Rh6BG427100

dTDP-glucose 4,6-dehydratase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0011258
Physical Location & Seq
Reverse (-)
64849839 .. 64851521
1683 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG427100.1

Sequence Viewer

Length: 738 bp
ATGGATGTTATGAAGCTCCATACTCACATGGTTGTGTCAATCCCTCCAATTCCTGGTATTGGTGATCCGATGCTGCAACATGAACAACTTCTAAGGAAAGCACTTTTGGGGGGAAATCTTCAATGGCTTTGCTATTTGTCCTCAACTAGTGTATATGGAGATTGTGATGGTGCATGGGTAGATGAGGATTATCCCCCTAACCCCACAAGGGAGGTGGGAAAATTAAGGTTAGCTGCTGAGAAAGGATGGTTACACTTGGGGCAGAGTTTGGGCATCTCAACAAAAGTATTTCGGCTCGGAGGTATCTATGGTCCTGGTAGAAGTGCTGTTGACACCATAATCAAGCAGGAAGCCTTATCAAAAAGTCAGAGAATGAGAGGATATAGGCAGTTCACATCAAGGATTCATGTTGAGGACATTTGTCAAGCCGTTGAGGCCAGTATTTGCACAGCTTCCTCCAGGGAAGTTTATAATATTGTTGATGATGATCCTGCCCCAAGGGAAGCAGTGTTTGAGCATGCAAGAGACTTGCTTGACAAAAAATGGCCAGGCTGGATCAAACAGGCTTCTGAACAAAGAGAATCTACAGTAACTGTTATGAAAGATGGTTCGATGGGTGAGAAGAGAGTTTGCAATGCTCGTATGAAGAAGGAACTAGGACTTAGGCTTCTTCATCCTAGTTATAGATCAGGATTACAAAGTATTATTGACCAAATGGATAAACCATTTTCAACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

245

Amino Acids

27.48

Weight (kDa)

8.23

Isoelectric Point (pI)

