pycom08g01950

dTDP-glucose 4,6-dehydratase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0011258
Physical Location & Seq
Forward (+)
1431424 .. 1431979
556 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g01950.3

Sequence Viewer

Length: 405 bp
ATGATCGGAATTCTGATTTGGGTTGGTTGGGGCCCGTTATTCATAAAGTTCATCAATTGGTTGTGGGCCTTGACTGGGCTAGTAAAATCCGATTCCGGTTCAGTGTTTCTCTGTATTGAGCTCCGATATTTCGTGATGGAGTTGTGTAAAATATCGGGTTCCGCGGCCTTCCAATTCGGCCATCTCCCGAAACGTTCCGTCTTCCGTATGACCGGCACGGAGTTCAAACCACAGAACCGGATGTTCATCGCCGGAATGGGTTTCGTCGGCCAGCGCCTGGGGCGCGAGCTCAAGAACCAAGGATGGGATGTCTCTGGTACTTGCACTAGCATTAACAAGAAGAAGAAACTTGAGGAAGAAAGGAGCTTTGAGATTTACCTTTTTGATGCAAATGAGCCAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

135

Amino Acids

15.37

Weight (kDa)

9.1

Isoelectric Point (pI)

35.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011258)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19690
fragaria_vesca FvH4_2g39320
malus_domestica MD08G1024600.v1.1
prunus_persica Prupe.1G374300_v2.0.a1
pyrus_communis pycom02g18430 pycom08g01950 pycom15g37000
rosa_chinensis RchiOBHm_Chr6g0301681
rosa_laevigata RLG00000011232
rosa_multiflora Rmu_sc0023062.1_g000003 Rmu_sc0034934.1_g000006 Rmu_sc0043116.1_g000002
rosa_roxburghii Rroxscaffold_7G00166530
rosa_rugosa Rorug06G0308000
rosa_samantha Rh6AG420600 Rh6BG427100 Rh6BG427200 Rh6CG433800 Rh6DG420500
rosa_wichuraiana Rw6G036390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 277
AccII CGCG 2 cut(s) 164, 285
AciI CCGC 2 cut(s) 162, 164
AclI AACGTT 1 cut(s) 193
AcoI YGGCCR 2 cut(s) 178, 268
AcsI RAATTY 1 cut(s) 9
AfaI GTAC 1 cut(s) 319
AfiI CCNNNNNNNGG 3 cut(s) 75, 277, 304
AgsI TTSAA 1 cut(s) 226
AjnI CCWGG 1 cut(s) 276
AjuI GAANNNNNNNTTGG 1 cut(s) 33
AluBI AGCT 3 cut(s) 121, 289, 366
AluI AGCT 3 cut(s) 121, 289, 366
Alw21I GWGCWC 2 cut(s) 123, 291
Alw26I GTCTC 1 cut(s) 316
AlwNI CAGNNNCTG 1 cut(s) 277
AoxI GGCC 5 cut(s) 31, 66, 165, 178, 268
ApaI GGGCCC 1 cut(s) 35
ApoI RAATTY 1 cut(s) 9
AspLEI GCGC 2 cut(s) 276, 285
AspS9I GGNCC 3 cut(s) 31, 32, 66
BaeGI GKGCMC 1 cut(s) 35
BanII GRGCYC 3 cut(s) 35, 123, 291
BbsI GAAGAC 1 cut(s) 193
Bbv12I GWGCWC 2 cut(s) 123, 291
BccI CCATC 3 cut(s) 130, 189, 297
BciT130I CCWGG 1 cut(s) 278
BcoDI GTCTC 1 cut(s) 316
BfaI CTAG 2 cut(s) 80, 327
BfoI RGCGCY 1 cut(s) 277
BisI GCNGC 1 cut(s) 165
BlsI GCNGC 1 cut(s) 166
Bme1390I CCNGG 1 cut(s) 278
BmgT120I GGNCC 3 cut(s) 31, 32, 66
BmiI GGNNCC 3 cut(s) 32, 33, 160
BmrFI CCNGG 1 cut(s) 278
BmrI ACTGGG 1 cut(s) 84
BmsI GCATC 1 cut(s) 376
BmuI ACTGGG 1 cut(s) 84
BpiI GAAGAC 1 cut(s) 193
