RchiOBHm_Chr1g0375971

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0010532
Physical Location & Seq
Forward (+)
63246739 .. 63255090
8352 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ59965

Sequence Viewer

Length: 5406 bp
ATGGCAGCTGCTGCTGCTGATGATTCTTCTTCTTCTTCTTCTCGCATTCGTGGCAGTTATGATGTCTTCTTGAGTTTTAGAGGGTTTGACACTCGCAAGATCTTTGTTGGCCATCTTTACAATGCTCTACAAAGGAAAGCACTCGCTACCTTCATAGATACTGAACAGCTTGGAAAAGGGGACGTTCTCGGGGTGCTACTGAAAGCTATTGACGCGTCAAAGCTTTCGGTGGTAGTTTTATCTGAGAACTTTGCCTATTCCAAATGGTGCTTACTGGAACTCGAAAAAATCGTGGAATGCATGGAAACAAAGAATCATATAGTGGTTCCCATTTTCTACCAAGTAGATCCGGCTGATGTTCGTTATGTGAGGGGAAGTTTCAGCAAAGCGTTTGAACAACATGAGCACAAGTCCAGGGGGACCCCCGAAGAACGGAAGCGGTGGAAGTCTGCTCTAATTAGAGCCGGCTATATTAATGGCTGGGATTCCCGCAAATACGAGGATGATGTCAAGCTTATTGATGATGTTGTTAAAAATGTTTTAAAACAGTTGAGCAACATCAGGGAAAGTAAATCCAATGATGACTTGGTTGGAATGGATTCCCACATAAGCAACATGAAGTCTCTGTTAAGTGCTTATGGGTCGGGGGATATCTGCATTGTTGGAATATGGGGTATGGGTGGTATAGGGAAGACAACCATCGCTAGAGCTGTTTATGAGGAAATGTGTGGAAACTTTGATCACCATTGCTTTCTTTATAATGTCAAGGAAGATTTCAAGAGAAAAGGTGAAGCAGGAATGCAGGAAGAACTTCTATATAGAATCTCAAGGGAAAATGGGCAGCACTTAAGTTCCAAAATGATTATGGAAAGGCTTAGTGGGATAAAACTTTTTGTTGTTCTCGATGATGTGGACAGTTCTGATCAAATTGATGCCCTACTTGGAAAACAATGTTCATTTGGTCGTGGAAGTAGAATCATCATAACGACCAGAGATAAGGGCCTACTTAGAGAATTTGATGTATACGAACCGGGATATTTAGATGAAGATCAAGCTCTTGAACTCTTTAAGAAGTATGCCTTCAGAACAATCGAACCCTCAACAGAGTACGATCATCTCTCACAGAGTGCCATAAAATATGCTCAAGGTCTGCCTTTAGCACTTAAAGTCTTGGGAGCCTCTCTCTATAAGAAAAGTGTACGTGAGTGGGAAGTTGAGTTTGAAAAAATAAAGAAATGCCAGCACCTGGGAATTGAGGGTGTACTAAGGACAACCTACGAAGGACTAGATCATCTACAGAAAACAGTACTTCTAGATATTGCATGTTTCTTTAAAGAAATGAAAAAGGACTATGCAACAGAAATTCTGGAGGCTTGTGGCTTCTTTCCCATAAGTGTATTACCAGTTCTCGTTGATAGAGCTCTTGTGACAAGCACTGGATACTATGTTGTGTTGACAATGCATGATTTACTATTGGACATGGCCTTGGGAATTGTCCGTCGAGAATCAAGCCTCAGTAGGTTGACGAGTTCTGATGAAATTGAGGAGGTGTTAACTCAAAACATGGATACGAAAGCAGTTGAAAGTATAGACCTCGACTTGTACCACTCAAAAGGCATACGCATAAATGCTAAAGCTTTTGTTCGCATGACGAAACTAAGACTGCTTAGAATCCGATATAACCGATCCATAGACTTTGTTGATGATTATTATGGTTCACGGGGGGAATTATACCATCTAATTGATGACTGTAAACAACAAGTGAGTGGAGAGTTAAAGTTCCTTTCTCACGAGTTGAGGTGTCTCATCTGGCATGGATGCCCCCTGAAATCTTTGCCATCCAATTTCAACCCAAAGCATTTAGTTGACCTTGACATGCGTTCTAGCCGCATCAGACAACTTTGGAAAGGAACCAAGCTTCTGAAGAAGTTGAAATACATTGATTTGAGTCTATCTCTATACCTCAAGGAGACGCCCGACTTAACTGAGGCCACAAATCTTGAGAAAGTAAACCTTGAAGGTTGTACAAGTTTATTGGAGGTTCACCCATCCATCTCTGATCTTAAGAACCTCGCTTTCTTGAGTCTAAAAGGGTGCAAAGAGCTTAAGATTCTTCCAAGCAGCTTCTGTATGGAATCTCTTAAGACCCTTGAGCTTTCTGGCTGCTCAAATGTCGACAAATTTCCTGAGATTTCAGAAGTTATGGCCGAGCTACCACGGCTTTCTTTAGATAAGACTGCAATTAAAGAACTGCCCTCATCAATAAAAAATCTTAGGGGGCTTGTCACTTTGAGCCTAAAAGATTGCAGAGAACTTGAGAGTCTCCCAAGCAGCATTTGTCAACTCAAGTCCCTTAGATATGTTACTCTCTCTGGGTGTTCTAAGTTTAAGGTGTTTCCAAAAATTGTGGGGGTAGAAGATATGAAAGGATTAATAGAGCTTCACATGGATGGAACGTCTATCGAAGAGTTTCTGCCATCAATTTCAGCTCTTAAAGAACTTGTTGTCTTGAGTCTAAGAGATTGCAAACAGCTAAAGAGTCTTCCTAGTAGCATTCATATGAAATCTCTTGACACACTTATTCTTTCTGGATGTTCGAACCTTCAGAAGTTTCCGGAGATTTCAGAAAAAATGTGGAGGCTCTCTGAGCTTCATTTAGATGATGTTGCAATTGAAGAACTGCCGCTGTCAATTAAAAACCTTTGCCAGCTGGAATCTTTGAGCCTTAAAGATTGTGGAGAACTCAAGAGTCTTCCAAGCAGCATTTTTGAACTTGAGAGCCTCAGTATGATTACTGTCTCTGGTTGTTCAAAGTTTAAGGTGTTTCCACAAAATGAAGAAGATTTGGAAGGACTACAAAAACTTGTTACATTGAGGCTAGAAGATTGTGGAGAACTTAGGAGTCTGCCGAGCAGCATTTGTCAACTCAAGTCCCTCAGATTTGTTGCTCTCTCTGGTTGTTCAAAGTTTGAAGTGTTTCCGGAAATTTTAGAAGCTATGGAAGAATTAAGAGAGCTTCATTTGGATGGAACATCTGTCAAAGACCTTTCCCCATCAATTGAACGGCTTAAGAGGATTATGTTATTGAACATGAGAAACTGCAGAAGCCTCATCTTTCTTCCTGAAAATATCTGTAATTTGTCATACCTTACAGGTCTCACTCTCTCAGGGTGCTTGAATCTCCATAACTTGCCCAAGAACTTGGGGAAATTAGAATCCTTGGTGGATCTTGAAGTACAGGGAAGCGGTATAGAGCAACCATTCTCTCTCTTGCAACCAAAGAAGTCACCATCATCATCAGCCAATTGCTTAGTTGGCATGGATCGCCATATAAATAAAATGCAATCGCTATTATGTCTTGATCAGGTGGATGATGTTCGTATTGTTGGAATATGTGGAATTGGGGGTATCGGCAAGACAACCATTGCTACAGCTGTATATGATAAAATTGCTCCTCAATTTGAACACCACTGTTTTCTTGAGAATGTCAAGGAAGGTTTCACAAAGCATAGTGCAGAACAAATGCAGGAAGAACTTCTGACCAGAATCTTAAAGGTAAAGGTGCAGAGTTTAAGCATTTTGAACAGAGGCTCCAATATGCTTATCGAAAGGCTAGGTACGAAAAAAGTTCTAGTTGTTCTTGATGATATAGACGATATAACCCAGATTGAAACCTTGCTTGAAAAGCCATATTCATTTGGTGGTGGGAGTAGGATCATTGTAACAACTAGAAAGGAAGAACTAATGAGTGGATTTAAAATATATCGTCCCCAGCTATCAAATGCTGAAGCTGTTGAAATCTTCAGACATTTTGCCTTCAGAACAATTGAACCCTCGAGAGGGTATGATGATCTGTCACAGCGTGCCGCTCAACATGCTCAAGTGCCTTTAGATCTTAAAGTCTGGGGAGCTTTTCTTTTCAACAAAAGTATACATGAGTGGGAAGATGCCTTGACGGATAAGCACACGGTGTATTGGGTGCTAACAACACACTTTTATGGCCTACATTACTCGGAGAGGAACATATTAATAGATGTTGCATGGTTCTTTGAAGGAATGAAAAAAGAGTATGCAACAAAAATTCTGCACAATTGTGGCCTCTTTCCCTATAGTGCATTAAGAGGTCTAATTGATAGATCTCTCGTATCTATATCAGAAAGGGACGGGACTATCAAACTGCATGATTTACTACAGGGGATTGTTCGGGATTGTGTTCGCCACGAAGATGAACATGAACCTGGAAACCGCAGTAGATTAGAGGGTTACCAGGGAATTAGGTGTTTGTCAACTCCAGATCAGGTTACTAAAGCAGTTGAAGTTGTAACCCTTGATCTGTCGAACTCAAAAGAGGTATACTTCATTGCAGAGGCTTTTGTTACAATGAAGAAATTAAGACTGCTCCGACTTTATGATAACGGCTCCATTAACTGTAAACAACATGTGATTGGAGACTTTGAGTTTATTTCTCATGAGTTGAGGTGTCTCATCTGGCATAGATACCCCGTAAAGTCTTTGCCATCCAATTTTCACCCAAAGAATCTTGTCGACCTTGACATGCGTTTTAGTCGCCTTGAACTACTTTGGGAAGGAACCAAGCTTCTGAAGTACTTGACATTCATCAATTTAAGTCACTCTCAGTATCTTAAGGAAATCCCCGACTTAACAACGGCTACAAATCTTGAGAAGCTAATTCTTGATGGTTGTACAAGTTTATTCGAGGTGCATCCATCCATTTGGAATCGTAAAAACCTCATTTTCTTAAGTCTAAAAGGTTGCAGACAACTTGAGATTCTTCCAACCAACATCCATATGAAATCTCTTAAGACCCTTAATCTTTCTGGCTGCACAAATCTTGAGAAGTTTCCGGAGATTTCAGAAGTTATGATGGAGCTATCAGAGCTTGGTTTAGATGAGACTGCAATTAAAAAACTGCCCTCATCAATTGAACGGCTACAGGGGCTTAATGTATTAAGTATGAGAAACTGCACGAGCCTTGTCTCTCTTCCAGACAGCATCTGTAGTTTGGCACACCTTACATTTCTCAATCTGTCTGGGTGCTCAAAACTTTCCGACTTTCCTTACAGCTTGAGGAATACACAATCATTGACAATCAGTGGTTTAGAAGAACTCACATTCTTTATGAGAAACAAAGAAATAGAAAGTGAGATTTCATCAGACGAGTTCATCAGTCTCTCTGAAAATTCATCAGAGTCGGGGGAAGAGGTTGATGGAAAAGAAGAGAGAGTGGAGATGGAGGGGGTAGTGCAAAGGGATGATTGCTCCACGGTAGGAGGGATATTGGGGGCCTTATACTGTTTCTTGAGTTGGCTGCATGGACTATATGTTCGAGGAAAAGGGAGACTGGGTCGCCGGATTCACGGGATTGTCCGGGAATCGTTACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

1801

Amino Acids

203.91

Weight (kDa)

6.23

Isoelectric Point (pI)

39.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 20 - 198 3.6e-46 TIR domain
TIR_2 PF13676 22 - 126 3.2e-11 TIR domain
NB-ARC PF00931 203 - 362 6.1e-18 NB-ARC domain
WHD_ROQ1 PF23282 433 - 503 4.6e-12 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 702 - 840 9.1e-14 Leucine-rich repeat region
LRR_13 PF23286 712 - 796 6.2e-12 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 846 - 961 2.6e-08 Leucine-rich repeat region
LRR_13 PF23286 854 - 940 5.1e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 949 - 1117 1e-09 Leucine-rich repeat region
LRR_13 PF23286 976 - 1063 6.9e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
NB-ARC PF00931 1110 - 1274 3.1e-16 NB-ARC domain
WHD_ROQ1 PF23282 1338 - 1407 1.3e-13 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 1598 - 1703 2.1e-10 Leucine-rich repeat region
LRR_13 PF23286 1604 - 1691 2.2e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 759
AccB7I CCANNNNNTGG 1 cut(s) 1246
AccBSI CCGCTC 1 cut(s) 3869
AccI GTMKAC 5 cut(s) 1023, 2175, 3931, 4353, 4545
AccII CGCG 1 cut(s) 215
AccIII TCCGGA 3 cut(s) 2614, 2980, 4864
AciI CCGC 7 cut(s) 439, 490, 1888, 2684, 3246, 3867, 4246
AclWI GGATC 5 cut(s) 341, 1680, 3234, 3330, 3722
AcoI YGGCCR 2 cut(s) 109, 2205
AcsI RAATTY 6 cut(s) 1013, 1362, 2180, 2985, 4080, 5200
AcuI CTGAAG 7 cut(s) 1066, 1943, 2588, 3787, 3802, 3807, 4622
AcyI GRCGYC 1 cut(s) 1973
AdeI CACNNNGTG 3 cut(s) 1127, 3863, 3970
AfiI CCNNNNNNNGG 4 cut(s) 432, 1246, 3839, 3840
AflII CTTAAG 8 cut(s) 847, 2063, 2105, 2141, 3068, 4643, 4759, 4820
AflIII ACRYGT 2 cut(s) 213, 4438
AjnI CCWGG 4 cut(s) 413, 1245, 4237, 4266
AleI CACNNNNGTG 1 cut(s) 4092
AloI GAACNNNNNNTCC 4 cut(s) 3575, 3607, 5328, 5360
Alw21I GWGCWC 3 cut(s) 408, 1423, 5060
AlwI GGATC 5 cut(s) 341, 1680, 3234, 3330, 3722
AlwNI CAGNNNCTG 4 cut(s) 11, 1246, 2127, 5016
Ama87I CYCGRG 2 cut(s) 188, 3835
Aor13HI TCCGGA 3 cut(s) 2614, 2980, 4864
AoxI GGCC 8 cut(s) 109, 1000, 1482, 1989, 2205, 4000, 4096, 5304
ApoI RAATTY 6 cut(s) 1013, 1362, 2180, 2985, 4080, 5200
