Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0004023

Physical Location & Seq

Genomic Coordinates Forward (+)

29228974 .. 29234852

Gene Structure 5879 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g41260.t3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2298 bp

ATGGCGTCTCACATTGTTGGATACCCTCGTATGGGCCCCAAGAGAGAGCTCAAGTTTGCCTTGGAATCTTTCTGGGATGGCAAGAGCAGTGCCGAAGATTTGCAAAAGGTGTCTGCTGATCTCAGGTGTTCCATCTGGAAGCAGATGGCTGATGCCGGGATCAAGCACATTCCCAGCAACACCTTCTCTCACTACGATCAGGTGCTTGACACCACCGCATTGCTAGGGGCTGTTCCACCAAGATATGGTTGGAATGGTGGTGAGATTGGATTTGACACTTACTTCTCCATGGCCAGAGGAAATGCCTCTGTTCCTGCTATGGAAATGACCAAGTGGTTCGACACCAACTACCATTTCATTGTCCCTGAGTTGGGTCCTGACGTCAAATTCTCATATGCTTCTCACAAGGCCGTTAAGGAATACAAGGAGGCTAAAGCGCTTGGAGTGGATACAGTCCCGGTACTTGTCGGCCCAGTGTCCTACTTGTTGCTGTCCAAACCAGCGAAGGGTGTTGAAAAGACATTTTCGCTTCTCTCTCTTCTAGGAAAGATCATTCCTATTTACAAGGAGGTTATATCTGAGCTCAAGGCAGCTGGAGCTTCATGGATTCAGTTTGATGAGCCCACACTTGTCATGGATCTTGATTCTCACAAGTTGCACGCATTTACTGATGCTTACTCTCAGTTAGAGTCAACTCTATCCGGCTTGAATGTTCTTATTGAGACTTACTTTGCTGATGTACCTGCTGAGGCATTCAAGACTCTTACTTCTTTGAAGGGTGTCACTGCCTATGGTTTTGATTTAGTTCGTGGTACTAAGACCCTTGACTTGATTAAGAGTGACTTCCCTAAAGGCAAGTACCTCTTTGCTGGATTGGTTGATGGAAGGAACATTTGGGCAAATGATCTTTCTGCTTCCCTCAGTACATTGCAGGCTCTTGAAGGCATTGTTGGCAAAGACCACCTTGTCGTCTCTACCTCCTGCTCTCTTCTTCACACTGCTGTTGATCTTGTTAATGAGACTAAGCTGGACAATGAAATCAAATCGTGGCTTGCCTTTGCTGCCCAGAAAATTGTTGAAGTTAATGCTTTGGCAAAGGCATTGGCCGGTCAAAAGGATGAGGCATTTTTCTCAAGCAATGCTGCAGCTCAGGCTTCAAGGAAGTCGTCCCCAAGGGTGACCAATGAGGCTGTGCAAAAGGCTGCTGCGGCTTTGAAGGGTTCTGACCACCGTCGTGCTACTAATGTAAGTGCCAGACTTGATGCTCAACAGAAGAAGCTTAATCTTCCAGTTCTCCCAACTACCACCATTGGATCATTCCCTCAGACCATTGAACTCAGAAGGGTCCGCCGTGAATACAAGGCCAAAAAGATTTCTGAGGAGGAGTATGTCAAGGCCATTAAGGAGGAAATTAGCAAAGTTGTTAAGCTTCAGGAGGAGCTTGACATTGATGTCCTTGTTCATGGGGAGCCTGAGAGAAACGACATGGTTGAGTACTTTGGAGAGCAATTATCAGGGTTTGCCTTTACTGTCAATGGGTGGGTGCAATCTTATGGATCTCGATGTGTGAAACCACCAATCATCTATGGTGATGTGAGCCGCCCAAAGCCAATGACTGTATTCTGGTCATCTACTGCTCAGAGTATGACTGCTCGCCCAATGAAGGGAATGCTTACAGGCCCTGTTACCATCCTCAACTGGTCCTTTGTTAGAAATGACCAGCCCAGGTCTGAGACTACCTACCAGATTGCTTTGGCCATCAAGGATGAAGTGGAGGATCTTGAGAAAGCCGGTATCAATGTTATTCAAATTGATGAGGCTGCATTAAGAGAAGGGTTGCCTCTCAGGAAGTCTGAGCAAGCTCACTACTTGGACTGGGCTGTCCACTCATTCAGGATCACTAACGTTGGTGTCCAGGACACTACTCAGATTCACACTCACATGTGCTACTCCAACTTCAACGATATCATCCACTCAATCATCGACATGGATGCTGATGTGATCACCATTGAGAATTCTCGCTCTGATGAGAAGCTCCTGTCAGTCTTCCGAGAGGGAGTGAAGTATGGTGCTGGTATTGGTCCGGGTGTTTATGACATCCACTCTCCAAGAATACCATCAACTGAGGAGATTGCTGACCGGATTAACAAGATGCTTGCGGTCCTCGAGACAAATATCTTGTGGGTCAACCCTGACTGTGGGCTAAAGACTCGCAAGTACGCTGAGGTGAAACCAGCTCTTAGTGCTATGGTTGCTGCTGCCAAGCAACTCCGCACTCAGCTTGCCAGTGCTAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000096 GO:0000097 GO:0003674 GO:0003824 GO:0003871 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005777 GO:0005829 GO:0005886 GO:0005911 GO:0006082 GO:0006520 GO:0006555 GO:0006790 GO:0006807 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0008168 GO:0008172 GO:0008270 GO:0008652 GO:0008705 GO:0009058 GO:0009066 GO:0009067 GO:0009086 GO:0009506 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009651 GO:0009941 GO:0009987 GO:0010035 GO:0010038 GO:0010043 GO:0016020 GO:0016053 GO:0016740 GO:0016741 GO:0019752 GO:0030054 GO:0031090 GO:0031967 GO:0031975 GO:0032259 GO:0042084 GO:0042085 GO:0042221 GO:0042579 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044272 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046686 GO:0046872 GO:0046914 GO:0048046 GO:0050667 GO:0050896 GO:0055044 GO:0071704 GO:0071944 GO:0098588 GO:0098805 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00270 ko00450 ko01100 ko01110 ko01230 map00270 map00450 map01100 map01110 map01230

