Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3B

Orthogroup ID

OG0004023

Physical Location & Seq

Genomic Coordinates Forward (+)

45092293 .. 45097300

Gene Structure 5008 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3BG365100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1761 bp

ATGATGAAATTCAGTGCCCTAAGAATTAGATCTATTAGTGCCAAGTTTTTGCATTTGAGAGAAATGGCGTCTCACATTGTTGGATACCCTCGTATGGGCCCCAAGAGAGAGCTCAAGTTTGCCTTGGAATCTTTCTGGGATGGCAAGAGCAGTGCCGAAGATTTGCAAAAGGTGTCTGCTGATCTCAGGTCTTCCATCTGGAAGCTGATGGCTGATACCGGGATCATGCACATTCCTAGCAACACTTTCTCTTACTACGACCAGGTGCTTGACACCACTGCAATGCTTGGGGCAGTTCCACCAAGATATGGTTGGAATGGTGGTGAGATTGGATTTGGCACGTATTTCTCCATGGCCAGAGGAAATGCCTCAGTTCCCGCTATGGAAATGACCAAGTGGTTCGATACCAACTACCATTTCATTGTCCCTGAGTTGGGTCCTGACGTTAAATTCTCATATGCTTCTCACAAGGCTGTTGAGGAATACAAGGAGGCTAAAGCGCTTGGAGTGGATACAATCCCAGTCCTTGTTGGTCCTGTGACCTACTTGTTGCTGTCCAAACCAGCAAAGGGTGTTGAAAAGACATTTTCGCTTCTCTCTCTTCTAGGAAAAATCATTCCTATTTACAAGGAAGTTATATCTGAGCTTAAGGCAGCTGGAGCTTCATGGATTCAGTTTGATGAGCCCACACTTGTTATGGATCTTGATTCTCACAAGTTGCAAGCATTCACTGATGCTTACTCTCAGCTAGAGTCAACTCTGTCCGGCTTGAATGTTCTTATTGAGACCTACTTTGCAGATGTACCTGCAGAGGCATACAAGACTCTCACTTCCTTGAAGGGTGTCACTGCCTATGGTTTTGATTTAGTTCGTGGTTCTAAGACCCTTGAGTTAATTAAGAGCGACTTCCCTAAAGGAAAGTGCCTCTTTGCTGGATTGGTTGATGGAAGGAACATTTGGGCTAATGATCTCTCTGCTTCCCTAAGTACATTGCAGGCTCTTGAAGGCATTGTTGGCAAAGACCACCTTGTCGTCTCAACCTCCTGCTCTTGTCTTCACACTGCTGTTGATCTTGTTAATGAGACTAAGCTGGACAATGAAATCAAATCGTGGCTCGCCTTTGCTGCACAGAAAATTCTTGAAGTTAATGCTTTGGCAAAGGCATTGGCTGGTCACAAGGATGAGTCATTTTTCTCAAGCAACGCTGCAGCTCTGGCTTCAAGGAAATCGTCCCCAAGGGTGACCAATGAGGCTGTTCAAAAGGCTGCTGCTGCTTTGAAGGGTTCTGACCACCGTCGTGCTACTAATGTAAGTGCCAGACTTGATGCTCAGCAGAAGAAGCTTAATCTTCCAGTTCTCCCAACCACCACCATCGGATCATTCCCTCAGACCATCGAACTGAGAAGGGTTCGCCGTGAATACAAGGCCAAAAAGATCTCTGAGGAGGAGTATGTCAAGGCCATTAAGGAGGAAATTAGCAAAGTTGTCAAGCTTCAGGAAGAGCTTGACATTGATGTCCTTGTTCATGGGGAGCCTGAGGTGAGCTTTCATTTATTAAATTCTGTCAGGAAGGATGAAGAGAAACCTGTTCTACTATTGGATGGTGAATTTTGGCTTTTCATTCTGTATGTGAGACTGCAATTCATTATTGTTTATTCTCTTGGCAGAGGAACGACATGGTTGAGTACTTTGGGGAGCAATTATCAGGGTTTGCCTTTACTGTCAATGGGTGGGTGCAATCTTATGGATCTCGATGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000096 GO:0000097 GO:0003674 GO:0003824 GO:0003871 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005777 GO:0005829 GO:0005886 GO:0005911 GO:0006082 GO:0006520 GO:0006555 GO:0006790 GO:0006807 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0008168 GO:0008172 GO:0008270 GO:0008652 GO:0008705 GO:0009058 GO:0009066 GO:0009067 GO:0009086 GO:0009506 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009651 GO:0009941 GO:0009987 GO:0010035 GO:0010038 GO:0010043 GO:0016020 GO:0016053 GO:0016740 GO:0016741 GO:0019752 GO:0030054 GO:0031090 GO:0031967 GO:0031975 GO:0032259 GO:0042084 GO:0042085 GO:0042221 GO:0042579 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044272 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046686 GO:0046872 GO:0046914 GO:0048046 GO:0050667 GO:0050896 GO:0055044 GO:0071704 GO:0071944 GO:0098588 GO:0098805 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00270 ko00450 ko01100 ko01110 ko01230 map00270 map00450 map01100 map01110 map01230

