MD08G1024600.v1.1

dTDP-glucose 4,6-dehydratase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0011258
Physical Location & Seq
Forward (+)
1766985 .. 1769680
2696 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1024600.v1.1.491

Sequence Viewer

Length: 1032 bp
ATGGAATTGTGTAAAATATCGGGTTCCGCGGCCTTCCAATTCGGCCATCTCCCGAAACGTTCCGTCTTCCGAATGACCGGAACGGAGTTCAAACCACAGAACCGGATGTTCATCGCCGGAATGGGTTTCATCGGCCAGCGCCTGGGGCGTGAGCTCAAGAACCGCGGATGGGATGTCTCTGGTACTTGCACTAGCATTAACAAGAAGAAGAAACTTGAGGAAGAAAGGAGCTTTCAGATTTACCTTTTTGATGCAAATGAGCCAGATGTGATCATCCTTGATGCTATCAAGAACCATACGCACATGGTTGTCTCCATTCCTCCTGTTCCGGGCATCGGTGATCCGATGCTGGATTATGACCAAATTCTAAGGAGAGAACTTGTGGGTGGAAATCTTCAATGGCTTGGTTATCTGTCATCAACTAGTGTATATGGAGACACTGGCGGTGCATGGGTAGATGAGGATTATATCCCCAACCCCGAGAGTGAGTCGGGCAGGTTAAGGTTAGCTGCTGAGGAAGGATGGTTCAATTTCGGCCAGAGTCTTGGCATCTCAACACAAGTATTTCGGCTCGGAGGCATCTATGGTCCTGGTAGAAGTGCTGTCGATACAATAGTCAAGCAGGGAGCTTTATCGGAGAGTCAGAGAATGAGAGGGCATCGGCAATTCACATCAAGAGTTTCTGTTGATGATATTTGTCAAGCAATTATGGCCAGTATGTGCACACCGTCATCCAGGCGAGTTTACAATATTGTTGACGATGATCCAGCCCCAAGGGAAGAAGTATTCGAGTATGCAAGGGACTTGGTTGAGAAAAAGTGGCCTGGCTGGATCAAACAGCCTTCAGGGCAAAGAGAGTCTTCGGAACTTATTGTTAAGAAGAGAGGTTTACAGGGAGAGAAGAGAGTTTCGAATGCTCGTATGAAGAAGGAACTAGGAGTGAGGCTGCTGCATCCAAGTTACAGGTCGGGATTGCAGAGTATTATTGATCAAATGGATAGCCCTTTTCAACGTGGCAAATTGAATTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

344

Amino Acids

38.58

Weight (kDa)

9.06

Isoelectric Point (pI)

