FvH4_2g41361

Lariat debranching

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0012955
Physical Location & Seq
Forward (+)
29271916 .. 29273718
1803 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g41361.t1

Sequence Viewer

Length: 987 bp
ATGGAGGAAAGACAAACAACCTTCTCCTTTACACATAAAATCCTCTTGTTAGTGTGGTTATGTCAGGCTTCCTGTGCAAGTGCACACCTCACATTGAACTTCTACTCAACTTCATGCCCTTCTGCTGAACTCATGGTCAGAGGCACAGTCAGATCAGCTTCAAACATTGACCCTACTGTCCCAGGGAAGCTCTTACGGTTGCTCTTCCATGACTGCTTTGTGGAGGGTTGTGATGCCTCTGTGCTTCTACAAGGAAATGGAACTGAGAGAAGTGATCCAGCGAACCAATCTTTGGCAGGATTTTCAGTTATTGATACAGCAAAGAGACTGCTTGAAATTTTATGTCCAAGAACTGTTTCTTGTGCTGACATTGTTGCTTTGGCTGCTAGAGACGCAGTTGAAATTACTGGAGGGCCCCTGGTTCATATTCCAACTGGTAGGCGAGATGGGAAGGTTTCATCAGCTTCAAATGTCAGGCCAAATATTGTAGATACAAGCTTTACAATGAATGAGATGATAAAGCTCTTCTCTTCTAAAGGGTTGTCTTTGCATGACCTTGTAACCCTCTCAGGAGCTCACACTATAGGAGCCGCTCACTGCAGTGCATTCAGTGACCGATTCCAACAAGACTCGAAAGGAAAATTCAGACTCATTGACACATCTTTAGACAATGGTTATGCAGAGAAACTCATGAAACAGTGCCCTGCAGGCGCAAACCCATCTACAACAGTAAAAAATGATCCTGGAACATCCTTTGTGTTTGATAATCAATACTACCGCAATCTGTTGGCCCATAGAGGGCTGTTTCAATCAGATTCTGTACTGTTCACCGACAGTAGATCAAGCCAGCAAGTGGAGAATTTTGCAGAAGACCAAGTTAATTTTTTTGAGAGTTGGGTTCAGTCCTTCTTGAAGCTTACTAGCACTCATGTTAAGACAGGTGATGAAGGGGAAATTCGAACGTTTTGTGCAACAGCTAATACATGA

Protein Analysis

329

Amino Acids

35.82

Weight (kDa)

6.14

Isoelectric Point (pI)