Pfam Domains

Protein Families

Meth_synt_1 Meth_synt_2

Protein Analysis

586

Amino Acids

64.84

Weight (kDa)

6.88

Isoelectric Point (pI)

35.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Meth_synt_1	PF08267	24 - 336	9.4e-116	Cobalamin-independent synthase, N-terminal domain
Meth_synt_2	PF01717	453 - 513	1.4e-20	Cobalamin-independent synthase, Catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0004023)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G03780 AT3G03780 AT3G03780 AT5G17920 AT5G17920
fragaria_vesca	FvH4_1g28390 FvH4_1g28390 FvH4_1g28390 FvH4_1g28390 FvH4_2g41260 FvH4_2g41260 FvH4_2g41260 FvH4_2g41260 FvH4_2g41260 FvH4_2g41260
malus_domestica	MD12G1008100.v1.1 MD14G1005500.v1.1
prunus_persica	Prupe.7G009200_v2.0.a1 Prupe.7G009200_v2.0.a1 Prupe.7G009200_v2.0.a1
pyrus_communis	pycom12g00610 pycom14g00410
rosa_chinensis	RchiOBHm_Chr3g0496511
rosa_laevigata	RLG00000022522
rosa_multiflora	Rmu_sc0000620.1_g000008
rosa_roxburghii	Rroxscaffold_6G00389060
rosa_rugosa	Rorug03G0283400 Rorug03G0283500
rosa_samantha	Rh3AG329600 Rh3BG365100 Rh3CG362500 Rh3DG365600
rosa_wichuraiana	Rw3G028830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	1028, 1514
Acc36I	ACCTGC	1 cut(s)	814
AccB7I	CCANNNNNTGG	1 cut(s)	308
AciI	CCGC	1 cut(s)	378
AclWI	GGATC	4 cut(s)	230, 708, 1384, 1755
AcoI	YGGCCR	1 cut(s)	354
AcsI	RAATTY	5 cut(s)	8, 449, 1134, 1558, 1607
AcuI	CTGAAG	1 cut(s)	1478
AcyI	GRCGYC	1 cut(s)	68
AfaI	GTAC	3 cut(s)	804, 988, 1687
AfeI	AGCGCT	1 cut(s)	501
AfiI	CCNNNNNNNGG	6 cut(s)	94, 95, 308, 433, 434, 569
AflII	CTTAAG	1 cut(s)	647
AgsI	TTSAA	8 cut(s)	578, 772, 838, 1004, 1142, 1221, 1259, 1279
AjnI	CCWGG	1 cut(s)	261
AjuI	GAANNNNNNNTTGG	6 cut(s)	401, 433, 940, 972, 996, 1028
AleI	CACNNNNGTG	1 cut(s)	1296
Alw21I	GWGCWC	1 cut(s)	114
Alw26I	GTCTC	5 cut(s)	75, 779, 1039, 1076, 1627
AlwI	GGATC	4 cut(s)	230, 708, 1384, 1755
Aor51HI	AGCGCT	1 cut(s)	501
AoxI	GGCC	4 cut(s)	97, 354, 1425, 1458
ApaI	GGGCCC	1 cut(s)	101
ApeKI	GCWGC	7 cut(s)	653, 1124, 1205, 1208, 1265, 1268, 1271
ApoI	RAATTY	5 cut(s)	8, 449, 1134, 1558, 1607
AspLEI	GCGC	1 cut(s)	502
AspS9I	GGNCC	4 cut(s)	97, 98, 437, 533
AsuC2I	CCSGG	1 cut(s)	220
AsuHPI	GGTGA	4 cut(s)	335, 1252, 1552, 1616
