Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0004023

Physical Location & Seq

Genomic Coordinates Reverse (-)

547065 .. 551204

Gene Structure 4140 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom12g00610.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2358 bp

ATGAAATTGCCTGCTTTAAACATTAGATCTCTAAGTACGAAGATTTTGTGTTTAAGAGAAATGGCGTCTCACATTGTTGGATATCCCCGTATGGGCCCTAAGAGAGAGCTCAAGTTTGCTCTTGAATCTTTCTGGGATGGCAAGAGCAGTGCTGAGGACTTGCAGAAGGTGTCTGCTGATCTCAGATCTTCCATCTGGAAGCAGATGGCCGATGCCGGGATCAAGCACATTCCCAGCAACACCTTCTCATACTACGATCAGGTGCTTGACACCACCGCATTGCTTGGCGCTGTCCCACCAAGATATGGATGGAACGGCGGTGAGATTGGATTCGATACATACTTCTCCATGGCCAGAGGAAATGCCTCAGTTCCTGCTATGGAAATGACCAAGTGGTTTGATACCAACTACCATTTCATTGTCCCTGAGTTGGGCCCTGATGTGAACTTCTCTTATGCTTCTCACAAGGCTGTTGAGGAATACAAGGAGGCTAAGGCGCTTGGAGTGGATACTGTCCCAGTCCTTGTTGGCCCAGTGTCATACTTGTTGCTGTCCAAGCCAGCCAAGGGTGTTGAGAAGACCTTTTCTCTTCTCTCTCTTCTTCCTAAAATTCTTCCTATCTATAAGGAAGTTGTATCTGAGCTTAAGGCAGCTGGAGCTTCATGGATTCAATTTGATGAGCCCACACTTGTTTTGGATCTTGAATCCGACAAGTTGCAAGCATTCACTGGTGCCTACTCTGAACTGGAGTCAGCTCTATCTGGCTTGAATGTTCTTATTGAAACCTACTTTGCTGATGTTCCTGCTGCGGCATTCAAGACCCTCACTAGCTTGAAGGGTGTCACTGCTTATGGTTTTGATTTGGTCCGTGGTACCAAGACCCTGGATTTGATTAAGAGTGAATTCCCCAAGGGAAAGTACCTCTTTGCTGGTGTGGTTGATGGAAGGAACATCTGGGCTAATGATCTTTCTGCCTCTCTAAGCACATTGAAGACCCTTGAAGGCATTGTTGGAAAAGATAAACTTGTTGTCTCCACCTCCACCTCTCTTCTTCACACCGCTGTTGACCTTGTCAATGAGACCAAGCTCGACCAGGAAATTAAGTCATGGTTGGCTTTTGCTGCCCAGAAAATTATTGAAGTTAATGCTTTGGCCAAGGCCTTGGCTGGTCACAAGGACGAGGCATTTTTCTCTGCTAATGCCGCTGCCCAGGCTTCAAGGAAGTCCTCCCCAAGGGTGACCAATGAGGCTGTTCAGAAGGCTGCTGCTGCTTTGAAGGGTTCTGACCACCGTCGTGCTACCAATGTTAGTGCTAGACTCGATGCTCAACAGAAGAAGCTTAACCTTCCAATTCTCCCAACCACCACCATTGGATCGTTCCCTCAGACCATTGAACTCAGGAGGGTCCGCCGTGAGTACAAGGCCAAGAAGATTTCTGAGGAGGAATATGTCAAGGCCATCAAGGAAGAAATTTATAAGGTTGTTAAACTTCAGGAAGAGCTAGACATTGATGTCCTTGTTCATGGAGAGCCTGAGAGGAACGATATGGTTGAGTACTTCGGAGAGCAATTATCTGGCTTTGCCTTTACCGCCAATGGGTGGGTGCAATCATATGGATCTCGATGTGTGAAGCCACCAATCATCTACGGTGATGTGAGCCGCCCCAAGCCTATGACTGTATTCTGGTCATCTGCTGCTCAGAGCATGACTGCCCGCCCAATGAAGGGTATGCTTACCGGCCCCGTCACTATCCTGAACTGGTCTTTCGTCAGAAATGACCAGCCCAGATTCGAGACCACCTACCAGATTGCTTTGGCCATCAAGGATGAAGTGGAGGATCTTGAGAAGGCTGGTATCAGTGTTATTCAGATCGATGAGGCTGCTCTGAGAGAGGGATTGCCTCTAAGGAAGTCTGAACATGCTTTCTACTTGGATTGGGCAGTGCATTCCTTCAGGATCACCAACAGTTGTGTCCAGGACACTACCCAGATTCACACTCACATGTGCTACTCCAACTTCAACGACATCATCCACTCAATCATCGACATGGACGCTGATGTAATCACCATCGAGAACTCTCGTTCTGATGAGAAGCTCCTGTCAGTCTTCCGTGAGGGAGTCAAGTACGGTGCTGGAATTGGCCCTGGTGTGTATGACATCCACTCTCCCAGAATACCATCAACTGACGAGATTGCTGACCGGATCAACAAGATGCTTGCAGTGCTGGAGACCAACATCTTGTGGGTGAACCCCGACTGTGGGCTCAAGACCCGCAAGTACACAGAAGTGAAGCCGGCGCTCAGCAACATGGTTGCTGCCGCCAAGCTCCTCCGCACCGAGCTCGCCAGTGCCAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000096 GO:0000097 GO:0003674 GO:0003824 GO:0003871 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005777 GO:0005829 GO:0005886 GO:0005911 GO:0006082 GO:0006520 GO:0006555 GO:0006790 GO:0006807 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0008168 GO:0008172 GO:0008270 GO:0008652 GO:0008705 GO:0009058 GO:0009066 GO:0009067 GO:0009086 GO:0009506 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009651 GO:0009941 GO:0009987 GO:0010035 GO:0010038 GO:0010043 GO:0016020 GO:0016053 GO:0016740 GO:0016741 GO:0019752 GO:0030054 GO:0031090 GO:0031967 GO:0031975 GO:0032259 GO:0042084 GO:0042085 GO:0042221 GO:0042579 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044272 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046686 GO:0046872 GO:0046914 GO:0048046 GO:0050667 GO:0050896 GO:0055044 GO:0071704 GO:0071944 GO:0098588 GO:0098805 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00270 ko00450 ko01100 ko01110 ko01230 map00270 map00450 map01100 map01110 map01230

