Rroxscaffold_7G00167690

Belongs to the MT-A70-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0010167
Physical Location & Seq
Reverse (-)
8739798 .. 8742881
3084 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00167690.1

Sequence Viewer

Length: 1254 bp
ATGGAGTACTCCAAAACCACCGATAAGCTAACCGAGTTTTTAGACTCCGGGATTTACCGGTTCGAAGATTCCAGCGCCGTTTTCATCGACCCGGTTCGCGTGCTCAACCGCTCCTACACCCGGTTTAGGGTTTCACCTTCCGCTTACTACTGTCGCCGATTCGACACCACGTGCCCACCGGAGCCTAGCGTCGCTTCGAATTCCAGAAAGCGAAAGCGGAAGGAGAAGAAGAAGCCTCGGGCTCTGAACGAAAGAGAACAAGTCGCCGATCAACGCCACCAGGAAGTGAGACCTTTCTTACTGAAGGCACATGAATCTTTGATCCGAACAAGTGAGGTGTTAGAGGTTATGAGCAATTTGAGGGGTTGTTTGGGTCCTGAAACTTCCTCTCCAGGTGTTGAACAGTCATTTGTTGAACTCGCAAGGGTCTGGCAGGCTCCTCTTTACGAGATTACTCTAAACTTCCATAAAAATTACAAAGAAGGGGAAGATGGAGGCTCCCTTGCTACGCAAGACTGTGAACAATGTGTTCTCCCTGTGTTTAACAACTTGGTCGTTAATGAGAGCAGTAATGATGTGGAGGCTGAAGTTTTGACCAGTCGATACGTTTTGCCTCCGAAGAGTTCCTTCTACATGTCAGATATGGGGCAGATTCACAATCTAATTCCTGCTGAGTCTGATTTTGGCTTCAGTGTTATAGTTGTTGATCCACCATGGGAAAATGGCAGTGCTCGGCAGAAGTTGAGGTACAAAACTTTGCCCAACCGATATTTCTTATCCATTCCGATCAAGCAACTTTGTCACACAGATGGAGCATTTGTCGCCTTATGGGTAACAAATAGGGAGAAGTTGCGTGATTTTGTCGAGAAAGAGCTGTTTCCTGCTTGGGGAGTCACACATGTTGCTACTTTTTTCTGGTTGAAGGTGAAAGCTGACGGTTCCTTGATTTGTGATTTAGACCTGTTTCATCATAGGCCATATGAGTGCCTTCTTGTGGGCATCTGTCATGGGAAGGTTATGAATTCTAAACAGCTATTGGGATCAGATCATATACCAGATAATCAAATTATATTAAGCGTACCGGGGGATTACTCAAGGAAGCCCCCAATAGGAGGATTGTTACAGGAGCTTGCTCCGAGGCTCAAAGGTGGTCGATCCATTGAACTATTTGCTAGAGAAATGACAACCGGATGGGTTTCTTGGGGGAATGAACCCCTTCATTTTCAAGAATCCAGGAATTTTGTAACCGAATAG

Protein Analysis

417

Amino Acids

47.67

Weight (kDa)

6.84

Isoelectric Point (pI)

