FvH4_2g40230

Belongs to the MT-A70-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0010167
Physical Location & Seq
Reverse (-)
28700937 .. 28703498
2562 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g40230.t2

Sequence Viewer

Length: 1233 bp
ATGAAGGACTCCAAAACCACCGATAAGCTCACCGAGTTTTTGAAATCCGGGATTTACCGGTTCGAAGATTCCAACGCCGTTTTCATCGACCCGATCCGAGTGCTCAACCGCTCCTACTCCCGGTTTAGGGTTTCACCTTCCGCTTACTACTGTCGCCGATTCGAATCCACGTGTCCGCCGGAGCCTACCGTCGCTTCAAGTTCCAGAAAGCGGAAGCGGAAGGAGAAGAAGAAGCCTCAGGCTCTCAACGACAGAGAACAAGTCGCCGATCGACGCCACCAGGAAGTGAGAGCTTTGTTAGTGAAGGCACATGAATCTTTGATGAGATCAAGTGAGGTGTTAGAGGTTATGAGCAATTTGAGAGGTTCTGAAACTTGGTCTCCAGGTGTTGAACAGTCGTTTGTTGAACTCGCATTGGTTTGGCAGGCTCCTCTTTACGAGATTAGTCTGAATTACAAAGCAGGGGAAGATGGAGGCTCGCATGCCACGCAAGACTGTGAGCAATGTGTTCTCCCTGTGTTTAACAACTTGGTCGTAAATGAGAGCAGTAATGATGTGGAGGCTGAAGTTTTGAAGAGTCGATATATTTTGCCGCCAAAGAGTTCCTTCTACATGTCAGATATGGGGCAGATTCGCAATCTAATTCCTGCTGAGTCTGATTGTGGCTTCAGTGTTATAGTTGTTGATCCACCTTGGGAAAATGGCAGTGCTCGACAAAAGTTGAGGTACAAAACTTTGCCCAACCATTATTTCTTATCCATTCCGATCAAGCAACTTAGTCACACAGATGGAGCACTTGTTGCATTATGGGTAACAAATAGAGAGAAGTTGCGTGATTTTGTTGAGAAAGAGCTGTTTCCTGCTTGGGGAGTCACACATGTTGCTACTTTTTTCTGGTTGAAGGTGAAAGCTGATGGTTCCTTGATTTGTGATTTAGACCTATTCCATCATAGGCCATATGAGTGCCTTCTTGTGGGCATCTATCATGGGAAGGTTATGAACTTTGAACAGTTATTGGGATCAGATCATATACCAGATAATCAAATTATATTAAGTGTACCGGGGGATTACTCAAGGAAGCCCCCAATTGGAGGATTGTTACAGGAGTTTGCTCCCAGGCTCAAAGGTGGTCGATGCATTGAACTATTTGCTAGAGAAATGACATCCGGATGGGTTTCTTGGGGAAACGAACCCCTTCATTTTCAGGAATCCAGGAATTTTGTAATCGAATAG

Protein Analysis

411

Amino Acids

46.88

Weight (kDa)

7.16

Isoelectric Point (pI)

