FvH4_3g00010

Protease Do-like 10

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0014559
Physical Location & Seq
Reverse (-)
9890 .. 17350
7461 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g00010.t1

Sequence Viewer

Length: 1389 bp
ATGCTGGTTGCACTTAGAAGGCGGCTATGCTCTTCCGTCTCCTCTATTGAGTTGATCAAAGTTTATACGGCAAGTAGAAGTATTGGCTCTAGTAGTTGTACCGATGTGGCAGCTAATGTTGCACAAGATTGTTCCTCACCTGAGTCTAAAATTGAGAATGGGCCGTTCGCCTCCCCACCCGTCAGGAATATACTCAGACGACGACGTTTAGGTATTCGGAGGAGCAGGAGCACAAGGGATACGGATGCCATAGAGCTGGCATTGGACTCAGTCGTGAAGATTTTTACTGTGGCGAGCAGCCCTAACTACTTCCTGCCCTGGCAGAACAAGTCCCAACGAGAGACCATGGGTTCCGGGTTTGTAATTGCTGGCAACAAAATTCTTACAAATGCTCATGTAGTTGCTGATCATACCTTTGTACTTGTAAGAAAGCATGGCTCTCCTACCAAGCATAAAGCGCAAGTTGAAGCTGTTGGTCATGAATGTGATTTAGCCATTTTAAGTGTTGAAAGTGATAAGTTCTGGGAGGGTATGAATCCCCTTGAGCTAGGAGATATTCCTTTACTGCAAGAAGCCGTTGCTGTTGTTGGATATCCTCAAGGTGGGGACAACATCTCTGTTACTAAGGGCGTTGTTTCCAGGGTTGAACCTACACAATATGTTCATGGTGCAACCCAGCTTATGGCAATACAGATTGATGCAGCAATAAATCCGGGGAATAGTGGTGGCCCAGCAATCATGGGAAGTAAAGTTGCTGGTGTTGCTTTTCAAAACCTTTCTGGTGCAGAGAATATTGGTTATATAATTCCTGTTCCTGTAATAAAGCATTTTATTGCTGGAGTTGAAGAAAGAGGTAAATATGTTGGATTCTGCTCTCTAGGTTTGTCATGCCAGCCTACTGAAAATGTTCAACTCCGGAACCACTTTCGCATGAATCCTGAAATGACTGGGGTACTAGTGAGCAAAATCAACCCTCTTTCGGATGCCCACAAAGTCTTAAAGAAAGATGATATTGTCCTTGTCTTTGATGGAGTCCCTGTAGCCAATGATGGAACAGTTCACTTCAGGAACAGAGAGCGGATAACATTTGATCATTTGGTTTCCATGAAGAAACCCAATGAAACAGCTGTAGTTAGAGTTCTGAGGGATGGCAAAGAATATGAATTCAGTATCACCCTTCAACCTTTACAACCATTAGTTCCAGTTCATCAGTTCGATAAAGTTCCTAGTTATTACATTTTTGCCGGTTTGGTCTTTGTTCCACTCACTCAACCTTACCTTCATGAGTATGGAGAAGACTGGTACTCATGGATGATATCAATGCTGGATATGAACGTCTTGCTGAGCTACAGGTTAAGAAGGTTAATGGGAAAGAAGTTGAAAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PDZ_2 Trypsin_2

Protein Analysis

463

Amino Acids

51.02

Weight (kDa)

8.96

Isoelectric Point (pI)

