Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0004023

Physical Location & Seq

Genomic Coordinates Reverse (-)

865556 .. 870716

Gene Structure 5161 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1008100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2268 bp

ATGGGCCCTAAGAGAGAGCTCAAGTTTGCTCTTGAATCTTTCTGGGATGGCAAGAGCAGTGCTGAGGACTTGCAGAAGGTGTCTGCTGATCTCAGATCTTCCATCTGGAAGCAGATGGCTGATGCCGGGATCAAGCACATTCCCAGCAACACCTTCTCATACTACGATCAGGTGCTTGACACCACCGCATTGCTTGGGGCTGTCCCACCAAGATATGGGTGGAACGGCGGTGAGATTGGATTCGATACATACTTCTCCATGGCCAGAGGAAATGCCTCAGTTCCTGCTATGGAAATGACCAAGTGGTTTGATACCAACTACCATTTCATTGTCCCTGAGTTGGGCCCTGATGTGAACTTCTCTTATGCTTCTCACAAGGCTGTTGAAGAATACAAGGAGGCTAAGGCGCTTGGAGTGGATACTGTCCCAGTCCTTGTTGGCCCAGTGTCATACTTGTTGCTGTCCAAGCCAGCCAAGGGTGTTGAGAAGACCTTTTCTCTTCTCTCTCTTCTTCCTAAAATTCTTCCTATCTATAAGGAAGTTGTATCTGAGCTTAAGGCAGCTGGAGCTTCATGGATTCAATTTGATGAGCCCACACTTGTTTTGGATCTTGAATCCGACAAGTTGCAAGCATTCACTGGTGCCTACTCTGAACTGGAGTCAGCTTTATCTGGCTTGAATGTTCTTATTGAGACCTACTTTGCTGATGTTCCTGCTGCGGCATTCAAGACCCTCACTAGCTTGAAGGGTGTCACCGCTTATGGTTTTGATTTGGTCCGTGGTACCAAGACCCTGGATTTGATTAAGAGCAAATTCCCCAAGGGAAAGTACCTCTTTGCCGGTGTGGTTGATGGAAGGAACATCTGGGCTAATGATCTTTGTGCCTCTCTAAGCACATTGGAGACCCTTGAAGGCATTGTTGGAAAAGATAAACTTGTTGTCTCCACCTCCACCTCTCTTCTTCACACTGCTGTTGACCTTGTCAATGAGACCAAGCTCGACCAGGAAATTAAGTCATGGTTGGCTTTTGCTGCCCAGAAAATTATTGAAGTTAATGCTTTGGCCAAGGCCTTGGCTGGTCACAAGGATGAGGCATTTTTCTCTGCTAATGCCGCTGCCCAGGCTTCAAGGAAGTCCTCCCCAAGGGTGACCAATGAGGCTGTTCAGAAGGCTGCTGCTGCTTTGAAGGATTCTGACCACCGTCGTGCTACCAATGTTAGTGCTAGACTCGATGCTCAACAGAAGAAGCTTAACCTTCCAATTCTCCCAACCACCACCATTGGATCGTTCCCTCAGACCATTGAACTCAGGAGGGTCCGCCGTGAGTACAAGGCCAAGAAGATTTCTGAGGAGGAATATGTCAAGGCCATCAAGGAAGAAATTAAGAAGGTTGTTAAACTTCAGGAAGAGCTAGACATTGATGTCCTTGTTCATGGAGAGCCTGAGAGGAACGATATGGTTGAGTACTTCGGAGAGCAATTGTCTGGCTTTGCCTTTACCGCCAATGGGTGGGTGCAATCATATGGATCTCGATGTGTGAAGCCACCAATCATCTACGGTGATGTGAGCCGCCCCAAGCCTATGACTGTATTCTGGTCATGTGCTGCTCAGAGCATGACTGCCCGCCCAATGAAGGGTATGCTTACCGGCCCCGTCACCATCCTGAACTGGTCGTTCGTCAGAAATGACCAACCCAGATTCGAGACCACCTTTCAGATTGCTTTGGCCATCAAGGATGAAGTGGAGGATCTTGAGAAGGCTGGTATCAGTGTTATTCAGATCGATGAGGCTGCTTTGAGAGAGGGATTGCCTCTAAGGAAGTCTGAACATGCTTTCTACTTGGATTGGGCTGTGCACTCCTTCAGGATCACCAACAGTGGTGTCCAGGACACTACCCAGATTCACACTCACATGTGCTACTCCAACTTCAACGACATCATCCACTCAATCATCGACATGGACGCTGATGTGATCACCATTGAGAACTCTCGTTCCGATGAGAAGCTCCTGTCAGTCTTCCGTGAGGGAGTCAAGTACGGTGCTGGAATCGGCCCTGGTGTGTATGACATCCACTCTCCAAGAATACCATCAACCGACGAGATTGCTGACCGGATCAACAAGATGCTTGCAGTGCTGGAGACCAACATCTTGTGGGTGAACCCCGACTGTGGGCTCAAGACCCGCAAGTACCCAGAAGTGAAGCCGGCGCTCAGCAACATGGTTGCTGCCGCCAAGCTCCTCCGCACCCAGCTCGCCAGTGCCAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000096 GO:0000097 GO:0003674 GO:0003824 GO:0003871 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005777 GO:0005829 GO:0005886 GO:0005911 GO:0006082 GO:0006520 GO:0006555 GO:0006790 GO:0006807 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0008168 GO:0008172 GO:0008270 GO:0008652 GO:0008705 GO:0009058 GO:0009066 GO:0009067 GO:0009086 GO:0009506 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009651 GO:0009941 GO:0009987 GO:0010035 GO:0010038 GO:0010043 GO:0016020 GO:0016053 GO:0016740 GO:0016741 GO:0019752 GO:0030054 GO:0031090 GO:0031967 GO:0031975 GO:0032259 GO:0042084 GO:0042085 GO:0042221 GO:0042579 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044272 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046686 GO:0046872 GO:0046914 GO:0048046 GO:0050667 GO:0050896 GO:0055044 GO:0071704 GO:0071944 GO:0098588 GO:0098805 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00270 ko00450 ko01100 ko01110 ko01230 map00270 map00450 map01100 map01110 map01230

