pycom02g18430

dTDP-glucose 4,6-dehydratase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0011258
Physical Location & Seq
Forward (+)
16618611 .. 16620387
1777 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g18430.9

Sequence Viewer

Length: 1329 bp
ATGAAAGTCGATCTTCCTCCTGAAGATACGAAACGATCACTTGTGCGTTTGAAAAGATTAGTGCTATTGGGGACCAAAAGTGGCTATCACCCAGATGTTCTTCCGTCGAGTGCTACCACTTTAGCACCAGGTTGTTACTCAAGACATTTATGGAATCTCGACGAAAGTAAACTTCGTAGGAATTCTAATAGATGGTATACTAATAGATACTTATTAAGTAATACAGTTAGTAATTTTGTTAGTATTTGGTTAAAACAAATTCTATTTCTCTCTCTAAACTCTCTCTCTAATATCTCTGTGTTCATCCATCATTTCTTCTTGATTTTCCCTTCGATTCCCAAACATTGCAATGTAGTTAACATGGTATCAGAGTCACCAGGCTCATGCCATGGATTCTGGTGGTTTCTTTTCATAGTAGTTTACATTTTTTTTTCCTATTTCTGCGATTGTTGGGTTAATTGTTTGATTAGGAGATTTCTCCACCGACACCTTCACATCTCCCGAAACGTTCATCGCCGAAATGGGTTTCGTCGGCTAGCGCCTGGGGCGCGAGCTCAAGAACCACATATGAGATGTCTTTATTTACTTTTTGATGCAAATGAACTAGATGTGATCATCCTTGATGTTATCAAGAACCATACGCACATGGTTGTCTCCATTATTCTTGAAGTGTTAATGAGAGGACATAGGCATTTCACATCAAGAGTTTCTGTTGATGGTATTTGTCAAGCAATTATGGCCAGTATATGTACACCGTCATCCAGTTTTTTTGTATATAGTAGTCGAACCTTAATCATGGTGATTAAATCAATCACACTTACATGCAGAAATCACTTTTTGAATTCTTTGGTGGTGTTTGTTTGTTCAAGATTACAAATCTTGAGATTTCTTCAAGTTCTGATTTGTGTGCTCTCCAACTGTTTGAAGAAATGGGTCAGTGAATTATTTGATCTGTACTCTGTTCTCTGGTATGGAAGAAAGATTAGACATTTTCATTTGAAGGTTATGTTTTTTAGTCCATTTTCTCCGAATTCTAGAATAATTAGAATGAAACTTTTCTATTCTTTGTTCTCTTTGGGTTCTGCATCTTTGATGTTTTGCTTATCCTTGATAGTTCTAAAAGAGAGGTTGGGTTCATGCCTATGCTTGAATCAAAGGCTGCATGAGGATGAAGGCATTGCACATCCAGCTCGCCAGATTTGTGATCAGAGTATTGGGCACAAAGTTGTTCTAGTTCAAGAATGGCTTAGAGTTATACACAAAGTAGCAAACACACTTTCTTTTGGCTTGGAGCCATCTTTCTTTTGCCTTGAAGCAGTCTGCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

443

Amino Acids

51.36

Weight (kDa)

9.64

Isoelectric Point (pI)

