Rroxscaffold_1G00076060

Protease Do-like 10

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0014559
Physical Location & Seq
Forward (+)
96608336 .. 96615034
6699 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00076060.1

Sequence Viewer

Length: 1671 bp
ATGCTGGTAACGTCCGCCCGAACACTCCTCAGAAGGCGGCTATGCTCTATTGAGTTGTTAAAAGATTATACGTCAAGGAGAAGTGGTATTAGCTGTAGTAGTAGTACCTGGGCGGCAGCTAATGTTGAAGAAGATGCTGCTGCTGCTGCTTCTTGTTCCTCGTCTGAGTCGGTGTCGGTCGCCTCCCCCTCAGTCAGGAATAATATGCTCCGACGACGACGTTTCGGTATTCGGAGGAACAACGAGAGCCGGAGCACAAAGGATACGGATGCCGTAGAGCTGGCATTGGACTCAGTGGTGAAGATCTTTACTGTGGCGAGCAGCCCTAACTACTTCCTGCCCTGGCAGAACAAGTCCCAGCGAGAGACCATGGGTTCCGGATTTGTGATCGCCGGCAAGAAAATTCTTACAAACGCTCATGTAGTTGCTGATCATACATTTGTTCTAGTAAGAAAGCATGGCTCTCCTACCAAGTATAAAGCGCAAGTTGAAGCTGTTGGTCATGAATGTGATTTAGCCATTTTGAGTGTTGAAACTGAAAAGTTCTGGGAGGGTATGAATTCCCTTGAGCTAGGGGATATTCCTTTTCTGCAAGAAGCTGTTGCTGTTGTTGGATATCCTCAAGGTGGGGACAATATCTCTGTTACTAAAGGTGTTGTTTCCAGGGTTGAACCTACACAATATGTTCATGGTGCAACCCAGCTTATGGCAATACAGATTGATGCAGCAATAAATCCCGGAAATAGTGGTGGCCCAGCAATCATGGGAAATAAAGTTGCTGGTGTTGCTTTCCAAAACCTTTCTGGTGCAGAGAATATTGGTTATATAATTCCTGTTCCTGTAATAAAGCATTTTATTGCTGGAGTTGAAGAAAGAGGTAAATATGTTGGATTCTGCTCTCTAGGTTTGTCATGCCAACCTACTGAAAATGTTCAACTCCGGAACCACTTTCAAATGCGTCCTGAAATGACTGGGGTACTAGTGAGCAAAATCAACCCTCTTTCGGATGCCCACAAAGTCTTAAAGAAAGATGATGTTGTTCTTTTCTTTGATGGAGTTCCTGTAGCAAATGATGGAACAGTTCACTTCAGGAACAGAGAGCGGATAACATTTGATCATTTGGTGTCCATGAAGAAACCCAATGAAACAGCTGTAGTCAGAGTTTTGAGGGATGGTCAAGAATATGAATTCAGTATCACCCTTCAACCTTTACAACCGTTAGTTCCAGTTCAGCAGTTCGATAAACTTCCTAGTTATTACATCTTTGCTGGTTTGGTCTTTGTTCCGCTCACTCAACCTTACCTTCATGAGTATGGAGAAGATTGGTATAATACTTCACCTCGTAAATTATGCGAAATCGCATTGAGGGAACCACCTAAAAGAGCTGGTGAACAGATTGTCATCCTTTCTCAGGTACTCATGGATGATATCAATGCTGGATATGAACGTCTGGCAGAGCTGCAGGTTAAGAAGGTTAACGGGATAGAAGTTGAAAATTTGAAACATTTATGCCAACTTGTCGAAAACTGTAGTGAAGAGAGCGTGCGTTTTGATTTGGATGATGATAGAGTTGTTGTGTTGAACTATCATTTAGCAAAAGTTGCGACTTCAAAAATTCTAAATTGCCACAGGATACCCACTGCAATGTCTAATGATCTTTTAGAGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PDZ_2 Trypsin_2