Pfam Domains

Protein Families

Meth_synt_1 Meth_synt_2

Protein Analysis

756

Amino Acids

83.5

Weight (kDa)

6.19

Isoelectric Point (pI)

33.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Meth_synt_1	PF08267	1 - 305	3.7e-112	Cobalamin-independent synthase, N-terminal domain
Meth_synt_2	PF01717	422 - 745	1.5e-159	Cobalamin-independent synthase, Catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0004023)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G03780 AT3G03780 AT3G03780 AT5G17920 AT5G17920
fragaria_vesca	FvH4_1g28390 FvH4_1g28390 FvH4_1g28390 FvH4_1g28390 FvH4_2g41260 FvH4_2g41260 FvH4_2g41260 FvH4_2g41260 FvH4_2g41260 FvH4_2g41260
malus_domestica	MD12G1008100.v1.1 MD14G1005500.v1.1
prunus_persica	Prupe.7G009200_v2.0.a1 Prupe.7G009200_v2.0.a1 Prupe.7G009200_v2.0.a1
pyrus_communis	pycom12g00610 pycom14g00410
rosa_chinensis	RchiOBHm_Chr3g0496511
rosa_laevigata	RLG00000022522
rosa_multiflora	Rmu_sc0000620.1_g000008
rosa_roxburghii	Rroxscaffold_6G00389060
rosa_rugosa	Rorug03G0283400 Rorug03G0283500
rosa_samantha	Rh3AG329600 Rh3BG365100 Rh3CG362500 Rh3DG365600
rosa_wichuraiana	Rw3G028830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1421
Acc65I	GGTACC	1 cut(s)	782
AccB1I	GGYRCC	2 cut(s)	641, 782
AccB7I	CCANNNNNTGG	2 cut(s)	215, 1509
AclWI	GGATC	7 cut(s)	137, 615, 1291, 1534, 1755, 1874, 2120
AcoI	YGGCCR	3 cut(s)	261, 1062, 1725
AcsI	RAATTY	2 cut(s)	519, 812
AcuI	CTGAAG	2 cut(s)	1385, 1846
AfaI	GTAC	6 cut(s)	784, 830, 1328, 1466, 2036, 2189
AflII	CTTAAG	1 cut(s)	554
AflIII	ACRYGT	1 cut(s)	1911
AjnI	CCWGG	5 cut(s)	792, 1002, 1119, 1884, 2053
AjuI	GAANNNNNNNTTGG	4 cut(s)	308, 340, 903, 935
AleI	CACNNNNGTG	1 cut(s)	1203
AloI	GAACNNNNNNTCC	2 cut(s)	1976, 2008
Alw21I	GWGCWC	2 cut(s)	21, 1857
Alw26I	GTCTC	6 cut(s)	686, 896, 946, 983, 1697, 2132
Alw44I	GTGCAC	1 cut(s)	1853
AlwI	GGATC	7 cut(s)	137, 615, 1291, 1534, 1755, 1874, 2120
AlwNI	CAGNNNCTG	1 cut(s)	284
ApaI	GGGCCC	2 cut(s)	8, 347
ApaLI	GTGCAC	1 cut(s)	1853
ApoI	RAATTY	2 cut(s)	519, 812
ArsI	GACNNNNNNTTYG	2 cut(s)	781, 813
Asp718I	GGTACC	1 cut(s)	782
AspLEI	GCGC	2 cut(s)	409, 2209
AspS9I	GGNCC	9 cut(s)	4, 5, 343, 344, 440, 775, 1315, 1649, 2051
AsuC2I	CCSGG	1 cut(s)	127