50.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trypsin PF00089 100 - 278 3.5e-20 Trypsin
Trypsin_2 PF13365 117 - 246 3.8e-22 Trypsin-like peptidase domain
PDZ_2 PF13180 293 - 393 1.9e-06 PDZ domain
PDZ_3 PF17815 400 - 436 1.4e-13 PDZ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014559)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G36950
fragaria_vesca FvH4_3g00010
malus_domestica MD08G1000100.v1.1
prunus_persica Prupe.4G000300_v2.0.a1
pyrus_communis pycom08g00010
rosa_chinensis RchiOBHm_Chr5g0000001
rosa_laevigata RLG00000030831
rosa_multiflora Rmu_sc0007644.1_g000006
rosa_roxburghii Rroxscaffold_1G00076060 Rroxscaffold_2G00109790 Rroxscaffold_4G00330340
rosa_rugosa Rorug04G0380000
rosa_samantha Rh5AG000100 Rh5BG001300 Rh5CG000100 Rh5DG000100
rosa_wichuraiana Rw5G000040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 682
AccBSI CCGCTC 1 cut(s) 1078
AccIII TCCGGA 1 cut(s) 915
AciI CCGC 2 cut(s) 22, 1078
AcsI RAATTY 3 cut(s) 378, 1163, 1383
AcuI CTGAAG 1 cut(s) 1048
AfaI GTAC 4 cut(s) 100, 420, 954, 1304
AfiI CCNNNNNNNGG 3 cut(s) 602, 682, 979
AgsI TTSAA 8 cut(s) 467, 509, 647, 770, 845, 911, 1181, 1381
AhlI ACTAGT 1 cut(s) 955
AjnI CCWGG 2 cut(s) 317, 638
AluBI AGCT 7 cut(s) 113, 256, 470, 547, 679, 1127, 1347
AluI AGCT 7 cut(s) 113, 256, 470, 547, 679, 1127, 1347
Alw21I GWGCWC 1 cut(s) 233
Alw26I GTCTC 2 cut(s) 43, 335
Aor13HI TCCGGA 1 cut(s) 915
AoxI GGCC 2 cut(s) 161, 727
ApeKI GCWGC 3 cut(s) 110, 297, 701
ApoI RAATTY 3 cut(s) 378, 1163, 1383
Asp700I GAANNNNTTC 1 cut(s) 906
AspLEI GCGC 1 cut(s) 460
AspS9I GGNCC 2 cut(s) 161, 728
AsuC2I CCSGG 2 cut(s) 355, 714
AsuHPI GGTGA 2 cut(s) 129, 1165
BbsI GAAGAC 1 cut(s) 1302
Bbv12I GWGCWC 1 cut(s) 233
BbvI GCAGC 3 cut(s) 122, 309, 713
BccI CCATC 3 cut(s) 1022, 1043, 1142
BceAI ACGGC 3 cut(s) 84, 148, 560
BciT130I CCWGG 2 cut(s) 319, 640
BciVI GTATCC 1 cut(s) 232
BclI TGATCA 3 cut(s) 54, 406, 1090
BcnI CCSGG 2 cut(s) 355, 714
BcoDI GTCTC 2 cut(s) 43, 335
BcuI ACTAGT 1 cut(s) 955
BfaI CTAG 5 cut(s) 90, 548, 878, 956, 1227
BfmI CTRYAG 3 cut(s) 1038, 1128, 1348
BfuI GTATCC 1 cut(s) 232
BisI GCNGC 4 cut(s) 23, 111, 298, 702
BlpI GCTNAGC 1 cut(s) 1343
BlsI GCNGC 4 cut(s) 24, 112, 299, 703
Bme1390I CCNGG 4 cut(s) 319, 355, 640, 714
BmgT120I GGNCC 2 cut(s) 161, 728
BmiI GGNNCC 2 cut(s) 352, 920
BmrFI CCNGG 4 cut(s) 319, 355, 640, 714
BmrI ACTGGG 1 cut(s) 957
BmsI GCATC 3 cut(s) 235, 688, 973
BmuI ACTGGG 1 cut(s) 957
