Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0004023

Physical Location & Seq

Genomic Coordinates Reverse (-)

2716549 .. 2721194

Gene Structure 4646 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000022522

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2361 bp

ATGTCAGATCTAGCAGATGTCATGAGCACTCAAGTCCTACCCCTCTCTACCTCCGAAAGAGAAATGGCGTCTCACATTGTTGGATATCCTCGTATGGGCCCCAAGAGAGAGCTCAAGTTTGCCTTGGAATCTTTCTGGGATGGCAAGAGCAGTGCCGAAGATTTGCAAAAGGTGTCTGCTGATCTCAGGTCTTCCATCTGGAAGCAGATGGCTGATGCCGGGATCATGCACATTCCCAGCAACACTTTCTCTCACTACGACCAGGTGCTTGACACCACTGCAATGCTTGGGGCAGTTCCACCAAGATATGGTTGGAATGGTGGTGAGATTGGATTTGACACTTATTTCTCCATGGCCAGAGGAAATGCCTCAGTTCCTGCTATGGAAATGACCAAGTGGTTTGATACCAACTACCATTTCATTGTCCCTGAGTTGGGTCCTGACGTCAAATTCTCATATGCTTCTCACAAGGCTGTTGAGGAATACAAGGAGGCTAAAGCGCTTGGAGTGGATACAATCCCAGTCCTTGTTGGTCCAGTGACCTATTTGTTGCTGTCCAAACCAGCAAAGGGTGTTGAAAAGACATTTTCGCTTCTATCTCTTCTAGGAAAAATCATTCCTATTTACAAGGAAGTTATATCTGAGCTTAAGGCAGCTGGAGCTTCATGGATTCAGTTTGATGAGCCCACACTTGTTATGGATCTTGATTCTCACAAGTTGCAAGCATTCACTGATGCTTACTCTCAGCTAGAATCAACTCTGTCCGGCTTGAATGTTCTTATTGAGACCTACTTTGCAGATGTACCTGCGGAGGCATACAAGACTCTCACTTCCTTGAAGGGAGTCACTGCCTATGGTTTTGATTTAGTTCGTGGTTCTAAGACCCTTGAGTTAATTAAGAGTGACTTCCCTAAAGGAAAGTGCCTCTTTGCTGGATTGGTTGATGGAAGGAACATTTGGGCTAATGATCTCTCAGCTTCCCTAAGTACATTGCAGGCCCTTGAAGGCATTGTTGGCAAAGACCACCTTGTTGTCTCTACCTCCTGCTCTTGTCTTCACACTGCTGTTGATCTTGTTAATGAGACTAAGCTGGACAATGAAATCAAATCATGGCTCGCCTTTGCTGCACAGAAAATTCTTGAAGTTAACGCTTTGGCAAAGGCATTGGCTGGTCACAAGGATGAGTCATTTTTCTCAAGCAATGCTGCAGCTCTGGCTTCAAGGAAATCGTCCCCAAGGGTGACCAATGAGGCTGTTCAAAAGGCTGCTGCTGCTTTGAAGGGTTCTGACCACCGTCGTGCTACTAATGTAAGTGCCAGACTTGATACTCAGCAGAAGAAGCTTAATCTTCCAATTCTCCCAACCACCACCATTGGATCATTCCCTCAGACCATTGAACTGAGAAGGGTTCGCCGTGAATACAAGGCCAAAAAGATCTCTGAGGAGGAGTATGTCAAGGCCATTAAGGAGGAAATTAGCAAAGTTGTCAAGCTTCAGGAAGAGCTTGACATTGATGTCCTTGTTCATGGGGAGCCTGAGAGGAACGACATGGTTGAGTACTTTGGGGAGCAATTATCAGGGTTTGCCTTTACTGTCAATGGGTGGGTGCAATCTTATGGATCTCGATGTGTGAAGCCACCAATCATCTATGGTGATGTGAGCCGCCCAAAGCCAATGACTGTGTTCTGGTCATCTGCTGCTCAGAGCATGACTGCCCGCCCAATGAAGGGAATGCTTACAGGCCCTGTTACCATCCTCAACTGGTCCTTTGTTAGAGTTGACCAGCCCAGGTCTGAGACTACCTACCAGATTGCTTTGGCCATCAAGGATGAAGTTGAGGATCTCGAGAAAGCTGGTATCAATGTTATTCAAATTGATGAGGCTGCGTTGAGAGAAGGGTTGCCTCTCAGGAAGTCTGAGCAAGCTCACTACTTGGATTGGGCCGTGCATTCATTCAGGATTACCAACGTTGGTGTCAAGGACACTACTCAGATTCACACTCACATGTGCTATTCCAACTTCAACGACATCATCCACTCCATCATCGACATGGATGCTGATGTGATCACCATTGAGAATTCTCGCTCTGATGAGAAGCTCCTGTCGGTCTTCCGCGAGGGAGTGAAGTATGGTGCTGGTATTGGTCCTGGTGTTTATGACATCCACTCTCCAAGAATACCATCAACTGAGGAGATTGCTGACCGGATTAACAAGATGCTTGCAGTCCTTGAGACAAATATCTTGTGGGTCAACCCAGACTGTGGGCTTAAGACTCGCAAGTACGCTGAGGTGAAGCCAGCACTCAGTGCTATGGTTGCTGCTGCCAAGCAACTCCGCACTCAGCTTGCCAGTGCTAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000096 GO:0000097 GO:0003674 GO:0003824 GO:0003871 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005777 GO:0005829 GO:0005886 GO:0005911 GO:0006082 GO:0006520 GO:0006555 GO:0006790 GO:0006807 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0008168 GO:0008172 GO:0008270 GO:0008652 GO:0008705 GO:0009058 GO:0009066 GO:0009067 GO:0009086 GO:0009506 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009651 GO:0009941 GO:0009987 GO:0010035 GO:0010038 GO:0010043 GO:0016020 GO:0016053 GO:0016740 GO:0016741 GO:0019752 GO:0030054 GO:0031090 GO:0031967 GO:0031975 GO:0032259 GO:0042084 GO:0042085 GO:0042221 GO:0042579 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044272 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046686 GO:0046872 GO:0046914 GO:0048046 GO:0050667 GO:0050896 GO:0055044 GO:0071704 GO:0071944 GO:0098588 GO:0098805 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00270 ko00450 ko01100 ko01110 ko01230 map00270 map00450 map01100 map01110 map01230