AsuHPI	GGTGA	8 cut(s)	242, 745, 1159, 1571, 1648, 1861, 1966, 2167
AvaII	GGWCC	2 cut(s)	775, 1315
BaeGI	GKGCMC	3 cut(s)	8, 347, 1857
BalI	TGGCCA	3 cut(s)	263, 1064, 1727
BanI	GGYRCC	2 cut(s)	641, 782
BanII	GRGCYC	5 cut(s)	8, 21, 347, 594, 2175
BbsI	GAAGAC	2 cut(s)	494, 2008
Bbv12I	GWGCWC	2 cut(s)	21, 1857
BbvCI	CCTCAGC	1 cut(s)	63
BccI	CCATC	8 cut(s)	41, 109, 110, 845, 1376, 1667, 1736, 2095
BceAI	ACGGC	2 cut(s)	241, 1305
BcgI	CGANNNNNNTGC	6 cut(s)	1772, 1806, 2084, 2118, 2233, 2267
BciT130I	CCWGG	5 cut(s)	794, 1004, 1121, 1886, 2055
BciVI	GTATCC	1 cut(s)	412
BclI	TGATCA	1 cut(s)	1971
BcnI	CCSGG	1 cut(s)	127
BcoDI	GTCTC	6 cut(s)	686, 896, 946, 983, 1697, 2132
BfaI	CTAG	3 cut(s)	738, 1224, 1412
BfoI	RGCGCY	2 cut(s)	410, 2210
BfrI	CTTAAG	1 cut(s)	554
BfuI	GTATCC	1 cut(s)	412
BglII	AGATCT	1 cut(s)	95
BlpI	GCTNAGC	1 cut(s)	2210
BmcAI	AGTACT	1 cut(s)	1466
Bme1390I	CCNGG	6 cut(s)	127, 794, 1004, 1121, 1886, 2055
Bme18I	GGWCC	2 cut(s)	775, 1315
BmgT120I	GGNCC	9 cut(s)	4, 5, 343, 344, 440, 775, 1315, 1649, 2051
BmiI	GGNNCC	6 cut(s)	6, 345, 643, 784, 1316, 1651
BmrFI	CCNGG	6 cut(s)	127, 794, 1004, 1121, 1886, 2055
BmrI	ACTGGG	2 cut(s)	422, 437
BmsI	GCATC	3 cut(s)	112, 1222, 2112
BmuI	ACTGGG	2 cut(s)	422, 437
BoxI	GACNNNNGTC	1 cut(s)	1200
BpiI	GAAGAC	2 cut(s)	494, 2008
BpmI	CTGGAG	3 cut(s)	585, 677, 2156
Bpu10I	CCTNAGC	2 cut(s)	63, 402
Bpu1102I	GCTNAGC	1 cut(s)	2210
BpuEI	CTTGAG	3 cut(s)	5, 1772, 2159
BpuMI	CCSGG	1 cut(s)	127
Bsa29I	ATCGAT	1 cut(s)	1782
BsaI	GGTCTC	5 cut(s)	686, 896, 983, 1697, 2132
BsaWI	WCCGGW	1 cut(s)	2109
Bse118I	RCCGGY	3 cut(s)	839, 1646, 2203
Bse1I	ACTGG	6 cut(s)	428, 443, 643, 660, 1673, 2256
Bse3DI	GCAATG	1 cut(s)	188
BseBI	CCWGG	5 cut(s)	794, 1004, 1121, 1886, 2055
BseCI	ATCGAT	1 cut(s)	1782
BseGI	GGATG	6 cut(s)	52, 1093, 1659, 1741, 1938, 2067
BseMI	GCAATG	1 cut(s)	188
BseNI	ACTGG	6 cut(s)	428, 443, 643, 660, 1673, 2256
BseRI	GAGGAG	2 cut(s)	1364, 2228
BseSI	GKGCMC	3 cut(s)	8, 347, 1857
BseYI	CCCAGC	2 cut(s)	143, 2247
BshNI	GGYRCC	2 cut(s)	641, 782
BshVI	ATCGAT	1 cut(s)	1782
BsiHKAI	GWGCWC	2 cut(s)	21, 1857
BsiSI	CCGG	5 cut(s)	126, 840, 1647, 2110, 2204
BslFI	GGGAC	3 cut(s)	188, 317, 410
BsmAI	GTCTC	6 cut(s)	686, 896, 946, 983, 1697, 2132
BsmFI	GGGAC	3 cut(s)	188, 317, 410
