Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0004023

Physical Location & Seq

Genomic Coordinates Reverse (-)

588141 .. 593214

Gene Structure 5074 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1005500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2268 bp

ATGGGCCCTAAGAGAGAGCTCAAATTTGCTCTTGAATCTTTCTGGGATGGGAAGAGCAGTGCCGAGGACTTGCAGAAGGTGTCTGCTGATCTCAGATCTTCCATCTGGAAGCAGATGACTGATGCCGGGATCAAGCACATTCCCAGCAACACCTTCTCATACTACGATCAGGTGCTCGACACCACCGCATTGCTCGGGGCTGTCCCGCCAAGATATGGGTGGAATGGCGGTGAGATAGGATTTGATACATACTTCTCCATGGCCAGAGGAAATGCCTCTGTCCCTGCTATGGAAATGACCAAGTGGTTTGATACCAACTACCATTTCATTGTCCCTGAGTTGGGCCCTGATGTGAACTTCTCTTATGCTTCTCACAAGGCTGTTGAGGAATACAAGGAGGCTAAGGCGCTTGGAGTGGATACTGTTCCAGTCCTCGTTGGCCCAGTGTCATACTTGTTGCTGTCCAAGCCAGCAAAGGGTGTTGAGAAGACCTTTTCTCTTCTCTCTCTTCTTCCCAAAATTCTTCCCGTCTACAAGGAAGTAGTATCTGAGCTTAAGGCAGCTGGAGCTTCATGGATTCAATTTGATGAGCCTACACTTGTTTTGGATCTTGAATCCGACAAGTTGCAAGCATTCACTGATGCCTACTCTGAACTGGAGTCAACTCTATCTGGCTTGAATGTTCTTATTGAGACCTACTTCGCTGATGTTCCTGCTGCGGCATTCAAGATCCTCACTAGCTTGAAGGGTGTCACCGCATATGGTTTTGATTTGGTCCGTGGTACCAAGACCCTGGATTTGATTAAGAGCGAGTTCCCCAAGGGAAAGTACCTCTTTGCCGGTTTGGTTGATGGAAGGAACATCTGGGCTAATGACCTTTCTGCCTCTCTAAGCACTTTGTTGACCCTTGAAGGCATTGTTGGAAAAGATAAACTTGTTGTCTCCACCTCCACCTCTCTTCTTCACACTGCTGTTGACCTTGTCAATGAGACCAAGCTCGACCAGGAAATTAAGTCATGGTTGGCTTTTGCTGCCCAGAAAATTGTTGAAGTTAATGCTTTGGCCAAGGCCTTGGCTGGTCACAAGGATGAGGCATTTTTCTCTGCTAATGCCGCTGCCTTGGCTTCAAGGAAGTCCTCCCCAAGGGTGACCAATGAGGCTGTCCAGAAGGCTGCTGCTGCTTTGAAGGATTCTGACCACCGTCGTGCTACCAATGTTAGTGCTAGACTTGATGCTCAACAGAAGAAGCTTAACCTTCCAATTCTCCCAACCACCACCATTGGATCGTTCCCTCAGACCATTGAACTCAGGAGGGTCCGTCGTGAGTACAAGGCCAAGAAGATTTCCGAGGAGGAATATGTCAAGGCCATCAAGGAAGAAATTAACAAGGTTGTTAAACTTCAGGAAGAACTTGACATTGATGTCCTTGTTCATGGAGAGCCTGAGAGGAACGATATGGTTGAGTACTTCGGAGAACAATTGTCTGGCTTTGCCTTTACTGCCAATGGGTGGGTGCAATCATATGGATCTCGATGTGTGAAGCCACCAATCATCTATGGTGATGTGAGCCGCCCCAAGCCTATGACTGTGTTCTGGTCATCTGCTGCTCAGAGCATGACTGCCCGCCCAATGAAGGGTATGCTTACGGGCCCCGTCACCATCCTGAACTGGTCTTTCGTCAGAAATGACCAGCCCAGATTCGAGACCACCTACCAGATTGCTTTGGCCATCAAGGATGAAGTGGAGGACCTTGAGAAGGCCGGTATCAGTGTAATTCAGATCGATGAGGCTGCTTTGAGAGAGGGATTGCCTCTTAGGAAATCTGAACATGCTTTCTACTTGGATTGGGCTGTGCACTCCTTCAGGATCACCAACAGTGGTGTACAGGACACTACCCAGATTCACACTCACATGTGCTACTCCAACTTCAATGACATCATCCACTCAATCATCGACATGGACGCTGATGTGATCACCATTGAGAACTCTCGTTCTGATGAGAAGCTTCTGTCAGTTTTCCGTGAGGGAGTCAAGTATGGTGCTGGAATTGGCCCCGGTGTGTACGACATCCACTCTCCAAGAATACCATCAACCGACGAGATTGCTGACAGGATCAACAAGATGCTTGCGGTGCTGGAGACCAACATCTTGTGGGTGAACCCCGACTGTGGGCTCAAGACCCGCAAGTACACAGAAGTGAAGCCGGCCCTCAGCAACATGGTTGCTGCCGCCAAGCTCCTCCGCACCCAGCTCGCCAGTGCCAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000096 GO:0000097 GO:0003674 GO:0003824 GO:0003871 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005777 GO:0005829 GO:0005886 GO:0005911 GO:0006082 GO:0006520 GO:0006555 GO:0006790 GO:0006807 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0008168 GO:0008172 GO:0008270 GO:0008652 GO:0008705 GO:0009058 GO:0009066 GO:0009067 GO:0009086 GO:0009506 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009651 GO:0009941 GO:0009987 GO:0010035 GO:0010038 GO:0010043 GO:0016020 GO:0016053 GO:0016740 GO:0016741 GO:0019752 GO:0030054 GO:0031090 GO:0031967 GO:0031975 GO:0032259 GO:0042084 GO:0042085 GO:0042221 GO:0042579 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044272 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046686 GO:0046872 GO:0046914 GO:0048046 GO:0050667 GO:0050896 GO:0055044 GO:0071704 GO:0071944 GO:0098588 GO:0098805 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00270 ko00450 ko01100 ko01110 ko01230 map00270 map00450 map01100 map01110 map01230