49.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011258)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19690
fragaria_vesca FvH4_2g39320
malus_domestica MD08G1024600.v1.1
prunus_persica Prupe.1G374300_v2.0.a1
pyrus_communis pycom02g18430 pycom08g01950 pycom15g37000
rosa_chinensis RchiOBHm_Chr6g0301681
rosa_laevigata RLG00000011232
rosa_multiflora Rmu_sc0023062.1_g000003 Rmu_sc0034934.1_g000006 Rmu_sc0043116.1_g000002
rosa_roxburghii Rroxscaffold_7G00166530
rosa_rugosa Rorug06G0308000
rosa_samantha Rh6AG420600 Rh6BG427100 Rh6BG427200 Rh6CG433800 Rh6DG420500
rosa_wichuraiana Rw6G036390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 197
AccII CGCG 1 cut(s) 550
AclI AACGTT 1 cut(s) 507
AcoI YGGCCR 1 cut(s) 738
AcsI RAATTY 4 cut(s) 181, 258, 841, 1030
AcuI CTGAAG 1 cut(s) 42
AfaI GTAC 2 cut(s) 751, 956
AjnI CCWGG 3 cut(s) 127, 376, 541
AluBI AGCT 2 cut(s) 554, 1190
AluI AGCT 2 cut(s) 554, 1190
Alw21I GWGCWC 2 cut(s) 556, 912
Alw26I GTCTC 1 cut(s) 658
AoxI GGCC 1 cut(s) 738
ApeKI GCWGC 1 cut(s) 1159
ApoI RAATTY 4 cut(s) 181, 258, 841, 1030
Asp700I GAANNNNTTC 1 cut(s) 1055
AspLEI GCGC 2 cut(s) 541, 550
AspS9I GGNCC 1 cut(s) 72
AsuHPI GGTGA 3 cut(s) 80, 366, 811
AsuNHI GCTAGC 1 cut(s) 535
AvaII GGWCC 1 cut(s) 72
BaeGI GKGCMC 1 cut(s) 1221
BalI TGGCCA 1 cut(s) 740
BanII GRGCYC 1 cut(s) 556
Bbv12I GWGCWC 2 cut(s) 556, 912
BbvI GCAGC 1 cut(s) 1146
BccI CCATC 4 cut(s) 186, 315, 710, 1303
BciT130I CCWGG 3 cut(s) 129, 378, 543
BclI TGATCA 2 cut(s) 612, 1204
BcoDI GTCTC 1 cut(s) 658
BfaI CTAG 4 cut(s) 536, 605, 1035, 1232
BfoI RGCGCY 1 cut(s) 542
BisI GCNGC 1 cut(s) 1160
BlsI GCNGC 1 cut(s) 1161
Bme1390I CCNGG 3 cut(s) 129, 378, 543
Bme18I GGWCC 1 cut(s) 72
BmgT120I GGNCC 1 cut(s) 72
BmiI GGNNCC 2 cut(s) 73, 1293
BmrFI CCNGG 3 cut(s) 129, 378, 543
BmsI GCATC 2 cut(s) 583, 1094
BmtI GCTAGC 1 cut(s) 539
BpuEI CTTGAG 3 cut(s) 124, 540, 901
BsaJI CCNNGG 2 cut(s) 388, 542
Bse1I ACTGG 2 cut(s) 741, 762
Bse3DI GCAATG 3 cut(s) 343, 355, 1176
BseBI CCWGG 3 cut(s) 129, 378, 543
BseDI CCNNGG 2 cut(s) 388, 542
BseGI GGATG 5 cut(s) 303, 615, 758, 1174, 1183
BseMI GCAATG 3 cut(s) 343, 355, 1176
BseNI ACTGG 2 cut(s) 741, 762
BseSI GKGCMC 1 cut(s) 1221
BseXI GCAGC 1 cut(s) 1146
Bsh1236I CGCG 1 cut(s) 550
BshFI GGCC 1 cut(s) 740
BsiHKAI GWGCWC 2 cut(s) 556, 912