ArsI GACNNNNNNTTYG 2 cut(s) 5328, 5360
AseI ATTAAT 3 cut(s) 474, 2432, 4028
Asp700I GAANNNNTTC 5 cut(s) 598, 810, 2469, 2976, 3534
AspS9I GGNCC 3 cut(s) 420, 1000, 5304
AsuC2I CCSGG 2 cut(s) 1032, 5390
AsuHPI GGTGA 5 cut(s) 734, 800, 2036, 3279, 4520
AsuII TTCGAA 1 cut(s) 2597
AvaI CYCGRG 2 cut(s) 188, 3835
AvaII GGWCC 1 cut(s) 420
BalI TGGCCA 1 cut(s) 111
BanII GRGCYC 1 cut(s) 1423
BarI GAAGNNNNNNTAC 2 cut(s) 1919, 1951
BauI CACGAG 2 cut(s) 1790, 4987
BbsI GAAGAC 4 cut(s) 58, 698, 2534, 2744
Bbv12I GWGCWC 3 cut(s) 408, 1423, 5060
BbvI GCAGC 9 cut(s) 17, 853, 2133, 2150, 2343, 2772, 2925, 4829, 5316
BceAI ACGGC 5 cut(s) 2234, 3080, 4432, 4683, 4964
BciT130I CCWGG 4 cut(s) 415, 1247, 4239, 4268
BciVI GTATCC 2 cut(s) 1433, 1561
BclI TGATCA 3 cut(s) 739, 922, 3361
BcnI CCSGG 2 cut(s) 1032, 5390
BfaI CTAG 9 cut(s) 705, 1286, 1313, 1884, 2547, 2879, 3614, 3632, 3729
BfmI CTRYAG 7 cut(s) 1295, 3100, 3429, 4108, 4190, 4952, 5017
BfrI CTTAAG 8 cut(s) 847, 2063, 2105, 2141, 3068, 4643, 4759, 4820
BfuI GTATCC 2 cut(s) 1433, 1561
BglII AGATCT 3 cut(s) 99, 3892, 4136
BmcAI AGTACT 2 cut(s) 1308, 4607
Bme1390I CCNGG 6 cut(s) 415, 1032, 1247, 4239, 4268, 5390
Bme18I GGWCC 1 cut(s) 420
BmeT110I CYCGRG 2 cut(s) 188, 3835
BmgT120I GGNCC 3 cut(s) 420, 1000, 5304
BmiI GGNNCC 9 cut(s) 327, 421, 422, 1177, 1912, 3592, 4420, 4591, 5305
BmrFI CCNGG 6 cut(s) 415, 1032, 1247, 4239, 4268, 5390
BmrI ACTGGG 1 cut(s) 5372
BmsI GCATC 6 cut(s) 922, 1808, 1899, 3937, 4732, 5022
BmuI ACTGGG 1 cut(s) 5372
BpiI GAAGAC 4 cut(s) 58, 698, 2534, 2744
BplI GAGNNNNNCTC 4 cut(s) 1167, 1199, 1939, 1971
BpmI CTGGAG 2 cut(s) 1388, 4275
Bpu14I TTCGAA 1 cut(s) 2597
BpuMI CCSGG 2 cut(s) 1032, 5390
BsaAI YACGTR 1 cut(s) 1202
BsaBI GATNNNNATC 1 cut(s) 1047
BsaHI GRCGYC 1 cut(s) 1973
BsaI GGTCTC 1 cut(s) 3161
BsaJI CCNNGG 7 cut(s) 414, 1246, 1485, 2216, 3219, 4267, 5283
BsaWI WCCGGW 3 cut(s) 2614, 2980, 4864
BsaXI ACNNNNNCTCC 4 cut(s) 3575, 3605, 5350, 5380
Bsc4I CCNNNNNNNGG 4 cut(s) 432, 1246, 3839, 3840
Bse118I RCCGGY 1 cut(s) 464
Bse1I ACTGG 4 cut(s) 279, 1403, 1441, 5367
Bse3DI GCAATG 3 cut(s) 745, 3423, 4359
Bse8I GATNNNNATC 1 cut(s) 1047
BseAI TCCGGA 3 cut(s) 2614, 2980, 4864
BseBI CCWGG 4 cut(s) 415, 1247, 4239, 4268
BseDI CCNNGG 7 cut(s) 414, 1246, 1485, 2216, 3219, 4267, 5283
BseJI GATNNNNATC 1 cut(s) 1047
BseLI CCNNNNNNNGG 4 cut(s) 432, 1246, 3839, 3840
BseMI GCAATG 3 cut(s) 745, 3423, 4359
BseMII CTCAG 9 cut(s) 234, 1528, 1977, 2178, 2637, 2797, 2950, 3180, 4649
BseNI ACTGG 4 cut(s) 279, 1403, 1441, 5367
BseRI GAGGAG 2 cut(s) 1559, 3444