Pfam Domains

Protein Families

Meth_synt_1 Meth_synt_2

Protein Analysis

787

Amino Acids

86.87

Weight (kDa)

6.08

Isoelectric Point (pI)

37.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Meth_synt_1	PF08267	24 - 336	9.7e-117	Cobalamin-independent synthase, N-terminal domain
Meth_synt_2	PF01717	453 - 776	1.3e-158	Cobalamin-independent synthase, Catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0004023)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G03780 AT3G03780 AT3G03780 AT5G17920 AT5G17920
fragaria_vesca	FvH4_1g28390 FvH4_1g28390 FvH4_1g28390 FvH4_1g28390 FvH4_2g41260 FvH4_2g41260 FvH4_2g41260 FvH4_2g41260 FvH4_2g41260 FvH4_2g41260
malus_domestica	MD12G1008100.v1.1 MD14G1005500.v1.1
prunus_persica	Prupe.7G009200_v2.0.a1 Prupe.7G009200_v2.0.a1 Prupe.7G009200_v2.0.a1
pyrus_communis	pycom12g00610 pycom14g00410
rosa_chinensis	RchiOBHm_Chr3g0496511
rosa_laevigata	RLG00000022522
rosa_multiflora	Rmu_sc0000620.1_g000008
rosa_roxburghii	Rroxscaffold_6G00389060
rosa_rugosa	Rorug03G0283400 Rorug03G0283500
rosa_samantha	Rh3AG329600 Rh3BG365100 Rh3CG362500 Rh3DG365600
rosa_wichuraiana	Rw3G028830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1514
AatII	GACGTC	1 cut(s)	447
Acc36I	ACCTGC	1 cut(s)	814
AccB7I	CCANNNNNTGG	2 cut(s)	308, 2261
AccII	CGCG	1 cut(s)	2115
AciI	CCGC	5 cut(s)	809, 1663, 1717, 2113, 2335
AclI	AACGTT	1 cut(s)	1968
AclWI	GGATC	5 cut(s)	230, 708, 1384, 1627, 1848
AcoI	YGGCCR	2 cut(s)	354, 1818
AcsI	RAATTY	3 cut(s)	449, 1134, 2077
AcuI	CTGAAG	1 cut(s)	1478
AcyI	GRCGYC	2 cut(s)	68, 444
AdeI	CACNNNGTG	1 cut(s)	2306
AfaI	GTAC	4 cut(s)	804, 988, 1559, 2282
AfeI	AGCGCT	1 cut(s)	501
AfiI	CCNNNNNNNGG	8 cut(s)	95, 308, 433, 434, 569, 1726, 1727, 2261
AflII	CTTAAG	2 cut(s)	647, 2267
AflIII	ACRYGT	1 cut(s)	2004
AjnI	CCWGG	3 cut(s)	261, 1787, 2146
AjuI	GAANNNNNNNTTGG	6 cut(s)	401, 433, 940, 972, 996, 1028
AleI	CACNNNNGTG	1 cut(s)	1296
Alw21I	GWGCWC	2 cut(s)	29, 114
Alw26I	GTCTC	6 cut(s)	75, 779, 1039, 1076, 1790, 2225
AlwI	GGATC	5 cut(s)	230, 708, 1384, 1627, 1848
AlwNI	CAGNNNCTG	2 cut(s)	377, 1745
Ama87I	CYCGRG	1 cut(s)	1844
Aor51HI	AGCGCT	1 cut(s)	501
AoxI	GGCC	8 cut(s)	97, 354, 996, 1425, 1458, 1741, 1818, 1941
ApaI	GGGCCC	1 cut(s)	101
ApoI	RAATTY	3 cut(s)	449, 1134, 2077
AspLEI	GCGC	1 cut(s)	502
AspS9I	GGNCC	9 cut(s)	97, 98, 437, 533, 997, 1742, 1764, 1941, 2144