BslFI GGGAC 1 cut(s) 85
BsmAI GTCTC 1 cut(s) 658
BsmFI GGGAC 1 cut(s) 85
BsnI GGCC 1 cut(s) 740
Bsp1286I GDGCHC 3 cut(s) 556, 912, 1221
Bsp1407I TGTACA 1 cut(s) 749
Bsp143I GATC 5 cut(s) 10, 35, 612, 949, 1204
Bsp19I CCATGG 1 cut(s) 388
BspANI GGCC 1 cut(s) 740
BspFNI CGCG 1 cut(s) 550
BspLI GGNNCC 2 cut(s) 73, 1293
BspOI GCTAGC 1 cut(s) 539
BsrDI GCAATG 3 cut(s) 343, 355, 1176
BsrGI TGTACA 1 cut(s) 749
BsrI ACTGG 2 cut(s) 741, 762
BssECI CCNNGG 2 cut(s) 388, 542
BssMI GATC 5 cut(s) 10, 35, 612, 949, 1204
BssNAI GTATAC 1 cut(s) 198
BssT1I CCWWGG 1 cut(s) 388
Bst1107I GTATAC 1 cut(s) 198
Bst2UI CCWGG 3 cut(s) 129, 378, 543
Bst4CI ACNGT 3 cut(s) 226, 756, 920
BstAUI TGTACA 1 cut(s) 749
BstC8I GCNNGC 3 cut(s) 537, 552, 1192
BstDEI CTNAG 1 cut(s) 1247
BstDSI CCRYGG 1 cut(s) 388
BstF5I GGATG 5 cut(s) 303, 615, 758, 1174, 1183
BstFNI CGCG 1 cut(s) 550
BstH2I RGCGCY 1 cut(s) 542
BstHHI GCGC 2 cut(s) 541, 550
BstKTI GATC 5 cut(s) 13, 38, 615, 952, 1207
BstMAI GTCTC 1 cut(s) 658
BstMBI GATC 5 cut(s) 10, 35, 612, 949, 1204
BstMWI GCNNNNNNNGC 4 cut(s) 545, 547, 737, 1187
BstNI CCWGG 3 cut(s) 129, 378, 543
BstNSI RCATGY 1 cut(s) 825
BstSCI CCNGG 3 cut(s) 127, 376, 541
BstSLI GKGCMC 1 cut(s) 1221
BstUI CGCG 1 cut(s) 550
BstV1I GCAGC 1 cut(s) 1146
BstZ17I GTATAC 1 cut(s) 198
BsuRI GGCC 1 cut(s) 740
BtgI CCRYGG 1 cut(s) 388
BtgZI GCGATG 1 cut(s) 497
BtsCI GGATG 5 cut(s) 303, 615, 758, 1174, 1183
BtsIMutI CAGTG 1 cut(s) 943
Cac8I GCNNGC 3 cut(s) 537, 552, 1192
CfoI GCGC 2 cut(s) 541, 550
Cfr13I GGNCC 1 cut(s) 72
CsiI ACCWGGT 1 cut(s) 127
Csp6I GTAC 2 cut(s) 750, 955
CviAII CATG 8 cut(s) 361, 384, 389, 646, 796, 822, 1137, 1163
CviQI GTAC 2 cut(s) 750, 955
DdeI CTNAG 1 cut(s) 1247
DpnI GATC 5 cut(s) 12, 37, 614, 951, 1206
DpnII GATC 5 cut(s) 10, 35, 612, 949, 1204
EaeI YGGCCR 1 cut(s) 738
Ecl136II GAGCTC 1 cut(s) 554
Eco130I CCWWGG 1 cut(s) 388
Eco24I GRGCYC 1 cut(s) 556
Eco47I GGWCC 1 cut(s) 72
Eco53kI GAGCTC 1 cut(s) 554
Eco57I CTGAAG 1 cut(s) 42
EcoICRI GAGCTC 1 cut(s) 554
EcoRI GAATTC 3 cut(s) 181, 841, 1030
EcoRII CCWGG 3 cut(s) 127, 376, 541
EcoT14I CCWWGG 1 cut(s) 388
EcoT38I GRGCYC 1 cut(s) 556
ErhI CCWWGG 1 cut(s) 388
FaeI CATG 8 cut(s) 364, 387, 392, 649, 799, 825, 1140, 1166
FalI AAGNNNNNCTT 3 cut(s) 29, 1230, 1262
FaqI GGGAC 1 cut(s) 85