BsmI	GAATGC	2 cut(s)	632, 722
Bso31I	GGTCTC	5 cut(s)	686, 896, 983, 1697, 2132
Bsp120I	GGGCCC	2 cut(s)	4, 343
Bsp1286I	GDGCHC	6 cut(s)	8, 21, 347, 594, 1857, 2175
Bsp1720I	GCTNAGC	1 cut(s)	2210
Bsp19I	CCATGG	1 cut(s)	258
BspDI	ATCGAT	1 cut(s)	1782
BspLI	GGNNCC	6 cut(s)	6, 345, 643, 784, 1316, 1651
BspPI	GGATC	7 cut(s)	137, 615, 1291, 1534, 1755, 1874, 2120
BspQI	GCTCTTC	1 cut(s)	1401
BspT107I	GGYRCC	2 cut(s)	641, 782
BspTI	CTTAAG	1 cut(s)	554
BspTNI	GGTCTC	5 cut(s)	686, 896, 983, 1697, 2132
BsrDI	GCAATG	1 cut(s)	188
BsrFI	RCCGGY	3 cut(s)	839, 1646, 2203
BsrI	ACTGG	6 cut(s)	428, 443, 643, 660, 1673, 2256
BssAI	RCCGGY	3 cut(s)	839, 1646, 2203
BssT1I	CCWWGG	6 cut(s)	258, 474, 819, 1065, 1071, 1142
Bst2UI	CCWGG	5 cut(s)	794, 1004, 1121, 1886, 2055
Bst4CI	ACNGT	7 cut(s)	424, 1202, 1559, 1588, 1877, 2039, 2168
Bst6I	CTCTTC	4 cut(s)	504, 513, 963, 1401
BstAFI	CTTAAG	1 cut(s)	554
BstC8I	GCNNGC	6 cut(s)	471, 630, 1624, 2127, 2205, 2253
BstDSI	CCRYGG	2 cut(s)	258, 778
BstEII	GGTNACC	1 cut(s)	1147
BstENI	CCTNNNNNAGG	1 cut(s)	1141
BstF5I	GGATG	6 cut(s)	52, 1093, 1659, 1741, 1938, 2067
BstH2I	RGCGCY	2 cut(s)	410, 2210
BstHHI	GCGC	2 cut(s)	409, 2209
BstMAI	GTCTC	6 cut(s)	686, 896, 946, 983, 1697, 2132
BstMWI	GCNNNNNNNGC	8 cut(s)	466, 566, 1031, 1112, 1121, 1178, 1499, 2131
BstNI	CCWGG	5 cut(s)	794, 1004, 1121, 1886, 2055
BstNSI	RCATGY	2 cut(s)	1832, 1915
BstPAI	GACNNNNGTC	1 cut(s)	1200
BstPI	GGTNACC	1 cut(s)	1147
BstSCI	CCNGG	6 cut(s)	125, 792, 1002, 1119, 1884, 2053
BstSLI	GKGCMC	3 cut(s)	8, 347, 1857
BstV2I	GAAGAC	2 cut(s)	494, 2008
BstX2I	RGATCY	4 cut(s)	95, 607, 1526, 1747
BstXI	CCANNNNNNTGG	2 cut(s)	793, 1072
BstYI	RGATCY	4 cut(s)	95, 607, 1526, 1747
Bsu15I	ATCGAT	1 cut(s)	1782
BsuI	GTATCC	1 cut(s)	412
BsuTUI	ATCGAT	1 cut(s)	1782
BtgI	CCRYGG	2 cut(s)	258, 778
BtsCI	GGATG	6 cut(s)	52, 1093, 1659, 1741, 1938, 2067
BtsI	GCAGTG	3 cut(s)	64, 966, 2136
BtsIMutI	CAGTG	8 cut(s)	64, 450, 636, 966, 1774, 1882, 2136, 2263
Cac8I	GCNNGC	6 cut(s)	471, 630, 1624, 2127, 2205, 2253
CaiI	CAGNNNCTG	1 cut(s)	284
CfoI	GCGC	2 cut(s)	409, 2209
Cfr10I	RCCGGY	3 cut(s)	839, 1646, 2203
Cfr13I	GGNCC	9 cut(s)	4, 5, 343, 344, 440, 775, 1315, 1649, 2051
ClaI	ATCGAT	1 cut(s)	1782
CseI	GACGC	1 cut(s)	1970