46.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 38 - 251 2.8e-06 NAD dependent epimerase/dehydratase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011258)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19690
fragaria_vesca FvH4_2g39320
malus_domestica MD08G1024600.v1.1
prunus_persica Prupe.1G374300_v2.0.a1
pyrus_communis pycom02g18430 pycom08g01950 pycom15g37000
rosa_chinensis RchiOBHm_Chr6g0301681
rosa_laevigata RLG00000011232
rosa_multiflora Rmu_sc0023062.1_g000003 Rmu_sc0034934.1_g000006 Rmu_sc0043116.1_g000002
rosa_roxburghii Rroxscaffold_7G00166530
rosa_rugosa Rorug06G0308000
rosa_samantha Rh6AG420600 Rh6BG427100 Rh6BG427200 Rh6CG433800 Rh6DG420500
rosa_wichuraiana Rw6G036390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 486
AccB7I CCANNNNNTGG 1 cut(s) 142
AccII CGCG 2 cut(s) 29, 165
AciI CCGC 5 cut(s) 27, 29, 163, 165, 444
AclI AACGTT 1 cut(s) 58
AclWI GGATC 3 cut(s) 335, 758, 839
AcoI YGGCCR 4 cut(s) 43, 133, 535, 711
AcsI RAATTY 2 cut(s) 363, 1024
AcuI CTGAAG 1 cut(s) 828
AfaI GTAC 1 cut(s) 184
AfiI CCNNNNNNNGG 4 cut(s) 142, 169, 329, 335
AgsI TTSAA 5 cut(s) 91, 398, 529, 1010, 1024
AhlI ACTAGT 1 cut(s) 422
AjnI CCWGG 4 cut(s) 141, 589, 734, 823
AloI GAACNNNNNNTCC 2 cut(s) 509, 541
AluBI AGCT 4 cut(s) 154, 231, 509, 629
AluI AGCT 4 cut(s) 154, 231, 509, 629
Alw21I GWGCWC 2 cut(s) 156, 725
Alw26I GTCTC 3 cut(s) 181, 316, 429
Alw44I GTGCAC 1 cut(s) 721
AlwI GGATC 3 cut(s) 335, 758, 839
AlwNI CAGNNNCTG 1 cut(s) 142
Ama87I CYCGRG 1 cut(s) 479
AoxI GGCC 6 cut(s) 30, 43, 133, 535, 711, 821
ApaLI GTGCAC 1 cut(s) 721
ApeKI GCWGC 3 cut(s) 509, 946, 949
ApoI RAATTY 2 cut(s) 363, 1024
AspLEI GCGC 1 cut(s) 141
AspS9I GGNCC 1 cut(s) 587
AsuC2I CCSGG 1 cut(s) 330
AsuHPI GGTGA 1 cut(s) 350
AsuII TTCGAA 1 cut(s) 911
AvaI CYCGRG 1 cut(s) 479
AvaII GGWCC 1 cut(s) 587
BaeGI GKGCMC 1 cut(s) 725
BaeI ACNNNNGTAYC 2 cut(s) 600, 633
BalI TGGCCA 1 cut(s) 713
BanII GRGCYC 1 cut(s) 156
BbsI GAAGAC 2 cut(s) 58, 852
Bbv12I GWGCWC 2 cut(s) 156, 725
BbvCI CCTCAGC 1 cut(s) 513
BbvI GCAGC 3 cut(s) 496, 933, 936
BccI CCATC 3 cut(s) 54, 162, 516
BciT130I CCWGG 4 cut(s) 143, 591, 736, 825
BclI TGATCA 2 cut(s) 270, 988
BcnI CCSGG 1 cut(s) 330
BcoDI GTCTC 3 cut(s) 181, 316, 429
BcuI ACTAGT 1 cut(s) 422
BfaI CTAG 3 cut(s) 192, 423, 935
BfoI RGCGCY 1 cut(s) 142
BfuAI ACCTGC 1 cut(s) 486
BglI GCCNNNNNGGC 1 cut(s) 847
BisI GCNGC 4 cut(s) 30, 510, 947, 950
BlsI GCNGC 4 cut(s) 31, 511, 948, 951
Bme1390I CCNGG 5 cut(s) 143, 330, 591, 736, 825
Bme18I GGWCC 1 cut(s) 587
BmeT110I CYCGRG 1 cut(s) 479
BmgT120I GGNCC 1 cut(s) 587
BmiI GGNNCC 1 cut(s) 25
BmrFI CCNGG 5 cut(s) 143, 330, 591, 736, 825