Pfam Domains

Protein Families

Meth_synt_1 Meth_synt_2

Protein Analysis

756

Amino Acids

83.56

Weight (kDa)

5.93

Isoelectric Point (pI)

33.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Meth_synt_1	PF08267	1 - 305	4.3e-111	Cobalamin-independent synthase, N-terminal domain
Meth_synt_2	PF01717	422 - 745	1.2e-159	Cobalamin-independent synthase, Catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0004023)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G03780 AT3G03780 AT3G03780 AT5G17920 AT5G17920
fragaria_vesca	FvH4_1g28390 FvH4_1g28390 FvH4_1g28390 FvH4_1g28390 FvH4_2g41260 FvH4_2g41260 FvH4_2g41260 FvH4_2g41260 FvH4_2g41260 FvH4_2g41260
malus_domestica	MD12G1008100.v1.1 MD14G1005500.v1.1
prunus_persica	Prupe.7G009200_v2.0.a1 Prupe.7G009200_v2.0.a1 Prupe.7G009200_v2.0.a1
pyrus_communis	pycom12g00610 pycom14g00410
rosa_chinensis	RchiOBHm_Chr3g0496511
rosa_laevigata	RLG00000022522
rosa_multiflora	Rmu_sc0000620.1_g000008
rosa_roxburghii	Rroxscaffold_6G00389060
rosa_rugosa	Rorug03G0283400 Rorug03G0283500
rosa_samantha	Rh3AG329600 Rh3BG365100 Rh3CG362500 Rh3DG365600
rosa_wichuraiana	Rw3G028830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1421
Acc65I	GGTACC	1 cut(s)	782
AccB1I	GGYRCC	1 cut(s)	782
AccB7I	CCANNNNNTGG	2 cut(s)	215, 1509
AccI	GTMKAC	1 cut(s)	531
AclWI	GGATC	7 cut(s)	137, 615, 724, 1291, 1534, 1874, 2120
AcoI	YGGCCR	3 cut(s)	261, 1062, 1725
AcsI	RAATTY	2 cut(s)	23, 519
AcuI	CTGAAG	2 cut(s)	1385, 1846
AfaI	GTAC	7 cut(s)	784, 830, 1328, 1466, 1884, 2063, 2189
AflII	CTTAAG	1 cut(s)	554
AflIII	ACRYGT	1 cut(s)	1911
AjnI	CCWGG	2 cut(s)	792, 1002
AjuI	GAANNNNNNNTTGG	4 cut(s)	308, 340, 903, 935
AleI	CACNNNNGTG	2 cut(s)	1203, 2195
Alw21I	GWGCWC	3 cut(s)	21, 177, 1857
Alw26I	GTCTC	5 cut(s)	686, 946, 983, 1697, 2132
Alw44I	GTGCAC	1 cut(s)	1853
AlwI	GGATC	7 cut(s)	137, 615, 724, 1291, 1534, 1874, 2120
Ama87I	CYCGRG	1 cut(s)	194
ApaI	GGGCCC	3 cut(s)	8, 347, 1652
ApaLI	GTGCAC	1 cut(s)	1853
ApoI	RAATTY	2 cut(s)	23, 519
ArsI	GACNNNNNNTTYG	2 cut(s)	781, 813
Asp718I	GGTACC	1 cut(s)	782
AspLEI	GCGC	1 cut(s)	409
AsuC2I	CCSGG	2 cut(s)	127, 2055
AsuHPI	GGTGA	8 cut(s)	242, 745, 1159, 1571, 1648, 1861, 1966, 2167
AvaI	CYCGRG	1 cut(s)	194
