGAGTTTCACCCTCAGAATCTTGTCGACCTTGACATGTGTTTTAGTAGCCTTGAACAATTTTGGGAAGGAACTAAGCCTCTGAAAAACTTGACATTCATGAATTTAAGTCACTCTCCATGTCTCAAGAAGACCCCTGACTTGACTAAGGCTACAAAACTTGAGAAGCTAATTCTTGATGGTTGTACAAGTTTATTCGAGGTGCACCCATCCATTTGGGATCATAAAAACCTTGTCTTATTAAGTCTCAAAGGTTGCACAGAACTTAAGGTTCTTCCGGCCAGCATCCATATGAATTCTCTTAAGACCCTCAACCTTTCTGGCTGCTTAAATCTTGAGAAGCTTCCGGAGATTGCAGAAGTTATGATGGACCTATCAGAGCTTGGTTTGGATGAGACTGCAATTAAAGACCTGCCCTCATCAATCGAACGGCTTCAGGGACTTAATGTATTAAGTATGAGAAACTGCACAAGCCTTGTCTCTCTTCCGGACAGCATCTGTAGTTTGGCACACCTTACATTTCTCAATCTGTCTGGGTGCTCAAAACTTACTGACTTTCCTGACAGCTTGAGGATTATGCAATCATTGACAATCAGTGGTATAAAACAACTCCCACCCTCAATGAGAAGCAAAAGAACAGAGAGGGAGGGAGTAATTGGAGTCTCGGGGGAGGGAAGTTCATCAGAGTCTGGGGAAGGAAGAGTCTACAACCACACATTGTCGCCGGTGCCAAAGCAACATAGTGAGAGAAAAGAAGAGAAAGTCGATGGAAGTGGCAGTGTAAAGGGAGGATTGCTTGGCAGGAGGAGGGAAGTTGGGGGCCTTTTGCAGCTTCTTGAGTTTCCCGGGGGAACTATGTCTCTGAGAACGATAGAAAGAGAAAGATTAATTAGGCACAGAGGTGTACTTTTTTTTTTTTGTCTAATCTCTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

1807

Amino Acids

203.47

Weight (kDa)

6.88

Isoelectric Point (pI)

41.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 16 - 194 7.2e-46 TIR domain
TIR_2 PF13676 18 - 126 1.8e-11 TIR domain
NB-ARC PF00931 198 - 359 4.9e-17 NB-ARC domain
WHD_ROQ1 PF23282 430 - 500 1.9e-11 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 702 - 836 2.7e-13 Leucine-rich repeat region
LRR_13 PF23286 709 - 792 1.6e-13 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 845 - 956 1.3e-07 Leucine-rich repeat region
LRR_13 PF23286 853 - 936 1.1e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 954 - 1077 6.7e-11 Leucine-rich repeat region
LRR_13 PF23286 972 - 1059 2.4e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
NB-ARC PF00931 1113 - 1264 3.4e-15 NB-ARC domain
WHD_ROQ1 PF23282 1331 - 1398 2.4e-11 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 1586 - 1695 4.4e-11 Leucine-rich repeat region
LRR_13 PF23286 1594 - 1681 4.3e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 750, 974, 4292
AatII GACGTC 1 cut(s) 3023
Acc36I ACCTGC 1 cut(s) 4908
AccB1I GGYRCC 2 cut(s) 1809, 5215
AccB7I CCANNNNNTGG 1 cut(s) 1237
AccI GTMKAC 5 cut(s) 1014, 1857, 3856, 4515, 5193
AccII CGCG 2 cut(s) 203, 4139
AccIII TCCGGA 3 cut(s) 2968, 4834, 4975
AciI CCGC 5 cut(s) 427, 1380, 1879, 2675, 4219