BpuEI CTTGAG 2 cut(s) 275, 371
BsaBI GATNNNNATC 1 cut(s) 245
BsaJI CCNNGG 3 cut(s) 162, 277, 298
BsaWI WCCGGW 2 cut(s) 95, 237
Bsc4I CCNNNNNNNGG 3 cut(s) 75, 277, 304
Bse118I RCCGGY 1 cut(s) 212
Bse1I ACTGG 1 cut(s) 79
Bse8I GATNNNNATC 1 cut(s) 245
BseBI CCWGG 1 cut(s) 278
BseDI CCNNGG 3 cut(s) 162, 277, 298
BseGI GGATG 3 cut(s) 246, 308, 313
BseJI GATNNNNATC 1 cut(s) 245
BseLI CCNNNNNNNGG 3 cut(s) 75, 277, 304
BseNI ACTGG 1 cut(s) 79
BseSI GKGCMC 1 cut(s) 35
Bsh1236I CGCG 2 cut(s) 164, 285
BshFI GGCC 5 cut(s) 33, 68, 167, 180, 270
BsiHKAI GWGCWC 2 cut(s) 123, 291
BsiSI CCGG 4 cut(s) 96, 213, 238, 252
BslI CCNNNNNNNGG 3 cut(s) 75, 277, 304
BsmAI GTCTC 1 cut(s) 316
BsnI GGCC 5 cut(s) 33, 68, 167, 180, 270
Bsp120I GGGCCC 1 cut(s) 31
Bsp1286I GDGCHC 3 cut(s) 35, 123, 291
Bsp143I GATC 1 cut(s) 3
BspACI CCGC 2 cut(s) 162, 164
BspANI GGCC 5 cut(s) 33, 68, 167, 180, 270
BspFNI CGCG 2 cut(s) 164, 285
BspLI GGNNCC 3 cut(s) 32, 33, 160
BsrFI RCCGGY 1 cut(s) 212
BsrI ACTGG 1 cut(s) 79
BssAI RCCGGY 1 cut(s) 212
BssECI CCNNGG 3 cut(s) 162, 277, 298
BssMI GATC 1 cut(s) 3
BssT1I CCWWGG 1 cut(s) 298
Bst2UI CCWGG 1 cut(s) 278
BstC8I GCNNGC 2 cut(s) 272, 287
BstDSI CCRYGG 1 cut(s) 162
BstF5I GGATG 3 cut(s) 246, 308, 313
BstFNI CGCG 2 cut(s) 164, 285
BstH2I RGCGCY 1 cut(s) 277
BstHHI GCGC 2 cut(s) 276, 285
BstKTI GATC 1 cut(s) 6
BstMAI GTCTC 1 cut(s) 316
BstMBI GATC 1 cut(s) 3
BstMWI GCNNNNNNNGC 2 cut(s) 280, 282
BstNI CCWGG 1 cut(s) 278
BstSCI CCNGG 1 cut(s) 276
BstSLI GKGCMC 1 cut(s) 35
BstUI CGCG 2 cut(s) 164, 285
BstV2I GAAGAC 1 cut(s) 193
BsuRI GGCC 5 cut(s) 33, 68, 167, 180, 270
BtgI CCRYGG 1 cut(s) 162
BtgZI GCGATG 1 cut(s) 232
BtsCI GGATG 3 cut(s) 246, 308, 313
BtsIMutI CAGTG 1 cut(s) 108
Cac8I GCNNGC 2 cut(s) 272, 287
CaiI CAGNNNCTG 1 cut(s) 277
CfoI GCGC 2 cut(s) 276, 285
Cfr10I RCCGGY 1 cut(s) 212
Cfr13I GGNCC 3 cut(s) 31, 32, 66
Cfr42I CCGCGG 1 cut(s) 165
Csp6I GTAC 1 cut(s) 318
CviQI GTAC 1 cut(s) 318
DpnI GATC 1 cut(s) 5
DpnII GATC 1 cut(s) 3
EaeI YGGCCR 2 cut(s) 178, 268
Ecl136II GAGCTC 2 cut(s) 121, 289
Eco130I CCWWGG 1 cut(s) 298
Eco24I GRGCYC 3 cut(s) 35, 123, 291
Eco53kI GAGCTC 2 cut(s) 121, 289
EcoICRI GAGCTC 2 cut(s) 121, 289
EcoO109I RGGNCCY 1 cut(s) 31
EcoRI GAATTC 1 cut(s) 9
EcoRII CCWGG 1 cut(s) 276
EcoT14I CCWWGG 1 cut(s) 298
EcoT38I GRGCYC 3 cut(s) 35, 123, 291
ErhI CCWWGG 1 cut(s) 298
FaiI YATR 2 cut(s) 44, 209
Fnu4HI GCNGC 1 cut(s) 165
FokI GGATG 3 cut(s) 253, 315, 320
FriOI GRGCYC 3 cut(s) 35, 123, 291
Fsp4HI GCNGC 1 cut(s) 165
FspBI CTAG 2 cut(s) 80, 327
GlaI GCGC 2 cut(s) 275, 284
GluI GCNGC 1 cut(s) 165