Protein Analysis

556

Amino Acids

61.6

Weight (kDa)

6.72

Isoelectric Point (pI)

46.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trypsin PF00089 108 - 286 3.9e-18 Trypsin
Trypsin_2 PF13365 125 - 254 6e-21 Trypsin-like peptidase domain
PDZ_2 PF13180 318 - 401 6.3e-06 PDZ domain
PDZ_3 PF17815 408 - 552 5.1e-55 PDZ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014559)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G36950
fragaria_vesca FvH4_3g00010
malus_domestica MD08G1000100.v1.1
prunus_persica Prupe.4G000300_v2.0.a1
pyrus_communis pycom08g00010
rosa_chinensis RchiOBHm_Chr5g0000001
rosa_laevigata RLG00000030831
rosa_multiflora Rmu_sc0007644.1_g000006
rosa_roxburghii Rroxscaffold_1G00076060 Rroxscaffold_2G00109790 Rroxscaffold_4G00330340
rosa_rugosa Rorug04G0380000
rosa_samantha Rh5AG000100 Rh5BG001300 Rh5CG000100 Rh5DG000100
rosa_wichuraiana Rw5G000040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1453
AccB7I CCANNNNNTGG 1 cut(s) 706
AccBSI CCGCTC 2 cut(s) 1102, 1288
AccIII TCCGGA 2 cut(s) 377, 939
AciI CCGC 5 cut(s) 15, 37, 113, 1102, 1286
AcsI RAATTY 5 cut(s) 402, 559, 1187, 1495, 1614
AcuI CTGAAG 1 cut(s) 1072
AfaI GTAC 3 cut(s) 106, 978, 1416
AfiI CCNNNNNNNGG 5 cut(s) 195, 626, 706, 1003, 1411
AhlI ACTAGT 1 cut(s) 979
AjnI CCWGG 3 cut(s) 107, 341, 662
Alw21I GWGCWC 1 cut(s) 257
Alw26I GTCTC 1 cut(s) 359
Aor13HI TCCGGA 2 cut(s) 377, 939
AoxI GGCC 1 cut(s) 751
ApeKI GCWGC 8 cut(s) 116, 137, 140, 143, 146, 321, 725, 1459
ApoI RAATTY 5 cut(s) 402, 559, 1187, 1495, 1614
Asp700I GAANNNNTTC 1 cut(s) 930
AspLEI GCGC 1 cut(s) 484
AspS9I GGNCC 1 cut(s) 752
AsuC2I CCSGG 1 cut(s) 738
AsuHPI GGTGA 4 cut(s) 310, 1189, 1329, 1400
Bbv12I GWGCWC 1 cut(s) 257
BbvI GCAGC 8 cut(s) 124, 127, 128, 130, 133, 333, 737, 1446
BccI CCATC 3 cut(s) 1046, 1067, 1166
BceAI ACGGC 1 cut(s) 257
BciT130I CCWGG 3 cut(s) 109, 343, 664
BciVI GTATCC 2 cut(s) 256, 1626
BclI TGATCA 2 cut(s) 430, 1114
BcnI CCSGG 1 cut(s) 738
BcoDI GTCTC 1 cut(s) 359
BcuI ACTAGT 1 cut(s) 979
BfaI CTAG 5 cut(s) 446, 572, 902, 980, 1251
BfmI CTRYAG 5 cut(s) 94, 1062, 1152, 1460, 1528
BfuAI ACCTGC 1 cut(s) 1453
BfuI GTATCC 2 cut(s) 256, 1626