BpiI GAAGAC 1 cut(s) 1302
BpmI CTGGAG 1 cut(s) 858
Bpu1102I GCTNAGC 1 cut(s) 1343
BpuEI CTTGAG 2 cut(s) 563, 582
BpuMI CCSGG 2 cut(s) 355, 714
BsaI GGTCTC 1 cut(s) 335
BsaJI CCNNGG 4 cut(s) 317, 345, 639, 713
BsaWI WCCGGW 1 cut(s) 915
BsaXI ACNNNNNCTCC 2 cut(s) 1023, 1053
Bsc4I CCNNNNNNNGG 3 cut(s) 602, 682, 979
Bse118I RCCGGY 1 cut(s) 1244
Bse1I ACTGG 3 cut(s) 952, 1202, 1304
BseAI TCCGGA 1 cut(s) 915
BseBI CCWGG 2 cut(s) 319, 640
BseDI CCNNGG 4 cut(s) 317, 345, 639, 713
BseGI GGATG 4 cut(s) 250, 988, 1153, 1317
BseLI CCNNNNNNNGG 3 cut(s) 602, 682, 979
BseMII CTCAG 5 cut(s) 132, 208, 282, 1133, 1334
BseNI ACTGG 3 cut(s) 952, 1202, 1304
BseRI GAGGAG 2 cut(s) 31, 235
BseXI GCAGC 3 cut(s) 122, 309, 713
BseYI CCCAGC 2 cut(s) 675, 730
BsgI GTGCAG 1 cut(s) 804
BshFI GGCC 2 cut(s) 163, 729
BsiHKAI GWGCWC 1 cut(s) 233
BsiSI CCGG 4 cut(s) 354, 713, 916, 1245
BslFI GGGAC 3 cut(s) 316, 620, 1019
BslI CCNNNNNNNGG 3 cut(s) 602, 682, 979
BsmAI GTCTC 2 cut(s) 43, 335
BsmBI CGTCTC 1 cut(s) 43
BsmFI GGGAC 3 cut(s) 316, 620, 1019
BsnI GGCC 2 cut(s) 163, 729
Bso31I GGTCTC 1 cut(s) 335
Bsp1286I GDGCHC 1 cut(s) 233
Bsp13I TCCGGA 1 cut(s) 915
Bsp143I GATC 3 cut(s) 54, 406, 1090
Bsp1720I GCTNAGC 1 cut(s) 1343
Bsp19I CCATGG 1 cut(s) 345
BspACI CCGC 2 cut(s) 22, 1078
BspANI GGCC 2 cut(s) 163, 729
BspCNI CTCAG 5 cut(s) 133, 207, 281, 1134, 1335
BspEI TCCGGA 1 cut(s) 915
BspHI TCATGA 2 cut(s) 478, 1282
BspLI GGNNCC 2 cut(s) 352, 920
BspQI GCTCTTC 1 cut(s) 37
BspTNI GGTCTC 1 cut(s) 335
BsrBI CCGCTC 1 cut(s) 1078
BsrFI RCCGGY 1 cut(s) 1244
BsrI ACTGG 3 cut(s) 952, 1202, 1304
BssAI RCCGGY 1 cut(s) 1244
BssECI CCNNGG 4 cut(s) 317, 345, 639, 713
BssMI GATC 3 cut(s) 54, 406, 1090
BssT1I CCWWGG 1 cut(s) 345
Bst2UI CCWGG 2 cut(s) 319, 640
Bst4CI ACNGT 2 cut(s) 289, 1057
Bst6I CTCTTC 1 cut(s) 37
BstC8I GCNNGC 4 cut(s) 258, 295, 370, 893
BstDEI CTNAG 7 cut(s) 14, 141, 194, 268, 624, 1142, 1343
BstDSI CCRYGG 1 cut(s) 345
BstF5I GGATG 4 cut(s) 250, 988, 1153, 1317
BstHHI GCGC 1 cut(s) 460
BstKTI GATC 3 cut(s) 57, 409, 1093
BstMAI GTCTC 2 cut(s) 43, 335
BstMBI GATC 3 cut(s) 54, 406, 1090
BstMWI GCNNNNNNNGC 3 cut(s) 119, 457, 761
BstNI CCWGG 2 cut(s) 319, 640
BstSCI CCNGG 4 cut(s) 317, 353, 638, 712
BstSFI CTRYAG 3 cut(s) 1038, 1128, 1348
BstV1I GCAGC 3 cut(s) 122, 309, 713
BstV2I GAAGAC 1 cut(s) 1302
BstXI CCANNNNNNTGG 1 cut(s) 256
BsuI GTATCC 1 cut(s) 232
BsuRI GGCC 2 cut(s) 163, 729
BtgI CCRYGG 1 cut(s) 345
