Rorug06G0285500

Lariat debranching

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0012955
Physical Location & Seq
Forward (+)
43571798 .. 43572616
819 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0285500.1

Sequence Viewer

Length: 507 bp
ATGGAATTGATAATTCATATCTTATCAAATCAGCTAGCTAGCTCCCCAGAAACAAGAATGAGAGCTCTCAAAGGCATTCTCGTTTTCCATTTGTGTGCAGCTTTGGCCCTGAGTATTTGCTCGAGATCCTCTGCCAAATGGCCCGGTCTTGATCCCACCAACTGGCATGTGCACGTCGTTAACGGTCTCAGTGGACAAAGAGCATTGGTTGTCCATTGTTGGTCCGACCTCGACGATCTTGGCACCCATACTCTTTATCCTGGTGGCGACTTCAACTGGCATTTCCATGTCAACTTGCCCGGGACAACGCGTTTCACTTGCGACTTCCATGCAGACAAACAACACGCTTTGTTTGATGTGTTTTGGAAGGAGAGCAAACATATGTGGCTTCGGACCCATTGCAACTGGAAAGCATGTTTTTGGATTGCGAAAGATGATGGGATTTACCTCAAAAACGTTCCGGAGAATCGTGACGACCGTATTCATCCATGGGAAACACGATTGTGA

Protein Analysis

168

Amino Acids

19.45

Weight (kDa)

7.21

Isoelectric Point (pI)