36.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 46 - 291 7.6e-81 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012955)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24800 AT2G24800 AT4G31760
fragaria_vesca FvH4_2g41361
malus_domestica MD08G1051200.v1.1
prunus_persica Prupe.1G397400_v2.0.a1
pyrus_communis pycom15g04080
rosa_chinensis RchiOBHm_Chr6g0299121
rosa_multiflora Rmu_co8172104.1_g000001 Rmu_sc0001434.1_g000019 Rmu_sc0007483.1_g000008
rosa_rugosa Rorug06G0285500
rosa_samantha Rh1CG254300 Rh6AG396500 Rh6BG404900 Rh6CG411000 Rh6DG397600
rosa_wichuraiana Rw6G034710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 176
AccB7I CCANNNNNTGG 2 cut(s) 292, 853
AccBSI CCGCTC 1 cut(s) 593
AciI CCGC 2 cut(s) 591, 778
AclI AACGTT 1 cut(s) 962
AclWI GGATC 2 cut(s) 269, 734
AcsI RAATTY 4 cut(s) 336, 641, 859, 954
AfaI GTAC 1 cut(s) 822
AfiI CCNNNNNNNGG 3 cut(s) 292, 798, 853
AgsI TTSAA 7 cut(s) 97, 162, 335, 401, 468, 809, 913
AjnI CCWGG 3 cut(s) 181, 417, 742
AjuI GAANNNNNNNTTGG 4 cut(s) 275, 307, 340, 372
AleI CACNNNNGTG 1 cut(s) 600
AluBI AGCT 8 cut(s) 158, 190, 464, 498, 523, 575, 916, 977
AluI AGCT 8 cut(s) 158, 190, 464, 498, 523, 575, 916, 977
Alw21I GWGCWC 2 cut(s) 85, 577
Alw26I GTCTC 2 cut(s) 319, 384
Alw44I GTGCAC 1 cut(s) 81
AlwI GGATC 2 cut(s) 269, 734
AlwNI CAGNNNCTG 1 cut(s) 818
AoxI GGCC 3 cut(s) 413, 476, 789
ApaI GGGCCC 1 cut(s) 417
ApaLI GTGCAC 1 cut(s) 81
ApeKI GCWGC 1 cut(s) 383
ApoI RAATTY 4 cut(s) 336, 641, 859, 954
Asp700I GAANNNNTTC 1 cut(s) 355
AspLEI GCGC 1 cut(s) 713
AspS9I GGNCC 3 cut(s) 413, 414, 790
AsuHPI GGTGA 2 cut(s) 820, 953
AsuII TTCGAA 1 cut(s) 958
BaeGI GKGCMC 3 cut(s) 85, 417, 704
BanII GRGCYC 2 cut(s) 417, 577
BbsI GAAGAC 1 cut(s) 876
Bbv12I GWGCWC 2 cut(s) 85, 577
BbvI GCAGC 1 cut(s) 370
BccI CCATC 2 cut(s) 440, 727
BciT130I CCWGG 3 cut(s) 183, 419, 744
BcoDI GTCTC 2 cut(s) 319, 384
BfaI CTAG 2 cut(s) 387, 921
BfmI CTRYAG 3 cut(s) 582, 598, 705
BglI GCCNNNNNGGC 1 cut(s) 708
BisI GCNGC 2 cut(s) 384, 591
BlsI GCNGC 2 cut(s) 385, 592
Bme1390I CCNGG 3 cut(s) 183, 419, 744
BmgT120I GGNCC 3 cut(s) 413, 414, 790
BmiI GGNNCC 3 cut(s) 415, 416, 589
BmrFI CCNGG 3 cut(s) 183, 419, 744
BmsI GCATC 1 cut(s) 223
BpiI GAAGAC 1 cut(s) 876
BpmI CTGGAG 1 cut(s) 429
Bpu14I TTCGAA 1 cut(s) 958
BsaJI CCNNGG 3 cut(s) 181, 182, 417