AvaII	GGWCC	2 cut(s)	437, 533
AxyI	CCTNAGG	1 cut(s)	1536
BaeGI	GKGCMC	2 cut(s)	19, 101
BaeI	ACNNNNGTAYC	2 cut(s)	76, 109
BalI	TGGCCA	1 cut(s)	356
BanII	GRGCYC	3 cut(s)	101, 114, 687
BbsI	GAAGAC	2 cut(s)	183, 1046
Bbv12I	GWGCWC	1 cut(s)	114
BbvI	GCAGC	7 cut(s)	665, 1111, 1192, 1220, 1252, 1255, 1258
BccI	CCATC	7 cut(s)	134, 202, 203, 938, 1379, 1400, 1595
BceAI	ACGGC	1 cut(s)	1398
BciT130I	CCWGG	1 cut(s)	263
BciVI	GTATCC	2 cut(s)	77, 505
BcnI	CCSGG	1 cut(s)	220
BcoDI	GTCTC	5 cut(s)	75, 779, 1039, 1076, 1627
BfaI	CTAG	3 cut(s)	237, 605, 749
BfmI	CTRYAG	2 cut(s)	807, 1206
BfoI	RGCGCY	1 cut(s)	503
BfrI	CTTAAG	1 cut(s)	647
BfuAI	ACCTGC	1 cut(s)	814
BfuI	GTATCC	2 cut(s)	77, 505
BglII	AGATCT	2 cut(s)	29, 1434
BisI	GCNGC	7 cut(s)	654, 1125, 1206, 1209, 1266, 1269, 1272
BlpI	GCTNAGC	1 cut(s)	1329
BlsI	GCNGC	7 cut(s)	655, 1126, 1207, 1210, 1267, 1270, 1273
BmcAI	AGTACT	1 cut(s)	1687
Bme1390I	CCNGG	2 cut(s)	220, 263
Bme18I	GGWCC	2 cut(s)	437, 533
BmgT120I	GGNCC	4 cut(s)	97, 98, 437, 533
BmiI	GGNNCC	4 cut(s)	99, 100, 438, 1533
BmrFI	CCNGG	2 cut(s)	220, 263
BmrI	ACTGGG	1 cut(s)	515
BmsI	GCATC	2 cut(s)	724, 1315
BmuI	ACTGGG	1 cut(s)	515
BoxI	GACNNNNGTC	1 cut(s)	1293
BpiI	GAAGAC	2 cut(s)	183, 1046
BpmI	CTGGAG	1 cut(s)	678
Bpu1102I	GCTNAGC	1 cut(s)	1329
BpuEI	CTTGAG	3 cut(s)	98, 908, 1180
BpuMI	CCSGG	1 cut(s)	220
BsaAI	YACGTR	1 cut(s)	342
BsaHI	GRCGYC	1 cut(s)	68
BsaI	GGTCTC	1 cut(s)	779
BsaJI	CCNNGG	3 cut(s)	123, 351, 1235
Bsc4I	CCNNNNNNNGG	6 cut(s)	94, 95, 308, 433, 434, 569
Bse1I	ACTGG	2 cut(s)	521, 1352
Bse21I	CCTNAGG	1 cut(s)	1536
Bse3DI	GCAATG	2 cut(s)	288, 989
BseBI	CCWGG	1 cut(s)	263
BseDI	CCNNGG	3 cut(s)	123, 351, 1235
BseGI	GGATG	4 cut(s)	145, 1186, 1579, 1606
BseLI	CCNNNNNNNGG	6 cut(s)	94, 95, 308, 433, 434, 569
BseMI	GCAATG	2 cut(s)	288, 989
BseNI	ACTGG	2 cut(s)	521, 1352
BseRI	GAGGAG	2 cut(s)	1457, 1460
BseSI	GKGCMC	2 cut(s)	19, 101
BseXI	GCAGC	7 cut(s)	665, 1111, 1192, 1220, 1252, 1255, 1258
BsgI	GTGCAG	1 cut(s)	1110
BshFI	GGCC	4 cut(s)	99, 356, 1427, 1460
BsiHKAI	GWGCWC	1 cut(s)	114
BsiSI	CCGG	2 cut(s)	219, 765
BslFI	GGGAC	2 cut(s)	410, 1216
BslI	CCNNNNNNNGG	6 cut(s)	94, 95, 308, 433, 434, 569
BsmAI	GTCTC	5 cut(s)	75, 779, 1039, 1076, 1627
BsmBI	CGTCTC	2 cut(s)	75, 1039
