FvH4_2g39320

dTDP-glucose 4,6-dehydratase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0011258
Physical Location & Seq
Forward (+)
28267757 .. 28270063
2307 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g39320.t1

Sequence Viewer

Length: 1020 bp
ATGGAGTTATGTCAAATTTCGGGTCAATTAGCATTCAAATCGGGTCACCTGCCCCGAAACTCTGTCACTCGAATGACCGGCGCACCGGACGTAAAATCTCCGAACCGGATATTTATCACCGGGATGGGTTTCGTCGGACAAGCGTTGGCGATTGACCTCAAGAAACAGGGATGGGATGTCTCTGGTACTTGTACAAGCATTAACAAGAAGAAGAGACTCGCGGAAGAAATGGGCTTTGATATGTACCTCTTTGATGCAAATGAACCAGATTTGGCCACCATTGATGTTATGAAGTTCCATACTCACATGGTTGTTTCAATTCCTCCGGTTACTGGTTTTGGTGATCCGATGCTGCAACATGAACAACTTTTGAGGAAAGCACTTTTGGGTGGAAATCTTCAGTGGCTTTGCTATTTGTCATCAACTAGTGTGTATGGAGATTGTGGAGGTGCATGGGTGGATGAGGATTATCCCCCTAACCCCACAAGGGAGGTGGGAAAATTAAGGTTAGCTGCTGAGAAAGGATGGTTACAGTTGGGGCAGAGTCTGGGCCTCTCGACAAAAGTATTTCGGCTTGGAGGTATCTATGGTCCTGGTAGAAGTGCTGTTGACACCATAATCAAGCAGGAAGCCTTATCAAAAAGTCAGAGAATGAGGGGATATAGGCAGTTCACATCAAGGATTCATGTTGAGGATATTTGTCAAGCCATTGAGGCCAGTATTCGAATGTCTTCCTCCAGGGAAATTTATAATATTGTTGATGATGACCCTTCCCCAAGAGAAGCAGTGTTCGAGTATGCAAGAGACTTGGTTGATAGAAAATGGCCAGGTTGGATCAAACAGTCTTCTGAAAAAAGAGAATCTACAGAGACTGATAAACAAGATGGTTTGTTGGGTGAGAAGAGAGTCTGCAATGCTCATATGAAGAAGGAGCTAGGAGTCAGGCTTCTTCATCCTAGTTATAGATCAGGATTACAAAATATTATTGACCAAATGGATAAACCATTTTCAACATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

340

Amino Acids

37.99

Weight (kDa)

8.42

Isoelectric Point (pI)