Pfam Domains

Protein Families

Meth_synt_1 Meth_synt_2

Protein Analysis

766

Amino Acids

84.58

Weight (kDa)

6.53

Isoelectric Point (pI)

33.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Meth_synt_1	PF08267	3 - 315	7.8e-116	Cobalamin-independent synthase, N-terminal domain
Meth_synt_2	PF01717	432 - 755	9.9e-160	Cobalamin-independent synthase, Catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0004023)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G03780 AT3G03780 AT3G03780 AT5G17920 AT5G17920
fragaria_vesca	FvH4_1g28390 FvH4_1g28390 FvH4_1g28390 FvH4_1g28390 FvH4_2g41260 FvH4_2g41260 FvH4_2g41260 FvH4_2g41260 FvH4_2g41260 FvH4_2g41260
malus_domestica	MD12G1008100.v1.1 MD14G1005500.v1.1
prunus_persica	Prupe.7G009200_v2.0.a1 Prupe.7G009200_v2.0.a1 Prupe.7G009200_v2.0.a1
pyrus_communis	pycom12g00610 pycom14g00410
rosa_chinensis	RchiOBHm_Chr3g0496511
rosa_laevigata	RLG00000022522
rosa_multiflora	Rmu_sc0000620.1_g000008
rosa_roxburghii	Rroxscaffold_6G00389060
rosa_rugosa	Rorug03G0283400 Rorug03G0283500
rosa_samantha	Rh3AG329600 Rh3BG365100 Rh3CG362500 Rh3DG365600
rosa_wichuraiana	Rw3G028830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	3 cut(s)	965, 1451, 1880
AatII	GACGTC	1 cut(s)	384
Acc36I	ACCTGC	1 cut(s)	751
AccB7I	CCANNNNNTGG	1 cut(s)	245
AciI	CCGC	6 cut(s)	216, 1208, 1348, 1600, 2159, 2272
AclI	AACGTT	1 cut(s)	1905
AclWI	GGATC	6 cut(s)	167, 645, 1321, 1564, 1785, 1904
AcoI	YGGCCR	3 cut(s)	291, 1104, 1755
AcsI	RAATTY	2 cut(s)	386, 2014
AcuI	CTGAAG	1 cut(s)	1415
AcyI	GRCGYC	2 cut(s)	5, 381
AfaI	GTAC	7 cut(s)	462, 741, 814, 860, 925, 1496, 2219
AfeI	AGCGCT	1 cut(s)	438
AfiI	CCNNNNNNNGG	9 cut(s)	31, 32, 245, 370, 371, 506, 1663, 1664, 2198
AflIII	ACRYGT	1 cut(s)	1941
AjnI	CCWGG	2 cut(s)	1724, 1914
AjuI	GAANNNNNNNTTGG	6 cut(s)	338, 370, 877, 909, 933, 965
AleI	CACNNNNGTG	1 cut(s)	1233
Alw21I	GWGCWC	2 cut(s)	51, 585
Alw26I	GTCTC	6 cut(s)	12, 716, 976, 1013, 1727, 2162
AlwI	GGATC	6 cut(s)	167, 645, 1321, 1564, 1785, 1904
AlwNI	CAGNNNCTG	1 cut(s)	1682
Ama87I	CYCGRG	1 cut(s)	2165
Aor51HI	AGCGCT	1 cut(s)	438
AoxI	GGCC	9 cut(s)	34, 291, 408, 469, 1104, 1362, 1395, 1678, 1755
ApaI	GGGCCC	1 cut(s)	38
ApeKI	GCWGC	9 cut(s)	590, 1061, 1142, 1145, 1202, 1205, 1820, 2255, 2258
ApoI	RAATTY	2 cut(s)	386, 2014
AspLEI	GCGC	1 cut(s)	439
AspS9I	GGNCC	9 cut(s)	34, 35, 374, 470, 1345, 1679, 1701, 2081, 2161