Bsc4I CCNNNNNNNGG 3 cut(s) 292, 798, 853
Bse1I ACTGG 2 cut(s) 412, 439
BseBI CCWGG 3 cut(s) 183, 419, 744
BseDI CCNNGG 3 cut(s) 181, 182, 417
BseGI GGATG 1 cut(s) 749
BseLI CCNNNNNNNGG 3 cut(s) 292, 798, 853
BseMII CTCAG 2 cut(s) 255, 582
BseNI ACTGG 2 cut(s) 412, 439
BseSI GKGCMC 3 cut(s) 85, 417, 704
BseXI GCAGC 1 cut(s) 370
BshFI GGCC 3 cut(s) 415, 478, 791
BsiHKAI GWGCWC 2 cut(s) 85, 577
BslFI GGGAC 1 cut(s) 164
BslI CCNNNNNNNGG 3 cut(s) 292, 798, 853
BsmAI GTCTC 2 cut(s) 319, 384
BsmBI CGTCTC 1 cut(s) 384
BsmFI GGGAC 1 cut(s) 164
BsmI GAATGC 1 cut(s) 605
BsnI GGCC 3 cut(s) 415, 478, 791
Bsp119I TTCGAA 1 cut(s) 958
Bsp120I GGGCCC 1 cut(s) 413
Bsp1286I GDGCHC 4 cut(s) 85, 417, 577, 704
Bsp143I GATC 4 cut(s) 152, 274, 739, 839
BspACI CCGC 2 cut(s) 591, 778
BspANI GGCC 3 cut(s) 415, 478, 791
BspCNI CTCAG 2 cut(s) 256, 581
BspHI TCATGA 1 cut(s) 690
BspLI GGNNCC 3 cut(s) 415, 416, 589
BspMAI CTGCAG 2 cut(s) 602, 709
BspPI GGATC 2 cut(s) 269, 734
BspQI GCTCTTC 2 cut(s) 209, 530
BspT104I TTCGAA 1 cut(s) 958
BsrBI CCGCTC 1 cut(s) 593
BsrI ACTGG 2 cut(s) 412, 439
BssECI CCNNGG 3 cut(s) 181, 182, 417
BssMI GATC 4 cut(s) 152, 274, 739, 839
Bst2UI CCWGG 3 cut(s) 183, 419, 744
Bst4CI ACNGT 8 cut(s) 148, 178, 198, 355, 699, 730, 825, 836
Bst6I CTCTTC 3 cut(s) 209, 530, 535
BstBI TTCGAA 1 cut(s) 958
BstC8I GCNNGC 2 cut(s) 709, 848
BstDEI CTNAG 2 cut(s) 264, 568
BstF5I GGATG 1 cut(s) 749
BstHHI GCGC 1 cut(s) 713
BstKTI GATC 4 cut(s) 155, 277, 742, 842
BstMAI GTCTC 2 cut(s) 319, 384
BstMBI GATC 4 cut(s) 152, 274, 739, 839
BstMWI GCNNNNNNNGC 4 cut(s) 74, 383, 392, 708
BstNI CCWGG 3 cut(s) 183, 419, 744
BstSCI CCNGG 3 cut(s) 181, 417, 742
BstSFI CTRYAG 3 cut(s) 582, 598, 705
BstSLI GKGCMC 3 cut(s) 85, 417, 704
BstV1I GCAGC 1 cut(s) 370
BstV2I GAAGAC 1 cut(s) 876
BsuRI GGCC 3 cut(s) 415, 478, 791
BtsCI GGATG 1 cut(s) 749
BtsI GCAGTG 2 cut(s) 595, 607
BtsIMutI CAGTG 4 cut(s) 595, 607, 616, 704
Cac8I GCNNGC 2 cut(s) 709, 848
CaiI CAGNNNCTG 1 cut(s) 818
CciI TCATGA 1 cut(s) 690
CfoI GCGC 1 cut(s) 713
Cfr13I GGNCC 3 cut(s) 413, 414, 790
CseI GACGC 1 cut(s) 401
Csp6I GTAC 1 cut(s) 821
CviAII CATG 7 cut(s) 114, 133, 209, 551, 691, 929, 984
CviQI GTAC 1 cut(s) 821
DdeI CTNAG 2 cut(s) 264, 568
DpnI GATC 4 cut(s) 154, 276, 741, 841
DpnII GATC 4 cut(s) 152, 274, 739, 839
DrdI GACNNNNNNGTC 1 cut(s) 176