42.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 37 - 252 1.4e-09 NAD dependent epimerase/dehydratase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011258)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19690
fragaria_vesca FvH4_2g39320
malus_domestica MD08G1024600.v1.1
prunus_persica Prupe.1G374300_v2.0.a1
pyrus_communis pycom02g18430 pycom08g01950 pycom15g37000
rosa_chinensis RchiOBHm_Chr6g0301681
rosa_laevigata RLG00000011232
rosa_multiflora Rmu_sc0023062.1_g000003 Rmu_sc0034934.1_g000006 Rmu_sc0043116.1_g000002
rosa_roxburghii Rroxscaffold_7G00166530
rosa_rugosa Rorug06G0308000
rosa_samantha Rh6AG420600 Rh6BG427100 Rh6BG427200 Rh6CG433800 Rh6DG420500
rosa_wichuraiana Rw6G036390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 750
AarI CACCTGC 1 cut(s) 57
Acc36I ACCTGC 1 cut(s) 57
AccII CGCG 1 cut(s) 221
AciI CCGC 1 cut(s) 221
AclWI GGATC 2 cut(s) 338, 842
AcoI YGGCCR 2 cut(s) 273, 824
AcsI RAATTY 2 cut(s) 15, 744
AcuI CTGAAG 1 cut(s) 383
AfaI GTAC 3 cut(s) 187, 193, 245
AfiI CCNNNNNNNGG 2 cut(s) 332, 487
AgsI TTSAA 3 cut(s) 37, 318, 1011
AhlI ACTAGT 1 cut(s) 425
AjnI CCWGG 3 cut(s) 592, 737, 826
AjuI GAANNNNNNNTTGG 2 cut(s) 368, 400
AluBI AGCT 2 cut(s) 512, 934
AluI AGCT 2 cut(s) 512, 934
Alw26I GTCTC 4 cut(s) 184, 208, 798, 863
AlwI GGATC 2 cut(s) 338, 842
AlwNI CAGNNNCTG 2 cut(s) 547, 872
AoxI GGCC 4 cut(s) 273, 550, 714, 824
ApeKI GCWGC 2 cut(s) 352, 512
ApoI RAATTY 2 cut(s) 15, 744
ArsI GACNNNNNNTTYG 2 cut(s) 7, 39
Asp700I GAANNNNTTC 1 cut(s) 730
AspLEI GCGC 1 cut(s) 83
AspS9I GGNCC 2 cut(s) 550, 590
AsuC2I CCSGG 1 cut(s) 121
AsuHPI GGTGA 4 cut(s) 38, 109, 353, 908
AsuII TTCGAA 1 cut(s) 724
AvaII GGWCC 1 cut(s) 590
BalI TGGCCA 2 cut(s) 275, 826
BbsI GAAGAC 2 cut(s) 723, 837
BbvI GCAGC 2 cut(s) 339, 499
BccI CCATC 4 cut(s) 118, 165, 519, 878
BcgI CGANNNNNNTGC 2 cut(s) 21, 55
BciT130I CCWGG 3 cut(s) 594, 739, 828
BcnI CCSGG 1 cut(s) 121
BcoDI GTCTC 4 cut(s) 184, 208, 798, 863
BcuI ACTAGT 1 cut(s) 425
BfaI CTAG 3 cut(s) 426, 935, 957
BfmI CTRYAG 1 cut(s) 864
BfuAI ACCTGC 1 cut(s) 57
BglI GCCNNNNNGGC 1 cut(s) 713
BisI GCNGC 2 cut(s) 353, 513
BlsI GCNGC 2 cut(s) 354, 514
Bme1390I CCNGG 4 cut(s) 121, 594, 739, 828
Bme18I GGWCC 1 cut(s) 590
BmgT120I GGNCC 2 cut(s) 550, 590
BmrFI CCNGG 4 cut(s) 121, 594, 739, 828
