RLG00000026639

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0010532
Physical Location & Seq
Reverse (-)
4508176 .. 4515376
7201 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026639

Sequence Viewer

Length: 5340 bp
ATGGCTGCTGCTGCTGATTCTTCTTCTTCTCGCATTCGTGGCAGTTATGATGTGTTCTTGAGTTTTAGAGGGTTTGACACTCGCAAGATCTTTGTTGGCCATCTTTACAATGCTCTACAAAGGAAAGCACTCGCTACCTTCATAGATACTGAAGAGCTTGGAAAAGGGGACGTTCTCGGGGTGCTACTGAAAGCTATTGACGCGTCAAAGCTTTCGGTGGTAGTTTTATCTGAGAACTTTGCCTATTCCAAATGGTGCTTACTGGAACTCGAAAAAATCGTGGAATGCATGGAAACAAAGAATCATATAGTGGTTCCCATTTTCTACCAAGTAGATCCGGCTGATGTTCGTTATGTGAGGGGAAGTTTCAGCAAAGCGTTTGAACAACATGAGCACAAGTCCAGGGGGACCGCTGAAGAACGGAAGCGGTGGAAGTCTGCTCTAATTAGAGCCGGCTATATTAATGGCTGGGATTCCCGCAAATACGAGGATGATGTCAAGCTTATTGATGATGTTGTTAAAAATGTTTTAAAACAGTTGAGCAACATCAGGGAAAGTAAATCCAATGATGACTTGGTTGGAATGGATTCCCACATAAGCAACATGAAGTCTCTGTTAAGTGCTTATGGGTCGGGGGATGTCTGCATTGTTGGAATATGGGGTATGGGTGGTATAGGGAAGACAACCATCGCTAGAGCTGTTTATGAGGAAATGTGTGGAAACTTTGATCACCATTGCTTTCTTTATAATGTCAAGGAAGATTTCAAGAGAAAAGGTGAAGCAGGAATGCAGGAAGAACTTCTATATAGAATCTCAAGGGAAAATGGGCAGCACTTAAGTTCCAAAATGATTATGGAAAGGCTTAGTGGGATAAAACTTTTTCTTGTTCTTGATGATGTGGACAGTTCTGATCAAATTGATGCCCTACTTGGAAAACAATGTTCATTTGGTCGTGGAAGTAGAATCATCATAACGACCAGAGATAAGGGCCTACTTAGAGAATTTGATGTATATGAACCGGGATATTTAGATGAAGATCAAGCTCTTGAACTCTTTAAGAAGTATGCCTTCAGAACAATCGAACCCTTAACAGAGTACGATCATCTCTCACAGTGTGCCATAAAATATGCTCAAGGTCTGCCTTTAGCACTTAAAGTCTTGGGAGCCTCTCTCTATAAGAAAAGTGTCCGTGAGTGGGAAGTTGAGTTTGAAAAAATAAAGAAATGCCAGCACCTGGGAATTGAGGGTGTTCTAAGGACAACCTTCGAAGGACTAGATCATCTACAGAAAACAGTACTTCTAGATATTGCATGTTTCTTTAGAGAAATGAAAAAGGACTATGCAACAGAAATTCTGGAGGCCTGTGGCTTCTTTCCCATTAGTGTATTACCAGTTCTAGTTGATAGAGCTCTTGTGACTAGCACTGGATACTATGTTGTGTTGACAATGCATGATTTACTATTGGACATGGCCTTGGGAATTGTCCGTCGAGAATCAAGCCTCAGTAGGTTGACGAGTGCTGATGAAATCGAGGAGGTGTTAACTCAAAACACGGATACGAAAGCAGTTGAAAGCATAGACCTCGATTTGTACCACTCAAAAGACATACGCATAAATGCTAAAGCTTTTGTTCGCATGACGAAACTAAGACTGCTTAGAATCCGATATAACCGCTCCATAGACTTTGTTGATGATTATTATGGTTCACGGGGGGAATTATACCATCTAATTGATGACTGTAAACAACAAGTGAGTGGGGAGTTAAAGTTCCTTTCTCACGAATTGAGGTGTCTCATCTGGCATGGATGTCCCCTGAAATCTTTGCCATCCAATTTCAACCCAAAGCATCTAGTTGACCTTGACATGCGTTCTAGCCGCATCAGACAACTTTGGAAAGGAACCAAGCTTCTGAAGAAGTTGAAATACATTGATTTGAGTCTATCTCTATACCTTAAGGAGACGCCCGACTTAACTGAGGCCACAAATCTTGAGAAAGTAAACCTTGAAGGTTGTACAAGTTTATTGGAGGTTCACCCATCCATCTCTGATCTTAAGAACCTTGTTTTCTTGAGTCTAAAAGGGTGCAAAGAGCTTAAGATTCTTCCAAGCAGCTTCTGTATGAAATCTCTTAAGACCCTTGAGCTTTCTGGCTGCTCAAATCTCGACAAATTTCCTGAGATTTCAGAAGTTATGGCGGAGCTACCACGGCTTTGTTTAGATAAGACTGCAATTAAAGAACTGCCCTCATCAATAAAAAATCTTAGGGGGCTTGTCACTTTGAGCCTGAAAGATTGCAGAGAACTTGAGAGTCTCCCAAGCAGCATTTGTCAACTCAAGTCTCTCAGATATGTTATACTCTCTGGGTGTTCCAAGTTTAAGGTGTTTCCAAAAATTATGGGGGTAGAAGATATGAAAGGATTAATAGAGCTTCATATGGATGGAACGTCTATCGAAGAGTTTCTGCCGTCAATTTCAGCTCTTAAAGAACTTGTTGTCTTGAGTCTAAGAGATTGCAAACAGCTAAAGAGTCTTCCTAGTAGCATTCATATGAAATCTCTTGACACACTTATTCTTTCTGGATGTTCGAACCTTCAGAAGTTTCCAGAGATTTCAGAAAAAATGTGGAGGCTCTCAGAGCTTCATTTAGATGATATTGCAATTGAAGAACTGCCGCTGTCAATTAAAAATCTTTGCCAGCTGGAATCTTTGAGCCTTAAAGATTGTGGAGAACTCAAGAGTCTTCCAAGCAGCATTTTTGAACTTGAGAGCCTCAGTATGATTACTGTCTCTGGTTGTTCAAAGTTTAAGGTGTTTCCAAAAAATGAAGAAGATTTGGAAGGACTACAAAAACTTGTTACATTGAGGTTAGAAGATTGCGGAGAACTTAGGAGTCTGCCGAGCAGCATTTGTCAACTCAAGTCCCTCAGATTTGTTGCTCTCTCTGGTTGTTCAAAGTTTGAAGTGTTTCCGGAAATTTTAGAAGCTATGGAAGAATTAAGAGAGCTTCATTTGGATGGAACATCTGTCAAAGACCTTTCCCCATCAATTGAACGGCTTAAGAGGATTATGTTATTGAACATGAGAAACTGCAGAAGCCTCATCTTTCTTCCTGAAAATATCTGTAATTTGTCATACCTTACAGGTCTCACTCTCTCAGGGTGCTTGAATCTCCATAACTTGCCCAAGAACTTGGGGAAATTAGAATCCTTGCTGGATCTTGAAGTACAGGGAAGTGGTATAGAGCAACCACTCTCTCTCTTGCATACAAAGAAGTCACCATCATCATCAGCCAATTGCTTAGTTGGCATGGATCGCCATATAAATAAAATGCAATCGCTATTATGTCTTGATCAGGTGGATGATGTTCGTATTGTTGGAATATGTGGAATTGGGGGTATCGGCAAGACAACCATTGCTACAGCTGTATATGATAAAATTGCTCCTCAATTTGAACACCATTGTTTTCTTGAGAATGTCAAGGAAGGTTTCACAAAGCATAGTGCAGAACAAATGCAGGAAGAACTTCTGACCAGAATCTTAAAGGTAAAGGTGCAGAGTTTAAGCATTTTGAACAGAGGCTCCAATATGCTTATTGAAAGGCTAGGTACGAAAAAAGTTCTAGTTGTTCTTGATGATATAGACGATATAACCCAGATTGAAACCTTGCTTGAAAAGCCATATTCATTTGGTGGTGGGAGTAGGATCATTGTAACAACTAGAAAGGAAGAAATAATGAGTGGATTTAAAATATATCGTCCCCAGCTATCAAATGCTGAAGCTGTTGAAATCTTCAGACATTTTGCCTTCAGAACAATTGAACCCTCGAGAGGGTATGATGATCTGTCACAGCGTGCCGCACAACATGCTCAAGTGCCTTTAGATCTTAAAGTCTGGGGAGCTTTTCTTTTCAACAAAAGTATGCATGAGTGGGAAGATGCCTTGACGGATAAGCACACAGTGTATTGGGTGCTAACAACACACTTTTATGGCCTACATTACTCGGAGAGGAACATATTAATAGATGTTGCATGGTTCTTTGAAGGAATGAAAAAAGAGTATGCAACAAAAATTCTGCACAATTGTGGCCTTTTTCCCTATAGTGCATTAAGAGGTCTAATTGATAGATCTCTCGTATCTATCTCAGAAAGGGACGGGACTATCAAACTGCATGATTTACTACAGGGGATTGTTCGGGATTGTGTTCGCCACGAAGATGAACATGAACCTGGGAACCGCAGTAGATTAGAGGGTTACCAGGGAATAAGGTGTTTGTCAACTCAAGATCAGGTTACTAAAGCAGTTGAAGTTATAACCCTTGATCTGTCAAACTCAAAAGAGGTATACTTCATTGCAGAGGCTTTTGTTACAATGAAGAAATTAAGACTGCTCCGAATTTATGATAACGGCTCCATTAACTGTAAACAACATCTTCTGAAGTACTTGACGTTCATCAATTTAAGTCACTCTCAGTATCTTAAGGAAATCCCCGACTTAACCACGGCTACAAATCTTGAGAAGCTAATTCTTGATGGTTGTACAAGTTTATTCGAGGTGCATCCATCCATTTGGAATCACAAAAACCTCATTTTCTTAAGTCTAAAAGGTTGCAGACAACTTGAGATTCTTCCAACCAACATCCATATGAAATCTCTTAAGACCCTTAATCTTTCTGGCTGCACAAATCTTGAGAAGTTTCCGGAGATTTCAGAAGTTATGATGGAGCTATCAGAGCTTGGTTTAGATGAGATTGCAATTAAAAAACTGCCCTCATCAATTGAACGGCTACATGGGCTCAGTGTATTAAGTATGAGAAACTGCACGAGCCTTGTCTCTCTTCCAGACAGCATCTGTAGTTTGGCACACCTTACATTTCTCAATCTGTCTGGGTGCTCAAAACTTTCCGACTTTCCTGACAGCTTGAGGAATACACAATCATTCACAATCAGTGGTTTAGAAGAACTCACATTCTGTATGAGAAACAAAGAAATAGAAAGTGAGATTTCATCAGACGAGTTCATCAGTCTCTCTGAGAATTCATCAGAGTCGGGGGAAGAGGATGGAAAAGAAGAGAGAGTGGAGATGGAGGGGGTAGTGCAAAGGGATGATTGCTCCACGGTAGGAAAGATGTTGGGGGCCTTATACTGCTTCTTGAGTTGGATGCATGGACTATATGTTCGAGGAAAAGGGCGACTGGGTCTTCACACTTTGCAAACAACTAATGCACCGAATCGTCTCACTGAACACCCATGTCGGTGCTCCTCCCGCTGCCTTGAGGAGTCAATTGCAGCTATTTTTTTGTTCATTGATTGCAAATTAACAGATGAATGGCTTGCAGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

1780

Amino Acids

201.27

Weight (kDa)

6.14

Isoelectric Point (pI)

40.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 16 - 194 1.5e-46 TIR domain
TIR_2 PF13676 18 - 122 2.3e-11 TIR domain
NB-ARC PF00931 199 - 358 5.3e-18 NB-ARC domain
WHD_ROQ1 PF23282 429 - 499 1.2e-11 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 699 - 840 1.9e-13 Leucine-rich repeat region
LRR_13 PF23286 708 - 792 4.7e-12 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 842 - 957 1.6e-08 Leucine-rich repeat region
LRR_13 PF23286 850 - 936 7e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 953 - 1113 1.5e-08 Leucine-rich repeat region
LRR_13 PF23286 972 - 1058 7.3e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
NB-ARC PF00931 1106 - 1270 1.3e-16 NB-ARC domain
WHD_ROQ1 PF23282 1334 - 1403 1.3e-13 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 1542 - 1645 3.1e-09 Leucine-rich repeat region
LRR_13 PF23286 1548 - 1635 1.1e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 747, 4310
AccB7I CCANNNNNTGG 1 cut(s) 1234
AccBSI CCGCTC 1 cut(s) 1674
AccI GTMKAC 1 cut(s) 4341
AccII CGCG 1 cut(s) 203
AccIII TCCGGA 2 cut(s) 2968, 4696
AclWI GGATC 4 cut(s) 329, 3222, 3318, 3710
AcoI YGGCCR 1 cut(s) 97
AcsI RAATTY 7 cut(s) 1001, 1350, 2168, 2973, 4068, 4392, 5032
AcuI CTGAAG 9 cut(s) 171, 435, 1054, 1931, 2576, 3775, 3790, 3795, 4454
AcyI GRCGYC 1 cut(s) 1961
AdeI CACNNNGTG 3 cut(s) 1115, 3851, 3958
AfaI GTAC 8 cut(s) 1097, 1296, 1592, 2014, 3225, 3607, 4439, 4537
AfiI CCNNNNNNNGG 4 cut(s) 1195, 1234, 3827, 3828
AflII CTTAAG 9 cut(s) 835, 1952, 2051, 2093, 2129, 3056, 4475, 4591, 4652
AflIII ACRYGT 1 cut(s) 201
AjnI CCWGG 4 cut(s) 401, 1233, 4225, 4254
AleI CACNNNNGTG 1 cut(s) 4080
AloI GAACNNNNNNTCC 6 cut(s) 1751, 1783, 3563, 3595, 5157, 5189
Alw21I GWGCWC 4 cut(s) 396, 1411, 4892, 5258
AlwI GGATC 4 cut(s) 329, 3222, 3318, 3710
AlwNI CAGNNNCTG 3 cut(s) 1234, 2115, 4848
Ama87I CYCGRG 2 cut(s) 176, 3823
Aor13HI TCCGGA 2 cut(s) 2968, 4696