AsuC2I	CCSGG	3 cut(s)	157, 458, 2085
AsuHPI	GGTGA	5 cut(s)	272, 1189, 1601, 1996, 2239
AvaI	CYCGRG	1 cut(s)	2165
AvaII	GGWCC	5 cut(s)	374, 1345, 1701, 2081, 2161
BaeGI	GKGCMC	1 cut(s)	38
BaeI	ACNNNNGTAYC	2 cut(s)	13, 46
BalI	TGGCCA	2 cut(s)	293, 1757
BanII	GRGCYC	4 cut(s)	38, 51, 585, 624
BbsI	GAAGAC	1 cut(s)	2038
Bbv12I	GWGCWC	2 cut(s)	51, 585
BbvCI	CCTCAGC	2 cut(s)	747, 2223
BbvI	GCAGC	9 cut(s)	602, 1048, 1129, 1157, 1189, 1192, 1807, 2242, 2245
BccI	CCATC	7 cut(s)	71, 139, 140, 875, 1697, 1766, 2125
BceAI	ACGGC	2 cut(s)	395, 1335
BcgI	CGANNNNNNTGC	2 cut(s)	1973, 2007
BciT130I	CCWGG	2 cut(s)	1726, 1916
BciVI	GTATCC	2 cut(s)	14, 442
BclI	TGATCA	1 cut(s)	2001
BcnI	CCSGG	3 cut(s)	157, 458, 2085
BcoDI	GTCTC	6 cut(s)	12, 716, 976, 1013, 1727, 2162
BfaI	CTAG	2 cut(s)	224, 542
BfmI	CTRYAG	1 cut(s)	1143
BfoI	RGCGCY	1 cut(s)	440
BfuAI	ACCTGC	1 cut(s)	751
BfuI	GTATCC	2 cut(s)	14, 442
BmcAI	AGTACT	1 cut(s)	1496
Bme1390I	CCNGG	5 cut(s)	157, 458, 1726, 1916, 2085
Bme18I	GGWCC	5 cut(s)	374, 1345, 1701, 2081, 2161
BmeT110I	CYCGRG	1 cut(s)	2165
BmgT120I	GGNCC	9 cut(s)	34, 35, 374, 470, 1345, 1679, 1701, 2081, 2161
BmiI	GGNNCC	5 cut(s)	36, 37, 375, 1346, 1470
BmrFI	CCNGG	5 cut(s)	157, 458, 1726, 1916, 2085
BmrI	ACTGGG	2 cut(s)	467, 1885
BmsI	GCATC	5 cut(s)	142, 661, 1252, 1981, 2142
BmuI	ACTGGG	2 cut(s)	467, 1885
BoxI	GACNNNNGTC	1 cut(s)	1230
BpiI	GAAGAC	1 cut(s)	2038
BpmI	CTGGAG	1 cut(s)	615
Bpu10I	CCTNAGC	3 cut(s)	747, 1149, 2223
BpuEI	CTTGAG	4 cut(s)	35, 569, 1117, 1802
BpuMI	CCSGG	3 cut(s)	157, 458, 2085
BsaHI	GRCGYC	2 cut(s)	5, 381
BsaJI	CCNNGG	4 cut(s)	60, 288, 1172, 1724
BsaWI	WCCGGW	1 cut(s)	2139
Bsc4I	CCNNNNNNNGG	9 cut(s)	31, 32, 245, 370, 371, 506, 1663, 1664, 2198
Bse118I	RCCGGY	2 cut(s)	1106, 1790
Bse1I	ACTGG	5 cut(s)	473, 1289, 1703, 1880, 2286
Bse3DI	GCAATG	3 cut(s)	218, 926, 1144
BseBI	CCWGG	2 cut(s)	1726, 1916
BseDI	CCNNGG	4 cut(s)	60, 288, 1172, 1724
BseGI	GGATG	7 cut(s)	82, 1123, 1689, 1771, 1968, 1996, 2097
BseLI	CCNNNNNNNGG	9 cut(s)	31, 32, 245, 370, 371, 506, 1663, 1664, 2198
BseMI	GCAATG	3 cut(s)	218, 926, 1144
BseNI	ACTGG	5 cut(s)	473, 1289, 1703, 1880, 2286
BseRI	GAGGAG	4 cut(s)	1394, 1397, 1451, 2141
BseSI	GKGCMC	1 cut(s)	38
BseXI	GCAGC	9 cut(s)	602, 1048, 1129, 1157, 1189, 1192, 1807, 2242, 2245
BseYI	CCCAGC	1 cut(s)	173
BshFI	GGCC	9 cut(s)	36, 293, 410, 471, 1106, 1364, 1397, 1680, 1757
