MD08G1051200.v1.1

Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0012955
Physical Location & Seq
Reverse (-)
4054705 .. 4057058
2354 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1051200.v1.1.491

Sequence Viewer

Length: 990 bp
ATGGAGAAGACATCGGCAGCCTTCTCTTGCACACATTGTTGTGTTTTCTTAATTTGGTTATTTCTTGCAGCTTCTGCTTCCACTGCAGACCTGGCACTGAATTTCTACGCAGCTTCATGCCCTTCTGCCGAGTTTATTGTCCGAAACACAGTGAGATCGGCCTCCGACTTGGACCCTACCGTCCCCGGGAAGCTCCTCCGTCTGCTCTTCCATGATTGCTTTGTGGAGGGTTGTGATGCATCTGTGCTTCTACAAGGAACTGGGACTGAGAGAAGTGATCCGGCAAACACATCTCTGGGAGGGTTTGCAGTGATCGATTCGGCGAAGAGACTGCTTGAAGTCTTCTGTCCCGGAACTGTTTCTTGCGCTGACATTGTTGCTCTGGCTGCTAGAGATGCTGTTGAATTTTCTGGGGGACCTGTGGCTCAGATTCCAACAGGTAGGAGAGACGGGAAAGTTTCTGCAGCTTCAAATGTCAGGCCTAATATTGTAGATACAAGTTTTACAATGGATGAGATGATTAAGCTCTTCTCTTCCAAAGGGTTGTCTTTGGTTGACCTAGCCACCCTATCAGGAGCTCACACTATCGGATCAGCTCACTGCAGTGCATTCAGAGATCGGTTCAAGCAGGACCCCAAAGGAAAATTCACACTCATTGACACATCCTTAGACAATGTTTATGCAGAGGAGCTCATGGAGCAGTGCCCTGCAGGCGCAAATCAGTCTACAACGGTAAAAAATGATCCTAAAACATCGTTTGCGTTCGATAATCAGTACTACCAAAATCTCTTAGCACACAGAGGGCTGTTTCAATCGGATTCCATCTTGCTAACCGATGGCAGAACAAGAAAACAAGTGGAGATATTTGCACAGGACCAAGTTAGTTTTTTCCAGAGCTGGACTGAGTCATTCTTGAAACTTGCTAGCATCGGCGTGAAGACAGGTGATGAAGGCGAAATTCGAGCCTCTTGTCCGACGACTAATGCCTGA

Protein Analysis

330

Amino Acids

35.39

Weight (kDa)

5.19

Isoelectric Point (pI)