AoxI GGCC 8 cut(s) 97, 988, 1359, 1470, 1977, 3988, 4084, 5133
ApoI RAATTY 7 cut(s) 1001, 1350, 2168, 2973, 4068, 4392, 5032
ArsI GACNNNNNNTTYG 2 cut(s) 5157, 5189
AseI ATTAAT 3 cut(s) 462, 2420, 4016
Asp700I GAANNNNTTC 5 cut(s) 586, 798, 2457, 2964, 3522
AspS9I GGNCC 3 cut(s) 408, 988, 5133
AsuC2I CCSGG 1 cut(s) 1020
AsuHPI GGTGA 4 cut(s) 722, 788, 2024, 3267
AsuII TTCGAA 2 cut(s) 1266, 2585
AvaI CYCGRG 2 cut(s) 176, 3823
AvaII GGWCC 1 cut(s) 408
BalI TGGCCA 1 cut(s) 99
BanII GRGCYC 2 cut(s) 1411, 4794
BarI GAAGNNNNNNTAC 2 cut(s) 1907, 1939
BauI CACGAG 1 cut(s) 4819
BbsI GAAGAC 4 cut(s) 686, 2522, 2732, 5189
Bbv12I GWGCWC 4 cut(s) 396, 1411, 4892, 5258
BbvI GCAGC 9 cut(s) 841, 2121, 2138, 2331, 2760, 2913, 4661, 5252, 5297
BceAI ACGGC 6 cut(s) 2222, 2449, 3068, 4420, 4515, 4796
BcgI CGANNNNNNTGC 2 cut(s) 1564, 1598
BciT130I CCWGG 4 cut(s) 403, 1235, 4227, 4256
BciVI GTATCC 2 cut(s) 1421, 1549
BclI TGATCA 3 cut(s) 727, 910, 3349
BcnI CCSGG 1 cut(s) 1020
BfmI CTRYAG 6 cut(s) 1283, 3088, 3417, 4096, 4178, 4849
BfrI CTTAAG 9 cut(s) 835, 1952, 2051, 2093, 2129, 3056, 4475, 4591, 4652
BfuI GTATCC 2 cut(s) 1421, 1549
BglII AGATCT 3 cut(s) 87, 3880, 4124
BmcAI AGTACT 2 cut(s) 1296, 4439
Bme1390I CCNGG 5 cut(s) 403, 1020, 1235, 4227, 4256
Bme18I GGWCC 1 cut(s) 408
BmeT110I CYCGRG 2 cut(s) 176, 3823
BmgT120I GGNCC 3 cut(s) 408, 988, 5133
BmiI GGNNCC 8 cut(s) 315, 409, 1165, 1900, 3580, 4232, 4408, 5134
BmrFI CCNGG 5 cut(s) 403, 1020, 1235, 4227, 4256
BmrI ACTGGG 1 cut(s) 5201
BmsI GCATC 7 cut(s) 910, 1855, 1887, 3925, 4564, 4854, 5148
BmuI ACTGGG 1 cut(s) 5201
BpiI GAAGAC 4 cut(s) 686, 2522, 2732, 5189
BplI GAGNNNNNCTC 4 cut(s) 1155, 1187, 1927, 1959
BpmI CTGGAG 1 cut(s) 1376
Bpu14I TTCGAA 2 cut(s) 1266, 2585
BpuMI CCSGG 1 cut(s) 1020
BsaBI GATNNNNATC 1 cut(s) 1035
BsaHI GRCGYC 1 cut(s) 1961
BsaI GGTCTC 1 cut(s) 3149
BsaJI CCNNGG 8 cut(s) 402, 1234, 1473, 2204, 4226, 4255, 4497, 5112
BsaWI WCCGGW 2 cut(s) 2968, 4696
BsaXI ACNNNNNCTCC 4 cut(s) 1751, 1781, 3563, 3593
Bsc4I CCNNNNNNNGG 4 cut(s) 1195, 1234, 3827, 3828
Bse118I RCCGGY 1 cut(s) 452
Bse1I ACTGG 4 cut(s) 267, 1391, 1429, 5196
Bse3DI GCAATG 3 cut(s) 733, 3411, 4347
Bse8I GATNNNNATC 1 cut(s) 1035
BseAI TCCGGA 2 cut(s) 2968, 4696
BseBI CCWGG 4 cut(s) 403, 1235, 4227, 4256
BseDI CCNNGG 8 cut(s) 402, 1234, 1473, 2204, 4226, 4255, 4497, 5112
BseJI GATNNNNATC 1 cut(s) 1035
BseLI CCNNNNNNNGG 4 cut(s) 1195, 1234, 3827, 3828
BseMI GCAATG 3 cut(s) 733, 3411, 4347
BseNI ACTGG 4 cut(s) 267, 1391, 1429, 5196
BseRI GAGGAG 4 cut(s) 1547, 3432, 5248, 5288