BmsI GCATC 8 cut(s) 241, 271, 336, 342, 558, 588, 667, 961
BpiI GAAGAC 2 cut(s) 58, 852
Bpu10I CCTNAGC 1 cut(s) 513
Bpu14I TTCGAA 1 cut(s) 911
BpuEI CTTGAG 2 cut(s) 140, 236
BpuMI CCSGG 1 cut(s) 330
BsaBI GATNNNNATC 1 cut(s) 110
BsaJI CCNNGG 4 cut(s) 27, 142, 163, 773
BsaWI WCCGGW 2 cut(s) 77, 102
Bsc4I CCNNNNNNNGG 4 cut(s) 142, 169, 329, 335
Bse1I ACTGG 2 cut(s) 445, 714
Bse8I GATNNNNATC 1 cut(s) 110
BseBI CCWGG 4 cut(s) 143, 591, 736, 825
BseDI CCNNGG 4 cut(s) 27, 142, 163, 773
BseGI GGATG 7 cut(s) 111, 173, 178, 273, 527, 731, 952
BseJI GATNNNNATC 1 cut(s) 110
BseLI CCNNNNNNNGG 4 cut(s) 142, 169, 329, 335
BseMII CTCAG 1 cut(s) 504
BseNI ACTGG 2 cut(s) 445, 714
BseSI GKGCMC 1 cut(s) 725
BseXI GCAGC 3 cut(s) 496, 933, 936
Bsh1236I CGCG 2 cut(s) 29, 165
BshFI GGCC 6 cut(s) 32, 45, 135, 537, 713, 823
BsiHKAI GWGCWC 2 cut(s) 156, 725
BsiHKCI CYCGRG 1 cut(s) 479
BsiSI CCGG 4 cut(s) 78, 103, 117, 329
BslFI GGGAC 1 cut(s) 815
BslI CCNNNNNNNGG 4 cut(s) 142, 169, 329, 335
BsmAI GTCTC 3 cut(s) 181, 316, 429
BsmFI GGGAC 1 cut(s) 815
BsmI GAATGC 1 cut(s) 919
BsnI GGCC 6 cut(s) 32, 45, 135, 537, 713, 823
BsoBI CYCGRG 1 cut(s) 479
Bsp119I TTCGAA 1 cut(s) 911
Bsp1286I GDGCHC 2 cut(s) 156, 725
Bsp143I GATC 5 cut(s) 270, 340, 763, 831, 988
BspACI CCGC 5 cut(s) 27, 29, 163, 165, 444
BspANI GGCC 6 cut(s) 32, 45, 135, 537, 713, 823
BspCNI CTCAG 1 cut(s) 505
BspFNI CGCG 2 cut(s) 29, 165
BspHI TCATGA 1 cut(s) 1028
BspLI GGNNCC 1 cut(s) 25
BspMI ACCTGC 1 cut(s) 486
BspPI GGATC 3 cut(s) 335, 758, 839
BspT104I TTCGAA 1 cut(s) 911
BsrI ACTGG 2 cut(s) 445, 714
BssECI CCNNGG 4 cut(s) 27, 142, 163, 773
BssMI GATC 5 cut(s) 270, 340, 763, 831, 988
BssT1I CCWWGG 1 cut(s) 773
Bst2UI CCWGG 4 cut(s) 143, 591, 736, 825
Bst4CI ACNGT 1 cut(s) 729
Bst6I CTCTTC 2 cut(s) 875, 896
BstBI TTCGAA 1 cut(s) 911
BstC8I GCNNGC 1 cut(s) 137
BstDEI CTNAG 2 cut(s) 368, 513
BstDSI CCRYGG 2 cut(s) 27, 163
BstF5I GGATG 7 cut(s) 111, 173, 178, 273, 527, 731, 952
BstFNI CGCG 2 cut(s) 29, 165
BstH2I RGCGCY 1 cut(s) 142
BstHHI GCGC 1 cut(s) 141
BstKTI GATC 5 cut(s) 273, 343, 766, 834, 991
BstMAI GTCTC 3 cut(s) 181, 316, 429
BstMBI GATC 5 cut(s) 270, 340, 763, 831, 988
BstMWI GCNNNNNNNGC 3 cut(s) 145, 710, 847
BstNI CCWGG 4 cut(s) 143, 591, 736, 825
BstSCI CCNGG 5 cut(s) 141, 328, 589, 734, 823
BstSLI GKGCMC 1 cut(s) 725
BstUI CGCG 2 cut(s) 29, 165
BstV1I GCAGC 3 cut(s) 496, 933, 936
BstV2I GAAGAC 2 cut(s) 58, 852
BstXI CCANNNNNNTGG 1 cut(s) 545
BsuRI GGCC 6 cut(s) 32, 45, 135, 537, 713, 823
BtgI CCRYGG 2 cut(s) 27, 163
BtgZI GCGATG 1 cut(s) 97
BtsCI GGATG 7 cut(s) 111, 173, 178, 273, 527, 731, 952