BmsI GCATC 2 cut(s) 244, 339
BpiI GAAGAC 2 cut(s) 723, 837
BpmI CTGGAG 1 cut(s) 721
Bpu14I TTCGAA 1 cut(s) 724
BpuEI CTTGAG 1 cut(s) 143
BpuMI CCSGG 1 cut(s) 121
BsaBI GATNNNNATC 1 cut(s) 113
BsaJI CCNNGG 1 cut(s) 738
BsaWI WCCGGW 3 cut(s) 85, 105, 325
Bsc4I CCNNNNNNNGG 2 cut(s) 332, 487
Bse118I RCCGGY 1 cut(s) 77
Bse1I ACTGG 2 cut(s) 337, 717
Bse3DI GCAATG 1 cut(s) 919
Bse8I GATNNNNATC 1 cut(s) 113
BseBI CCWGG 3 cut(s) 594, 739, 828
BseDI CCNNGG 1 cut(s) 738
BseGI GGATG 6 cut(s) 129, 176, 181, 466, 530, 952
BseJI GATNNNNATC 1 cut(s) 113
BseLI CCNNNNNNNGG 2 cut(s) 332, 487
BseMI GCAATG 1 cut(s) 919
BseMII CTCAG 1 cut(s) 507
BseNI ACTGG 2 cut(s) 337, 717
BseXI GCAGC 2 cut(s) 339, 499
Bsh1236I CGCG 1 cut(s) 221
BshFI GGCC 4 cut(s) 275, 552, 716, 826
BsiSI CCGG 5 cut(s) 78, 86, 106, 120, 326
BslI CCNNNNNNNGG 2 cut(s) 332, 487
BsmAI GTCTC 4 cut(s) 184, 208, 798, 863
BsmI GAATGC 1 cut(s) 32
BsnI GGCC 4 cut(s) 275, 552, 716, 826
Bsp119I TTCGAA 1 cut(s) 724
Bsp1407I TGTACA 1 cut(s) 191
Bsp143I GATC 3 cut(s) 343, 834, 965
BspACI CCGC 1 cut(s) 221
BspANI GGCC 4 cut(s) 275, 552, 716, 826
BspCNI CTCAG 1 cut(s) 508
BspFNI CGCG 1 cut(s) 221
BspMI ACCTGC 1 cut(s) 57
BspPI GGATC 2 cut(s) 338, 842
BspT104I TTCGAA 1 cut(s) 724
BsrDI GCAATG 1 cut(s) 919
BsrFI RCCGGY 1 cut(s) 77
BsrGI TGTACA 1 cut(s) 191
BsrI ACTGG 2 cut(s) 337, 717
BssAI RCCGGY 1 cut(s) 77
BssECI CCNNGG 1 cut(s) 738
BssMI GATC 3 cut(s) 343, 834, 965
Bst2UI CCWGG 3 cut(s) 594, 739, 828
Bst4CI ACNGT 2 cut(s) 534, 843
Bst6I CTCTTC 2 cut(s) 206, 896
BstAUI TGTACA 1 cut(s) 191
BstBI TTCGAA 1 cut(s) 724
BstDEI CTNAG 1 cut(s) 516
BstEII GGTNACC 1 cut(s) 44
BstF5I GGATG 6 cut(s) 129, 176, 181, 466, 530, 952
BstFNI CGCG 1 cut(s) 221
BstHHI GCGC 1 cut(s) 83
BstKTI GATC 3 cut(s) 346, 837, 968
BstMAI GTCTC 4 cut(s) 184, 208, 798, 863
BstMBI GATC 3 cut(s) 343, 834, 965
BstMWI GCNNNNNNNGC 1 cut(s) 713
BstNI CCWGG 3 cut(s) 594, 739, 828
BstPI GGTNACC 1 cut(s) 44
BstSCI CCNGG 4 cut(s) 119, 592, 737, 826
BstSFI CTRYAG 1 cut(s) 864
BstUI CGCG 1 cut(s) 221
BstV1I GCAGC 2 cut(s) 339, 499
BstV2I GAAGAC 2 cut(s) 723, 837
BsuRI GGCC 4 cut(s) 275, 552, 716, 826
BtsCI GGATG 6 cut(s) 129, 176, 181, 466, 530, 952
BtsI GCAGTG 1 cut(s) 792
BtsIMutI CAGTG 2 cut(s) 407, 792
BveI ACCTGC 1 cut(s) 57