34.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 39 - 159 3.4e-06 NAD dependent epimerase/dehydratase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011258)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19690
fragaria_vesca FvH4_2g39320
malus_domestica MD08G1024600.v1.1
prunus_persica Prupe.1G374300_v2.0.a1
pyrus_communis pycom02g18430 pycom08g01950 pycom15g37000
rosa_chinensis RchiOBHm_Chr6g0301681
rosa_laevigata RLG00000011232
rosa_multiflora Rmu_sc0023062.1_g000003 Rmu_sc0034934.1_g000006 Rmu_sc0043116.1_g000002
rosa_roxburghii Rroxscaffold_7G00166530
rosa_rugosa Rorug06G0308000
rosa_samantha Rh6AG420600 Rh6BG427100 Rh6BG427200 Rh6CG433800 Rh6DG420500
rosa_wichuraiana Rw6G036390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 471
AccB7I CCANNNNNTGG 1 cut(s) 53
AclWI GGATC 3 cut(s) 59, 482, 563
AcoI YGGCCR 1 cut(s) 545
AfiI CCNNNNNNNGG 3 cut(s) 53, 59, 208
AgsI TTSAA 2 cut(s) 122, 732
AhlI ACTAGT 1 cut(s) 146
AjnI CCWGG 4 cut(s) 52, 313, 458, 547
AjuI GAANNNNNNNTTGG 2 cut(s) 89, 121
AluBI AGCT 3 cut(s) 16, 233, 452
AluI AGCT 3 cut(s) 16, 233, 452
Alw26I GTCTC 1 cut(s) 519
AlwI GGATC 3 cut(s) 59, 482, 563
AlwNI CAGNNNCTG 1 cut(s) 593
AoxI GGCC 2 cut(s) 435, 545
ApeKI GCWGC 2 cut(s) 73, 233
Asp700I GAANNNNTTC 1 cut(s) 87
AspS9I GGNCC 1 cut(s) 311
AsuHPI GGTGA 2 cut(s) 74, 629
AvaII GGWCC 1 cut(s) 311
BalI TGGCCA 1 cut(s) 547
BbvI GCAGC 2 cut(s) 60, 220
BccI CCATC 4 cut(s) 161, 240, 599, 607
BceAI ACGGC 1 cut(s) 413
BciT130I CCWGG 4 cut(s) 54, 315, 460, 549
BcoDI GTCTC 1 cut(s) 519
BcuI ACTAGT 1 cut(s) 146
BfaI CTAG 3 cut(s) 147, 656, 678
BfmI CTRYAG 1 cut(s) 585
BglI GCCNNNNNGGC 1 cut(s) 434
BisI GCNGC 2 cut(s) 74, 234
BlsI GCNGC 2 cut(s) 75, 235
Bme1390I CCNGG 4 cut(s) 54, 315, 460, 549
Bme18I GGWCC 1 cut(s) 311
BmgT120I GGNCC 1 cut(s) 311
BmrFI CCNGG 4 cut(s) 54, 315, 460, 549
BmsI GCATC 2 cut(s) 60, 282
BoxI GACNNNNGTC 1 cut(s) 420
BpmI CTGGAG 1 cut(s) 442
BsaBI GATNNNNATC 1 cut(s) 486
BsaJI CCNNGG 2 cut(s) 459, 497
Bsc4I CCNNNNNNNGG 3 cut(s) 53, 59, 208
Bse1I ACTGG 1 cut(s) 438
Bse3DI GCAATG 1 cut(s) 640
Bse8I GATNNNNATC 1 cut(s) 486
BseBI CCWGG 4 cut(s) 54, 315, 460, 549
BseDI CCNNGG 2 cut(s) 459, 497
BseGI GGATG 3 cut(s) 10, 251, 673
BseJI GATNNNNATC 1 cut(s) 486
BseLI CCNNNNNNNGG 3 cut(s) 53, 59, 208
BseMI GCAATG 1 cut(s) 640
BseMII CTCAG 1 cut(s) 228
BseNI ACTGG 1 cut(s) 438
BseXI GCAGC 2 cut(s) 60, 220
BshFI GGCC 2 cut(s) 437, 547
BslI CCNNNNNNNGG 3 cut(s) 53, 59, 208
BsmAI GTCTC 1 cut(s) 519
BsnI GGCC 2 cut(s) 437, 547
Bsp143I GATC 4 cut(s) 64, 487, 555, 686
BspANI GGCC 2 cut(s) 437, 547
BspCNI CTCAG 1 cut(s) 229
BspPI GGATC 3 cut(s) 59, 482, 563
BsrDI GCAATG 1 cut(s) 640
BsrI ACTGG 1 cut(s) 438
BssECI CCNNGG 2 cut(s) 459, 497
BssMI GATC 4 cut(s) 64, 487, 555, 686
BssT1I CCWWGG 1 cut(s) 497
Bst2UI CCWGG 4 cut(s) 54, 315, 460, 549
Bst4CI ACNGT 2 cut(s) 589, 595
Bst6I CTCTTC 1 cut(s) 617
BstC8I GCNNGC 1 cut(s) 519
BstDEI CTNAG 3 cut(s) 92, 237, 662
BstF5I GGATG 3 cut(s) 10, 251, 673
BstKTI GATC 4 cut(s) 67, 490, 558, 689
BstMAI GTCTC 1 cut(s) 519
BstMBI GATC 4 cut(s) 64, 487, 555, 686
BstMWI GCNNNNNNNGC 1 cut(s) 434
BstNI CCWGG 4 cut(s) 54, 315, 460, 549
BstNSI RCATGY 1 cut(s) 521
BstPAI GACNNNNGTC 1 cut(s) 420
BstSCI CCNGG 4 cut(s) 52, 313, 458, 547
BstSFI CTRYAG 1 cut(s) 585
BstV1I GCAGC 2 cut(s) 60, 220
BsuRI GGCC 2 cut(s) 437, 547
BtsCI GGATG 3 cut(s) 10, 251, 673
BtsI GCAGTG 1 cut(s) 513
BtsIMutI CAGTG 1 cut(s) 513
Cac8I GCNNGC 1 cut(s) 519