AvaII	GGWCC	3 cut(s)	775, 1315, 1747
BaeGI	GKGCMC	4 cut(s)	8, 347, 1652, 1857
BalI	TGGCCA	3 cut(s)	263, 1064, 1727
BanI	GGYRCC	1 cut(s)	782
BanII	GRGCYC	5 cut(s)	8, 21, 347, 1652, 2175
BbsI	GAAGAC	1 cut(s)	494
Bbv12I	GWGCWC	3 cut(s)	21, 177, 1857
BbvCI	CCTCAGC	1 cut(s)	2210
BccI	CCATC	7 cut(s)	41, 110, 845, 1376, 1667, 1736, 2095
BcgI	CGANNNNNNTGC	6 cut(s)	1772, 1806, 2084, 2118, 2233, 2267
BciT130I	CCWGG	2 cut(s)	794, 1004
BciVI	GTATCC	1 cut(s)	412
BclI	TGATCA	1 cut(s)	1971
BcnI	CCSGG	2 cut(s)	127, 2055
BcoDI	GTCTC	5 cut(s)	686, 946, 983, 1697, 2132
BfaI	CTAG	2 cut(s)	738, 1224
BfoI	RGCGCY	1 cut(s)	410
BfrI	CTTAAG	1 cut(s)	554
BfuI	GTATCC	1 cut(s)	412
BglII	AGATCT	1 cut(s)	95
BmcAI	AGTACT	1 cut(s)	1466
Bme1390I	CCNGG	4 cut(s)	127, 794, 1004, 2055
Bme18I	GGWCC	3 cut(s)	775, 1315, 1747
BmeT110I	CYCGRG	1 cut(s)	194
BmiI	GGNNCC	7 cut(s)	6, 345, 784, 1316, 1650, 1651, 2053
BmrFI	CCNGG	4 cut(s)	127, 794, 1004, 2055
BmrI	ACTGGG	1 cut(s)	437
BmsI	GCATC	4 cut(s)	112, 631, 1222, 2112
BmuI	ACTGGG	1 cut(s)	437
BoxI	GACNNNNGTC	1 cut(s)	1200
BpiI	GAAGAC	1 cut(s)	494
BpmI	CTGGAG	3 cut(s)	585, 677, 2156
Bpu10I	CCTNAGC	2 cut(s)	402, 2210
BpuEI	CTTGAG	2 cut(s)	1772, 2159
BpuMI	CCSGG	2 cut(s)	127, 2055
Bsa29I	ATCGAT	1 cut(s)	1782
BsaI	GGTCTC	4 cut(s)	686, 983, 1697, 2132
BsaXI	ACNNNNNCTCC	2 cut(s)	1303, 1333
Bse118I	RCCGGY	3 cut(s)	839, 1760, 2203
Bse1I	ACTGG	5 cut(s)	428, 443, 660, 1673, 2256
Bse3DI	GCAATG	1 cut(s)	188
BseBI	CCWGG	2 cut(s)	794, 1004
BseCI	ATCGAT	1 cut(s)	1782
BseGI	GGATG	6 cut(s)	52, 1093, 1659, 1741, 1938, 2067
BseMI	GCAATG	1 cut(s)	188
BseMII	CTCAG	8 cut(s)	106, 327, 540, 1307, 1321, 1434, 1622, 2224
BseNI	ACTGG	5 cut(s)	428, 443, 660, 1673, 2256
BseRI	GAGGAG	2 cut(s)	1364, 2228
BseSI	GKGCMC	4 cut(s)	8, 347, 1652, 1857
BseYI	CCCAGC	2 cut(s)	143, 2247
BshNI	GGYRCC	1 cut(s)	782
BshVI	ATCGAT	1 cut(s)	1782
BsiHKAI	GWGCWC	3 cut(s)	21, 177, 1857
BsiHKCI	CYCGRG	1 cut(s)	194
BsiSI	CCGG	5 cut(s)	126, 840, 1761, 2055, 2204
BslFI	GGGAC	3 cut(s)	188, 266, 317
BsmAI	GTCTC	5 cut(s)	686, 946, 983, 1697, 2132
BsmFI	GGGAC	3 cut(s)	188, 266, 317
BsmI	GAATGC	2 cut(s)	632, 722
Bso31I	GGTCTC	4 cut(s)	686, 983, 1697, 2132
BsoBI	CYCGRG	1 cut(s)	194
Bsp120I	GGGCCC	3 cut(s)	4, 343, 1648