CaiI CAGNNNCTG 2 cut(s) 547, 872
CfoI GCGC 1 cut(s) 83
Cfr10I RCCGGY 1 cut(s) 77
Cfr13I GGNCC 2 cut(s) 550, 590
Csp6I GTAC 3 cut(s) 186, 192, 244
CspCI CAANNNNNGTGG 2 cut(s) 474, 509
CviAII CATG 4 cut(s) 307, 359, 453, 686
CviQI GTAC 3 cut(s) 186, 192, 244
DdeI CTNAG 1 cut(s) 516
DpnI GATC 3 cut(s) 345, 836, 967
DpnII GATC 3 cut(s) 343, 834, 965
EaeI YGGCCR 2 cut(s) 273, 824
Eam1104I CTCTTC 2 cut(s) 206, 896
EarI CTCTTC 2 cut(s) 206, 896
Eco47I GGWCC 1 cut(s) 590
Eco57I CTGAAG 1 cut(s) 383
Eco91I GGTNACC 1 cut(s) 44
EcoO65I GGTNACC 1 cut(s) 44
EcoRII CCWGG 3 cut(s) 592, 737, 826
FaeI CATG 4 cut(s) 310, 362, 456, 689
FatI CATG 4 cut(s) 306, 358, 452, 685
FauNDI CATATG 1 cut(s) 921
Fnu4HI GCNGC 2 cut(s) 353, 513
FokI GGATG 6 cut(s) 136, 183, 188, 473, 537, 939
Fsp4HI GCNGC 2 cut(s) 353, 513
FspBI CTAG 3 cut(s) 426, 935, 957
GlaI GCGC 1 cut(s) 82
GluI GCNGC 2 cut(s) 353, 513
GsuI CTGGAG 1 cut(s) 721
HaeIII GGCC 4 cut(s) 275, 552, 716, 826
HapII CCGG 5 cut(s) 78, 86, 106, 120, 326
HhaI GCGC 1 cut(s) 83
Hin1II CATG 4 cut(s) 310, 362, 456, 689
Hin6I GCGC 1 cut(s) 81
HinP1I GCGC 1 cut(s) 81
HincII GTYRAC 1 cut(s) 610
HindII GTYRAC 1 cut(s) 610
HinfI GANTC 6 cut(s) 216, 544, 682, 860, 906, 939
HpaII CCGG 5 cut(s) 78, 86, 106, 120, 326
HphI GGTGA 4 cut(s) 38, 109, 353, 908
Hpy166II GTNNAC 2 cut(s) 610, 672
Hpy188I TCNGA 5 cut(s) 102, 137, 348, 648, 850
Hpy188III TCNNGA 3 cut(s) 160, 556, 969
Hpy8I GTNNAC 2 cut(s) 610, 672
Hpy99I CGWCG 1 cut(s) 137
HpyAV CCTTC 2 cut(s) 780, 922
HpyCH4III ACNGT 2 cut(s) 534, 843
HpyCH4IV ACGT 1 cut(s) 90
HpyCH4V TGCA 5 cut(s) 257, 355, 452, 800, 912
HpyF10VI GCNNNNNNNGC 1 cut(s) 713
HpyF3I CTNAG 1 cut(s) 516
HpySE526I ACGT 1 cut(s) 90
Hsp92II CATG 4 cut(s) 310, 362, 456, 689
HspAI GCGC 1 cut(s) 81
Kzo9I GATC 3 cut(s) 343, 834, 965
LmnI GCTCC 1 cut(s) 931
Lsp1109I GCAGC 2 cut(s) 339, 499
LweI GCATC 2 cut(s) 244, 339
MaeI CTAG 3 cut(s) 426, 935, 957
MaeII ACGT 1 cut(s) 90
MaeIII GTNAC 4 cut(s) 44, 64, 328, 528
MalI GATC 3 cut(s) 345, 836, 967
MboI GATC 3 cut(s) 343, 834, 965
MboII GAAGA 9 cut(s) 220, 223, 236, 389, 723, 837, 913, 937, 941
MlsI TGGCCA 2 cut(s) 275, 826
MluCI AATT 5 cut(s) 15, 26, 318, 500, 744
MluNI TGGCCA 2 cut(s) 275, 826
MlyI GAGTC 4 cut(s) 210, 553, 915, 948
MmeI TCCRAC 2 cut(s) 115, 812
Mox20I TGGCCA 2 cut(s) 275, 826