BsiHKAI	GWGCWC	2 cut(s)	51, 585
BsiHKCI	CYCGRG	1 cut(s)	2165
BsiSI	CCGG	7 cut(s)	156, 458, 702, 1107, 1791, 2084, 2140
BslFI	GGGAC	3 cut(s)	347, 440, 1153
BslI	CCNNNNNNNGG	9 cut(s)	31, 32, 245, 370, 371, 506, 1663, 1664, 2198
BsmAI	GTCTC	6 cut(s)	12, 716, 976, 1013, 1727, 2162
BsmBI	CGTCTC	2 cut(s)	12, 976
BsmFI	GGGAC	3 cut(s)	347, 440, 1153
BsmI	GAATGC	2 cut(s)	752, 1674
BsnI	GGCC	9 cut(s)	36, 293, 410, 471, 1106, 1364, 1397, 1680, 1757
BsoBI	CYCGRG	1 cut(s)	2165
Bsp120I	GGGCCC	1 cut(s)	34
Bsp1286I	GDGCHC	4 cut(s)	38, 51, 585, 624
Bsp19I	CCATGG	1 cut(s)	288
BspACI	CCGC	6 cut(s)	216, 1208, 1348, 1600, 2159, 2272
BspANI	GGCC	9 cut(s)	36, 293, 410, 471, 1106, 1364, 1397, 1680, 1757
BspLI	GGNNCC	5 cut(s)	36, 37, 375, 1346, 1470
BspMAI	CTGCAG	1 cut(s)	1147
BspMI	ACCTGC	1 cut(s)	751
BspPI	GGATC	6 cut(s)	167, 645, 1321, 1564, 1785, 1904
BsrDI	GCAATG	3 cut(s)	218, 926, 1144
BsrFI	RCCGGY	2 cut(s)	1106, 1790
BsrI	ACTGG	5 cut(s)	473, 1289, 1703, 1880, 2286
BssAI	RCCGGY	2 cut(s)	1106, 1790
BssECI	CCNNGG	4 cut(s)	60, 288, 1172, 1724
BssNI	GRCGYC	2 cut(s)	5, 381
BssT1I	CCWWGG	3 cut(s)	60, 288, 1172
Bst2UI	CCWGG	2 cut(s)	1726, 1916
Bst4CI	ACNGT	5 cut(s)	454, 1232, 1531, 1618, 2198
Bst6I	CTCTTC	2 cut(s)	543, 993
BstACI	GRCGYC	2 cut(s)	5, 381
BstC8I	GCNNGC	7 cut(s)	660, 933, 1053, 1654, 1860, 2157, 2283
BstDSI	CCRYGG	1 cut(s)	288
BstEII	GGTNACC	1 cut(s)	1177
BstF5I	GGATG	7 cut(s)	82, 1123, 1689, 1771, 1968, 1996, 2097
BstH2I	RGCGCY	1 cut(s)	440
BstHHI	GCGC	1 cut(s)	439
BstMAI	GTCTC	6 cut(s)	12, 716, 976, 1013, 1727, 2162
BstMWI	GCNNNNNNNGC	7 cut(s)	596, 951, 1061, 1151, 1208, 2243, 2252
BstNI	CCWGG	2 cut(s)	1726, 1916
BstNSI	RCATGY	1 cut(s)	1945
BstPAI	GACNNNNGTC	1 cut(s)	1230
BstPI	GGTNACC	1 cut(s)	1177
BstSCI	CCNGG	5 cut(s)	155, 456, 1724, 1914, 2083
BstSFI	CTRYAG	1 cut(s)	1143
BstSLI	GKGCMC	1 cut(s)	38
BstV1I	GCAGC	9 cut(s)	602, 1048, 1129, 1157, 1189, 1192, 1807, 2242, 2245
BstV2I	GAAGAC	1 cut(s)	2038
BstX2I	RGATCY	3 cut(s)	637, 1556, 1777
BstYI	RGATCY	3 cut(s)	637, 1556, 1777
BsuI	GTATCC	2 cut(s)	14, 442
BsuRI	GGCC	9 cut(s)	36, 293, 410, 471, 1106, 1364, 1397, 1680, 1757
BtgI	CCRYGG	1 cut(s)	288
BtsCI	GGATG	7 cut(s)	82, 1123, 1689, 1771, 1968, 1996, 2097
BtsI	GCAGTG	3 cut(s)	94, 783, 996
BtsIMutI	CAGTG	5 cut(s)	94, 480, 783, 996, 2293
BveI	ACCTGC	1 cut(s)	751
Cac8I	GCNNGC	7 cut(s)	660, 933, 1053, 1654, 1860, 2157, 2283