Pfam Domains

Protein Families

Meth_synt_1 Meth_synt_2

Protein Analysis

786

Amino Acids

86.91

Weight (kDa)

6.58

Isoelectric Point (pI)

35.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Meth_synt_1	PF08267	23 - 335	1.3e-117	Cobalamin-independent synthase, N-terminal domain
Meth_synt_2	PF01717	452 - 775	5.7e-159	Cobalamin-independent synthase, Catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0004023)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G03780 AT3G03780 AT3G03780 AT5G17920 AT5G17920
fragaria_vesca	FvH4_1g28390 FvH4_1g28390 FvH4_1g28390 FvH4_1g28390 FvH4_2g41260 FvH4_2g41260 FvH4_2g41260 FvH4_2g41260 FvH4_2g41260 FvH4_2g41260
malus_domestica	MD12G1008100.v1.1 MD14G1005500.v1.1
prunus_persica	Prupe.7G009200_v2.0.a1 Prupe.7G009200_v2.0.a1 Prupe.7G009200_v2.0.a1
pyrus_communis	pycom12g00610 pycom14g00410
rosa_chinensis	RchiOBHm_Chr3g0496511
rosa_laevigata	RLG00000022522
rosa_multiflora	Rmu_sc0000620.1_g000008
rosa_roxburghii	Rroxscaffold_6G00389060
rosa_rugosa	Rorug03G0283400 Rorug03G0283500
rosa_samantha	Rh3AG329600 Rh3BG365100 Rh3CG362500 Rh3DG365600
rosa_wichuraiana	Rw3G028830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1476
AasI	GACNNNNNNGTC	1 cut(s)	1511
Acc65I	GGTACC	1 cut(s)	872
AccB1I	GGYRCC	2 cut(s)	731, 872
AccB7I	CCANNNNNTGG	2 cut(s)	305, 1599
AclWI	GGATC	7 cut(s)	227, 705, 1381, 1624, 1845, 1964, 2210
AcoI	YGGCCR	4 cut(s)	207, 351, 1152, 1815
AcsI	RAATTY	3 cut(s)	609, 902, 1470
AcuI	CTGAAG	2 cut(s)	1475, 1936
AcyI	GRCGYC	1 cut(s)	65
AfaI	GTAC	7 cut(s)	37, 874, 920, 1418, 1556, 2126, 2279
AflII	CTTAAG	1 cut(s)	644
AflIII	ACRYGT	1 cut(s)	2001
AjnI	CCWGG	5 cut(s)	882, 1092, 1209, 1974, 2143
AjuI	GAANNNNNNNTTGG	4 cut(s)	398, 430, 993, 1025
AleI	CACNNNNGTG	2 cut(s)	1293, 2285
Alw21I	GWGCWC	2 cut(s)	111, 2343
Alw26I	GTCTC	5 cut(s)	72, 1036, 1073, 1787, 2222
AlwI	GGATC	7 cut(s)	227, 705, 1381, 1624, 1845, 1964, 2210
AlwNI	CAGNNNCTG	1 cut(s)	374
ApaI	GGGCCC	2 cut(s)	98, 437
ApoI	RAATTY	3 cut(s)	609, 902, 1470
ArsI	GACNNNNNNTTYG	2 cut(s)	871, 903
Asp718I	GGTACC	1 cut(s)	872
AspLEI	GCGC	3 cut(s)	290, 499, 2299
AspS9I	GGNCC	9 cut(s)	94, 95, 433, 434, 530, 865, 1405, 1739, 2141
AsuC2I	CCSGG	1 cut(s)	217
AsuHPI	GGTGA	6 cut(s)	332, 1249, 1661, 1951, 2056, 2257
AvaII	GGWCC	2 cut(s)	865, 1405
BaeGI	GKGCMC	2 cut(s)	98, 437