48.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MT-A70 PF05063 230 - 404 2.7e-35 MT-A70
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 111
AccII CGCG 1 cut(s) 99
AciI CCGC 3 cut(s) 109, 141, 217
AclWI GGATC 4 cut(s) 316, 701, 1048, 1149
AcsI RAATTY 3 cut(s) 199, 1021, 1237
AcuI CTGAAG 3 cut(s) 323, 606, 673
AcvI CACGTG 1 cut(s) 171
AdeI CACNNNGTG 1 cut(s) 171
AfaI GTAC 3 cut(s) 8, 749, 1080
AfiI CCNNNNNNNGG 7 cut(s) 120, 126, 127, 887, 994, 1109, 1112
AflIII ACRYGT 2 cut(s) 633, 898
AgeI ACCGGT 1 cut(s) 57
AgsI TTSAA 5 cut(s) 401, 416, 922, 1163, 1226
AjnI CCWGG 3 cut(s) 279, 391, 1232
AjuI GAANNNNNNNTTGG 2 cut(s) 755, 787
AluBI AGCT 5 cut(s) 28, 874, 932, 1033, 1129
AluI AGCT 5 cut(s) 28, 874, 932, 1033, 1129
Alw21I GWGCWC 2 cut(s) 105, 733
Alw26I GTCTC 1 cut(s) 283
AlwI GGATC 4 cut(s) 316, 701, 1048, 1149
Ama87I CYCGRG 1 cut(s) 237
AoxI GGCC 1 cut(s) 974
ApoI RAATTY 3 cut(s) 199, 1021, 1237
AsiGI ACCGGT 1 cut(s) 57
Asp700I GAANNNNTTC 1 cut(s) 1215
AspLEI GCGC 1 cut(s) 77
AspS9I GGNCC 1 cut(s) 374
AsuC2I CCSGG 4 cut(s) 49, 92, 121, 1083
AsuHPI GGTGA 2 cut(s) 126, 937
AsuII TTCGAA 2 cut(s) 63, 197
AvaI CYCGRG 1 cut(s) 237
AvaII GGWCC 1 cut(s) 374
BaeGI GKGCMC 1 cut(s) 176
BanII GRGCYC 1 cut(s) 244
BarI GAAGNNNNNNTAC 2 cut(s) 731, 763
BbrPI CACGTG 1 cut(s) 171
Bbv12I GWGCWC 2 cut(s) 105, 733
BccI CCATC 3 cut(s) 485, 803, 1185
BceAI ACGGC 1 cut(s) 62
BciT130I CCWGG 3 cut(s) 281, 393, 1234
BcnI CCSGG 4 cut(s) 49, 92, 121, 1083
BcoDI GTCTC 1 cut(s) 283
BfaI CTAG 2 cut(s) 186, 1173
BfoI RGCGCY 1 cut(s) 78
BmcAI AGTACT 1 cut(s) 8
Bme1390I CCNGG 7 cut(s) 49, 92, 121, 281, 393, 1083, 1234
Bme18I GGWCC 1 cut(s) 374
BmeT110I CYCGRG 1 cut(s) 237
BmgT120I GGNCC 1 cut(s) 374
BmiI GGNNCC 5 cut(s) 183, 375, 438, 499, 940
BmrFI CCNGG 7 cut(s) 49, 92, 121, 281, 393, 1083, 1234
BmsI GCATC 1 cut(s) 1008
BpmI CTGGAG 1 cut(s) 375
Bpu14I TTCGAA 2 cut(s) 63, 197
BpuEI CTTGAG 1 cut(s) 1078
BpuMI CCSGG 4 cut(s) 49, 92, 121, 1083
BsaAI YACGTR 1 cut(s) 171
BsaI GGTCTC 1 cut(s) 283
BsaJI CCNNGG 4 cut(s) 236, 713, 1082, 1136
BsaWI WCCGGW 3 cut(s) 57, 178, 1187
BsaXI ACNNNNNCTCC 4 cut(s) 373, 403, 804, 834
Bsc4I CCNNNNNNNGG 7 cut(s) 120, 126, 127, 887, 994, 1109, 1112
Bse118I RCCGGY 1 cut(s) 57
Bse1I ACTGG 1 cut(s) 597
BseBI CCWGG 3 cut(s) 281, 393, 1234
BseDI CCNNGG 4 cut(s) 236, 713, 1082, 1136
BseGI GGATG 1 cut(s) 1196
BseLI CCNNNNNNNGG 7 cut(s) 120, 126, 127, 887, 994, 1109, 1112