CaiI	CAGNNNCTG	1 cut(s)	1682
CfoI	GCGC	1 cut(s)	439
Cfr10I	RCCGGY	2 cut(s)	1106, 1790
Cfr13I	GGNCC	9 cut(s)	34, 35, 374, 470, 1345, 1679, 1701, 2081, 2161
Csp6I	GTAC	7 cut(s)	461, 740, 813, 859, 924, 1495, 2218
CviAII	CATG	7 cut(s)	289, 603, 634, 1463, 1486, 1942, 1987
CviQI	GTAC	7 cut(s)	461, 740, 813, 859, 924, 1495, 2218
DrdI	GACNNNNNNGTC	3 cut(s)	965, 1451, 1880
DseDI	GACNNNNNNGTC	3 cut(s)	965, 1451, 1880
EaeI	YGGCCR	3 cut(s)	291, 1104, 1755
Eam1104I	CTCTTC	2 cut(s)	543, 993
EarI	CTCTTC	2 cut(s)	543, 993
EciI	GGCGGA	1 cut(s)	1337
Ecl136II	GAGCTC	2 cut(s)	49, 583
Eco130I	CCWWGG	3 cut(s)	60, 288, 1172
Eco24I	GRGCYC	4 cut(s)	38, 51, 585, 624
Eco32I	GATATC	1 cut(s)	1966
Eco47I	GGWCC	5 cut(s)	374, 1345, 1701, 2081, 2161
Eco47III	AGCGCT	1 cut(s)	438
Eco53kI	GAGCTC	2 cut(s)	49, 583
Eco57I	CTGAAG	1 cut(s)	1415
Eco88I	CYCGRG	1 cut(s)	2165
Eco91I	GGTNACC	1 cut(s)	1177
EcoICRI	GAGCTC	2 cut(s)	49, 583
EcoO109I	RGGNCCY	3 cut(s)	35, 374, 1679
EcoO65I	GGTNACC	1 cut(s)	1177
EcoRI	GAATTC	1 cut(s)	2014
EcoRII	CCWGG	2 cut(s)	1724, 1914
EcoRV	GATATC	1 cut(s)	1966
EcoT14I	CCWWGG	3 cut(s)	60, 288, 1172
EcoT38I	GRGCYC	4 cut(s)	38, 51, 585, 624
ErhI	CCWWGG	3 cut(s)	60, 288, 1172
Esp3I	CGTCTC	2 cut(s)	12, 976
FaeI	CATG	7 cut(s)	292, 606, 637, 1466, 1489, 1945, 1990
FalI	AAGNNNNNCTT	8 cut(s)	44, 76, 827, 859, 848, 880, 948, 980
FaqI	GGGAC	3 cut(s)	347, 440, 1153
FatI	CATG	7 cut(s)	288, 602, 633, 1462, 1485, 1941, 1986
FauNDI	CATATG	1 cut(s)	394
FbaI	TGATCA	1 cut(s)	2001
FokI	GGATG	7 cut(s)	89, 1130, 1676, 1778, 1955, 2003, 2084
FriOI	GRGCYC	4 cut(s)	38, 51, 585, 624
FspBI	CTAG	2 cut(s)	224, 542
GlaI	GCGC	1 cut(s)	438
GsaI	CCCAGC	1 cut(s)	177
GsuI	CTGGAG	1 cut(s)	615
HaeII	RGCGCY	1 cut(s)	440
HaeIII	GGCC	9 cut(s)	36, 293, 410, 471, 1106, 1364, 1397, 1680, 1757
HapII	CCGG	7 cut(s)	156, 458, 702, 1107, 1791, 2084, 2140
HhaI	GCGC	1 cut(s)	439
Hin1I	GRCGYC	2 cut(s)	5, 381
Hin1II	CATG	7 cut(s)	292, 606, 637, 1466, 1489, 1945, 1990
Hin6I	GCGC	1 cut(s)	437
HinP1I	GCGC	1 cut(s)	437
HincII	GTYRAC	2 cut(s)	693, 2188
HindII	GTYRAC	2 cut(s)	693, 2188
HindIII	AAGCTT	2 cut(s)	1277, 1427
HinfI	GANTC	7 cut(s)	65, 607, 644, 689, 760, 1930, 2209
HpaII	CCGG	7 cut(s)	156, 458, 702, 1107, 1791, 2084, 2140
HphI	GGTGA	5 cut(s)	272, 1189, 1601, 1996, 2239
Hpy166II	GTNNAC	3 cut(s)	693, 1885, 2188
Hpy8I	GTNNAC	3 cut(s)	693, 1885, 2188
Hpy99I	CGWCG	1 cut(s)	1236