HaeII RGCGCY 1 cut(s) 277
HaeIII GGCC 5 cut(s) 33, 68, 167, 180, 270
HapII CCGG 4 cut(s) 96, 213, 238, 252
HhaI GCGC 2 cut(s) 276, 285
Hin6I GCGC 2 cut(s) 274, 283
HinP1I GCGC 2 cut(s) 274, 283
HinfI GANTC 1 cut(s) 92
HpaII CCGG 4 cut(s) 96, 213, 238, 252
Hpy188I TCNGA 4 cut(s) 8, 15, 91, 125
Hpy188III TCNNGA 3 cut(s) 133, 187, 292
Hpy99I CGWCG 1 cut(s) 269
HpyAV CCTTC 1 cut(s) 178
HpyCH4IV ACGT 1 cut(s) 193
HpyCH4V TGCA 2 cut(s) 324, 389
HpyF10VI GCNNNNNNNGC 2 cut(s) 280, 282
HpySE526I ACGT 1 cut(s) 193
HspAI GCGC 2 cut(s) 274, 283
KspI CCGCGG 1 cut(s) 165
Kzo9I GATC 1 cut(s) 3
LmnI GCTCC 2 cut(s) 126, 363
LpnPI CCDG 9 cut(s) 60, 109, 226, 251, 263, 265, 284, 290, 300
LweI GCATC 1 cut(s) 376
MaeI CTAG 2 cut(s) 80, 327
MaeII ACGT 1 cut(s) 193
MalI GATC 1 cut(s) 5
MboI GATC 1 cut(s) 3
MboII GAAGA 4 cut(s) 193, 352, 355, 368
MfeI CAATTG 1 cut(s) 55
MhlI GDGCHC 3 cut(s) 35, 123, 291
MluCI AATT 3 cut(s) 9, 55, 173
MnlI CCTC 1 cut(s) 346
MseI TTAA 1 cut(s) 333
MspA1I CMGCKG 1 cut(s) 164
MspI CCGG 4 cut(s) 96, 213, 238, 252
MspR9I CCNGG 1 cut(s) 278
MunI CAATTG 1 cut(s) 55
MvaI CCWGG 1 cut(s) 278
MvnI CGCG 2 cut(s) 164, 285
MwoI GCNNNNNNNGC 2 cut(s) 280, 282
NdeII GATC 1 cut(s) 3
NlaIV GGNNCC 3 cut(s) 32, 33, 160
PfeI GAWTC 1 cut(s) 92
PflMI CCANNNNNTGG 1 cut(s) 277
PkrI GCNGC 1 cut(s) 166
Psp124BI GAGCTC 2 cut(s) 123, 291
Psp1406I AACGTT 1 cut(s) 193
Psp6I CCWGG 1 cut(s) 276
PspGI CCWGG 1 cut(s) 276
PspN4I GGNNCC 3 cut(s) 32, 33, 160
PspOMI GGGCCC 1 cut(s) 31
PspPI GGNCC 3 cut(s) 31, 32, 66
PstNI CAGNNNCTG 1 cut(s) 277
RsaI GTAC 1 cut(s) 319
RsaNI GTAC 1 cut(s) 318
SacI GAGCTC 2 cut(s) 123, 291
SacII CCGCGG 1 cut(s) 165
SaqAI TTAA 1 cut(s) 333
SatI GCNGC 1 cut(s) 165
Sau3AI GATC 1 cut(s) 3
Sau96I GGNCC 3 cut(s) 31, 32, 66
ScrFI CCNGG 1 cut(s) 278
SduI GDGCHC 3 cut(s) 35, 123, 291
SetI ASST 5 cut(s) 123, 196, 291, 368, 381
SfaNI GCATC 1 cut(s) 376
Sfr303I CCGCGG 1 cut(s) 165
SgrBI CCGCGG 1 cut(s) 165
SmlI CTYRAG 2 cut(s) 290, 350
SmoI CTYRAG 2 cut(s) 290, 350
Sse9I AATT 3 cut(s) 9, 55, 173
SsiI CCGC 2 cut(s) 162, 164
SspMI CTAG 2 cut(s) 80, 327
SstI GAGCTC 2 cut(s) 123, 291
StyD4I CCNGG 1 cut(s) 276
StyI CCWWGG 1 cut(s) 298
TaiI ACGT 1 cut(s) 196
TasI AATT 3 cut(s) 9, 55, 173
TauI GCSGC 1 cut(s) 167
TfiI GAWTC 1 cut(s) 92
Tru1I TTAA 1 cut(s) 333
Tru9I TTAA 1 cut(s) 333
TscAI CASTG 1 cut(s) 108
TspDTI ATGAA 3 cut(s) 31, 40, 235
TspGWI ACGGA 3 cut(s) 187, 194, 233
TspRI CASTG 1 cut(s) 108
Van91I CCANNNNNTGG 1 cut(s) 277
XapI RAATTY 1 cut(s) 9
XspI CTAG 2 cut(s) 80, 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.