BseMII CTCAG 1 cut(s) 663
BseNI ACTGG 1 cut(s) 597
BseRI GAGGAG 1 cut(s) 429
BseSI GKGCMC 1 cut(s) 176
Bsh1236I CGCG 1 cut(s) 99
BshFI GGCC 1 cut(s) 976
BshTI ACCGGT 1 cut(s) 57
BsiHKAI GWGCWC 2 cut(s) 105, 733
BsiHKCI CYCGRG 1 cut(s) 237
BsiSI CCGG 7 cut(s) 48, 58, 92, 121, 179, 1082, 1188
BslI CCNNNNNNNGG 7 cut(s) 120, 126, 127, 887, 994, 1109, 1112
BsmAI GTCTC 1 cut(s) 283
BsnI GGCC 1 cut(s) 976
Bso31I GGTCTC 1 cut(s) 283
BsoBI CYCGRG 1 cut(s) 237
Bsp119I TTCGAA 2 cut(s) 63, 197
Bsp1286I GDGCHC 4 cut(s) 105, 176, 244, 733
Bsp143I GATC 7 cut(s) 268, 321, 706, 786, 1040, 1045, 1154
Bsp19I CCATGG 1 cut(s) 713
BspACI CCGC 3 cut(s) 109, 141, 217
BspANI GGCC 1 cut(s) 976
BspCNI CTCAG 1 cut(s) 664
BspFNI CGCG 1 cut(s) 99
BspLI GGNNCC 5 cut(s) 183, 375, 438, 499, 940
BspPI GGATC 4 cut(s) 316, 701, 1048, 1149
BspT104I TTCGAA 2 cut(s) 63, 197
BspTNI GGTCTC 1 cut(s) 283
BsrBI CCGCTC 1 cut(s) 111
BsrFI RCCGGY 1 cut(s) 57
BsrI ACTGG 1 cut(s) 597
BssAI RCCGGY 1 cut(s) 57
BssECI CCNNGG 4 cut(s) 236, 713, 1082, 1136
BssMI GATC 7 cut(s) 268, 321, 706, 786, 1040, 1045, 1154
BssT1I CCWWGG 1 cut(s) 713
Bst2UI CCWGG 3 cut(s) 281, 393, 1234
Bst4CI ACNGT 4 cut(s) 152, 405, 518, 938
Bst6I CTCTTC 1 cut(s) 614
BstBAI YACGTR 1 cut(s) 171
BstBI TTCGAA 2 cut(s) 63, 197
BstC8I GCNNGC 3 cut(s) 101, 435, 1131
BstDEI CTNAG 1 cut(s) 672
BstDSI CCRYGG 1 cut(s) 713
BstF5I GGATG 1 cut(s) 1196
BstFNI CGCG 1 cut(s) 99
BstH2I RGCGCY 1 cut(s) 78
BstHHI GCGC 1 cut(s) 77
BstKTI GATC 7 cut(s) 271, 324, 709, 789, 1043, 1048, 1157
BstMAI GTCTC 1 cut(s) 283
BstMBI GATC 7 cut(s) 268, 321, 706, 786, 1040, 1045, 1154
BstMWI GCNNNNNNNGC 1 cut(s) 821
BstNI CCWGG 3 cut(s) 281, 393, 1234
BstNSI RCATGY 2 cut(s) 637, 902
BstSCI CCNGG 7 cut(s) 47, 90, 119, 279, 391, 1081, 1232
BstSLI GKGCMC 1 cut(s) 176
BstUI CGCG 1 cut(s) 99
BsuRI GGCC 1 cut(s) 976
BtgI CCRYGG 1 cut(s) 713
BtsCI GGATG 1 cut(s) 1196
BtsI GCAGTG 1 cut(s) 733
BtsIMutI CAGTG 2 cut(s) 697, 733
Cac8I GCNNGC 3 cut(s) 101, 435, 1131
CfoI GCGC 1 cut(s) 77
Cfr10I RCCGGY 1 cut(s) 57
Cfr13I GGNCC 1 cut(s) 374
CseI GACGC 1 cut(s) 178
Csp6I GTAC 3 cut(s) 7, 748, 1079
CspAI ACCGGT 1 cut(s) 57
CviAII CATG 5 cut(s) 311, 634, 714, 899, 1007
CviQI GTAC 3 cut(s) 7, 748, 1079
DdeI CTNAG 1 cut(s) 672
DpnI GATC 7 cut(s) 270, 323, 708, 788, 1042, 1047, 1156
DpnII GATC 7 cut(s) 268, 321, 706, 786, 1040, 1045, 1154
DraIII CACNNNGTG 1 cut(s) 171
Eam1104I CTCTTC 1 cut(s) 614