FatI CATG 8 cut(s) 360, 383, 388, 645, 795, 821, 1136, 1162
FauNDI CATATG 1 cut(s) 567
FbaI TGATCA 2 cut(s) 612, 1204
FblI GTMKAC 1 cut(s) 197
Fnu4HI GCNGC 1 cut(s) 1160
FokI GGATG 5 cut(s) 290, 602, 745, 1170, 1181
FriOI GRGCYC 1 cut(s) 556
Fsp4HI GCNGC 1 cut(s) 1160
FspBI CTAG 4 cut(s) 536, 605, 1035, 1232
GlaI GCGC 2 cut(s) 540, 549
GluI GCNGC 1 cut(s) 1160
HaeII RGCGCY 1 cut(s) 542
HaeIII GGCC 1 cut(s) 740
HhaI GCGC 2 cut(s) 541, 550
Hin1II CATG 8 cut(s) 364, 387, 392, 649, 799, 825, 1140, 1166
Hin6I GCGC 2 cut(s) 539, 548
HinP1I GCGC 2 cut(s) 539, 548
HincII GTYRAC 1 cut(s) 358
HindII GTYRAC 1 cut(s) 358
HinfI GANTC 5 cut(s) 154, 334, 371, 393, 1150
HpaI GTTAAC 1 cut(s) 358
HphI GGTGA 3 cut(s) 80, 366, 811
Hpy166II GTNNAC 5 cut(s) 170, 198, 358, 421, 752
Hpy188I TCNGA 4 cut(s) 370, 900, 1029, 1209
Hpy8I GTNNAC 5 cut(s) 170, 198, 358, 421, 752
Hpy99I CGWCG 3 cut(s) 109, 164, 534
HpyAV CCTTC 4 cut(s) 339, 500, 994, 1166
HpyCH4III ACNGT 3 cut(s) 226, 756, 920
HpyCH4IV ACGT 1 cut(s) 507
HpyCH4V TGCA 7 cut(s) 348, 596, 825, 1085, 1162, 1181, 1323
HpyF10VI GCNNNNNNNGC 4 cut(s) 545, 547, 737, 1187
HpyF3I CTNAG 1 cut(s) 1247
HpySE526I ACGT 1 cut(s) 507
Hsp92II CATG 8 cut(s) 364, 387, 392, 649, 799, 825, 1140, 1166
HspAI GCGC 2 cut(s) 539, 548
Ksp22I TGATCA 2 cut(s) 612, 1204
KspAI GTTAAC 1 cut(s) 358
Kzo9I GATC 5 cut(s) 10, 35, 612, 949, 1204
LmnI GCTCC 1 cut(s) 1291
Lsp1109I GCAGC 1 cut(s) 1146
LweI GCATC 2 cut(s) 583, 1094
MabI ACCWGGT 1 cut(s) 127
MaeI CTAG 4 cut(s) 536, 605, 1035, 1232
MaeII ACGT 1 cut(s) 507
MaeIII GTNAC 2 cut(s) 134, 372
MalI GATC 5 cut(s) 12, 37, 614, 951, 1206
MboI GATC 5 cut(s) 10, 35, 612, 949, 1204
MboII GAAGA 7 cut(s) 5, 35, 92, 307, 881, 937, 987
MhlI GDGCHC 3 cut(s) 556, 912, 1221
MlsI TGGCCA 1 cut(s) 740
MluCI AATT 9 cut(s) 181, 232, 258, 457, 732, 841, 941, 1030, 1041
MluNI TGGCCA 1 cut(s) 740
MlyI GAGTC 1 cut(s) 380
MmeI TCCRAC 1 cut(s) 939
MnlI CCTC 4 cut(s) 27, 674, 1119, 1159
Mox20I TGGCCA 1 cut(s) 740
MroXI GAANNNNTTC 1 cut(s) 1055
MscI TGGCCA 1 cut(s) 740
MseI TTAA 7 cut(s) 215, 251, 357, 456, 674, 791, 804
MslI CAYNNNNRTG 5 cut(s) 93, 348, 820, 1141, 1167
Msp20I TGGCCA 1 cut(s) 740
MspR9I CCNGG 3 cut(s) 129, 378, 543
MvaI CCWGG 3 cut(s) 129, 378, 543
MvnI CGCG 1 cut(s) 550