HpyAV	CCTTC	9 cut(s)	193, 499, 769, 879, 935, 1210, 1335, 1657, 1826
HpyCH4III	ACNGT	5 cut(s)	454, 1232, 1531, 1618, 2198
HpyCH4IV	ACGT	2 cut(s)	381, 1905
HpyCH4V	TGCA	7 cut(s)	103, 658, 931, 1145, 1195, 1546, 1823
HpyF10VI	GCNNNNNNNGC	7 cut(s)	596, 951, 1061, 1151, 1208, 2243, 2252
HpySE526I	ACGT	2 cut(s)	381, 1905
Hsp92I	GRCGYC	2 cut(s)	5, 381
Hsp92II	CATG	7 cut(s)	292, 606, 637, 1466, 1489, 1945, 1990
HspAI	GCGC	1 cut(s)	437
Ksp22I	TGATCA	1 cut(s)	2001
LmnI	GCTCC	4 cut(s)	596, 1438, 1468, 2040
Lsp1109I	GCAGC	9 cut(s)	602, 1048, 1129, 1157, 1189, 1192, 1807, 2242, 2245
LweI	GCATC	5 cut(s)	142, 661, 1252, 1981, 2142
MaeI	CTAG	2 cut(s)	224, 542
MaeII	ACGT	2 cut(s)	381, 1905
MaeIII	GTNAC	4 cut(s)	781, 839, 1177, 1684
MboII	GAAGA	7 cut(s)	107, 530, 980, 983, 1277, 1285, 2038
MflI	RGATCY	3 cut(s)	637, 1556, 1777
MhlI	GDGCHC	4 cut(s)	38, 51, 585, 624
MlsI	TGGCCA	2 cut(s)	293, 1757
MluCI	AATT	6 cut(s)	386, 1071, 1410, 1508, 1809, 2014
MluNI	TGGCCA	2 cut(s)	293, 1757
MlyI	GAGTC	3 cut(s)	698, 754, 2203
MmeI	TCCRAC	2 cut(s)	230, 1977
Mox20I	TGGCCA	2 cut(s)	293, 1757
MscI	TGGCCA	2 cut(s)	293, 1757
MseI	TTAA	9 cut(s)	414, 834, 1014, 1083, 1281, 1401, 1425, 1826, 2145
MslI	CAYNNNNRTG	3 cut(s)	1233, 1940, 1985
Msp20I	TGGCCA	2 cut(s)	293, 1757
MspA1I	CMGCKG	1 cut(s)	593
MspI	CCGG	7 cut(s)	156, 458, 702, 1107, 1791, 2084, 2140
MspR9I	CCNGG	5 cut(s)	157, 458, 1726, 1916, 2085
Mva1269I	GAATGC	2 cut(s)	752, 1674
MvaI	CCWGG	2 cut(s)	1726, 1916
MwoI	GCNNNNNNNGC	7 cut(s)	596, 951, 1061, 1151, 1208, 2243, 2252
NciI	CCSGG	3 cut(s)	157, 458, 2085
NcoI	CCATGG	1 cut(s)	288
NdeI	CATATG	1 cut(s)	394
NlaIII	CATG	7 cut(s)	292, 606, 637, 1466, 1489, 1945, 1990
NlaIV	GGNNCC	5 cut(s)	36, 37, 375, 1346, 1470
NmuCI	GTSAC	3 cut(s)	781, 839, 1177
NspI	RCATGY	1 cut(s)	1945
OliI	CACNNNNGTG	1 cut(s)	1233
PaeR7I	CTCGAG	1 cut(s)	2165
PciI	ACATGT	1 cut(s)	1941
PctI	GAATGC	2 cut(s)	752, 1674
PfeI	GAWTC	4 cut(s)	65, 607, 644, 1930
PflMI	CCANNNNNTGG	1 cut(s)	245
PfoI	TCCNGGA	1 cut(s)	1914
PleI	GAGTC	3 cut(s)	697, 754, 2203
PpsI	GAGTC	3 cut(s)	697, 754, 2203
PpuMI	RGGWCCY	1 cut(s)	374
PscI	ACATGT	1 cut(s)	1941
PshAI	GACNNNNGTC	1 cut(s)	1230
Psp124BI	GAGCTC	2 cut(s)	51, 585
Psp1406I	AACGTT	1 cut(s)	1905
Psp5II	RGGWCCY	1 cut(s)	374
Psp6I	CCWGG	2 cut(s)	1724, 1914
PspEI	GGTNACC	1 cut(s)	1177
PspFI	CCCAGC	1 cut(s)	173
PspGI	CCWGG	2 cut(s)	1724, 1914