33.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 47 - 292 9.5e-80 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012955)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24800 AT2G24800 AT4G31760
fragaria_vesca FvH4_2g41361
malus_domestica MD08G1051200.v1.1
prunus_persica Prupe.1G397400_v2.0.a1
pyrus_communis pycom15g04080
rosa_chinensis RchiOBHm_Chr6g0299121
rosa_multiflora Rmu_co8172104.1_g000001 Rmu_sc0001434.1_g000019 Rmu_sc0007483.1_g000008
rosa_rugosa Rorug06G0285500
rosa_samantha Rh1CG254300 Rh6AG396500 Rh6BG404900 Rh6CG411000 Rh6DG397600
rosa_wichuraiana Rw6G034710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 179
AccI GTMKAC 1 cut(s) 725
AclWI GGATC 3 cut(s) 272, 598, 737
AcsI RAATTY 4 cut(s) 100, 404, 644, 957
AfaI GTAC 1 cut(s) 776
AfiI CCNNNNNNNGG 1 cut(s) 186
AgsI TTSAA 6 cut(s) 338, 404, 471, 625, 812, 916
AjnI CCWGG 1 cut(s) 90
AjuI GAANNNNNNNTTGG 2 cut(s) 629, 661
AleI CACNNNNGTG 1 cut(s) 603
AluBI AGCT 9 cut(s) 71, 113, 193, 467, 526, 578, 596, 691, 897
AluI AGCT 9 cut(s) 71, 113, 193, 467, 526, 578, 596, 691, 897
Alw21I GWGCWC 2 cut(s) 580, 693
Alw26I GTCTC 2 cut(s) 322, 441
AlwI GGATC 3 cut(s) 272, 598, 737
AlwNI CAGNNNCTG 1 cut(s) 74
Ama87I CYCGRG 1 cut(s) 185
AoxI GGCC 2 cut(s) 159, 479
ApeKI GCWGC 5 cut(s) 17, 68, 110, 386, 464
ApoI RAATTY 4 cut(s) 100, 404, 644, 957
Asp700I GAANNNNTTC 1 cut(s) 358
AspLEI GCGC 2 cut(s) 368, 716
AspS9I GGNCC 4 cut(s) 172, 416, 631, 874
AsuC2I CCSGG 3 cut(s) 186, 187, 351
AsuHPI GGTGA 1 cut(s) 956
AsuNHI GCTAGC 1 cut(s) 923
AvaI CYCGRG 1 cut(s) 185
AvaII GGWCC 4 cut(s) 172, 416, 631, 874
BaeGI GKGCMC 1 cut(s) 707
BanII GRGCYC 2 cut(s) 580, 693
BbsI GAAGAC 3 cut(s) 14, 334, 944
Bbv12I GWGCWC 2 cut(s) 580, 693
BbvI GCAGC 5 cut(s) 29, 80, 122, 373, 476
BccI CCATC 2 cut(s) 830, 830
BcgI CGANNNNNNTGC 2 cut(s) 313, 347
BciT130I CCWGG 1 cut(s) 92
BcnI CCSGG 3 cut(s) 186, 187, 351
BcoDI GTCTC 2 cut(s) 322, 441
BfaI CTAG 3 cut(s) 390, 560, 924
BfmI CTRYAG 4 cut(s) 84, 462, 601, 708
BglI GCCNNNNNGGC 1 cut(s) 711
BisI GCNGC 5 cut(s) 18, 69, 111, 387, 465
BlsI GCNGC 5 cut(s) 19, 70, 112, 388, 466
BmcAI AGTACT 1 cut(s) 776
Bme1390I CCNGG 4 cut(s) 92, 186, 187, 351
Bme18I GGWCC 4 cut(s) 172, 416, 631, 874
BmeT110I CYCGRG 1 cut(s) 185
BmgT120I GGNCC 4 cut(s) 172, 416, 631, 874
BmiI GGNNCC 3 cut(s) 174, 417, 633
BmrFI CCNGG 4 cut(s) 92, 186, 187, 351
BmrI ACTGGG 1 cut(s) 270
BmsI GCATC 4 cut(s) 226, 248, 385, 936
BmtI GCTAGC 1 cut(s) 927
BmuI ACTGGG 1 cut(s) 270
BpiI GAAGAC 3 cut(s) 14, 334, 944
BpuMI CCSGG 3 cut(s) 186, 187, 351
Bsa29I ATCGAT 1 cut(s) 315
BsaJI CCNNGG 2 cut(s) 184, 185
