RchiOBHm_Chr6g0301681

dTDP-glucose 4,6-dehydratase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0011258
Physical Location & Seq
Reverse (-)
62018033 .. 62020299
2267 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27096

Sequence Viewer

Length: 1020 bp
ATGGAGTTGTGTCAAATTTCGGGTCAATTGGCATTCAAATCGGGTCATCTGCCCCCAAGCTCCGTCATTCGAATGACCTGTGCACCGGACGTAAAATCTCGGAACAGGATGTTCATCGCCGGAATTGGTTTCGTCGGACAGGCGTTGACGCTTGACCTCAAGAAGCAGGGATGGGATGTCTCTGGGACTTGTACAAGCATTACCAAGAAGAAGAAACTCGAGGAAGAAATGGGCTTTGATATGTACCTATTTGATGCAAATGAACCAGATGTGACCACCATGGATGTTATGAAGCTCCATACTCACATGGTTGTGTCAATCCCTCCAATTCCTGGTATTGGTGATCCGATGCTGCAACATGAACAACTTCTGAGGAAAGCACTTTTGGGGGGAAATCTTCAATGGCTTTGCTATTTGTCCTCAACTAGTGTATATGGAAATTGTGGTGGTGAATGGGTAGATGAGGATTATCCCCCTAACCCCACAAGGGAAGTGGGAAAATTAAGGTTAGCTGCTGAGAAAGGATGGTTACACTTGGGGCAGAGTTTGGGTATCTCAACAAAAGTATTTCGGCTCGGAGGTATCTATGGTCCTGGTAGAAGTGCTGTTGACACCATAATCAAGCAGGAAGCCTTATCAAAAAGTCAGAGAATGAGAGGATATAGGCAGTTCACATCAAGGATTCATGTTGAGGACATTTGTCAAGCCATTGAGGCCAGTATTTGCACAGCTTCCTCCAGGGAAATTTATAATATTGTTGATGATGACCCTGCCCCAAGGGAAGCAGTGTTTGAGTATGCAAGAGACTTGGTTGACAAAAAATGGCCAGGCTGGATCAAACAGGCTTCTGAACAAAGAGAATCTGCAGTCACTGTTATGAAAGATGGTTCGATGGGTGAGAAGAGAGTTTGCAATGCTCGTATGAAGAAGGAACTAGGACTTGGGCTTCTTCATCCTAGTTATAGATCAGGATTACAAAGTATTATTGACCAAATGGATAAACCATTTTCAACATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

339

Amino Acids

37.82

Weight (kDa)

8.04

Isoelectric Point (pI)