MroXI GAANNNNTTC 1 cut(s) 730
MscI TGGCCA 2 cut(s) 275, 826
MseI TTAA 2 cut(s) 201, 503
MslI CAYNNNNRTG 2 cut(s) 71, 122
Msp20I TGGCCA 2 cut(s) 275, 826
MspI CCGG 5 cut(s) 78, 86, 106, 120, 326
MspR9I CCNGG 4 cut(s) 121, 594, 739, 828
Mva1269I GAATGC 1 cut(s) 32
MvaI CCWGG 3 cut(s) 594, 739, 828
MvnI CGCG 1 cut(s) 221
MwoI GCNNNNNNNGC 1 cut(s) 713
NciI CCSGG 1 cut(s) 121
NdeI CATATG 1 cut(s) 921
NdeII GATC 3 cut(s) 343, 834, 965
NlaIII CATG 4 cut(s) 310, 362, 456, 689
NmuCI GTSAC 2 cut(s) 44, 64
NspV TTCGAA 1 cut(s) 724
PaqCI CACCTGC 1 cut(s) 57
PctI GAATGC 1 cut(s) 32
PdmI GAANNNNTTC 1 cut(s) 730
PfeI GAWTC 2 cut(s) 682, 860
PkrI GCNGC 2 cut(s) 354, 514
PleI GAGTC 4 cut(s) 210, 552, 914, 947
PpsI GAGTC 4 cut(s) 210, 552, 914, 947
PsiI TTATAA 1 cut(s) 750
Psp6I CCWGG 3 cut(s) 592, 737, 826
PspEI GGTNACC 1 cut(s) 44
PspGI CCWGG 3 cut(s) 592, 737, 826
PspPI GGNCC 2 cut(s) 550, 590
PstNI CAGNNNCTG 2 cut(s) 547, 872
RsaI GTAC 3 cut(s) 187, 193, 245
RsaNI GTAC 3 cut(s) 186, 192, 244
RseI CAYNNNNRTG 2 cut(s) 71, 122
SaqAI TTAA 2 cut(s) 201, 503
SatI GCNGC 2 cut(s) 353, 513
Sau3AI GATC 3 cut(s) 343, 834, 965
Sau96I GGNCC 2 cut(s) 550, 590
SchI GAGTC 4 cut(s) 210, 553, 915, 948
ScrFI CCNGG 4 cut(s) 121, 594, 739, 828
SfaNI GCATC 2 cut(s) 244, 339
SfcI CTRYAG 1 cut(s) 864
SfuI TTCGAA 1 cut(s) 724
SinI GGWCC 1 cut(s) 590
SmiMI CAYNNNNRTG 2 cut(s) 71, 122
SmlI CTYRAG 1 cut(s) 158
SmoI CTYRAG 1 cut(s) 158
SpeI ACTAGT 1 cut(s) 425
Sse9I AATT 5 cut(s) 15, 26, 318, 500, 744
SsiI CCGC 1 cut(s) 221
SspI AATATT 2 cut(s) 754, 982
SspMI CTAG 3 cut(s) 426, 935, 957
StyD4I CCNGG 4 cut(s) 119, 592, 737, 826
TaaI ACNGT 2 cut(s) 534, 843
TaiI ACGT 1 cut(s) 93
TaqI TCGA 4 cut(s) 70, 557, 724, 792
TasI AATT 5 cut(s) 15, 26, 318, 500, 744
TatI WGTACW 1 cut(s) 191
TfiI GAWTC 2 cut(s) 682, 860
Tru1I TTAA 2 cut(s) 201, 503
Tru9I TTAA 2 cut(s) 201, 503
TscAI CASTG 2 cut(s) 407, 792
TseFI GTSAC 2 cut(s) 44, 64
TseI GCWGC 2 cut(s) 352, 512
Tsp45I GTSAC 2 cut(s) 44, 64
TspDTI ATGAA 6 cut(s) 276, 305, 375, 674, 938, 941
TspRI CASTG 2 cut(s) 407, 792
VpaK11BI GGWCC 1 cut(s) 590
XapI RAATTY 2 cut(s) 15, 744
XcmI CCANNNNNNNNNTGG 1 cut(s) 490
XmnI GAANNNNTTC 1 cut(s) 730
XspI CTAG 3 cut(s) 426, 935, 957
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.