BseXI GCAGC 9 cut(s) 841, 2121, 2138, 2331, 2760, 2913, 4661, 5252, 5297
BseYI CCCAGC 2 cut(s) 468, 3759
BsgI GTGCAG 5 cut(s) 3522, 3572, 4058, 4660, 4801
Bsh1236I CGCG 1 cut(s) 203
BshFI GGCC 8 cut(s) 99, 990, 1361, 1472, 1979, 3990, 4086, 5135
BsiHKAI GWGCWC 4 cut(s) 396, 1411, 4892, 5258
BsiHKCI CYCGRG 2 cut(s) 176, 3823
BsiSI CCGG 5 cut(s) 338, 453, 1019, 2969, 4697
BslFI GGGAC 7 cut(s) 182, 421, 1794, 2905, 3741, 4163, 4168
BslI CCNNNNNNNGG 4 cut(s) 1195, 1234, 3827, 3828
BsmBI CGTCTC 2 cut(s) 1952, 5237
BsmFI GGGAC 7 cut(s) 182, 421, 1794, 2905, 3741, 4163, 4168
BsmI GAATGC 4 cut(s) 33, 290, 792, 2541
BsnI GGCC 8 cut(s) 99, 990, 1361, 1472, 1979, 3990, 4086, 5135
Bso31I GGTCTC 1 cut(s) 3149
BsoBI CYCGRG 2 cut(s) 176, 3823
Bsp119I TTCGAA 2 cut(s) 1266, 2585
Bsp1286I GDGCHC 5 cut(s) 396, 1411, 4794, 4892, 5258
Bsp13I TCCGGA 2 cut(s) 2968, 4696
Bsp1407I TGTACA 2 cut(s) 2012, 4535
BspANI GGCC 8 cut(s) 99, 990, 1361, 1472, 1979, 3990, 4086, 5135
BspEI TCCGGA 2 cut(s) 2968, 4696
BspFNI CGCG 1 cut(s) 203
BspLI GGNNCC 8 cut(s) 315, 409, 1165, 1900, 3580, 4232, 4408, 5134
BspMAI CTGCAG 1 cut(s) 3092
BspPI GGATC 4 cut(s) 329, 3222, 3318, 3710
BspQI GCTCTTC 1 cut(s) 147
BspT104I TTCGAA 2 cut(s) 1266, 2585
BspTI CTTAAG 9 cut(s) 835, 1952, 2051, 2093, 2129, 3056, 4475, 4591, 4652
BspTNI GGTCTC 1 cut(s) 3149
BsrBI CCGCTC 1 cut(s) 1674
BsrDI GCAATG 3 cut(s) 733, 3411, 4347
BsrFI RCCGGY 1 cut(s) 452
BsrGI TGTACA 2 cut(s) 2012, 4535
BsrI ACTGG 4 cut(s) 267, 1391, 1429, 5196
BssAI RCCGGY 1 cut(s) 452
BssECI CCNNGG 8 cut(s) 402, 1234, 1473, 2204, 4226, 4255, 4497, 5112
BssNAI GTATAC 1 cut(s) 4342
BssNI GRCGYC 1 cut(s) 1961
BssSI CACGAG 1 cut(s) 4819
BssT1I CCWWGG 1 cut(s) 1473
Bst1107I GTATAC 1 cut(s) 4342
Bst2BI CACGAG 1 cut(s) 4819
Bst2UI CCWGG 4 cut(s) 403, 1235, 4227, 4256
Bst4CI ACNGT 9 cut(s) 537, 905, 1113, 1294, 1739, 2785, 3958, 4418, 5116
Bst6I CTCTTC 5 cut(s) 147, 2448, 4839, 5046, 5061
BstACI GRCGYC 1 cut(s) 1961
BstAFI CTTAAG 9 cut(s) 835, 1952, 2051, 2093, 2129, 3056, 4475, 4591, 4652
BstAPI GCANNNNNTGC 1 cut(s) 3863
BstAUI TGTACA 2 cut(s) 2012, 4535
BstBI TTCGAA 2 cut(s) 1266, 2585
BstC8I GCNNGC 5 cut(s) 454, 1229, 2696, 3852, 5331
BstDSI CCRYGG 3 cut(s) 2204, 4497, 5112
BstEII GGTNACC 1 cut(s) 4250
BstFNI CGCG 1 cut(s) 203
BstNI CCWGG 4 cut(s) 403, 1235, 4227, 4256
BstNSI RCATGY 3 cut(s) 1314, 1867, 3866
BstPI GGTNACC 1 cut(s) 4250
BstSCI CCNGG 5 cut(s) 401, 1018, 1233, 4225, 4254
BstSFI CTRYAG 6 cut(s) 1283, 3088, 3417, 4096, 4178, 4849
BstUI CGCG 1 cut(s) 203
BstV1I GCAGC 9 cut(s) 841, 2121, 2138, 2331, 2760, 2913, 4661, 5252, 5297