AclWI GGATC 4 cut(s) 2131, 3222, 3300, 4716
AcoI YGGCCR 3 cut(s) 97, 829, 4767
AcsI RAATTY 9 cut(s) 235, 482, 1208, 2171, 2303, 4053, 4374, 4591, 4783
AcuI CTGAAG 7 cut(s) 1057, 2460, 2579, 3077, 3757, 3772, 4907
AcyI GRCGYC 2 cut(s) 1964, 3020
AdeI CACNNNGTG 2 cut(s) 1118, 3833
AfiI CCNNNNNNNGG 2 cut(s) 1237, 3990
AflII CTTAAG 7 cut(s) 1955, 2054, 2096, 2525, 2735, 4754, 4790
AflIII ACRYGT 3 cut(s) 201, 4408, 4524
AhdI GACNNNNNGTC 2 cut(s) 2623, 3019
AhlI ACTAGT 1 cut(s) 2018
AjnI CCWGG 1 cut(s) 4210
AjuI GAANNNNNNNTTGG 2 cut(s) 3633, 3665
AleI CACNNNNGTG 3 cut(s) 3910, 4065, 5388
Alw21I GWGCWC 6 cut(s) 396, 735, 1414, 3164, 4695, 5030
Alw44I GTGCAC 1 cut(s) 4691
AlwI GGATC 4 cut(s) 2131, 3222, 3300, 4716
AlwNI CAGNNNCTG 4 cut(s) 1141, 3120, 4986, 5177
Ama87I CYCGRG 5 cut(s) 176, 1710, 2165, 5152, 5333
Aor13HI TCCGGA 3 cut(s) 2968, 4834, 4975
AoxI GGCC 6 cut(s) 97, 829, 991, 1980, 4767, 5308
ApaLI GTGCAC 1 cut(s) 4691
ApeKI GCWGC 8 cut(s) 11, 2112, 2154, 2322, 2751, 2901, 4812, 5317
ApoI RAATTY 9 cut(s) 235, 482, 1208, 2171, 2303, 4053, 4374, 4591, 4783
AseI ATTAAT 2 cut(s) 1734, 5375
Asp700I GAANNNNTTC 6 cut(s) 586, 3504, 3812, 3846, 4173, 4920
AspLEI GCGC 1 cut(s) 3485
AspS9I GGNCC 4 cut(s) 408, 991, 4858, 5308
AsuC2I CCSGG 4 cut(s) 1711, 1712, 5334, 5335
AsuHPI GGTGA 4 cut(s) 179, 1083, 2027, 4490
AsuII TTCGAA 2 cut(s) 2801, 2951
AsuNHI GCTAGC 1 cut(s) 3583
AvaI CYCGRG 5 cut(s) 176, 1710, 2165, 5152, 5333
AvaII GGWCC 2 cut(s) 408, 4858
BaeGI GKGCMC 3 cut(s) 1814, 3939, 4695
BaeI ACNNNNGTAYC 2 cut(s) 2753, 2786
BalI TGGCCA 2 cut(s) 99, 831
BanI GGYRCC 2 cut(s) 1809, 5215
BanII GRGCYC 1 cut(s) 1414
BauI CACGAG 1 cut(s) 389
BbsI GAAGAC 4 cut(s) 46, 689, 2735, 4625
Bbv12I GWGCWC 6 cut(s) 396, 735, 1414, 3164, 4695, 5030
BbvCI CCTCAGC 2 cut(s) 3333, 4335
BbvI GCAGC 7 cut(s) 2124, 2141, 2334, 2763, 2913, 4799, 5329
BceAI ACGGC 3 cut(s) 2225, 2452, 4934
BciT130I CCWGG 1 cut(s) 4212
BciVI GTATCC 2 cut(s) 1424, 1552
BclI TGATCA 1 cut(s) 913
BcnI CCSGG 4 cut(s) 1711, 1712, 5334, 5335
BcuI ACTAGT 1 cut(s) 2018
BfmI CTRYAG 5 cut(s) 400, 1286, 3399, 4163, 4987
BfrI CTTAAG 7 cut(s) 1955, 2054, 2096, 2525, 2735, 4754, 4790
BfuAI ACCTGC 1 cut(s) 4908
BfuI GTATCC 2 cut(s) 1424, 1552
BglII AGATCT 2 cut(s) 87, 4109
Bme1390I CCNGG 5 cut(s) 1711, 1712, 4212, 5334, 5335