Bsc4I CCNNNNNNNGG 1 cut(s) 186
Bse1I ACTGG 1 cut(s) 265
BseBI CCWGG 1 cut(s) 92
BseCI ATCGAT 1 cut(s) 315
BseDI CCNNGG 2 cut(s) 184, 185
BseGI GGATG 2 cut(s) 517, 662
BseLI CCNNNNNNNGG 1 cut(s) 186
BseMII CTCAG 3 cut(s) 258, 440, 894
BseNI ACTGG 1 cut(s) 265
BseRI GAGGAG 2 cut(s) 185, 701
BseSI GKGCMC 1 cut(s) 707
BseXI GCAGC 5 cut(s) 29, 80, 122, 373, 476
BshFI GGCC 2 cut(s) 161, 481
BshVI ATCGAT 1 cut(s) 315
BsiHKAI GWGCWC 2 cut(s) 580, 693
BsiHKCI CYCGRG 1 cut(s) 185
BsiSI CCGG 3 cut(s) 186, 281, 351
BslFI GGGAC 4 cut(s) 167, 277, 333, 429
BslI CCNNNNNNNGG 1 cut(s) 186
BsmAI GTCTC 2 cut(s) 322, 441
BsmBI CGTCTC 1 cut(s) 441
BsmFI GGGAC 4 cut(s) 167, 277, 333, 429
BsmI GAATGC 1 cut(s) 608
BsnI GGCC 2 cut(s) 161, 481
BsoBI CYCGRG 1 cut(s) 185
Bsp1286I GDGCHC 3 cut(s) 580, 693, 707
Bsp143I GATC 6 cut(s) 155, 277, 312, 590, 616, 742
BspANI GGCC 2 cut(s) 161, 481
BspCNI CTCAG 3 cut(s) 259, 439, 895
BspDI ATCGAT 1 cut(s) 315
BspLI GGNNCC 3 cut(s) 174, 417, 633
BspMAI CTGCAG 4 cut(s) 88, 466, 605, 712
BspOI GCTAGC 1 cut(s) 927
BspPI GGATC 3 cut(s) 272, 598, 737
BspQI GCTCTTC 2 cut(s) 212, 533
BsrI ACTGG 1 cut(s) 265
BssECI CCNNGG 2 cut(s) 184, 185
BssMI GATC 6 cut(s) 155, 277, 312, 590, 616, 742
Bst2UI CCWGG 1 cut(s) 92
Bst4CI ACNGT 4 cut(s) 151, 181, 358, 733
Bst6I CTCTTC 4 cut(s) 212, 320, 533, 538
BstAPI GCANNNNNTGC 1 cut(s) 74
BstC8I GCNNGC 2 cut(s) 712, 925
BstDEI CTNAG 5 cut(s) 267, 426, 667, 790, 903
BstF5I GGATG 2 cut(s) 517, 662
BstHHI GCGC 2 cut(s) 368, 716
BstKTI GATC 6 cut(s) 158, 280, 315, 593, 619, 745
BstMAI GTCTC 2 cut(s) 322, 441
BstMBI GATC 6 cut(s) 155, 277, 312, 590, 616, 742
BstMWI GCNNNNNNNGC 7 cut(s) 74, 83, 92, 386, 395, 697, 711
BstNI CCWGG 1 cut(s) 92
BstSCI CCNGG 4 cut(s) 90, 184, 185, 349
BstSFI CTRYAG 4 cut(s) 84, 462, 601, 708
BstSLI GKGCMC 1 cut(s) 707
BstV1I GCAGC 5 cut(s) 29, 80, 122, 373, 476
BstV2I GAAGAC 3 cut(s) 14, 334, 944
Bsu15I ATCGAT 1 cut(s) 315
BsuRI GGCC 2 cut(s) 161, 481
BsuTUI ATCGAT 1 cut(s) 315
BtsCI GGATG 2 cut(s) 517, 662
BtsI GCAGTG 5 cut(s) 81, 315, 598, 610, 707
BtsIMutI CAGTG 7 cut(s) 81, 95, 156, 315, 598, 610, 707
Cac8I GCNNGC 2 cut(s) 712, 925
CaiI CAGNNNCTG 1 cut(s) 74
CfoI GCGC 2 cut(s) 368, 716
Cfr13I GGNCC 4 cut(s) 172, 416, 631, 874
Cfr9I CCCGGG 1 cut(s) 185
ClaI ATCGAT 1 cut(s) 315
Csp6I GTAC 1 cut(s) 775
CviAII CATG 3 cut(s) 117, 212, 694
CviQI GTAC 1 cut(s) 775
DdeI CTNAG 5 cut(s) 267, 426, 667, 790, 903
DpnI GATC 6 cut(s) 157, 279, 314, 592, 618, 744
DpnII GATC 6 cut(s) 155, 277, 312, 590, 616, 742
DrdI GACNNNNNNGTC 1 cut(s) 179
DseDI GACNNNNNNGTC 1 cut(s) 179