35.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 38 - 252 6e-08 NAD dependent epimerase/dehydratase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011258)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19690
fragaria_vesca FvH4_2g39320
malus_domestica MD08G1024600.v1.1
prunus_persica Prupe.1G374300_v2.0.a1
pyrus_communis pycom02g18430 pycom08g01950 pycom15g37000
rosa_chinensis RchiOBHm_Chr6g0301681
rosa_laevigata RLG00000011232
rosa_multiflora Rmu_sc0023062.1_g000003 Rmu_sc0034934.1_g000006 Rmu_sc0043116.1_g000002
rosa_roxburghii Rroxscaffold_7G00166530
rosa_rugosa Rorug06G0308000
rosa_samantha Rh6AG420600 Rh6BG427100 Rh6BG427200 Rh6CG433800 Rh6DG420500
rosa_wichuraiana Rw6G036390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 750
AccB7I CCANNNNNTGG 1 cut(s) 332
AclWI GGATC 2 cut(s) 338, 842
AcoI YGGCCR 1 cut(s) 824
AcsI RAATTY 2 cut(s) 15, 744
AfaI GTAC 2 cut(s) 193, 245
AfiI CCNNNNNNNGG 3 cut(s) 332, 338, 487
AgsI TTSAA 3 cut(s) 37, 401, 1011
AhlI ACTAGT 1 cut(s) 425
AjnI CCWGG 4 cut(s) 331, 592, 737, 826
AjuI GAANNNNNNNTTGG 4 cut(s) 368, 400, 924, 956
AluBI AGCT 4 cut(s) 60, 295, 512, 731
AluI AGCT 4 cut(s) 60, 295, 512, 731
Alw21I GWGCWC 1 cut(s) 85
Alw26I GTCTC 2 cut(s) 184, 798
Alw44I GTGCAC 1 cut(s) 81
AlwI GGATC 2 cut(s) 338, 842
AlwNI CAGNNNCTG 1 cut(s) 872
Ama87I CYCGRG 1 cut(s) 218
AoxI GGCC 2 cut(s) 714, 824
ApaLI GTGCAC 1 cut(s) 81
ApeKI GCWGC 2 cut(s) 352, 512
ApoI RAATTY 2 cut(s) 15, 744
ArsI GACNNNNNNTTYG 2 cut(s) 7, 39
Asp700I GAANNNNTTC 1 cut(s) 366
AspS9I GGNCC 1 cut(s) 590
AsuHPI GGTGA 3 cut(s) 353, 461, 908
AsuII TTCGAA 1 cut(s) 70
AvaI CYCGRG 1 cut(s) 218
AvaII GGWCC 1 cut(s) 590
BaeGI GKGCMC 1 cut(s) 85
BalI TGGCCA 1 cut(s) 826
Bbv12I GWGCWC 1 cut(s) 85
BbvI GCAGC 2 cut(s) 339, 499
BccI CCATC 4 cut(s) 165, 519, 878, 886
BcgI CGANNNNNNTGC 2 cut(s) 21, 55
BciT130I CCWGG 4 cut(s) 333, 594, 739, 828
BcoDI GTCTC 2 cut(s) 184, 798
BcuI ACTAGT 1 cut(s) 425
BfaI CTAG 3 cut(s) 426, 935, 957
BfmI CTRYAG 1 cut(s) 864
BglI GCCNNNNNGGC 1 cut(s) 713
BisI GCNGC 2 cut(s) 353, 513
BlsI GCNGC 2 cut(s) 354, 514
Bme1390I CCNGG 4 cut(s) 333, 594, 739, 828
Bme18I GGWCC 1 cut(s) 590
BmeT110I CYCGRG 1 cut(s) 218
BmgT120I GGNCC 1 cut(s) 590
BmrFI CCNGG 4 cut(s) 333, 594, 739, 828
BmsI GCATC 2 cut(s) 244, 339