BtsIMutI CAGTG 1 cut(s) 438
BveI ACCTGC 1 cut(s) 486
Cac8I GCNNGC 1 cut(s) 137
CaiI CAGNNNCTG 1 cut(s) 142
CciI TCATGA 1 cut(s) 1028
CfoI GCGC 1 cut(s) 141
Cfr13I GGNCC 1 cut(s) 587
Cfr42I CCGCGG 2 cut(s) 30, 166
Csp6I GTAC 1 cut(s) 183
CviAII CATG 3 cut(s) 304, 450, 1029
CviQI GTAC 1 cut(s) 183
DdeI CTNAG 2 cut(s) 368, 513
DpnI GATC 5 cut(s) 272, 342, 765, 833, 990
DpnII GATC 5 cut(s) 270, 340, 763, 831, 988
EaeI YGGCCR 4 cut(s) 43, 133, 535, 711
Eam1104I CTCTTC 2 cut(s) 875, 896
EarI CTCTTC 2 cut(s) 875, 896
Ecl136II GAGCTC 1 cut(s) 154
Eco130I CCWWGG 1 cut(s) 773
Eco24I GRGCYC 1 cut(s) 156
Eco47I GGWCC 1 cut(s) 587
Eco53kI GAGCTC 1 cut(s) 154
Eco57I CTGAAG 1 cut(s) 828
Eco88I CYCGRG 1 cut(s) 479
EcoICRI GAGCTC 1 cut(s) 154
EcoRI GAATTC 1 cut(s) 1024
EcoRII CCWGG 4 cut(s) 141, 589, 734, 823
EcoT14I CCWWGG 1 cut(s) 773
EcoT38I GRGCYC 1 cut(s) 156
ErhI CCWWGG 1 cut(s) 773
FaeI CATG 3 cut(s) 307, 453, 1032
FalI AAGNNNNNCTT 2 cut(s) 844, 876
FaqI GGGAC 1 cut(s) 815
FatI CATG 3 cut(s) 303, 449, 1028
FbaI TGATCA 2 cut(s) 270, 988
Fnu4HI GCNGC 4 cut(s) 30, 510, 947, 950
FokI GGATG 7 cut(s) 118, 180, 185, 260, 534, 718, 939
FriOI GRGCYC 1 cut(s) 156
Fsp4HI GCNGC 4 cut(s) 30, 510, 947, 950
FspBI CTAG 3 cut(s) 192, 423, 935
GlaI GCGC 1 cut(s) 140
GluI GCNGC 4 cut(s) 30, 510, 947, 950
HaeII RGCGCY 1 cut(s) 142
HaeIII GGCC 6 cut(s) 32, 45, 135, 537, 713, 823
HapII CCGG 4 cut(s) 78, 103, 117, 329
HhaI GCGC 1 cut(s) 141
Hin1II CATG 3 cut(s) 307, 453, 1032
Hin6I GCGC 1 cut(s) 139
HinP1I GCGC 1 cut(s) 139
HincII GTYRAC 1 cut(s) 757
HindII GTYRAC 1 cut(s) 757
HinfI GANTC 4 cut(s) 488, 541, 640, 857
HpaII CCGG 4 cut(s) 78, 103, 117, 329
HphI GGTGA 1 cut(s) 350
Hpy166II GTNNAC 4 cut(s) 723, 745, 757, 890
Hpy188I TCNGA 7 cut(s) 71, 237, 345, 575, 637, 645, 865
Hpy188III TCNNGA 6 cut(s) 52, 157, 289, 675, 969, 1029
Hpy8I GTNNAC 4 cut(s) 723, 745, 757, 890
HpyAV CCTTC 4 cut(s) 43, 512, 852, 922
HpyCH4III ACNGT 1 cut(s) 729
HpyCH4IV ACGT 2 cut(s) 58, 1012
HpyCH4V TGCA 7 cut(s) 189, 254, 449, 723, 797, 952, 976
HpyF10VI GCNNNNNNNGC 3 cut(s) 145, 710, 847
HpyF3I CTNAG 2 cut(s) 368, 513
HpySE526I ACGT 2 cut(s) 58, 1012
Hsp92II CATG 3 cut(s) 307, 453, 1032
HspAI GCGC 1 cut(s) 139
Ksp22I TGATCA 2 cut(s) 270, 988
KspI CCGCGG 2 cut(s) 30, 166
Kzo9I GATC 5 cut(s) 270, 340, 763, 831, 988
LmnI GCTCC 2 cut(s) 228, 626
Lsp1109I GCAGC 3 cut(s) 496, 933, 936
LweI GCATC 8 cut(s) 241, 271, 336, 342, 558, 588, 667, 961
MaeI CTAG 3 cut(s) 192, 423, 935
MaeII ACGT 2 cut(s) 58, 1012
MaeIII GTNAC 1 cut(s) 959
MalI GATC 5 cut(s) 272, 342, 765, 833, 990
MboI GATC 5 cut(s) 270, 340, 763, 831, 988
MhlI GDGCHC 2 cut(s) 156, 725