BtsCI GGATG 4 cut(s) 250, 988, 1153, 1317
Cac8I GCNNGC 4 cut(s) 258, 295, 370, 893
CciI TCATGA 2 cut(s) 478, 1282
CfoI GCGC 1 cut(s) 460
Cfr10I RCCGGY 1 cut(s) 1244
Cfr13I GGNCC 2 cut(s) 161, 728
Csp6I GTAC 4 cut(s) 99, 419, 953, 1303
CviQI GTAC 4 cut(s) 99, 419, 953, 1303
DdeI CTNAG 7 cut(s) 14, 141, 194, 268, 624, 1142, 1343
DpnI GATC 3 cut(s) 56, 408, 1092
DpnII GATC 3 cut(s) 54, 406, 1090
Eam1104I CTCTTC 1 cut(s) 37
EarI CTCTTC 1 cut(s) 37
Eco130I CCWWGG 1 cut(s) 345
Eco31I GGTCTC 1 cut(s) 335
Eco32I GATATC 2 cut(s) 593, 1317
Eco57I CTGAAG 1 cut(s) 1048
EcoRI GAATTC 1 cut(s) 1163
EcoRII CCWGG 2 cut(s) 317, 638
EcoRV GATATC 2 cut(s) 593, 1317
EcoT14I CCWWGG 1 cut(s) 345
ErhI CCWWGG 1 cut(s) 345
Esp3I CGTCTC 1 cut(s) 43
FaqI GGGAC 3 cut(s) 316, 620, 1019
FbaI TGATCA 3 cut(s) 54, 406, 1090
Fnu4HI GCNGC 4 cut(s) 23, 111, 298, 702
FokI GGATG 4 cut(s) 257, 995, 1160, 1324
Fsp4HI GCNGC 4 cut(s) 23, 111, 298, 702
FspBI CTAG 5 cut(s) 90, 548, 878, 956, 1227
GlaI GCGC 1 cut(s) 459
GluI GCNGC 4 cut(s) 23, 111, 298, 702
GsaI CCCAGC 2 cut(s) 679, 734
GsuI CTGGAG 1 cut(s) 858
HaeIII GGCC 2 cut(s) 163, 729
HapII CCGG 4 cut(s) 354, 713, 916, 1245
HhaI GCGC 1 cut(s) 460
Hin6I GCGC 1 cut(s) 458
HinP1I GCGC 1 cut(s) 458
HinfI GANTC 6 cut(s) 143, 266, 535, 867, 934, 1032
HpaII CCGG 4 cut(s) 354, 713, 916, 1245
HphI GGTGA 2 cut(s) 129, 1165
Hpy166II GTNNAC 1 cut(s) 1060
Hpy188I TCNGA 4 cut(s) 197, 219, 982, 1143
Hpy188III TCNNGA 7 cut(s) 184, 274, 479, 916, 938, 1066, 1283
Hpy8I GTNNAC 1 cut(s) 1060
Hpy99I CGWCG 2 cut(s) 204, 207
HpyAV CCTTC 4 cut(s) 12, 1187, 1289, 1353
HpyCH4III ACNGT 2 cut(s) 289, 1057
HpyCH4IV ACGT 2 cut(s) 205, 1335
HpyCH4V TGCA 6 cut(s) 11, 122, 568, 671, 701, 785
HpyF10VI GCNNNNNNNGC 3 cut(s) 119, 457, 761
HpyF3I CTNAG 7 cut(s) 14, 141, 194, 268, 624, 1142, 1343
HpySE526I ACGT 2 cut(s) 205, 1335
HspAI GCGC 1 cut(s) 458
Kpn2I TCCGGA 1 cut(s) 915
Ksp22I TGATCA 3 cut(s) 54, 406, 1090
Kzo9I GATC 3 cut(s) 54, 406, 1090
LguI GCTCTTC 1 cut(s) 37
LmnI GCTCC 2 cut(s) 222, 228
Lsp1109I GCAGC 3 cut(s) 122, 309, 713
LweI GCATC 3 cut(s) 235, 688, 973
MaeI CTAG 5 cut(s) 90, 548, 878, 956, 1227
MaeII ACGT 2 cut(s) 205, 1335
MaeIII GTNAC 1 cut(s) 619
MalI GATC 3 cut(s) 56, 408, 1092
MbiI CCGCTC 1 cut(s) 1078
MboI GATC 3 cut(s) 54, 406, 1090
MboII GAAGA 5 cut(s) 24, 289, 857, 1120, 1307
MhlI GDGCHC 1 cut(s) 233
MluCI AATT 6 cut(s) 150, 363, 378, 804, 1163, 1383
MlyI GAGTC 3 cut(s) 152, 260, 1041