BoxI GACNNNNGTC 1 cut(s) 699
BpmI CTGGAG 1 cut(s) 721
Bpu14I TTCGAA 1 cut(s) 70
BpuEI CTTGAG 1 cut(s) 143
BsaBI GATNNNNATC 1 cut(s) 113
BsaJI CCNNGG 3 cut(s) 279, 738, 776
BsaWI WCCGGW 1 cut(s) 85
Bsc4I CCNNNNNNNGG 3 cut(s) 332, 338, 487
Bse1I ACTGG 1 cut(s) 717
Bse3DI GCAATG 1 cut(s) 919
Bse8I GATNNNNATC 1 cut(s) 113
BseBI CCWGG 4 cut(s) 333, 594, 739, 828
BseDI CCNNGG 3 cut(s) 279, 738, 776
BseGI GGATG 6 cut(s) 114, 176, 181, 289, 530, 952
BseJI GATNNNNATC 1 cut(s) 113
BseLI CCNNNNNNNGG 3 cut(s) 332, 338, 487
BseMI GCAATG 1 cut(s) 919
BseMII CTCAG 2 cut(s) 362, 507
BseNI ACTGG 1 cut(s) 717
BseSI GKGCMC 1 cut(s) 85
BseXI GCAGC 2 cut(s) 339, 499
BshFI GGCC 2 cut(s) 716, 826
BsiHKAI GWGCWC 1 cut(s) 85
BsiHKCI CYCGRG 1 cut(s) 218
BsiSI CCGG 2 cut(s) 86, 120
BslFI GGGAC 1 cut(s) 199
BslI CCNNNNNNNGG 3 cut(s) 332, 338, 487
BsmAI GTCTC 2 cut(s) 184, 798
BsmFI GGGAC 1 cut(s) 199
BsmI GAATGC 1 cut(s) 32
BsnI GGCC 2 cut(s) 716, 826
BsoBI CYCGRG 1 cut(s) 218
Bsp119I TTCGAA 1 cut(s) 70
Bsp1286I GDGCHC 1 cut(s) 85
Bsp1407I TGTACA 1 cut(s) 191
Bsp143I GATC 3 cut(s) 343, 834, 965
Bsp19I CCATGG 1 cut(s) 279
BspANI GGCC 2 cut(s) 716, 826
BspCNI CTCAG 2 cut(s) 363, 508
BspMAI CTGCAG 1 cut(s) 868
BspPI GGATC 2 cut(s) 338, 842
BspT104I TTCGAA 1 cut(s) 70
BsrDI GCAATG 1 cut(s) 919
BsrGI TGTACA 1 cut(s) 191
BsrI ACTGG 1 cut(s) 717
BssECI CCNNGG 3 cut(s) 279, 738, 776
BssMI GATC 3 cut(s) 343, 834, 965
BssT1I CCWWGG 2 cut(s) 279, 776
Bst2UI CCWGG 4 cut(s) 333, 594, 739, 828
Bst4CI ACNGT 1 cut(s) 874
Bst6I CTCTTC 1 cut(s) 896
BstAUI TGTACA 1 cut(s) 191
BstBI TTCGAA 1 cut(s) 70
BstDEI CTNAG 2 cut(s) 371, 516
BstDSI CCRYGG 1 cut(s) 279
BstF5I GGATG 6 cut(s) 114, 176, 181, 289, 530, 952
BstKTI GATC 3 cut(s) 346, 837, 968
BstMAI GTCTC 2 cut(s) 184, 798
BstMBI GATC 3 cut(s) 343, 834, 965
BstMWI GCNNNNNNNGC 1 cut(s) 713
BstNI CCWGG 4 cut(s) 333, 594, 739, 828
BstPAI GACNNNNGTC 1 cut(s) 699
BstSCI CCNGG 4 cut(s) 331, 592, 737, 826
BstSFI CTRYAG 1 cut(s) 864
BstSLI GKGCMC 1 cut(s) 85
BstV1I GCAGC 2 cut(s) 339, 499
BsuRI GGCC 2 cut(s) 716, 826
BtgI CCRYGG 1 cut(s) 279
BtgZI GCGATG 1 cut(s) 100
BtsCI GGATG 6 cut(s) 114, 176, 181, 289, 530, 952
BtsI GCAGTG 1 cut(s) 792
BtsIMutI CAGTG 2 cut(s) 792, 870
CaiI CAGNNNCTG 1 cut(s) 872
Cfr13I GGNCC 1 cut(s) 590
CseI GACGC 1 cut(s) 157