Bme18I GGWCC 2 cut(s) 408, 4858
BmeRI GACNNNNNGTC 2 cut(s) 2623, 3019
BmeT110I CYCGRG 5 cut(s) 176, 1710, 2165, 5152, 5333
BmgT120I GGNCC 4 cut(s) 408, 991, 4858, 5308
BmrFI CCNGG 5 cut(s) 1711, 1712, 4212, 5334, 5335
BmsI GCATC 8 cut(s) 499, 913, 1858, 1890, 2573, 3910, 4782, 4992
BmtI GCTAGC 1 cut(s) 3587
BpiI GAAGAC 4 cut(s) 46, 689, 2735, 4625
BplI GAGNNNNNCTC 2 cut(s) 1158, 1190
BpmI CTGGAG 1 cut(s) 4296
Bpu10I CCTNAGC 3 cut(s) 1134, 3333, 4335
Bpu14I TTCGAA 2 cut(s) 2801, 2951
BpuMI CCSGG 4 cut(s) 1711, 1712, 5334, 5335
BsaBI GATNNNNATC 1 cut(s) 498
BsaHI GRCGYC 2 cut(s) 1964, 3020
BsaI GGTCTC 2 cut(s) 2303, 3149
BsaJI CCNNGG 9 cut(s) 1710, 1711, 1977, 2207, 3984, 4211, 4240, 5333, 5334
BsaWI WCCGGW 3 cut(s) 2968, 4834, 4975
BsaXI ACNNNNNCTCC 2 cut(s) 1754, 1784
Bsc4I CCNNNNNNNGG 2 cut(s) 1237, 3990
Bse118I RCCGGY 4 cut(s) 337, 452, 1021, 5212
Bse1I ACTGG 5 cut(s) 267, 273, 1394, 1432, 4279
Bse3DI GCAATG 2 cut(s) 736, 3393
Bse8I GATNNNNATC 1 cut(s) 498
BseAI TCCGGA 3 cut(s) 2968, 4834, 4975
BseBI CCWGG 1 cut(s) 4212
BseDI CCNNGG 9 cut(s) 1710, 1711, 1977, 2207, 3984, 4211, 4240, 5333, 5334
BseJI GATNNNNATC 1 cut(s) 498
BseLI CCNNNNNNNGG 2 cut(s) 1237, 3990
BseMI GCAATG 2 cut(s) 736, 3393
BseNI ACTGG 5 cut(s) 267, 273, 1394, 1432, 4279
BseRI GAGGAG 4 cut(s) 1550, 3414, 3719, 5308
BseSI GKGCMC 3 cut(s) 1814, 3939, 4695
BseXI GCAGC 7 cut(s) 2124, 2141, 2334, 2763, 2913, 4799, 5329
BseYI CCCAGC 1 cut(s) 468
BsgI GTGCAG 2 cut(s) 4043, 4939
Bsh1236I CGCG 2 cut(s) 203, 4139
BshFI GGCC 6 cut(s) 99, 831, 993, 1982, 4769, 5310
BshNI GGYRCC 2 cut(s) 1809, 5215
BsiHKAI GWGCWC 6 cut(s) 396, 735, 1414, 3164, 4695, 5030
BsiHKCI CYCGRG 5 cut(s) 176, 1710, 2165, 5152, 5333
BslFI GGGAC 7 cut(s) 421, 2326, 2905, 3023, 3031, 4007, 4941
BslI CCNNNNNNNGG 2 cut(s) 1237, 3990
BsmBI CGTCTC 1 cut(s) 1955
BsmFI GGGAC 7 cut(s) 421, 2326, 2905, 3023, 3031, 4007, 4941
BsmI GAATGC 5 cut(s) 33, 290, 795, 1091, 2544
BsnI GGCC 6 cut(s) 99, 831, 993, 1982, 4769, 5310
Bso31I GGTCTC 2 cut(s) 2303, 3149
BsoBI CYCGRG 5 cut(s) 176, 1710, 2165, 5152, 5333
Bsp119I TTCGAA 2 cut(s) 2801, 2951
Bsp1286I GDGCHC 8 cut(s) 396, 735, 1414, 1814, 3164, 3939, 4695, 5030
Bsp13I TCCGGA 3 cut(s) 2968, 4834, 4975
Bsp1407I TGTACA 1 cut(s) 4673
BspACI CCGC 5 cut(s) 427, 1380, 1879, 2675, 4219