32.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Self-incomp_S1 PF05938 56 - 164 2.6e-27 Plant self-incompatibility protein S1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012955)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24800 AT2G24800 AT4G31760
fragaria_vesca FvH4_2g41361
malus_domestica MD08G1051200.v1.1
prunus_persica Prupe.1G397400_v2.0.a1
pyrus_communis pycom15g04080
rosa_chinensis RchiOBHm_Chr6g0299121
rosa_multiflora Rmu_co8172104.1_g000001 Rmu_sc0001434.1_g000019 Rmu_sc0007483.1_g000008
rosa_rugosa Rorug06G0285500
rosa_samantha Rh1CG254300 Rh6AG396500 Rh6BG404900 Rh6CG411000 Rh6DG397600
rosa_wichuraiana Rw6G034710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 242
AccB7I CCANNNNNTGG 1 cut(s) 162
AccII CGCG 1 cut(s) 310
AccIII TCCGGA 1 cut(s) 460
AclI AACGTT 1 cut(s) 456
AclWI GGATC 2 cut(s) 120, 146
AfiI CCNNNNNNNGG 1 cut(s) 162
AflIII ACRYGT 1 cut(s) 308
AgsI TTSAA 1 cut(s) 274
AjiI CACGTC 1 cut(s) 175
AjnI CCWGG 1 cut(s) 259
AleI CACNNNNGTG 1 cut(s) 502
AluBI AGCT 5 cut(s) 34, 38, 42, 65, 101
AluI AGCT 5 cut(s) 34, 38, 42, 65, 101
Alw21I GWGCWC 2 cut(s) 67, 174
Alw26I GTCTC 1 cut(s) 191
Alw44I GTGCAC 1 cut(s) 170
AlwI GGATC 2 cut(s) 120, 146
Ama87I CYCGRG 2 cut(s) 121, 299
Aor13HI TCCGGA 1 cut(s) 460
AoxI GGCC 2 cut(s) 105, 140
ApaLI GTGCAC 1 cut(s) 170
ApeKI GCWGC 1 cut(s) 98
AspS9I GGNCC 4 cut(s) 106, 141, 222, 393
AsuC2I CCSGG 3 cut(s) 144, 300, 301
AsuNHI GCTAGC 2 cut(s) 34, 38
AvaI CYCGRG 2 cut(s) 121, 299
AvaII GGWCC 2 cut(s) 222, 393
BaeGI GKGCMC 1 cut(s) 174
BanI GGYRCC 1 cut(s) 242
BanII GRGCYC 1 cut(s) 67
Bbv12I GWGCWC 2 cut(s) 67, 174
BbvI GCAGC 1 cut(s) 110
BccI CCATC 1 cut(s) 431
BcgI CGANNNNNNTGC 2 cut(s) 311, 345
BciT130I CCWGG 1 cut(s) 261
BcnI CCSGG 3 cut(s) 144, 300, 301
BcoDI GTCTC 1 cut(s) 191
BfaI CTAG 2 cut(s) 35, 39
BisI GCNGC 1 cut(s) 99
BlsI GCNGC 1 cut(s) 100
Bme1390I CCNGG 4 cut(s) 144, 261, 300, 301
Bme18I GGWCC 2 cut(s) 222, 393
BmeT110I CYCGRG 2 cut(s) 121, 299
BmgBI CACGTC 1 cut(s) 175
BmgT120I GGNCC 4 cut(s) 106, 141, 222, 393
BmiI GGNNCC 2 cut(s) 244, 395
BmrFI CCNGG 4 cut(s) 144, 261, 300, 301
BmtI GCTAGC 2 cut(s) 38, 42
BpuMI CCSGG 3 cut(s) 144, 300, 301
BsaI GGTCTC 1 cut(s) 191
BsaJI CCNNGG 2 cut(s) 299, 488
BsaWI WCCGGW 1 cut(s) 460
Bsc4I CCNNNNNNNGG 1 cut(s) 162
Bse1I ACTGG 3 cut(s) 167, 281, 410
Bse3DI GCAATG 1 cut(s) 397
BseAI TCCGGA 1 cut(s) 460
BseBI CCWGG 1 cut(s) 261
BseDI CCNNGG 2 cut(s) 299, 488
BseGI GGATG 1 cut(s) 484
BseLI CCNNNNNNNGG 1 cut(s) 162
BseMI GCAATG 1 cut(s) 397
BseMII CTCAG 2 cut(s) 101, 202
BseNI ACTGG 3 cut(s) 167, 281, 410
BseSI GKGCMC 1 cut(s) 174
BseXI GCAGC 1 cut(s) 110
BsgI GTGCAG 1 cut(s) 117
Bsh1236I CGCG 1 cut(s) 310
Bsh1285I CGRYCG 1 cut(s) 478
BshFI GGCC 2 cut(s) 107, 142
BshNI GGYRCC 1 cut(s) 242
BsiEI CGRYCG 1 cut(s) 478
BsiHKAI GWGCWC 2 cut(s) 67, 174
BsiHKCI CYCGRG 2 cut(s) 121, 299
BsiSI CCGG 3 cut(s) 144, 300, 461
BslFI GGGAC 1 cut(s) 316