CaiI CAGNNNCTG 1 cut(s) 593
Cfr13I GGNCC 1 cut(s) 311
CspCI CAANNNNNGTGG 2 cut(s) 195, 230
CviAII CATG 5 cut(s) 28, 80, 174, 407, 518
DdeI CTNAG 3 cut(s) 92, 237, 662
DpnI GATC 4 cut(s) 66, 489, 557, 688
DpnII GATC 4 cut(s) 64, 487, 555, 686
EaeI YGGCCR 1 cut(s) 545
Eam1104I CTCTTC 1 cut(s) 617
EarI CTCTTC 1 cut(s) 617
Eco130I CCWWGG 1 cut(s) 497
Eco47I GGWCC 1 cut(s) 311
EcoRII CCWGG 4 cut(s) 52, 313, 458, 547
EcoT14I CCWWGG 1 cut(s) 497
ErhI CCWWGG 1 cut(s) 497
FaeI CATG 5 cut(s) 31, 83, 177, 410, 521
FatI CATG 5 cut(s) 27, 79, 173, 406, 517
Fnu4HI GCNGC 2 cut(s) 74, 234
FokI GGATG 3 cut(s) 17, 258, 660
Fsp4HI GCNGC 2 cut(s) 74, 234
FspBI CTAG 3 cut(s) 147, 656, 678
GluI GCNGC 2 cut(s) 74, 234
GsuI CTGGAG 1 cut(s) 442
HaeIII GGCC 2 cut(s) 437, 547
Hin1II CATG 5 cut(s) 31, 83, 177, 410, 521
HincII GTYRAC 1 cut(s) 331
HindII GTYRAC 1 cut(s) 331
HinfI GANTC 2 cut(s) 403, 581
HphI GGTGA 2 cut(s) 74, 629
Hpy166II GTNNAC 2 cut(s) 331, 393
Hpy188I TCNGA 4 cut(s) 69, 299, 369, 571
Hpy188III TCNNGA 1 cut(s) 690
Hpy8I GTNNAC 2 cut(s) 331, 393
HpyAV CCTTC 1 cut(s) 643
HpyCH4III ACNGT 2 cut(s) 589, 595
HpyCH4V TGCA 5 cut(s) 76, 173, 447, 521, 633
HpyF10VI GCNNNNNNNGC 1 cut(s) 434
HpyF3I CTNAG 3 cut(s) 92, 237, 662
Hsp92II CATG 5 cut(s) 31, 83, 177, 410, 521
Kzo9I GATC 4 cut(s) 64, 487, 555, 686
LmnI GCTCC 1 cut(s) 21
Lsp1109I GCAGC 2 cut(s) 60, 220
LweI GCATC 2 cut(s) 60, 282
MaeI CTAG 3 cut(s) 147, 656, 678
MaeIII GTNAC 2 cut(s) 249, 589
MalI GATC 4 cut(s) 66, 489, 557, 688
MboI GATC 4 cut(s) 64, 487, 555, 686
MboII GAAGA 4 cut(s) 110, 634, 658, 662
MlsI TGGCCA 1 cut(s) 547
MluCI AATT 2 cut(s) 48, 221
MluNI TGGCCA 1 cut(s) 547
MnlI CCTC 9 cut(s) 54, 151, 178, 205, 293, 371, 406, 427, 466
Mox20I TGGCCA 1 cut(s) 547
MroXI GAANNNNTTC 1 cut(s) 87
MscI TGGCCA 1 cut(s) 547
MseI TTAA 1 cut(s) 224
MslI CAYNNNNRTG 1 cut(s) 32
Msp20I TGGCCA 1 cut(s) 547
MspR9I CCNGG 4 cut(s) 54, 315, 460, 549
MvaI CCWGG 4 cut(s) 54, 315, 460, 549
MwoI GCNNNNNNNGC 1 cut(s) 434
NdeII GATC 4 cut(s) 64, 487, 555, 686
NlaIII CATG 5 cut(s) 31, 83, 177, 410, 521
NspI RCATGY 1 cut(s) 521
PaeI GCATGC 1 cut(s) 521
PdmI GAANNNNTTC 1 cut(s) 87
PfeI GAWTC 2 cut(s) 403, 581
PflMI CCANNNNNTGG 1 cut(s) 53
PkrI GCNGC 2 cut(s) 75, 235
PshAI GACNNNNGTC 1 cut(s) 420
PsiI TTATAA 1 cut(s) 471
Psp6I CCWGG 4 cut(s) 52, 313, 458, 547
PspGI CCWGG 4 cut(s) 52, 313, 458, 547
PspPI GGNCC 1 cut(s) 311
PstNI CAGNNNCTG 1 cut(s) 593
RseI CAYNNNNRTG 1 cut(s) 32
SaqAI TTAA 1 cut(s) 224
SatI GCNGC 2 cut(s) 74, 234
Sau3AI GATC 4 cut(s) 64, 487, 555, 686
Sau96I GGNCC 1 cut(s) 311
ScrFI CCNGG 4 cut(s) 54, 315, 460, 549
SetI ASST 6 cut(s) 18, 216, 230, 235, 304, 454
SfaNI GCATC 2 cut(s) 60, 282
SfcI CTRYAG 1 cut(s) 585
SinI GGWCC 1 cut(s) 311
SmiMI CAYNNNNRTG 1 cut(s) 32
SpeI ACTAGT 1 cut(s) 146
SphI GCATGC 1 cut(s) 521
Sse9I AATT 2 cut(s) 48, 221
SspI AATATT 1 cut(s) 475
SspMI CTAG 3 cut(s) 147, 656, 678
StyD4I CCNGG 4 cut(s) 52, 313, 458, 547
StyI CCWWGG 1 cut(s) 497
TaaI ACNGT 2 cut(s) 589, 595
TaqI TCGA 1 cut(s) 611
TasI AATT 2 cut(s) 48, 221
TfiI GAWTC 2 cut(s) 403, 581
Tru1I TTAA 1 cut(s) 224
Tru9I TTAA 1 cut(s) 224
TscAI CASTG 1 cut(s) 513
TseI GCWGC 2 cut(s) 73, 233
TspDTI ATGAA 6 cut(s) 26, 96, 395, 614, 659, 662
TspRI CASTG 1 cut(s) 513
Van91I CCANNNNNTGG 1 cut(s) 53
VpaK11BI GGWCC 1 cut(s) 311
XceI RCATGY 1 cut(s) 521
XcmI CCANNNNNNNNNTGG 1 cut(s) 211
XmnI GAANNNNTTC 1 cut(s) 87
XspI CTAG 3 cut(s) 147, 656, 678
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.