BspANI GGCC 6 cut(s) 99, 831, 993, 1982, 4769, 5310
BspEI TCCGGA 3 cut(s) 2968, 4834, 4975
BspFNI CGCG 2 cut(s) 203, 4139
BspHI TCATGA 3 cut(s) 1780, 4438, 4587
BspMAI CTGCAG 1 cut(s) 404
BspMI ACCTGC 1 cut(s) 4908
BspOI GCTAGC 1 cut(s) 3587
BspPI GGATC 4 cut(s) 2131, 3222, 3300, 4716
BspT104I TTCGAA 2 cut(s) 2801, 2951
BspT107I GGYRCC 2 cut(s) 1809, 5215
BspTI CTTAAG 7 cut(s) 1955, 2054, 2096, 2525, 2735, 4754, 4790
BspTNI GGTCTC 2 cut(s) 2303, 3149
BsrDI GCAATG 2 cut(s) 736, 3393
BsrFI RCCGGY 4 cut(s) 337, 452, 1021, 5212
BsrGI TGTACA 1 cut(s) 4673
BsrI ACTGG 5 cut(s) 267, 273, 1394, 1432, 4279
BssAI RCCGGY 4 cut(s) 337, 452, 1021, 5212
BssECI CCNNGG 9 cut(s) 1710, 1711, 1977, 2207, 3984, 4211, 4240, 5333, 5334
BssNAI GTATAC 1 cut(s) 1015
BssNI GRCGYC 2 cut(s) 1964, 3020
BssSI CACGAG 1 cut(s) 389
BssT1I CCWWGG 2 cut(s) 1977, 4240
Bst1107I GTATAC 1 cut(s) 1015
Bst2BI CACGAG 1 cut(s) 389
Bst2UI CCWGG 1 cut(s) 4212
Bst6I CTCTTC 6 cut(s) 2538, 3053, 3776, 4977, 5182, 5238
BstACI GRCGYC 2 cut(s) 1964, 3020
BstAFI CTTAAG 7 cut(s) 1955, 2054, 2096, 2525, 2735, 4754, 4790
BstAUI TGTACA 1 cut(s) 4673
BstBI TTCGAA 2 cut(s) 2801, 2951
BstDSI CCRYGG 1 cut(s) 2207
BstEII GGTNACC 1 cut(s) 4235
BstENI CCTNNNNNAGG 1 cut(s) 3988
BstFNI CGCG 2 cut(s) 203, 4139
BstHHI GCGC 1 cut(s) 3485
BstMWI GCNNNNNNNGC 9 cut(s) 11, 39, 200, 1409, 1450, 2209, 2638, 3061, 3502
BstNI CCWGG 1 cut(s) 4212
BstNSI RCATGY 4 cut(s) 1317, 1870, 4412, 4528
BstPI GGTNACC 1 cut(s) 4235
BstSCI CCNGG 5 cut(s) 1709, 1710, 4210, 5332, 5333
BstSFI CTRYAG 5 cut(s) 400, 1286, 3399, 4163, 4987
BstSLI GKGCMC 3 cut(s) 1814, 3939, 4695
BstUI CGCG 2 cut(s) 203, 4139
BstV1I GCAGC 7 cut(s) 2124, 2141, 2334, 2763, 2913, 4799, 5329
BstV2I GAAGAC 4 cut(s) 46, 689, 2735, 4625
BstX2I RGATCY 4 cut(s) 87, 2136, 3214, 4109
BstXI CCANNNNNNTGG 2 cut(s) 3190, 4704
BstYI RGATCY 4 cut(s) 87, 2136, 3214, 4109
BstZ17I GTATAC 1 cut(s) 1015
BsuI GTATCC 2 cut(s) 1424, 1552
BsuRI GGCC 6 cut(s) 99, 831, 993, 1982, 4769, 5310
BtgI CCRYGG 1 cut(s) 2207
BtgZI GCGATG 1 cut(s) 676
BtsI GCAGTG 2 cut(s) 3385, 5272
BtsIMutI CAGTG 7 cut(s) 1302, 1425, 2708, 3121, 3385, 5089, 5272
BveI ACCTGC 1 cut(s) 4908
CaiI CAGNNNCTG 4 cut(s) 1141, 3120, 4986, 5177
CciI TCATGA 3 cut(s) 1780, 4438, 4587
CfoI GCGC 1 cut(s) 3485