Csp6I	GTAC	6 cut(s)	783, 829, 1327, 1465, 2035, 2188
CspCI	CAANNNNNGTGG	2 cut(s)	1261, 1296
CviQI	GTAC	6 cut(s)	783, 829, 1327, 1465, 2035, 2188
DrdI	GACNNNNNNGTC	1 cut(s)	1421
DseDI	GACNNNNNNGTC	1 cut(s)	1421
EaeI	YGGCCR	3 cut(s)	261, 1062, 1725
Eam1104I	CTCTTC	4 cut(s)	504, 513, 963, 1401
EarI	CTCTTC	4 cut(s)	504, 513, 963, 1401
EciI	GGCGGA	1 cut(s)	1307
Ecl136II	GAGCTC	1 cut(s)	19
Eco130I	CCWWGG	6 cut(s)	258, 474, 819, 1065, 1071, 1142
Eco147I	AGGCCT	1 cut(s)	1070
Eco24I	GRGCYC	5 cut(s)	8, 21, 347, 594, 2175
Eco31I	GGTCTC	5 cut(s)	686, 896, 983, 1697, 2132
Eco47I	GGWCC	2 cut(s)	775, 1315
Eco53kI	GAGCTC	1 cut(s)	19
Eco57I	CTGAAG	2 cut(s)	1385, 1846
Eco91I	GGTNACC	1 cut(s)	1147
EcoICRI	GAGCTC	1 cut(s)	19
EcoNI	CCTNNNNNAGG	1 cut(s)	1141
EcoO109I	RGGNCCY	2 cut(s)	5, 344
EcoO65I	GGTNACC	1 cut(s)	1147
EcoRII	CCWGG	5 cut(s)	792, 1002, 1119, 1884, 2053
EcoT14I	CCWWGG	6 cut(s)	258, 474, 819, 1065, 1071, 1142
EcoT38I	GRGCYC	5 cut(s)	8, 21, 347, 594, 2175
ErhI	CCWWGG	6 cut(s)	258, 474, 819, 1065, 1071, 1142
FalI	AAGNNNNNCTT	4 cut(s)	818, 850, 918, 950
FaqI	GGGAC	3 cut(s)	188, 317, 410
FauI	CCCGC	2 cut(s)	1631, 2189
FauNDI	CATATG	1 cut(s)	1522
FbaI	TGATCA	1 cut(s)	1971
FokI	GGATG	6 cut(s)	59, 1100, 1646, 1748, 1925, 2054
FriOI	GRGCYC	5 cut(s)	8, 21, 347, 594, 2175
FspBI	CTAG	3 cut(s)	738, 1224, 1412
GlaI	GCGC	2 cut(s)	408, 2208
GsaI	CCCAGC	2 cut(s)	147, 2251
GsuI	CTGGAG	3 cut(s)	585, 677, 2156
HaeII	RGCGCY	2 cut(s)	410, 2210
HapII	CCGG	5 cut(s)	126, 840, 1647, 2110, 2204
HgaI	GACGC	1 cut(s)	1970
HhaI	GCGC	2 cut(s)	409, 2209
Hin6I	GCGC	2 cut(s)	407, 2207
HinP1I	GCGC	2 cut(s)	407, 2207
HincII	GTYRAC	1 cut(s)	976
HindII	GTYRAC	1 cut(s)	976
HindIII	AAGCTT	1 cut(s)	1247
HpaII	CCGG	5 cut(s)	126, 840, 1647, 2110, 2204
HphI	GGTGA	8 cut(s)	242, 745, 1159, 1571, 1648, 1861, 1966, 2167
Hpy166II	GTNNAC	4 cut(s)	355, 976, 1855, 2158
Hpy8I	GTNNAC	4 cut(s)	355, 976, 1855, 2158
Hpy99I	CGWCG	2 cut(s)	1206, 2099
HpyCH4III	ACNGT	7 cut(s)	424, 1202, 1559, 1588, 1877, 2039, 2168
HpyCH4V	TGCA	5 cut(s)	73, 628, 1516, 1855, 2129
HpyF10VI	GCNNNNNNNGC	8 cut(s)	466, 566, 1031, 1112, 1121, 1178, 1499, 2131
HspAI	GCGC	2 cut(s)	407, 2207
KpnI	GGTACC	1 cut(s)	786
KroI	GCCGGC	1 cut(s)	2203