BslI CCNNNNNNNGG 1 cut(s) 162
BsmAI GTCTC 1 cut(s) 191
BsmFI GGGAC 1 cut(s) 316
BsmI GAATGC 1 cut(s) 75
BsnI GGCC 2 cut(s) 107, 142
Bso31I GGTCTC 1 cut(s) 191
BsoBI CYCGRG 2 cut(s) 121, 299
Bsp1286I GDGCHC 2 cut(s) 67, 174
Bsp13I TCCGGA 1 cut(s) 460
Bsp143I GATC 3 cut(s) 125, 151, 235
Bsp19I CCATGG 1 cut(s) 488
BspANI GGCC 2 cut(s) 107, 142
BspCNI CTCAG 2 cut(s) 102, 201
BspEI TCCGGA 1 cut(s) 460
BspFNI CGCG 1 cut(s) 310
BspLI GGNNCC 2 cut(s) 244, 395
BspOI GCTAGC 2 cut(s) 38, 42
BspPI GGATC 2 cut(s) 120, 146
BspT107I GGYRCC 1 cut(s) 242
BspTNI GGTCTC 1 cut(s) 191
BsrDI GCAATG 1 cut(s) 397
BsrI ACTGG 3 cut(s) 167, 281, 410
BssECI CCNNGG 2 cut(s) 299, 488
BssMI GATC 3 cut(s) 125, 151, 235
BssT1I CCWWGG 1 cut(s) 488
Bst2UI CCWGG 1 cut(s) 261
Bst4CI ACNGT 2 cut(s) 185, 479
BstC8I GCNNGC 2 cut(s) 36, 40
BstDEI CTNAG 2 cut(s) 110, 188
BstDSI CCRYGG 1 cut(s) 488
BstF5I GGATG 1 cut(s) 484
BstFNI CGCG 1 cut(s) 310
BstKTI GATC 3 cut(s) 128, 154, 238
BstMAI GTCTC 1 cut(s) 191
BstMBI GATC 3 cut(s) 125, 151, 235
BstMCI CGRYCG 1 cut(s) 478
BstMWI GCNNNNNNNGC 1 cut(s) 104
BstNI CCWGG 1 cut(s) 261
BstNSI RCATGY 2 cut(s) 170, 417
BstSCI CCNGG 4 cut(s) 142, 259, 298, 299
BstSLI GKGCMC 1 cut(s) 174
BstUI CGCG 1 cut(s) 310
BstV1I GCAGC 1 cut(s) 110
BstX2I RGATCY 1 cut(s) 125
BstYI RGATCY 1 cut(s) 125
BsuRI GGCC 2 cut(s) 107, 142
BtgI CCRYGG 1 cut(s) 488
BtrI CACGTC 1 cut(s) 175
BtsCI GGATG 1 cut(s) 484
BtsIMutI CAGTG 1 cut(s) 196
Cac8I GCNNGC 2 cut(s) 36, 40
Cfr13I GGNCC 4 cut(s) 106, 141, 222, 393
Cfr9I CCCGGG 1 cut(s) 299
CviAII CATG 5 cut(s) 167, 287, 329, 414, 489
CviJI RGCY 8 cut(s) 34, 38, 42, 65, 101, 107, 142, 388
CviKI_1 RGCY 8 cut(s) 34, 38, 42, 65, 101, 107, 142, 388
DdeI CTNAG 2 cut(s) 110, 188
DpnI GATC 3 cut(s) 127, 153, 237
DpnII GATC 3 cut(s) 125, 151, 235
Ecl136II GAGCTC 1 cut(s) 65
Eco130I CCWWGG 1 cut(s) 488
Eco24I GRGCYC 1 cut(s) 67
Eco31I GGTCTC 1 cut(s) 191
Eco47I GGWCC 2 cut(s) 222, 393
Eco53kI GAGCTC 1 cut(s) 65
Eco88I CYCGRG 2 cut(s) 121, 299
EcoICRI GAGCTC 1 cut(s) 65
EcoRII CCWGG 1 cut(s) 259
EcoT14I CCWWGG 1 cut(s) 488
EcoT38I GRGCYC 1 cut(s) 67
ErhI CCWWGG 1 cut(s) 488
FaeI CATG 5 cut(s) 170, 290, 332, 417, 492
FaiI YATR 9 cut(s) 18, 168, 249, 288, 330, 381, 383, 415, 490
FaqI GGGAC 1 cut(s) 316
FatI CATG 5 cut(s) 166, 286, 328, 413, 488
FauNDI CATATG 1 cut(s) 381
Fnu4HI GCNGC 1 cut(s) 99
FokI GGATG 1 cut(s) 471
FriOI GRGCYC 1 cut(s) 67
Fsp4HI GCNGC 1 cut(s) 99
FspBI CTAG 2 cut(s) 35, 39
GluI GCNGC 1 cut(s) 99
HaeIII GGCC 2 cut(s) 107, 142
HapII CCGG 3 cut(s) 144, 300, 461
Hin1II CATG 5 cut(s) 170, 290, 332, 417, 492
HincII GTYRAC 2 cut(s) 181, 292
HindII GTYRAC 2 cut(s) 181, 292
HinfI GANTC 1 cut(s) 466
HpaI GTTAAC 1 cut(s) 181
HpaII CCGG 3 cut(s) 144, 300, 461
Hpy166II GTNNAC 4 cut(s) 172, 181, 194, 292
Hpy188I TCNGA 2 cut(s) 226, 393
Hpy188III TCNNGA 4 cut(s) 123, 149, 461, 470
Hpy8I GTNNAC 4 cut(s) 172, 181, 194, 292
Hpy99I CGWCG 2 cut(s) 179, 236