49.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MT-A70 PF05063 223 - 329 7.8e-23 MT-A70
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 111
AccIII TCCGGA 1 cut(s) 1166
AciI CCGC 6 cut(s) 109, 141, 176, 211, 217, 593
AclWI GGATC 3 cut(s) 88, 680, 1027
AcsI RAATTY 1 cut(s) 1216
AcuI CTGAAG 2 cut(s) 585, 652
AcvI CACGTG 1 cut(s) 171
AcyI GRCGYC 1 cut(s) 274
AfaI GTAC 2 cut(s) 728, 1059
AfiI CCNNNNNNNGG 8 cut(s) 120, 126, 127, 210, 866, 973, 1088, 1091
AflIII ACRYGT 3 cut(s) 170, 612, 877
AgeI ACCGGT 1 cut(s) 57
AgsI TTSAA 8 cut(s) 43, 198, 392, 407, 574, 901, 1007, 1142
AjnI CCWGG 4 cut(s) 279, 382, 1115, 1211
AjuI GAANNNNNNNTTGG 4 cut(s) 65, 97, 734, 766
AluBI AGCT 4 cut(s) 28, 293, 853, 911
AluI AGCT 4 cut(s) 28, 293, 853, 911
Alw21I GWGCWC 3 cut(s) 105, 712, 796
Alw26I GTCTC 1 cut(s) 384
AlwI GGATC 3 cut(s) 88, 680, 1027
Aor13HI TCCGGA 1 cut(s) 1166
AoxI GGCC 1 cut(s) 953
ApoI RAATTY 1 cut(s) 1216
AsiGI ACCGGT 1 cut(s) 57
Asp700I GAANNNNTTC 1 cut(s) 1194
AsuC2I CCSGG 3 cut(s) 49, 121, 1062
AsuHPI GGTGA 3 cut(s) 22, 126, 916
AsuII TTCGAA 2 cut(s) 63, 162
AxyI CCTNAGG 1 cut(s) 237
BbrPI CACGTG 1 cut(s) 171
Bbv12I GWGCWC 3 cut(s) 105, 712, 796
BccI CCATC 5 cut(s) 464, 782, 908, 954, 1164
BceAI ACGGC 1 cut(s) 62
BcgI CGANNNNNNTGC 2 cut(s) 82, 116
BciT130I CCWGG 4 cut(s) 281, 384, 1117, 1213
BcnI CCSGG 3 cut(s) 49, 121, 1062
BcoDI GTCTC 1 cut(s) 384
BfaI CTAG 1 cut(s) 1152
BisI GCNGC 1 cut(s) 593
BlsI GCNGC 1 cut(s) 594
Bme1390I CCNGG 7 cut(s) 49, 121, 281, 384, 1062, 1117, 1213
BmiI GGNNCC 3 cut(s) 183, 429, 919
BmrFI CCNGG 7 cut(s) 49, 121, 281, 384, 1062, 1117, 1213
BmsI GCATC 2 cut(s) 987, 1124
BpmI CTGGAG 1 cut(s) 366
Bpu14I TTCGAA 2 cut(s) 63, 162
BpuEI CTTGAG 1 cut(s) 1057
BpuMI CCSGG 3 cut(s) 49, 121, 1062
BsaAI YACGTR 1 cut(s) 171
BsaBI GATNNNNATC 1 cut(s) 163
BsaHI GRCGYC 1 cut(s) 274
BsaI GGTCTC 1 cut(s) 384
BsaJI CCNNGG 3 cut(s) 692, 1061, 1115
BsaWI WCCGGW 2 cut(s) 57, 1166
BsaXI ACNNNNNCTCC 4 cut(s) 364, 394, 783, 813
Bsc4I CCNNNNNNNGG 8 cut(s) 120, 126, 127, 210, 866, 973, 1088, 1091
Bse118I RCCGGY 1 cut(s) 57
Bse21I CCTNAGG 1 cut(s) 237
Bse3DI GCAATG 1 cut(s) 509
Bse8I GATNNNNATC 1 cut(s) 163
BseAI TCCGGA 1 cut(s) 1166
BseBI CCWGG 4 cut(s) 281, 384, 1117, 1213
BseDI CCNNGG 3 cut(s) 692, 1061, 1115
BseGI GGATG 2 cut(s) 1163, 1175
BseJI GATNNNNATC 1 cut(s) 163