KroNI	GCCGGC	1 cut(s)	2205
Ksp22I	TGATCA	1 cut(s)	1971
LguI	GCTCTTC	1 cut(s)	1401
LmnI	GCTCC	3 cut(s)	566, 2010, 2241
LweI	GCATC	3 cut(s)	112, 1222, 2112
MaeI	CTAG	3 cut(s)	738, 1224, 1412
MaeIII	GTNAC	4 cut(s)	751, 1079, 1147, 1654
MfeI	CAATTG	1 cut(s)	1478
MflI	RGATCY	4 cut(s)	95, 607, 1526, 1747
MhlI	GDGCHC	6 cut(s)	8, 21, 347, 594, 1857, 2175
MlsI	TGGCCA	3 cut(s)	263, 1064, 1727
MluCI	AATT	8 cut(s)	519, 581, 812, 1008, 1041, 1260, 1380, 1478
MluNI	TGGCCA	3 cut(s)	263, 1064, 1727
MlyI	GAGTC	3 cut(s)	668, 1221, 2037
MmeI	TCCRAC	3 cut(s)	642, 901, 1947
Mox20I	TGGCCA	3 cut(s)	263, 1064, 1727
MroNI	GCCGGC	1 cut(s)	2203
MscI	TGGCCA	3 cut(s)	263, 1064, 1727
MseI	TTAA	7 cut(s)	555, 804, 1011, 1053, 1251, 1383, 1395
MslI	CAYNNNNRTG	4 cut(s)	1086, 1203, 1910, 1955
Msp20I	TGGCCA	3 cut(s)	263, 1064, 1727
MspA1I	CMGCKG	2 cut(s)	563, 1115
MspCI	CTTAAG	1 cut(s)	554
MspI	CCGG	5 cut(s)	126, 840, 1647, 2110, 2204
MspR9I	CCNGG	6 cut(s)	127, 794, 1004, 1121, 1886, 2055
MunI	CAATTG	1 cut(s)	1478
Mva1269I	GAATGC	2 cut(s)	632, 722
MvaI	CCWGG	5 cut(s)	794, 1004, 1121, 1886, 2055
MwoI	GCNNNNNNNGC	8 cut(s)	466, 566, 1031, 1112, 1121, 1178, 1499, 2131
NaeI	GCCGGC	1 cut(s)	2205
NciI	CCSGG	1 cut(s)	127
NcoI	CCATGG	1 cut(s)	258
NdeI	CATATG	1 cut(s)	1522
NgoMIV	GCCGGC	1 cut(s)	2203
NlaIV	GGNNCC	6 cut(s)	6, 345, 643, 784, 1316, 1651
NmuCI	GTSAC	4 cut(s)	751, 1079, 1147, 1654
NspI	RCATGY	2 cut(s)	1832, 1915
OliI	CACNNNNGTG	1 cut(s)	1203
PceI	AGGCCT	1 cut(s)	1070
PciI	ACATGT	1 cut(s)	1911
PciSI	GCTCTTC	1 cut(s)	1401
PctI	GAATGC	2 cut(s)	632, 722
PdiI	GCCGGC	1 cut(s)	2205
PfeI	GAWTC	8 cut(s)	35, 240, 577, 614, 1190, 1698, 1900, 2046
PflFI	GACNNNGTC	1 cut(s)	980
PflMI	CCANNNNNTGG	2 cut(s)	215, 1509
PfoI	TCCNGGA	1 cut(s)	1884
PleI	GAGTC	3 cut(s)	667, 1221, 2036
PpsI	GAGTC	3 cut(s)	667, 1221, 2036
PscI	ACATGT	1 cut(s)	1911
PshAI	GACNNNNGTC	1 cut(s)	1200
Psp124BI	GAGCTC	1 cut(s)	21
Psp6I	CCWGG	5 cut(s)	792, 1002, 1119, 1884, 2053
PspEI	GGTNACC	1 cut(s)	1147
PspFI	CCCAGC	2 cut(s)	143, 2247
PspGI	CCWGG	5 cut(s)	792, 1002, 1119, 1884, 2053
PspN4I	GGNNCC	6 cut(s)	6, 345, 643, 784, 1316, 1651
PspOMI	GGGCCC	2 cut(s)	4, 343
PspPI	GGNCC	9 cut(s)	4, 5, 343, 344, 440, 775, 1315, 1649, 2051
PstNI	CAGNNNCTG	1 cut(s)	284