BglII AGATCT 1 cut(s) 303
Bme1390I CCNGG 4 cut(s) 109, 343, 664, 738
BmgT120I GGNCC 1 cut(s) 752
BmiI GGNNCC 3 cut(s) 376, 944, 1371
BmrFI CCNGG 4 cut(s) 109, 343, 664, 738
BmrI ACTGGG 1 cut(s) 981
BmsI GCATC 4 cut(s) 124, 259, 712, 997
BmuI ACTGGG 1 cut(s) 981
BpmI CTGGAG 1 cut(s) 882
BpuEI CTTGAG 2 cut(s) 587, 606
BpuMI CCSGG 1 cut(s) 738
BsaBI GATNNNNATC 1 cut(s) 1400
BsaI GGTCTC 1 cut(s) 359
BsaJI CCNNGG 4 cut(s) 108, 341, 369, 663
BsaWI WCCGGW 2 cut(s) 377, 939
BsaXI ACNNNNNCTCC 4 cut(s) 70, 100, 1047, 1077
Bsc4I CCNNNNNNNGG 5 cut(s) 195, 626, 706, 1003, 1411
Bse118I RCCGGY 1 cut(s) 392
Bse1I ACTGG 2 cut(s) 976, 1226
Bse3DI GCAATG 1 cut(s) 1650
Bse8I GATNNNNATC 1 cut(s) 1400
BseAI TCCGGA 2 cut(s) 377, 939
BseBI CCWGG 3 cut(s) 109, 343, 664
BseDI CCNNGG 4 cut(s) 108, 341, 369, 663
BseGI GGATG 6 cut(s) 274, 1012, 1177, 1401, 1429, 1564
BseJI GATNNNNATC 1 cut(s) 1400
BseLI CCNNNNNNNGG 5 cut(s) 195, 626, 706, 1003, 1411
BseMI GCAATG 1 cut(s) 1650
BseMII CTCAG 5 cut(s) 43, 156, 204, 306, 1424
BseNI ACTGG 2 cut(s) 976, 1226
BseRI GAGGAG 1 cut(s) 17
BseXI GCAGC 8 cut(s) 124, 127, 128, 130, 133, 333, 737, 1446
BseYI CCCAGC 3 cut(s) 357, 699, 754
BsgI GTGCAG 1 cut(s) 828
Bsh1285I CGRYCG 1 cut(s) 180
BshFI GGCC 1 cut(s) 753
BsiEI CGRYCG 1 cut(s) 180
BsiHKAI GWGCWC 1 cut(s) 257
BsiSI CCGG 5 cut(s) 250, 378, 393, 738, 940
BslFI GGGAC 2 cut(s) 340, 644
BslI CCNNNNNNNGG 5 cut(s) 195, 626, 706, 1003, 1411
BsmAI GTCTC 1 cut(s) 359
BsmFI GGGAC 2 cut(s) 340, 644
BsnI GGCC 1 cut(s) 753
Bso31I GGTCTC 1 cut(s) 359
Bsp1286I GDGCHC 1 cut(s) 257
Bsp13I TCCGGA 2 cut(s) 377, 939
Bsp143I GATC 5 cut(s) 303, 387, 430, 1114, 1654
Bsp19I CCATGG 1 cut(s) 369
BspACI CCGC 5 cut(s) 15, 37, 113, 1102, 1286
BspANI GGCC 1 cut(s) 753
BspCNI CTCAG 5 cut(s) 42, 157, 203, 305, 1423
BspEI TCCGGA 2 cut(s) 377, 939
BspHI TCATGA 2 cut(s) 502, 1306
BspLI GGNNCC 3 cut(s) 376, 944, 1371
BspMAI CTGCAG 1 cut(s) 1464
BspMI ACCTGC 1 cut(s) 1453
BspTNI GGTCTC 1 cut(s) 359
BsrBI CCGCTC 2 cut(s) 1102, 1288
BsrDI GCAATG 1 cut(s) 1650
BsrFI RCCGGY 1 cut(s) 392
BsrI ACTGG 2 cut(s) 976, 1226
BssAI RCCGGY 1 cut(s) 392
BssECI CCNNGG 4 cut(s) 108, 341, 369, 663