AsuC2I	CCSGG	1 cut(s)	220
AsuHPI	GGTGA	5 cut(s)	335, 1252, 1664, 2059, 2302
AvaI	CYCGRG	1 cut(s)	1844
AvaII	GGWCC	4 cut(s)	437, 533, 1764, 2144
BaeGI	GKGCMC	1 cut(s)	101
BalI	TGGCCA	2 cut(s)	356, 1820
BanII	GRGCYC	3 cut(s)	101, 114, 687
BbsI	GAAGAC	3 cut(s)	183, 1046, 2101
Bbv12I	GWGCWC	2 cut(s)	29, 114
BbvCI	CCTCAGC	1 cut(s)	2286
BccI	CCATC	8 cut(s)	134, 202, 203, 938, 1760, 1829, 2048, 2188
BceAI	ACGGC	2 cut(s)	1398, 1928
BcgI	CGANNNNNNTGC	2 cut(s)	2036, 2070
BciT130I	CCWGG	3 cut(s)	263, 1789, 2148
BciVI	GTATCC	1 cut(s)	505
BclI	TGATCA	1 cut(s)	2064
BcnI	CCSGG	1 cut(s)	220
BcoDI	GTCTC	6 cut(s)	75, 779, 1039, 1076, 1790, 2225
BfaI	CTAG	3 cut(s)	11, 605, 749
BfmI	CTRYAG	1 cut(s)	1206
BfoI	RGCGCY	1 cut(s)	503
BfrI	CTTAAG	2 cut(s)	647, 2267
BfuAI	ACCTGC	1 cut(s)	814
BfuI	GTATCC	1 cut(s)	505
BglII	AGATCT	2 cut(s)	7, 1434
BmcAI	AGTACT	1 cut(s)	1559
Bme1390I	CCNGG	4 cut(s)	220, 263, 1789, 2148
Bme18I	GGWCC	4 cut(s)	437, 533, 1764, 2144
BmeT110I	CYCGRG	1 cut(s)	1844
BmgT120I	GGNCC	9 cut(s)	97, 98, 437, 533, 997, 1742, 1764, 1941, 2144
BmiI	GGNNCC	4 cut(s)	99, 100, 438, 1533
BmrFI	CCNGG	4 cut(s)	220, 263, 1789, 2148
BmrI	ACTGGG	1 cut(s)	515
BmsI	GCATC	4 cut(s)	205, 724, 2044, 2205
BmuI	ACTGGG	1 cut(s)	515
BoxI	GACNNNNGTC	1 cut(s)	1293
BpiI	GAAGAC	3 cut(s)	183, 1046, 2101
BpmI	CTGGAG	1 cut(s)	678
Bpu10I	CCTNAGC	1 cut(s)	2286
BpuEI	CTTGAG	5 cut(s)	15, 98, 908, 1180, 2249
BpuMI	CCSGG	1 cut(s)	220
BsaHI	GRCGYC	2 cut(s)	68, 444
BsaI	GGTCTC	1 cut(s)	779
BsaJI	CCNNGG	4 cut(s)	123, 351, 1235, 1787
BsaWI	WCCGGW	1 cut(s)	2202
Bsc4I	CCNNNNNNNGG	8 cut(s)	95, 308, 433, 434, 569, 1726, 1727, 2261
Bse1I	ACTGG	4 cut(s)	521, 536, 1766, 2349
Bse3DI	GCAATG	3 cut(s)	288, 989, 1207
BseBI	CCWGG	3 cut(s)	263, 1789, 2148
BseDI	CCNNGG	4 cut(s)	123, 351, 1235, 1787
BseGI	GGATG	7 cut(s)	145, 1186, 1752, 1834, 2031, 2059, 2160
BseLI	CCNNNNNNNGG	8 cut(s)	95, 308, 433, 434, 569, 1726, 1727, 2261
BseMI	GCAATG	3 cut(s)	288, 989, 1207
BseNI	ACTGG	4 cut(s)	521, 536, 1766, 2349
BseRI	GAGGAG	3 cut(s)	1457, 1460, 2204
BseSI	GKGCMC	1 cut(s)	101
BseYI	CCCAGC	1 cut(s)	236
BsgI	GTGCAG	1 cut(s)	1110
Bsh1236I	CGCG	1 cut(s)	2115
BshFI	GGCC	8 cut(s)	99, 356, 998, 1427, 1460, 1743, 1820, 1943
BsiHKAI	GWGCWC	2 cut(s)	29, 114
BsiHKCI	CYCGRG	1 cut(s)	1844
BsiSI	CCGG	3 cut(s)	219, 765, 2203
BslFI	GGGAC	2 cut(s)	410, 1216
BslI	CCNNNNNNNGG	8 cut(s)	95, 308, 433, 434, 569, 1726, 1727, 2261