EarI CTCTTC 1 cut(s) 614
Eco130I CCWWGG 1 cut(s) 713
Eco24I GRGCYC 1 cut(s) 244
Eco31I GGTCTC 1 cut(s) 283
Eco47I GGWCC 1 cut(s) 374
Eco57I CTGAAG 3 cut(s) 323, 606, 673
Eco72I CACGTG 1 cut(s) 171
Eco88I CYCGRG 1 cut(s) 237
EcoO109I RGGNCCY 1 cut(s) 374
EcoRI GAATTC 2 cut(s) 199, 1021
EcoRII CCWGG 3 cut(s) 279, 391, 1232
EcoT14I CCWWGG 1 cut(s) 713
EcoT38I GRGCYC 1 cut(s) 244
ErhI CCWWGG 1 cut(s) 713
FaeI CATG 5 cut(s) 314, 637, 717, 902, 1010
FalI AAGNNNNNCTT 2 cut(s) 611, 643
FatI CATG 5 cut(s) 310, 633, 713, 898, 1006
FauNDI CATATG 1 cut(s) 979
FokI GGATG 1 cut(s) 1203
FriOI GRGCYC 1 cut(s) 244
FspBI CTAG 2 cut(s) 186, 1173
GlaI GCGC 1 cut(s) 76
GsuI CTGGAG 1 cut(s) 375
HaeII RGCGCY 1 cut(s) 78
HaeIII GGCC 1 cut(s) 976
HapII CCGG 7 cut(s) 48, 58, 92, 121, 179, 1082, 1188
HgaI GACGC 1 cut(s) 178
HhaI GCGC 1 cut(s) 77
Hin1II CATG 5 cut(s) 314, 637, 717, 902, 1010
Hin6I GCGC 1 cut(s) 75
HinP1I GCGC 1 cut(s) 75
HinfI GANTC 8 cut(s) 44, 68, 159, 314, 652, 674, 891, 1229
HpaII CCGG 7 cut(s) 48, 58, 92, 121, 179, 1082, 1188
HphI GGTGA 2 cut(s) 126, 937
Hpy166II GTNNAC 1 cut(s) 521
Hpy188I TCNGA 8 cut(s) 246, 326, 618, 640, 679, 786, 1045, 1137
Hpy188III TCNNGA 4 cut(s) 204, 377, 865, 1226
Hpy8I GTNNAC 1 cut(s) 521
Hpy99I CGWCG 1 cut(s) 194
HpyAV CCTTC 9 cut(s) 147, 214, 298, 476, 637, 916, 998, 1006, 1226
HpyCH4III ACNGT 4 cut(s) 152, 405, 518, 938
HpyCH4IV ACGT 2 cut(s) 170, 606
HpyF10VI GCNNNNNNNGC 1 cut(s) 821
HpyF3I CTNAG 1 cut(s) 672
HpySE526I ACGT 2 cut(s) 170, 606
Hsp92II CATG 5 cut(s) 314, 637, 717, 902, 1010
HspAI GCGC 1 cut(s) 75
Kzo9I GATC 7 cut(s) 268, 321, 706, 786, 1040, 1045, 1154
LmnI GCTCC 7 cut(s) 116, 181, 442, 503, 812, 1126, 1138
LweI GCATC 1 cut(s) 1008
MaeI CTAG 2 cut(s) 186, 1173
MaeII ACGT 2 cut(s) 170, 606
MaeIII GTNAC 5 cut(s) 800, 832, 892, 1119, 1243
MalI GATC 7 cut(s) 270, 323, 708, 788, 1042, 1047, 1156
MbiI CCGCTC 1 cut(s) 111
MboI GATC 7 cut(s) 268, 321, 706, 786, 1040, 1045, 1154
MboII GAAGA 5 cut(s) 77, 238, 241, 500, 631
MhlI GDGCHC 4 cut(s) 105, 176, 244, 733
MluCI AATT 7 cut(s) 199, 355, 472, 663, 1021, 1065, 1237
MlyI GAGTC 3 cut(s) 38, 683, 900
MroXI GAANNNNTTC 1 cut(s) 1215
MseI TTAA 3 cut(s) 543, 558, 1073
MslI CAYNNNNRTG 2 cut(s) 807, 982
MspI CCGG 7 cut(s) 48, 58, 92, 121, 179, 1082, 1188
MspR9I CCNGG 7 cut(s) 49, 92, 121, 281, 393, 1083, 1234
MvaI CCWGG 3 cut(s) 281, 393, 1234
MvnI CGCG 1 cut(s) 99
MwoI GCNNNNNNNGC 1 cut(s) 821