DseDI GACNNNNNNGTC 1 cut(s) 176
Eam1104I CTCTTC 3 cut(s) 209, 530, 535
EarI CTCTTC 3 cut(s) 209, 530, 535
Ecl136II GAGCTC 1 cut(s) 575
Eco24I GRGCYC 2 cut(s) 417, 577
Eco53kI GAGCTC 1 cut(s) 575
EcoICRI GAGCTC 1 cut(s) 575
EcoO109I RGGNCCY 2 cut(s) 413, 414
EcoRII CCWGG 3 cut(s) 181, 417, 742
EcoT38I GRGCYC 2 cut(s) 417, 577
Esp3I CGTCTC 1 cut(s) 384
FaeI CATG 7 cut(s) 117, 136, 212, 554, 694, 932, 987
FaqI GGGAC 1 cut(s) 164
FatI CATG 7 cut(s) 113, 132, 208, 550, 690, 928, 983
Fnu4HI GCNGC 2 cut(s) 384, 591
FokI GGATG 1 cut(s) 736
FriOI GRGCYC 2 cut(s) 417, 577
Fsp4HI GCNGC 2 cut(s) 384, 591
FspBI CTAG 2 cut(s) 387, 921
GlaI GCGC 1 cut(s) 712
GluI GCNGC 2 cut(s) 384, 591
GsuI CTGGAG 1 cut(s) 429
HaeIII GGCC 3 cut(s) 415, 478, 791
HgaI GACGC 1 cut(s) 401
HhaI GCGC 1 cut(s) 713
Hin1II CATG 7 cut(s) 117, 136, 212, 554, 694, 932, 987
Hin6I GCGC 1 cut(s) 711
HinP1I GCGC 1 cut(s) 711
HindIII AAGCTT 2 cut(s) 496, 914
HinfI GANTC 4 cut(s) 618, 629, 648, 815
HphI GGTGA 2 cut(s) 820, 953
Hpy166II GTNNAC 2 cut(s) 83, 828
Hpy188I TCNGA 4 cut(s) 140, 152, 647, 814
Hpy188III TCNNGA 3 cut(s) 570, 691, 910
Hpy8I GTNNAC 2 cut(s) 83, 828
HpyAV CCTTC 5 cut(s) 31, 129, 445, 916, 941
HpyCH4III ACNGT 8 cut(s) 148, 178, 198, 355, 699, 730, 825, 836
HpyCH4IV ACGT 1 cut(s) 962
HpyCH4V TGCA 9 cut(s) 77, 83, 550, 600, 605, 680, 707, 866, 971
HpyF10VI GCNNNNNNNGC 4 cut(s) 74, 383, 392, 708
HpyF3I CTNAG 2 cut(s) 264, 568
HpySE526I ACGT 1 cut(s) 962
Hsp92II CATG 7 cut(s) 117, 136, 212, 554, 694, 932, 987
HspAI GCGC 1 cut(s) 711
Kzo9I GATC 4 cut(s) 152, 274, 739, 839
LguI GCTCTTC 2 cut(s) 209, 530
LmnI GCTCC 2 cut(s) 572, 587
Lsp1109I GCAGC 1 cut(s) 370
LweI GCATC 1 cut(s) 223
MaeI CTAG 2 cut(s) 387, 921
MaeII ACGT 1 cut(s) 962
MaeIII GTNAC 2 cut(s) 559, 611
MalI GATC 4 cut(s) 154, 276, 741, 841
MbiI CCGCTC 1 cut(s) 593
MboI GATC 4 cut(s) 152, 274, 739, 839
MboII GAAGA 4 cut(s) 196, 517, 522, 881
MhlI GDGCHC 4 cut(s) 85, 417, 577, 704
MluCI AATT 6 cut(s) 336, 402, 641, 859, 880, 954
MlyI GAGTC 2 cut(s) 623, 642
MmeI TCCRAC 2 cut(s) 455, 646
MnlI CCTC 8 cut(s) 53, 98, 134, 217, 247, 404, 575, 791
MroXI GAANNNNTTC 1 cut(s) 355
MseI TTAA 2 cut(s) 879, 933
MslI CAYNNNNRTG 1 cut(s) 600
MspR9I CCNGG 3 cut(s) 183, 419, 744
Mva1269I GAATGC 1 cut(s) 605
MvaI CCWGG 3 cut(s) 183, 419, 744
MwoI GCNNNNNNNGC 4 cut(s) 74, 383, 392, 708