BsmAI	GTCTC	6 cut(s)	75, 779, 1039, 1076, 1790, 2225
BsmBI	CGTCTC	1 cut(s)	75
BsmFI	GGGAC	2 cut(s)	410, 1216
BsmI	GAATGC	3 cut(s)	725, 1737, 1948
BsnI	GGCC	8 cut(s)	99, 356, 998, 1427, 1460, 1743, 1820, 1943
Bso31I	GGTCTC	1 cut(s)	779
BsoBI	CYCGRG	1 cut(s)	1844
Bsp120I	GGGCCC	1 cut(s)	97
Bsp1286I	GDGCHC	4 cut(s)	29, 101, 114, 687
Bsp19I	CCATGG	1 cut(s)	351
BspACI	CCGC	5 cut(s)	809, 1663, 1717, 2113, 2335
BspANI	GGCC	8 cut(s)	99, 356, 998, 1427, 1460, 1743, 1820, 1943
BspFNI	CGCG	1 cut(s)	2115
BspHI	TCATGA	1 cut(s)	21
BspLI	GGNNCC	4 cut(s)	99, 100, 438, 1533
BspMAI	CTGCAG	1 cut(s)	1210
BspMI	ACCTGC	1 cut(s)	814
BspPI	GGATC	5 cut(s)	230, 708, 1384, 1627, 1848
BspQI	GCTCTTC	1 cut(s)	1494
BspTI	CTTAAG	2 cut(s)	647, 2267
BspTNI	GGTCTC	1 cut(s)	779
BsrDI	GCAATG	3 cut(s)	288, 989, 1207
BsrI	ACTGG	4 cut(s)	521, 536, 1766, 2349
BssECI	CCNNGG	4 cut(s)	123, 351, 1235, 1787
BssNI	GRCGYC	2 cut(s)	68, 444
BssT1I	CCWWGG	3 cut(s)	123, 351, 1235
Bst2UI	CCWGG	3 cut(s)	263, 1789, 2148
Bst4CI	ACNGT	4 cut(s)	1295, 1594, 1681, 2261
Bst6I	CTCTTC	2 cut(s)	606, 1494
BstACI	GRCGYC	2 cut(s)	68, 444
BstAFI	CTTAAG	2 cut(s)	647, 2267
BstAPI	GCANNNNNTGC	1 cut(s)	2306
BstC8I	GCNNGC	8 cut(s)	723, 996, 1116, 1717, 1923, 2220, 2298, 2346
BstDSI	CCRYGG	1 cut(s)	351
BstEII	GGTNACC	1 cut(s)	1240
BstF5I	GGATG	7 cut(s)	145, 1186, 1752, 1834, 2031, 2059, 2160
BstFNI	CGCG	1 cut(s)	2115
BstH2I	RGCGCY	1 cut(s)	503
BstHHI	GCGC	1 cut(s)	502
BstMAI	GTCTC	6 cut(s)	75, 779, 1039, 1076, 1790, 2225
BstMWI	GCNNNNNNNGC	8 cut(s)	659, 1014, 1124, 1214, 1271, 1339, 2306, 2315
BstNI	CCWGG	3 cut(s)	263, 1789, 2148
BstNSI	RCATGY	1 cut(s)	2008
BstPAI	GACNNNNGTC	1 cut(s)	1293
BstPI	GGTNACC	1 cut(s)	1240
BstSCI	CCNGG	4 cut(s)	218, 261, 1787, 2146
BstSFI	CTRYAG	1 cut(s)	1206
BstSLI	GKGCMC	1 cut(s)	101
BstUI	CGCG	1 cut(s)	2115
BstV2I	GAAGAC	3 cut(s)	183, 1046, 2101
BstX2I	RGATCY	5 cut(s)	7, 700, 1434, 1619, 1840
BstYI	RGATCY	5 cut(s)	7, 700, 1434, 1619, 1840
BsuI	GTATCC	1 cut(s)	505
BsuRI	GGCC	8 cut(s)	99, 356, 998, 1427, 1460, 1743, 1820, 1943
BtgI	CCRYGG	1 cut(s)	351
BtsCI	GGATG	7 cut(s)	145, 1186, 1752, 1834, 2031, 2059, 2160
BtsI	GCAGTG	4 cut(s)	157, 276, 846, 1059
BtsIMutI	CAGTG	8 cut(s)	157, 276, 543, 729, 846, 1059, 2311, 2356
BveI	ACCTGC	1 cut(s)	814
Cac8I	GCNNGC	8 cut(s)	723, 996, 1116, 1717, 1923, 2220, 2298, 2346
CaiI	CAGNNNCTG	2 cut(s)	377, 1745