MwoI GCNNNNNNNGC 4 cut(s) 545, 547, 737, 1187
NcoI CCATGG 1 cut(s) 388
NdeI CATATG 1 cut(s) 567
NdeII GATC 5 cut(s) 10, 35, 612, 949, 1204
NheI GCTAGC 1 cut(s) 535
NlaIII CATG 8 cut(s) 364, 387, 392, 649, 799, 825, 1140, 1166
NlaIV GGNNCC 2 cut(s) 73, 1293
NmuCI GTSAC 1 cut(s) 372
NspI RCATGY 1 cut(s) 825
PdmI GAANNNNTTC 1 cut(s) 1055
PfeI GAWTC 4 cut(s) 154, 334, 393, 1150
PkrI GCNGC 1 cut(s) 1161
PleI GAGTC 1 cut(s) 379
PpsI GAGTC 1 cut(s) 379
Psp124BI GAGCTC 1 cut(s) 556
Psp1406I AACGTT 1 cut(s) 507
Psp6I CCWGG 3 cut(s) 127, 376, 541
PspGI CCWGG 3 cut(s) 127, 376, 541
PspN4I GGNNCC 2 cut(s) 73, 1293
PspPI GGNCC 1 cut(s) 72
RsaI GTAC 2 cut(s) 751, 956
RsaNI GTAC 2 cut(s) 750, 955
RseI CAYNNNNRTG 5 cut(s) 93, 348, 820, 1141, 1167
SacI GAGCTC 1 cut(s) 556
SaqAI TTAA 7 cut(s) 215, 251, 357, 456, 674, 791, 804
SatI GCNGC 1 cut(s) 1160
Sau3AI GATC 5 cut(s) 10, 35, 612, 949, 1204
Sau96I GGNCC 1 cut(s) 72
SchI GAGTC 1 cut(s) 380
ScrFI CCNGG 3 cut(s) 129, 378, 543
SduI GDGCHC 3 cut(s) 556, 912, 1221
SetI ASST 8 cut(s) 133, 492, 510, 556, 791, 1005, 1130, 1192
SexAI ACCWGGT 1 cut(s) 127
SfaNI GCATC 2 cut(s) 583, 1094
SinI GGWCC 1 cut(s) 72
SmiMI CAYNNNNRTG 5 cut(s) 93, 348, 820, 1141, 1167
SmlI CTYRAG 3 cut(s) 139, 555, 880
SmoI CTYRAG 3 cut(s) 139, 555, 880
Sse9I AATT 9 cut(s) 181, 232, 258, 457, 732, 841, 941, 1030, 1041
SspMI CTAG 4 cut(s) 536, 605, 1035, 1232
SstI GAGCTC 1 cut(s) 556
StyD4I CCNGG 3 cut(s) 127, 376, 541
StyI CCWWGG 1 cut(s) 388
TaaI ACNGT 3 cut(s) 226, 756, 920
TaiI ACGT 1 cut(s) 510
TaqI TCGA 5 cut(s) 9, 107, 159, 332, 784
TasI AATT 9 cut(s) 181, 232, 258, 457, 732, 841, 941, 1030, 1041
TatI WGTACW 2 cut(s) 749, 954
TfiI GAWTC 4 cut(s) 154, 334, 393, 1150
Tru1I TTAA 7 cut(s) 215, 251, 357, 456, 674, 791, 804
Tru9I TTAA 7 cut(s) 215, 251, 357, 456, 674, 791, 804
TscAI CASTG 1 cut(s) 943
TseFI GTSAC 1 cut(s) 372
TseI GCWGC 1 cut(s) 1159
Tsp45I GTSAC 1 cut(s) 372
TspDTI ATGAA 9 cut(s) 17, 292, 400, 500, 615, 983, 1064, 1125, 1185
TspGWI ACGGA 1 cut(s) 93
TspRI CASTG 1 cut(s) 943
VpaK11BI GGWCC 1 cut(s) 72
XapI RAATTY 4 cut(s) 181, 258, 841, 1030
XbaI TCTAGA 1 cut(s) 1034
XceI RCATGY 1 cut(s) 825
XmiI GTMKAC 1 cut(s) 197
XmnI GAANNNNTTC 1 cut(s) 1055
XspI CTAG 4 cut(s) 536, 605, 1035, 1232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.