BsmFI	GGGAC	2 cut(s)	410, 1216
BsmI	GAATGC	1 cut(s)	725
BsnI	GGCC	4 cut(s)	99, 356, 1427, 1460
Bso31I	GGTCTC	1 cut(s)	779
Bsp120I	GGGCCC	1 cut(s)	97
Bsp1286I	GDGCHC	4 cut(s)	19, 101, 114, 687
Bsp143I	GATC	9 cut(s)	29, 181, 222, 700, 967, 1069, 1376, 1434, 1747
Bsp1720I	GCTNAGC	1 cut(s)	1329
Bsp19I	CCATGG	1 cut(s)	351
BspACI	CCGC	1 cut(s)	378
BspANI	GGCC	4 cut(s)	99, 356, 1427, 1460
BspLI	GGNNCC	4 cut(s)	99, 100, 438, 1533
BspMAI	CTGCAG	2 cut(s)	811, 1210
BspMI	ACCTGC	1 cut(s)	814
BspPI	GGATC	4 cut(s)	230, 708, 1384, 1755
BspQI	GCTCTTC	1 cut(s)	1494
BspTI	CTTAAG	1 cut(s)	647
BspTNI	GGTCTC	1 cut(s)	779
BsrDI	GCAATG	2 cut(s)	288, 989
BsrI	ACTGG	2 cut(s)	521, 1352
BssECI	CCNNGG	3 cut(s)	123, 351, 1235
BssMI	GATC	9 cut(s)	29, 181, 222, 700, 967, 1069, 1376, 1434, 1747
BssNI	GRCGYC	1 cut(s)	68
BssT1I	CCWWGG	3 cut(s)	123, 351, 1235
Bst2UI	CCWGG	1 cut(s)	263
Bst4CI	ACNGT	2 cut(s)	1295, 1722
Bst6I	CTCTTC	3 cut(s)	606, 1494, 1572
BstACI	GRCGYC	1 cut(s)	68
BstAFI	CTTAAG	1 cut(s)	647
BstBAI	YACGTR	1 cut(s)	342
BstC8I	GCNNGC	3 cut(s)	723, 996, 1116
BstDSI	CCRYGG	1 cut(s)	351
BstEII	GGTNACC	1 cut(s)	1240
BstF5I	GGATG	4 cut(s)	145, 1186, 1579, 1606
BstH2I	RGCGCY	1 cut(s)	503
BstHHI	GCGC	1 cut(s)	502
BstKTI	GATC	9 cut(s)	32, 184, 225, 703, 970, 1072, 1379, 1437, 1750
BstMAI	GTCTC	5 cut(s)	75, 779, 1039, 1076, 1627
BstMBI	GATC	9 cut(s)	29, 181, 222, 700, 967, 1069, 1376, 1434, 1747
BstMWI	GCNNNNNNNGC	6 cut(s)	659, 1014, 1124, 1214, 1271, 1339
BstNI	CCWGG	1 cut(s)	263
BstPAI	GACNNNNGTC	1 cut(s)	1293
BstPI	GGTNACC	1 cut(s)	1240
BstSCI	CCNGG	2 cut(s)	218, 261
BstSFI	CTRYAG	2 cut(s)	807, 1206
BstSLI	GKGCMC	2 cut(s)	19, 101
BstV1I	GCAGC	7 cut(s)	665, 1111, 1192, 1220, 1252, 1255, 1258
BstV2I	GAAGAC	2 cut(s)	183, 1046
BstX2I	RGATCY	4 cut(s)	29, 700, 1434, 1747
BstYI	RGATCY	4 cut(s)	29, 700, 1434, 1747
Bsu36I	CCTNAGG	1 cut(s)	1536
BsuI	GTATCC	2 cut(s)	77, 505
BsuRI	GGCC	4 cut(s)	99, 356, 1427, 1460
BtgI	CCRYGG	1 cut(s)	351
BtsCI	GGATG	4 cut(s)	145, 1186, 1579, 1606
BtsI	GCAGTG	4 cut(s)	157, 276, 846, 1059
BtsIMutI	CAGTG	6 cut(s)	19, 157, 276, 729, 846, 1059
BveI	ACCTGC	1 cut(s)	814
Cac8I	GCNNGC	3 cut(s)	723, 996, 1116
CfoI	GCGC	1 cut(s)	502
Cfr13I	GGNCC	4 cut(s)	97, 98, 437, 533
CseI	GACGC	1 cut(s)	57