BssMI GATC 5 cut(s) 303, 387, 430, 1114, 1654
BssT1I CCWWGG 1 cut(s) 369
Bst2UI CCWGG 3 cut(s) 109, 343, 664
Bst4CI ACNGT 4 cut(s) 313, 1081, 1218, 1529
Bst6I CTCTTC 1 cut(s) 1530
BstAPI GCANNNNNTGC 1 cut(s) 1601
BstC8I GCNNGC 4 cut(s) 282, 319, 394, 1544
BstDEI CTNAG 5 cut(s) 29, 165, 190, 292, 1410
BstDSI CCRYGG 1 cut(s) 369
BstENI CCTNNNNNAGG 2 cut(s) 193, 1409
BstF5I GGATG 6 cut(s) 274, 1012, 1177, 1401, 1429, 1564
BstHHI GCGC 1 cut(s) 484
BstKTI GATC 5 cut(s) 306, 390, 433, 1117, 1657
BstMAI GTCTC 1 cut(s) 359
BstMBI GATC 5 cut(s) 303, 387, 430, 1114, 1654
BstMCI CGRYCG 1 cut(s) 180
BstMWI GCNNNNNNNGC 4 cut(s) 143, 146, 785, 1601
BstNI CCWGG 3 cut(s) 109, 343, 664
BstSCI CCNGG 4 cut(s) 107, 341, 662, 736
BstSFI CTRYAG 5 cut(s) 94, 1062, 1152, 1460, 1528
BstV1I GCAGC 8 cut(s) 124, 127, 128, 130, 133, 333, 737, 1446
BstX2I RGATCY 1 cut(s) 303
BstYI RGATCY 1 cut(s) 303
BsuI GTATCC 2 cut(s) 256, 1626
BsuRI GGCC 1 cut(s) 753
BtgI CCRYGG 1 cut(s) 369
BtsCI GGATG 6 cut(s) 274, 1012, 1177, 1401, 1429, 1564
BtsI GCAGTG 1 cut(s) 1638
BtsIMutI CAGTG 2 cut(s) 300, 1638
BveI ACCTGC 1 cut(s) 1453
Cac8I GCNNGC 4 cut(s) 282, 319, 394, 1544
CciI TCATGA 2 cut(s) 502, 1306
CfoI GCGC 1 cut(s) 484
Cfr10I RCCGGY 1 cut(s) 392
Cfr13I GGNCC 1 cut(s) 752
CseI GACGC 1 cut(s) 947
Csp6I GTAC 3 cut(s) 105, 977, 1415
CviQI GTAC 3 cut(s) 105, 977, 1415
DdeI CTNAG 5 cut(s) 29, 165, 190, 292, 1410
DpnI GATC 5 cut(s) 305, 389, 432, 1116, 1656
DpnII GATC 5 cut(s) 303, 387, 430, 1114, 1654
Eam1104I CTCTTC 1 cut(s) 1530
EarI CTCTTC 1 cut(s) 1530
EciI GGCGGA 1 cut(s) 4
Eco130I CCWWGG 1 cut(s) 369
Eco31I GGTCTC 1 cut(s) 359
Eco32I GATATC 2 cut(s) 617, 1429
Eco57I CTGAAG 1 cut(s) 1072
EcoNI CCTNNNNNAGG 2 cut(s) 193, 1409
EcoRI GAATTC 2 cut(s) 559, 1187
EcoRII CCWGG 3 cut(s) 107, 341, 662
EcoRV GATATC 2 cut(s) 617, 1429
EcoT14I CCWWGG 1 cut(s) 369
ErhI CCWWGG 1 cut(s) 369
FaqI GGGAC 2 cut(s) 340, 644
FbaI TGATCA 2 cut(s) 430, 1114
FokI GGATG 6 cut(s) 281, 1019, 1184, 1388, 1436, 1571
FspBI CTAG 5 cut(s) 446, 572, 902, 980, 1251
GlaI GCGC 1 cut(s) 483
GsaI CCCAGC 3 cut(s) 361, 703, 758
GsuI CTGGAG 1 cut(s) 882
HaeIII GGCC 1 cut(s) 753
HapII CCGG 5 cut(s) 250, 378, 393, 738, 940