BalI	TGGCCA	3 cut(s)	353, 1154, 1817
BanI	GGYRCC	2 cut(s)	731, 872
BanII	GRGCYC	6 cut(s)	98, 111, 437, 684, 2265, 2343
BbsI	GAAGAC	3 cut(s)	584, 998, 2098
Bbv12I	GWGCWC	2 cut(s)	111, 2343
BbvCI	CCTCAGC	1 cut(s)	153
BccI	CCATC	9 cut(s)	131, 199, 200, 303, 935, 1466, 1826, 2075, 2185
BceAI	ACGGC	2 cut(s)	331, 1395
BcgI	CGANNNNNNTGC	4 cut(s)	1862, 1896, 2323, 2357
BciT130I	CCWGG	5 cut(s)	884, 1094, 1211, 1976, 2145
BciVI	GTATCC	1 cut(s)	502
BcnI	CCSGG	1 cut(s)	217
BcoDI	GTCTC	5 cut(s)	72, 1036, 1073, 1787, 2222
BfaI	CTAG	3 cut(s)	828, 1314, 1502
BfoI	RGCGCY	3 cut(s)	291, 500, 2300
BfrI	CTTAAG	1 cut(s)	644
BfuI	GTATCC	1 cut(s)	502
BglII	AGATCT	2 cut(s)	26, 185
BlpI	GCTNAGC	1 cut(s)	2300
BmcAI	AGTACT	1 cut(s)	1556
Bme1390I	CCNGG	6 cut(s)	217, 884, 1094, 1211, 1976, 2145
Bme18I	GGWCC	2 cut(s)	865, 1405
BmgT120I	GGNCC	9 cut(s)	94, 95, 433, 434, 530, 865, 1405, 1739, 2141
BmiI	GGNNCC	6 cut(s)	96, 435, 733, 874, 1406, 1741
BmrFI	CCNGG	6 cut(s)	217, 884, 1094, 1211, 1976, 2145
BmrI	ACTGGG	2 cut(s)	512, 527
BmsI	GCATC	3 cut(s)	202, 1312, 2202
BmuI	ACTGGG	2 cut(s)	512, 527
BoxI	GACNNNNGTC	1 cut(s)	1290
BpiI	GAAGAC	3 cut(s)	584, 998, 2098
BpmI	CTGGAG	3 cut(s)	675, 767, 2246
Bpu10I	CCTNAGC	2 cut(s)	153, 492
Bpu1102I	GCTNAGC	1 cut(s)	2300
BpuEI	CTTGAG	3 cut(s)	95, 1862, 2249
BpuMI	CCSGG	1 cut(s)	217
Bsa29I	ATCGAT	1 cut(s)	1872
BsaHI	GRCGYC	1 cut(s)	65
BsaI	GGTCTC	3 cut(s)	1073, 1787, 2222
BsaWI	WCCGGW	1 cut(s)	2199
Bse118I	RCCGGY	2 cut(s)	1736, 2293
Bse1I	ACTGG	6 cut(s)	518, 533, 733, 750, 1763, 2346
Bse3DI	GCAATG	1 cut(s)	278
BseBI	CCWGG	5 cut(s)	884, 1094, 1211, 1976, 2145
BseCI	ATCGAT	1 cut(s)	1872
BseGI	GGATG	5 cut(s)	142, 314, 1831, 2028, 2157
BseMI	GCAATG	1 cut(s)	278
BseNI	ACTGG	6 cut(s)	518, 533, 733, 750, 1763, 2346
BseRI	GAGGAG	2 cut(s)	1454, 2318
BseSI	GKGCMC	2 cut(s)	98, 437
BseYI	CCCAGC	1 cut(s)	233
BshNI	GGYRCC	2 cut(s)	731, 872
BshVI	ATCGAT	1 cut(s)	1872
BsiHKAI	GWGCWC	2 cut(s)	111, 2343
BsiSI	CCGG	4 cut(s)	216, 1737, 2200, 2294
BslFI	GGGAC	3 cut(s)	278, 407, 500
BsmAI	GTCTC	5 cut(s)	72, 1036, 1073, 1787, 2222
BsmBI	CGTCTC	1 cut(s)	72
BsmFI	GGGAC	3 cut(s)	278, 407, 500
BsmI	GAATGC	3 cut(s)	722, 812, 1945
Bso31I	GGTCTC	3 cut(s)	1073, 1787, 2222
Bsp120I	GGGCCC	2 cut(s)	94, 433
Bsp1286I	GDGCHC	6 cut(s)	98, 111, 437, 684, 2265, 2343
Bsp1720I	GCTNAGC	1 cut(s)	2300