BseLI CCNNNNNNNGG 8 cut(s) 120, 126, 127, 210, 866, 973, 1088, 1091
BseMI GCAATG 1 cut(s) 509
BseMII CTCAG 2 cut(s) 251, 642
BseRI GAGGAG 1 cut(s) 420
Bsh1285I CGRYCG 1 cut(s) 271
BshFI GGCC 1 cut(s) 955
BshTI ACCGGT 1 cut(s) 57
BsiEI CGRYCG 1 cut(s) 271
BsiHKAI GWGCWC 3 cut(s) 105, 712, 796
BsiSI CCGG 6 cut(s) 48, 58, 121, 179, 1061, 1167
BslI CCNNNNNNNGG 8 cut(s) 120, 126, 127, 210, 866, 973, 1088, 1091
BsmAI GTCTC 1 cut(s) 384
BsnI GGCC 1 cut(s) 955
Bso31I GGTCTC 1 cut(s) 384
Bsp119I TTCGAA 2 cut(s) 63, 162
Bsp1286I GDGCHC 3 cut(s) 105, 712, 796
Bsp13I TCCGGA 1 cut(s) 1166
Bsp143I GATC 7 cut(s) 93, 268, 326, 685, 765, 1019, 1024
BspACI CCGC 6 cut(s) 109, 141, 176, 211, 217, 593
BspANI GGCC 1 cut(s) 955
BspCNI CTCAG 2 cut(s) 250, 643
BspEI TCCGGA 1 cut(s) 1166
BspLI GGNNCC 3 cut(s) 183, 429, 919
BspPI GGATC 3 cut(s) 88, 680, 1027
BspT104I TTCGAA 2 cut(s) 63, 162
BspTNI GGTCTC 1 cut(s) 384
BsrBI CCGCTC 1 cut(s) 111
BsrDI GCAATG 1 cut(s) 509
BsrFI RCCGGY 1 cut(s) 57
BssAI RCCGGY 1 cut(s) 57
BssECI CCNNGG 3 cut(s) 692, 1061, 1115
BssMI GATC 7 cut(s) 93, 268, 326, 685, 765, 1019, 1024
BssNI GRCGYC 1 cut(s) 274
BssT1I CCWWGG 1 cut(s) 692
Bst2UI CCWGG 4 cut(s) 281, 384, 1117, 1213
Bst4CI ACNGT 5 cut(s) 152, 190, 396, 497, 1011
Bst6I CTCTTC 1 cut(s) 569
BstACI GRCGYC 1 cut(s) 274
BstAPI GCANNNNNTGC 1 cut(s) 800
BstBAI YACGTR 1 cut(s) 171
BstBI TTCGAA 2 cut(s) 63, 162
BstC8I GCNNGC 3 cut(s) 426, 479, 483
BstDEI CTNAG 3 cut(s) 237, 651, 776
BstF5I GGATG 2 cut(s) 1163, 1175
BstKTI GATC 7 cut(s) 96, 271, 329, 688, 768, 1022, 1027
BstMAI GTCTC 1 cut(s) 384
BstMBI GATC 7 cut(s) 93, 268, 326, 685, 765, 1019, 1024
BstMCI CGRYCG 1 cut(s) 271
BstMWI GCNNNNNNNGC 2 cut(s) 487, 800
BstNI CCWGG 4 cut(s) 281, 384, 1117, 1213
BstNSI RCATGY 3 cut(s) 485, 616, 881
BstSCI CCNGG 7 cut(s) 47, 119, 279, 382, 1060, 1115, 1211
Bsu36I CCTNAGG 1 cut(s) 237
BsuRI GGCC 1 cut(s) 955
BtsCI GGATG 2 cut(s) 1163, 1175
BtsI GCAGTG 1 cut(s) 712
BtsIMutI CAGTG 2 cut(s) 676, 712
Cac8I GCNNGC 3 cut(s) 426, 479, 483
Cfr10I RCCGGY 1 cut(s) 57
CseI GACGC 1 cut(s) 282
Csp6I GTAC 2 cut(s) 727, 1058
CspAI ACCGGT 1 cut(s) 57
CviAII CATG 5 cut(s) 311, 482, 613, 878, 986
CviQI GTAC 2 cut(s) 727, 1058
DdeI CTNAG 3 cut(s) 237, 651, 776
DpnI GATC 7 cut(s) 95, 270, 328, 687, 767, 1021, 1026
DpnII GATC 7 cut(s) 93, 268, 326, 685, 765, 1019, 1024
Eam1104I CTCTTC 1 cut(s) 569
EarI CTCTTC 1 cut(s) 569