NdeII GATC 4 cut(s) 152, 274, 739, 839
NlaIII CATG 7 cut(s) 117, 136, 212, 554, 694, 932, 987
NlaIV GGNNCC 3 cut(s) 415, 416, 589
NmuCI GTSAC 1 cut(s) 611
NspV TTCGAA 1 cut(s) 958
OliI CACNNNNGTG 1 cut(s) 600
PagI TCATGA 1 cut(s) 690
PasI CCCWGGG 1 cut(s) 182
PciSI GCTCTTC 2 cut(s) 209, 530
PctI GAATGC 1 cut(s) 605
PdmI GAANNNNTTC 1 cut(s) 355
PfeI GAWTC 2 cut(s) 618, 815
PflMI CCANNNNNTGG 2 cut(s) 292, 853
PfoI TCCNGGA 1 cut(s) 742
PkrI GCNGC 2 cut(s) 385, 592
PleI GAGTC 2 cut(s) 623, 642
PpsI GAGTC 2 cut(s) 623, 642
Psp124BI GAGCTC 1 cut(s) 577
Psp1406I AACGTT 1 cut(s) 962
Psp6I CCWGG 3 cut(s) 181, 417, 742
PspGI CCWGG 3 cut(s) 181, 417, 742
PspN4I GGNNCC 3 cut(s) 415, 416, 589
PspOMI GGGCCC 1 cut(s) 413
PspPI GGNCC 3 cut(s) 413, 414, 790
PstI CTGCAG 2 cut(s) 602, 709
PstNI CAGNNNCTG 1 cut(s) 818
RsaI GTAC 1 cut(s) 822
RsaNI GTAC 1 cut(s) 821
RseI CAYNNNNRTG 1 cut(s) 600
SacI GAGCTC 1 cut(s) 577
SapI GCTCTTC 2 cut(s) 209, 530
SaqAI TTAA 2 cut(s) 879, 933
SatI GCNGC 2 cut(s) 384, 591
Sau3AI GATC 4 cut(s) 152, 274, 739, 839
Sau96I GGNCC 3 cut(s) 413, 414, 790
SbfI CCTGCAGG 1 cut(s) 709
SchI GAGTC 2 cut(s) 623, 642
ScrFI CCNGG 3 cut(s) 183, 419, 744
SdaI CCTGCAGG 1 cut(s) 709
SduI GDGCHC 4 cut(s) 85, 417, 577, 704
SfaNI GCATC 1 cut(s) 223
SfcI CTRYAG 3 cut(s) 582, 598, 705
SfuI TTCGAA 1 cut(s) 958
SmiMI CAYNNNNRTG 1 cut(s) 600
Sse8387I CCTGCAGG 1 cut(s) 709
Sse9I AATT 6 cut(s) 336, 402, 641, 859, 880, 954
SsiI CCGC 2 cut(s) 591, 778
SspI AATATT 1 cut(s) 484
SspMI CTAG 2 cut(s) 387, 921
SstI GAGCTC 1 cut(s) 577
StyD4I CCNGG 3 cut(s) 181, 417, 742
TaaI ACNGT 8 cut(s) 148, 178, 198, 355, 699, 730, 825, 836
TaiI ACGT 1 cut(s) 965
TaqI TCGA 2 cut(s) 632, 958
TaqII GACCGA 1 cut(s) 630
TasI AATT 6 cut(s) 336, 402, 641, 859, 880, 954
TatI WGTACW 1 cut(s) 820
TauI GCSGC 1 cut(s) 593
TfiI GAWTC 2 cut(s) 618, 815
Tru1I TTAA 2 cut(s) 879, 933
Tru9I TTAA 2 cut(s) 879, 933
TscAI CASTG 4 cut(s) 602, 607, 616, 704
TseFI GTSAC 1 cut(s) 611
TseI GCWGC 1 cut(s) 383
Tsp45I GTSAC 1 cut(s) 611
TspDTI ATGAA 6 cut(s) 102, 413, 447, 521, 707, 960
TspRI CASTG 4 cut(s) 602, 607, 616, 704
Van91I CCANNNNNTGG 2 cut(s) 292, 853
VneI GTGCAC 1 cut(s) 81
XapI RAATTY 4 cut(s) 336, 641, 859, 954
XmnI GAANNNNTTC 1 cut(s) 355
XspI CTAG 2 cut(s) 387, 921
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.