Csp6I GTAC 2 cut(s) 192, 244
CspCI CAANNNNNGTGG 2 cut(s) 474, 509
CviAII CATG 5 cut(s) 280, 307, 359, 686, 1014
CviQI GTAC 2 cut(s) 192, 244
DdeI CTNAG 2 cut(s) 371, 516
DpnI GATC 3 cut(s) 345, 836, 967
DpnII GATC 3 cut(s) 343, 834, 965
EaeI YGGCCR 1 cut(s) 824
Eam1104I CTCTTC 1 cut(s) 896
EarI CTCTTC 1 cut(s) 896
Eco130I CCWWGG 2 cut(s) 279, 776
Eco47I GGWCC 1 cut(s) 590
Eco88I CYCGRG 1 cut(s) 218
EcoRII CCWGG 4 cut(s) 331, 592, 737, 826
EcoT14I CCWWGG 2 cut(s) 279, 776
ErhI CCWWGG 2 cut(s) 279, 776
FaeI CATG 5 cut(s) 283, 310, 362, 689, 1017
FaqI GGGAC 1 cut(s) 199
FatI CATG 5 cut(s) 279, 306, 358, 685, 1013
Fnu4HI GCNGC 2 cut(s) 353, 513
FokI GGATG 6 cut(s) 121, 183, 188, 296, 537, 939
Fsp4HI GCNGC 2 cut(s) 353, 513
FspBI CTAG 3 cut(s) 426, 935, 957
GluI GCNGC 2 cut(s) 353, 513
GsuI CTGGAG 1 cut(s) 721
HaeIII GGCC 2 cut(s) 716, 826
HapII CCGG 2 cut(s) 86, 120
HgaI GACGC 1 cut(s) 157
Hin1II CATG 5 cut(s) 283, 310, 362, 689, 1017
HincII GTYRAC 3 cut(s) 147, 610, 814
HindII GTYRAC 3 cut(s) 147, 610, 814
HinfI GANTC 2 cut(s) 682, 860
HpaII CCGG 2 cut(s) 86, 120
HphI GGTGA 3 cut(s) 353, 461, 908
Hpy166II GTNNAC 5 cut(s) 83, 147, 610, 672, 814
Hpy188I TCNGA 7 cut(s) 102, 137, 348, 372, 578, 648, 850
Hpy188III TCNNGA 2 cut(s) 160, 969
Hpy8I GTNNAC 5 cut(s) 83, 147, 610, 672, 814
Hpy99I CGWCG 1 cut(s) 137
HpyAV CCTTC 1 cut(s) 922
HpyCH4III ACNGT 1 cut(s) 874
HpyCH4IV ACGT 1 cut(s) 90
HpyCH4V TGCA 7 cut(s) 83, 257, 355, 726, 800, 866, 912
HpyF10VI GCNNNNNNNGC 1 cut(s) 713
HpyF3I CTNAG 2 cut(s) 371, 516
HpySE526I ACGT 1 cut(s) 90
Hsp92II CATG 5 cut(s) 283, 310, 362, 689, 1017
Kzo9I GATC 3 cut(s) 343, 834, 965
LmnI GCTCC 2 cut(s) 65, 300
Lsp1109I GCAGC 2 cut(s) 339, 499
LweI GCATC 2 cut(s) 244, 339
MaeI CTAG 3 cut(s) 426, 935, 957
MaeII ACGT 1 cut(s) 90
MaeIII GTNAC 3 cut(s) 271, 528, 868
MalI GATC 3 cut(s) 345, 836, 967
MboI GATC 3 cut(s) 343, 834, 965
MboII GAAGA 7 cut(s) 220, 223, 236, 389, 913, 937, 941
MfeI CAATTG 1 cut(s) 26
MhlI GDGCHC 1 cut(s) 85
MlsI TGGCCA 1 cut(s) 826
MluCI AATT 7 cut(s) 15, 26, 123, 327, 439, 500, 744
MluNI TGGCCA 1 cut(s) 826
MmeI TCCRAC 1 cut(s) 115
Mox20I TGGCCA 1 cut(s) 826
MroXI GAANNNNTTC 1 cut(s) 366
MscI TGGCCA 1 cut(s) 826
MseI TTAA 1 cut(s) 503
MslI CAYNNNNRTG 3 cut(s) 71, 311, 875