HgaI GACGC 1 cut(s) 947
HhaI GCGC 1 cut(s) 484
Hin6I GCGC 1 cut(s) 482
HinP1I GCGC 1 cut(s) 482
HincII GTYRAC 1 cut(s) 1477
HindII GTYRAC 1 cut(s) 1477
HinfI GANTC 3 cut(s) 167, 290, 891
HpaI GTTAAC 1 cut(s) 1477
HpaII CCGG 5 cut(s) 250, 378, 393, 738, 940
HphI GGTGA 4 cut(s) 310, 1189, 1329, 1400
Hpy166II GTNNAC 3 cut(s) 1084, 1391, 1477
Hpy188I TCNGA 6 cut(s) 32, 166, 212, 234, 1006, 1160
Hpy188III TCNNGA 8 cut(s) 196, 378, 503, 940, 962, 1090, 1178, 1307
Hpy8I GTNNAC 3 cut(s) 1084, 1391, 1477
Hpy99I CGWCG 3 cut(s) 216, 219, 222
HpyAV CCTTC 4 cut(s) 27, 1211, 1313, 1465
HpyCH4III ACNGT 4 cut(s) 313, 1081, 1218, 1529
HpyCH4IV ACGT 4 cut(s) 11, 71, 220, 1447
HpyCH4V TGCA 6 cut(s) 592, 695, 725, 809, 1462, 1643
HpyF10VI GCNNNNNNNGC 4 cut(s) 143, 146, 785, 1601
HpyF3I CTNAG 5 cut(s) 29, 165, 190, 292, 1410
HpySE526I ACGT 4 cut(s) 11, 71, 220, 1447
HspAI GCGC 1 cut(s) 482
Kpn2I TCCGGA 2 cut(s) 377, 939
KroI GCCGGC 1 cut(s) 392
KroNI GCCGGC 1 cut(s) 394
Ksp22I TGATCA 2 cut(s) 430, 1114
KspAI GTTAAC 1 cut(s) 1477
Kzo9I GATC 5 cut(s) 303, 387, 430, 1114, 1654
LmnI GCTCC 2 cut(s) 213, 252
Lsp1109I GCAGC 8 cut(s) 124, 127, 128, 130, 133, 333, 737, 1446
LweI GCATC 4 cut(s) 124, 259, 712, 997
MaeI CTAG 5 cut(s) 446, 572, 902, 980, 1251
MaeII ACGT 4 cut(s) 11, 71, 220, 1447
MaeIII GTNAC 2 cut(s) 7, 643
MalI GATC 5 cut(s) 305, 389, 432, 1116, 1656
MbiI CCGCTC 2 cut(s) 1102, 1288
MboI GATC 5 cut(s) 303, 387, 430, 1114, 1654
MboII GAAGA 7 cut(s) 140, 143, 313, 881, 1144, 1331, 1547
MflI RGATCY 1 cut(s) 303
MhlI GDGCHC 1 cut(s) 257
MluCI AATT 8 cut(s) 402, 559, 828, 1187, 1346, 1495, 1614, 1621
MlyI GAGTC 2 cut(s) 176, 284
MmeI TCCRAC 3 cut(s) 235, 592, 868
MroI TCCGGA 2 cut(s) 377, 939
MroNI GCCGGC 1 cut(s) 392
MroXI GAANNNNTTC 1 cut(s) 930
MseI TTAA 4 cut(s) 59, 1022, 1467, 1476
MslI CAYNNNNRTG 3 cut(s) 507, 1311, 1643
MspA1I CMGCKG 1 cut(s) 1151
MspI CCGG 5 cut(s) 250, 378, 393, 738, 940
MspR9I CCNGG 4 cut(s) 109, 343, 664, 738
MvaI CCWGG 3 cut(s) 109, 343, 664
MwoI GCNNNNNNNGC 4 cut(s) 143, 146, 785, 1601
NaeI GCCGGC 1 cut(s) 394
NciI CCSGG 1 cut(s) 738
NcoI CCATGG 1 cut(s) 369
NdeII GATC 5 cut(s) 303, 387, 430, 1114, 1654