CsiI	ACCWGGT	1 cut(s)	261
Csp6I	GTAC	3 cut(s)	803, 987, 1686
CviAII	CATG	5 cut(s)	226, 352, 666, 1526, 1677
CviQI	GTAC	3 cut(s)	803, 987, 1686
DpnI	GATC	9 cut(s)	31, 183, 224, 702, 969, 1071, 1378, 1436, 1749
DpnII	GATC	9 cut(s)	29, 181, 222, 700, 967, 1069, 1376, 1434, 1747
DrdI	GACNNNNNNGTC	2 cut(s)	1028, 1514
DseDI	GACNNNNNNGTC	2 cut(s)	1028, 1514
EaeI	YGGCCR	1 cut(s)	354
Eam1104I	CTCTTC	3 cut(s)	606, 1494, 1572
EarI	CTCTTC	3 cut(s)	606, 1494, 1572
Ecl136II	GAGCTC	1 cut(s)	112
Eco130I	CCWWGG	3 cut(s)	123, 351, 1235
Eco24I	GRGCYC	3 cut(s)	101, 114, 687
Eco31I	GGTCTC	1 cut(s)	779
Eco47I	GGWCC	2 cut(s)	437, 533
Eco47III	AGCGCT	1 cut(s)	501
Eco53kI	GAGCTC	1 cut(s)	112
Eco57I	CTGAAG	1 cut(s)	1478
Eco81I	CCTNAGG	1 cut(s)	1536
Eco91I	GGTNACC	1 cut(s)	1240
EcoICRI	GAGCTC	1 cut(s)	112
EcoO109I	RGGNCCY	2 cut(s)	98, 437
EcoO65I	GGTNACC	1 cut(s)	1240
EcoRII	CCWGG	1 cut(s)	261
EcoT14I	CCWWGG	3 cut(s)	123, 351, 1235
EcoT38I	GRGCYC	3 cut(s)	101, 114, 687
ErhI	CCWWGG	3 cut(s)	123, 351, 1235
Esp3I	CGTCTC	2 cut(s)	75, 1039
FaeI	CATG	5 cut(s)	229, 355, 669, 1529, 1680
FalI	AAGNNNNNCTT	8 cut(s)	107, 139, 890, 922, 911, 943, 1011, 1043
FaqI	GGGAC	2 cut(s)	410, 1216
FatI	CATG	5 cut(s)	225, 351, 665, 1525, 1676
FauI	CCCGC	1 cut(s)	385
FauNDI	CATATG	1 cut(s)	457
Fnu4HI	GCNGC	7 cut(s)	654, 1125, 1206, 1209, 1266, 1269, 1272
FokI	GGATG	4 cut(s)	152, 1193, 1586, 1613
FriOI	GRGCYC	3 cut(s)	101, 114, 687
Fsp4HI	GCNGC	7 cut(s)	654, 1125, 1206, 1209, 1266, 1269, 1272
FspBI	CTAG	3 cut(s)	237, 605, 749
GlaI	GCGC	1 cut(s)	501
GluI	GCNGC	7 cut(s)	654, 1125, 1206, 1209, 1266, 1269, 1272
GsuI	CTGGAG	1 cut(s)	678
HaeII	RGCGCY	1 cut(s)	503
HaeIII	GGCC	4 cut(s)	99, 356, 1427, 1460
HapII	CCGG	2 cut(s)	219, 765
HgaI	GACGC	1 cut(s)	57
HhaI	GCGC	1 cut(s)	502
Hin1I	GRCGYC	1 cut(s)	68
Hin1II	CATG	5 cut(s)	229, 355, 669, 1529, 1680
Hin6I	GCGC	1 cut(s)	500
HinP1I	GCGC	1 cut(s)	500
HincII	GTYRAC	1 cut(s)	756
HindII	GTYRAC	1 cut(s)	756
HindIII	AAGCTT	2 cut(s)	1340, 1490
HinfI	GANTC	6 cut(s)	128, 670, 707, 752, 823, 1184
HpaII	CCGG	2 cut(s)	219, 765
HphI	GGTGA	4 cut(s)	335, 1252, 1552, 1616
Hpy166II	GTNNAC	1 cut(s)	756
Hpy188I	TCNGA	5 cut(s)	643, 1288, 1376, 1389, 1441
Hpy188III	TCNNGA	8 cut(s)	199, 440, 704, 1001, 1139, 1496, 1567, 1751