MlsI TGGCCA 1 cut(s) 713
MluCI AATT 8 cut(s) 5, 38, 363, 529, 665, 705, 1019, 1024
MluNI TGGCCA 1 cut(s) 713
MlyI GAGTC 4 cut(s) 497, 550, 649, 866
MnlI CCTC 8 cut(s) 211, 330, 454, 508, 569, 647, 878, 936
Mox20I TGGCCA 1 cut(s) 713
MscI TGGCCA 1 cut(s) 713
MseI TTAA 3 cut(s) 198, 500, 876
Msp20I TGGCCA 1 cut(s) 713
MspA1I CMGCKG 2 cut(s) 29, 165
MspI CCGG 4 cut(s) 78, 103, 117, 329
MspR9I CCNGG 5 cut(s) 143, 330, 591, 736, 825
Mva1269I GAATGC 1 cut(s) 919
MvaI CCWGG 4 cut(s) 143, 591, 736, 825
MvnI CGCG 2 cut(s) 29, 165
MwoI GCNNNNNNNGC 3 cut(s) 145, 710, 847
NciI CCSGG 1 cut(s) 330
NdeII GATC 5 cut(s) 270, 340, 763, 831, 988
NlaIII CATG 3 cut(s) 307, 453, 1032
NlaIV GGNNCC 1 cut(s) 25
NspV TTCGAA 1 cut(s) 911
PagI TCATGA 1 cut(s) 1028
PctI GAATGC 1 cut(s) 919
PflMI CCANNNNNTGG 1 cut(s) 142
PkrI GCNGC 4 cut(s) 31, 511, 948, 951
PleI GAGTC 4 cut(s) 496, 549, 648, 865
PpsI GAGTC 4 cut(s) 496, 549, 648, 865
Psp124BI GAGCTC 1 cut(s) 156
Psp1406I AACGTT 1 cut(s) 58
Psp6I CCWGG 4 cut(s) 141, 589, 734, 823
PspGI CCWGG 4 cut(s) 141, 589, 734, 823
PspN4I GGNNCC 1 cut(s) 25
PspPI GGNCC 1 cut(s) 587
PstNI CAGNNNCTG 1 cut(s) 142
RsaI GTAC 1 cut(s) 184
RsaNI GTAC 1 cut(s) 183
SacI GAGCTC 1 cut(s) 156
SacII CCGCGG 2 cut(s) 30, 166
SaqAI TTAA 3 cut(s) 198, 500, 876
SatI GCNGC 4 cut(s) 30, 510, 947, 950
Sau3AI GATC 5 cut(s) 270, 340, 763, 831, 988
Sau96I GGNCC 1 cut(s) 587
SchI GAGTC 4 cut(s) 497, 550, 649, 866
ScrFI CCNGG 5 cut(s) 143, 330, 591, 736, 825
SduI GDGCHC 2 cut(s) 156, 725
SfaNI GCATC 8 cut(s) 241, 271, 336, 342, 558, 588, 667, 961
Sfr303I CCGCGG 2 cut(s) 30, 166
SfuI TTCGAA 1 cut(s) 911
SgrBI CCGCGG 2 cut(s) 30, 166
SinI GGWCC 1 cut(s) 587
SmlI CTYRAG 2 cut(s) 155, 215
SmoI CTYRAG 2 cut(s) 155, 215
SpeI ACTAGT 1 cut(s) 422
Sse9I AATT 8 cut(s) 5, 38, 363, 529, 665, 705, 1019, 1024
SsiI CCGC 5 cut(s) 27, 29, 163, 165, 444
SspI AATATT 1 cut(s) 751
SspMI CTAG 3 cut(s) 192, 423, 935
SstI GAGCTC 1 cut(s) 156
StyD4I CCNGG 5 cut(s) 141, 328, 589, 734, 823
StyI CCWWGG 1 cut(s) 773
TaaI ACNGT 1 cut(s) 729
TaiI ACGT 2 cut(s) 61, 1015
TaqI TCGA 3 cut(s) 606, 789, 911
TasI AATT 8 cut(s) 5, 38, 363, 529, 665, 705, 1019, 1024
TauI GCSGC 1 cut(s) 32
Tru1I TTAA 3 cut(s) 198, 500, 876
Tru9I TTAA 3 cut(s) 198, 500, 876
TscAI CASTG 1 cut(s) 445
TseI GCWGC 3 cut(s) 509, 946, 949
TspDTI ATGAA 4 cut(s) 100, 118, 938, 1017
TspGWI ACGGA 2 cut(s) 52, 98
TspRI CASTG 1 cut(s) 445
Van91I CCANNNNNTGG 1 cut(s) 142
VneI GTGCAC 1 cut(s) 721
VpaK11BI GGWCC 1 cut(s) 587
XapI RAATTY 2 cut(s) 363, 1024
XspI CTAG 3 cut(s) 192, 423, 935
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.