BstV2I GAAGAC 4 cut(s) 686, 2522, 2732, 5189
BstX2I RGATCY 5 cut(s) 87, 334, 3214, 3880, 4124
BstXI CCANNNNNNTGG 2 cut(s) 3190, 4566
BstYI RGATCY 5 cut(s) 87, 334, 3214, 3880, 4124
BstZ17I GTATAC 1 cut(s) 4342
BsuI GTATCC 2 cut(s) 1421, 1549
BsuRI GGCC 8 cut(s) 99, 990, 1361, 1472, 1979, 3990, 4086, 5135
BtgI CCRYGG 3 cut(s) 2204, 4497, 5112
BtgZI GCGATG 1 cut(s) 673
BtsIMutI CAGTG 6 cut(s) 1118, 1422, 3963, 4801, 4951, 5235
Cac8I GCNNGC 5 cut(s) 454, 1229, 2696, 3852, 5331
CaiI CAGNNNCTG 3 cut(s) 1234, 2115, 4848
Cfr10I RCCGGY 1 cut(s) 452
Cfr13I GGNCC 3 cut(s) 408, 988, 5133
CseI GACGC 3 cut(s) 192, 209, 1969
Csp6I GTAC 8 cut(s) 1096, 1295, 1591, 2013, 3224, 3606, 4438, 4536
CspCI CAANNNNNGTGG 2 cut(s) 1969, 2004
CviQI GTAC 8 cut(s) 1096, 1295, 1591, 2013, 3224, 3606, 4438, 4536
DraI TTTAAA 2 cut(s) 531, 3745
DraIII CACNNNGTG 3 cut(s) 1115, 3851, 3958
EaeI YGGCCR 1 cut(s) 97
Eam1104I CTCTTC 5 cut(s) 147, 2448, 4839, 5046, 5061
EarI CTCTTC 5 cut(s) 147, 2448, 4839, 5046, 5061
EciI GGCGGA 1 cut(s) 2210
Ecl136II GAGCTC 1 cut(s) 1409
Eco130I CCWWGG 1 cut(s) 1473
Eco147I AGGCCT 1 cut(s) 1361
Eco24I GRGCYC 2 cut(s) 1411, 4794
Eco31I GGTCTC 1 cut(s) 3149
Eco47I GGWCC 1 cut(s) 408
Eco53kI GAGCTC 1 cut(s) 1409
Eco57I CTGAAG 9 cut(s) 171, 435, 1054, 1931, 2576, 3775, 3790, 3795, 4454
Eco88I CYCGRG 2 cut(s) 176, 3823
Eco91I GGTNACC 1 cut(s) 4250
EcoICRI GAGCTC 1 cut(s) 1409
EcoO109I RGGNCCY 2 cut(s) 988, 5133
EcoO65I GGTNACC 1 cut(s) 4250
EcoRI GAATTC 1 cut(s) 5032
EcoRII CCWGG 4 cut(s) 401, 1233, 4225, 4254
EcoT14I CCWWGG 1 cut(s) 1473
EcoT22I ATGCAT 4 cut(s) 290, 1452, 3924, 5163
EcoT38I GRGCYC 2 cut(s) 1411, 4794
ErhI CCWWGG 1 cut(s) 1473
Esp3I CGTCTC 2 cut(s) 1952, 5237
FalI AAGNNNNNCTT 4 cut(s) 1052, 1084, 1986, 2018
FaqI GGGAC 7 cut(s) 182, 421, 1794, 2905, 3741, 4163, 4168
FauI CCCGC 2 cut(s) 485, 5270
FauNDI CATATG 3 cut(s) 2433, 2547, 4641
FbaI TGATCA 3 cut(s) 727, 910, 3349
FblI GTMKAC 1 cut(s) 4341
FriOI GRGCYC 2 cut(s) 1411, 4794
GsaI CCCAGC 2 cut(s) 472, 3763
GsuI CTGGAG 1 cut(s) 1376
HaeIII GGCC 8 cut(s) 99, 990, 1361, 1472, 1979, 3990, 4086, 5135
HapII CCGG 5 cut(s) 338, 453, 1019, 2969, 4697
HgaI GACGC 3 cut(s) 192, 209, 1969
Hin1I GRCGYC 1 cut(s) 1961
HincII GTYRAC 7 cut(s) 1443, 1512, 1542, 1855, 2330, 2912, 4275
HindII GTYRAC 7 cut(s) 1443, 1512, 1542, 1855, 2330, 2912, 4275
HindIII AAGCTT 4 cut(s) 209, 500, 1623, 1904
HpaI GTTAAC 1 cut(s) 1542
HpaII CCGG 5 cut(s) 338, 453, 1019, 2969, 4697
HphI GGTGA 4 cut(s) 722, 788, 2024, 3267
Hpy99I CGWCG 1 cut(s) 1491