Bsp19I	CCATGG	1 cut(s)	348
BspDI	ATCGAT	1 cut(s)	1872
BspLI	GGNNCC	6 cut(s)	96, 435, 733, 874, 1406, 1741
BspPI	GGATC	7 cut(s)	227, 705, 1381, 1624, 1845, 1964, 2210
BspQI	GCTCTTC	1 cut(s)	1491
BspT107I	GGYRCC	2 cut(s)	731, 872
BspTI	CTTAAG	1 cut(s)	644
BspTNI	GGTCTC	3 cut(s)	1073, 1787, 2222
BsrDI	GCAATG	1 cut(s)	278
BsrFI	RCCGGY	2 cut(s)	1736, 2293
BsrI	ACTGG	6 cut(s)	518, 533, 733, 750, 1763, 2346
BssAI	RCCGGY	2 cut(s)	1736, 2293
BssNI	GRCGYC	1 cut(s)	65
BssT1I	CCWWGG	6 cut(s)	348, 564, 909, 1155, 1161, 1232
Bst2UI	CCWGG	5 cut(s)	884, 1094, 1211, 1976, 2145
Bst4CI	ACNGT	7 cut(s)	514, 1292, 1649, 1678, 1967, 2129, 2258
Bst6I	CTCTTC	4 cut(s)	594, 603, 1053, 1491
BstACI	GRCGYC	1 cut(s)	65
BstAFI	CTTAAG	1 cut(s)	644
BstC8I	GCNNGC	7 cut(s)	12, 561, 720, 1714, 2217, 2295, 2343
BstDSI	CCRYGG	2 cut(s)	348, 868
BstEII	GGTNACC	1 cut(s)	1237
BstENI	CCTNNNNNAGG	1 cut(s)	1231
BstF5I	GGATG	5 cut(s)	142, 314, 1831, 2028, 2157
BstH2I	RGCGCY	3 cut(s)	291, 500, 2300
BstHHI	GCGC	3 cut(s)	290, 499, 2299
BstMAI	GTCTC	5 cut(s)	72, 1036, 1073, 1787, 2222
BstMWI	GCNNNNNNNGC	8 cut(s)	556, 656, 1121, 1202, 1211, 1268, 1589, 2221
BstNI	CCWGG	5 cut(s)	884, 1094, 1211, 1976, 2145
BstNSI	RCATGY	2 cut(s)	1922, 2005
BstPAI	GACNNNNGTC	1 cut(s)	1290
BstPI	GGTNACC	1 cut(s)	1237
BstSCI	CCNGG	6 cut(s)	215, 882, 1092, 1209, 1974, 2143
BstSLI	GKGCMC	2 cut(s)	98, 437
BstV2I	GAAGAC	3 cut(s)	584, 998, 2098
BstX2I	RGATCY	5 cut(s)	26, 185, 697, 1616, 1837
BstXI	CCANNNNNNTGG	2 cut(s)	883, 1162
BstYI	RGATCY	5 cut(s)	26, 185, 697, 1616, 1837
Bsu15I	ATCGAT	1 cut(s)	1872
BsuI	GTATCC	1 cut(s)	502
BsuTUI	ATCGAT	1 cut(s)	1872
BtgI	CCRYGG	2 cut(s)	348, 868
BtsCI	GGATG	5 cut(s)	142, 314, 1831, 2028, 2157
BtsI	GCAGTG	4 cut(s)	154, 843, 1947, 2226
BtsIMutI	CAGTG	8 cut(s)	154, 540, 726, 843, 1864, 1947, 2226, 2353
Cac8I	GCNNGC	7 cut(s)	12, 561, 720, 1714, 2217, 2295, 2343
CaiI	CAGNNNCTG	1 cut(s)	374
CfoI	GCGC	3 cut(s)	290, 499, 2299
Cfr10I	RCCGGY	2 cut(s)	1736, 2293
Cfr13I	GGNCC	9 cut(s)	94, 95, 433, 434, 530, 865, 1405, 1739, 2141
ClaI	ATCGAT	1 cut(s)	1872
CseI	GACGC	2 cut(s)	54, 2060
Csp6I	GTAC	7 cut(s)	36, 873, 919, 1417, 1555, 2125, 2278
CspCI	CAANNNNNGTGG	2 cut(s)	1351, 1386
CviAII	CATG	9 cut(s)	349, 663, 1107, 1523, 1705, 1919, 2002, 2047, 2308
CviQI	GTAC	7 cut(s)	36, 873, 919, 1417, 1555, 2125, 2278