BseXI GCAGC 9 cut(s) 17, 853, 2133, 2150, 2343, 2772, 2925, 4829, 5316
BseYI CCCAGC 2 cut(s) 480, 3771
BsgI GTGCAG 5 cut(s) 3534, 3584, 4070, 4828, 4969
Bsh1236I CGCG 1 cut(s) 215
BshFI GGCC 8 cut(s) 111, 1002, 1484, 1991, 2207, 4002, 4098, 5306
BsiHKAI GWGCWC 3 cut(s) 408, 1423, 5060
BsiHKCI CYCGRG 2 cut(s) 188, 3835
BsiSI CCGG 8 cut(s) 350, 465, 1031, 2615, 2981, 4865, 5371, 5389
BslFI GGGAC 7 cut(s) 194, 433, 2335, 2917, 3753, 4175, 4180
BslI CCNNNNNNNGG 4 cut(s) 432, 1246, 3839, 3840
BsmBI CGTCTC 1 cut(s) 1964
BsmFI GGGAC 7 cut(s) 194, 433, 2335, 2917, 3753, 4175, 4180
BsmI GAATGC 4 cut(s) 45, 302, 804, 2553
BsnI GGCC 8 cut(s) 111, 1002, 1484, 1991, 2207, 4002, 4098, 5306
Bso31I GGTCTC 1 cut(s) 3161
BsoBI CYCGRG 2 cut(s) 188, 3835
Bsp119I TTCGAA 1 cut(s) 2597
Bsp1286I GDGCHC 3 cut(s) 408, 1423, 5060
Bsp13I TCCGGA 3 cut(s) 2614, 2980, 4864
Bsp1407I TGTACA 2 cut(s) 2024, 4703
BspACI CCGC 7 cut(s) 439, 490, 1888, 2684, 3246, 3867, 4246
BspANI GGCC 8 cut(s) 111, 1002, 1484, 1991, 2207, 4002, 4098, 5306
BspCNI CTCAG 9 cut(s) 235, 1527, 1978, 2179, 2638, 2796, 2949, 3179, 4648
BspEI TCCGGA 3 cut(s) 2614, 2980, 4864
BspFNI CGCG 1 cut(s) 215
BspHI TCATGA 1 cut(s) 4468
BspLI GGNNCC 9 cut(s) 327, 421, 422, 1177, 1912, 3592, 4420, 4591, 5305
BspMAI CTGCAG 1 cut(s) 3104
BspPI GGATC 5 cut(s) 341, 1680, 3234, 3330, 3722
BspT104I TTCGAA 1 cut(s) 2597
BspTI CTTAAG 8 cut(s) 847, 2063, 2105, 2141, 3068, 4643, 4759, 4820
BspTNI GGTCTC 1 cut(s) 3161
BsrBI CCGCTC 1 cut(s) 3869
BsrDI GCAATG 3 cut(s) 745, 3423, 4359
BsrFI RCCGGY 1 cut(s) 464
BsrGI TGTACA 2 cut(s) 2024, 4703
BsrI ACTGG 4 cut(s) 279, 1403, 1441, 5367
BssAI RCCGGY 1 cut(s) 464
BssECI CCNNGG 7 cut(s) 414, 1246, 1485, 2216, 3219, 4267, 5283
BssNAI GTATAC 3 cut(s) 1024, 3932, 4354
BssNI GRCGYC 1 cut(s) 1973
BssSI CACGAG 2 cut(s) 1790, 4987
BssT1I CCWWGG 2 cut(s) 1485, 3219
Bst1107I GTATAC 3 cut(s) 1024, 3932, 4354
Bst2BI CACGAG 2 cut(s) 1790, 4987
Bst2UI CCWGG 4 cut(s) 415, 1247, 4239, 4268
Bst6I CTCTTC 4 cut(s) 2460, 5007, 5214, 5232
BstACI GRCGYC 1 cut(s) 1973
BstAFI CTTAAG 8 cut(s) 847, 2063, 2105, 2141, 3068, 4643, 4759, 4820
BstAPI GCANNNNNTGC 1 cut(s) 11
BstAUI TGTACA 2 cut(s) 2024, 4703
BstBAI YACGTR 1 cut(s) 1202
BstBI TTCGAA 1 cut(s) 2597
BstC8I GCNNGC 4 cut(s) 466, 1241, 2708, 3864
BstDSI CCRYGG 2 cut(s) 2216, 5283
BstEII GGTNACC 1 cut(s) 4262
BstFNI CGCG 1 cut(s) 215
BstNI CCWGG 4 cut(s) 415, 1247, 4239, 4268
BstNSI RCATGY 5 cut(s) 1326, 1879, 3878, 4442, 4558
BstPI GGTNACC 1 cut(s) 4262
BstSCI CCNGG 6 cut(s) 413, 1030, 1245, 4237, 4266, 5388
BstSFI CTRYAG 7 cut(s) 1295, 3100, 3429, 4108, 4190, 4952, 5017