PspN4I	GGNNCC	5 cut(s)	36, 37, 375, 1346, 1470
PspOMI	GGGCCC	1 cut(s)	34
PspPI	GGNCC	9 cut(s)	34, 35, 374, 470, 1345, 1679, 1701, 2081, 2161
PspPPI	RGGWCCY	1 cut(s)	374
PstI	CTGCAG	1 cut(s)	1147
PstNI	CAGNNNCTG	1 cut(s)	1682
PsuI	RGATCY	3 cut(s)	637, 1556, 1777
PvuII	CAGCTG	1 cut(s)	593
RsaI	GTAC	7 cut(s)	462, 741, 814, 860, 925, 1496, 2219
RsaNI	GTAC	7 cut(s)	461, 740, 813, 859, 924, 1495, 2218
RseI	CAYNNNNRTG	3 cut(s)	1233, 1940, 1985
SacI	GAGCTC	2 cut(s)	51, 585
SaqAI	TTAA	9 cut(s)	414, 834, 1014, 1083, 1281, 1401, 1425, 1826, 2145
Sau96I	GGNCC	9 cut(s)	34, 35, 374, 470, 1345, 1679, 1701, 2081, 2161
ScaI	AGTACT	1 cut(s)	1496
SchI	GAGTC	3 cut(s)	698, 754, 2203
ScrFI	CCNGG	5 cut(s)	157, 458, 1726, 1916, 2085
SduI	GDGCHC	4 cut(s)	38, 51, 585, 624
SfaNI	GCATC	5 cut(s)	142, 661, 1252, 1981, 2142
SfcI	CTRYAG	1 cut(s)	1143
Sfr274I	CTCGAG	1 cut(s)	2165
SinI	GGWCC	5 cut(s)	374, 1345, 1701, 2081, 2161
SlaI	CTCGAG	1 cut(s)	2165
SmiMI	CAYNNNNRTG	3 cut(s)	1233, 1940, 1985
SmlI	CTYRAG	5 cut(s)	50, 584, 1132, 1781, 2165
SmoI	CTYRAG	5 cut(s)	50, 584, 1132, 1781, 2165
Sse9I	AATT	6 cut(s)	386, 1071, 1410, 1508, 1809, 2014
SsiI	CCGC	6 cut(s)	216, 1208, 1348, 1600, 2159, 2272
SspMI	CTAG	2 cut(s)	224, 542
SstI	GAGCTC	2 cut(s)	51, 585
StyD4I	CCNGG	5 cut(s)	155, 456, 1724, 1914, 2083
StyI	CCWWGG	3 cut(s)	60, 288, 1172
TaaI	ACNGT	5 cut(s)	454, 1232, 1531, 1618, 2198
TaiI	ACGT	2 cut(s)	384, 1908
TaqI	TCGA	4 cut(s)	339, 1561, 1983, 2166
TasI	AATT	6 cut(s)	386, 1071, 1410, 1508, 1809, 2014
TatI	WGTACW	2 cut(s)	923, 1494
TauI	GCSGC	2 cut(s)	1211, 1602
TfiI	GAWTC	4 cut(s)	65, 607, 644, 1930
Tru1I	TTAA	9 cut(s)	414, 834, 1014, 1083, 1281, 1401, 1425, 1826, 2145
Tru9I	TTAA	9 cut(s)	414, 834, 1014, 1083, 1281, 1401, 1425, 1826, 2145
TscAI	CASTG	5 cut(s)	94, 480, 790, 1003, 2293
TseFI	GTSAC	3 cut(s)	781, 839, 1177
TseI	GCWGC	9 cut(s)	590, 1061, 1142, 1145, 1202, 1205, 1820, 2255, 2258
Tsp45I	GTSAC	3 cut(s)	781, 839, 1177
TspDTI	ATGAA	6 cut(s)	346, 591, 1050, 1451, 1676, 1782
TspRI	CASTG	5 cut(s)	94, 480, 790, 1003, 2293
Van91I	CCANNNNNTGG	1 cut(s)	245
VpaK11BI	GGWCC	5 cut(s)	374, 1345, 1701, 2081, 2161
XapI	RAATTY	2 cut(s)	386, 2014
XceI	RCATGY	1 cut(s)	1945
XcmI	CCANNNNNNNNNTGG	2 cut(s)	246, 631
XhoI	CTCGAG	1 cut(s)	2165
XspI	CTAG	2 cut(s)	224, 542
ZraI	GACGTC	1 cut(s)	382
ZrmI	AGTACT	1 cut(s)	1496

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.