DraI	TTTAAA	1 cut(s)	18
DrdI	GACNNNNNNGTC	1 cut(s)	1511
DseDI	GACNNNNNNGTC	1 cut(s)	1511
EaeI	YGGCCR	4 cut(s)	207, 351, 1152, 1815
Eam1104I	CTCTTC	4 cut(s)	594, 603, 1053, 1491
EarI	CTCTTC	4 cut(s)	594, 603, 1053, 1491
EciI	GGCGGA	1 cut(s)	1397
Ecl136II	GAGCTC	2 cut(s)	109, 2341
Eco130I	CCWWGG	6 cut(s)	348, 564, 909, 1155, 1161, 1232
Eco147I	AGGCCT	1 cut(s)	1160
Eco24I	GRGCYC	6 cut(s)	98, 111, 437, 684, 2265, 2343
Eco31I	GGTCTC	3 cut(s)	1073, 1787, 2222
Eco32I	GATATC	1 cut(s)	83
Eco47I	GGWCC	2 cut(s)	865, 1405
Eco53kI	GAGCTC	2 cut(s)	109, 2341
Eco57I	CTGAAG	2 cut(s)	1475, 1936
Eco91I	GGTNACC	1 cut(s)	1237
EcoICRI	GAGCTC	2 cut(s)	109, 2341
EcoNI	CCTNNNNNAGG	1 cut(s)	1231
EcoO109I	RGGNCCY	2 cut(s)	95, 434
EcoO65I	GGTNACC	1 cut(s)	1237
EcoRI	GAATTC	1 cut(s)	902
EcoRII	CCWGG	5 cut(s)	882, 1092, 1209, 1974, 2143
EcoRV	GATATC	1 cut(s)	83
EcoT14I	CCWWGG	6 cut(s)	348, 564, 909, 1155, 1161, 1232
EcoT38I	GRGCYC	6 cut(s)	98, 111, 437, 684, 2265, 2343
ErhI	CCWWGG	6 cut(s)	348, 564, 909, 1155, 1161, 1232
Esp3I	CGTCTC	1 cut(s)	72
FaeI	CATG	9 cut(s)	352, 666, 1110, 1526, 1708, 1922, 2005, 2050, 2311
FalI	AAGNNNNNCTT	4 cut(s)	908, 940, 1008, 1040
FaqI	GGGAC	3 cut(s)	278, 407, 500
FatI	CATG	9 cut(s)	348, 662, 1106, 1522, 1704, 1918, 2001, 2046, 2307
FauI	CCCGC	2 cut(s)	1721, 2279
FauNDI	CATATG	1 cut(s)	1612
FokI	GGATG	5 cut(s)	149, 321, 1838, 2015, 2144
FriOI	GRGCYC	6 cut(s)	98, 111, 437, 684, 2265, 2343
FspBI	CTAG	3 cut(s)	828, 1314, 1502
GlaI	GCGC	3 cut(s)	289, 498, 2298
GsaI	CCCAGC	1 cut(s)	237
GsuI	CTGGAG	3 cut(s)	675, 767, 2246
HaeII	RGCGCY	3 cut(s)	291, 500, 2300
HapII	CCGG	4 cut(s)	216, 1737, 2200, 2294
HgaI	GACGC	2 cut(s)	54, 2060
HhaI	GCGC	3 cut(s)	290, 499, 2299
Hin1I	GRCGYC	1 cut(s)	65
Hin1II	CATG	9 cut(s)	352, 666, 1110, 1526, 1708, 1922, 2005, 2050, 2311
Hin6I	GCGC	3 cut(s)	288, 497, 2297
HinP1I	GCGC	3 cut(s)	288, 497, 2297
HincII	GTYRAC	1 cut(s)	1066
HindII	GTYRAC	1 cut(s)	1066
HindIII	AAGCTT	1 cut(s)	1337
HinfI	GANTC	9 cut(s)	125, 330, 667, 704, 749, 1317, 1788, 1990, 2118
HpaII	CCGG	4 cut(s)	216, 1737, 2200, 2294
HphI	GGTGA	6 cut(s)	332, 1249, 1661, 1951, 2056, 2257
Hpy166II	GTNNAC	4 cut(s)	445, 1066, 2248, 2280
Hpy8I	GTNNAC	4 cut(s)	445, 1066, 2248, 2280
Hpy99I	CGWCG	1 cut(s)	1296
HpyCH4III	ACNGT	7 cut(s)	514, 1292, 1649, 1678, 1967, 2129, 2258