BstUI CGCG 1 cut(s) 215
BstV1I GCAGC 9 cut(s) 17, 853, 2133, 2150, 2343, 2772, 2925, 4829, 5316
BstV2I GAAGAC 4 cut(s) 58, 698, 2534, 2744
BstX2I RGATCY 5 cut(s) 99, 346, 3226, 3892, 4136
BstXI CCANNNNNNTGG 2 cut(s) 3202, 4734
BstYI RGATCY 5 cut(s) 99, 346, 3226, 3892, 4136
BstZ17I GTATAC 3 cut(s) 1024, 3932, 4354
BsuI GTATCC 2 cut(s) 1433, 1561
BsuRI GGCC 8 cut(s) 111, 1002, 1484, 1991, 2207, 4002, 4098, 5306
BtgI CCRYGG 2 cut(s) 2216, 5283
BtgZI GCGATG 1 cut(s) 685
BtsIMutI CAGTG 3 cut(s) 1434, 3469, 5119
Cac8I GCNNGC 4 cut(s) 466, 1241, 2708, 3864
CaiI CAGNNNCTG 4 cut(s) 11, 1246, 2127, 5016
CciI TCATGA 1 cut(s) 4468
Cfr10I RCCGGY 1 cut(s) 464
Cfr13I GGNCC 3 cut(s) 420, 1000, 5304
CseI GACGC 3 cut(s) 204, 221, 1981
CspCI CAANNNNNGTGG 4 cut(s) 1981, 2016, 2388, 2423
DraI TTTAAA 3 cut(s) 543, 1333, 3757
DraIII CACNNNGTG 3 cut(s) 1127, 3863, 3970
EaeI YGGCCR 2 cut(s) 109, 2205
Eam1104I CTCTTC 4 cut(s) 2460, 5007, 5214, 5232
EarI CTCTTC 4 cut(s) 2460, 5007, 5214, 5232
Ecl136II GAGCTC 1 cut(s) 1421
Eco130I CCWWGG 2 cut(s) 1485, 3219
Eco24I GRGCYC 1 cut(s) 1423
Eco31I GGTCTC 1 cut(s) 3161
Eco32I GATATC 1 cut(s) 652
Eco47I GGWCC 1 cut(s) 420
Eco53kI GAGCTC 1 cut(s) 1421
Eco57I CTGAAG 7 cut(s) 1066, 1943, 2588, 3787, 3802, 3807, 4622
Eco88I CYCGRG 2 cut(s) 188, 3835
Eco91I GGTNACC 1 cut(s) 4262
EcoICRI GAGCTC 1 cut(s) 1421
EcoO109I RGGNCCY 3 cut(s) 420, 1000, 5304
EcoO65I GGTNACC 1 cut(s) 4262
EcoRII CCWGG 4 cut(s) 413, 1245, 4237, 4266
EcoRV GATATC 1 cut(s) 652
EcoT14I CCWWGG 2 cut(s) 1485, 3219
EcoT22I ATGCAT 2 cut(s) 302, 1464
EcoT38I GRGCYC 1 cut(s) 1423
ErhI CCWWGG 2 cut(s) 1485, 3219
Esp3I CGTCTC 1 cut(s) 1964
FalI AAGNNNNNCTT 4 cut(s) 1064, 1096, 1998, 2030
FaqI GGGAC 7 cut(s) 194, 433, 2335, 2917, 3753, 4175, 4180
FauI CCCGC 1 cut(s) 497
FauNDI CATATG 2 cut(s) 2559, 4809
FbaI TGATCA 3 cut(s) 739, 922, 3361
FblI GTMKAC 5 cut(s) 1023, 2175, 3931, 4353, 4545
FriOI GRGCYC 1 cut(s) 1423
FspBI CTAG 9 cut(s) 705, 1286, 1313, 1884, 2547, 2879, 3614, 3632, 3729
GsaI CCCAGC 2 cut(s) 484, 3775
GsuI CTGGAG 2 cut(s) 1388, 4275
HaeIII GGCC 8 cut(s) 111, 1002, 1484, 1991, 2207, 4002, 4098, 5306
HapII CCGG 8 cut(s) 350, 465, 1031, 2615, 2981, 4865, 5371, 5389
HgaI GACGC 3 cut(s) 204, 221, 1981
Hin1I GRCGYC 1 cut(s) 1973
HincII GTYRAC 9 cut(s) 1455, 1524, 1554, 1867, 2176, 2342, 2924, 4287, 4546
HindII GTYRAC 9 cut(s) 1455, 1524, 1554, 1867, 2176, 2342, 2924, 4287, 4546
HindIII AAGCTT 5 cut(s) 221, 512, 1635, 1916, 4595
HpaI GTTAAC 1 cut(s) 1554
HpaII CCGG 8 cut(s) 350, 465, 1031, 2615, 2981, 4865, 5371, 5389
HphI GGTGA 5 cut(s) 734, 800, 2036, 3279, 4520