NciI CCSGG 4 cut(s) 49, 92, 121, 1083
NcoI CCATGG 1 cut(s) 713
NdeI CATATG 1 cut(s) 979
NdeII GATC 7 cut(s) 268, 321, 706, 786, 1040, 1045, 1154
NlaIII CATG 5 cut(s) 314, 637, 717, 902, 1010
NlaIV GGNNCC 5 cut(s) 183, 375, 438, 499, 940
NmeAIII GCCGAG 1 cut(s) 712
NmuCI GTSAC 2 cut(s) 800, 892
NspI RCATGY 2 cut(s) 637, 902
NspV TTCGAA 2 cut(s) 63, 197
PciI ACATGT 2 cut(s) 633, 898
PdmI GAANNNNTTC 1 cut(s) 1215
PfeI GAWTC 5 cut(s) 68, 159, 314, 652, 1229
PfoI TCCNGGA 2 cut(s) 47, 1232
PinAI ACCGGT 1 cut(s) 57
PleI GAGTC 3 cut(s) 38, 682, 899
PmaCI CACGTG 1 cut(s) 171
PmlI CACGTG 1 cut(s) 171
PpsI GAGTC 3 cut(s) 38, 682, 899
Ppu21I YACGTR 1 cut(s) 171
PpuMI RGGWCCY 1 cut(s) 374
PscI ACATGT 2 cut(s) 633, 898
Psp5II RGGWCCY 1 cut(s) 374
Psp6I CCWGG 3 cut(s) 279, 391, 1232
PspCI CACGTG 1 cut(s) 171
PspGI CCWGG 3 cut(s) 279, 391, 1232
PspN4I GGNNCC 5 cut(s) 183, 375, 438, 499, 940
PspPI GGNCC 1 cut(s) 374
PspPPI RGGWCCY 1 cut(s) 374
RsaI GTAC 3 cut(s) 8, 749, 1080
RsaNI GTAC 3 cut(s) 7, 748, 1079
RseI CAYNNNNRTG 2 cut(s) 807, 982
SaqAI TTAA 3 cut(s) 543, 558, 1073
Sau3AI GATC 7 cut(s) 268, 321, 706, 786, 1040, 1045, 1154
Sau96I GGNCC 1 cut(s) 374
ScaI AGTACT 1 cut(s) 8
SchI GAGTC 3 cut(s) 38, 683, 900
ScrFI CCNGG 7 cut(s) 49, 92, 121, 281, 393, 1083, 1234
SduI GDGCHC 4 cut(s) 105, 176, 244, 733
SfaNI GCATC 1 cut(s) 1008
SfuI TTCGAA 2 cut(s) 63, 197
SinI GGWCC 1 cut(s) 374
SmiMI CAYNNNNRTG 2 cut(s) 807, 982
SmlI CTYRAG 1 cut(s) 1093
SmoI CTYRAG 1 cut(s) 1093
Sse9I AATT 7 cut(s) 199, 355, 472, 663, 1021, 1065, 1237
SsiI CCGC 3 cut(s) 109, 141, 217
SspMI CTAG 2 cut(s) 186, 1173
StyD4I CCNGG 7 cut(s) 47, 90, 119, 279, 391, 1081, 1232
StyI CCWWGG 1 cut(s) 713
TaaI ACNGT 4 cut(s) 152, 405, 518, 938
TaiI ACGT 2 cut(s) 173, 609
TaqI TCGA 7 cut(s) 63, 87, 162, 197, 601, 864, 1153
TasI AATT 7 cut(s) 199, 355, 472, 663, 1021, 1065, 1237
TatI WGTACW 1 cut(s) 6
TfiI GAWTC 5 cut(s) 68, 159, 314, 652, 1229
Tru1I TTAA 3 cut(s) 543, 558, 1073
Tru9I TTAA 3 cut(s) 543, 558, 1073
TscAI CASTG 2 cut(s) 697, 733
TseFI GTSAC 2 cut(s) 800, 892
Tsp45I GTSAC 2 cut(s) 800, 892
TspDTI ATGAA 6 cut(s) 73, 327, 956, 1034, 1208, 1224
TspRI CASTG 2 cut(s) 697, 733
VpaK11BI GGWCC 1 cut(s) 374
XapI RAATTY 3 cut(s) 199, 1021, 1237
XceI RCATGY 2 cut(s) 637, 902
XmnI GAANNNNTTC 1 cut(s) 1215
XspI CTAG 2 cut(s) 186, 1173
ZrmI AGTACT 1 cut(s) 8
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.