Cfr10I RCCGGY 4 cut(s) 337, 452, 1021, 5212
Cfr13I GGNCC 4 cut(s) 408, 991, 4858, 5308
Cfr9I CCCGGG 2 cut(s) 1710, 5333
CseI GACGC 4 cut(s) 192, 209, 1972, 4145
CspCI CAANNNNNGTGG 2 cut(s) 1972, 2007
DraI TTTAAA 2 cut(s) 531, 4024
DraIII CACNNNGTG 2 cut(s) 1118, 3833
DriI GACNNNNNGTC 2 cut(s) 2623, 3019
EaeI YGGCCR 3 cut(s) 97, 829, 4767
Eam1104I CTCTTC 6 cut(s) 2538, 3053, 3776, 4977, 5182, 5238
Eam1105I GACNNNNNGTC 2 cut(s) 2623, 3019
EarI CTCTTC 6 cut(s) 2538, 3053, 3776, 4977, 5182, 5238
Ecl136II GAGCTC 1 cut(s) 1412
Eco130I CCWWGG 2 cut(s) 1977, 4240
Eco24I GRGCYC 1 cut(s) 1414
Eco31I GGTCTC 2 cut(s) 2303, 3149
Eco32I GATATC 1 cut(s) 2127
Eco47I GGWCC 2 cut(s) 408, 4858
Eco53kI GAGCTC 1 cut(s) 1412
Eco57I CTGAAG 7 cut(s) 1057, 2460, 2579, 3077, 3757, 3772, 4907
Eco88I CYCGRG 5 cut(s) 176, 1710, 2165, 5152, 5333
Eco91I GGTNACC 1 cut(s) 4235
EcoICRI GAGCTC 1 cut(s) 1412
EcoNI CCTNNNNNAGG 1 cut(s) 3988
EcoO109I RGGNCCY 2 cut(s) 991, 5308
EcoO65I GGTNACC 1 cut(s) 4235
EcoRI GAATTC 1 cut(s) 4783
EcoRII CCWGG 1 cut(s) 4210
EcoRV GATATC 1 cut(s) 2127
EcoT14I CCWWGG 2 cut(s) 1977, 4240
EcoT22I ATGCAT 1 cut(s) 1455
EcoT38I GRGCYC 1 cut(s) 1414
ErhI CCWWGG 2 cut(s) 1977, 4240
Esp3I CGTCTC 1 cut(s) 1955
FalI AAGNNNNNCTT 4 cut(s) 1055, 1087, 3489, 3521
FaqI GGGAC 7 cut(s) 421, 2326, 2905, 3023, 3031, 4007, 4941
FauI CCCGC 1 cut(s) 1387
FauNDI CATATG 2 cut(s) 2550, 4779
FbaI TGATCA 1 cut(s) 913
FblI GTMKAC 5 cut(s) 1014, 1857, 3856, 4515, 5193
FriOI GRGCYC 1 cut(s) 1414
GlaI GCGC 1 cut(s) 3484
GsaI CCCAGC 1 cut(s) 472
GsuI CTGGAG 1 cut(s) 4296
HaeIII GGCC 6 cut(s) 99, 831, 993, 1982, 4769, 5310
HgaI GACGC 4 cut(s) 192, 209, 1972, 4145
HhaI GCGC 1 cut(s) 3485
Hin1I GRCGYC 2 cut(s) 1964, 3020
Hin6I GCGC 1 cut(s) 3483
HinP1I GCGC 1 cut(s) 3483
HincII GTYRAC 6 cut(s) 1545, 1858, 2333, 2472, 2912, 4516
HindII GTYRAC 6 cut(s) 1545, 1858, 2333, 2472, 2912, 4516
HindIII AAGCTT 5 cut(s) 209, 1907, 3503, 3964, 4829
HpaI GTTAAC 1 cut(s) 1545
HphI GGTGA 4 cut(s) 179, 1083, 2027, 4490
Hpy99I CGWCG 1 cut(s) 1494
HpyCH4IV ACGT 2 cut(s) 171, 3020
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 39, 200, 1409, 1450, 2209, 2638, 3061, 3502
HpySE526I ACGT 2 cut(s) 171, 3020
Hsp92I GRCGYC 2 cut(s) 1964, 3020
HspAI GCGC 1 cut(s) 3483
Kpn2I TCCGGA 3 cut(s) 2968, 4834, 4975