Hpy99I CGWCG 1 cut(s) 1503
HpyCH4IV ACGT 3 cut(s) 183, 1201, 2456
HpySE526I ACGT 3 cut(s) 183, 1201, 2456
Hsp92I GRCGYC 1 cut(s) 1973
KflI GGGWCCC 1 cut(s) 420
Kpn2I TCCGGA 3 cut(s) 2614, 2980, 4864
KroI GCCGGC 1 cut(s) 464
KroNI GCCGGC 1 cut(s) 466
Ksp22I TGATCA 3 cut(s) 739, 922, 3361
KspAI GTTAAC 1 cut(s) 1554
LmnI GCTCC 8 cut(s) 1175, 3457, 3596, 3908, 4404, 4424, 4888, 5285
Lsp1109I GCAGC 9 cut(s) 17, 853, 2133, 2150, 2343, 2772, 2925, 4829, 5316
LweI GCATC 6 cut(s) 922, 1808, 1899, 3937, 4732, 5022
MaeI CTAG 9 cut(s) 705, 1286, 1313, 1884, 2547, 2879, 3614, 3632, 3729
MaeII ACGT 3 cut(s) 183, 1201, 2456
MbiI CCGCTC 1 cut(s) 3869
MfeI CAATTG 6 cut(s) 2670, 3057, 3304, 3825, 4090, 4941
MflI RGATCY 5 cut(s) 99, 346, 3226, 3892, 4136
MhlI GDGCHC 3 cut(s) 408, 1423, 5060
MlsI TGGCCA 1 cut(s) 111
MluI ACGCGT 1 cut(s) 213
MluNI TGGCCA 1 cut(s) 111
MlyI GAGTC 8 cut(s) 1957, 2092, 2329, 2521, 2548, 2758, 2911, 5219
MmeI TCCRAC 6 cut(s) 571, 643, 3367, 4426, 4820, 5094
Mox20I TGGCCA 1 cut(s) 111
Mph1103I ATGCAT 2 cut(s) 302, 1464
MroI TCCGGA 3 cut(s) 2614, 2980, 4864
MroNI GCCGGC 1 cut(s) 464
MroXI GAANNNNTTC 5 cut(s) 598, 810, 2469, 2976, 3534
MscI TGGCCA 1 cut(s) 111
MslI CAYNNNNRTG 8 cut(s) 2448, 2558, 2658, 3024, 3996, 4092, 4224, 4808
Msp20I TGGCCA 1 cut(s) 111
MspA1I CMGCKG 4 cut(s) 8, 2686, 2710, 3434
MspCI CTTAAG 8 cut(s) 847, 2063, 2105, 2141, 3068, 4643, 4759, 4820
MspI CCGG 8 cut(s) 350, 465, 1031, 2615, 2981, 4865, 5371, 5389
MspR9I CCNGG 6 cut(s) 415, 1032, 1247, 4239, 4268, 5390
MunI CAATTG 6 cut(s) 2670, 3057, 3304, 3825, 4090, 4941
Mva1269I GAATGC 4 cut(s) 45, 302, 804, 2553
MvaI CCWGG 4 cut(s) 415, 1247, 4239, 4268
MvnI CGCG 1 cut(s) 215
NaeI GCCGGC 1 cut(s) 466
NciI CCSGG 2 cut(s) 1032, 5390
NdeI CATATG 2 cut(s) 2559, 4809
NgoMIV GCCGGC 1 cut(s) 464
NlaIV GGNNCC 9 cut(s) 327, 421, 422, 1177, 1912, 3592, 4420, 4591, 5305
NmeAIII GCCGAG 2 cut(s) 2233, 2934
NmuCI GTSAC 5 cut(s) 1426, 2284, 3285, 3855, 4628
NsiI ATGCAT 2 cut(s) 302, 1464
NspI RCATGY 5 cut(s) 1326, 1879, 3878, 4442, 4558
NspV TTCGAA 1 cut(s) 2597
OliI CACNNNNGTG 1 cut(s) 4092
PaeR7I CTCGAG 1 cut(s) 3835
PagI TCATGA 1 cut(s) 4468
PciI ACATGT 1 cut(s) 4438
PcsI WCGNNNNNNNCGW 1 cut(s) 288
PctI GAATGC 4 cut(s) 45, 302, 804, 2553
PdiI GCCGGC 1 cut(s) 466
PdmI GAANNNNTTC 5 cut(s) 598, 810, 2469, 2976, 3534
PflFI GACNNNGTC 1 cut(s) 5364
PflMI CCANNNNNTGG 1 cut(s) 1246
PfoI TCCNGGA 1 cut(s) 5388
PleI GAGTC 8 cut(s) 1956, 2091, 2328, 2520, 2547, 2757, 2910, 5218
PpsI GAGTC 8 cut(s) 1956, 2091, 2328, 2520, 2547, 2757, 2910, 5218
Ppu21I YACGTR 1 cut(s) 1202
PpuMI RGGWCCY 1 cut(s) 420