Bsp1286I	GDGCHC	7 cut(s)	8, 21, 177, 347, 1652, 1857, 2175
Bsp1407I	TGTACA	1 cut(s)	1882
Bsp19I	CCATGG	1 cut(s)	258
BspCNI	CTCAG	8 cut(s)	105, 328, 541, 1306, 1320, 1435, 1621, 2223
BspDI	ATCGAT	1 cut(s)	1782
BspLI	GGNNCC	7 cut(s)	6, 345, 784, 1316, 1650, 1651, 2053
BspPI	GGATC	7 cut(s)	137, 615, 724, 1291, 1534, 1874, 2120
BspQI	GCTCTTC	1 cut(s)	47
BspT107I	GGYRCC	1 cut(s)	782
BspTI	CTTAAG	1 cut(s)	554
BspTNI	GGTCTC	4 cut(s)	686, 983, 1697, 2132
BsrDI	GCAATG	1 cut(s)	188
BsrFI	RCCGGY	3 cut(s)	839, 1760, 2203
BsrGI	TGTACA	1 cut(s)	1882
BsrI	ACTGG	5 cut(s)	428, 443, 660, 1673, 2256
BssAI	RCCGGY	3 cut(s)	839, 1760, 2203
BssT1I	CCWWGG	6 cut(s)	258, 819, 1065, 1071, 1119, 1142
Bst2UI	CCWGG	2 cut(s)	794, 1004
Bst4CI	ACNGT	5 cut(s)	424, 1202, 1588, 1877, 2168
Bst6I	CTCTTC	4 cut(s)	47, 504, 513, 963
BstAFI	CTTAAG	1 cut(s)	554
BstAUI	TGTACA	1 cut(s)	1882
BstC8I	GCNNGC	6 cut(s)	471, 630, 1624, 2127, 2205, 2253
BstDSI	CCRYGG	2 cut(s)	258, 778
BstEII	GGTNACC	1 cut(s)	1147
BstENI	CCTNNNNNAGG	2 cut(s)	1141, 1754
BstF5I	GGATG	6 cut(s)	52, 1093, 1659, 1741, 1938, 2067
BstH2I	RGCGCY	1 cut(s)	410
BstHHI	GCGC	1 cut(s)	409
BstMAI	GTCTC	5 cut(s)	686, 946, 983, 1697, 2132
BstMWI	GCNNNNNNNGC	8 cut(s)	466, 566, 1031, 1112, 1121, 1178, 1499, 2131
BstNI	CCWGG	2 cut(s)	794, 1004
BstNSI	RCATGY	2 cut(s)	1832, 1915
BstPAI	GACNNNNGTC	1 cut(s)	1200
BstPI	GGTNACC	1 cut(s)	1147
BstSCI	CCNGG	4 cut(s)	125, 792, 1002, 2053
BstSLI	GKGCMC	4 cut(s)	8, 347, 1652, 1857
BstV2I	GAAGAC	1 cut(s)	494
BstX2I	RGATCY	4 cut(s)	95, 607, 729, 1526
BstXI	CCANNNNNNTGG	2 cut(s)	793, 1072
BstYI	RGATCY	4 cut(s)	95, 607, 729, 1526
Bsu15I	ATCGAT	1 cut(s)	1782
BsuI	GTATCC	1 cut(s)	412
BsuTUI	ATCGAT	1 cut(s)	1782
BtgI	CCRYGG	2 cut(s)	258, 778
BtsCI	GGATG	6 cut(s)	52, 1093, 1659, 1741, 1938, 2067
BtsI	GCAGTG	2 cut(s)	64, 966
BtsIMutI	CAGTG	7 cut(s)	64, 450, 636, 966, 1774, 1882, 2263
Cac8I	GCNNGC	6 cut(s)	471, 630, 1624, 2127, 2205, 2253
CfoI	GCGC	1 cut(s)	409
Cfr10I	RCCGGY	3 cut(s)	839, 1760, 2203
ClaI	ATCGAT	1 cut(s)	1782
CseI	GACGC	1 cut(s)	1970
Csp6I	GTAC	7 cut(s)	783, 829, 1327, 1465, 1883, 2062, 2188
CspCI	CAANNNNNGTGG	2 cut(s)	1261, 1296
CviAII	CATG	9 cut(s)	259, 573, 1017, 1433, 1615, 1829, 1912, 1957, 2218