HpyCH4V	TGCA	5 cut(s)	163, 718, 1606, 1945, 2219
HpyF10VI	GCNNNNNNNGC	8 cut(s)	556, 656, 1121, 1202, 1211, 1268, 1589, 2221
Hsp92I	GRCGYC	1 cut(s)	65
Hsp92II	CATG	9 cut(s)	352, 666, 1110, 1526, 1708, 1922, 2005, 2050, 2311
HspAI	GCGC	3 cut(s)	288, 497, 2297
KpnI	GGTACC	1 cut(s)	876
KroI	GCCGGC	1 cut(s)	2293
KroNI	GCCGGC	1 cut(s)	2295
LguI	GCTCTTC	1 cut(s)	1491
LmnI	GCTCC	3 cut(s)	656, 2100, 2331
LweI	GCATC	3 cut(s)	202, 1312, 2202
MaeI	CTAG	3 cut(s)	828, 1314, 1502
MaeIII	GTNAC	4 cut(s)	841, 1169, 1237, 1744
MflI	RGATCY	5 cut(s)	26, 185, 697, 1616, 1837
MhlI	GDGCHC	6 cut(s)	98, 111, 437, 684, 2265, 2343
MlsI	TGGCCA	3 cut(s)	353, 1154, 1817
MluNI	TGGCCA	3 cut(s)	353, 1154, 1817
MlyI	GAGTC	3 cut(s)	758, 1311, 2127
MmeI	TCCRAC	4 cut(s)	58, 732, 991, 2037
Mox20I	TGGCCA	3 cut(s)	353, 1154, 1817
MroNI	GCCGGC	1 cut(s)	2293
MscI	TGGCCA	3 cut(s)	353, 1154, 1817
MseI	TTAA	8 cut(s)	17, 53, 645, 894, 1101, 1143, 1341, 1485
MslI	CAYNNNNRTG	4 cut(s)	1293, 2000, 2045, 2285
Msp20I	TGGCCA	3 cut(s)	353, 1154, 1817
MspA1I	CMGCKG	3 cut(s)	653, 1061, 1205
MspCI	CTTAAG	1 cut(s)	644
MspI	CCGG	4 cut(s)	216, 1737, 2200, 2294
MspR9I	CCNGG	6 cut(s)	217, 884, 1094, 1211, 1976, 2145
Mva1269I	GAATGC	3 cut(s)	722, 812, 1945
MvaI	CCWGG	5 cut(s)	884, 1094, 1211, 1976, 2145
MwoI	GCNNNNNNNGC	8 cut(s)	556, 656, 1121, 1202, 1211, 1268, 1589, 2221
NaeI	GCCGGC	1 cut(s)	2295
NciI	CCSGG	1 cut(s)	217
NcoI	CCATGG	1 cut(s)	348
NdeI	CATATG	1 cut(s)	1612
NgoMIV	GCCGGC	1 cut(s)	2293
NlaIII	CATG	9 cut(s)	352, 666, 1110, 1526, 1708, 1922, 2005, 2050, 2311
NlaIV	GGNNCC	6 cut(s)	96, 435, 733, 874, 1406, 1741
NmuCI	GTSAC	4 cut(s)	841, 1169, 1237, 1744
NspI	RCATGY	2 cut(s)	1922, 2005
OliI	CACNNNNGTG	2 cut(s)	1293, 2285
PceI	AGGCCT	1 cut(s)	1160
PciI	ACATGT	1 cut(s)	2001
PciSI	GCTCTTC	1 cut(s)	1491
PctI	GAATGC	3 cut(s)	722, 812, 1945
PdiI	GCCGGC	1 cut(s)	2295
PfeI	GAWTC	6 cut(s)	125, 330, 667, 704, 1788, 1990
PflFI	GACNNNGTC	1 cut(s)	1070
PflMI	CCANNNNNTGG	2 cut(s)	305, 1599
PfoI	TCCNGGA	1 cut(s)	1974
PleI	GAGTC	3 cut(s)	757, 1311, 2126
PpsI	GAGTC	3 cut(s)	757, 1311, 2126
PscI	ACATGT	1 cut(s)	2001
PshAI	GACNNNNGTC	1 cut(s)	1290
PsiI	TTATAA	1 cut(s)	1476
Psp124BI	GAGCTC	2 cut(s)	111, 2343
Psp6I	CCWGG	5 cut(s)	882, 1092, 1209, 1974, 2143
PspEI	GGTNACC	1 cut(s)	1237
PspFI	CCCAGC	1 cut(s)	233
PspGI	CCWGG	5 cut(s)	882, 1092, 1209, 1974, 2143