PscI ACATGT 1 cut(s) 4438
PshBI ATTAAT 3 cut(s) 474, 2432, 4028
PsiI TTATAA 1 cut(s) 759
Psp124BI GAGCTC 1 cut(s) 1423
Psp5II RGGWCCY 1 cut(s) 420
Psp6I CCWGG 4 cut(s) 413, 1245, 4237, 4266
PspEI GGTNACC 1 cut(s) 4262
PspFI CCCAGC 2 cut(s) 480, 3771
PspGI CCWGG 4 cut(s) 413, 1245, 4237, 4266
PspN4I GGNNCC 9 cut(s) 327, 421, 422, 1177, 1912, 3592, 4420, 4591, 5305
PspPI GGNCC 3 cut(s) 420, 1000, 5304
PspPPI RGGWCCY 1 cut(s) 420
PsrI GAACNNNNNNTAC 2 cut(s) 1389, 1421
PstI CTGCAG 1 cut(s) 3104
PstNI CAGNNNCTG 4 cut(s) 11, 1246, 2127, 5016
PsuI RGATCY 5 cut(s) 99, 346, 3226, 3892, 4136
PsyI GACNNNGTC 1 cut(s) 5364
PvuII CAGCTG 3 cut(s) 8, 2710, 3434
RseI CAYNNNNRTG 8 cut(s) 2448, 2558, 2658, 3024, 3996, 4092, 4224, 4808
SacI GAGCTC 1 cut(s) 1423
SalI GTCGAC 2 cut(s) 2174, 4544
Sau96I GGNCC 3 cut(s) 420, 1000, 5304
ScaI AGTACT 2 cut(s) 1308, 4607
SchI GAGTC 8 cut(s) 1957, 2092, 2329, 2521, 2548, 2758, 2911, 5219
ScrFI CCNGG 6 cut(s) 415, 1032, 1247, 4239, 4268, 5390
SduI GDGCHC 3 cut(s) 408, 1423, 5060
SfaNI GCATC 6 cut(s) 922, 1808, 1899, 3937, 4732, 5022
SfcI CTRYAG 7 cut(s) 1295, 3100, 3429, 4108, 4190, 4952, 5017
Sfr274I CTCGAG 1 cut(s) 3835
SfuI TTCGAA 1 cut(s) 2597
SinI GGWCC 1 cut(s) 420
SlaI CTCGAG 1 cut(s) 3835
SmiMI CAYNNNNRTG 8 cut(s) 2448, 2558, 2658, 3024, 3996, 4092, 4224, 4808
SsiI CCGC 7 cut(s) 439, 490, 1888, 2684, 3246, 3867, 4246
SspMI CTAG 9 cut(s) 705, 1286, 1313, 1884, 2547, 2879, 3614, 3632, 3729
SstI GAGCTC 1 cut(s) 1423
StyD4I CCNGG 6 cut(s) 413, 1030, 1245, 4237, 4266, 5388
StyI CCWWGG 2 cut(s) 1485, 3219
TaiI ACGT 3 cut(s) 186, 1204, 2459
TatI WGTACW 6 cut(s) 1261, 1306, 2024, 3235, 4605, 4703
TauI GCSGC 3 cut(s) 1890, 2686, 3869
TscAI CASTG 3 cut(s) 1441, 3476, 5119
TseFI GTSAC 5 cut(s) 1426, 2284, 3285, 3855, 4628
Tsp45I GTSAC 5 cut(s) 1426, 2284, 3285, 3855, 4628
TspGWI ACGGA 3 cut(s) 448, 1487, 3971
TspRI CASTG 3 cut(s) 1441, 3476, 5119
Tth111I GACNNNGTC 1 cut(s) 5364
Van91I CCANNNNNTGG 1 cut(s) 1246
Vha464I CTTAAG 8 cut(s) 847, 2063, 2105, 2141, 3068, 4643, 4759, 4820
VpaK11BI GGWCC 1 cut(s) 420
VspI ATTAAT 3 cut(s) 474, 2432, 4028
XapI RAATTY 6 cut(s) 1013, 1362, 2180, 2985, 4080, 5200
XbaI TCTAGA 1 cut(s) 1312
XceI RCATGY 5 cut(s) 1326, 1879, 3878, 4442, 4558
XcmI CCANNNNNNNNNTGG 2 cut(s) 583, 862
XhoI CTCGAG 1 cut(s) 3835
XmiI GTMKAC 5 cut(s) 1023, 2175, 3931, 4353, 4545
XmnI GAANNNNTTC 5 cut(s) 598, 810, 2469, 2976, 3534
XspI CTAG 9 cut(s) 705, 1286, 1313, 1884, 2547, 2879, 3614, 3632, 3729
ZrmI AGTACT 2 cut(s) 1308, 4607
Zsp2I ATGCAT 2 cut(s) 302, 1464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.