CviQI	GTAC	7 cut(s)	783, 829, 1327, 1465, 1883, 2062, 2188
DrdI	GACNNNNNNGTC	1 cut(s)	1421
DseDI	GACNNNNNNGTC	1 cut(s)	1421
EaeI	YGGCCR	3 cut(s)	261, 1062, 1725
Eam1104I	CTCTTC	4 cut(s)	47, 504, 513, 963
EarI	CTCTTC	4 cut(s)	47, 504, 513, 963
Ecl136II	GAGCTC	1 cut(s)	19
Eco130I	CCWWGG	6 cut(s)	258, 819, 1065, 1071, 1119, 1142
Eco147I	AGGCCT	1 cut(s)	1070
Eco24I	GRGCYC	5 cut(s)	8, 21, 347, 1652, 2175
Eco31I	GGTCTC	4 cut(s)	686, 983, 1697, 2132
Eco47I	GGWCC	3 cut(s)	775, 1315, 1747
Eco53kI	GAGCTC	1 cut(s)	19
Eco57I	CTGAAG	2 cut(s)	1385, 1846
Eco88I	CYCGRG	1 cut(s)	194
Eco91I	GGTNACC	1 cut(s)	1147
EcoICRI	GAGCTC	1 cut(s)	19
EcoNI	CCTNNNNNAGG	2 cut(s)	1141, 1754
EcoO109I	RGGNCCY	4 cut(s)	5, 344, 1649, 1747
EcoO65I	GGTNACC	1 cut(s)	1147
EcoRII	CCWGG	2 cut(s)	792, 1002
EcoT14I	CCWWGG	6 cut(s)	258, 819, 1065, 1071, 1119, 1142
EcoT38I	GRGCYC	5 cut(s)	8, 21, 347, 1652, 2175
ErhI	CCWWGG	6 cut(s)	258, 819, 1065, 1071, 1119, 1142
FaeI	CATG	9 cut(s)	262, 576, 1020, 1436, 1618, 1832, 1915, 1960, 2221
FalI	AAGNNNNNCTT	4 cut(s)	818, 850, 918, 950
FaqI	GGGAC	3 cut(s)	188, 266, 317
FatI	CATG	9 cut(s)	258, 572, 1016, 1432, 1614, 1828, 1911, 1956, 2217
FauI	CCCGC	3 cut(s)	213, 1631, 2189
FauNDI	CATATG	2 cut(s)	760, 1522
FbaI	TGATCA	1 cut(s)	1971
FblI	GTMKAC	1 cut(s)	531
FokI	GGATG	6 cut(s)	59, 1100, 1646, 1748, 1925, 2054
FriOI	GRGCYC	5 cut(s)	8, 21, 347, 1652, 2175
FspBI	CTAG	2 cut(s)	738, 1224
GlaI	GCGC	1 cut(s)	408
GsaI	CCCAGC	2 cut(s)	147, 2251
GsuI	CTGGAG	3 cut(s)	585, 677, 2156
HaeII	RGCGCY	1 cut(s)	410
HapII	CCGG	5 cut(s)	126, 840, 1761, 2055, 2204
HgaI	GACGC	1 cut(s)	1970
HhaI	GCGC	1 cut(s)	409
Hin1II	CATG	9 cut(s)	262, 576, 1020, 1436, 1618, 1832, 1915, 1960, 2221
Hin6I	GCGC	1 cut(s)	407
HinP1I	GCGC	1 cut(s)	407
HincII	GTYRAC	3 cut(s)	663, 903, 976
HindII	GTYRAC	3 cut(s)	663, 903, 976
HindIII	AAGCTT	2 cut(s)	1247, 2003
HinfI	GANTC	8 cut(s)	35, 577, 614, 659, 1190, 1698, 1900, 2028
HpaII	CCGG	5 cut(s)	126, 840, 1761, 2055, 2204
HphI	GGTGA	8 cut(s)	242, 745, 1159, 1571, 1648, 1861, 1966, 2167
Hpy99I	CGWCG	3 cut(s)	1206, 1323, 2099
HpyCH4III	ACNGT	5 cut(s)	424, 1202, 1588, 1877, 2168
HpyCH4V	TGCA	4 cut(s)	73, 628, 1516, 1855