PsuI	RGATCY	4 cut(s)	95, 607, 1526, 1747
PsyI	GACNNNGTC	1 cut(s)	980
PvuII	CAGCTG	1 cut(s)	563
RsaI	GTAC	6 cut(s)	784, 830, 1328, 1466, 2036, 2189
RsaNI	GTAC	6 cut(s)	783, 829, 1327, 1465, 2035, 2188
RseI	CAYNNNNRTG	4 cut(s)	1086, 1203, 1910, 1955
SacI	GAGCTC	1 cut(s)	21
SapI	GCTCTTC	1 cut(s)	1401
SaqAI	TTAA	7 cut(s)	555, 804, 1011, 1053, 1251, 1383, 1395
Sau96I	GGNCC	9 cut(s)	4, 5, 343, 344, 440, 775, 1315, 1649, 2051
ScaI	AGTACT	1 cut(s)	1466
SchI	GAGTC	3 cut(s)	668, 1221, 2037
ScrFI	CCNGG	6 cut(s)	127, 794, 1004, 1121, 1886, 2055
SduI	GDGCHC	6 cut(s)	8, 21, 347, 594, 1857, 2175
SfaNI	GCATC	3 cut(s)	112, 1222, 2112
SinI	GGWCC	2 cut(s)	775, 1315
SmiMI	CAYNNNNRTG	4 cut(s)	1086, 1203, 1910, 1955
SmlI	CTYRAG	4 cut(s)	20, 554, 1751, 2174
SmoI	CTYRAG	4 cut(s)	20, 554, 1751, 2174
Sse9I	AATT	8 cut(s)	519, 581, 812, 1008, 1041, 1260, 1380, 1478
SseBI	AGGCCT	1 cut(s)	1070
SspMI	CTAG	3 cut(s)	738, 1224, 1412
SstI	GAGCTC	1 cut(s)	21
StuI	AGGCCT	1 cut(s)	1070
StyD4I	CCNGG	6 cut(s)	125, 792, 1002, 1119, 1884, 2053
StyI	CCWWGG	6 cut(s)	258, 474, 819, 1065, 1071, 1142
TaaI	ACNGT	7 cut(s)	424, 1202, 1559, 1588, 1877, 2039, 2168
TaqI	TCGA	7 cut(s)	243, 999, 1230, 1531, 1701, 1782, 1953
TasI	AATT	8 cut(s)	519, 581, 812, 1008, 1041, 1260, 1380, 1478
TatI	WGTACW	2 cut(s)	1326, 1464
TauI	GCSGC	4 cut(s)	722, 1115, 1572, 2231
TfiI	GAWTC	8 cut(s)	35, 240, 577, 614, 1190, 1698, 1900, 2046
Tru1I	TTAA	7 cut(s)	555, 804, 1011, 1053, 1251, 1383, 1395
Tru9I	TTAA	7 cut(s)	555, 804, 1011, 1053, 1251, 1383, 1395
TscAI	CASTG	8 cut(s)	64, 450, 643, 973, 1774, 1882, 2136, 2263
TseFI	GTSAC	4 cut(s)	751, 1079, 1147, 1654
Tsp45I	GTSAC	4 cut(s)	751, 1079, 1147, 1654
TspDTI	ATGAA	5 cut(s)	316, 561, 1421, 1646, 1752
TspGWI	ACGGA	2 cut(s)	767, 2009
TspRI	CASTG	8 cut(s)	64, 450, 643, 973, 1774, 1882, 2136, 2263
Tth111I	GACNNNGTC	1 cut(s)	980
Van91I	CCANNNNNTGG	2 cut(s)	215, 1509
Vha464I	CTTAAG	1 cut(s)	554
VneI	GTGCAC	1 cut(s)	1853
VpaK11BI	GGWCC	2 cut(s)	775, 1315
XagI	CCTNNNNNAGG	1 cut(s)	1141
XapI	RAATTY	2 cut(s)	519, 812
XceI	RCATGY	2 cut(s)	1832, 1915
XcmI	CCANNNNNNNNNTGG	2 cut(s)	216, 601
XspI	CTAG	3 cut(s)	738, 1224, 1412
ZrmI	AGTACT	1 cut(s)	1466

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.