CciI	TCATGA	1 cut(s)	21
CfoI	GCGC	1 cut(s)	502
Cfr13I	GGNCC	9 cut(s)	97, 98, 437, 533, 997, 1742, 1764, 1941, 2144
CseI	GACGC	1 cut(s)	57
CsiI	ACCWGGT	1 cut(s)	261
Csp6I	GTAC	4 cut(s)	803, 987, 1558, 2281
CspCI	CAANNNNNGTGG	2 cut(s)	1354, 1389
CviQI	GTAC	4 cut(s)	803, 987, 1558, 2281
DraIII	CACNNNGTG	1 cut(s)	2306
DrdI	GACNNNNNNGTC	1 cut(s)	1514
DseDI	GACNNNNNNGTC	1 cut(s)	1514
EaeI	YGGCCR	2 cut(s)	354, 1818
Eam1104I	CTCTTC	2 cut(s)	606, 1494
EarI	CTCTTC	2 cut(s)	606, 1494
Ecl136II	GAGCTC	1 cut(s)	112
Eco130I	CCWWGG	3 cut(s)	123, 351, 1235
Eco24I	GRGCYC	3 cut(s)	101, 114, 687
Eco31I	GGTCTC	1 cut(s)	779
Eco32I	GATATC	1 cut(s)	86
Eco47I	GGWCC	4 cut(s)	437, 533, 1764, 2144
Eco47III	AGCGCT	1 cut(s)	501
Eco53kI	GAGCTC	1 cut(s)	112
Eco57I	CTGAAG	1 cut(s)	1478
Eco88I	CYCGRG	1 cut(s)	1844
Eco91I	GGTNACC	1 cut(s)	1240
EcoICRI	GAGCTC	1 cut(s)	112
EcoO109I	RGGNCCY	4 cut(s)	98, 437, 997, 1742
EcoO65I	GGTNACC	1 cut(s)	1240
EcoRI	GAATTC	1 cut(s)	2077
EcoRII	CCWGG	3 cut(s)	261, 1787, 2146
EcoRV	GATATC	1 cut(s)	86
EcoT14I	CCWWGG	3 cut(s)	123, 351, 1235
EcoT38I	GRGCYC	3 cut(s)	101, 114, 687
ErhI	CCWWGG	3 cut(s)	123, 351, 1235
Esp3I	CGTCTC	1 cut(s)	75
FalI	AAGNNNNNCTT	8 cut(s)	107, 139, 890, 922, 911, 943, 1011, 1043
FaqI	GGGAC	2 cut(s)	410, 1216
FauI	CCCGC	1 cut(s)	1724
FauNDI	CATATG	1 cut(s)	457
FbaI	TGATCA	1 cut(s)	2064
FokI	GGATG	7 cut(s)	152, 1193, 1739, 1841, 2018, 2066, 2147
FriOI	GRGCYC	3 cut(s)	101, 114, 687
FspBI	CTAG	3 cut(s)	11, 605, 749
GlaI	GCGC	1 cut(s)	501
GsaI	CCCAGC	1 cut(s)	240
GsuI	CTGGAG	1 cut(s)	678
HaeII	RGCGCY	1 cut(s)	503
HaeIII	GGCC	8 cut(s)	99, 356, 998, 1427, 1460, 1743, 1820, 1943
HapII	CCGG	3 cut(s)	219, 765, 2203
HgaI	GACGC	1 cut(s)	57
HhaI	GCGC	1 cut(s)	502
Hin1I	GRCGYC	2 cut(s)	68, 444
Hin6I	GCGC	1 cut(s)	500
HinP1I	GCGC	1 cut(s)	500
HincII	GTYRAC	3 cut(s)	1147, 1780, 2251
HindII	GTYRAC	3 cut(s)	1147, 1780, 2251
HindIII	AAGCTT	2 cut(s)	1340, 1490
HinfI	GANTC	9 cut(s)	128, 670, 707, 752, 823, 843, 1184, 1993, 2272
HpaI	GTTAAC	1 cut(s)	1147
HpaII	CCGG	3 cut(s)	219, 765, 2203
HphI	GGTGA	5 cut(s)	335, 1252, 1664, 2059, 2302
Hpy166II	GTNNAC	3 cut(s)	1147, 1780, 2251
Hpy8I	GTNNAC	3 cut(s)	1147, 1780, 2251
Hpy99I	CGWCG	1 cut(s)	1299
HpyAV	CCTTC	7 cut(s)	832, 942, 998, 1273, 1398, 1720, 1889
HpyCH4III	ACNGT	4 cut(s)	1295, 1594, 1681, 2261
HpyCH4IV	ACGT	2 cut(s)	444, 1968