NgoMIV GCCGGC 1 cut(s) 392
NlaIV GGNNCC 3 cut(s) 376, 944, 1371
PagI TCATGA 2 cut(s) 502, 1306
PdiI GCCGGC 1 cut(s) 394
PdmI GAANNNNTTC 1 cut(s) 930
PfeI GAWTC 1 cut(s) 891
PflMI CCANNNNNTGG 1 cut(s) 706
PfoI TCCNGGA 1 cut(s) 736
PleI GAGTC 2 cut(s) 175, 284
PpsI GAGTC 2 cut(s) 175, 284
Psp6I CCWGG 3 cut(s) 107, 341, 662
PspFI CCCAGC 3 cut(s) 357, 699, 754
PspGI CCWGG 3 cut(s) 107, 341, 662
PspN4I GGNNCC 3 cut(s) 376, 944, 1371
PspPI GGNCC 1 cut(s) 752
PstI CTGCAG 1 cut(s) 1464
PsuI RGATCY 1 cut(s) 303
PvuII CAGCTG 1 cut(s) 1151
RsaI GTAC 3 cut(s) 106, 978, 1416
RsaNI GTAC 3 cut(s) 105, 977, 1415
RseI CAYNNNNRTG 3 cut(s) 507, 1311, 1643
SaqAI TTAA 4 cut(s) 59, 1022, 1467, 1476
Sau3AI GATC 5 cut(s) 303, 387, 430, 1114, 1654
Sau96I GGNCC 1 cut(s) 752
SchI GAGTC 2 cut(s) 176, 284
ScrFI CCNGG 4 cut(s) 109, 343, 664, 738
SduI GDGCHC 1 cut(s) 257
SfaNI GCATC 4 cut(s) 124, 259, 712, 997
SfcI CTRYAG 5 cut(s) 94, 1062, 1152, 1460, 1528
SmiMI CAYNNNNRTG 3 cut(s) 507, 1311, 1643
SmlI CTYRAG 2 cut(s) 566, 621
SmoI CTYRAG 2 cut(s) 566, 621
SpeI ACTAGT 1 cut(s) 979
Sse9I AATT 8 cut(s) 402, 559, 828, 1187, 1346, 1495, 1614, 1621
SsiI CCGC 5 cut(s) 15, 37, 113, 1102, 1286
SspI AATATT 1 cut(s) 817
SspMI CTAG 5 cut(s) 446, 572, 902, 980, 1251
StyD4I CCNGG 4 cut(s) 107, 341, 662, 736
StyI CCWWGG 1 cut(s) 369
TaaI ACNGT 4 cut(s) 313, 1081, 1218, 1529
TaiI ACGT 4 cut(s) 14, 74, 223, 1450
TaqI TCGA 2 cut(s) 1239, 1521
TaqII GACCGA 1 cut(s) 166
TasI AATT 8 cut(s) 402, 559, 828, 1187, 1346, 1495, 1614, 1621
TauI GCSGC 2 cut(s) 40, 116
TfiI GAWTC 1 cut(s) 891
Tru1I TTAA 4 cut(s) 59, 1022, 1467, 1476
Tru9I TTAA 4 cut(s) 59, 1022, 1467, 1476
TscAI CASTG 2 cut(s) 300, 1645
TseI GCWGC 8 cut(s) 116, 137, 140, 143, 146, 321, 725, 1459
TspDTI ATGAA 8 cut(s) 519, 572, 677, 1145, 1158, 1200, 1295, 1458
TspGWI ACGGA 1 cut(s) 281
TspRI CASTG 2 cut(s) 300, 1645
Van91I CCANNNNNTGG 1 cut(s) 706
XagI CCTNNNNNAGG 2 cut(s) 193, 1409
XapI RAATTY 5 cut(s) 402, 559, 1187, 1495, 1614
XcmI CCANNNNNNNNNTGG 1 cut(s) 800
XmnI GAANNNNTTC 1 cut(s) 930
XspI CTAG 5 cut(s) 446, 572, 902, 980, 1251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.