HpyF10VI	GCNNNNNNNGC	8 cut(s)	466, 566, 1031, 1112, 1121, 1178, 1499, 2131
Hsp92II	CATG	9 cut(s)	262, 576, 1020, 1436, 1618, 1832, 1915, 1960, 2221
HspAI	GCGC	1 cut(s)	407
KpnI	GGTACC	1 cut(s)	786
KroI	GCCGGC	1 cut(s)	2203
KroNI	GCCGGC	1 cut(s)	2205
Ksp22I	TGATCA	1 cut(s)	1971
LguI	GCTCTTC	1 cut(s)	47
LmnI	GCTCC	2 cut(s)	566, 2241
LweI	GCATC	4 cut(s)	112, 631, 1222, 2112
MaeI	CTAG	2 cut(s)	738, 1224
MaeIII	GTNAC	4 cut(s)	751, 1079, 1147, 1654
MfeI	CAATTG	1 cut(s)	1478
MflI	RGATCY	4 cut(s)	95, 607, 729, 1526
MhlI	GDGCHC	7 cut(s)	8, 21, 177, 347, 1652, 1857, 2175
MlsI	TGGCCA	3 cut(s)	263, 1064, 1727
MluNI	TGGCCA	3 cut(s)	263, 1064, 1727
MlyI	GAGTC	2 cut(s)	668, 2037
MmeI	TCCRAC	3 cut(s)	642, 901, 1947
Mox20I	TGGCCA	3 cut(s)	263, 1064, 1727
MroNI	GCCGGC	1 cut(s)	2203
MscI	TGGCCA	3 cut(s)	263, 1064, 1727
MseI	TTAA	7 cut(s)	555, 804, 1011, 1053, 1251, 1383, 1395
MslI	CAYNNNNRTG	5 cut(s)	1086, 1203, 1910, 1955, 2195
Msp20I	TGGCCA	3 cut(s)	263, 1064, 1727
MspA1I	CMGCKG	2 cut(s)	563, 1115
MspCI	CTTAAG	1 cut(s)	554
MspI	CCGG	5 cut(s)	126, 840, 1761, 2055, 2204
MspR9I	CCNGG	4 cut(s)	127, 794, 1004, 2055
MunI	CAATTG	1 cut(s)	1478
Mva1269I	GAATGC	2 cut(s)	632, 722
MvaI	CCWGG	2 cut(s)	794, 1004
MwoI	GCNNNNNNNGC	8 cut(s)	466, 566, 1031, 1112, 1121, 1178, 1499, 2131
NaeI	GCCGGC	1 cut(s)	2205
NciI	CCSGG	2 cut(s)	127, 2055
NcoI	CCATGG	1 cut(s)	258
NdeI	CATATG	2 cut(s)	760, 1522
NgoMIV	GCCGGC	1 cut(s)	2203
NlaIII	CATG	9 cut(s)	262, 576, 1020, 1436, 1618, 1832, 1915, 1960, 2221
NlaIV	GGNNCC	7 cut(s)	6, 345, 784, 1316, 1650, 1651, 2053
NmeAIII	GCCGAG	1 cut(s)	88
NmuCI	GTSAC	4 cut(s)	751, 1079, 1147, 1654
NspI	RCATGY	2 cut(s)	1832, 1915
OliI	CACNNNNGTG	2 cut(s)	1203, 2195
PceI	AGGCCT	1 cut(s)	1070
PciI	ACATGT	1 cut(s)	1911
PciSI	GCTCTTC	1 cut(s)	47
PctI	GAATGC	2 cut(s)	632, 722
PdiI	GCCGGC	1 cut(s)	2205
PfeI	GAWTC	6 cut(s)	35, 577, 614, 1190, 1698, 1900
PflFI	GACNNNGTC	1 cut(s)	980
PflMI	CCANNNNNTGG	2 cut(s)	215, 1509
PleI	GAGTC	2 cut(s)	667, 2036
PpsI	GAGTC	2 cut(s)	667, 2036
PpuMI	RGGWCCY	1 cut(s)	1747
PscI	ACATGT	1 cut(s)	1911
PshAI	GACNNNNGTC	1 cut(s)	1200
Psp124BI	GAGCTC	1 cut(s)	21
Psp5II	RGGWCCY	1 cut(s)	1747
Psp6I	CCWGG	2 cut(s)	792, 1002
PspEI	GGTNACC	1 cut(s)	1147