MmeI TCCRAC 2 cut(s) 568, 844
MnlI CCTC 9 cut(s) 52, 145, 181, 213, 520, 606, 845, 984, 1137
MroI TCCGGA 1 cut(s) 915
MroXI GAANNNNTTC 1 cut(s) 906
MseI TTAA 4 cut(s) 500, 998, 1355, 1364
MslI CAYNNNNRTG 2 cut(s) 483, 1287
MspA1I CMGCKG 1 cut(s) 1127
MspI CCGG 4 cut(s) 354, 713, 916, 1245
MspR9I CCNGG 4 cut(s) 319, 355, 640, 714
MvaI CCWGG 2 cut(s) 319, 640
MwoI GCNNNNNNNGC 3 cut(s) 119, 457, 761
NciI CCSGG 2 cut(s) 355, 714
NcoI CCATGG 1 cut(s) 345
NdeII GATC 3 cut(s) 54, 406, 1090
NlaIV GGNNCC 2 cut(s) 352, 920
PagI TCATGA 2 cut(s) 478, 1282
PciSI GCTCTTC 1 cut(s) 37
PdmI GAANNNNTTC 1 cut(s) 906
PfeI GAWTC 3 cut(s) 535, 867, 934
PflFI GACNNNGTC 1 cut(s) 269
PflMI CCANNNNNTGG 1 cut(s) 682
PkrI GCNGC 4 cut(s) 24, 112, 299, 703
PleI GAGTC 3 cut(s) 151, 260, 1040
PpsI GAGTC 3 cut(s) 151, 260, 1040
Psp6I CCWGG 2 cut(s) 317, 638
PspFI CCCAGC 2 cut(s) 675, 730
PspGI CCWGG 2 cut(s) 317, 638
PspN4I GGNNCC 2 cut(s) 352, 920
PspPI GGNCC 2 cut(s) 161, 728
PsrI GAACNNNNNNTAC 2 cut(s) 1122, 1154
PsyI GACNNNGTC 1 cut(s) 269
PvuII CAGCTG 1 cut(s) 1127
RsaI GTAC 4 cut(s) 100, 420, 954, 1304
RsaNI GTAC 4 cut(s) 99, 419, 953, 1303
RseI CAYNNNNRTG 2 cut(s) 483, 1287
SapI GCTCTTC 1 cut(s) 37
SaqAI TTAA 4 cut(s) 500, 998, 1355, 1364
SatI GCNGC 4 cut(s) 23, 111, 298, 702
Sau3AI GATC 3 cut(s) 54, 406, 1090
Sau96I GGNCC 2 cut(s) 161, 728
SchI GAGTC 3 cut(s) 152, 260, 1041
ScrFI CCNGG 4 cut(s) 319, 355, 640, 714
SduI GDGCHC 1 cut(s) 233
SfaNI GCATC 3 cut(s) 235, 688, 973
SfcI CTRYAG 3 cut(s) 1038, 1128, 1348
SmiMI CAYNNNNRTG 2 cut(s) 483, 1287
SmlI CTYRAG 2 cut(s) 542, 597
SmoI CTYRAG 2 cut(s) 542, 597
SpeI ACTAGT 1 cut(s) 955
Sse9I AATT 6 cut(s) 150, 363, 378, 804, 1163, 1383
SsiI CCGC 2 cut(s) 22, 1078
SspI AATATT 1 cut(s) 793
SspMI CTAG 5 cut(s) 90, 548, 878, 956, 1227
StyD4I CCNGG 4 cut(s) 317, 353, 638, 712
StyI CCWWGG 1 cut(s) 345
TaaI ACNGT 2 cut(s) 289, 1057
TaiI ACGT 2 cut(s) 208, 1338
TaqI TCGA 1 cut(s) 1215
TasI AATT 6 cut(s) 150, 363, 378, 804, 1163, 1383
TatI WGTACW 1 cut(s) 418
TauI GCSGC 1 cut(s) 25
TfiI GAWTC 3 cut(s) 535, 867, 934
Tru1I TTAA 4 cut(s) 500, 998, 1355, 1364
Tru9I TTAA 4 cut(s) 500, 998, 1355, 1364
TseI GCWGC 3 cut(s) 110, 297, 701
TspGWI ACGGA 2 cut(s) 25, 257
Tth111I GACNNNGTC 1 cut(s) 269
Van91I CCANNNNNTGG 1 cut(s) 682
XapI RAATTY 3 cut(s) 378, 1163, 1383
XmnI GAANNNNTTC 1 cut(s) 906
XspI CTAG 5 cut(s) 90, 548, 878, 956, 1227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.