KroI GCCGGC 2 cut(s) 337, 452
KroNI GCCGGC 2 cut(s) 339, 454
Ksp22I TGATCA 1 cut(s) 913
KspAI GTTAAC 1 cut(s) 1545
LmnI GCTCC 4 cut(s) 1166, 3427, 3706, 4277
Lsp1109I GCAGC 7 cut(s) 2124, 2141, 2334, 2763, 2913, 4799, 5329
LweI GCATC 8 cut(s) 499, 913, 1858, 1890, 2573, 3910, 4782, 4992
MaeII ACGT 2 cut(s) 171, 3020
MfeI CAATTG 4 cut(s) 536, 2661, 3045, 3314
MflI RGATCY 4 cut(s) 87, 2136, 3214, 4109
MhlI GDGCHC 8 cut(s) 396, 735, 1414, 1814, 3164, 3939, 4695, 5030
MlsI TGGCCA 2 cut(s) 99, 831
MluI ACGCGT 1 cut(s) 201
MluNI TGGCCA 2 cut(s) 99, 831
MmeI TCCRAC 6 cut(s) 559, 634, 851, 1909, 3265, 3631
Mox20I TGGCCA 2 cut(s) 99, 831
Mph1103I ATGCAT 1 cut(s) 1455
MroI TCCGGA 3 cut(s) 2968, 4834, 4975
MroNI GCCGGC 2 cut(s) 337, 452
MroXI GAANNNNTTC 6 cut(s) 586, 3504, 3812, 3846, 4173, 4920
MscI TGGCCA 2 cut(s) 99, 831
MslI CAYNNNNRTG 9 cut(s) 2439, 2549, 2649, 3303, 3910, 4065, 4197, 4778, 5388
Msp20I TGGCCA 2 cut(s) 99, 831
MspA1I CMGCKG 2 cut(s) 2677, 3404
MspCI CTTAAG 7 cut(s) 1955, 2054, 2096, 2525, 2735, 4754, 4790
MspR9I CCNGG 5 cut(s) 1711, 1712, 4212, 5334, 5335
MunI CAATTG 4 cut(s) 536, 2661, 3045, 3314
Mva1269I GAATGC 5 cut(s) 33, 290, 795, 1091, 2544
MvaI CCWGG 1 cut(s) 4212
MvnI CGCG 2 cut(s) 203, 4139
MwoI GCNNNNNNNGC 9 cut(s) 11, 39, 200, 1409, 1450, 2209, 2638, 3061, 3502
NaeI GCCGGC 2 cut(s) 339, 454
NciI CCSGG 4 cut(s) 1711, 1712, 5334, 5335
NdeI CATATG 2 cut(s) 2550, 4779
NgoMIV GCCGGC 2 cut(s) 337, 452
NheI GCTAGC 1 cut(s) 3583
NmeAIII GCCGAG 2 cut(s) 2922, 3965
NmuCI GTSAC 5 cut(s) 167, 1417, 2275, 3691, 4598
NsiI ATGCAT 1 cut(s) 1455
NspI RCATGY 4 cut(s) 1317, 1870, 4412, 4528
NspV TTCGAA 2 cut(s) 2801, 2951
OliI CACNNNNGTG 3 cut(s) 3910, 4065, 5388
PaeR7I CTCGAG 1 cut(s) 2165
PagI TCATGA 3 cut(s) 1780, 4438, 4587
PciI ACATGT 2 cut(s) 4408, 4524
PctI GAATGC 5 cut(s) 33, 290, 795, 1091, 2544
PdiI GCCGGC 2 cut(s) 339, 454
PdmI GAANNNNTTC 6 cut(s) 586, 3504, 3812, 3846, 4173, 4920
PflFI GACNNNGTC 1 cut(s) 3116
PflMI CCANNNNNTGG 1 cut(s) 1237
PscI ACATGT 2 cut(s) 4408, 4524
PshBI ATTAAT 2 cut(s) 1734, 5375
PsiI TTATAA 3 cut(s) 750, 974, 4292
Psp124BI GAGCTC 1 cut(s) 1414
Psp6I CCWGG 1 cut(s) 4210
PspEI GGTNACC 1 cut(s) 4235
PspFI CCCAGC 1 cut(s) 468
PspGI CCWGG 1 cut(s) 4210
PspPI GGNCC 4 cut(s) 408, 991, 4858, 5308