Eam1104I CTCTTC 4 cut(s) 212, 320, 533, 538
EarI CTCTTC 4 cut(s) 212, 320, 533, 538
Ecl136II GAGCTC 2 cut(s) 578, 691
Eco147I AGGCCT 1 cut(s) 481
Eco24I GRGCYC 2 cut(s) 580, 693
Eco47I GGWCC 4 cut(s) 172, 416, 631, 874
Eco53kI GAGCTC 2 cut(s) 578, 691
Eco88I CYCGRG 1 cut(s) 185
EcoICRI GAGCTC 2 cut(s) 578, 691
EcoO109I RGGNCCY 2 cut(s) 416, 631
EcoRII CCWGG 1 cut(s) 90
EcoT22I ATGCAT 1 cut(s) 241
EcoT38I GRGCYC 2 cut(s) 580, 693
Esp3I CGTCTC 1 cut(s) 441
FaeI CATG 3 cut(s) 120, 215, 697
FaiI YATR 4 cut(s) 118, 213, 681, 695
FaqI GGGAC 4 cut(s) 167, 277, 333, 429
FatI CATG 3 cut(s) 116, 211, 693
FblI GTMKAC 1 cut(s) 725
Fnu4HI GCNGC 5 cut(s) 18, 69, 111, 387, 465
FokI GGATG 2 cut(s) 524, 649
FriOI GRGCYC 2 cut(s) 580, 693
Fsp4HI GCNGC 5 cut(s) 18, 69, 111, 387, 465
FspBI CTAG 3 cut(s) 390, 560, 924
GlaI GCGC 2 cut(s) 367, 715
GluI GCNGC 5 cut(s) 18, 69, 111, 387, 465
HaeIII GGCC 2 cut(s) 161, 481
HapII CCGG 3 cut(s) 186, 281, 351
HhaI GCGC 2 cut(s) 368, 716
Hin1II CATG 3 cut(s) 120, 215, 697
Hin6I GCGC 2 cut(s) 366, 714
HinP1I GCGC 2 cut(s) 366, 714
HincII GTYRAC 1 cut(s) 556
HindII GTYRAC 1 cut(s) 556
HinfI GANTC 4 cut(s) 317, 430, 818, 905
HpaII CCGG 3 cut(s) 186, 281, 351
HphI GGTGA 1 cut(s) 956
Hpy166II GTNNAC 2 cut(s) 556, 726
Hpy188I TCNGA 7 cut(s) 143, 166, 429, 590, 614, 817, 975
Hpy188III TCNNGA 3 cut(s) 573, 892, 913
Hpy8I GTNNAC 2 cut(s) 556, 726
Hpy99I CGWCG 1 cut(s) 979
HpyAV CCTTC 3 cut(s) 31, 132, 944
HpyCH4III ACNGT 4 cut(s) 151, 181, 358, 733
HpyF10VI GCNNNNNNNGC 7 cut(s) 74, 83, 92, 386, 395, 697, 711
HpyF3I CTNAG 5 cut(s) 267, 426, 667, 790, 903
Hsp92II CATG 3 cut(s) 120, 215, 697
HspAI GCGC 2 cut(s) 366, 714
Kzo9I GATC 6 cut(s) 155, 277, 312, 590, 616, 742
LguI GCTCTTC 2 cut(s) 212, 533
LmnI GCTCC 4 cut(s) 198, 575, 688, 697
Lsp1109I GCAGC 5 cut(s) 29, 80, 122, 373, 476
LweI GCATC 4 cut(s) 226, 248, 385, 936
MaeI CTAG 3 cut(s) 390, 560, 924
MalI GATC 6 cut(s) 157, 279, 314, 592, 618, 744
MboI GATC 6 cut(s) 155, 277, 312, 590, 616, 742
MboII GAAGA 7 cut(s) 19, 199, 334, 337, 520, 525, 949
MhlI GDGCHC 3 cut(s) 580, 693, 707
MluCI AATT 5 cut(s) 51, 100, 404, 644, 957
MlyI GAGTC 1 cut(s) 914
MmeI TCCRAC 2 cut(s) 189, 458
MnlI CCTC 7 cut(s) 172, 206, 220, 293, 679, 794, 976
Mph1103I ATGCAT 1 cut(s) 241
MroXI GAANNNNTTC 1 cut(s) 358
MseI TTAA 2 cut(s) 50, 522
MslI CAYNNNNRTG 3 cut(s) 39, 603, 932
MspI CCGG 3 cut(s) 186, 281, 351
MspR9I CCNGG 4 cut(s) 92, 186, 187, 351
Mva1269I GAATGC 1 cut(s) 608
MvaI CCWGG 1 cut(s) 92
MwoI GCNNNNNNNGC 7 cut(s) 74, 83, 92, 386, 395, 697, 711
NciI CCSGG 3 cut(s) 186, 187, 351
NdeII GATC 6 cut(s) 155, 277, 312, 590, 616, 742