HpyCH4III ACNGT 9 cut(s) 537, 905, 1113, 1294, 1739, 2785, 3958, 4418, 5116
HpyCH4IV ACGT 3 cut(s) 171, 2444, 4445
HpySE526I ACGT 3 cut(s) 171, 2444, 4445
Hsp92I GRCGYC 1 cut(s) 1961
Kpn2I TCCGGA 2 cut(s) 2968, 4696
KroI GCCGGC 1 cut(s) 452
KroNI GCCGGC 1 cut(s) 454
Ksp22I TGATCA 3 cut(s) 727, 910, 3349
KspAI GTTAAC 1 cut(s) 1542
LguI GCTCTTC 1 cut(s) 147
Lsp1109I GCAGC 9 cut(s) 841, 2121, 2138, 2331, 2760, 2913, 4661, 5252, 5297
LweI GCATC 7 cut(s) 910, 1855, 1887, 3925, 4564, 4854, 5148
MaeII ACGT 3 cut(s) 171, 2444, 4445
MbiI CCGCTC 1 cut(s) 1674
MfeI CAATTG 7 cut(s) 2658, 3045, 3292, 3813, 4078, 4773, 5280
MflI RGATCY 5 cut(s) 87, 334, 3214, 3880, 4124
MhlI GDGCHC 5 cut(s) 396, 1411, 4794, 4892, 5258
MlsI TGGCCA 1 cut(s) 99
MluI ACGCGT 1 cut(s) 201
MluNI TGGCCA 1 cut(s) 99
MlyI GAGTC 9 cut(s) 1945, 2080, 2317, 2509, 2536, 2746, 2899, 5051, 5285
MmeI TCCRAC 6 cut(s) 559, 631, 3355, 4652, 4926, 5135
Mox20I TGGCCA 1 cut(s) 99
Mph1103I ATGCAT 4 cut(s) 290, 1452, 3924, 5163
MroI TCCGGA 2 cut(s) 2968, 4696
MroNI GCCGGC 1 cut(s) 452
MroXI GAANNNNTTC 5 cut(s) 586, 798, 2457, 2964, 3522
MscI TGGCCA 1 cut(s) 99
MslI CAYNNNNRTG 9 cut(s) 2436, 2546, 2646, 3012, 3984, 4080, 4212, 4640, 5251
Msp20I TGGCCA 1 cut(s) 99
MspA1I CMGCKG 5 cut(s) 413, 2674, 2698, 3422, 5265
MspCI CTTAAG 9 cut(s) 835, 1952, 2051, 2093, 2129, 3056, 4475, 4591, 4652
MspI CCGG 5 cut(s) 338, 453, 1019, 2969, 4697
MspR9I CCNGG 5 cut(s) 403, 1020, 1235, 4227, 4256
MunI CAATTG 7 cut(s) 2658, 3045, 3292, 3813, 4078, 4773, 5280
Mva1269I GAATGC 4 cut(s) 33, 290, 792, 2541
MvaI CCWGG 4 cut(s) 403, 1235, 4227, 4256
MvnI CGCG 1 cut(s) 203
NaeI GCCGGC 1 cut(s) 454
NciI CCSGG 1 cut(s) 1020
NdeI CATATG 3 cut(s) 2433, 2547, 4641
NgoMIV GCCGGC 1 cut(s) 452
NlaIV GGNNCC 8 cut(s) 315, 409, 1165, 1900, 3580, 4232, 4408, 5134
NmeAIII GCCGAG 1 cut(s) 2922
NmuCI GTSAC 5 cut(s) 1414, 2272, 3273, 3843, 4460
NsiI ATGCAT 4 cut(s) 290, 1452, 3924, 5163
NspI RCATGY 3 cut(s) 1314, 1867, 3866
NspV TTCGAA 2 cut(s) 1266, 2585
OliI CACNNNNGTG 1 cut(s) 4080
PaeR7I CTCGAG 1 cut(s) 3823
PceI AGGCCT 1 cut(s) 1361
PciSI GCTCTTC 1 cut(s) 147
PcsI WCGNNNNNNNCGW 1 cut(s) 276
PctI GAATGC 4 cut(s) 33, 290, 792, 2541
PdiI GCCGGC 1 cut(s) 454
PdmI GAANNNNTTC 5 cut(s) 586, 798, 2457, 2964, 3522
PflFI GACNNNGTC 1 cut(s) 5193
PflMI CCANNNNNTGG 1 cut(s) 1234
PleI GAGTC 9 cut(s) 1944, 2079, 2316, 2508, 2535, 2745, 2898, 5050, 5284
PpsI GAGTC 9 cut(s) 1944, 2079, 2316, 2508, 2535, 2745, 2898, 5050, 5284
PshBI ATTAAT 3 cut(s) 462, 2420, 4016
PsiI TTATAA 2 cut(s) 747, 4310