HpyAV CCTTC 1 cut(s) 361
HpyCH4III ACNGT 2 cut(s) 185, 479
HpyCH4IV ACGT 2 cut(s) 174, 456
HpyCH4V TGCA 4 cut(s) 98, 172, 332, 402
HpyF10VI GCNNNNNNNGC 1 cut(s) 104
HpyF3I CTNAG 2 cut(s) 110, 188
HpySE526I ACGT 2 cut(s) 174, 456
Hsp92II CATG 5 cut(s) 170, 290, 332, 417, 492
Kpn2I TCCGGA 1 cut(s) 460
KspAI GTTAAC 1 cut(s) 181
Kzo9I GATC 3 cut(s) 125, 151, 235
LmnI GCTCC 1 cut(s) 47
Lsp1109I GCAGC 1 cut(s) 110
MaeI CTAG 2 cut(s) 35, 39
MaeII ACGT 2 cut(s) 174, 456
MaeIII GTNAC 1 cut(s) 470
MalI GATC 3 cut(s) 127, 153, 237
MboI GATC 3 cut(s) 125, 151, 235
MflI RGATCY 1 cut(s) 125
MhlI GDGCHC 2 cut(s) 67, 174
MluCI AATT 2 cut(s) 5, 12
MluI ACGCGT 1 cut(s) 308
MmeI TCCRAC 1 cut(s) 249
MnlI CCTC 3 cut(s) 139, 239, 458
MroI TCCGGA 1 cut(s) 460
MseI TTAA 1 cut(s) 180
MslI CAYNNNNRTG 3 cut(s) 93, 285, 502
MspI CCGG 3 cut(s) 144, 300, 461
MspR9I CCNGG 4 cut(s) 144, 261, 300, 301
Mva1269I GAATGC 1 cut(s) 75
MvaI CCWGG 1 cut(s) 261
MvnI CGCG 1 cut(s) 310
MwoI GCNNNNNNNGC 1 cut(s) 104
NciI CCSGG 3 cut(s) 144, 300, 301
NcoI CCATGG 1 cut(s) 488
NdeI CATATG 1 cut(s) 381
NdeII GATC 3 cut(s) 125, 151, 235
NheI GCTAGC 2 cut(s) 34, 38
NlaIII CATG 5 cut(s) 170, 290, 332, 417, 492
NlaIV GGNNCC 2 cut(s) 244, 395
NmuCI GTSAC 1 cut(s) 470
NspI RCATGY 2 cut(s) 170, 417
OliI CACNNNNGTG 1 cut(s) 502
PaeR7I CTCGAG 1 cut(s) 121
PcsI WCGNNNNNNNCGW 1 cut(s) 475
PctI GAATGC 1 cut(s) 75
PfeI GAWTC 1 cut(s) 466
PflMI CCANNNNNTGG 1 cut(s) 162
PkrI GCNGC 1 cut(s) 100
Psp124BI GAGCTC 1 cut(s) 67
Psp1406I AACGTT 1 cut(s) 456
Psp6I CCWGG 1 cut(s) 259
PspGI CCWGG 1 cut(s) 259
PspN4I GGNNCC 2 cut(s) 244, 395
PspPI GGNCC 4 cut(s) 106, 141, 222, 393
PsuI RGATCY 1 cut(s) 125
RseI CAYNNNNRTG 3 cut(s) 93, 285, 502
SacI GAGCTC 1 cut(s) 67
SaqAI TTAA 1 cut(s) 180
SatI GCNGC 1 cut(s) 99
Sau3AI GATC 3 cut(s) 125, 151, 235
Sau96I GGNCC 4 cut(s) 106, 141, 222, 393
ScrFI CCNGG 4 cut(s) 144, 261, 300, 301
SduI GDGCHC 2 cut(s) 67, 174
SetI ASST 9 cut(s) 36, 40, 44, 67, 103, 177, 231, 450, 459
Sfr274I CTCGAG 1 cut(s) 121
SinI GGWCC 2 cut(s) 222, 393
SlaI CTCGAG 1 cut(s) 121
SmaI CCCGGG 1 cut(s) 301
SmiMI CAYNNNNRTG 3 cut(s) 93, 285, 502
SmlI CTYRAG 1 cut(s) 121
SmoI CTYRAG 1 cut(s) 121
Sse9I AATT 2 cut(s) 5, 12
SspMI CTAG 2 cut(s) 35, 39
SstI GAGCTC 1 cut(s) 67
StyD4I CCNGG 4 cut(s) 142, 259, 298, 299
StyI CCWWGG 1 cut(s) 488
TaaI ACNGT 2 cut(s) 185, 479
TaiI ACGT 2 cut(s) 177, 459
TaqI TCGA 2 cut(s) 122, 231
TasI AATT 2 cut(s) 5, 12
TfiI GAWTC 1 cut(s) 466
Tru1I TTAA 1 cut(s) 180
Tru9I TTAA 1 cut(s) 180
TscAI CASTG 1 cut(s) 196
TseFI GTSAC 1 cut(s) 470
TseI GCWGC 1 cut(s) 98
Tsp45I GTSAC 1 cut(s) 470
TspDTI ATGAA 2 cut(s) 5, 473
TspMI CCCGGG 1 cut(s) 299
TspRI CASTG 1 cut(s) 196
Van91I CCANNNNNTGG 1 cut(s) 162
VneI GTGCAC 1 cut(s) 170
VpaK11BI GGWCC 2 cut(s) 222, 393
XceI RCATGY 2 cut(s) 170, 417
XhoI CTCGAG 1 cut(s) 121
XmaI CCCGGG 1 cut(s) 299
XspI CTAG 2 cut(s) 35, 39
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.