EciI GGCGGA 1 cut(s) 165
Eco130I CCWWGG 1 cut(s) 692
Eco31I GGTCTC 1 cut(s) 384
Eco57I CTGAAG 2 cut(s) 585, 652
Eco72I CACGTG 1 cut(s) 171
Eco81I CCTNAGG 1 cut(s) 237
EcoRII CCWGG 4 cut(s) 279, 382, 1115, 1211
EcoT14I CCWWGG 1 cut(s) 692
EcoT22I ATGCAT 1 cut(s) 1139
ErhI CCWWGG 1 cut(s) 692
FaeI CATG 5 cut(s) 314, 485, 616, 881, 989
FalI AAGNNNNNCTT 2 cut(s) 590, 622
FatI CATG 5 cut(s) 310, 481, 612, 877, 985
FauNDI CATATG 1 cut(s) 958
Fnu4HI GCNGC 1 cut(s) 593
FokI GGATG 2 cut(s) 1150, 1182
Fsp4HI GCNGC 1 cut(s) 593
FspBI CTAG 1 cut(s) 1152
GluI GCNGC 1 cut(s) 593
GsuI CTGGAG 1 cut(s) 366
HaeIII GGCC 1 cut(s) 955
HapII CCGG 6 cut(s) 48, 58, 121, 179, 1061, 1167
HgaI GACGC 1 cut(s) 282
Hin1I GRCGYC 1 cut(s) 274
Hin1II CATG 5 cut(s) 314, 485, 616, 881, 989
HpaII CCGG 6 cut(s) 48, 58, 121, 179, 1061, 1167
HphI GGTGA 3 cut(s) 22, 126, 916
Hpy166II GTNNAC 1 cut(s) 1058
Hpy188I TCNGA 7 cut(s) 98, 370, 450, 619, 658, 765, 1024
Hpy188III TCNNGA 3 cut(s) 204, 1167, 1205
Hpy8I GTNNAC 1 cut(s) 1058
Hpy99I CGWCG 2 cut(s) 194, 276
HpyAV CCTTC 8 cut(s) 147, 214, 298, 616, 895, 977, 985, 1205
HpyCH4III ACNGT 5 cut(s) 152, 190, 396, 497, 1011
HpyCH4IV ACGT 1 cut(s) 170
HpyCH4V TGCA 2 cut(s) 803, 1137
HpyF10VI GCNNNNNNNGC 2 cut(s) 487, 800
HpyF3I CTNAG 3 cut(s) 237, 651, 776
HpySE526I ACGT 1 cut(s) 170
Hsp92I GRCGYC 1 cut(s) 274
Hsp92II CATG 5 cut(s) 314, 485, 616, 881, 989
Kpn2I TCCGGA 1 cut(s) 1166
Kzo9I GATC 7 cut(s) 93, 268, 326, 685, 765, 1019, 1024
LmnI GCTCC 5 cut(s) 116, 181, 433, 791, 1117
LweI GCATC 2 cut(s) 987, 1124
MaeI CTAG 1 cut(s) 1152
MaeII ACGT 1 cut(s) 170
MaeIII GTNAC 4 cut(s) 779, 811, 871, 1098
MalI GATC 7 cut(s) 95, 270, 328, 687, 767, 1021, 1026
MbiI CCGCTC 1 cut(s) 111
MboI GATC 7 cut(s) 93, 268, 326, 685, 765, 1019, 1024
MboII GAAGA 5 cut(s) 77, 238, 241, 479, 586
MfeI CAATTG 1 cut(s) 1086
MhlI GDGCHC 3 cut(s) 105, 712, 796
MluCI AATT 6 cut(s) 355, 451, 642, 1044, 1086, 1216
MlyI GAGTC 4 cut(s) 2, 586, 662, 879
MmeI TCCRAC 1 cut(s) 96
MnlI CCTC 9 cut(s) 246, 328, 337, 356, 441, 467, 553, 717, 1085
Mph1103I ATGCAT 1 cut(s) 1139
MroI TCCGGA 1 cut(s) 1166
MroXI GAANNNNTTC 1 cut(s) 1194
MseI TTAA 2 cut(s) 522, 1052
MslI CAYNNNNRTG 3 cut(s) 786, 961, 1168
MspI CCGG 6 cut(s) 48, 58, 121, 179, 1061, 1167
MspR9I CCNGG 7 cut(s) 49, 121, 281, 384, 1062, 1117, 1213
MunI CAATTG 1 cut(s) 1086
MvaI CCWGG 4 cut(s) 281, 384, 1117, 1213