HpyF10VI	GCNNNNNNNGC	8 cut(s)	659, 1014, 1124, 1214, 1271, 1339, 2306, 2315
HpySE526I	ACGT	2 cut(s)	444, 1968
Hsp92I	GRCGYC	2 cut(s)	68, 444
HspAI	GCGC	1 cut(s)	500
Ksp22I	TGATCA	1 cut(s)	2064
KspAI	GTTAAC	1 cut(s)	1147
LguI	GCTCTTC	1 cut(s)	1494
LmnI	GCTCC	4 cut(s)	659, 1531, 1567, 2103
LweI	GCATC	4 cut(s)	205, 724, 2044, 2205
MabI	ACCWGGT	1 cut(s)	261
MaeI	CTAG	3 cut(s)	11, 605, 749
MaeII	ACGT	2 cut(s)	444, 1968
MaeIII	GTNAC	6 cut(s)	538, 844, 902, 1172, 1240, 1747
MboII	GAAGA	8 cut(s)	170, 183, 593, 1046, 1340, 1348, 1511, 2101
MflI	RGATCY	5 cut(s)	7, 700, 1434, 1619, 1840
MhlI	GDGCHC	4 cut(s)	29, 101, 114, 687
MlsI	TGGCCA	2 cut(s)	356, 1820
MluCI	AATT	8 cut(s)	449, 894, 1134, 1353, 1473, 1571, 1872, 2077
MluNI	TGGCCA	2 cut(s)	356, 1820
MlyI	GAGTC	4 cut(s)	817, 852, 1193, 2266
MmeI	TCCRAC	3 cut(s)	61, 293, 2040
Mox20I	TGGCCA	2 cut(s)	356, 1820
MscI	TGGCCA	2 cut(s)	356, 1820
MseI	TTAA	9 cut(s)	648, 893, 897, 1077, 1146, 1344, 1464, 2208, 2268
MslI	CAYNNNNRTG	5 cut(s)	281, 1179, 1296, 2003, 2048
Msp20I	TGGCCA	2 cut(s)	356, 1820
MspA1I	CMGCKG	1 cut(s)	656
MspCI	CTTAAG	2 cut(s)	647, 2267
MspI	CCGG	3 cut(s)	219, 765, 2203
MspR9I	CCNGG	4 cut(s)	220, 263, 1789, 2148
Mva1269I	GAATGC	3 cut(s)	725, 1737, 1948
MvaI	CCWGG	3 cut(s)	263, 1789, 2148
MvnI	CGCG	1 cut(s)	2115
MwoI	GCNNNNNNNGC	8 cut(s)	659, 1014, 1124, 1214, 1271, 1339, 2306, 2315
NciI	CCSGG	1 cut(s)	220
NcoI	CCATGG	1 cut(s)	351
NdeI	CATATG	1 cut(s)	457
NlaIV	GGNNCC	4 cut(s)	99, 100, 438, 1533
NmuCI	GTSAC	5 cut(s)	538, 844, 902, 1172, 1240
NspI	RCATGY	1 cut(s)	2008
OliI	CACNNNNGTG	1 cut(s)	1296
PacI	TTAATTAA	1 cut(s)	897
PaeR7I	CTCGAG	1 cut(s)	1844
PagI	TCATGA	1 cut(s)	21
PciI	ACATGT	1 cut(s)	2004
PciSI	GCTCTTC	1 cut(s)	1494
PctI	GAATGC	3 cut(s)	725, 1737, 1948
PfeI	GAWTC	5 cut(s)	128, 670, 707, 752, 1993
PflMI	CCANNNNNTGG	2 cut(s)	308, 2261
PleI	GAGTC	4 cut(s)	817, 851, 1192, 2266
PpsI	GAGTC	4 cut(s)	817, 851, 1192, 2266
PpuMI	RGGWCCY	1 cut(s)	437
PscI	ACATGT	1 cut(s)	2004
PshAI	GACNNNNGTC	1 cut(s)	1293
Psp124BI	GAGCTC	1 cut(s)	114
Psp1406I	AACGTT	1 cut(s)	1968
Psp5II	RGGWCCY	1 cut(s)	437
Psp6I	CCWGG	3 cut(s)	261, 1787, 2146
PspEI	GGTNACC	1 cut(s)	1240
PspFI	CCCAGC	1 cut(s)	236
PspGI	CCWGG	3 cut(s)	261, 1787, 2146
PspN4I	GGNNCC	4 cut(s)	99, 100, 438, 1533
PspOMI	GGGCCC	1 cut(s)	97
PspPI	GGNCC	9 cut(s)	97, 98, 437, 533, 997, 1742, 1764, 1941, 2144
PspPPI	RGGWCCY	1 cut(s)	437