Hpy8I	GTNNAC	1 cut(s)	756
Hpy99I	CGWCG	1 cut(s)	1299
HpyAV	CCTTC	6 cut(s)	832, 942, 998, 1273, 1398, 1564
HpyCH4III	ACNGT	2 cut(s)	1295, 1722
HpyCH4IV	ACGT	2 cut(s)	341, 444
HpyF10VI	GCNNNNNNNGC	6 cut(s)	659, 1014, 1124, 1214, 1271, 1339
HpySE526I	ACGT	2 cut(s)	341, 444
Hsp92I	GRCGYC	1 cut(s)	68
Hsp92II	CATG	5 cut(s)	229, 355, 669, 1529, 1680
HspAI	GCGC	1 cut(s)	500
Kzo9I	GATC	9 cut(s)	29, 181, 222, 700, 967, 1069, 1376, 1434, 1747
LguI	GCTCTTC	1 cut(s)	1494
LmnI	GCTCC	3 cut(s)	659, 1531, 1695
Lsp1109I	GCAGC	7 cut(s)	665, 1111, 1192, 1220, 1252, 1255, 1258
LweI	GCATC	2 cut(s)	724, 1315
MabI	ACCWGGT	1 cut(s)	261
MaeI	CTAG	3 cut(s)	237, 605, 749
MaeII	ACGT	2 cut(s)	341, 444
MaeIII	GTNAC	4 cut(s)	538, 844, 1172, 1240
MalI	GATC	9 cut(s)	31, 183, 224, 702, 969, 1071, 1378, 1436, 1749
MboI	GATC	9 cut(s)	29, 181, 222, 700, 967, 1069, 1376, 1434, 1747
MboII	GAAGA	8 cut(s)	170, 183, 593, 1046, 1340, 1348, 1511, 1589
MflI	RGATCY	4 cut(s)	29, 700, 1434, 1747
MhlI	GDGCHC	4 cut(s)	19, 101, 114, 687
MlsI	TGGCCA	1 cut(s)	356
MluNI	TGGCCA	1 cut(s)	356
MlyI	GAGTC	3 cut(s)	761, 817, 1193
MmeI	TCCRAC	2 cut(s)	61, 293
Mox20I	TGGCCA	1 cut(s)	356
MscI	TGGCCA	1 cut(s)	356
MseI	TTAA	9 cut(s)	447, 648, 893, 897, 1077, 1146, 1344, 1464, 1556
MslI	CAYNNNNRTG	3 cut(s)	281, 1179, 1296
Msp20I	TGGCCA	1 cut(s)	356
MspA1I	CMGCKG	1 cut(s)	656
MspCI	CTTAAG	1 cut(s)	647
MspI	CCGG	2 cut(s)	219, 765
MspR9I	CCNGG	2 cut(s)	220, 263
Mva1269I	GAATGC	1 cut(s)	725
MvaI	CCWGG	1 cut(s)	263
MwoI	GCNNNNNNNGC	6 cut(s)	659, 1014, 1124, 1214, 1271, 1339
NciI	CCSGG	1 cut(s)	220
NcoI	CCATGG	1 cut(s)	351
NdeI	CATATG	1 cut(s)	457
NdeII	GATC	9 cut(s)	29, 181, 222, 700, 967, 1069, 1376, 1434, 1747
NlaIII	CATG	5 cut(s)	229, 355, 669, 1529, 1680
NlaIV	GGNNCC	4 cut(s)	99, 100, 438, 1533
NmuCI	GTSAC	4 cut(s)	538, 844, 1172, 1240
OliI	CACNNNNGTG	1 cut(s)	1296
PacI	TTAATTAA	1 cut(s)	897
PciSI	GCTCTTC	1 cut(s)	1494
PctI	GAATGC	1 cut(s)	725
PfeI	GAWTC	3 cut(s)	128, 670, 707
PflMI	CCANNNNNTGG	1 cut(s)	308
PkrI	GCNGC	7 cut(s)	655, 1126, 1207, 1210, 1267, 1270, 1273
PleI	GAGTC	3 cut(s)	760, 817, 1192
PpsI	GAGTC	3 cut(s)	760, 817, 1192
Ppu21I	YACGTR	1 cut(s)	342
PpuMI	RGGWCCY	1 cut(s)	437
PshAI	GACNNNNGTC	1 cut(s)	1293