PspFI	CCCAGC	2 cut(s)	143, 2247
PspGI	CCWGG	2 cut(s)	792, 1002
PspN4I	GGNNCC	7 cut(s)	6, 345, 784, 1316, 1650, 1651, 2053
PspOMI	GGGCCC	3 cut(s)	4, 343, 1648
PspPPI	RGGWCCY	1 cut(s)	1747
PsuI	RGATCY	4 cut(s)	95, 607, 729, 1526
PsyI	GACNNNGTC	1 cut(s)	980
PvuII	CAGCTG	1 cut(s)	563
RsaI	GTAC	7 cut(s)	784, 830, 1328, 1466, 1884, 2063, 2189
RsaNI	GTAC	7 cut(s)	783, 829, 1327, 1465, 1883, 2062, 2188
RseI	CAYNNNNRTG	5 cut(s)	1086, 1203, 1910, 1955, 2195
SacI	GAGCTC	1 cut(s)	21
SapI	GCTCTTC	1 cut(s)	47
SaqAI	TTAA	7 cut(s)	555, 804, 1011, 1053, 1251, 1383, 1395
ScaI	AGTACT	1 cut(s)	1466
SchI	GAGTC	2 cut(s)	668, 2037
ScrFI	CCNGG	4 cut(s)	127, 794, 1004, 2055
SduI	GDGCHC	7 cut(s)	8, 21, 177, 347, 1652, 1857, 2175
SfaNI	GCATC	4 cut(s)	112, 631, 1222, 2112
SinI	GGWCC	3 cut(s)	775, 1315, 1747
SmiMI	CAYNNNNRTG	5 cut(s)	1086, 1203, 1910, 1955, 2195
SmlI	CTYRAG	3 cut(s)	554, 1751, 2174
SmoI	CTYRAG	3 cut(s)	554, 1751, 2174
SseBI	AGGCCT	1 cut(s)	1070
SspMI	CTAG	2 cut(s)	738, 1224
SstI	GAGCTC	1 cut(s)	21
StuI	AGGCCT	1 cut(s)	1070
StyD4I	CCNGG	4 cut(s)	125, 792, 1002, 2053
StyI	CCWWGG	6 cut(s)	258, 819, 1065, 1071, 1119, 1142
TaaI	ACNGT	5 cut(s)	424, 1202, 1588, 1877, 2168
TaqI	TCGA	6 cut(s)	177, 999, 1531, 1701, 1782, 1953
TatI	WGTACW	4 cut(s)	1326, 1464, 1882, 2187
TauI	GCSGC	4 cut(s)	722, 1115, 1572, 2231
TfiI	GAWTC	6 cut(s)	35, 577, 614, 1190, 1698, 1900
Tru1I	TTAA	7 cut(s)	555, 804, 1011, 1053, 1251, 1383, 1395
Tru9I	TTAA	7 cut(s)	555, 804, 1011, 1053, 1251, 1383, 1395
TscAI	CASTG	7 cut(s)	64, 450, 643, 973, 1774, 1882, 2263
TseFI	GTSAC	4 cut(s)	751, 1079, 1147, 1654
Tsp45I	GTSAC	4 cut(s)	751, 1079, 1147, 1654
TspDTI	ATGAA	5 cut(s)	316, 561, 1421, 1646, 1752
TspGWI	ACGGA	3 cut(s)	767, 1307, 2009
TspRI	CASTG	7 cut(s)	64, 450, 643, 973, 1774, 1882, 2263
Tth111I	GACNNNGTC	1 cut(s)	980
Van91I	CCANNNNNTGG	2 cut(s)	215, 1509
Vha464I	CTTAAG	1 cut(s)	554
VneI	GTGCAC	1 cut(s)	1853
VpaK11BI	GGWCC	3 cut(s)	775, 1315, 1747
XagI	CCTNNNNNAGG	2 cut(s)	1141, 1754
XapI	RAATTY	2 cut(s)	23, 519
XceI	RCATGY	2 cut(s)	1832, 1915
XcmI	CCANNNNNNNNNTGG	1 cut(s)	216
XmiI	GTMKAC	1 cut(s)	531
XspI	CTAG	2 cut(s)	738, 1224
ZrmI	AGTACT	1 cut(s)	1466

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.