Psp124BI GAGCTC 1 cut(s) 1411
Psp6I CCWGG 4 cut(s) 401, 1233, 4225, 4254
PspEI GGTNACC 1 cut(s) 4250
PspFI CCCAGC 2 cut(s) 468, 3759
PspGI CCWGG 4 cut(s) 401, 1233, 4225, 4254
PspN4I GGNNCC 8 cut(s) 315, 409, 1165, 1900, 3580, 4232, 4408, 5134
PspPI GGNCC 3 cut(s) 408, 988, 5133
PsrI GAACNNNNNNTAC 4 cut(s) 1377, 1409, 4430, 4462
PstI CTGCAG 1 cut(s) 3092
PstNI CAGNNNCTG 3 cut(s) 1234, 2115, 4848
PsuI RGATCY 5 cut(s) 87, 334, 3214, 3880, 4124
PsyI GACNNNGTC 1 cut(s) 5193
PvuII CAGCTG 2 cut(s) 2698, 3422
RsaI GTAC 8 cut(s) 1097, 1296, 1592, 2014, 3225, 3607, 4439, 4537
RsaNI GTAC 8 cut(s) 1096, 1295, 1591, 2013, 3224, 3606, 4438, 4536
RseI CAYNNNNRTG 9 cut(s) 2436, 2546, 2646, 3012, 3984, 4080, 4212, 4640, 5251
SacI GAGCTC 1 cut(s) 1411
SapI GCTCTTC 1 cut(s) 147
Sau96I GGNCC 3 cut(s) 408, 988, 5133
ScaI AGTACT 2 cut(s) 1296, 4439
SchI GAGTC 9 cut(s) 1945, 2080, 2317, 2509, 2536, 2746, 2899, 5051, 5285
ScrFI CCNGG 5 cut(s) 403, 1020, 1235, 4227, 4256
SduI GDGCHC 5 cut(s) 396, 1411, 4794, 4892, 5258
SfaNI GCATC 7 cut(s) 910, 1855, 1887, 3925, 4564, 4854, 5148
SfcI CTRYAG 6 cut(s) 1283, 3088, 3417, 4096, 4178, 4849
Sfr274I CTCGAG 1 cut(s) 3823
SfuI TTCGAA 2 cut(s) 1266, 2585
SinI GGWCC 1 cut(s) 408
SlaI CTCGAG 1 cut(s) 3823
SmiMI CAYNNNNRTG 9 cut(s) 2436, 2546, 2646, 3012, 3984, 4080, 4212, 4640, 5251
SseBI AGGCCT 1 cut(s) 1361
SstI GAGCTC 1 cut(s) 1411
StuI AGGCCT 1 cut(s) 1361
StyD4I CCNGG 5 cut(s) 401, 1018, 1233, 4225, 4254
StyI CCWWGG 1 cut(s) 1473
TaaI ACNGT 9 cut(s) 537, 905, 1113, 1294, 1739, 2785, 3958, 4418, 5116
TaiI ACGT 3 cut(s) 174, 2447, 4448
TatI WGTACW 5 cut(s) 1294, 2012, 3223, 4437, 4535
TauI GCSGC 3 cut(s) 1878, 2674, 3857
TscAI CASTG 6 cut(s) 1118, 1429, 3963, 4801, 4951, 5242
TseFI GTSAC 5 cut(s) 1414, 2272, 3273, 3843, 4460
Tsp45I GTSAC 5 cut(s) 1414, 2272, 3273, 3843, 4460
TspGWI ACGGA 5 cut(s) 436, 1178, 1475, 1568, 3959
TspRI CASTG 6 cut(s) 1118, 1429, 3963, 4801, 4951, 5242
Tth111I GACNNNGTC 1 cut(s) 5193
Van91I CCANNNNNTGG 1 cut(s) 1234
Vha464I CTTAAG 9 cut(s) 835, 1952, 2051, 2093, 2129, 3056, 4475, 4591, 4652
VpaK11BI GGWCC 1 cut(s) 408
VspI ATTAAT 3 cut(s) 462, 2420, 4016
XapI RAATTY 7 cut(s) 1001, 1350, 2168, 2973, 4068, 4392, 5032
XbaI TCTAGA 1 cut(s) 1300
XceI RCATGY 3 cut(s) 1314, 1867, 3866
XcmI CCANNNNNNNNNTGG 2 cut(s) 571, 850
XhoI CTCGAG 1 cut(s) 3823
XmiI GTMKAC 1 cut(s) 4341
XmnI GAANNNNTTC 5 cut(s) 586, 798, 2457, 2964, 3522
ZrmI AGTACT 2 cut(s) 1296, 4439
Zsp2I ATGCAT 4 cut(s) 290, 1452, 3924, 5163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.