Psp124BI	GAGCTC	1 cut(s)	114
Psp5II	RGGWCCY	1 cut(s)	437
Psp6I	CCWGG	1 cut(s)	261
PspEI	GGTNACC	1 cut(s)	1240
PspGI	CCWGG	1 cut(s)	261
PspN4I	GGNNCC	4 cut(s)	99, 100, 438, 1533
PspOMI	GGGCCC	1 cut(s)	97
PspPI	GGNCC	4 cut(s)	97, 98, 437, 533
PspPPI	RGGWCCY	1 cut(s)	437
PstI	CTGCAG	2 cut(s)	811, 1210
PsuI	RGATCY	4 cut(s)	29, 700, 1434, 1747
PvuII	CAGCTG	1 cut(s)	656
RsaI	GTAC	3 cut(s)	804, 988, 1687
RsaNI	GTAC	3 cut(s)	803, 987, 1686
RseI	CAYNNNNRTG	3 cut(s)	281, 1179, 1296
SacI	GAGCTC	1 cut(s)	114
SapI	GCTCTTC	1 cut(s)	1494
SaqAI	TTAA	9 cut(s)	447, 648, 893, 897, 1077, 1146, 1344, 1464, 1556
SatI	GCNGC	7 cut(s)	654, 1125, 1206, 1209, 1266, 1269, 1272
Sau3AI	GATC	9 cut(s)	29, 181, 222, 700, 967, 1069, 1376, 1434, 1747
Sau96I	GGNCC	4 cut(s)	97, 98, 437, 533
ScaI	AGTACT	1 cut(s)	1687
SchI	GAGTC	3 cut(s)	761, 817, 1193
ScrFI	CCNGG	2 cut(s)	220, 263
SduI	GDGCHC	4 cut(s)	19, 101, 114, 687
SexAI	ACCWGGT	1 cut(s)	261
SfaNI	GCATC	2 cut(s)	724, 1315
SfcI	CTRYAG	2 cut(s)	807, 1206
SinI	GGWCC	2 cut(s)	437, 533
SmiMI	CAYNNNNRTG	3 cut(s)	281, 1179, 1296
SmlI	CTYRAG	4 cut(s)	113, 647, 887, 1195
SmoI	CTYRAG	4 cut(s)	113, 647, 887, 1195
SsiI	CCGC	1 cut(s)	378
SspMI	CTAG	3 cut(s)	237, 605, 749
SstI	GAGCTC	1 cut(s)	114
StyD4I	CCNGG	2 cut(s)	218, 261
StyI	CCWWGG	3 cut(s)	123, 351, 1235
TaaI	ACNGT	2 cut(s)	1295, 1722
TaiI	ACGT	2 cut(s)	344, 447
TaqI	TCGA	3 cut(s)	402, 1395, 1752
TatI	WGTACW	2 cut(s)	986, 1685
TfiI	GAWTC	3 cut(s)	128, 670, 707
Tru1I	TTAA	9 cut(s)	447, 648, 893, 897, 1077, 1146, 1344, 1464, 1556
Tru9I	TTAA	9 cut(s)	447, 648, 893, 897, 1077, 1146, 1344, 1464, 1556
TscAI	CASTG	6 cut(s)	19, 157, 283, 736, 853, 1066
TseFI	GTSAC	4 cut(s)	538, 844, 1172, 1240
TseI	GCWGC	7 cut(s)	653, 1124, 1205, 1208, 1265, 1268, 1271
Tsp45I	GTSAC	4 cut(s)	538, 844, 1172, 1240
TspDTI	ATGAA	9 cut(s)	20, 409, 654, 1113, 1514, 1538, 1590, 1609, 1633
TspRI	CASTG	6 cut(s)	19, 157, 283, 736, 853, 1066
Van91I	CCANNNNNTGG	1 cut(s)	308
Vha464I	CTTAAG	1 cut(s)	647
VpaK11BI	GGWCC	2 cut(s)	437, 533
XapI	RAATTY	5 cut(s)	8, 449, 1134, 1558, 1607
XcmI	CCANNNNNNNNNTGG	2 cut(s)	309, 694
XspI	CTAG	3 cut(s)	237, 605, 749
ZrmI	AGTACT	1 cut(s)	1687

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.