PspN4I	GGNNCC	6 cut(s)	96, 435, 733, 874, 1406, 1741
PspOMI	GGGCCC	2 cut(s)	94, 433
PspPI	GGNCC	9 cut(s)	94, 95, 433, 434, 530, 865, 1405, 1739, 2141
PstNI	CAGNNNCTG	1 cut(s)	374
PsuI	RGATCY	5 cut(s)	26, 185, 697, 1616, 1837
PsyI	GACNNNGTC	1 cut(s)	1070
PvuII	CAGCTG	1 cut(s)	653
RsaI	GTAC	7 cut(s)	37, 874, 920, 1418, 1556, 2126, 2279
RsaNI	GTAC	7 cut(s)	36, 873, 919, 1417, 1555, 2125, 2278
RseI	CAYNNNNRTG	4 cut(s)	1293, 2000, 2045, 2285
SacI	GAGCTC	2 cut(s)	111, 2343
SapI	GCTCTTC	1 cut(s)	1491
SaqAI	TTAA	8 cut(s)	17, 53, 645, 894, 1101, 1143, 1341, 1485
Sau96I	GGNCC	9 cut(s)	94, 95, 433, 434, 530, 865, 1405, 1739, 2141
ScaI	AGTACT	1 cut(s)	1556
SchI	GAGTC	3 cut(s)	758, 1311, 2127
ScrFI	CCNGG	6 cut(s)	217, 884, 1094, 1211, 1976, 2145
SduI	GDGCHC	6 cut(s)	98, 111, 437, 684, 2265, 2343
SfaNI	GCATC	3 cut(s)	202, 1312, 2202
SinI	GGWCC	2 cut(s)	865, 1405
SmiMI	CAYNNNNRTG	4 cut(s)	1293, 2000, 2045, 2285
SmlI	CTYRAG	4 cut(s)	110, 644, 1841, 2264
SmoI	CTYRAG	4 cut(s)	110, 644, 1841, 2264
SseBI	AGGCCT	1 cut(s)	1160
SspMI	CTAG	3 cut(s)	828, 1314, 1502
SstI	GAGCTC	2 cut(s)	111, 2343
StuI	AGGCCT	1 cut(s)	1160
StyD4I	CCNGG	6 cut(s)	215, 882, 1092, 1209, 1974, 2143
StyI	CCWWGG	6 cut(s)	348, 564, 909, 1155, 1161, 1232
TaaI	ACNGT	7 cut(s)	514, 1292, 1649, 1678, 1967, 2129, 2258
TaqI	TCGA	8 cut(s)	333, 1089, 1320, 1621, 1791, 1872, 2043, 2070
TatI	WGTACW	3 cut(s)	1416, 1554, 2277
TauI	GCSGC	4 cut(s)	812, 1205, 1662, 2321
TfiI	GAWTC	6 cut(s)	125, 330, 667, 704, 1788, 1990
Tru1I	TTAA	8 cut(s)	17, 53, 645, 894, 1101, 1143, 1341, 1485
Tru9I	TTAA	8 cut(s)	17, 53, 645, 894, 1101, 1143, 1341, 1485
TscAI	CASTG	8 cut(s)	154, 540, 733, 850, 1864, 1947, 2226, 2353
TseFI	GTSAC	4 cut(s)	841, 1169, 1237, 1744
Tsp45I	GTSAC	4 cut(s)	841, 1169, 1237, 1744
TspDTI	ATGAA	6 cut(s)	17, 406, 651, 1511, 1736, 1842
TspGWI	ACGGA	2 cut(s)	857, 2099
TspRI	CASTG	8 cut(s)	154, 540, 733, 850, 1864, 1947, 2226, 2353
Tth111I	GACNNNGTC	1 cut(s)	1070
Van91I	CCANNNNNTGG	2 cut(s)	305, 1599
Vha464I	CTTAAG	1 cut(s)	644
VpaK11BI	GGWCC	2 cut(s)	865, 1405
XagI	CCTNNNNNAGG	1 cut(s)	1231
XapI	RAATTY	3 cut(s)	609, 902, 1470
XceI	RCATGY	2 cut(s)	1922, 2005
XcmI	CCANNNNNNNNNTGG	2 cut(s)	306, 691
XspI	CTAG	3 cut(s)	828, 1314, 1502
ZrmI	AGTACT	1 cut(s)	1556

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.