Msp20I TGGCCA 1 cut(s) 826
MspI CCGG 2 cut(s) 86, 120
MspR9I CCNGG 4 cut(s) 333, 594, 739, 828
MunI CAATTG 1 cut(s) 26
Mva1269I GAATGC 1 cut(s) 32
MvaI CCWGG 4 cut(s) 333, 594, 739, 828
MwoI GCNNNNNNNGC 1 cut(s) 713
NcoI CCATGG 1 cut(s) 279
NdeII GATC 3 cut(s) 343, 834, 965
NlaIII CATG 5 cut(s) 283, 310, 362, 689, 1017
NmuCI GTSAC 2 cut(s) 271, 868
NspV TTCGAA 1 cut(s) 70
PaeR7I CTCGAG 1 cut(s) 218
PctI GAATGC 1 cut(s) 32
PdmI GAANNNNTTC 1 cut(s) 366
PfeI GAWTC 2 cut(s) 682, 860
PflMI CCANNNNNTGG 1 cut(s) 332
PkrI GCNGC 2 cut(s) 354, 514
PshAI GACNNNNGTC 1 cut(s) 699
PsiI TTATAA 1 cut(s) 750
Psp6I CCWGG 4 cut(s) 331, 592, 737, 826
PspGI CCWGG 4 cut(s) 331, 592, 737, 826
PspPI GGNCC 1 cut(s) 590
PspXI VCTCGAGB 1 cut(s) 218
PstI CTGCAG 1 cut(s) 868
PstNI CAGNNNCTG 1 cut(s) 872
RsaI GTAC 2 cut(s) 193, 245
RsaNI GTAC 2 cut(s) 192, 244
RseI CAYNNNNRTG 3 cut(s) 71, 311, 875
SaqAI TTAA 1 cut(s) 503
SatI GCNGC 2 cut(s) 353, 513
Sau3AI GATC 3 cut(s) 343, 834, 965
Sau96I GGNCC 1 cut(s) 590
ScrFI CCNGG 4 cut(s) 333, 594, 739, 828
SduI GDGCHC 1 cut(s) 85
SfaNI GCATC 2 cut(s) 244, 339
SfcI CTRYAG 1 cut(s) 864
Sfr274I CTCGAG 1 cut(s) 218
SfuI TTCGAA 1 cut(s) 70
SinI GGWCC 1 cut(s) 590
SlaI CTCGAG 1 cut(s) 218
SmiMI CAYNNNNRTG 3 cut(s) 71, 311, 875
SmlI CTYRAG 2 cut(s) 158, 218
SmoI CTYRAG 2 cut(s) 158, 218
SpeI ACTAGT 1 cut(s) 425
Sse9I AATT 7 cut(s) 15, 26, 123, 327, 439, 500, 744
SspI AATATT 1 cut(s) 754
SspMI CTAG 3 cut(s) 426, 935, 957
StyD4I CCNGG 4 cut(s) 331, 592, 737, 826
StyI CCWWGG 2 cut(s) 279, 776
TaaI ACNGT 1 cut(s) 874
TaiI ACGT 1 cut(s) 93
TaqI TCGA 3 cut(s) 70, 219, 890
TasI AATT 7 cut(s) 15, 26, 123, 327, 439, 500, 744
TatI WGTACW 1 cut(s) 191
TfiI GAWTC 2 cut(s) 682, 860
Tru1I TTAA 1 cut(s) 503
Tru9I TTAA 1 cut(s) 503
TscAI CASTG 2 cut(s) 792, 877
TseFI GTSAC 2 cut(s) 271, 868
TseI GCWGC 2 cut(s) 352, 512
Tsp45I GTSAC 2 cut(s) 271, 868
TspDTI ATGAA 8 cut(s) 103, 276, 305, 375, 674, 893, 938, 941
TspGWI ACGGA 1 cut(s) 52
TspRI CASTG 2 cut(s) 792, 877
Van91I CCANNNNNTGG 1 cut(s) 332
VneI GTGCAC 1 cut(s) 81
VpaK11BI GGWCC 1 cut(s) 590
XapI RAATTY 2 cut(s) 15, 744
XcmI CCANNNNNNNNNTGG 2 cut(s) 490, 1011
XhoI CTCGAG 1 cut(s) 218
XmnI GAANNNNTTC 1 cut(s) 366
XspI CTAG 3 cut(s) 426, 935, 957
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.