NheI GCTAGC 1 cut(s) 923
NlaIII CATG 3 cut(s) 120, 215, 697
NlaIV GGNNCC 3 cut(s) 174, 417, 633
NmeAIII GCCGAG 1 cut(s) 154
NsiI ATGCAT 1 cut(s) 241
OliI CACNNNNGTG 1 cut(s) 603
PceI AGGCCT 1 cut(s) 481
PciSI GCTCTTC 2 cut(s) 212, 533
PctI GAATGC 1 cut(s) 608
PdmI GAANNNNTTC 1 cut(s) 358
PfeI GAWTC 3 cut(s) 317, 430, 818
PflFI GACNNNGTC 1 cut(s) 904
PfoI TCCNGGA 1 cut(s) 349
PkrI GCNGC 5 cut(s) 19, 70, 112, 388, 466
PleI GAGTC 1 cut(s) 913
PpsI GAGTC 1 cut(s) 913
PpuMI RGGWCCY 2 cut(s) 416, 631
Psp124BI GAGCTC 2 cut(s) 580, 693
Psp5II RGGWCCY 2 cut(s) 416, 631
Psp6I CCWGG 1 cut(s) 90
PspGI CCWGG 1 cut(s) 90
PspN4I GGNNCC 3 cut(s) 174, 417, 633
PspPI GGNCC 4 cut(s) 172, 416, 631, 874
PspPPI RGGWCCY 2 cut(s) 416, 631
PstI CTGCAG 4 cut(s) 88, 466, 605, 712
PstNI CAGNNNCTG 1 cut(s) 74
PsyI GACNNNGTC 1 cut(s) 904
RsaI GTAC 1 cut(s) 776
RsaNI GTAC 1 cut(s) 775
RseI CAYNNNNRTG 3 cut(s) 39, 603, 932
SacI GAGCTC 2 cut(s) 580, 693
SapI GCTCTTC 2 cut(s) 212, 533
SaqAI TTAA 2 cut(s) 50, 522
SatI GCNGC 5 cut(s) 18, 69, 111, 387, 465
Sau3AI GATC 6 cut(s) 155, 277, 312, 590, 616, 742
Sau96I GGNCC 4 cut(s) 172, 416, 631, 874
SbfI CCTGCAGG 1 cut(s) 712
ScaI AGTACT 1 cut(s) 776
SchI GAGTC 1 cut(s) 914
ScrFI CCNGG 4 cut(s) 92, 186, 187, 351
SdaI CCTGCAGG 1 cut(s) 712
SduI GDGCHC 3 cut(s) 580, 693, 707
SfaNI GCATC 4 cut(s) 226, 248, 385, 936
SfcI CTRYAG 4 cut(s) 84, 462, 601, 708
SinI GGWCC 4 cut(s) 172, 416, 631, 874
SmaI CCCGGG 1 cut(s) 187
SmiMI CAYNNNNRTG 3 cut(s) 39, 603, 932
Sse8387I CCTGCAGG 1 cut(s) 712
Sse9I AATT 5 cut(s) 51, 100, 404, 644, 957
SseBI AGGCCT 1 cut(s) 481
SspI AATATT 1 cut(s) 487
SspMI CTAG 3 cut(s) 390, 560, 924
SstI GAGCTC 2 cut(s) 580, 693
StuI AGGCCT 1 cut(s) 481
StyD4I CCNGG 4 cut(s) 90, 184, 185, 349
TaaI ACNGT 4 cut(s) 151, 181, 358, 733
TaqI TCGA 3 cut(s) 315, 765, 961
TasI AATT 5 cut(s) 51, 100, 404, 644, 957
TatI WGTACW 1 cut(s) 774
TfiI GAWTC 3 cut(s) 317, 430, 818
Tru1I TTAA 2 cut(s) 50, 522
Tru9I TTAA 2 cut(s) 50, 522
TscAI CASTG 7 cut(s) 88, 102, 156, 315, 605, 610, 707
TseI GCWGC 5 cut(s) 17, 68, 110, 386, 464
TspDTI ATGAA 2 cut(s) 105, 963
TspGWI ACGGA 1 cut(s) 188
TspMI CCCGGG 1 cut(s) 185
TspRI CASTG 7 cut(s) 88, 102, 156, 315, 605, 610, 707
Tth111I GACNNNGTC 1 cut(s) 904
VpaK11BI GGWCC 4 cut(s) 172, 416, 631, 874
XapI RAATTY 4 cut(s) 100, 404, 644, 957
XcmI CCANNNNNNNNNTGG 1 cut(s) 88
XmaI CCCGGG 1 cut(s) 185
XmiI GTMKAC 1 cut(s) 725
XmnI GAANNNNTTC 1 cut(s) 358
XspI CTAG 3 cut(s) 390, 560, 924
ZrmI AGTACT 1 cut(s) 776
Zsp2I ATGCAT 1 cut(s) 241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.