PsrI GAACNNNNNNTAC 2 cut(s) 1380, 1412
PstI CTGCAG 1 cut(s) 404
PstNI CAGNNNCTG 4 cut(s) 1141, 3120, 4986, 5177
PsuI RGATCY 4 cut(s) 87, 2136, 3214, 4109
PsyI GACNNNGTC 1 cut(s) 3116
PvuII CAGCTG 1 cut(s) 3404
RseI CAYNNNNRTG 9 cut(s) 2439, 2549, 2649, 3303, 3910, 4065, 4197, 4778, 5388
SacI GAGCTC 1 cut(s) 1414
SalI GTCGAC 2 cut(s) 1856, 4514
Sau96I GGNCC 4 cut(s) 408, 991, 4858, 5308
ScrFI CCNGG 5 cut(s) 1711, 1712, 4212, 5334, 5335
SduI GDGCHC 8 cut(s) 396, 735, 1414, 1814, 3164, 3939, 4695, 5030
SfaNI GCATC 8 cut(s) 499, 913, 1858, 1890, 2573, 3910, 4782, 4992
SfcI CTRYAG 5 cut(s) 400, 1286, 3399, 4163, 4987
Sfr274I CTCGAG 1 cut(s) 2165
SfuI TTCGAA 2 cut(s) 2801, 2951
SgrAI CRCCGGYG 1 cut(s) 5212
SinI GGWCC 2 cut(s) 408, 4858
SlaI CTCGAG 1 cut(s) 2165
SmaI CCCGGG 2 cut(s) 1712, 5335
SmiMI CAYNNNNRTG 9 cut(s) 2439, 2549, 2649, 3303, 3910, 4065, 4197, 4778, 5388
SpeI ACTAGT 1 cut(s) 2018
SsiI CCGC 5 cut(s) 427, 1380, 1879, 2675, 4219
SstI GAGCTC 1 cut(s) 1414
StyD4I CCNGG 5 cut(s) 1709, 1710, 4210, 5332, 5333
StyI CCWWGG 2 cut(s) 1977, 4240
TaiI ACGT 2 cut(s) 174, 3023
TatI WGTACW 5 cut(s) 2015, 3223, 3943, 4673, 5392
TauI GCSGC 2 cut(s) 1881, 2677
TscAI CASTG 7 cut(s) 1302, 1432, 2715, 3121, 3385, 5089, 5272
TseFI GTSAC 5 cut(s) 167, 1417, 2275, 3691, 4598
TseI GCWGC 8 cut(s) 11, 2112, 2154, 2322, 2751, 2901, 4812, 5317
Tsp45I GTSAC 5 cut(s) 167, 1417, 2275, 3691, 4598
TspGWI ACGGA 6 cut(s) 136, 436, 1478, 1571, 2108, 4148
TspMI CCCGGG 2 cut(s) 1710, 5333
TspRI CASTG 7 cut(s) 1302, 1432, 2715, 3121, 3385, 5089, 5272
Tth111I GACNNNGTC 1 cut(s) 3116
Van91I CCANNNNNTGG 1 cut(s) 1237
Vha464I CTTAAG 7 cut(s) 1955, 2054, 2096, 2525, 2735, 4754, 4790
VneI GTGCAC 1 cut(s) 4691
VpaK11BI GGWCC 2 cut(s) 408, 4858
VspI ATTAAT 2 cut(s) 1734, 5375
XagI CCTNNNNNAGG 1 cut(s) 3988
XapI RAATTY 9 cut(s) 235, 482, 1208, 2171, 2303, 4053, 4374, 4591, 4783
XbaI TCTAGA 3 cut(s) 1303, 3512, 4003
XceI RCATGY 4 cut(s) 1317, 1870, 4412, 4528
XcmI CCANNNNNNNNNTGG 3 cut(s) 571, 853, 3571
XhoI CTCGAG 1 cut(s) 2165
XmaI CCCGGG 2 cut(s) 1710, 5333
XmiI GTMKAC 5 cut(s) 1014, 1857, 3856, 4515, 5193
XmnI GAANNNNTTC 6 cut(s) 586, 3504, 3812, 3846, 4173, 4920
ZraI GACGTC 1 cut(s) 3021
Zsp2I ATGCAT 1 cut(s) 1455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.