PstI	CTGCAG	1 cut(s)	1210
PstNI	CAGNNNCTG	2 cut(s)	377, 1745
PsuI	RGATCY	5 cut(s)	7, 700, 1434, 1619, 1840
PvuII	CAGCTG	1 cut(s)	656
RsaI	GTAC	4 cut(s)	804, 988, 1559, 2282
RsaNI	GTAC	4 cut(s)	803, 987, 1558, 2281
RseI	CAYNNNNRTG	5 cut(s)	281, 1179, 1296, 2003, 2048
SacI	GAGCTC	1 cut(s)	114
SapI	GCTCTTC	1 cut(s)	1494
SaqAI	TTAA	9 cut(s)	648, 893, 897, 1077, 1146, 1344, 1464, 2208, 2268
Sau96I	GGNCC	9 cut(s)	97, 98, 437, 533, 997, 1742, 1764, 1941, 2144
ScaI	AGTACT	1 cut(s)	1559
SchI	GAGTC	4 cut(s)	817, 852, 1193, 2266
ScrFI	CCNGG	4 cut(s)	220, 263, 1789, 2148
SduI	GDGCHC	4 cut(s)	29, 101, 114, 687
SexAI	ACCWGGT	1 cut(s)	261
SfaNI	GCATC	4 cut(s)	205, 724, 2044, 2205
SfcI	CTRYAG	1 cut(s)	1206
Sfr274I	CTCGAG	1 cut(s)	1844
SinI	GGWCC	4 cut(s)	437, 533, 1764, 2144
SlaI	CTCGAG	1 cut(s)	1844
SmiMI	CAYNNNNRTG	5 cut(s)	281, 1179, 1296, 2003, 2048
SmlI	CTYRAG	8 cut(s)	30, 113, 647, 887, 1195, 1844, 2228, 2267
SmoI	CTYRAG	8 cut(s)	30, 113, 647, 887, 1195, 1844, 2228, 2267
Sse9I	AATT	8 cut(s)	449, 894, 1134, 1353, 1473, 1571, 1872, 2077
SsiI	CCGC	5 cut(s)	809, 1663, 1717, 2113, 2335
SspMI	CTAG	3 cut(s)	11, 605, 749
SstI	GAGCTC	1 cut(s)	114
StyD4I	CCNGG	4 cut(s)	218, 261, 1787, 2146
StyI	CCWWGG	3 cut(s)	123, 351, 1235
TaaI	ACNGT	4 cut(s)	1295, 1594, 1681, 2261
TaiI	ACGT	2 cut(s)	447, 1971
TaqI	TCGA	3 cut(s)	1624, 1845, 2046
TaqII	GACCGA	1 cut(s)	2095
TasI	AATT	8 cut(s)	449, 894, 1134, 1353, 1473, 1571, 1872, 2077
TatI	WGTACW	2 cut(s)	986, 1557
TauI	GCSGC	1 cut(s)	1665
TfiI	GAWTC	5 cut(s)	128, 670, 707, 752, 1993
Tru1I	TTAA	9 cut(s)	648, 893, 897, 1077, 1146, 1344, 1464, 2208, 2268
Tru9I	TTAA	9 cut(s)	648, 893, 897, 1077, 1146, 1344, 1464, 2208, 2268
TscAI	CASTG	8 cut(s)	157, 283, 543, 736, 853, 1066, 2311, 2356
TseFI	GTSAC	5 cut(s)	538, 844, 902, 1172, 1240
Tsp45I	GTSAC	5 cut(s)	538, 844, 902, 1172, 1240
TspDTI	ATGAA	7 cut(s)	409, 654, 1113, 1514, 1739, 1845, 1941
TspRI	CASTG	8 cut(s)	157, 283, 543, 736, 853, 1066, 2311, 2356
Van91I	CCANNNNNTGG	2 cut(s)	308, 2261
Vha464I	CTTAAG	2 cut(s)	647, 2267
VpaK11BI	GGWCC	4 cut(s)	437, 533, 1764, 2144
XapI	RAATTY	3 cut(s)	449, 1134, 2077
XceI	RCATGY	1 cut(s)	2008
XcmI	CCANNNNNNNNNTGG	3 cut(s)	309, 694, 2047
XhoI	CTCGAG	1 cut(s)	1844
XspI	CTAG	3 cut(s)	11, 605, 749
ZraI	GACGTC	1 cut(s)	445
ZrmI	AGTACT	1 cut(s)	1559

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.