MwoI GCNNNNNNNGC 2 cut(s) 487, 800
NciI CCSGG 3 cut(s) 49, 121, 1062
NdeI CATATG 1 cut(s) 958
NdeII GATC 7 cut(s) 93, 268, 326, 685, 765, 1019, 1024
NlaIII CATG 5 cut(s) 314, 485, 616, 881, 989
NlaIV GGNNCC 3 cut(s) 183, 429, 919
NmuCI GTSAC 2 cut(s) 779, 871
NsiI ATGCAT 1 cut(s) 1139
NspI RCATGY 3 cut(s) 485, 616, 881
NspV TTCGAA 2 cut(s) 63, 162
PaeI GCATGC 1 cut(s) 485
PciI ACATGT 2 cut(s) 612, 877
PdmI GAANNNNTTC 1 cut(s) 1194
PfeI GAWTC 6 cut(s) 68, 159, 164, 314, 631, 1208
PfoI TCCNGGA 2 cut(s) 47, 1211
PinAI ACCGGT 1 cut(s) 57
PkrI GCNGC 1 cut(s) 594
Ple19I CGATCG 1 cut(s) 271
PleI GAGTC 4 cut(s) 2, 585, 661, 878
PmaCI CACGTG 1 cut(s) 171
PmlI CACGTG 1 cut(s) 171
PpsI GAGTC 4 cut(s) 2, 585, 661, 878
Ppu21I YACGTR 1 cut(s) 171
PscI ACATGT 2 cut(s) 612, 877
Psp6I CCWGG 4 cut(s) 279, 382, 1115, 1211
PspCI CACGTG 1 cut(s) 171
PspGI CCWGG 4 cut(s) 279, 382, 1115, 1211
PspN4I GGNNCC 3 cut(s) 183, 429, 919
PvuI CGATCG 1 cut(s) 271
RsaI GTAC 2 cut(s) 728, 1059
RsaNI GTAC 2 cut(s) 727, 1058
RseI CAYNNNNRTG 3 cut(s) 786, 961, 1168
SaqAI TTAA 2 cut(s) 522, 1052
SatI GCNGC 1 cut(s) 593
Sau3AI GATC 7 cut(s) 93, 268, 326, 685, 765, 1019, 1024
SchI GAGTC 4 cut(s) 2, 586, 662, 879
ScrFI CCNGG 7 cut(s) 49, 121, 281, 384, 1062, 1117, 1213
SduI GDGCHC 3 cut(s) 105, 712, 796
SfaNI GCATC 2 cut(s) 987, 1124
SfuI TTCGAA 2 cut(s) 63, 162
SmiMI CAYNNNNRTG 3 cut(s) 786, 961, 1168
SmlI CTYRAG 1 cut(s) 1072
SmoI CTYRAG 1 cut(s) 1072
SphI GCATGC 1 cut(s) 485
Sse9I AATT 6 cut(s) 355, 451, 642, 1044, 1086, 1216
SsiI CCGC 6 cut(s) 109, 141, 176, 211, 217, 593
SspMI CTAG 1 cut(s) 1152
StyD4I CCNGG 7 cut(s) 47, 119, 279, 382, 1060, 1115, 1211
StyI CCWWGG 1 cut(s) 692
TaaI ACNGT 5 cut(s) 152, 190, 396, 497, 1011
TaiI ACGT 1 cut(s) 173
TaqI TCGA 8 cut(s) 63, 87, 162, 271, 580, 712, 1132, 1227
TasI AATT 6 cut(s) 355, 451, 642, 1044, 1086, 1216
TauI GCSGC 1 cut(s) 595
TfiI GAWTC 6 cut(s) 68, 159, 164, 314, 631, 1208
Tru1I TTAA 2 cut(s) 522, 1052
Tru9I TTAA 2 cut(s) 522, 1052
TscAI CASTG 2 cut(s) 676, 712
TseFI GTSAC 2 cut(s) 779, 871
Tsp45I GTSAC 2 cut(s) 779, 871
TspDTI ATGAA 5 cut(s) 17, 73, 327, 1013, 1187
TspRI CASTG 2 cut(s) 676, 712
XapI RAATTY 1 cut(s) 1216
XceI RCATGY 3 cut(s) 485, 616, 881
XmnI GAANNNNTTC 1 cut(s) 1194
XspI CTAG 1 cut(s) 1152
Zsp2I ATGCAT 1 cut(s) 1139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.